LOCUS       DAZKOM010000001       118657 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000001 DAZKOM010000000
VERSION     DAZKOM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 118657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.001"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..2318)
                     /locus_tag="ACG8NF_000001"
     CDS             complement(132..2318)
                     /locus_tag="ACG8NF_000001"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0470523.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2536..3219
                     /locus_tag="ACG8NF_000002"
     CDS             2536..3219
                     /locus_tag="ACG8NF_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0470524.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3300..4019)
                     /locus_tag="ACG8NF_000003"
     CDS             complement(3300..4019)
                     /locus_tag="ACG8NF_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0470525.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4331..4537)
                     /locus_tag="ACG8NF_000004"
     CDS             complement(4331..4537)
                     /locus_tag="ACG8NF_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470526.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4782..5384
                     /locus_tag="ACG8NF_000005"
     CDS             4782..5384
                     /locus_tag="ACG8NF_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0470527.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5525..6805
                     /locus_tag="ACG8NF_000006"
     CDS             5525..6805
                     /locus_tag="ACG8NF_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0470528.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6818..7546
                     /locus_tag="ACG8NF_000007"
     CDS             6818..7546
                     /locus_tag="ACG8NF_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0470529.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7615..9138)
                     /locus_tag="ACG8NF_000008"
     CDS             complement(7615..9138)
                     /locus_tag="ACG8NF_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0470530.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9390..10571
                     /locus_tag="ACG8NF_000009"
     CDS             9390..10571
                     /locus_tag="ACG8NF_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0470531.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10631..12259)
                     /gene="treC"
                     /locus_tag="ACG8NF_000010"
     CDS             complement(10631..12259)
                     /gene="treC"
                     /locus_tag="ACG8NF_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0470532.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12710..15421)
                     /locus_tag="ACG8NF_000011"
     CDS             complement(12710..15421)
                     /locus_tag="ACG8NF_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0470533.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15431..16132)
                     /locus_tag="ACG8NF_000012"
     CDS             complement(15431..16132)
                     /locus_tag="ACG8NF_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470534.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16318..16917
                     /locus_tag="ACG8NF_000013"
     CDS             16318..16917
                     /locus_tag="ACG8NF_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0470535.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16998..18011
                     /locus_tag="ACG8NF_000014"
     CDS             16998..18011
                     /locus_tag="ACG8NF_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0470536.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18119..19036
                     /gene="rbsK"
                     /locus_tag="ACG8NF_000015"
     CDS             18119..19036
                     /gene="rbsK"
                     /locus_tag="ACG8NF_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0470537.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19064..19459
                     /gene="rbsD"
                     /locus_tag="ACG8NF_000016"
     CDS             19064..19459
                     /gene="rbsD"
                     /locus_tag="ACG8NF_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0470538.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19459..20832
                     /locus_tag="ACG8NF_000017"
     CDS             19459..20832
                     /locus_tag="ACG8NF_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0470539.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21189..23333
                     /locus_tag="ACG8NF_000018"
     CDS             21189..23333
                     /locus_tag="ACG8NF_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0470540.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23385..24425)
                     /locus_tag="ACG8NF_000019"
     CDS             complement(23385..24425)
                     /locus_tag="ACG8NF_000019"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0470541.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24702..26504)
                     /locus_tag="ACG8NF_000020"
     CDS             complement(24702..26504)
                     /locus_tag="ACG8NF_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470542.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26518..27270)
                     /locus_tag="ACG8NF_000021"
     CDS             complement(26518..27270)
                     /locus_tag="ACG8NF_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470543.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27279..27641)
                     /locus_tag="ACG8NF_000022"
     CDS             complement(27279..27641)
                     /locus_tag="ACG8NF_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0470544.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27817..28689)
                     /locus_tag="ACG8NF_000023"
     CDS             complement(27817..28689)
                     /locus_tag="ACG8NF_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0470545.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28740..29228)
                     /locus_tag="ACG8NF_000024"
     CDS             complement(28740..29228)
                     /locus_tag="ACG8NF_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0470546.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29366..30157)
                     /locus_tag="ACG8NF_000025"
     CDS             complement(29366..30157)
                     /locus_tag="ACG8NF_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0470547.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30212..30820)
                     /locus_tag="ACG8NF_000026"
     CDS             complement(30212..30820)
                     /locus_tag="ACG8NF_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0470548.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30813..31130)
                     /locus_tag="ACG8NF_000027"
     CDS             complement(30813..31130)
                     /locus_tag="ACG8NF_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0470549.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31286..32014)
                     /locus_tag="ACG8NF_000028"
     CDS             complement(31286..32014)
                     /locus_tag="ACG8NF_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0470550.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32173..33054
                     /locus_tag="ACG8NF_000029"
     CDS             32173..33054
                     /locus_tag="ACG8NF_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0470551.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33291..34334)
                     /locus_tag="ACG8NF_000030"
     CDS             complement(33291..34334)
                     /locus_tag="ACG8NF_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0470552.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34337..35005)
                     /locus_tag="ACG8NF_000031"
     CDS             complement(34337..35005)
                     /locus_tag="ACG8NF_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0470553.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35147..37483
                     /locus_tag="ACG8NF_000032"
     CDS             35147..37483
                     /locus_tag="ACG8NF_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0470554.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37598..37909)
                     /locus_tag="ACG8NF_000033"
     CDS             complement(37598..37909)
                     /locus_tag="ACG8NF_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470555.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38090..39565
                     /locus_tag="ACG8NF_000034"
     CDS             38090..39565
                     /locus_tag="ACG8NF_000034"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0470556.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39612..40196
                     /locus_tag="ACG8NF_000035"
     CDS             39612..40196
                     /locus_tag="ACG8NF_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0470557.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40198..40617
                     /locus_tag="ACG8NF_000036"
     CDS             40198..40617
                     /locus_tag="ACG8NF_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0470558.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40898..41182)
                     /locus_tag="ACG8NF_000037"
     CDS             complement(40898..41182)
                     /locus_tag="ACG8NF_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0470559.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41534..42244)
                     /locus_tag="ACG8NF_000038"
     CDS             complement(41534..42244)
                     /locus_tag="ACG8NF_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0470560.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42241..43713)
                     /locus_tag="ACG8NF_000039"
     CDS             complement(42241..43713)
                     /locus_tag="ACG8NF_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0470561.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43851..44324
                     /locus_tag="ACG8NF_000040"
     CDS             43851..44324
                     /locus_tag="ACG8NF_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0470562.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44477..45028)
                     /locus_tag="ACG8NF_000041"
     CDS             complement(44477..45028)
                     /locus_tag="ACG8NF_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0470563.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45133..45663
                     /locus_tag="ACG8NF_000042"
     CDS             45133..45663
                     /locus_tag="ACG8NF_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0470564.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46146..46589)
                     /locus_tag="ACG8NF_000043"
     CDS             complement(46146..46589)
                     /locus_tag="ACG8NF_000043"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0470565.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46605..47765)
                     /locus_tag="ACG8NF_000044"
     CDS             complement(46605..47765)
                     /locus_tag="ACG8NF_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0470566.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48272..49972
                     /locus_tag="ACG8NF_000045"
     CDS             48272..49972
                     /locus_tag="ACG8NF_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470567.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50074..50907)
                     /locus_tag="ACG8NF_000046"
     CDS             complement(50074..50907)
                     /locus_tag="ACG8NF_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0470568.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51211..53376)
                     /locus_tag="ACG8NF_000047"
     CDS             complement(51211..53376)
                     /locus_tag="ACG8NF_000047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0470569.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53636..55930
                     /locus_tag="ACG8NF_000048"
     CDS             53636..55930
                     /locus_tag="ACG8NF_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0470570.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55923..56588
                     /gene="pgmB"
                     /locus_tag="ACG8NF_000049"
     CDS             55923..56588
                     /gene="pgmB"
                     /locus_tag="ACG8NF_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0470571.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56581..57588
                     /locus_tag="ACG8NF_000050"
     CDS             56581..57588
                     /locus_tag="ACG8NF_000050"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0470572.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57610..58629
                     /locus_tag="ACG8NF_000051"
     CDS             57610..58629
                     /locus_tag="ACG8NF_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0470573.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58910..60277)
                     /gene="nhaC"
                     /locus_tag="ACG8NF_000052"
     CDS             complement(58910..60277)
                     /gene="nhaC"
                     /locus_tag="ACG8NF_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0470574.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60386..61807)
                     /gene="tyrP"
                     /locus_tag="ACG8NF_000053"
     CDS             complement(60386..61807)
                     /gene="tyrP"
                     /locus_tag="ACG8NF_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0470575.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62050..63927)
                     /gene="tdc"
                     /locus_tag="ACG8NF_000054"
     CDS             complement(62050..63927)
                     /gene="tdc"
                     /locus_tag="ACG8NF_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0470576.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64218..65474)
                     /gene="tyrS"
                     /locus_tag="ACG8NF_000055"
     CDS             complement(64218..65474)
                     /gene="tyrS"
                     /locus_tag="ACG8NF_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0470577.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65586..65791)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66054..67355
                     /locus_tag="ACG8NF_000056"
     CDS             66054..67355
                     /locus_tag="ACG8NF_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0470578.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67424..68023)
                     /locus_tag="ACG8NF_000057"
     CDS             complement(67424..68023)
                     /locus_tag="ACG8NF_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0470579.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68277..69158
                     /locus_tag="ACG8NF_000058"
     CDS             68277..69158
                     /locus_tag="ACG8NF_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0470580.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69171..70781
                     /locus_tag="ACG8NF_000059"
     CDS             69171..70781
                     /locus_tag="ACG8NF_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470581.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70858..71517)
                     /locus_tag="ACG8NF_000060"
     CDS             complement(70858..71517)
                     /locus_tag="ACG8NF_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0470582.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71800..72465)
                     /locus_tag="ACG8NF_000061"
     CDS             complement(71800..72465)
                     /locus_tag="ACG8NF_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0470583.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72612..73217)
                     /gene="dhaL"
                     /locus_tag="ACG8NF_000062"
     CDS             complement(72612..73217)
                     /gene="dhaL"
                     /locus_tag="ACG8NF_000062"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0470584.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73245..74255)
                     /gene="dhaK"
                     /locus_tag="ACG8NF_000063"
     CDS             complement(73245..74255)
                     /gene="dhaK"
                     /locus_tag="ACG8NF_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0470585.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74230..74625)
                     /gene="dhaM"
                     /locus_tag="ACG8NF_000064"
     CDS             complement(74230..74625)
                     /gene="dhaM"
                     /locus_tag="ACG8NF_000064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0470586.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74779..75099
                     /locus_tag="ACG8NF_000065"
     CDS             74779..75099
                     /locus_tag="ACG8NF_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0470587.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75211..76068)
                     /locus_tag="ACG8NF_000066"
     CDS             complement(75211..76068)
                     /locus_tag="ACG8NF_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0470588.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(76176..77480)
                     /locus_tag="ACG8NF_000067"
     CDS             complement(76176..77480)
                     /locus_tag="ACG8NF_000067"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0470589.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77477..77986)
                     /locus_tag="ACG8NF_000068"
     CDS             complement(77477..77986)
                     /locus_tag="ACG8NF_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470590.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78161..80296)
                     /locus_tag="ACG8NF_000069"
     CDS             complement(78161..80296)
                     /locus_tag="ACG8NF_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0470591.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80379..82034)
                     /locus_tag="ACG8NF_000070"
     CDS             complement(80379..82034)
                     /locus_tag="ACG8NF_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0470592.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(82036..82350)
                     /locus_tag="ACG8NF_000071"
     CDS             complement(82036..82350)
                     /locus_tag="ACG8NF_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0470593.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82520..82780
                     /locus_tag="ACG8NF_000072"
     CDS             82520..82780
                     /locus_tag="ACG8NF_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0470594.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82794..83108
                     /locus_tag="ACG8NF_000073"
     CDS             82794..83108
                     /locus_tag="ACG8NF_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0470595.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83838..84392)
                     /locus_tag="ACG8NF_000074"
     CDS             complement(83838..84392)
                     /locus_tag="ACG8NF_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0470596.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84518..86447)
                     /locus_tag="ACG8NF_000075"
                     /pseudo
     CDS             complement(84518..86447)
                     /locus_tag="ACG8NF_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(86753..87469)
                     /locus_tag="ACG8NF_000076"
     CDS             complement(86753..87469)
                     /locus_tag="ACG8NF_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0470597.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87470..89293)
                     /gene="glpO"
                     /locus_tag="ACG8NF_000077"
     CDS             complement(87470..89293)
                     /gene="glpO"
                     /locus_tag="ACG8NF_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0470598.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89307..90803)
                     /gene="glpK"
                     /locus_tag="ACG8NF_000078"
     CDS             complement(89307..90803)
                     /gene="glpK"
                     /locus_tag="ACG8NF_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0470599.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(90939..92399)
                     /locus_tag="ACG8NF_000079"
     CDS             complement(90939..92399)
                     /locus_tag="ACG8NF_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0470600.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92389..93684)
                     /locus_tag="ACG8NF_000080"
     CDS             complement(92389..93684)
                     /locus_tag="ACG8NF_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0470601.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(94016..94693)
                     /locus_tag="ACG8NF_000081"
     CDS             complement(94016..94693)
                     /locus_tag="ACG8NF_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0470602.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94723..95814)
                     /locus_tag="ACG8NF_000082"
     CDS             complement(94723..95814)
                     /locus_tag="ACG8NF_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0470603.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(95915..96349)
                     /locus_tag="ACG8NF_000083"
     CDS             complement(95915..96349)
                     /locus_tag="ACG8NF_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0470604.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(96933..98390)
                     /locus_tag="ACG8NF_000084"
     CDS             complement(96933..98390)
                     /locus_tag="ACG8NF_000084"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0470605.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98387..100282)
                     /locus_tag="ACG8NF_000085"
     CDS             complement(98387..100282)
                     /locus_tag="ACG8NF_000085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0470606.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100340..102115)
                     /locus_tag="ACG8NF_000086"
     CDS             complement(100340..102115)
                     /locus_tag="ACG8NF_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0470607.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102165..103661)
                     /locus_tag="ACG8NF_000087"
     CDS             complement(102165..103661)
                     /locus_tag="ACG8NF_000087"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0470608.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103855..105477)
                     /locus_tag="ACG8NF_000088"
     CDS             complement(103855..105477)
                     /locus_tag="ACG8NF_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0470609.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105805..107118)
                     /locus_tag="ACG8NF_000089"
     CDS             complement(105805..107118)
                     /locus_tag="ACG8NF_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0470610.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107363..107587)
                     /locus_tag="ACG8NF_000090"
     CDS             complement(107363..107587)
                     /locus_tag="ACG8NF_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470611.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107607..108029)
                     /locus_tag="ACG8NF_000091"
     CDS             complement(107607..108029)
                     /locus_tag="ACG8NF_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0470612.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(108171..108881)
                     /locus_tag="ACG8NF_000092"
     CDS             complement(108171..108881)
                     /locus_tag="ACG8NF_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0470613.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(108899..109396)
                     /locus_tag="ACG8NF_000093"
     CDS             complement(108899..109396)
                     /locus_tag="ACG8NF_000093"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0470614.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109433..109885)
                     /locus_tag="ACG8NF_000094"
     CDS             complement(109433..109885)
                     /locus_tag="ACG8NF_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0470615.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(110020..110625)
                     /locus_tag="ACG8NF_000095"
     CDS             complement(110020..110625)
                     /locus_tag="ACG8NF_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0470616.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(111075..111695)
                     /locus_tag="ACG8NF_000096"
     CDS             complement(111075..111695)
                     /locus_tag="ACG8NF_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0470617.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111697..112566)
                     /locus_tag="ACG8NF_000097"
     CDS             complement(111697..112566)
                     /locus_tag="ACG8NF_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0470618.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112589..112996)
                     /locus_tag="ACG8NF_000098"
     CDS             complement(112589..112996)
                     /locus_tag="ACG8NF_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0470619.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(113015..114304)
                     /locus_tag="ACG8NF_000099"
     CDS             complement(113015..114304)
                     /locus_tag="ACG8NF_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0470620.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            114362..115701
                     /locus_tag="ACG8NF_000100"
     CDS             join(114362..114803,114803..115701)
                     /locus_tag="ACG8NF_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0470621.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            115813..116709
                     /locus_tag="ACG8NF_000101"
     CDS             115813..116709
                     /locus_tag="ACG8NF_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0470622.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            116801..117016
                     /locus_tag="ACG8NF_000102"
     CDS             116801..117016
                     /locus_tag="ACG8NF_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0470623.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            117034..117306
                     /locus_tag="ACG8NF_000103"
     CDS             117034..117306
                     /locus_tag="ACG8NF_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0470624.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            117308..118171
                     /locus_tag="ACG8NF_000104"
     CDS             117308..118171
                     /locus_tag="ACG8NF_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0470625.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT        35162 a        24307 c        20782 g        38406 t
ORIGIN      
        1 ccgaataaac tgtgttccag aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat
       61 ttgagaagca attttcgtac attccttctt ctttcttgaa actgaccact tctgtcacac
      121 cctctcttct atcactttcc ttttttcaca cgactcccgg tttctttgat aaatctcgat
      181 actttcaatt ttcttgaatt tttcatttga ggtacacata gatacagacg atttcttgct
      241 ctagtcatcg caacataaaa tagtcttcgt tcttcttcta tcgtcgttac tggttctgct
      301 tttatttcat taaagtcgat tactttcttt tctgtcgctc cgttataagg caaacttcct
      361 tcgttacaac caatcaaaaa gacatttcgg taactaagac ctttactttt gtgaatgctc
      421 tggatacata gtgcatcaag ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct
      481 ttagctgtcg cttgggtttc ttgcgataag gcttggagtc ttctgaaagt agcatgctgc
      541 tttacttctt ctcgtatcca ttcttgatca ctctggtcga tttccttagc tatacgtggc
      601 agatgaagat agctttgaac aagcgaatag atttcttgat gctggtctgt tttacgcaac
      661 gcttcctcaa actctcggat ttctgcagat ttcgatttta aattggggct tatcgacaaa
      721 atataatctg caacggctat attttgatat ttaggagaaa agtattgatc gcattcactt
      781 tttttcaggt agcagtctgc catcagctgc cggagcaatt gctggtaggc ctgatttttc
      841 ttttgcagat tcagtgctgt ttgccaatga acaatttttt gatagaaatg ttggaaagac
      901 ttgataccaa agataccttc atatggacta tctgcttcca gtacaaaagg aatattcgtt
      961 ttcagcagag attggacaag ctgctgcgtt tgtgaagctg atcggtataa tatgattgat
     1021 tccttatatt tcccattttt ttgaataaat gtctgaattt gtttgattag ccactgactt
     1081 tctgctttag tatcagaaaa caaataggct tccataacac ctgcttcttt ttgtgctgga
     1141 atcatcgcct ttggtatccg atcattttta ttatagctga tcaatctatt tgctccttgg
     1201 acaatagctg cccgagaacg ataattagtt aataagaaaa gagttttggt aggatattct
     1261 ttttcaaagt tcttgatgta tgctggctct gatcctctga atccatagat actctgatca
     1321 tcatctccaa caacaatcaa cttgatcctg ttttcctttg tcatccgttt cacgatttcc
     1381 cattgaagtg gattagtatc ttgaaattca tcaatgaata gataatcata tttttggccg
     1441 atataatgct ggatctcctc attctccaaa gcgcttttca gattttggag tacatcattg
     1501 aactctagca attgctggct tcttttttgt tcttggagtt ccgtgagcaa tccatacagt
     1561 cgttctgata tcggctgtcg tgcattctta tcaatggaat catatttttc tttaactaac
     1621 tgaaccattt cttctactga aaggttcata ttctgccaat agtctaaccg agaagcaatc
     1681 gttttttctc catattgatc aaatatataa agattatatt tacgtatcag ctgttgcaaa
     1741 acagcattca ctttttgtgc agtatccttt gaaaagtctc cttgtattcc ttgcattgct
     1801 acttcaggaa attttcgaca aatttcattg tatatcctaa aaaagatact atggaatgtt
     1861 ccgatatgga cagcggtttt tctctggctg aaattatcta ctcgtttttt tagttcattt
     1921 gcagcgttcc ttgtaaaagt gatcattagt atgcgttctt cgtctacttg tttttgcgtc
     1981 atcagataaa cagcttttgc acagattgtt tgcgttttgc cgctcccagc tcctgctatg
     2041 attcgaagat gtttcttcat atcatatgtg atagctgctt tttgttcttg attgaagcga
     2101 ttataggaac gtttctccat cattttaaag aaagagctgt ttccttcact ttcttttact
     2161 tgtacttctt cctcaataag tccgggtgct tcgccataag ccgcttcata ggtagcaatg
     2221 atttcttctt gtagccgaaa gacttgttga agctttttat acttgttctc ccagcgtttc
     2281 aattcttttt gtaatcggag aatttctttt tcattcaact gattgatttc cttcccatct
     2341 ttcttctttg aatttgttta tttcaagttg gttatttaaa gtttcgttgt ctattttttc
     2401 tttatactaa cagacctttc taaaaatgaa aatgggaagc gatatgattt tagttttctg
     2461 aaaatttttc ctttttttca atcgtttatg ctaagataag gaagacaaaa atattcataa
     2521 aaggtgatga aaaaaatgca gacttccttt cacgaggggc gtacaatagg acttttgctg
     2581 acatttatcg gaggagccat ggacagttat acttatatcc aatatggtgc ttttgcctct
     2641 gctcaaactg gcaatatcat tctatcgatc atccaagcat ttgacggaga atggctaagt
     2701 gttggtaaaa aagtattgtc tacccttttc tttttcttag ggattcttct aacaaagtat
     2761 ctaattgatt attttagaaa aaaagaaaaa catttctggc gcttgtttgt tctatattat
     2821 gaagcagtga tttttttctt tgttagtttg acacccgtcc atgagcatcc cgcattagta
     2881 acgattttga ttgcttttac tgcatcgatc cagtgggtag cttttgataa aatcaacggg
     2941 cgtgcctaca ctaatttatt tacaactgga aatttaaaag gggtagcaac gaacttatat
     3001 gattattgta tttctaaaga aaaagcagat tttgatcgat ttttccacta tctaagtgtc
     3061 gttcttgcct ttatcgcagg agctattgtc cttgttttct gtcatcatct gtttggcgcc
     3121 cattcgattt tgatcgtttc cattttattt ttgatcatgg ccctcactca gacatttcat
     3181 ttatggcaat tttatcatac gaatcggttc actatttgat ctcaatgaat caaaaaaact
     3241 aagcaagatg ctgctcacag agaaatatct gcgagtgagc aatttgctta gtttttatat
     3301 tatttagttg taaagattcc ctttgctttc gggttatttg tagtgtagcg gatcgactgg
     3361 gcatctgcct cttctgtcat tttctttgcc cgcatcttga tttcttgtac gatttgttct
     3421 ccacatgccg gatcggctac gaaatcatat ttgtccccat cgatcactat tttgggacat
     3481 atatcaaact gttcatacca ctcttcgtat cgtcgattga gttctttata gtaggaatag
     3541 agttcaggat cttgttctag ctgttcatat tcccggcctc tttttttgat tctcgctaac
     3601 atagtttcaa aggatacacg aatataaacc agtagatctg gtttcttttg tggcgaaatt
     3661 tcttctaact ccttcagcat cgtttctaaa agaccttcat actgctttac ttctacttca
     3721 gaaacacgcc ctaaatctgc attcaaatga aacagcatac tatcttcgta gatggagcgg
     3781 tctagtacgt tgtcatcatg gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa
     3841 aagaaaatct gtaataaaaa agtatacttt tctggattag aataaaatag cggcaatact
     3901 tcattatctt ccacgttctc ataaaaaggt tttgtttcta aatcttgggc aagcatttca
     3961 gttagtgtgc ttttccctgc accgatcgtt cctgcaacaa tgatcacaat tatcggcatc
     4021 cttcctgtaa taaaatctgt tcttcatttt actacttcat ttatagaaat aaaagccttg
     4081 actttatctg ctccttaaaa gctacttctc taaaagtagc caaaagtaac tcctattcat
     4141 gaaaataaag gctgtgacaa aagtcttgtc gcagcccttt tttcaaataa acagtattct
     4201 cctgccaaca cctcggtcac aaggcaatat tcataaaaca tgaaaagctg ttttttaaca
     4261 ttattccttg taactcagag ctaaacggga gactcacaac ctctttatgc tctcaacatt
     4321 ttatctgttt ttattcttct attgtacttg atttgcgtac aactaactgc cagtgcagat
     4381 tatgagcggt atcattgtct tctactgcgc atttcatgac acgtatcatc acactatttt
     4441 catagatttt ttctattttt ccttttattg gtgattcaaa taaatcagat ttaaatgcat
     4501 atgtttcgcc gacttcaaac aattgttcat tttccataag aatccctcca tcacctttta
     4561 taaggtcagt aattttttaa cataaatcac tcgaaaaagc acaaacttgt cataaaaagt
     4621 acttttcgta tcaaaactgt cacttttttt ggtaaggttc ttggaaaacg aagatttttt
     4681 taggagatca tgacggtttt gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt
     4741 atttttttta gcataaaatt tgtggtggag gtacgtatga gatgaaagat aaagttgtga
     4801 acaaattatt agaagcctta ttcagcggag acaaatcgga agaagatatg gtgtatgtcc
     4861 aagtaaaatt tgtcttagaa gttttattga ataatcttgg aaaactagcg gtcgttctag
     4921 tcttttctct ggtgacaggc tcatgggcgg aaacaggaat cacctttctt tcttatatct
     4981 gcatccgcag atatgcttat ggattgcatt ccgactctga attcgtttgt ctgctgtgga
     5041 cactacttta tttatggggc gttcccttgg tgatgaaaca tctgcaattg actatctcct
     5101 tcccaatgat ggtctttttg ttaatcagct gctttttgtt actgcttcgc tatggatcaa
     5161 gaggtacagc aatcaatccc attgaacccg agaaacgtcc cccattatta aagaaggcga
     5221 tcagcatgtt tctaattttt tctctcatta cgttattttt ctcagcttct tacttttcta
     5281 cctatttgct acttgggatt gttttagaaa tagctacgtt gcttcctatt acaaactact
     5341 tgatgaattt tggaggaatc tttcatgaaa aaaacaatca ttaaacaatt aactaactta
     5401 tctgactttt ttgtatctgg ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa
     5461 aaattaaaaa aataatacgc caattgtgct ataatttggt aaaaatcaat gggagctgta
     5521 ctttatgttt ttatggtcca tagcgttgca atatttgatt tacacaattt gtatcttatt
     5581 gattgaccat cacatccgat acaaatatct gtttgcggga gtcattctga tttcttgtat
     5641 tttgattgga tgggtctatt cgattttagg gatttttact gctctaattg ctataggaat
     5701 gctgatgctt acttttttct tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc
     5761 ttttgggtta ttgacgtcgc ttttaggaga tcacttaaca agcatcggcg atttttttgt
     5821 tttgaaatct tcggttattg aaccaggaga ttcattgcag tattttcaca ttgtcttatc
     5881 cgctttttta tctgctttat tagctttttg tttaaaaaaa tcgttagaga cttggtttca
     5941 cactgtggac cgccgtatga tcggtacgat cgggacattg atattattta catactatat
     6001 cattattttt tatacccgtt tttctggtga aacacctgat gtcttagctt tgaatacgag
     6061 ttttttcatt ctatatctgt gtgttggcct gatcatcttg gtttattatt ggaaaatctc
     6121 taataagaaa ttagctgatc agttattaga gcaacaagtc aggatgcaac aggattatat
     6181 caaagatcta gaaaaaaact atcaagactt gagagaattc aaacatgatt accaaaatct
     6241 gcttttttct cttcacagct atatttcaga aggcgactta gaaggattac ggtcttatta
     6301 tgatcaaacg atattaccta cacgccagat gatgaacctt tttccggcca atctaagtat
     6361 gttggataat atgaaagttc ctgagatccg tagtttactg tcattaaaac tcatgatggc
     6421 acaggaaaaa gggttgatcg tccagcttca ttttcctgag ccaatcgaac tggatacgaa
     6481 acatacggta aaccttgtac gtatgcttgg tattatcttg gacaatgcga ttgaaggagc
     6541 tagtgctgca aagaagaaaa aaatcgaatt gaccatactc aagaagaatc attccgtgat
     6601 gattcgtgtg atcaatacaa caacaaatac attaccgtta aaccagttga aacaaaaagg
     6661 gttctcaaca aaaacaaatc caaaaaacga aggccttggc ttgtttatct tggatgaatt
     6721 gacaaagacc aatccttatc ttttccttga aacatcaatt gaaaaccagc gtttttctca
     6781 aacgatttat atacaaataa aataaaggag taatgatatg attcccattt atatttgtga
     6841 agacgatcca aaacaattag aagttttatc aacggtgatc aaaaatagaa tcatgattga
     6901 aaacttagac agttatgtcc atatttctac ctctgatccg gaagaacttc tcaatgcagc
     6961 caagatcaga acttcttctt ttggtattta ctttttagat atcgaactag aagacagtga
     7021 aatggatggt gtagcgatcg gtaaattgat ccgtgaatta gatcctttag gaaaaataat
     7081 ctatgtgact tcccatacgg aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac
     7141 tgattatatc gtcaaagaag atctttatga tttaaaagaa aatgtcgagc atatcctgag
     7201 caaaattttc gaagattttc aaatgtccca aatccaaaaa gatatcttca aaatagagtt
     7261 taatgatgag atcaaatttt taccgatcaa agacatccaa tatttttcta cttcgctagg
     7321 agcgccacat aaactggaag tccatttgac tcatgcccaa ttacaattct atgagaagat
     7381 caaagaaatc gagaaaatgc atcggaattt tatccgttgt cataaatcct atgttatcaa
     7441 tacacaaaat gtgcggtcga ttaataaaaa aaccagagaa gtcacgttgg caaacggcga
     7501 gaccatccca gcctctatcc gtggattaaa gaaattgatt tcataaaaag aaccgaatag
     7561 tttggaccga ctaaatgacc agccgatcca aacccttcgg ttcttttttg cctatcagtg
     7621 caaaagcatt tcctgtgcaa tttcacgtcc atccattgta tctggataaa atgttggcca
     7681 attctccatt tctttcagca atacttcttg actttcattt cctgcatata aatgaaaatg
     7741 ttcggagtca gccggtgcaa tcgtgtgatc actgaattga atatatctaa aagcgccact
     7801 ttccttatct tctgcttcga atagatacct tacgccacgg tttcctgcct cgtaggtcaa
     7861 gatttctttc cctacataac gataatttga acgtttcgtt gttccattct taaacgtaaa
     7921 tgccatctct ttctcactga tcttgattga agcaatatct gtttgatagc ccttcgtata
     7981 atattcttta tattcttgtg ctgtcttatc ctgattcaat ttttctttat attcaaaaac
     8041 aggatcaaaa gtcccatctt ctacatacgt atctactgtt tgccaagttc ctgcccaatc
     8101 tgaaagagag cgatcttgta catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc
     8161 ttttttgggg gtcaaagtat cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc
     8221 ttcgaggttt gcttccatga tagaaagata gttttctcca ttttctattt gttcattcgt
     8281 caatccttcc aaaggattca atacttctaa agatacacct gcttcgtttg ctaacgttct
     8341 ggcaatacta tctttggcgt tttcttcaaa ataaatatag ttgattccat gttctttaac
     8401 aaagtgtttc agttcagcaa ttctcgccgc agacggttct tcactagaag aaagaccagc
     8461 aatcgatact tgattcaatc catattctaa cgctagatag ttgaatgcag catgttgcgt
     8521 gacaaaagat ctttgttttg cttgcttaaa ggtttcttga taaagctgat ggagtgcttg
     8581 aagtttctcg gtataggctg ctgcattttt tgtccatact tcctgttttt ctgggtaagc
     8641 ttcaatcagc tgatcacgta tgtttgctac ctgcttgatt gctaaacttg gagctagcca
     8701 tacatgtggg tctagctcat gtgtatggcc ttcttccccg tgttcatggt catgatcatg
     8761 tccttcttca cttcccggaa gtaaaagcat accttctgtc gcttttatcg tatggacatt
     8821 gccttcttgt aatgtttttt ggattgcggg gacccatgtt tccatgtttt catcattata
     8881 aacaaagata tctgtatctt ggatctttgc catgtcttta gcagaaggtt cataatcgtg
     8941 ggaatcagtc cctgctggaa tcaataactc tacttctcct tcatccccga cgattgcttt
     9001 tgtaaaatca tacatgggat aaaaggtcgt catcacgctc aatttttctt tactgtcctc
     9061 ggctttccca cttgccccgc aagcagttaa catacctgct aaaaccagca tggccaatat
     9121 acctatcaat cgtttcattt atgacactcc tcttctcatc ttctttcatt tgaatgttcc
     9181 gattcgcttt tcgcaaaccg taatagttac gttttagcaa caggatttaa tttatcatat
     9241 ggttaaaaga actgtcaagc gttttcttat tttaattgat gagaaagttt atatgaatga
     9301 ttgacgatat ttttgcaatc aacctctatc attttccttt tatacccgct tttgtctata
     9361 atgaatttat agataaagga ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt
     9421 tgatccgctt tttaggagga aaaacctctt actatgtttt aggaatagtt atcttacttg
     9481 cgctgatcat ttattttttc catcagatct cgtttatatt caagcctgtc ggaatcatta
     9541 tcgctactat cttgccccca ttattgtttg cactgatctt gtattatctt ttggaaccag
     9601 tcatcaaacg tttgagcaag cacctttcga ggaacttgtc agtcattatc gtttatgtga
     9661 ttattttatt tttattgggg cttggaagca tatggcttat tccttttctt gaagaacagg
     9721 caaagacatt gatcagtagt ttgccagatt tgtttcaaga ttttcaagag agtctccgac
     9781 aatttttgga gaaaacacct tttgccgatt catttaatca atttttcgct tctattgacg
     9841 acgtgacgtc tgatatagct ggatttattg gaaattattg ggaaagtggc gcacaacgga
     9901 tcagcaacat tttttcaact gttacagcta tttttattac tttatttact ggtccaattg
     9961 tcgctttttt ccttttgaga gatcctcaaa aattttatca gtcagtttta gcgattgtcc
    10021 caccagcatt tcgtacagat tttaaaaatt tgacaaagat tgctaatcaa caattgggtt
    10081 catttctaaa ggggcaaatc attgcttcat ttatccttgg agcagtctac tgggtttgtt
    10141 tcttactgat cggattagaa tttgctagtg ttttagctat ttctgctggg ttgttatgta
    10201 tcattccata tattgggccg tttatcgcct tctttccagg tttgatcatt gcattccaag
    10261 actctacttt tatggctgtc aaatttgtca ttgtctggtt tgccgtacaa cttctccacg
    10321 gtgacttggt gattcctcga gtaatgggtg atcgtttaca gatccatccg attacgatct
    10381 taatcgtttt acttgtcatg ggtgatctaa tgggaattgt gggcgttatt tttggtattc
    10441 ctatctatac gttagtcaaa ttactcgtta cttttatttt ccgtaaattc aaacaacgct
    10501 acaataaatt ttatggcgat aaaggagagt atgaaaactc ttcattttct gaagaagatt
    10561 attttaaata aaacacaaaa cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg
    10621 tgtttttgtt ttaatttttc agcaaagcaa ttgcctggta tggttttagc tccagacttt
    10681 gatgaagaac agtatcggaa taattatcga taagaatatt ggcttctgtg aatccttctg
    10741 ggatggcgat ccttgtatct ttatcataga aatttgttaa tacaagcagc tgctgtcctt
    10801 caaattggcg aagaaagcca taaacttccg gatgatttgc ttcataagca tgataatctc
    10861 cttgtgcaac gattggatat gtttttctca gccgaatcaa tttcttgtaa taggacaaaa
    10921 tagaatcttc agaagatagt tctttttcta cattaatctc ttctagcttg cctccaactt
    10981 ttaaccaagg tttccctgta gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg
    11041 ttcgagagtt atcgcgagat tttgctttga tccgacgaaa cgcttctttt tctgtgaatc
    11101 cttgttcaag gagtaactta taagcattca aactttctac gtcaatatag tcggaaatcg
    11161 atgaatagtc tgggtcgatc atgccgattt cttctcccat ataaatatag ggcgtcccac
    11221 ggttcaaatg gatcatggtt gctagcatct ctgctccttg ttttcgatac tgatcgatag
    11281 ctacaaatcg attgagcgca cgtggctgat catgattatt aaaaaataga gcattccagc
    11341 cgttaccctc actcatcttt tctccccatg tatgaaatag tcgctttaat tctgcaaaat
    11401 caaatggagg agtagaccat ttattcccat tttcataatc gactttcaaa tgatggaaat
    11461 taaagaccat tgataattct tctctactag gttctgtata caagatacaa ttttcgatcg
    11521 tcgtagagct catttcaccg actgtgatga tagtggaatc cgtcccaaac gttgcttggt
    11581 tcaattcttt tagatacgta tgggtgattg gtttgtccgt atattcaggt ttgccatcat
    11641 tttctttatt atttttcaat tgttcgtctt taccgataac attgataaca tcgaaacgga
    11701 atccttttac ccctttttcc atccaaaaac gaacaacttt gaatagttcc tctcttactg
    11761 cttggttccg ccagttcaag tcagcttgtg tccgatcata taagtgcaaa taatatttcc
    11821 ccgtatctcc aaatggcgac caagcacttc caccaaattt tgatacccaa tccgttggct
    11881 cttctcttaa aatgaagaag tcctgataat gctggtcccc tgctagtgct tttttgaacc
    11941 attcatgttc tgtcgaaacg tgattcagca ccatatcgag catgatttct atccggtatt
    12001 tctttgcttc ggctattagc tgttcgaatt cctccatcgt accaaagata ggatcgatgg
    12061 ctgtgtaatc agagatatca taaccgttgt ctttttgagg gcttggataa atagggttta
    12121 gccaaatcat gtcgatgcct agatcattca gatacgggag tttttctttg attcctttta
    12181 aatctccaat tccatcacca ttactgtctt tgtaagattt gggatagatt tggtaaatga
    12241 ctttgtcttt aaatgtcatg gatatttacc tccattcaac tgatttatta cctaccacaa
    12301 cttatatata caagttatca tttattatta taactgaacc gaaagcgttt gtaaaattta
    12361 tctttataca ttctaagagg tctgttgccc aataaaaaac aagagatcag acatgtattt
    12421 gtctatcctc ttgtttaaaa tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa
    12481 agcttttatc tacggcaatc agtcttctca aatgtaaaac atgaggagct aattttattt
    12541 gagtattctt tttgctcgat gcaggacggc ttttttacaa cctctgattc atggtgttca
    12601 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaaaaact attttgccgt
    12661 ttgtttctct aatactcagg tcaaaccgct gatttcacaa cctctgtttt tattcattcg
    12721 atttcaacgc atcaatcata tcgatatgct ttaatttgaa atgggtaaca atcatcacaa
    12781 taaccgtaaa tacgatagtt aatcctgctg ataagacata agcccaccat gtgatcacta
    12841 atggaaagat gacattctcc atagacgctt gttgcaagat gaagtcagtc aatatatatc
    12901 ctacaccaaa tcctccgatg attcctaaaa gagtaaagat gatattttct cgaacaatgt
    12961 acatcgtgac ttctttatca aaaaatccaa ggacttttat tgtagataat tcgcgtacac
    13021 gctcggaaat attgatatta gttagattat acaagacgat aaaagccagc agtcctgata
    13081 atacgacaaa gattaagaca accgagtcta agttcgccat tgattcttct tgcgtttcga
    13141 tttgtttgga catgaaagac gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa
    13201 gcatttcttc ttcttttgtt gtcatcttat ctgtacgcag caaaaatgca ttatccatca
    13261 tcgattgatc agtaactgaa gcataatatt caggggacat ataaataaag ctacctagat
    13321 agtttttcac gatttctttt acttcgactt tccattcttt ttgatcttca tcataaagcg
    13381 aaatatgatc tcctgtagat aagtcaaata gttctgctac tttttgcgta atagcgattc
    13441 ctgttttagg caaggagcgt gggttctctt caagatcagt taaatggata aaacgatcca
    13501 aatcagctgt tttacttggt acgatcactg tcagactttg cgaagcatgt cctttttttc
    13561 tcaattctac ggtttgagta tgaatagcta ggatctcatt tactttttct tgcttctgca
    13621 gcatatccgc tgctttttga cgactatctt ccgtggatga atccaatgta acgattgctt
    13681 gatagtcaat gatcggtcca aattgtttgg ctgaaacaga acttagagaa tctttcaatc
    13741 ccacgcctgc aaccatcagc ccggcacatc cagcaattcc gataatcgcc ataagcattc
    13801 ttgacttata tcggaagaga tttcggtaac tgattttttg attaaagcta aggcgtgacc
    13861 acagcggagt gatgtattca agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa
    13921 gcaatgcagc aggccgttct cttaaatctt tgaataaaac gatcattgca gcgccaaatg
    13981 cggcaatgaa gaatgcggca gctgcttgta aaatcggcgg ccagtcataa aaaataagcg
    14041 cactcccaat atcataccgc tcgttagaca gctcaaaaat gatccttggc aaaagattcg
    14101 ttcctaatat cgttcctaaa acaataccta tccctgaggc tagacttgca tagattgcat
    14161 atttttttgc gatttcaact ttcgtataac ctaacgcctt taatgtcccg atttccctgc
    14221 ggttttcttc taccatacgt gtcattgtcg taaaggtgat caatgcggca ataaagaaaa
    14281 agaaaacagg aaaaacattg gcaatcgcag cgatacgatc agacagatca ccaaattctt
    14341 gaaaaccgac attttctgaa cgttcattgt ataggtatga tggtttagtt aaattttcaa
    14401 ttttttctgc atttcgattc aattcttcct ctgcagaggc cagttcacgt tctttctcaa
    14461 ctaattgcct ttcttgttca gcaagttgag cttcttgttg agataatgtt tgaacagcga
    14521 ttgttcgttg cgcttcagga agctgctgga tagttgctga ctgttgcgct aactgttttt
    14581 tagcatcttc taaccgtgtt cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt
    14641 gctgtgccgg ttctaattct tcgttggccg attttttcag ctcttctaag cgttcttcgg
    14701 gtcgatcttg taaatacttt tctgcctttt cttgattttg ctccatttta tctttgtaag
    14761 ctttgctgta cgtattcaaa ttctttacgt ttgtaaaatc aagatataag acagaataaa
    14821 tatctgtttt aaaaacggat tcaggtaaat agacaaaata atcgacgcta ccatttccga
    14881 cgttcgcgta tcctctctcc aaattgttga tgaacaatgg agaacggaca aagcccgtga
    14941 tggtatactc tttttctttc aattcatctg aatcaatctg ataaatatca ccgattttat
    15001 acccttcatc ttccgctttt tcatccaata caagctggtt tggtttttcc gggagttttc
    15061 catcagttaa ttgtagcttg ttctgcttgt tatttttgtt ataagaaaat atttgaatca
    15121 cttcgttttt gccagtattg gaataataga tctgttttcc tgtttctact tttgcatctg
    15181 ggatcttttc tgcttgagca atatcttttt gtgtcaagcc accagtagaa acgatttgca
    15241 aatcagaaag tcctgctgta ccaacatagt gatccatcgt tttttgcatg acaggtccag
    15301 cagttttgac acctacaaat aaaagtgttc ctagcatgac aataaggatg attgcaatga
    15361 atcgtccttt agattgccga atttcgcgaa aacctgcttt aagatatgtt cttgttttca
    15421 taaaatcccc ctaccataca atctcatcaa tggatgaagg atgatcattt aattcaacag
    15481 accttacttt agcatcatta atgtgaatga ctctatcagc gatgggcgct aaagcagaat
    15541 tatgtgtaat gatcaaaacg gtattgccgt gttgtctgct agcgttttgc aataatttca
    15601 atacttgctt gcccgtttca aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt
    15661 tgggattttt agctagtgca cgagcaattg aaaccctctg ctgttcgcct cctgataatt
    15721 gggacggaaa gttattcaat cgatgagcta ggcccacttg cctcaatacc tctacaggat
    15781 caagcgcatc aggagaaact tccgttgcca attccacatt ttcttttgct gttagattag
    15841 gaacgagatt gtagaattgg aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct
    15901 gtttatctga aaattgcgca atatctgtgt catcaataat gatctgacct tcatcaggtg
    15961 tatccatccc acctaaaata ttaagaatcg ttgactttcc tgctccactt ggaccaagta
    16021 taatcactaa ttcgcctttt tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg
    16081 tttctcccat tttgtatcgt ttgtattcat tttttacttc gacatacttc actctgattc
    16141 ctccattttc acgatatttc tcgctatatc tatgcatcga atgcgttttc tcacacttat
    16201 ttattttacc actgaatttc tctagactaa agaaaggaat cctttaattt agttgacaaa
    16261 taaacgattt atgatttact tacataaaaa acattgttat ttgaaagaag gaacatcatg
    16321 aatgatttaa aggaaaatat gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac
    16381 atgcaaaaag agatcgtaaa taacggtaca ctctctccac acactgcagc atctattctg
    16441 acaaacaatg atttgttagt aagaacattg aaacgtaccg ctgccttgaa agttttagtg
    16501 aacgtagaca tctccacttt cccttatttg agtcaacaaa ctgatatggg tggaatgact
    16561 gcttctgtac ctccagaatt cactgattta cttttgaagg attctttgaa agatacagat
    16621 aatatgttga ctatcacaaa atacaatcca agtgcttttt ttggtacttc tcttgatctt
    16681 caacttcgcc ggcgtggaat cgaaactatc attttaagcg gtgtagcgac aacaaacggt
    16741 gtatatgcta ctgcattaga tgcctttcaa cacgggtacc atatcgtgct tgctgaagat
    16801 gcttgcagtg atcgtgacaa agaatcccat caattgttca taaaaaagat atttccgaaa
    16861 actgcacgag tacgctcaac gaaacaaata atcgaagcta tccaacaaag caaataagta
    16921 ctggcacacg tgattttcat ttacttcttt ttgatttcat gggaaaataa ccacaggatt
    16981 tagataggag tatgaacatg gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt
    17041 ccggcgtttc aatcacaact gtctccttga tattaaatgg aaatacggag aaattcagtc
    17101 cgaaaactgt tcagaaagtt ttagctgcca aagaagaatt aggctatcag cctgactatt
    17161 ttgctagaca aatgattacc aaggaaacga aaacaattgg tgtactcgtt cctgatatca
    17221 cgaatccatt cttcagtaca ctgatgcgag gaattgagga tatcttgtat aagcagaatt
    17281 tcgtcacgat attatgtaat gctgattctg atcatcaaaa agaaatcgaa tatctagccg
    17341 agctcacacg tcgaggtgtg gatggattca ttatcgcgac ttctgctgtc tctacagatg
    17401 caatcaacga aaatttgaaa aaacaaggcc ggcccttcat tgttttggat cagaaaaaat
    17461 ctgaaggatt tagtgatgcc gtacgaacag acgattttcg cggtggttac ctagcgggca
    17521 tgcacttgct tagtctagga catcaaacga tcgcccttgt ctatcctgaa aatccaccgg
    17581 aaaatgtcca cgctcggatt gaggggttta aatctgctct tgatgtttac caaatccctc
    17641 acgaccaact gatcctctta cctacacagt tctcaaaaca aggtggctac cagatcacag
    17701 cagaacttct tgattctgct gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg
    17761 gattatatcg cggattagag gaagctggaa aaagcatccc tgaagactac agcattattg
    17821 gctatgataa tatagatatg tgcgagtata tcaagccgaa actgacaacg atcgcacaac
    17881 caatcttcga attgggacaa acatctgcca agttattgtt agatcggatc caattcccag
    17941 aaaaagaatg ggaagaaaaa agattacctg tacgattaga aaaaagattt tcaactgcac
    18001 ccttgaaata gctttcaagg tgtgctatat ttttatcgaa ctattaaaac gttttactaa
    18061 aacgttttaa aaaataattt tcccatcaaa ataaatgaga tagttggagg tttgtaaaat
    18121 gaacacggtt acagtaattg gaagtattaa tatggacact acccttcgtt taaccaacat
    18181 gcccaagcca ggcgaaacga tgcatgcgca tgagattttt cacgctggcg gaggtaaagg
    18241 tgccaaccaa gcagtggcag ccaaacgttc aggcgcacgg acatctttta ttggaggcgt
    18301 tggggctgac agcgaaggac aacagttatt agatctactg acaaaagaaa acatcgatac
    18361 atctgggata gctgaaatcc aaggcgccac aaccggacaa gcaatgatta tggtagacgc
    18421 agcaggagaa aatagtatct tgatccatgc aggggcaaat aatgctttcc atgaacaaga
    18481 agtcctaaaa aacaagcaac tgattacaaa cagtgacttt attattgctc agtttgaaag
    18541 tagcttagat gcgacgatcc tagctttttc tattgcaaaa gacgcaggaa aaacaacgat
    18601 cctgaatcct gcaccagctc gtgaaacgat tcctactgaa ctacttgaaa agacagatat
    18661 cattatacca aatgaaacag aaacagaaat cattacagga atcagagtga cagatcataa
    18721 cagtttggtc gcagcagccg aaaaactcca tgaattaggt attggaacag taatcattac
    18781 actaggcagt gccggcgctt tctatcatac agaaaaagaa cacggtatcg tccctgcttt
    18841 taaagtggat gccgttgaca caacagccgc aggtgataca tttattggcg cattaagcag
    18901 cacacttcag ccagacctta gtaacttgaa agaagcgatc ctttatggaa atctagcctc
    18961 ttctgttgct gtgcagtctt atggtgcgca accttctatc ccttaccgcg aagctttaga
    19021 acaatcagca gtctaaaatt aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt
    19081 aatcaattca gatatttcac gagtaatcgc acaaatgggg cactttgata aattaagtat
    19141 cggggatgca ggcatgcctg tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa
    19201 cggtcttcca agttttttat ctgttttaga aaacgtttta gaagaacttg aggtacaatc
    19261 gattgaattg gcggaagaaa taaagacaat gaacccggaa atattggaaa aaatcaaatt
    19321 attgttacca gatacaccga ttagctttat cccacatgaa gaaatgaaaa aagagttgaa
    19381 taactgtcat gcatttattc gtacaggcga aatgacacct tacgccaata ttatattagt
    19441 gagtggagtg gtcttctaat ggaaacgaca atgaatcctg aacagatggt actgtccgag
    19501 cagattcata tgctgattga acagttggga ggaaaagaca atattgattc tgtccttcca
    19561 tgtgcgactc ggcttcgtat caaattgaaa aaacagccag tagccaatgc agaaaaatta
    19621 aaacagcaaa tcccttccat taagtatttc attttactcg atagatatgc agaaataatc
    19681 atggatcagg gattggctcc tgcttacaag gaattgacgc gtcaaatcga tcattcagct
    19741 ccgaaagaaa agctaaatcc gatcgaaaaa ctatttggtt tgatatcgga aatctttacg
    19801 ccgatcattc cgctgtttgc cggttctggt atattaaaag gtctggttgt tttagcgact
    19861 acgattggct ggcttagtga aacaagcgga acttatcata ttttgtctgc tgcgtcgaat
    19921 gccgtctttt attttctgcc aatctttctt gctttttctg ctgccaaaac gtttaaagtg
    19981 aatggatata ttgcggctgt cattgctgca gcactgattg aaccgaatct gacctcttta
    20041 ctttcagaca gtggcaaagc agctactgat tttctgggga ttcctgtagt actgatgcag
    20101 tatacgtcta ctgtcatgcc ggcgatttta ggtattttct tttattcttt tgttgaaaaa
    20161 ttcctcaaaa aatatattcg cgaaaatatg caattagtat ttgtaccttt actttcatta
    20221 gttatcacgg ttcctttgac gttaatggta ttcgggcctg cgggtgttta ccttggcgaa
    20281 ggtttagctg ccgcaattac ttggatgatg gatattaacg gaccgatcag tggcgcaatc
    20341 atcgctggtg gttggaatat tttggtaata tttggtatcc aatgggctgt aaaccctgtc
    20401 atgatcagta atatctctgc ttatgggttc gaccgaatcg ttccgttgac aggagcagcc
    20461 aattttggaa tggctggtgc agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc
    20521 cgttcgatct ccggttctgc ttttgcttcg attttacttg cgggagtgac cgaaccaact
    20581 gtttacggaa ttgcgatccc attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct
    20641 gctggtggag ctgtaatggg atttgcccaa gtgaaagcga tcgcttttgt atttggtagt
    20701 ctgaccacat tgcctgcatt catttccggt acttttttct ggtacttagc aggactggct
    20761 gttagtctag tcgtagcaat gatcactact ctcgttagcg ggtttgatga agacctaatg
    20821 tcttatgaat agttaatgaa aaagactgaa gtatgagatg tttatcacat atttcagtct
    20881 ttttcttttt aggatcatta tttttataaa tttttgtgat actttccaaa ctttggtata
    20941 atttacatat gcataaccat ttttatcccc acttgtttat ccccaaacaa aggtggtcta
    21001 tgcaataaaa tgaacgtcaa agaaaaacga atgaatgctc gttgcttatt tacaaattgt
    21061 tttatttaga atacacagac agatttatga aaagaaaaag cttttcgaca cgattatctg
    21121 ttccaaaaaa tgtctaatga atgctgaata agacagaaaa tcaatgaaag gaaaaggact
    21181 agtttattat gaaaaataaa aaaacgatac gtttgctagc tgctacttgg ctgattgctc
    21241 cgtctgtttt gtcgaccaat accgtatttg ccgtagataa tcaaatagct gatcaaacag
    21301 tcacaacaga tacgactaca tccagctcag ctactagtga aacaacggat tcttttacag
    21361 aagacccaac tggaacaact aactcaacaa caggatcgac cgagccaact gaaattgatc
    21421 cggctgatcc atcagaaaat gaaaagccag ccgagacata ttcccttaca attgctcctt
    21481 ctttgattgg taagatccaa ctgactgctt cgacagcgga ttcccctgaa cagcaacctt
    21541 tggatattac ggaaagtgga acggtagaag gtttattagc ggaaacgaag gtaacttaca
    21601 ctattacgcc agctgaaggt gaacaaattg cttcagtcgc tgttaatggt gccaaagaag
    21661 taaattatga tgcaaagacc tttacagtag gtacgaacaa tttatctatt actgcagaat
    21721 ttaaagctgg accggatact tcaacagaga aaccaactga tcctagtgaa gaagtcccta
    21781 gtacaccaga tccggaaacg ccaaacaaac cagatgtggg taataacaac aataataatg
    21841 gttcaaataa ttcaaacaat aataacaata attcgaacag cgggaattct tcgaatcaaa
    21901 caaacccatc tggatcgaat aacaatggaa atactcagag agaaccaaac catccaaaca
    21961 atggttcatc gaatggcgga acgaacaatg cttcatctcg tccaaataca gcaaatcaaa
    22021 gcggtataga gaatccatct tctgcttctt ctgattttgt tgtaaaatca ccaattgatg
    22081 ctgtattgcc tacaaataca acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc
    22141 atcttggaaa accatatgta tggggagcaa aaggtccaaa tacttttgac tgttctggtt
    22201 tgacttatta cgtatatatg aaagcaaccg gtcattatat cggtggctgg acaggcgaac
    22261 agcaatatgc aggtacgcag attccagtaa gtcaggccca acctggcgac ctcgtcttct
    22321 ggggaccttc ttccggtgtt actcaccatg taggtatcta tattgggaac ggacaattta
    22381 tccatgcgcc tcaaccagga gataaagttc gagtaacttc tatttcagat ttcacgcctg
    22441 attttgctgt ccgagtaaac ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg
    22501 gctcatctat tttagatgga ttagatggct cgttccattt cagtcaaaac caaacaactg
    22561 atcagttctt gaaaaagatt gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct
    22621 acgcatcagt catgatggct caagctatcc ttgaaagtgg atctggaaac agcttattat
    22681 caagcgaacc aaatcataac ctttttggta tcaaaggatc atataaagga agcagcgtga
    22741 ccttcaatac acttgaacaa gatagttccg gacaaagcta ccaaatccgc gcacaattcc
    22801 gtaaatatcc ttcttacaag gaaagtttgg aagactatgc cgacttgatc aaaaatggac
    22861 tgactggcaa tcctgatttc tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag
    22921 aagcaaccaa atatttggaa ggacgttatg ctacagatcg ccaatattca caaaaattga
    22981 acgcgatcat cgaagcgtac gacctgacaa aatatgatga accgaaaaaa gaagcagcaa
    23041 caactgaaga agcaaacgct gaaatcctca atatgtctaa gcgattcgat gtgccaatca
    23101 aatggacaac agatggcgtg ccaatcacag gattagaatt ttcaacgata aaacgcgtca
    23161 acaacgcttc ttccatcttc cgtgtcgctt caatctggga tttgtggaac aattttacgg
    23221 caagacagat accagaaaca actactcgta cagttaaagc tgcaactgtt cctgtactaa
    23281 ccttgttaag tttagacaaa tcattctggc ttgatctgcc tttaaagaaa tagaataaat
    23341 taaaaagggg atgtgacact gtcacactcc ctttttaatt tattctattt ctttatattt
    23401 attttgtatt ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac atctccaaaa
    23461 ctttcaagtc gctccgctcc tggcagttct tgagtctcaa atgtgatacc gccatgattt
    23521 actaatttat tcccgcgcat ttccggtcca tcttctccaa aatttgctgt aaaaatcacg
    23581 attcctgaag catctgtttt tacttggatc gatacttttt catctgggct tgtcaattcc
    23641 gcagcattta gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat gccgcccact
    23701 atcattgatt ggcgaaaatc actggcaaag acctcaccta attttttagg acgttggaag
    23761 tcaaatgctg tgccacttac tgaaactact tctcctgtag gcgttgtatc tttattgagc
    23821 gtagcaaata atcggctatt caaccaaagt atatgttcgt cgattgactg tgctggatca
    23881 ccggttaaat tgaaatagac atgatttgta ggattgaata ctgttttgcg gtcactgatc
    23941 ccttggaccg tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac ttctacatct
    24001 aaagttccag gaaatccgtt ttccatatct gggctagtct gatagaaaat gaccgatgct
    24061 tcgttttcac cattgatgac tgtataatgc cactgcttgg cttcaaagct aggtgcacca
    24121 ccatgtaaac aatgaccagt gtccgggtct gtttttactt cgaacgtttc atcatctaat
    24181 ttgaatgtgc cattcgtaat ccgtccagcc atacgtccga ttgaagcacc gatataaggg
    24241 tctttttcta gatattcttt tgcttcatca aaacctaaga tcaactctcg tttttcatta
    24301 tcgattattg tttgaaaatg aacaacccgt gccccaagat cactgacaga caaagatgtc
    24361 ccattttgat tcgtgatcgt aatcaagtga tagttttcgc caaaattttg ttcattgatt
    24421 tccatgttcc ttgcaccctc ttttctttca tttgcactga ttccagtata catggaaacc
    24481 gttttcttaa cagcttattc cgtgagtaac gagaaaaaat ttactatatt ctaataaaag
    24541 accgtgaaaa aaaggttgtg acaaaaatat tgtcacaacc tctttttccg aacaaacggt
    24601 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg aggagctatt
    24661 tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaatct ctttttcaat
    24721 acgattccag tatataacca tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat
    24781 atcgtaaacg gaacccaaat atctttgtat ggattttgca tgaatagacc aaacatttgt
    24841 aatccgaata agacatggac tacacctaag attcctggtg ctaagaataa cacgcctact
    24901 tctctacgga tcgattgttt aagcaacccg cgtcgtgttc ctatcttttc aagcatttca
    24961 taacgagcaa tgtcactctt gcttcctgac aagatcttga acatcaaaca gctagcaagc
    25021 atggtcaaga aagctaaacc taggaagaat cccataaatt caaaaccaga gaatagacca
    25081 ttatacactt catacatttc atatttctgg ttgctgtact ctactttttg caaagatgga
    25141 ttgttcattt tattttgagc aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct
    25201 tttacctgaa ctagctgtaa agtggattgt ggcaagttca gttgatcaaa ttctgattga
    25261 ctgactacct tgatttcttt ttcttgctgt tccggcaatt catagcttcg aagttcctct
    25321 tgtacagtca gttcattcat ttttttagca ggaacttttt ctttcgtgat cttcggcggt
    25381 gttccatctt catgttttat catgatcaat ggttgagccg caaattcact tgcattatag
    25441 taaatcgttc ccgcatcttc tttttgagta taagaagcag aaagtgttgg tgaaagttct
    25501 ttgatttttt gctgatcttc tgcccgagga ttattcatca ctagatcata tccagaattg
    25561 ttttctgtca agtctgcaat ttcattttta aaacctaatc ctaccgtcaa agcgcccagt
    25621 gccaaggcaa acagcatagc gaccattgag agcatttgtg tatattcacg aatacgaaag
    25681 tttaactgtg acaatgtgaa attgttcaat tttttcaatg aaatctgttc tgatttcttt
    25741 agtaatgata aaaagaaaat gatcacagaa tggaaaatga aataagttcc taatacgatc
    25801 gtaactaagg ctacccctat accaaagact tgataggtcc ccacgtgttt catcatatag
    25861 taaccgcagc ctagaaaagc aattcccagt actacttcaa ggaaaaacaa gaatttatgc
    25921 tgcttgattc ttgttggtgt ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca
    25981 atcgaaaaag cattgacaac agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg
    26041 atcaacaacc cgtgaatact aaaaggtgta aaatgcgaaa tattgagatc taactgatcc
    26101 actaataatc ggtggacgat acttgtcaat cctacaccga cagctgaacc cactaaagta
    26161 gctgcgatcc ctactaagaa tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt
    26221 gctcccagca tcataaacat tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt
    26281 gcatataaaa tataaacgaa tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg
    26341 ataactgtag ccgcaacgat cgtattactt ttcaaaaaag cttcgttgct tgccattgat
    26401 tcaaacatgt aaaaaatagc agatgccata actaatcctg agaataacac caagtagtct
    26461 cttaaacggc tcttgatccc agtaagtgat aatttccaaa gcatagtcaa tctcccccta
    26521 ctgttccaag tttccaagaa tagatagtat ttctcgatag aaggaatcac ggctttgatc
    26581 tccacgtttc acttcttgat ggataccgcc atccttgatg aataaaatac gttggcaata
    26641 acttgcagaa aacggatcat gcgtcacaag aagaatcgat acatgatctt ttgtattcaa
    26701 ttcgtccatc gtatctaaaa gatcccgggc acttttggaa tccagcgcac ctgtaggctc
    26761 atcccctaat agaatcgttg gttgtgtgat caatgcacgt gcggcggcga cccgttgttt
    26821 ttgtccaccg gaaatctcag aaggatagct ttccaaaata tgagagatgg ataaacgttc
    26881 tgcaattttt tggacacgtt gcttgatttc ttttggtctg acgccttgta aagaaagagg
    26941 aacagcgata ttttcttgtg ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa
    27001 gccaatctca ttcgcacgaa aatctgctag ttgatttcct tttaatgtgg tcacatcttt
    27061 ttgattgatt cggatatgcc catctgttgg tttatctaat gtggataaaa tattcaaaag
    27121 tgtcgactta ccagaaccag aagcgcccat gatcccaata aattcgcctt tttctacatc
    27181 gaagctgatg ccgcttaatg cttgcgtctt tttctcattg ttttttccgt atacttttgt
    27241 tacattctgt acttcgacta ttttgttcat ttctttccct accctttaat tagtttttcc
    27301 agtgctgttt tcggcacgtc ttttttttcg acttcttcat acgaagtgac acctttattt
    27361 ttgttccagg tgatttttaa atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg
    27421 aagtccagtt ctttttcctt accctctttc gtatagccag gcaggatata tctatagttg
    27481 actgtttctt gtccagagtc tgattttgaa actgtttttg taccatctgt cgtaatttgt
    27541 acatagtatt cttctccacc cataactacg taatctgcga ttttcacccc tgcaagtacc
    27601 acaacgatga aaacaagtag accgatcaga agttttttca ctttttcccc ttctctctta
    27661 ctttatatac tgattataga aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct
    27721 tctttcaatt ttgtcatttt ctttaaaaca actaaaaaaa gagttcaaga caataatccg
    27781 tcctaaactc cttcttccta tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc
    27841 tgtattgata acaggttgcg ttcctttgtc tgtaatgatt gagacaagta aattattgat
    27901 caattggact ttccgatcat cggtgaaatt gatttcttgt ccatcttcga tttgctctaa
    27961 ggccatttga gtgattgtga cagctccttc aacaatggtt tgtcgagcag acaaaatggc
    28021 ttttgcttgt tgacgttgga gcatggcact agcgatttct gttgcatagg ctaaatggtt
    28081 caagcgtgtt tcgattactt cgacacctgc tactgctaaa cgctcttgca gctctttttg
    28141 caactcgtca gacactgccg tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt
    28201 atcatacggg tattggctag cgatatgccg aatggctgtc tcactttgga tttctacaaa
    28261 atcttgataa tacgctacat cgaataatgc ctttgctgta tcaacgactt taaagactac
    28321 tactgctgag atttcgatgg ggtttccatc caaatcattg acttttaaaa cagaactatt
    28381 gaaattccga actttcaacg aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa
    28441 gccattttct cgaatcgtac ctaaatattg tccaaaaaac aaaattactt ttgcttgatt
    28501 aggactgact acagttgccg aacttaacaa caataacgtg atcaaccaaa gaaaaatact
    28561 taaaaaaagt gcccaaactt tcgcttgcca catacctaaa tagaagaaaa acaatccaac
    28621 taaagctaat acgaccaatc cgacaatccc tgcataacca ttgatataaa atgctctttt
    28681 ttctctcatg ctacttcctg cgctccttta tccttcaata aatatctaat gttacttttt
    28741 taattttttt ttcgaatacg cgccattaca ccttctagtt ctaactcttt gtcttcagtg
    28801 atgtaccaac cgcttgcctg tgtccctccg gctgcctgcg gtaaataatc caattttgtt
    28861 cgaacccagt gcttttcttc tgtaaaatag tcttctacca agacctctag atccatccct
    28921 gctgaaagag gcaaatcttc ccgaacagat ttaaagataa atcgttcctt cttcccatca
    28981 tacttcaata ttcctgtatc gataatcggt aaagaataat aatccagctc tttcttcacc
    29041 gtcaataaat gtttgaaaac ttcatggtac acttgctcta attggcgacc ttctgaattg
    29101 ttcgggtaat taaccatcac tttttctttg tctggatcat agaaggtttg gcttagttga
    29161 gtgatataag tatgtaatct ttccatttct gtttttaact tttctctttg accttcttct
    29221 accttcatat actctttcac cctgttttct ttttagatat tttctttctt attttactat
    29281 agttatgtgc gttaagcgat gaaaaacagc aaagattgga aggatctttg ctgttttttc
    29341 ttaaaatatc cagttgagta caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa
    29401 tattgatcga tgtgtcttta gcagtgatct ttagcttttt ctgtccttgt tctaacaaag
    29461 tatttgttat tggctggtag tctttttcaa tataagattc accagacgtt tggatagtat
    29521 agccactgtt ttccatctca tgcaaagata aattgccacc gtttaggatg acttttcctt
    29581 ctttgctgat caaactgtct tctagatgga agttgctgtt ttcacctgtg acgatcaagt
    29641 tatccacatt ggcctcatct aatcgaagtg agcctaattc atctttcgca gttattgttc
    29701 ctcctgtaaa ttcattgaac gatacccagc catcatgtgt cttagtattc acttttttga
    29761 ttgatacttc gttgatatca attgcactat tattcccttc gattgtcaat tcagataatt
    29821 catgatctgc agggacaaca atagtgattt gtgcacttgt gtcttctaaa ctaatgattg
    29881 cacctgttga ttttttgact gcattcacag tcaacacgcc atttttctcc tttatttctg
    29941 gcttttctgt cttatcataa gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa
    30001 ctgtttgatt ttctgcatgg atgacgattt tgtttgcatc cttgattttt tttgtctcag
    30061 taaccatatc gatcaaccga ggaccattat cccaagtcag actcttttga gcgcctaatg
    30121 tataagttgc acctgctagg aggccaccag taagcatcag accaataccg gtaatcaaaa
    30181 aatgtttcaa tctcaattta tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt
    30241 ttgtaataaa acgtcgtccc atccatttaa caaagttcat catgacataa aaaagccctt
    30301 ttgttccttt cagcaacagt ggcgaaataa atactgcgct tcctgtggcg aacagaccga
    30361 caccgataaa gaataatcca ctcagaatcg attgtccaaa cacactgatt cctgagatga
    30421 tcatgaatag gccaacacct aagatactga tacataaaac atagatacta aaaatgagcg
    30481 ctagtaaagc aaccaatagt cccccaataa ccaatataaa ccccagtgct agtggaatga
    30541 ggacaggaga agcaaataaa ccgataatga ttaaccaaat caggcgaatt tgtctttttg
    30601 gatttttcac taactcgttt tctgcttgtt cttcttctga aaagtaactg atttttaatt
    30661 ttaaggcaaa ctgctttggt gtaccatatt catcaatgat ctgatctaat gtttttccag
    30721 aatctaaaaa atattcctca taatactgga tcaaatcaaa acgatcgtct tcaggaattt
    30781 cttgaagatg atccacaact tcattcaaat attcattcat ttgtgctctc ctccaataac
    30841 cggtctaaag aatctcgaaa aacaatccat tcatcaataa tttcttctaa atgacgactt
    30901 cccattggtg tcaacgagta atatcgacgg tttctgcctt caaaaggttc atcataggtc
    30961 tcaagatatc cttctttttt caatcttctc aaaaccggat atagtgtaga ttcactgaca
    31021 gagagacttt cttgtacgat ttttgttaag gcatacccat acatatcttc cttgcttaat
    31081 gctgctagca cagtaccatc aagaagaaca gttggaatct ggatgtgcat tacgtcacct
    31141 tctattctca agtgtaatat tttatttcaa tctatattat acaacatata atatagtttc
    31201 aaaatcagtt tgtcaaatac aaaaaaactg actaaaagat ttgatccttt ttagtcagca
    31261 aaaaatggct tctctttgat ttttattata acagtggcat aatccaattt gtccacatcc
    31321 agctcagtgg catgataacg atcattgcag gtatcatgtc agctactggg aacattttca
    31381 cgttgatcat tctaaaaccg gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta
    31441 agatcatgtc tggtgtagtc aatggataaa taaattttgc caataagaac aaaatataaa
    31501 aaataatgaa ttggggaatt gctatcacag aaacgacata ccctaaatta cacgcaaaaa
    31561 tagctgctgt gaaaaaatca aggacagatt tagaaatcaa aatcgtactg tctcctgtca
    31621 tcccagaatc taaacttcca tagatccccg taccacttgc acaaaataga acaatgaccg
    31681 tcactaatgt gctgataaat tcatcgtggg tcataccaag cttttcacta gggaagattt
    31741 tagaaatagg tacttgcatc agcgcagccc ccttatttat cccattacct aaatgaacaa
    31801 tcaaaccaat cgctgttcct atcactaaag caaaaataac agccggcata tttttcatcg
    31861 gcgcaatgga atagattccc atccccatcg aacacacacc aaaaaccatc gtcagctgtg
    31921 ctttaaaatg ttcacttaat ttatttccta ctaatccgcc aattagtccg ccaaacaaaa
    31981 cggaacctac attgatcaaa actccagttg gcatcaaaaa actcctctct ttttctacga
    32041 agcgaaaaaa atcagaaaaa tttctgtaat tattgctttc gctgcaagaa agattgtaac
    32101 agacagagaa gattacacca attcattttt tgtgttaaag tattctttat tggaataagt
    32161 aggaggaata tgatggatat ccataaattc gaagtttttc ttgatttagc cgaaacaatg
    32221 aatttcacca aaactgctga tcgacaattc accacccaag gaaatatttc taaacagatt
    32281 ctttcgttag aaacagaatt agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa
    32341 ttgaccgaag ctggaagctt attgctccca tatgtaaaaa atgtcgtaga acaataccat
    32401 gctatgcaag aagtgcttga aacctatacg aaagacaaca atctttcatt aaatatctta
    32461 acgataccga caatgatcaa ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc
    32521 catcctgaat ttacagtcca gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac
    32581 gccaatgaac atagtgacac gatccacttt gctagaagtt tccaggcccc accaagttcg
    32641 attgagtggt tgcctactga aacagacgat tttgtagcag ttctacctaa aaatcatcct
    32701 ttagctagta aaaagaaatt ggatctaagc gaactgaaaa aggagaactt tttgctcctt
    32761 ggccccacta cgaacttgta tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa
    32821 ccaaaaatct cttataaagg agcaagaatc gacttgatca tcaatatgat tgcaaatgac
    32881 atggggattg ctattgttat ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg
    32941 attctcccta tttctccaac aacagagagt tatcttgcat ttactcgtaa agtcggtgag
    33001 cattctttgg cttcagatac attttgggag tatttaaatc aagaagaaaa ctaagcccta
    33061 cattttctgt ccatctatcc acccaaaaaa agagagcgac gcatgacttt cgctctcttt
    33121 cttgcatcat gtccttgttt caatgacacc tctaacattt ctcgtagttc aataagaaag
    33181 cagcgatttt tatgccaaaa gaaccgtaag aacatttccc taatcacaaa aaaacgacta
    33241 aaaaccccta aaaaccttca atagaatttt taggggtttt attctttttc ctattgttta
    33301 cttaaccgta tcattgctgt cgttcctact ccttcgatac tgctataatc aatacgtcca
    33361 tcatgcagtt ctagaatttt tttaaccagt gaaagtccta gcccattccc agaaatttga
    33421 cgacttgtat ctccttgata aaatttttca aacattcgat ctctggtctc ttcattcatt
    33481 cctacacctg aatcggtcac ttccagtaca atctctgtgg ctgtctcaaa tagtttgata
    33541 tgaatctgtc cattgatttg attgtacttt atggcgttat ccatgatatt cagccacact
    33601 tggtacaaaa actcttcatt gcctgtgtaa tttactgcag caagttcgat atccaattct
    33661 agctgttctt tctcccattt aggctgaaaa aaaacgatta cttctctgat ttgttcatct
    33721 aatcgatact ctttcttttc cagttggata cgctggtttt ccagtttgtt caattttaag
    33781 atgttctctg ttaattgtga aagctgcgtg atactttcga taatccgata taaaaatatt
    33841 tggcgttctt cgtcagagag atttggggct tgtaacaatt gaacatatcc ttggattgtt
    33901 gctagtggtg ttttgaattc atgggataca ctggacacaa agtcattcct cagtgtctcg
    33961 atactattga gttcttggac cataacgttg aaatctttat acagttgctg tacttctgct
    34021 actttctgct gttcatccat tcgaatcgaa aaatctcccg ttgctaccag actcatattt
    34081 gtccctagcg cactgattgg atgcaggata cgccgcccaa caaaaatcga tattcctgta
    34141 cccacgacca tactgaaccc cgcaaagacg aataacgggt ataacgttgg ttttccagcc
    34201 gattcagcag gcgagataga atgtcgagac aataagaaaa taaaaccgag aaaacacagc
    34261 agtgtcacta agatcgaaat gaagacaata ctagcaaaat agatccataa ttgtgaaaat
    34321 agcttttttt tcatctttaa ataaccgcct tatagcctag tccccgtact gtgatgattt
    34381 gaaaatcttc cgtcttttca aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc
    34441 gttcttctgt attcgtatct aatccccaaa tatcatccat caactgttgg cgagtgaaga
    34501 tcttgttggg ataagacagt aatttataca gaagcagaaa ttctttttga ggcagaatca
    34561 acgaatgctg atcttgtaac actgtataag catctagctc taattttgtt tgtccgatta
    34621 tcaagctact gctgtaattg atctttgaac gtcgaagcaa tgcttccaca cgtaggatca
    34681 tctcattgac atcgatgggt tttgtcatat aatcatctgc tcctaatttg aacccgcgtt
    34741 tcttatcctc aaaatcggcc tttgccgtaa tcattagaac aggtgtgtca aatctggtgc
    34801 tacgtaatga gtctagcaag tcatagccat ccattttcgg catcatgata tctgtaatga
    34861 ttaattctat acgtgtcgct tctagttttt tcagcgcatc ctctccattc aatgcggtga
    34921 tgactgaaaa gcccactcgt tccaacacag aacggtaaag caactgtaat tgcggatcat
    34981 cttctactac taaaatacga ttcattgatc tccctctgtt ttttgaaaaa tttctagatt
    35041 ttttcttgta gttcatcttc agttaacatt tatttcgtat actacgatca ttgttctgat
    35101 atacttatta gtataccgaa tatttcaata cgggagaagg gtgattatgc ttcaattaaa
    35161 agatataaaa aaatattata aagtcggcga aaccaccacc aaagcattgg atggtgtgtc
    35221 tgtcgctttc agacaaaaag aattcgtagc gattttaggt cctagtggtt ctggtaaaac
    35281 gactatgctg aatgtgattg gtggactaga taattatgat tccggtgata tggtaatcaa
    35341 cggaaagtct actaaagact ttaaggacag tgactgggat gcctaccgaa acaactctat
    35401 cggtttcatt tttcaaagtt acaacttgat tggtcatcta ggcattattg aaaacgttga
    35461 acttgggatg acattaagtg gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc
    35521 attgcaccga gtcggcttga caaatcatat gcataaaaag ccaaatcagt tatctggtgg
    35581 acaaatgcag cgagtggcca tcgctcgagc actagccaat gacccagata tccttttgtg
    35641 cgatgaacca accggagcgt tagatacaga aacaagtatc cagatcatgg aattgatcca
    35701 agagttatcg aaagaaaaat tagtgatcat ggtcacacat aatcctgaac tggcccatca
    35761 atacgcagat cgaatcattg aattttctga tggtaagatc ttaacagatt cccatcctca
    35821 catcgaacga ccaaaagatg atcaatttaa cttacgcaga acaaagatga gtttttggac
    35881 agcgttaaag ctttccttca acaatatccg aacaaaaaaa ggacgtactt tcttgacctc
    35941 ctttgcttcc agtattggga tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt
    36001 ccaaaaacaa atcgataaca cgcaagctga aacgatggcg aaatttccga ttactatttc
    36061 taaggtaacg actaaccaga ctcgtgacga tgccggattg ggtgcaagca aagcggatta
    36121 tccagacagc aaaacgatta ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa
    36181 taagattgat caaacatacg tcgattacgt aacagacatc gatcctaact taagtaataa
    36241 tatcggattt acccgtacta caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca
    36301 gcctgtcagc ttctcgaatc aaaatcctga tacggaatct ctttctttat ctagtacgat
    36361 gtcagcaatg acaggtgtgg gagtgtcaag tttccctaca cagcttgaca ctagcaaaga
    36421 aaacttttta aaagacaatt attctttact tgccggaagt tatccagcct cagcaacaga
    36481 tgtcgtgctg atcgtagacg gcaataataa tactaatatc aacgcattga agaatcttgg
    36541 atttgatgtg aaagaagacg aaaaacttga ttttgatgat atcgttggta caacattcaa
    36601 attagtaaac aacaatactt actatacaaa acttccaact gggaatttta tccctaatac
    36661 agattatgat gcgatgtatc agaatgcttc tgatgaattg aagatctcgg ggatcctacg
    36721 cgtgaaatct tcttctacaa tgaatctctt atctccagga atcgcttata gcgatcaatt
    36781 aacgactcaa atcgtcaacg aaaacaaaga atcagaaatc gtcaaagctc aaagagacag
    36841 tggtgtaaat gtcttaacta ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag
    36901 ttatttaggt ggagacagct tgccttcaag tatcatgatt tatccgaaca attttgaaga
    36961 caaagaaaag atcttggatt atctagatga ttacaataaa ggcaaatcag atgaagataa
    37021 aatcatttat actgatttag ctggaacgat gactgaactg actggcggat tgatggatgc
    37081 tatcacttat gtccttattg cttttgcagg gatctcttta gtaacaagta tgatcatgat
    37141 cagtatcatt acttatacat ctgtgattga acggacaaaa gaaatcggtg tactaaaagc
    37201 tttaggtgca cgtaaaaaag acatcactcg cgtatttgac gcagaaacgt gtattttagg
    37261 tatttcttct gggatattag gtgtgtttat tgcatggcta gctacgttcc ctatcaatag
    37321 catcttatac aacatgacgg atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat
    37381 tattctagtg atcgtctcta ctatattgac gatgcttggc ggtcatcttc ctgcacgaat
    37441 ggcagcgaaa aaagacgccg ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat
    37501 ttagatttga gtcataagag cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat
    37561 atgttccttg tttcgcgctg ttttttttaa ttatttttta taattctggt atacttttct
    37621 ttcctttgta ccacaactct tctggcgtat acctgtcttt tccatttatg aatactgttc
    37681 catctgcttc aatcgttact tcagtcccaa tccacatatt acgagaaagt gcgagaagat
    37741 tttttctttt tcctcttcct ttttttccaa attgcaaggc atcggttatc tcttctgtcc
    37801 aatgagattt cacgtatttt acttctccgt tttcaaatgt aactttcaaa atattcgcta
    37861 tattttccac ttttactgct ttaggaaatg actcctccat ttttttcact ttccttcctc
    37921 caacactaag tatgcgcttt cttttagtga taagtatatc atagtttatt ctattatcaa
    37981 aaaaacgcat ccattccatt gtagaatact ttaccgaaca agttgaattg ttttcctctt
    38041 tttcaccttt tactatagat aggtaaagac aactaaggag gaatctctca tgacagatca
    38101 accgtatgat ttaagaaaag tcatcgaaga actaaaaaca cttccaggtc aatatcacga
    38161 aacagatatt gaaatcgatc ctgaagcaga attatctggt gtttaccgtt atatcggtgc
    38221 tggcggtaca gtaaaacgtc cgactcaaga aggaccagct atgactttca acaatatcaa
    38281 aggtttcccc ggcacacgtg tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga
    38341 aattttgcac catgattatc gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc
    38401 aatcaaacct gtgaaagtag acaaagcaga cgctccagca caagaagtcg ttcatctagc
    38461 taccgatgca gacttcgata ttcgcaacat tttgcctgca cctacaaata ctcctgaaga
    38521 tgctggtcca tacatcacaa tgggtgtagt cttaggatca gatccagaaa aaacaatgag
    38581 cgatgtaacg atccatcgta tggttctaga agataaagat acgatcggta tgtacatcat
    38641 gcctggcgga cgccacattg gtcacttcca aaaacaattc gaagccttga ataaaccaat
    38701 gcctatcact atcaatattg gtttagatcc agcaattgcc attggcacga ctttcgaacc
    38761 acctaccact ccacttggct acaatgaact atggtgtgca ggagctttgc gtaacgaacc
    38821 tattcaaatc gttgatggta tagcagtcga tgaagtgggt attgctcgtg ctgaattcat
    38881 cattgaagca gaaatcatgc caaatgaaac aatgcaagaa gatatcaaca cgaactcagg
    38941 aaaagcaatg ccagaatttc ctggctatac tggggatgct aatccagctg tacaagtagt
    39001 gaaagtcaaa gcagttactc accgaaaaga caatccaatc atgcaaacaa cgattggtcc
    39061 cagtgaagaa cacgtatcta tggccggtat tcctacagaa gcaagtattc ttgccttggt
    39121 cgataaagct attccaggaa aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa
    39181 attgatgacc atcatgcaaa tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca
    39241 agcagctatt cttgctttat cagccttcaa agaattgaag acagtcattt tagtcgatga
    39301 agatgttgat attttcgaca tgaacgatgt agtttggact ttcaacactc gtttccaagg
    39361 agataaagat atcatcgtga tcccaggaat gaggaaccat ccattggacc catcagaatt
    39421 accagaatat aatcctggct ctattcgttt ccgtgggata agtgcaaaaa ctatctttga
    39481 cggtacagtt cctttcgata tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga
    39541 agattgggaa aaatatttga aataatgaat gatctgcaac tgttttgaca tcataaaaag
    39601 gagtgcggaa aatggaacaa ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg
    39661 gcgcttcagg caccatttat gccatcaacc tagtgaagaa actaaaagaa tatccgatga
    39721 tagaaacaca cggaatcatc agtccatggg caaaacagaa cttaaagctg gaatccgact
    39781 tatcactaac tgaattcgaa agcctgtttg attaccatta ttcaaacaaa gatttaggtg
    39841 ctgctgtcgc cagcggatca tttttgaccg atggaatgat catcgtaccg gcaagtatga
    39901 aaacagtcgc aggtatctct gttggtttag gcgacaacct gattgcacgt gctgctgatg
    39961 taacaatgaa ggagcaacga aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca
    40021 ttcacttgga aaacttgact aaattagcac gactaggtgt gcaaatcata ccaccaattc
    40081 ctgcttttta taatcagcct caaacgatcc aagatcttgt taatcaccat acaatgaaac
    40141 ttttggatca atttgaaatt gtaaatgagc attcaaaacg ttggcagggt gattaatatg
    40201 gaaaaagaaa cgatagttga agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa
    40261 gtaatcggta aagacttatt gatcacttta acaggcggag atacaccaca tatcggaaca
    40321 gtcactacat tttctcgtga tacagagatg caaactgtac gtttccctag ccacgatggt
    40381 cggttccata aagacgatgt cttagccgaa aaaatagctg tgatcatcaa aactgccgtt
    40441 ccgggaagtt gtacgatcac ttccggtatc catgttgacc atatctccaa tgcacaaatc
    40501 aacgcctctt ttccgatgtc agaagagtta ggttacaaga ttttagattg gttggaaaaa
    40561 atagatttcc atatcaaaga accagtttat tataaagatg gtgaaaaacc attatgaaag
    40621 aagaggttgt gaaatcagcg ttatgacctg agtatgagag aaaaaaatgg aaaaatagct
    40681 tctcatattt tgccgttttt gagctaatac cgagggacag ctgattgaac gccgtttatc
    40741 aagaggttgt gataaagccg cttcgtttcg agtagtaaga aaaattttgt aaacatgggt
    40801 ttccacattt ttgcataaat ttgacttact accgagaaac tggcttttga acaccgttta
    40861 tcaagaggtt gtgacacgtc ttttgtcaca acctctttta tttttgcttc actgtaactg
    40921 ctacaatatc accgatttct tttccgatgt ttttacgaat atcttttcgt agtccaagga
    40981 tgtagcagac tgaaccatct ggatttttca cgcccatatt cacgatactg ccttcatacg
    41041 gcacattgtc aaaagttgct tggactttaa ctcttccagc accgaattct tttcttatat
    41101 catacggaaa aataatatat gccccgccct tgccgacttc ggaagcatag atttttgatt
    41161 caaaagaata aatttccctc attttatttc tcctattttt tacatctagt atagcatagc
    41221 aaaaaaaaga aggaactgtg acaaaagtct tgtcgcagtt ccttttttcg aataaacggt
    41281 gttctcctgc caactcctcg gtcacaagtc acaatagtca acaaacatga gaagctgttt
    41341 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc ttccttaaac
    41401 ggtgttcaaa agcttgcatc tacggcacta agcccaatca gatgaaaaat atggagtgct
    41461 atttttatct gattgttctt actgcttgat gcagagcagc tttttcacaa cctcctacat
    41521 ggtgttcaaa atattattcg ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt
    41581 ggaattcagc agctaaggct ttcgtacgtt gttcttgctc tgctttgctc atttttggat
    41641 tttcagtcat tgcttttacc ataccgtctt gaacataagc ttttgcttca ctttccatcg
    41701 tactttgctg tttcttcact aattcagcta acttggctgc ttggtccgta cttaattcta
    41761 cccatgtctc tgggattttg aatgtattca ctcggctgtc atattcgtga ttgttatcag
    41821 caatattgaa ccagaattta tacatgtcgt ttttgtattc ccaataagta gttttcgtca
    41881 ctttttcagc atcgccgtct gtcttttcta ctttatttgt ttcgttatcc gtaccagttg
    41941 cttttggttt ttcttgtttt tcatttgttc ggtaaagata cactttttct tttccattcc
    42001 ctaaagcttt atacaaaagc atatctgctc cttgggaatc agcactggaa acaatttttt
    42061 ctgtttttgt ttccgttact ttttccattc caaagtgatt ggacaaatta gcgacgatca
    42121 aacttagtga agcgacgaag attagtccga aaatcaaaga taaaaaggtt tgccatgttt
    42181 ttttagcgaa cacgttcgtt aaagcaaaag caagcaagct gacaatcaaa actccaataa
    42241 tcatgcatta tcccccgctt tcattagaac aggttctttt ttcttcaata aaaaggcaat
    42301 aataaagcca agaatcccaa agatgatcgc tacgataaac gcggcatggt aacctgacag
    42361 tgtggcattg attgcctgat ctttataagc taatggcgta ttatccaata cagatttgct
    42421 tgggatattt gcttttgtca cattcgtcaa cacactgatc aaaatagctg taccaatcga
    42481 gcttgcgatt tgacggaatg tattatttac cgctgtccca tgactgatca agttatttgg
    42541 caatgcattc ataccagaag tcgttaccgg catcatgacc atggagatcc caaacatacg
    42601 gatcgcataa aggatcacga tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg
    42661 tgcagttgcc gctgtcaaaa tgaacatccc tgtgatggct aaacgtttcg cgccgtattt
    42721 atcgaaaatc gcacccgtca caggcatcat agcccccatg atcaatgcac ccggcaacaa
    42781 catcaagcca gaatggaatg ctgattctcc acgaatattt tgaatataca atggtacgac
    42841 catttctgcc cctaccatcg ccatatttgt tacaccagct aaaacagctg caatcgtgaa
    42901 tgtagatgat ttgaatacac gaagttccaa gaatggatgt tccaaatgta attgacgcca
    42961 tgagaacaag gcgatgaaaa tcacaccaac aactaagaaa ccaattactt tcaagcttcc
    43021 ccagccgtca tctcctacac tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga
    43081 taaaatagcg gataaaatat caatgctagg atttgacaat ggaataacac ttttcattag
    43141 gaagaatgct aaaaccaaga caagaacaac gattggcagt accataccaa acaagtcacg
    43201 ccaatggtag ttatcaatga tataaccaga taatgtaggg cctaaagcag gagcaagtcc
    43261 aataacgatc cctgacatcc ccattgctga tcctcttttt tctggcggga aaatagatag
    43321 catgatattt tgcaataatg gcatcgatac tcccacaccg caagcttgga tcaaacgacc
    43381 gattagtaat gtactaaaat taggtgctgt aaagcaagta atcgttccga tcaaaaagat
    43441 cgtcatggca gaaagatata aatttcttga actgaattta ttgatcagcc atgctgttat
    43501 tgggatcatg atcccgttga ctagcaagaa accagtcgtt aaccattgca catcagacgc
    43561 tgaaatatcg aaagctttca tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa
    43621 aacggtacaa aatgtaccaa ttaataagac aacaactaaa agtgtccgat tatatggttt
    43681 tccgtaaata tctacgggtt ggttgttact catatttctc cttctttctg aattttttct
    43741 gaaactacta gaatacttta gtcctaaatt cgctatttgt caacctactt gacacataag
    43801 ttaatacgga tataattaag ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg
    43861 cttcccttaa acgtttgttg gaaaatgatc aattggttgt tggcatcagt gaattaagtg
    43921 atatcgtagg tgtgtctcca cgtcaattaa ggtattggga acaaaaggga ttcattcgtt
    43981 cgatcgaagc tgatgcgaat tgtcctagaa aatatcgttt acctacagtc ataaaagtag
    44041 aactgatcaa aaaatattta gatgacggat ttactttgtc aaaagctgca gaaaaggcag
    44101 aaatccgcca aaagaaaatg caccatgtcc gtaaagtttt ttcaaaagca atcaaagata
    44161 tcgaattgat cgacgaacgc tatacgattt tacaaatcgg taacttcaaa gaagagaatg
    44221 ctaagatgta catcattcat gacaacgaga cagacgaatt aacttacaag ctccttcctg
    44281 tcgatgaaaa agctgactac gaacagttgt taaaagaact ctaaacgcca ctaatctcct
    44341 aagtaccaag ctttttaata agtagatgct acataaatac ttgaaaaaag gcctgtttga
    44401 acataagaat atccgaacgg tgatctgcgt ttctttcata tagaaaggaa ttctccatca
    44461 ctgttcggat atttttttat ttttttcggt tactttcttc taatacatct gttagccaat
    44521 ttgcatattc tgtttcccga ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt
    44581 catgtttttc tttttcagta ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt
    44641 tttcttcatg aaggtcacgc tgctgctgaa taatttctac taatgccgga tcattcacgt
    44701 ctttgacgaa atacaatttc agaacaaatt cgtctcgatt aaccggtagt tcattggtgg
    44761 gtgtatgaat ccactctgcc aattgttcct tcccttcttc tgttaattca taaactttct
    44821 tttccaattt ttcacctgtg atctctactt gatgctgaat gattccttgc tcttccattt
    44881 ttgcaagttc aggataaatc tgactatgtt ttgcttgcca aaattctcct atttcatttt
    44941 tgaacgattg attcaattca tatcctgtca tcttcttctt acttaataac cctaacaaaa
    45001 tatatcgaag tgtatttttt cttgccatca gttttccctc cttgacaaat ctttaaatcc
    45061 attctatcat aaatgagtaa tcgaaaacat gtagtttatt acatgtttta atttataagg
    45121 agatgcttaa acatgaaaga atttaaagaa ttggaagatt ttattggaac tcactttatc
    45181 tatacgtatg acaatggttg ggaatatgaa tggtatgcca aaaatgacca tactgttgat
    45241 taccgtatcc atggcggaat ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc
    45301 gttaaattga cggacggcgt attcaaaatc acttggacag agccaacagg gacagacgtg
    45361 gcattggact ttatgccaaa tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg
    45421 gttgaagaac atccagaaat cactgttact ttccaaaacg aacatatcgc agaaatggaa
    45481 gcagctcgtg aaaaatatga aacttatcca aaattacttg ttcctgaatt tgctacgatc
    45541 acttatatgg gtgatgcagg tatagacaat gaagatgtta ttagcgaaga accatacgat
    45601 ggaatgccag atgatatccg tagcggaaaa tacttcgatg aaaattataa acgagtaaaa
    45661 taaggttgtg aaagagaggt tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa
    45721 cggaaaaata gttcctcatg ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
    45781 gaacaccgtt tatcaagagg ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta
    45841 gttagaaaat agcttttcat attttctagg ctagtgggct tattaccgag aagcagccgt
    45901 ttgaacaccg tttatcaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac
    45961 aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg
    46021 attgaacacc gtttatcaaa gattgtaaca actgcatatt cacacagtta aattagtgac
    46081 ttcaaaatga aataatgctc ccagcaaaaa attaggctgg gagcattatt tcgttattct
    46141 gttcatcaaa aatcagcgtt ttgattccat ttcatcacaa gttcctcctc atttactgct
    46201 tcatcttttt gctgtgtttg aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat
    46261 tgattttttt gatagactgt caaagtcagt gttttattgg tttcttttat ttttttcatt
    46321 agttgcgtac cgattctttg ttgccggtat tcccttttca caaagattcc tgcgataaac
    46381 tgatcaatca ccccagcaaa accaataatt tcttgatcta tttctgctaa gtaaatcact
    46441 gcatctggta aagctttcct aacagattct acttgattct cccaataagt tgatggaatg
    46501 aattcatgag aatccaagtt agcatgcagc caaatcgata gaacagattc cagttctttg
    46561 gcagacaatt ttgttgttgt gcggatcatt ttttctctcc ttacttattc attgatttta
    46621 aaataaaaac catttttttg aagctctttt acaagttttc gcccctcaaa aaagtatatc
    46681 ttctcttgtt ttacttttct aataccaaaa gaaacagcaa ctttaggatc atcagctgaa
    46741 acgattcgat aagttccacc aggcaattga tccagcttga ctttcccatg atggtcgctt
    46801 tgtactgaaa acggcacacc atttctagta agtgtttttt tcttatgttt caaaatgaat
    46861 gaactaccat ttaccggttt ccctgacgaa tcactaataa taaatgaaaa atcaagcact
    46921 tttttactta atctatactg atagattagt cgtccgataa acactaacag tatgactaca
    46981 gctattatcg ttcctgcaag aataaaccat tgccgcatgg ttctttcctg atttcctttt
    47041 ccttcttctt tttttaccga ttcgttataa ggaatccgat gtcctgtcac tagcaaacga
    47101 tgcgtgttga tcatatacgg cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa
    47161 atcgttagaa cagacgtgtc atctggcagt accacttgta tatttttaac ttgataagcc
    47221 aatttctccc ccaaaacagt caaaataaac gtgtctccgt tttctaaacg atctaattcg
    47281 gtaaataacc gtcgattagg taaaccgctg tgagcagaga tcactgaatg cgtcgaattt
    47341 ccaccaagcg ggaaagaggt tccttgcaag acgcctgctc cattttcaag gatcctacca
    47401 ttgagccgat cataaagtga aatatttaat tggatcttag gtatattgat acttccgatc
    47461 agccattcct ctaattggct ggatgttgta ctttcatttc tgttcttttc attgaaagga
    47521 tcagtaccag gattgaatcc ttggtcagtt aatcgttggt ttttctcctc cattttcttt
    47581 cgctgcactt caatttcagc tgcggtccgt ttttgtactt gttcgatacg tttttgatca
    47641 ataaaactat ttaaagaatc cacataaaat ggatataaag cgatcagtaa ccctacgacg
    47701 atacatatta ataaaaacaa acgaaacaaa agtcgacgag actgtttttg cttactctgc
    47761 ttcacatgtg tcctcccttg caagtttttt tgttctttct tttattatat gggttcagga
    47821 aaaaaataga aaatgaaagc cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa
    47881 gtacagccca aaaaaatagc tactcatatt ttttgttggg ttgggcttat taccgcagat
    47941 gcaagctttt gaacaccgtt taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa
    48001 taagagcgct agtcagaaaa tagcttctca tattttctag gctagtgagc ttattaccga
    48061 gaagcagctg attgaacacc gtttaccgag aggtcatgaa aaagctactt tgtttcgagt
    48121 attaagaaaa attctgtttc aaaacaagga gatgcaacaa aacttatgtc gcatcctctc
    48181 tccttagaac tttcacttat gtttttcttg taaaattcat aaatattttc atgaattttc
    48241 taatttcttg cctacctctc gcataggata tatgatgaat ttatctaaag tttgcaaagg
    48301 agttcgacaa ttgaaaaaat atggcccatt tttagcaatt tttttcattg ctttggcatt
    48361 tctcatgcct catttattca cacataaact aattttgggt gctgatgccc cattccatta
    48421 caatcggttc tatgatgccg cgcaacaaat taaatatggg gatttccatt attttccttc
    48481 cctttatgga ttccagcaaa gtggcaggat cgtgaatgcc gtttacggcc catttttcgc
    48541 ttatttacaa ggattgatcg tgttgcttgc aggtagctgg tttcattatc agattctttc
    48601 aaatttcatt ctttatctta tttcaggatt ttctatgctg attttactga gatatgtcaa
    48661 tgttcgaaat tgggtaagtg taccagtagc cgttttattc atgagtacgt attctattca
    48721 atactggtct atcaaccaag gattcactag ctgggggact tgtttttatc ctttatgtat
    48781 gatccctatt gctgatttcg tccttcataa aaagttccct gttatcaaag tcgctgtctc
    48841 agtgggactt atgacacaga tccatttact gtcttcgatc atgttagctt tgatgtatat
    48901 ccctttttat ctttattact tttttaacca gactgaaaaa ggaaaagcac tgcttcaact
    48961 gattaagtcg gtcttgcttt attttgttct gacagcaaat atttgggcag gattagctat
    49021 tgtttacagt gggaataaga ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc
    49081 gatagatctg gatggcagtt attggctcca ttaccctaag gcctttccta tattgatatt
    49141 atcaggtttt ttgcttttta ttttctttca acaaaagaac accttattcc aaaaaatcgt
    49201 gttgtacaca agtcttgttt tcttttttct ttctactagc ttgattccat ggacgacatt
    49261 gatcgaaaaa aatgtgcctt ttattagttt gatccagttt ccatttcgat ttttcgtgcc
    49321 atttacgcta ttgcttctac tttaccttgc cctttcactg aaccagtggt ctgaacaaaa
    49381 gtggttccgt cttttaagta tatttggcat tcttattttt tcagcacaga cgatccagaa
    49441 cttataccag catctggaaa agtgggaaaa tgaaacattt gtcagtcgac atacttatct
    49501 attcgatacg ccggaagaag caaggaaaac attcttcagc aaagatatgt ctaccagctt
    49561 gttcgttttc caaaaaacca cacctgacta tctgccaatc tataaggaga caaaagacaa
    49621 taaatacgac cggtataacg aagaaatact agaaaacgaa acaactttta taaaaacaca
    49681 tccaggcggt cttttgacaa tcaagtggaa aaacactggg gacaaaactg ttcatatccc
    49741 tgttatcgtc tacgatcgga ctgtgctaca gcaaaatgga aaaacattga ctgattatga
    49801 agtaacagat atcgggaccc caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct
    49861 tcattatcaa acgccaatct atttctattt cattctttct cttactttga ttggctggtt
    49921 taccttgcta tgtttattta tttatcatag atacaagctt ttacgtgctt aggcaaaagg
    49981 gataaataca tccgatcaaa aaactctcca aacggtataa tcgtttggag agttttattt
    50041 taatcagttt ttttatgtta tatgttatat tttttattcg ttcacatcta attcgatttc
    50101 caccccgtag tgatcactga tgattggact atcgatccca tcaaaaatcc gttgatattt
    50161 tttaacctgc attccttcag ggacgaaaat aaaatctata cgcaattttt ctgtattttg
    50221 ttcccaacca tctattttct tttccacagt agctgctttt cctttttcat tcgcgacaac
    50281 atacgaatct tgaagaagaa ggcaactttc tcttacagta taataacctt gagagtctac
    50341 tggattattg aaatcaccta ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc
    50401 caagtttttc cactcaaaat aaaagccact ttttttatct gtccaccaag aaaagtggca
    50461 gctcgagaca aaaatgtcct gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt
    50521 ctgataatct tctttctttt gaaattggga gacggtaagg acctgacttt ccaatgtttt
    50581 tttcgtaagc agtgcatttc cttcttcata tttatcataa ccgatgtggc tcatttccca
    50641 actccaaaaa tactcttttc cgtgttcctt taaaagctga actaaacggt aggcaaaatt
    50701 atctttacgg ataggtactt gattctttac cggacagaac ttcattagct tcgcaggttc
    50761 tacttcttca gattccagta attggttgat ttcttgtaaa gcaatcactt cataatcttc
    50821 taacaggatc ttatctgcta attcttgcaa ttttttctct ggttctggct caagccagct
    50881 atgcgtgttg agtgtcatga atttcattgg ttttctcctt gtcttcattt cgaaacaaaa
    50941 gggttgtgac aaaatttttg tcacaacctc tccagcaaat agtgttctcc tgctaactcc
    51001 tcagtaataa gctcaatgga ccaggagcta ttttttgttt ggctgttttt atcacttggt
    51061 gcagggcagc tttttcacga cctctccact aaacagtatt caaactgctg cttctcggta
    51121 ataagcccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt cttatgactt
    51181 gatgcaggac agctttttca caacctctcc ttacatttta atggagccga tcactttacc
    51241 agctgtcact gtacctaatt gcttgatcac tacttcttct actttatcgt ttgtaaatac
    51301 gatcatgatc gttggatctt tttcttcgtt ttggatcaca tcaagatcca tctcagctaa
    51361 tttcgttcct gttcctactt cttggccttc tttgacagca atctcaaaag ctggctgatt
    51421 catttggact gtatcgattc ccatatggat caaaacttcg ataccatctt tcgtttccag
    51481 cccaattgca tgtttgctag ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac
    51541 ttctcttgat gttggaataa cagcaaagcc gtcacccatc attttttgtg agaataccgg
    51601 atcttttact tcagataaag gaatcacttc tccagaagct gcggaaacta gttcttgttc
    51661 tatacctaag tattgcttac tacctgcttg tccatttcct gtagaaacca tttctggctc
    51721 tggaatctct gctcctgatt gcagcagatc ttcgatatct gatttcaaga catctgcttt
    51781 tggtccataa actgcttgga ctccattgtc tttgacgatc aatcccatcg cccctgctcg
    51841 tttccaggct tcttcagtcc ctactttttc tcgatcattt acacttacgc gtaaccgagt
    51901 catacaagca tcgacatctt tgatattttc ttttcctccc agcaaatgaa tgatctggac
    51961 cacttgctga tctgctgttc ctgttccgct gttagataca acggtttctt cactgtcatt
    52021 gtcgtaattt ccgttacgtc ccggtgtagc aaagttgaat tttttgatca agaaatttgc
    52081 taagaaatac gtaacaacac caaaagcgat cacacacaac acgaagttga taagatctcc
    52141 acctagaccc gctttgatag ctaacggtgt ccgagtcagc aattcgatat tcccaaatga
    52201 atggacccgc aatggcaaga tatctgccat cgcaaatgca gccccttgga tcactgaata
    52261 aatgacatat aatggcacag cagcaaacat aaacatgaac tccaatggtt ctgttacacc
    52321 tgttagaaag acagccaacg ctgctgaaaa atacattgat ttatatcttg ctttcttatc
    52381 aggatcgaca ttacgataca tcgcaaaagc cattcccatc aagatacctg aagaaccaat
    52441 catttgtcct actttgaaac gagccggcgt ccagttatgc aacacaaact catattgcga
    52501 catatcccct gcacctttaa gattcactaa atcagtggcc caagccaacc ataacggatc
    52561 ttgcccaaat acttgtgtcc ccgcctgtgc gcctgataaa atctcatacg atccgccaag
    52621 ctgtgtatag ttgataggaa tcgtcagcat atgatgcaat ccaaatggca atagtaaacg
    52681 ttctaatgtt ccaaataaaa acggtgctag aatcggtgcg ctttcttgtg attctgcaat
    52741 ccataaacca aaattattga ttccagcctg tacgtttggc cacaccacag caagaatcaa
    52801 tgccacgatt gttgaccata agataacaac aaacggaaca aagcgctttc cattgaaaaa
    52861 tgacaaagca tccggcaatt ttctataatt atagtactta ttataagcca ttgccccgac
    52921 aaatccagcg atgatcccta cgaatactcc catattcaaa gctggtgctt ccaatacact
    52981 tgtaaaaaat cctttaacca tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc
    53041 attcgcaagc atgtcacttg tgacaccaaa gatagcccca gtgatccgat tgattaaaat
    53101 aaatgagatc ccagctgcaa atgctccgcc tgctctttct tttgcccaac ttcctcctat
    53161 tgctaaagca aataaaatat gaagattacc aataattgcc caaccaatat tttcaattac
    53221 tccgcccgtt gtgacaagcg gcgtccaatc aggattgatc aagggaattg atttccctat
    53281 actgatcatc aacccagctg ccggcataac tgctaccaca accattaaag cctttccaaa
    53341 tttttgccaa aactcaaaac taaatatctt tttcattccg tttagctcct ttacgcaatc
    53401 ggtttcgtta aacgtattat agcgtgcgtt ttcttttttg taaatgcttt attttttatt
    53461 tttctgtcta taccattcta taaaacctat aatacgtgtg ttcccttcta taattttctc
    53521 ctaaaaattt atttttttaa gcttttttct atgaaatatt ttcttttgtc tattgacttg
    53581 tgcaatcggt tgcgtttatt atgaacgtat aaaacagctc attaggagga gaaaaatgaa
    53641 acagatcaaa cgactatttg ctattgatcc atggaaaatc agcacgacac aattagataa
    53701 aaaaaatctt cgactgcaag aatctttaac aagtattgga aacggttata tggggatgcg
    53761 cgggaacttt gaagaaactt actcagggga ccaccatcaa ggtacttatt tggcgggtgt
    53821 ctggtatccc gataagacac gtgtcggctg gtggaaaaat ggttatccag aatactttgg
    53881 aaaagtgatc aacgccttga actttatcgc gatagatatt tcgatcaacg atcagctgat
    53941 cgacttggct tctttggaac cagaagactt ttactgggaa ttagatatga aaaatggtgt
    54001 attatctcgt acattcacaa tcactacttc tactaataaa gtacgcattt ccttcgaacg
    54061 ttttctgagc attgtcaaaa aagaatcagc ttatatctat ttaactatag aaatgcttga
    54121 aggtagcgga acagtcaaac tcgtatcaaa attggatgga aatgttcaaa acgaagacag
    54181 caactatgat gaacattttt ggaatgagat tgatcgtgga actatagaag aaatcggcta
    54241 tttgacgact aaaactattc caaacaactt tgggattgaa caatttactg ttactgcagc
    54301 aatgcgccat ttcgttaatg gaaaatcagt catccctgac tatcacgaag atactttagc
    54361 attacatggt tgcttgtctg cagaattatc tcaaggtgag acatggtcag tggaaaaaga
    54421 aattatcgtt cttactagta gagatattcc agaagcgcag caagcagaaa aagctgtctc
    54481 agaattaacg aaccagacaa acaattatat ccaagcaaaa gaagagcaaa cagcggcttg
    54541 ggcagaacga tggaagcttg ccgatgtggt catcgaagga gacgaagaag cacaacaagg
    54601 tattcgattc aatttgttcc aactattctc tacttactac ggcgaagatg aacgattgaa
    54661 tatcggcccc aaaggattca caggagaaaa atacggcgga gccacttact gggatacaga
    54721 agcgtatgct gtccctcttt atcttgcttt agccaaacct gaagtaacta agaatctgct
    54781 gaaatatcgg cataatcaac tgccgcaagc aatccacaac gctcagcaac aaggattaaa
    54841 aggcgcgctt tatcctatgg tcaccttcac tggtgtcgaa tgccataacg agtgggaaat
    54901 cacattcgag gagattcacc gaaatggcgc gattgcctat gcaatctaca attatacgaa
    54961 ttacaccggc gatacggaat atttgaaaaa tgaaggactg gaagttttag cagaaatcgc
    55021 acgcttctgg gcagaccgtg tccatttttc aaaacgagca aataaataca tgatccacgg
    55081 tgtgaccggc ccaaacgaat atgagaacaa tatcaataac aattggtata caaatacgat
    55141 cgcagcttgg gtcttgcgtt acacgcgaga aaattattta acttatcaaa aagaagctgc
    55201 tatcactatc agcgaagaag aactagccaa atggaaagat atcgcagaga acatgtactt
    55261 cccagttgac gaagaattag gaatctttgt ccagcatgac acattcttag ataaagatct
    55321 catgcctgta tccgatattc ccacaagcga tcttcctctg aatcagcatt ggtcttggga
    55381 caaaatcctt agatcttgct tcatcaagca ggcagatgta ctacagggga tttatttctt
    55441 caatgatact tttacaaaag aagaaaagca aaaaaacttt gatttctatg aaccaatgac
    55501 tgtccatgag tcttcccttt cgccaagtat ccattcgatt ttagcagcag aattaggaat
    55561 ggaaagcaaa gctgtagaaa tgtaccagcg aacagctcgc ttggatttgg ataattacaa
    55621 taacgacaca gaagacggct tgcacatcac ttccatgact ggtagctggt tgtcgattgt
    55681 ccaaggattt gctcaaatga aaactgccca tgatcagttg agattcgcac catttctgcc
    55741 aaaaacttgg aaagcttatt ctttccatat caattatcgc ggacgtttat tgttcatctc
    55801 agtcacagat gaacaagtta ctttcgagtt attagaaggt tctcctctag aaatttacgt
    55861 ctatgaacag tcttattctt tagcggatcg tctagaaata acgctaagaa aggaggaagc
    55921 aaatgtttaa aggggtattg tttgatttag acggtgtcat taccgatact gctgaattcc
    55981 attatcgtgc ttggcgaaag cttggtcagg aaatcggcat tacgatcgac cacacattca
    56041 acgaacagct aaaaggcgtc agccgagaag actcactctc tttgctttta gcttatggcg
    56101 gaaaagaaca ctctttttca aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt
    56161 attatttaga gatgatccag acgatcgaac caaaagatgt cttccccggg gtcgtgcctt
    56221 tactggattc tttaaaagaa gaaaaaatca agattgctct tgcttctgca agtaaaaacg
    56281 ggccattcct acttgaaaaa atgggtctta caccttactt tgatgctatt gccgacccgg
    56341 cagaagcaac aaacggaaaa cctgcgccag atatcttcct ccttgcagca aaagctgtcg
    56401 gactaaaagc agaagaatgt atcgggatcg aagacgcgca agccggtatc caagccatcc
    56461 tctcaagtgg cgctcaacca attggcgtgg gacgtgctga agaccttgga gaggagatcc
    56521 cgcttgtccc tgatacgact tttcttacga tcgattactt gaaagaaaag tggcatgacc
    56581 atggataatc cagagatctc cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca
    56641 ttgaagaacg actacttaga agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc
    56701 ttcgcaccag acaaagaagg aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa
    56761 gatgttctaa cagacaaaag cttttttgga gcaactgtcg gacctgtagc cggaaggatc
    56821 cgggatgctt cttggggtga gcatagattg gagaaaaact gcggatcaca ccatatccac
    56881 ggtggcacga aaggctggtc tttccaattt tgggaagtag aagttttcaa aactccatca
    56941 tcaataggtg tggtctttta cttaaaagat gaattttcta cctatccagg accaatcact
    57001 gctacgatca cttaccgttt aacaaaaaac gaattagaga tgacaacctc tgctagtagc
    57061 cttgttgaaa ccatctgcaa tccgaccaac catgcctact tcaacctttc aggaaatgga
    57121 acaagagaca tctatgaaca tcagttaacg gtctttttag atggtgtgct tgaattagat
    57181 caagaaaaac tgcctactgg aaattggaca aaaaaagaaa aactgccaat cgactttaga
    57241 aaatccccaa cactgcaaga gatccttgca tgttatccag atggactaga tgatgtattt
    57301 cttttgcatc atcctaggct ttccaaaact tccttacaat tattcgagaa gcattctggt
    57361 cgccaaatga ctatagcaac ttctaataaa agtatggtat tattttctac gactggattc
    57421 gaagctgatt tctctgtcaa tggaaaacag atgcattcaa actacggatt agcaatcgaa
    57481 ccgcaagaaa tccctgatat cgttcatttc cccaaatttg gatcgatcaa cctccatcct
    57541 ggtcaagaac ggatcagtca gacgatttat cggttctctg cacaataaca agagaaaggt
    57601 gtttttatca tggctattac cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg
    57661 accgtctctc gtgtcatcaa cgatcatcct agtatttccg agacaacaaa gaagaaagtc
    57721 cgaaaaatta tggatgaact aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa
    57781 cgcatttcca gtgccatcgg cgtaatctta ccccctcttg attcaaaaga gcggatcggt
    57841 aacccatttt atcttgagat catggaagca atcaacgaag aagcgcggaa ttatgatatg
    57901 actacagcaa tcgctactgc taaaaatttt gaattgctgt tagagaacgt ccagcgtatg
    57961 catttacaaa aacaagtaga tggttttatt ctagtgtatt cagatagtca agatactgtc
    58021 attgattatt tataccaaaa ccacatccct ttcactttga tcggacagcc ttatcatcat
    58081 gagaacgaaa tcgtttatgt agataatgat aaccagcttc taggtaaaca agcaacagaa
    58141 tttttgatcc agaatggaca taagaatatc ctgtttgtga caaacacgac tcatgaaagt
    58201 ttgtatttcg aacgatattt cggttatcaa aaagcaatga tgatggctgg tcttcctgcg
    58261 ttatccagtg tagtactaga gcatccggag gattatctgt cctttgagaa aacgttgaaa
    58321 gaaaacgaag ccacagcatt ggtggtcatt gacgatatct tcgcccttcg cacgatgcag
    58381 ttggcgcaaa tgtatggcta tgtcattcca gagacactct ccgtcattag cttcaacaac
    58441 tcgatcttct caacattaac acacccttac cttacaagta tcgatatcga tatctccgaa
    58501 ttaggacgtg tcgctatgca aaagctgcgc gaattgatcc atgaagaatt gagcagtggc
    58561 gtacgtttgg tcatccctca tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac
    58621 ccatactaaa tagctgactc actaaatacg ctgaccattt atcctatggt cagcttttta
    58681 tgtaaaagaa gatactactt ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa
    58741 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
    58801 tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt gagtttcttc
    58861 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt caatttttta
    58921 cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca agaatcgaga
    58981 aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata atgaatgtgc
    59041 cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct agtgttcgtg
    59101 tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa
    59161 gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag ctcaaggcag
    59221 taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga acgatggtat
    59281 caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa atcaaactag
    59341 cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc tttggatcag
    59401 gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt
    59461 tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat gtagggatag
    59521 ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat gtccaaggag
    59581 agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct agatcggctg
    59641 aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat gctgggaaat
    59701 ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata cccgctgcca
    59761 agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg gaaacgactg
    59821 cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc
    59881 caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt gcacaaacaa
    59941 gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa accataatcg
    60001 tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa agaaaaatca
    60061 ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc tcatcccatg
    60121 tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg atcgggatat
    60181 gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac acgatcaata
    60241 cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta tttcaaatga
    60301 aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa tcagcgacaa
    60361 aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct acctcaatcc
    60421 cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg accactgtga
    60481 tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca aatacgacaa
    60541 cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt ccgtttttac
    60601 caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc aatgaaacag
    60661 caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt gtcaagaata
    60721 cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata acgattgatt
    60781 gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat ccgaattttg
    60841 gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca gtaacccaag
    60901 ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt ccgatcactt
    60961 ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt gctaattcca
    61021 tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc aataaaacta
    61081 atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct ttcaaacgag
    61141 gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca gctaagtatt
    61201 tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag tcacctagat
    61261 agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg aacatgatga
    61321 ctactgggat atatacccct agccatacac cgatgttccc gccgactttt gccatatcga
    61381 atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct aaaacgaacc
    61441 agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt cctaaagtag
    61501 aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca acgacaaatc
    61561 cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct tgaggaagca
    61621 tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa actgcaaaaa
    61681 ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc cgaaccgttg
    61741 cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt acttcagatt
    61801 tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct ttgaagtagt
    61861 ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag tttattgctt
    61921 gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag gtctcccctt
    61981 gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt tctaacatga
    62041 ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt tttccaattt
    62101 ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt ctttgttcat
    62161 gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt tccattcttc
    62221 atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt ctgggatagc
    62281 aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca cgtatgaaga
    62341 ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt tgcctttttc
    62401 tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa cttcgatttc
    62461 tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat gtgcaccttc
    62521 gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga catggtgtgc
    62581 tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat aagccccaag
    62641 taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag agataacgtc
    62701 tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat agcccatttt
    62761 atgtgggtca atcgttacag attctgcttc ttcgattgct ttatatgcac tgtgtacttc
    62821 ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa catcttttaa
    62881 atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc
    62941 ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata cgcgacgcaa
    63001 tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta caacaccgac
    63061 tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt caagttcgtt
    63121 gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta aaccgatacc
    63181 gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca accattttcc
    63241 taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt cgattttttc
    63301 tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta attcccaatc
    63361 gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca atgatttgat
    63421 gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc
    63481 ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt cttccatttg
    63541 gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca tagcaaaatt
    63601 gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt aacgtccggc
    63661 attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata gaacatcctt
    63721 tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg taataatagg
    63781 catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca attcattcaa
    63841 caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg catttaggtt
    63901 tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata tttaaatttt
    63961 gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg cctgattttt
    64021 aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa taattaaaaa
    64081 aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg acaaccagcg
    64141 ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc tttttcaagg
    64201 taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt cttacctttt
    64261 cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa atttaaatcg
    64321 gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg tgcttgacgt
    64381 ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg tgtgcttgaa
    64441 atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt tagttcttta
    64501 atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta
    64561 tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct
    64621 gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt aaagaatttc
    64681 aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa ctcaaatggt
    64741 gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa tttcgttccg
    64801 tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat
    64861 tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc accgatttgc
    64921 aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag gatttggtaa
    64981 gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc ttttgctaac
    65041 atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa agagattttt
    65101 gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa caattttctc
    65161 atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg caacacacgt
    65221 tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat cagaatatat
    65281 ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa atgtccgata
    65341 tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt ttcttcaacg
    65401 agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca agttaattca
    65461 tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag gaacctgcta
    65521 gcatatgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt cccttggcat
    65581 ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga tatccaacat
    65641 acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa
    65701 ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac attggcttta
    65761 acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat catagagcgc
    65821 aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt ttttcttttt
    65881 tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa ataacttttt
    65941 ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat tctttcttcg
    66001 ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa
    66061 aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt ttaattctaa
    66121 gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa
    66181 taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact caagcaaaaa
    66241 atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa gatagtaaga
    66301 acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa gaacaactga
    66361 tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg atcgtggacc
    66421 cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg gtgactgttg
    66481 aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta gttaatttga
    66541 atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt gcgctacctg
    66601 aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa tatatggata
    66661 attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa aatgtctata
    66721 aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc gatgcctttg
    66781 tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt
    66841 acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat gaaaaaacaa
    66901 aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac ggctggagct
    66961 ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa gatgtaaaaa
    67021 aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca aatattctga
    67081 tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa aatcaaaaat
    67141 ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct tctacaccag
    67201 cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc gatggcattc
    67261 gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc aatgtccatg
    67321 atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa
    67381 agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc ttcttcggtg
    67441 cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt tttgcgtata
    67501 tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag aactcatccg
    67561 aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca agcatgatac
    67621 tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt tctttttttg
    67681 tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga taacggattt
    67741 tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca atcaacgctt
    67801 tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc agcccttcct
    67861 tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca gctaccttat
    67921 ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa
    67981 tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga gcctcccgta
    68041 attttaaact tttataatat atatagttca aatttattct gaggtttatt ataactatct
    68101 attgagaaag tatcaagcta gcaattcctt gtttatgaat tcattgacag aaatttataa
    68161 aaagaatatt atgtactaaa ataataaaaa aagttttata aaaaggtata aaaccttttt
    68221 attaaactat ttaatcttgt ttagtatata tttttagaaa aaggagtggg gtttttatgg
    68281 cagaaatcgt caaagtcaca catttgaaaa agagctttgg tcgtttcgaa gctttaaaag
    68341 atgtctcttt caccgtacat tcaggtgaag tcgttggatt tatcggacca aatggttctg
    68401 gaaaatccac gacgattcgg acacttttag ggattataaa aaaagacggc ggaacggcaa
    68461 cgatttttgg gcaagatgta tgggaagata gtttatctat ccatgaacgt ctttcttatg
    68521 ttccaggcga tatctctttg tggggaaatt tgactggagg agcgatcatc gatctcttca
    68581 tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga actgattcaa cgtttctctc
    68641 ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga
    68701 ttgctgcttt ggctgttgat tcggatctct atttatttga tgagcctact tctggtttag
    68761 atcctttgat ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa cagggcaaat
    68821 ccatcctact ttcttctcat attctcagtg aagtcgagcg actagcggat aaagtcgtga
    68881 tcatacgcga aggaacactc gatgaagaag gaacactcga tgaattgcgt catttgactc
    68941 gttcgaccat cactatagaa acagaagcag agaatagtca cttagcacaa ttgaacggtg
    69001 tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac gaagctttcg
    69061 atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa atttgaatct attccaccga
    69121 ctcttgagga tttattcatg agacattatg aaggatgagg aggaagcgaa atgactgata
    69181 aattttcacg ctggtccacc cttttttctc aaaaccttag ccgtgactgg aaaaagatcc
    69241 tgatatggct agtcggtgtc actgcttttt ctggtggttt tgtccctgcg tttgaagaat
    69301 tgacttccgg acaaggatta gctggattat acgaaacctt gcaaaacccg gcaatgattg
    69361 caatggtcgg accaactcca gcttcgaatg ctgctgagta tactttagga gcgatgtatg
    69421 caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg
    69481 tgagccaaac gagaaaaaaa gaagaacttg gtctgattga acttattcgt tcctttcgag
    69541 tcggacgtca agcaaactct ttagctgtca tcgctgaaag tattctgatc aatttgcttt
    69601 tagcaatatc gatcagctgt acgatgattt ttttccaagc ggattcgatc actgcagaag
    69661 gtgcttggct ttttgggcta tctgtaggta gtatggggat tttggggagt gccgttgcac
    69721 tgtttgctgc acaactgatg ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt
    69781 taggtggact ttaccttcta cgagcaataa cagatgtagc tgattcctcc ctttcgatgt
    69841 ggaatccttt aggctggtct tatttgacct atccatttac aactaacaac tggattcctt
    69901 tgctttattt gtttttgttc agtctaatct tgataatcag ttcatttgtt ttagaggaac
    69961 atcgagattt gaacaccggc tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt
    70021 ctcttttatc cttgcgtggt ttattattct atctaaataa agggatgaca atcagttggc
    70081 ttatcacttt tggtttgatg ggggcggcat acggctctat ctacgggaat atgcaacagt
    70141 ttttagaaag taacgactta atgaaacaga tgtttatcca atctggcgtc tcaattgaaa
    70201 aaaactttac aagcacgatc atggtagtct tgatttgtct agctgctatt ttaccaattg
    70261 ctagcatcaa tcgtttattt acagaagaaa cccacggacg tatgaatcag ctgttcgcaa
    70321 caaaaatttc taggaaccaa ctttattgga ccaatatgtt actagctatt ctatttgcta
    70381 tattggcaat cctgattagt tctggtgcac tagggctttc tgctttggct tcgatgaagg
    70441 atgcgaaaga catggatcta ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg
    70501 ttttagtgtt tatcggaatt agtggctttc tattcggatg gcttcctcga atcggtaaac
    70561 ttgcttatat ctacttagct tactcctttg cactcaatta ctttggcgga attttggatt
    70621 taccagaatg gttttccaaa acagctgtcc ctagctgggt tccacggatg ccaaacgatt
    70681 cttttgatct ttccgtgttc cttacgttat catgtatcag ccttgcactg ttcgtccttg
    70741 gttctattgg ttataggcga agagacttac tagaaggata ataaaaaagc atgtacaatt
    70801 tcatgctagc ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt tttttattta
    70861 caagtcttct aactgctctt tatcaatgat ccagacttct cgattctttc tttttacata
    70921 accattctcc tctaataatt taaattttct agataaagtt tcgggggtcg taccgataaa
    70981 tgcagcaagt tctttcattt tcattggcag gaaaaagtga tccttccctg tcgctttagc
    71041 taagtcaagc agatagacag cgactcgttc ttctactttt tccatcatca aaaaagttgt
    71101 ctgctgctgt acttttgtta atttctcaac gttgattgct aaaagtttca gactaagggc
    71161 gggataatgc agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt
    71221 ttctagtgct tcgccaaata aagactcatt tggaacgcct agcaactggc tctctccctc
    71281 atatccgccc ggctctatta ctcgtaaaag ctgttccttt ccgctagcag ataattgata
    71341 cactttcata ctgccggagg ccacgatcac taattgaggt tctccattcg gcgcaagaat
    71401 ctgttctcct ttttccacat agtgatgttt gacaagtgcg tgtacttttt cttgatcttg
    71461 ctgtactaag tgattgaaca aaggaacaaa tgaaacacat aaatgatcat gactcataat
    71521 ctctcctcct tctaaacagc tatttctaat aaagaaacat aacttccctt attccaactg
    71581 ataattttct tttatcatag cataaacgtc aagcgaacca gataccagca aatagagtgg
    71641 atttcttttt gtcccttgct ttattttcct tcttttgaga ccttaagtaa ttgattccta
    71701 tcaatttttt aaatatttac tttttgaata atcaaaaaca aaccttcact cgcaagccga
    71761 gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt tttccgcacc
    71821 tagcttttga tatagtggtg ctagttcttg taaggtttca gcggttattt gctcaacgtg
    71881 ttcgatttct tgcggataga agaatagttt acccatttga aaggttcggt ctctcatcgc
    71941 ttttttcaac tcttcttgtt tctttgtatc ggacaaatag tcagacaaag ctaagtggtc
    72001 gtcaagttca tgttctggtt gcggccatat gtaataatcc ttgatatcga ctgttttgct
    72061 ccactctttc agtatcgcaa gcaactggtt atacgctgca atgctaccat atcgttcttc
    72121 gcttttataa tgttggtaca tcaaatagat ttggtattgt ttttgattca acaaaactgc
    72181 tagacaggca ttctcgtctt gacgagattc gcttcggtac gcgctccaaa aatgactgcg
    72241 taaattccag ccattcgtcc agctttcgat tttaggtttg ctcatgccgt acatatcagg
    72301 caaaagtgca gctgtctgga gctgaatctc tttccatttt tgccagtgtt ctttatattc
    72361 tttcttgatt tgttcaatct tatctgttgg atggctctct ttgagctgtt tgaatgcgta
    72421 gattggtcga tcaaacaact gataatctga ttttccaagt tccattgttt cgcctctttc
    72481 cgttatcttt tgtattctac ctgaattttg cgatatggac aaatagaggt tgtgaaaaag
    72541 ctgttctgca tcaagtcata agaacagcca aacaaaaaac tgcgatgagc atcaccgcag
    72601 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
    72661 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
    72721 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
    72781 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
    72841 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
    72901 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
    72961 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
    73021 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
    73081 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
    73141 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
    73201 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
    73261 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
    73321 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
    73381 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
    73441 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
    73501 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
    73561 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
    73621 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
    73681 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
    73741 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
    73801 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
    73861 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
    73921 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
    73981 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
    74041 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
    74101 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
    74161 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
    74221 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
    74281 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
    74341 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
    74401 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
    74461 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
    74521 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
    74581 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
    74641 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
    74701 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
    74761 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
    74821 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
    74881 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
    74941 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
    75001 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
    75061 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
    75121 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
    75181 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
    75241 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
    75301 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
    75361 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
    75421 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
    75481 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
    75541 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
    75601 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
    75661 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
    75721 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
    75781 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
    75841 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
    75901 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
    75961 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
    76021 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
    76081 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
    76141 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
    76201 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
    76261 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
    76321 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
    76381 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
    76441 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
    76501 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
    76561 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
    76621 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
    76681 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
    76741 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
    76801 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
    76861 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
    76921 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
    76981 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
    77041 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
    77101 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
    77161 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
    77221 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
    77281 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
    77341 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
    77401 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
    77461 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
    77521 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
    77581 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
    77641 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
    77701 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
    77761 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
    77821 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
    77881 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
    77941 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
    78001 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
    78061 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
    78121 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
    78181 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
    78241 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
    78301 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
    78361 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
    78421 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
    78481 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
    78541 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
    78601 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
    78661 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
    78721 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
    78781 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
    78841 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
    78901 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
    78961 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
    79021 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
    79081 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
    79141 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
    79201 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
    79261 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
    79321 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
    79381 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
    79441 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
    79501 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
    79561 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
    79621 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
    79681 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
    79741 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
    79801 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
    79861 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
    79921 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
    79981 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
    80041 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
    80101 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
    80161 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
    80221 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
    80281 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
    80341 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
    80401 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
    80461 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
    80521 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
    80581 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
    80641 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
    80701 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
    80761 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
    80821 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
    80881 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
    80941 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
    81001 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
    81061 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
    81121 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
    81181 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
    81241 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
    81301 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
    81361 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
    81421 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
    81481 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
    81541 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
    81601 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
    81661 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
    81721 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
    81781 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
    81841 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
    81901 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
    81961 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
    82021 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
    82081 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
    82141 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
    82201 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
    82261 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
    82321 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
    82381 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
    82441 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
    82501 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
    82561 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    82621 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    82681 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    82741 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    82801 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    82861 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    82921 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    82981 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    83041 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    83101 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    83161 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    83221 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    83281 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    83341 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    83401 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    83461 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    83521 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    83581 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    83641 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    83701 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    83761 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    83821 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    83881 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    83941 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    84001 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    84061 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    84121 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    84181 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    84241 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    84301 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    84361 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    84421 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    84481 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    84541 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    84601 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    84661 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    84721 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    84781 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    84841 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    84901 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    84961 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    85021 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    85081 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    85141 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    85201 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    85261 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    85321 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    85381 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    85441 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    85501 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    85561 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    85621 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    85681 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    85741 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    85801 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    85861 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    85921 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    85981 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    86041 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    86101 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    86161 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    86221 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    86281 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    86341 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    86401 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    86461 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    86521 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    86581 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    86641 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    86701 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    86761 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    86821 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    86881 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    86941 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    87001 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    87061 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    87121 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    87181 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    87241 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    87301 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    87361 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    87421 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    87481 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    87541 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    87601 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    87661 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    87721 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    87781 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    87841 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    87901 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    87961 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    88021 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    88081 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    88141 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    88201 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    88261 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    88321 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    88381 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    88441 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    88501 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    88561 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    88621 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    88681 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    88741 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    88801 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    88861 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    88921 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    88981 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    89041 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    89101 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    89161 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    89221 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    89281 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    89341 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    89401 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    89461 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    89521 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    89581 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    89641 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    89701 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    89761 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    89821 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    89881 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    89941 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    90001 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    90061 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    90121 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    90181 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    90241 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    90301 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    90361 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    90421 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    90481 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    90541 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    90601 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    90661 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    90721 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    90781 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    90841 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    90901 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    90961 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    91021 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    91081 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    91141 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    91201 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    91261 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    91321 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    91381 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    91441 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    91501 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    91561 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    91621 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    91681 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    91741 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    91801 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    91861 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    91921 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    91981 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    92041 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    92101 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    92161 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    92221 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    92281 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    92341 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    92401 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    92461 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    92521 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    92581 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    92641 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    92701 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    92761 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    92821 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    92881 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    92941 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    93001 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    93061 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    93121 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    93181 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    93241 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    93301 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    93361 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    93421 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    93481 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    93541 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    93601 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    93661 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    93721 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    93781 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    93841 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    93901 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    93961 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    94021 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    94081 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    94141 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    94201 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    94261 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    94321 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    94381 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    94441 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    94501 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    94561 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    94621 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    94681 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    94741 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    94801 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    94861 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    94921 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    94981 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    95041 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    95101 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    95161 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    95221 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    95281 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    95341 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    95401 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    95461 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    95521 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    95581 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    95641 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    95701 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    95761 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    95821 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    95881 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    95941 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    96001 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    96061 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    96121 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    96181 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    96241 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    96301 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    96361 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    96421 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    96481 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    96541 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    96601 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    96661 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    96721 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    96781 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    96841 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    96901 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    96961 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    97021 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    97081 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    97141 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    97201 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    97261 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    97321 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    97381 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    97441 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    97501 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    97561 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    97621 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    97681 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    97741 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    97801 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    97861 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    97921 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    97981 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    98041 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    98101 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    98161 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    98221 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    98281 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    98341 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    98401 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    98461 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    98521 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    98581 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    98641 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    98701 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    98761 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    98821 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    98881 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    98941 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    99001 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    99061 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    99121 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    99181 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    99241 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    99301 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    99361 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    99421 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    99481 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    99541 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    99601 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    99661 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    99721 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    99781 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    99841 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    99901 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    99961 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
   100021 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
   100081 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
   100141 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
   100201 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
   100261 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
   100321 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
   100381 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
   100441 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
   100501 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
   100561 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
   100621 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
   100681 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
   100741 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
   100801 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
   100861 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
   100921 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
   100981 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
   101041 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
   101101 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
   101161 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
   101221 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
   101281 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
   101341 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
   101401 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
   101461 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
   101521 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
   101581 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
   101641 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
   101701 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
   101761 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
   101821 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
   101881 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
   101941 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
   102001 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
   102061 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
   102121 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
   102181 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
   102241 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
   102301 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
   102361 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
   102421 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
   102481 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
   102541 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
   102601 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
   102661 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
   102721 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
   102781 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
   102841 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
   102901 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
   102961 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
   103021 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
   103081 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
   103141 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
   103201 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
   103261 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
   103321 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
   103381 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
   103441 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
   103501 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
   103561 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
   103621 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
   103681 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
   103741 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
   103801 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
   103861 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
   103921 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
   103981 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
   104041 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
   104101 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
   104161 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
   104221 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
   104281 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
   104341 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
   104401 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
   104461 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
   104521 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
   104581 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
   104641 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
   104701 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
   104761 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
   104821 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
   104881 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
   104941 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
   105001 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
   105061 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
   105121 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
   105181 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
   105241 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
   105301 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
   105361 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
   105421 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
   105481 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
   105541 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
   105601 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
   105661 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
   105721 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
   105781 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
   105841 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
   105901 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
   105961 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
   106021 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
   106081 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
   106141 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
   106201 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
   106261 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
   106321 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
   106381 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
   106441 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
   106501 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
   106561 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
   106621 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
   106681 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
   106741 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
   106801 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
   106861 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
   106921 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
   106981 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
   107041 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
   107101 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
   107161 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
   107221 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
   107281 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
   107341 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
   107401 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
   107461 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
   107521 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
   107581 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
   107641 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
   107701 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
   107761 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
   107821 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
   107881 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
   107941 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
   108001 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
   108061 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
   108121 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
   108181 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
   108241 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
   108301 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
   108361 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
   108421 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
   108481 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
   108541 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
   108601 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
   108661 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
   108721 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
   108781 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
   108841 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
   108901 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
   108961 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
   109021 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
   109081 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
   109141 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
   109201 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
   109261 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
   109321 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
   109381 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
   109441 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
   109501 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
   109561 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
   109621 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
   109681 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
   109741 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
   109801 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
   109861 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
   109921 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
   109981 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
   110041 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
   110101 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
   110161 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
   110221 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
   110281 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
   110341 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
   110401 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
   110461 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
   110521 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
   110581 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
   110641 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
   110701 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
   110761 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
   110821 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
   110881 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
   110941 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
   111001 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
   111061 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
   111121 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
   111181 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
   111241 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
   111301 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
   111361 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
   111421 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
   111481 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
   111541 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
   111601 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
   111661 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
   111721 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
   111781 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
   111841 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
   111901 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
   111961 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
   112021 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
   112081 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
   112141 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
   112201 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
   112261 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
   112321 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
   112381 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
   112441 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
   112501 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
   112561 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
   112621 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
   112681 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
   112741 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
   112801 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
   112861 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
   112921 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
   112981 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
   113041 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
   113101 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
   113161 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
   113221 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
   113281 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
   113341 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
   113401 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
   113461 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
   113521 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
   113581 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
   113641 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
   113701 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
   113761 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
   113821 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
   113881 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
   113941 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
   114001 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
   114061 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
   114121 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
   114181 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
   114241 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
   114301 ccatgttttg atgtgcaccc caaaagttag actagaaatc taattaaaag gggtgctttt
   114361 tttgcggaaa tatacatttg tttttaagaa aaaagtagtt tcagactatt taaataacga
   114421 aggcggctac aaatatcttg cacataaata tcaaataaat cgtacactgg ttagacattg
   114481 ggtaaggatt tataactatc atggttggga aggcttggtt ggaggtggca aaagctacac
   114541 tacaaaattt aaacttgatg ttatagaata tatggaaaca aatggtcttt ctatccaaga
   114601 aactgctaaa aaatttaata tcggttcaaa tagaactcta agtaaatgga tagagcaata
   114661 tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg ggcagaaaaa ttagtatgaa
   114721 ttccaagcta aacattccta aaaaacttaa agatgagtct cttgaagaag aagttattcg
   114781 tttaagagca gagaacgcat atttaaaaaa gttagaaacc ttgattcaag aacaagattt
   114841 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
   114901 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
   114961 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
   115021 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
   115081 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
   115141 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
   115201 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
   115261 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
   115321 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
   115381 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
   115441 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
   115501 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
   115561 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
   115621 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
   115681 cggaaacagt ccattaaata gttcaacttt tggggttcag ttcataaaga gggcttttat
   115741 atattaatca caaatcactt atcacaaatc acaagtgatt aatcacaaat cacttgtgat
   115801 ttgtgattct taatgataca atattactat acaaaaaaag aatggggcgt agttatggag
   115861 aaggaaaaac taaaaatact tgaagaatta agacgtattt taaacaataa aaatgaagca
   115921 attattatct tgaataatta ctttaaaggt ggtgttggaa agtccaaatt atcgactatg
   115981 tttgcttact tgacagacaa attgaattta aaagttttaa tgatcgataa ggacttacag
   116041 gcaacattga caaaagactt agcaaaaact tttgaggtag aattgccacg tgtcaatttt
   116101 tatgaaggct tgaaaaatgg aaacttggct tcttctattg ttcatttgac tgataattta
   116161 gacttgatcc ctggcacgtt tgatttgatg ttactgccaa aattaactcg ctcatggact
   116221 tttgaaaatg aaagtagatt gcttgctact cttttagcac ctttaaaaag tgactatgat
   116281 ctcattatta ttgatactgt accaacgcca agcgtttata caaataatgc aatcgtggcg
   116341 agtgattacg ttatgatccc tttacaagca gaagaagaaa gtacaaacaa cattcaaaac
   116401 tatatttcct atttgattga tttacaagaa caatttaacc ctggactaga tatgatcggt
   116461 tttgttcctt atttagttga tacggacagc gcaacgataa aatcaaacct ggaagaactg
   116521 tacaagcaac ataaagaaga taacttggtt ttccaaaata ttatcaagcg aagtaataaa
   116581 gtaagtacct ggtctaaaaa tggaattaca gaacacaaag gctatgacaa aaaagtttta
   116641 tccatgtatg agaacgtatt ttttgaaatg cttgagcgaa ttattcaatt agaaaacgaa
   116701 aaagaataga atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgattgt
   116761 tgatgataaa ataagaataa gaagaaatag aaagaagtga gtgattgtgg gaaatttagg
   116821 cgcacaaaaa gcaaaacgaa atgatacacc aatcagtgca aaaaaagata taatgggaga
   116881 taagacggtt cgtgttcgtg ctgacttaca ccatattata aaaattgaaa cagcaaaaaa
   116941 tggcggaaac gtaaaagaag ttatggatca agccttagaa gaatatatac ggaaatattt
   117001 acctgataaa ctttaaaaag gagtgaaaat gaaatggcag ttacgtatga aaaaacattt
   117061 gaaatagaga tcattaacga attatcggca agcgtttata atcgagtatt aaactatgtt
   117121 ttgaaccatg aactagatac taaaaatact cgtttactag aagtgaatct tttaaatcaa
   117181 ttagaagtgg cacaagaagt tgatttattt caacaaccat ttgaagaatt acaagctatt
   117241 catgagtatt ggcggtcaat gaatcaatat tcaaaacaaa ttttgacaaa agagaaagtg
   117301 gcttaacatg gcgaatatta ctgacttcac cgaaaagcaa tttgaagatc gtttagaaaa
   117361 gaatgttgaa cgactaacta aaaatagact agcggttgaa tcgccaaccg cttttttact
   117421 tggtgggcaa ccaggatcag gtaaaactag tttgcgatcg gcaatttccg aagagacaca
   117481 aggaaatgtt gttatcattg ataatgatac gttcaaacaa cagcacccta attttgatga
   117541 actagtgaaa ctttatgaaa aagacgtagt aaaatacgtt accccttatt ctaatcgcat
   117601 gacagaagcg atcataagcc gtttgagaga taaagggtat aatttagtga ttgaaggtac
   117661 aggacgaaca acagacgttc ctattcaaac cgcaacaatg cttcaagcca aagattatga
   117721 aacaaaaatg tatgtcatgg cagtacctaa aatcaactca tatttaggaa caattgaacg
   117781 gtatgaaact atgtatgcag atgatccaat gacagccagg gcaacaccaa aacaagcgca
   117841 tgatattgtt gtcaaaaact taccgaccaa tttagaaacc cttcataaaa cgggcttatt
   117901 tagcgatata aggctttaca atagagaagg agtgaaactc tattcaagct tagaaacgcc
   117961 ttccattagt ccgaaagaga ccttagaaag agaattaaat cgtaaagtat cagggaaaga
   118021 aattcaaccg actttggaga gaatagagca aaaaatggtt caaaatcaac accaagaaac
   118081 ccctgaattt aaagcaattc aacaaaaaat ggaaagcttg cagccaccta caccaccaat
   118141 acccaaaaca cctaaacttc cagggattta aaccttaaaa aagtaaaaga gtagttacca
   118201 aaaaacggta actactcttt ttttataaaa acatttcctc attttttaaa agctgatcga
   118261 gaaataaagc acacaactgt attctttctt ttttcagttg gtacattttg cagtctgtaa
   118321 cgtgccattt actgtttgtt accttaacat atccagtcga aaacacaagg gtatataaac
   118381 gagcaagctc gcccttgtgt tttctttgtg tctatgttgc gttctcgtca cagcaaacga
   118441 cacgaacaca ctggcgaaaa tgaaccaaca aaacgaaaaa agaaaggagc gtacactgta
   118501 atttatttct agcttttaaa acatgaggaa aacagcttca cttcctcaaa cgcctaacaa
   118561 aaatggttgt caaaaaacta aaaatcaaaa aaagaaaaga ggaaaagaaa atggaaaaaa
   118621 caaaaaaatt acaattagaa gattttacag aaaatgg
//
LOCUS       DAZKOM010000002        77658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000002 DAZKOM010000000
VERSION     DAZKOM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.002"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1649)
                     /locus_tag="ACG8NF_000105"
                     /pseudo
     CDS             complement(14..1649)
                     /locus_tag="ACG8NF_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1972..2451)
                     /gene="rlmH"
                     /locus_tag="ACG8NF_000106"
     CDS             complement(1972..2451)
                     /gene="rlmH"
                     /locus_tag="ACG8NF_000106"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0470626.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2879..4168
                     /locus_tag="ACG8NF_000107"
     CDS             2879..4168
                     /locus_tag="ACG8NF_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0470627.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4207..4812)
                     /gene="ytpR"
                     /locus_tag="ACG8NF_000108"
     CDS             complement(4207..4812)
                     /gene="ytpR"
                     /locus_tag="ACG8NF_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0470628.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5017..5481)
                     /locus_tag="ACG8NF_000109"
     CDS             complement(5017..5481)
                     /locus_tag="ACG8NF_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0470629.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5503..5823)
                     /locus_tag="ACG8NF_000110"
     CDS             complement(5503..5823)
                     /locus_tag="ACG8NF_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0470630.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5967..7046)
                     /gene="pepA"
                     /locus_tag="ACG8NF_000111"
     CDS             complement(5967..7046)
                     /gene="pepA"
                     /locus_tag="ACG8NF_000111"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0470631.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7246..7596
                     /locus_tag="ACG8NF_000112"
     CDS             7246..7596
                     /locus_tag="ACG8NF_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0470632.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7819..8766
                     /locus_tag="ACG8NF_000113"
     CDS             7819..8766
                     /locus_tag="ACG8NF_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0470633.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8823..10241)
                     /gene="pepV"
                     /locus_tag="ACG8NF_000114"
     CDS             complement(8823..10241)
                     /gene="pepV"
                     /locus_tag="ACG8NF_000114"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0470634.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10390..11235
                     /locus_tag="ACG8NF_000115"
     CDS             10390..11235
                     /locus_tag="ACG8NF_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0470635.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11239..11679
                     /locus_tag="ACG8NF_000116"
     CDS             11239..11679
                     /locus_tag="ACG8NF_000116"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0470636.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11729..11923
                     /locus_tag="ACG8NF_000117"
     CDS             11729..11923
                     /locus_tag="ACG8NF_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470637.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12059..12745
                     /locus_tag="ACG8NF_000118"
     CDS             12059..12745
                     /locus_tag="ACG8NF_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0470638.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13153..13842)
                     /locus_tag="ACG8NF_000119"
     CDS             complement(13153..13842)
                     /locus_tag="ACG8NF_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470639.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13817..15946)
                     /locus_tag="ACG8NF_000120"
     CDS             complement(13817..15946)
                     /locus_tag="ACG8NF_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0470640.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15994..17331)
                     /locus_tag="ACG8NF_000121"
     CDS             complement(15994..17331)
                     /locus_tag="ACG8NF_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0470641.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17583..17942)
                     /gene="rplT"
                     /locus_tag="ACG8NF_000122"
     CDS             complement(17583..17942)
                     /gene="rplT"
                     /locus_tag="ACG8NF_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0470642.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17995..18195)
                     /gene="rpmI"
                     /locus_tag="ACG8NF_000123"
     CDS             complement(17995..18195)
                     /gene="rpmI"
                     /locus_tag="ACG8NF_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0470643.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18267..18791)
                     /gene="infC"
                     /locus_tag="ACG8NF_000124"
     CDS             complement(18267..18791)
                     /gene="infC"
                     /locus_tag="ACG8NF_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0470644.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18835..18960)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19111..19743
                     /locus_tag="ACG8NF_000125"
     CDS             19111..19743
                     /locus_tag="ACG8NF_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0470645.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19745..20650)
                     /locus_tag="ACG8NF_000126"
     CDS             complement(19745..20650)
                     /locus_tag="ACG8NF_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0470646.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20735..21394)
                     /locus_tag="ACG8NF_000127"
     CDS             complement(20735..21394)
                     /locus_tag="ACG8NF_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0470647.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21710..22654
                     /gene="mvk"
                     /locus_tag="ACG8NF_000128"
     CDS             21710..22654
                     /gene="mvk"
                     /locus_tag="ACG8NF_000128"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0470648.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22647..23624
                     /gene="mvaD"
                     /locus_tag="ACG8NF_000129"
     CDS             22647..23624
                     /gene="mvaD"
                     /locus_tag="ACG8NF_000129"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0470649.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23637..24722
                     /locus_tag="ACG8NF_000130"
     CDS             23637..24722
                     /locus_tag="ACG8NF_000130"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0470650.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24719..25783
                     /gene="fni"
                     /locus_tag="ACG8NF_000131"
     CDS             24719..25783
                     /gene="fni"
                     /locus_tag="ACG8NF_000131"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0470651.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26032..26835)
                     /locus_tag="ACG8NF_000132"
     CDS             complement(26032..26835)
                     /locus_tag="ACG8NF_000132"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0470652.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26855..27697)
                     /gene="kduI"
                     /locus_tag="ACG8NF_000133"
     CDS             complement(26855..27697)
                     /gene="kduI"
                     /locus_tag="ACG8NF_000133"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0470653.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27713..28720)
                     /locus_tag="ACG8NF_000134"
     CDS             complement(27713..28720)
                     /locus_tag="ACG8NF_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0470654.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28727..29371)
                     /locus_tag="ACG8NF_000135"
     CDS             complement(28727..29371)
                     /locus_tag="ACG8NF_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0470655.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29471..30229)
                     /locus_tag="ACG8NF_000136"
     CDS             complement(29471..30229)
                     /locus_tag="ACG8NF_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0470656.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30356..31507)
                     /gene="fucO"
                     /locus_tag="ACG8NF_000137"
     CDS             complement(30356..31507)
                     /gene="fucO"
                     /locus_tag="ACG8NF_000137"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0470657.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31589..31900)
                     /gene="rhaM"
                     /locus_tag="ACG8NF_000138"
     CDS             complement(31589..31900)
                     /gene="rhaM"
                     /locus_tag="ACG8NF_000138"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0470658.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31902..32747)
                     /gene="rhaD"
                     /locus_tag="ACG8NF_000139"
     CDS             complement(31902..32747)
                     /gene="rhaD"
                     /locus_tag="ACG8NF_000139"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0470659.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32761..34017)
                     /gene="rhaA"
                     /locus_tag="ACG8NF_000140"
     CDS             complement(32761..34017)
                     /gene="rhaA"
                     /locus_tag="ACG8NF_000140"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0470660.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34014..35474)
                     /gene="rhaB"
                     /locus_tag="ACG8NF_000141"
     CDS             complement(34014..35474)
                     /gene="rhaB"
                     /locus_tag="ACG8NF_000141"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0470661.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35627..36496
                     /locus_tag="ACG8NF_000142"
     CDS             35627..36496
                     /locus_tag="ACG8NF_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0470662.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36622..37521)
                     /locus_tag="ACG8NF_000143"
     CDS             complement(36622..37521)
                     /locus_tag="ACG8NF_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0470663.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37686..38510)
                     /locus_tag="ACG8NF_000144"
     CDS             complement(37686..38510)
                     /locus_tag="ACG8NF_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0470664.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38521..39816)
                     /locus_tag="ACG8NF_000145"
     CDS             complement(38521..39816)
                     /locus_tag="ACG8NF_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0470665.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39832..40311)
                     /locus_tag="ACG8NF_000146"
     CDS             complement(39832..40311)
                     /locus_tag="ACG8NF_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0470666.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40317..41345)
                     /locus_tag="ACG8NF_000147"
     CDS             complement(40317..41345)
                     /locus_tag="ACG8NF_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0470667.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41362..42468)
                     /locus_tag="ACG8NF_000148"
     CDS             complement(41362..42468)
                     /locus_tag="ACG8NF_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0470668.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42697..45294)
                     /gene="adhE"
                     /locus_tag="ACG8NF_000149"
                     /gene_synonym="adhC"
     CDS             complement(42697..45294)
                     /gene="adhE"
                     /locus_tag="ACG8NF_000149"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0470669.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45599..45979)
                     /gene="yajC"
                     /locus_tag="ACG8NF_000150"
     CDS             complement(45599..45979)
                     /gene="yajC"
                     /locus_tag="ACG8NF_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0470670.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46076..47221)
                     /gene="tgt"
                     /locus_tag="ACG8NF_000151"
     CDS             complement(46076..47221)
                     /gene="tgt"
                     /locus_tag="ACG8NF_000151"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0470671.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47439..48137
                     /locus_tag="ACG8NF_000152"
     CDS             47439..48137
                     /locus_tag="ACG8NF_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0470672.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48305..50944)
                     /locus_tag="ACG8NF_000153"
     CDS             complement(48305..50944)
                     /locus_tag="ACG8NF_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0470673.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51563..52594)
                     /gene="queA"
                     /locus_tag="ACG8NF_000154"
     CDS             complement(51563..52594)
                     /gene="queA"
                     /locus_tag="ACG8NF_000154"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0470674.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52787..53961
                     /locus_tag="ACG8NF_000155"
                     /pseudo
     CDS             52787..53961
                     /locus_tag="ACG8NF_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            53965..54606
                     /locus_tag="ACG8NF_000156"
     CDS             53965..54606
                     /locus_tag="ACG8NF_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470675.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54603..55520
                     /locus_tag="ACG8NF_000157"
     CDS             54603..55520
                     /locus_tag="ACG8NF_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0470676.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55517..56188
                     /locus_tag="ACG8NF_000158"
     CDS             55517..56188
                     /locus_tag="ACG8NF_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470677.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56729..57217)
                     /locus_tag="ACG8NF_000159"
     CDS             complement(56729..57217)
                     /locus_tag="ACG8NF_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0470678.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57418..57684)
                     /locus_tag="ACG8NF_000160"
     CDS             complement(57418..57684)
                     /locus_tag="ACG8NF_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0470679.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57684..57947)
                     /locus_tag="ACG8NF_000161"
     CDS             complement(57684..57947)
                     /locus_tag="ACG8NF_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0470680.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58468..58608
                     /locus_tag="ACG8NF_000162"
                     /pseudo
     CDS             58468..58608
                     /locus_tag="ACG8NF_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58750..60573)
                     /locus_tag="ACG8NF_000163"
     CDS             complement(58750..60573)
                     /locus_tag="ACG8NF_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0470681.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61171..63876)
                     /locus_tag="ACG8NF_000164"
     CDS             complement(61171..63876)
                     /locus_tag="ACG8NF_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0470682.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64182..65129)
                     /locus_tag="ACG8NF_000165"
     CDS             complement(64182..65129)
                     /locus_tag="ACG8NF_000165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0470683.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65319..65588
                     /gene="rpsN"
                     /locus_tag="ACG8NF_000166"
     CDS             65319..65588
                     /gene="rpsN"
                     /locus_tag="ACG8NF_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0470684.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65764..66126)
                     /locus_tag="ACG8NF_000167"
     CDS             complement(65764..66126)
                     /locus_tag="ACG8NF_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0470685.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66137..67258)
                     /gene="alr"
                     /locus_tag="ACG8NF_000168"
     CDS             complement(66137..67258)
                     /gene="alr"
                     /locus_tag="ACG8NF_000168"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0470686.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67274..67624)
                     /gene="acpS"
                     /locus_tag="ACG8NF_000169"
     CDS             complement(67274..67624)
                     /gene="acpS"
                     /locus_tag="ACG8NF_000169"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0470687.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67754..69265)
                     /locus_tag="ACG8NF_000170"
     CDS             complement(67754..69265)
                     /locus_tag="ACG8NF_000170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0470688.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69587..70939)
                     /gene="murF"
                     /locus_tag="ACG8NF_000171"
     CDS             complement(69587..70939)
                     /gene="murF"
                     /locus_tag="ACG8NF_000171"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0470689.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71092..72168)
                     /locus_tag="ACG8NF_000172"
     CDS             complement(71092..72168)
                     /locus_tag="ACG8NF_000172"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0470690.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72364..74109
                     /locus_tag="ACG8NF_000173"
     CDS             72364..74109
                     /locus_tag="ACG8NF_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0470691.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74389..74826)
                     /locus_tag="ACG8NF_000174"
     CDS             complement(74389..74826)
                     /locus_tag="ACG8NF_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0470692.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74810..76183)
                     /locus_tag="ACG8NF_000175"
     CDS             complement(74810..76183)
                     /locus_tag="ACG8NF_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470693.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76505..76993)
                     /locus_tag="ACG8NF_000176"
     CDS             complement(76505..76993)
                     /locus_tag="ACG8NF_000176"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0470694.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77015..77617)
                     /locus_tag="ACG8NF_000177"
     CDS             complement(77015..77617)
                     /locus_tag="ACG8NF_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0470695.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22367 a        16547 c        14195 g        24549 t
ORIGIN      
        1 gaaaaaaatg taatcagatc attattttta tatgttgtaa cttcctgata agaaaactaa
       61 ttaaaagaaa gaggtaacta cataaactaa ttaaaaaagc taacttcata gttggaggat
      121 aaatggaaga aacttcaatg attgtttctc cttgtgttcc ttcaatcgac actagtccta
      181 ttatcaatct ctcttttttg tccatttact tttgcgactg ttccccaata atcaaataca
      241 ggaagcgtta ttttttgtcc tttagattca tgtactgtat agactatttt cgaatccatc
      301 acttgataag gcatagtatg ccattccttg ccaattctta cttcatgatt ctgcaaatgt
      361 tctttgtatt gtaatgcttc cgtgggtgca tagtcataaa gtcctccggg aaggagtgtc
      421 gtgtaacgaa taagattttc ttgattcatt accaagtgat ctctctgagt gacaacttta
      481 tgttcatagt ttgcttttgt tgcagcagat acagccaaca gtaataaaac aattgccgtt
      541 atattaacta cttttctttg cttagctgat gtgctcttca tgaaaacgaa cgataaaatc
      601 aacgagccaa acagtacttg aaagcctaag attcgccaag gaaattgaat catttgaatg
      661 ggtgtatttt gtaataaaga ccatggaaaa aatttggtgg ataagagcca agagactacc
      721 gcactaaggt aaatcacttt ttctttgcga ttaaatcctt taaaaaagaa gagtggacag
      781 atcaaagaaa taataccaat gattcctacc gtaaaggttg tcaggtcatt attcatcgca
      841 ccaatcaaac tatccgagac atttaatgct tggtgttgta aggtcgtttt atctggctca
      901 ttaatcgatt gaaccatgat ttgtcggaac atcggatacc aaaagtatgc tgttaaaaga
      961 atcgtcattc ctactgcctt tgacaactcc acaatcaacc ggatgctttc tttttttctt
     1021 gtttcactta ctacaaagcc gcctaagact agtaagccaa cggctattac actcataaaa
     1081 gtggaaagaa catgtgtata aatgagtaag ctcatactga ctgctaatgg aattgcacat
     1141 ggctttttct cataaatcag tgcgtaagca taatacataa ctatcggtat aaccgtaagt
     1201 gcaatcgctt cagcaatagc agaacgatga taaatatcaa tcgtacggta cccataaaat
     1261 gtatataaac agctaaatat aaaagccgca tcgattcttt ttaaaaattt tagtccataa
     1321 tgaaacatca gcatgatagt acaaacattc aatagccaaa catataagat ataactaacg
     1381 attaaattat tacttatcca ataaaaaatg actgccggaa aaaaggtcaa gtagggataa
     1441 aaataattca ctccgctccc ataatgatta aacgtcgtaa aattgattgg accagaaaaa
     1501 attgaagata accctttgac tctagcaaaa tggaaggcaa tatcccccga tgtagaaata
     1561 gtatgaacat ttctaaaata attgattgaa taaagtccag caataattgc taggagaatg
     1621 gacaatatta tttttttctt tacttgcatt acttaattat atactccttt catggattgc
     1681 tttatctagt ataacttaaa ttttaaataa atcgttattt atttttactc tcacttaaaa
     1741 aagcactaaa attataagag agggaggtaa tggtctgaac accaagaatt gttccaacat
     1801 cgaattgctt aactaatgag ttcaaagctt aattatgtgg ctcttatatt taccgagcgt
     1861 ggattgcgat ctctttagcg aaacaaacat ctttcataaa aaaaacagcc atatccgctt
     1921 tcttggatat gactatttac tactacttta ctatctttca ttcttcttgt ctcacttatg
     1981 ataaggcgcc cccgcattga tccggaaggc acggtagatt tgctcgacaa ggatcagacg
     2041 catcaattga tgtggatacg tcatttttcc aaaagagatc tgagcattgc ttcttttcat
     2101 gacggcttcg ctgagtccta aagagccgcc gataacaaaa acaaggtggc tttttccttg
     2161 gatacctaat tggtcgattt gtttagcaaa agattcactt gtaggatttt tcccttcaat
     2221 tgctaaggca tagacgtatt cttgttcttt gattttcgct aagatccgtt ccccttcttt
     2281 ttcttttact tgtctcattt gcgcttcgct taaattctcc ggggcttttt cgtcaggaac
     2341 ttcgatcaat tcgatttttg cataggcatt cagccgtttg acatactcat tgataccttg
     2401 gatcaggtat ttttctttta attttcctac tgagataatt ttgatattca tgttttctct
     2461 cctttggtct tcatgctcac tttgtttatt ataatcaatt ttttcgtcta tatgtttatt
     2521 ttggctattt ttgttaaaaa gatttcccca acagttatgc acaacatcca caggtttatc
     2581 cacagaaaac agtgttattt taccttattt catcacattc attaggcaaa catacgtttt
     2641 ataagaattt atccacttta tacacaggct gtggatatgt tttatccctt atttttccaa
     2701 tcctatccgc gtagttttac acagtatctg tggaaaaaga aaatcaaata ttttcatctg
     2761 aaaacaatga tttttcctaa agtttttcat aaaaagttca cagtctcaaa gtagactaaa
     2821 gtcaacaaat aagactatac taacagtaga taaatgaatg taattggagg aggaacacat
     2881 ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa aataatagcc tctggcgtaa
     2941 gttaggtctg gggcttgttg gagggatcgt tggaggacta ctgacagctg gcatttttta
     3001 tgcagcgatg ggcactggca acaatagttc tactgctaca agcggcaatc agaattctgc
     3061 cggtgaaaca gttgtggaaa atgtcaaagt caatgtagat tctgatatca ccaaagcggt
     3121 agataaagtt caagacgctg tcgtttctgt tatcaactta caaagtcaaa accaatcaag
     3181 cggttttggc ggactattcg ggcagcaaga agaaagtagc agctcagatg atagtaattt
     3241 ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata ctgcttatgt
     3301 agtcacgaat aatcacgtag tagatggcca gcaaggtctt gaagtcttga tgaaagacgg
     3361 tacgaaggta aaagctgaat tagttggaac agatgcttat tcagatttag cggtgttgaa
     3421 gatcaattca gacaaagtcg aaacagttgc ctcttttggt gactcaagtg ctctgaaggt
     3481 tggtgagcct gcgattgcga ttggttcccc actaggatct gaatatgcaa attctgtcac
     3541 ttcaggaatc atttcttcct tgaaccgaca agtgacaagt acgaatgaat cgaacgaaac
     3601 agtcaatatc aatgcgatcc aaacagatgc agcaatcaac cctggaaatt ctggtggtcc
     3661 tttagttaat atcgaaggac aagttatcgg tatcaactca agtaagatcg caagtacttc
     3721 tgaatctact tctaatgtaa gcgtagaagg aatgggattt gctattccaa gtaacgatgt
     3781 agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg acacgtccag ctctaggtat
     3841 cacaatggtc gacttatctg ccgtttctac tcagcaacaa gaacaaatct tgaagattcc
     3901 ttcatctgta actaacggtg tcatcgtgac aaatgtccaa acagctacac cggctgaaaa
     3961 agctggattg aaacaatacg acgtcattac taagatcgat gacaaagaag tttcttctgg
     4021 tgtagaactt caatctgctt tgtataagaa aaaagtggga gattctgtca aaatcactta
     4081 ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg acgatcgatc aatcggcatt
     4141 aaaacaaaat caaaatagtg aaaattaata aaaaagaggt tgtgacaaag acttgtcaca
     4201 acctctttat tttggaaatg cttctccgac tacggcatcc tcaggcaata ctaagattcc
     4261 tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca ttccatagct
     4321 atctacccct cgcaaagttc ctggccagat catcattcca tctggcatca tagctcctgg
     4381 ttttgcaacg acaacttttt gtccagcttc gatattgggt gcaccgcaaa cgatttgcaa
     4441 tactgtacca ttgtctactt ctgtctcagt aatcgataaa tggtcagaat caggatgtgg
     4501 gacgcatgtt tttacgtacc cgacaacgat cttcggttca tgatcagctt caagctgttc
     4561 ttcgaatcct gcttctttga tcaatcgatt caatttcgct aattcctcat ctgttaagct
     4621 tacttggccg ttcccttcga ttgttagatg atcggagata tggaaaaagt tccaagcaac
     4681 aacttgacct tgttcatctt tgatccgagc aacattccat ttgcgttcaa cagtgttttc
     4741 tgttccttta tcgtcggcta taataaccat caaggtgtct ccgacgtgtg ctttgttata
     4801 agcaaaaatc atgttctttc ctccattatc ctttgctgag atttatctta acaaaaaatt
     4861 gcattcactg catgaaattt ttttcatgct ttatttccga agaagcctct tctggaacac
     4921 catttattcg taacaaggga ctgtgacaag acttttgtca cagtcccttt actgttttag
     4981 ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa ttacttggct gttcttcttc
     5041 gtcagaaacg atcaagacat cacaaggtga atgacgtatc acgtagctgg ctacacttcc
     5101 tgtcataaag cgttcgactg catttagacc tgactgccca accatgatta gatcgatctc
     5161 atattttttt ggcaatttga ctgccatgga ttcacgaaca gaaccatatg caataattcc
     5221 ttctatattt gtgtaatcaa cagatttccc atattcttta cattcttcga tcatttcctt
     5281 ggctgtttca gtttgctgat cgactagatt ttgatcaata ggtgcatagc ccataaagtt
     5341 ataaaattgc tgatcgatta cgtttgctac atatactttc ccattattac ggcgagccac
     5401 ttctaccgct tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac ctactaaaat
     5461 attcttatat tcttgattca tcttgacccc tcctaacaga tgtcagcctt ttgttacact
     5521 ttctaggaac gattcgattt cctgttttgt tttacgatct ttattcacta gacgacctaa
     5581 ttcttttccg tcttggatca cgacaaaact tgggatacca aaaatgttcc attcagcggc
     5641 tacatctatg tacttgtcac ggtctacttc aataaattgc caatcagaaa agtcattttc
     5701 aatttcaggc atttgcggtt tgataaaacg gcagtctccg caccagccgg cagtaaagaa
     5761 aaatacactc tttccagttt ctacgtagcc tgctaattct tctaaactat ttgggataat
     5821 cattctttgt cctctacttt ctgtttatgt atttttcaat taaattatac acctttcttt
     5881 cgaactaaca aacattgatc ctctttttat aaaaaaaatc tggcaaaaag caatcttttg
     5941 ccagatttta tagtttcact atcgcattat tttccgtata cgatcgtatt tactgttgat
     6001 ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa atcgcgaata
     6061 ctgaacatcg tttgatgcgt atggatataa cgtcccacta cgccaatcac tgtacttggg
     6121 attccttcgt tttgtgtatg agcagcacct gcatctgtac cgccttttga aacaaagtat
     6181 tgataaggga tattgtttgt ttcagcgata tccagcaagt actcgcgcaa acgaggaagc
     6241 atgatcaaac ctggatcttg gatacgcatc aatgtacctt ctcctaaatg tccgaatgtg
     6301 ccatttttcg tcactgtatc atctgctgca gagcagtcta ctgcaaagaa cagatctggc
     6361 ttgaatttat tgacagaagc tttagaacca cgcaagccta cttcttcctg gacattcgcg
     6421 cctgcaatca atgtatgacc taatgtttca ttttgcaacg cttctaaagc ttctaatacc
     6481 attgtacagc catagcggtt gtcccatgat ttactgataa tattctttcc gttggctgtt
     6541 ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa ggacaccgta actcattgcc
     6601 tcttctcttg attcaaaccc agcatcgaac aaaatatcgg ttacttctac tgatttttgt
     6661 ccgcttgttc ctcgcaataa atgtggggga acggaagaag aaatacatgg ataattctta
     6721 ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt ccagccgcct
     6781 agaggtacga ctttaaaaag cccgttttct tggatttgtg tcagcatgaa tccgacttca
     6841 tccatatgtg ctgcgaccat tacacggggt gcatccgctt cttcatgatg gcgcagacca
     6901 aaaatgccgc ccaaaccatc taactgtaac tcatctacca gcggttccat atgactttcc
     6961 atataagcgc gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt taactctttg
     7021 attcgttgaa atgttttttc ttccatatgc tattcgtcta aatttcagac taaactcctc
     7081 tcagacttat attgatctgc ggataaaata tgattttttc tgcagaccga tcgtatttat
     7141 tatagcttat tttttctggt agcggttatt tatttcaaag aaaatatgag agtgtggtaa
     7201 aataagcaca aggagtaaca agtgagaaga aggaggtatg tccacatgta tgaagaaagc
     7261 gaactaagct attttaaagg cgggttagca gttggaatcg gtctaggatt attgagcggg
     7321 gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt cagcagacca agtacttgaa
     7381 aatatcaagt catcttttct aaaagaaggt gcaatcgaag gttcttggat ctcttttgaa
     7441 aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg tctttctcga
     7501 atagaagatg gcgaaactgt ttattatgaa tttttagcag atgctcatac aggaaccgta
     7561 ttagaaattt caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt gagagaacga
     7621 atacgcaccc actattcaaa agagaggtat catgtccttc ttttgtagtg ggtggttttt
     7681 tagatagagc tttccaaaca tatattccag tattttcttt ttaaataaat gatatttagc
     7741 gggaaaaact agcatttcct ttgattatga ggtattttta atttagcttt tgttttctat
     7801 agttaaggag agtgaaaaat ggaacaacaa gaaaaaattg ctgttctgat tccttgttac
     7861 aatgaggaag caacaatttc aacagtcatt gcagacttta aacgagaatt accagaagca
     7921 gatatttatg tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggt
     7981 ggagcaatcg taaaaaaaga acctcgtcaa ggaaaaggaa atgtgatccg ccaaatgttc
     8041 tttgatatcg atgccgacta ttatttaatg gtagatggcg atgatactta tcctgcagaa
     8101 gctgtacatg gtttgctcga gaaattacgt tctggtgaag cagatatggt cattggtgat
     8161 cgcctatcaa atggaaccta ctttgatgag aataagagac ctttccacga ttttggaaat
     8221 aaccttgtta gaaatacaat tactagaatg tataaaacaa aaattcttga tgtcatgacc
     8281 ggctatcgag gattcaatcg tatctttgtc aaaagttttc ctattatgag ttctggtttt
     8341 caaatcgaaa cggaattgac gatccatgct ttagataaaa aattcaagtt cgttgaaatc
     8401 ccaatcgatt atcgtgatcg tcctgaaggc agcgaatcaa aattgaatac attctctgac
     8461 ggattcaaag tgatcatgat gattattaaa atgtttaaag attataagcc tttgcttttc
     8521 tttgggattt ggaccatctt cttcttttta tttggattgg tcacggggat tcctgtgatt
     8581 cgcgaatata tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga
     8641 ttgatgactt tagcacttct ttcattagta acagggttga ttttggatac agtcgtcacg
     8701 aacgctaaga aagaatatga attgaatcta tatcatgttt atcatgaata ccacagtaga
     8761 aagtaaaaaa aggatcaaga ctgcaaaaat aatttgcaat tcttgatcct ctttttaatt
     8821 aattatttcg ccaaacgata gatggcatct gcatagattg ctgttgctct gaacaagtca
     8881 tcaagactca taaattcgtt tgcttgatgc attgtatcag tatatcctgg gaacatcgct
     8941 ccataagcaa cgccacgttt caacaaacgt ccgtaagttc cgccaccgat gatttgttcg
     9001 tagccttttt ctccagtgtg gtcttcatag acttgcaata atgtagccac caatggatca
     9061 tccattggta cgtaatgtgg ttccattacg cgagcacctc gagtaactgt tgcgccttct
     9121 tgccccactg tttgttcgat gtcactttgc aatccgtcta ctgttacacc ttttggaaaa
     9181 cggaaattca tattgatgaa gttcgcttcc tctgtaccat tttcttcaaa agcaaatagt
     9241 ccagcattca ttgtcaattt gcccattttt tcatcttcat aagcgactcc tagtttttct
     9301 ccataaaaat cttcatggac ataagcagct gctacatgga taaattgttt agcagatcct
     9361 aggaattcat agttatctaa gaacaatgcc aagaaagaac ctgcattgat accagattgt
     9421 gggcttgcac catgtgcgcc tttaccaact aattcgattt ttacatacgt attgtccgct
     9481 tcaatcgttc cgcttactgg atttgcttcg atgaattggt aaaatgcttc ttccatcgcg
     9541 atagctgcat ctgcacttgg tacttgaagt gctgcagttg ctgttcctgg caccatattt
     9601 tcgcgaagtc cggaaacaaa agatttcaag acataatctc ctgcattgtc gcctttgaag
     9661 cgtaaagcta aagaaacatt tcctttttca ccattgatga ttgggaattc agcatctggt
     9721 gagaaaccaa aatctggttc ttcttcatgt tcgaaataat aagccatatc gccccagccg
     9781 ctttcttcat cgctgccgac tacgaagcga actttttttg atagtttcaa gtcaagttct
     9841 ttaatgatct tcatcgcata gtaagctgcc atacttggac ctttgtcatc gcttgatccg
     9901 cgtgcaaaga tctttccatc cttgattact ggttcataag gatctgtgtc ccagccatct
     9961 cctgcaggaa cgacatccat gtgtccaaat atccctagcg tttcgtctcc ttcgccaaga
    10021 tcaatatgcc ctgcatagtt atcgacattt tttactttaa atccatcacg ttcgccataa
    10081 gctaacatat gtttcaatgc gtcgcgaggt ccaggtccaa atggtgcatc tggtgttact
    10141 tttgagtcat cgcgctcgct gttgacacgt aaaagatttt tcaagtcttc aagcaagtct
    10201 tctttacgtg cttctacttc tttttgccaa tcaattgtca taaagttaat cctccactta
    10261 tttaagtctt cgtgttcatg ataccacaaa ttacagaaaa aaatagttca aaaaatctga
    10321 caattccttt tcttatctgt ttttctctaa aaaaaatata cgctataatg agtaaaagga
    10381 ggtttttaga tgctgataaa cgaacaacta gtgaaaagag tcatcacacc tcgtccaaaa
    10441 atttcacata aaggaacatt tggacgtatc gttttgattg gcggcaatga acaatatggc
    10501 ggagcaatca ttatgagtgc tttagctgcc gtacacagcg gagcgggtct cacgactgta
    10561 gtaactgatg aaaaaaatcg gagtgctctt cacagtcatc tgccagaagc aatgattgta
    10621 gtttggacgg atactgacca gtttctttcc gtaataaagc aagcagatgt actcttgatt
    10681 ggtcccggat tgggtacttc ttctaagagt ttggaacgtt tcaaacaagt actagaaaac
    10741 caatcaaaag aacaatggct ggtgattgat ggctctgctt taagtttact tgctgctaat
    10801 catcttttgc taccctatcc cgaacaaact gtacttacac ctcatcaaat ggagtggcaa
    10861 cggttgagtt ctctaccgat acaagaacaa cgggataaag aaaacaaaaa gaagcaacag
    10921 gaaatcggtg ctgtgctcgt tttgaaaagt catgagacaa aaatttatgg tcaccagcac
    10981 ttctacacca atcctttagg ttctgctgct atggcaacgg gcggcatggg tgacacttta
    11041 gctgggatga tcacaggatt tttagcccag tttcctcaaa atgaccaaac gattgcagca
    11101 gctatttatc ttcatagttt cattggagac cagctggcaa aagaacaata tattgttttg
    11161 ccgacacaaa tcagcgaagc acttccgaaa tggatgaatt atttttgtaa aaaatcaatg
    11221 gaaaacgagg catgagtcat ggagaaattt acaggttatg tgcgagaacc tttttattat
    11281 gaaacagatc aaatggggat tattcatcac agcaattata tccgctggtt tgaagaggct
    11341 cgggtagcct tattggtaca tctagggttt ccctataaaa aaatcgaaga atctgggatc
    11401 atcatccccg ttctggaagt aaattgcagc tataaagaaa tgatccatta cggagaaaaa
    11461 gtgagcatcc atccaactat ccaaaagtat acggggacac ggttggattt tagctacgaa
    11521 attgtgggaa ctgaagacaa aaaattaaaa actactggca gtagtaaaca ctgtttttta
    11581 ctttctgatt cgcaacgatt agtgaagctg agcaaagtca atccggagtt agatcagtta
    11641 ttccaagcgt acgcttcttt ggatgatgta aattcttaat aaagaccgtc taagcatttt
    11701 tcttgtattg tgtcgagtag ttcgtataat gaaaagaaaa aagcaggtga tccccatgta
    11761 tgtcgttaaa gtaatgcacg gttacatcga taaaactggc tgtcgcactc gggaaaaaaa
    11821 cttagacaat ctgctcattt tcaaagacaa aaaagaatcc gaagcttttg ctaaaagaat
    11881 cggcggtcgt gtcaaaccga tccaagaggt acgaccagat tgattcaata tttttctttt
    11941 cgaggctcta ataggcatgc ctatcagggc tttttttgca gactgtgttt ttatgcccac
    12001 acgatttgta agcgatttat gttataatag attatatctg catagaaagg aaggttaaat
    12061 ggttatcaat aatttgcaac agcaagctta tgaatctata cgtaaacaaa tcatttattc
    12121 cgagttagca cctggaagta aaatctccga taaagtgtta gaagaacaac tagctatcgg
    12181 gcgtacaccg attcaagaag cactgatcca actgcgcaat caagagcttg taaaaaccat
    12241 tccacagtcc ggcacttatg tttcaaaaat cgatatccga tctgcttcat tagcccgtta
    12301 tacgcgggaa aagttggaaa aaccgatcct gcaagaatgc agtgcgaaaa tgaccaagca
    12361 agatcaagct aaactagaaa aaatactaga acaaaccgat caggcagtaa tcgctcaaaa
    12421 taaaaaagct ttttttgatt tggatactga ttttcaccga acttgttatg aaattgctgg
    12481 aaaacgagaa atttgggatt ggctagaaag ttatagtacg cacttgaacc gtttcagatg
    12541 gttacgtttg acgatttcag aattagactg gggtcgagta ttagatgaac atcagacaat
    12601 gcttcaatca atgattgatc ataactttga tgaagtcgga tttctttgca cgatgcattt
    12661 acatatgatt atcgaggagc aagaatacgt gattcataat taccccgatt attttgaaga
    12721 tattcaggac agacccatca aataagaggt tttggaatca gcgttttgac ctgagtatta
    12781 gagaaagaaa cggcaaaata gttcctcata ttttgccgtt ttttttgagc taataccgag
    12841 ggtcagctga ttgcacaccg tttattgaga ggttgtgaaa aagctgccct gcatcaagta
    12901 ataagaacag ccaagtaaaa atagcttctt atgtttttaa cttggctgga cttattaccg
    12961 cagatgcaag cttttgaaca ccgttttcca agaggtcgtg agcctgccgt tcatctccga
    13021 gtcacaagga acaatttcaa aaaacagctt ctcatgtttg ttgaatattt tgacttgtga
    13081 ccgaggagtt gacaggagaa catcgtttat tcggaaaaaa aggactgtga cacgactttt
    13141 gtcacagtct ctttatttgt aatctaaatc atactccgta catctttttt ctattttttt
    13201 tatttcttct aagatccctt ggctacgaat ttcattatat ggccaaagta agactgcctc
    13261 taatacataa ggcgaaaaat cgctttgatt catctctttt gaaaaactca tcgctgctat
    13321 ctcgctcaat gcacgaatgt ttgattgatg tgtataaaaa ggcaaccgcc acagcacaat
    13381 tttttcgctt tgcgtataaa ttcccttaac agaagcttcg atgtggtgaa atagttcatg
    13441 tccaataatc aaagcttgcg ctgtttcttt agagatcgaa gctagtcctg aacattttaa
    13501 tttttctaac ggctccgtca tcaatcgaat cccttcatct tctgcataag ttgcaaaagt
    13561 gattcgttcg tttatttgct ctgattcaca ggaaacgatt ggaacagcca tttgttcaca
    13621 aatttctcct acatcggcaa aagggtactt ttttttgatg ctacatgccg tttcgatacc
    13681 acaatcgatt gattgttgta taagaatttt ctgcttggac aaaggaatct ttttccaaaa
    13741 taaatctcgc cgaaactggt agaatcccca aatttcttcg gggatctgtt gtaattcgtt
    13801 tagtaactct tctaacttat gtttgtttga tatttccggc aacgacgatc acttcttcct
    13861 ccggttccat agtagatact tccagatcca tcaaaagctg tagttgttcc agatccgtcg
    13921 ctgtaaaatc agagactctt gctcctaagc agagataaaa ttctggtcgg gcaatcaatt
    13981 cagtttgata aactgccata tcttcccaaa gttgttcgac agctgtcata taattggctg
    14041 cagttgtttt catacatgaa gcatgtccac gttggacctt gataaatcct ttttgatcga
    14101 tgatccgaag acttccagca ttttttgaac gattctgttc gccgcataca taaaagaaag
    14161 gtgtattttc caataagcaa acttcatttt tatttaatcg catatcctct gcagcaagct
    14221 ctagtgcttc ttctgttgtg atctccttgg ttagatcggt tgctttcact tccgtagaac
    14281 cagtagcgat agccgttact tttgaagtct gtggatcgat ttctacgtgg acttcgatcg
    14341 attctggtgt agcgcctgat tcaatcgctt tattcatcgc ttcatttttc aacgatcgta
    14401 tatcttcttt acttggcgaa gggatgatac gttctacaac atctcggacc attgcaagtg
    14461 ctacaccaat agaagaaatc acttcggcgt tttcaggaat cgaatatttc acacccattt
    14521 tattactaaa gtaagtaatc aatgaggcag cgccgccccc tacacctaca agagaaatct
    14581 gatctttctc caagtgatac ttgtctgcca attctaaaat caccggttca atcttagcat
    14641 aggatttttc catgatctgt tctgcgatgt cttcaaccgt cgtatgacaa taatcagcta
    14701 atgcttggat ggcttttcgt gcagatggga catttccata agaaaaatgt tcttcttgca
    14761 ctaatccaag gacattggct gcacatgtat ttgtaatagt cacttcttct ccgtcttcca
    14821 tcactacctt gacgtaatca gcagggtctc cttctttagg agagaaaaat tcaaccttag
    14881 gacctttgat tttttctgtc tccgtaaata ctgcataatc tagaccggca atatgtgcag
    14941 aacgtggacc gacatcgatg attcctgact gattagcccg taccatcgaa ccgcctgcca
    15001 cacctagaac ccgaacatct aatgaagaga tatatgttgg gtggccacct acaattgaat
    15061 aatcgattgc tggacgtcca tttttaatga ccccgatatt ggttgttgtt ccgcctactt
    15121 caaaataaac accattagac gctctcaagt acatcaaaga gcccatgact gatgctgctg
    15181 gaccggaaag catggttaaa acaggtcgtt ttttcatttc attgatttcc attactccgc
    15241 cgtctccgcg cataatcatc aaagaaacat tgacccctgc ttctcgtacg gaatcttctg
    15301 tagaggttgc cgtatctagc atttttggta aaatagatgc attgattgct gctgtccgag
    15361 ttcttcttgt caatccatat aattttgtga tgtcagaagc catcgttgta gggatcgatt
    15421 ttaccgatgc tgcttcataa acaactcgtt ctggttcccc attatctact ccaaatgcca
    15481 tagaagaaac caagacctcc gctctttctc tttctaaaga agaaatcgtt tctgaaacac
    15541 ggtctacatt taagtgtttg acaggtaaga atgcattgac aatctctatt tttttcttat
    15601 tccctaaatc gatatcgttc aatctagttt gtcgttttgc taaaaaacct tctaatccac
    15661 cttttgccat cccgattacg ccgacttttg ctacgtctcc ttcaatcagt gcattagtcg
    15721 cttgtgtggt agaatgtgca acaaacacaa catcttctgg actgatatta ttttccagca
    15781 aacaattttg aaaagactga acgacacctg cggctactcc acggacatca tcgtgggttg
    15841 ttttcactga agattttccg atgatttcgt gcgtcgcatt atcgattgcg actgctttcg
    15901 tatgtgtacc gccaacatca attcccattc gcacaaaacg tttcattttc ttttcctcca
    15961 ttcatctaat cgtttttatc gacgttttgt ttactaggcg ttcccgtaca ataagaacgt
    16021 gatcaggcat aagatcgctc cgatgatcca accagttggg atggatttct tcatatattc
    16081 tcgagaagtt acttttgtat aacctaatcc ccaagcaacc caagattgtg taatatctaa
    16141 atgctgagga gcaatcgttg tgattgcaaa taacgggaac aaaaaggctg gcgacatcgt
    16201 tggattaaca gctaatacga cagctaaaat agctgagcct gaaccgacta gattcattgg
    16261 accgcggaaa aatcctagtg gtgtcaatat agcaaatacg attgccaaga ctaatggtga
    16321 ttgcggtatc caatctccaa aaatagctga aaaataagga gaagcatacg cagctgaatt
    16381 attgaacatc gctaaggtca tcaagaaacc aaccattggc gcaacatcta ctgcgccatc
    16441 tgtaaataat ttcgcaaact gacgacagat ttctttataa cctccgcgca attttccagt
    16501 aaacagcaat gcccagatac ctgctagaat aaaacctaag atgattggaa gatctaaaag
    16561 aacgacccct aaaactggta ataatacggc taaccatgag atcattggtg cgtcgccacc
    16621 ttcagctggt acgtttgctg ccattgcata gcgtttcttt ttgttcattg atatattagc
    16681 gaccgttagt accacaacta agcttacaat catcccaatc ataccaattt ggaaatagtc
    16741 attataagta tagctttcag ccgctggatt gaaaccagca taaatcgttt gatattgttt
    16801 gaaattgacg atattagcaa agattccagc cataatcgag cccataaatg agaacagtgc
    16861 aaccggtact gaaataccta atgacatcaa gattggcaac acaataactg caatagaaat
    16921 cactggtccg attcctgtca tcgacatgaa tataacagcg gttacaatac atagtaagct
    16981 catcgtgatt cttggcttgt cccctccaag ttcaactacc tttcgaatca gggtagctgc
    17041 gattccgcta tctactagaa cacgcccgaa aaaggcacca aagaagacat ttaccaagat
    17101 cgatttggcg tactcagctg gccccgtcgt atagatatgc gtcaacacat caattactcc
    17161 ttgcccttcc atcgccgggt ttggtgaaaa cgtgtttccg actagcgcta atcccatcca
    17221 tacagtagcc atgatggcaa agccgaccat caagttgtag ccttttacac agtaccagac
    17281 catgaaaaag aatgagataa ctaacaaaat accaataata atatcactca tgttattcct
    17341 ccgtccattt tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta
    17401 tgtagttagc gctttcatta aagggtgacc ataaatgttc agttttcaat taaaaaacct
    17461 taattatgct attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa
    17521 ataaaaaaag aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta
    17581 gcttatttag ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg
    17641 ttaacagcca agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg
    17701 tgcattaatt ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc
    17761 cacaatttac ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa
    17821 ttcattactt gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt
    17881 gctaatttta gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc
    17941 atgtataatt cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt
    18001 catttttgct agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt
    18061 acgcaattga cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg
    18121 gaagcgtttc aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt
    18181 tttttgtttt ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct
    18241 gtccaacagc cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag
    18301 aacatgctgc gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct
    18361 tcagctaagc gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg
    18421 aaacggatag aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt
    18481 tttgtattga agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc
    18541 actttttgtt ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg
    18601 tagtccatga ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag
    18661 tttgcttgtt ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca
    18721 tctgaaccga tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt
    18781 gctatggtca ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca
    18841 ataaaaaaac gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa
    18901 actatctagc ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt
    18961 ctcaaccatc atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt
    19021 cttccttttc tgaaaatgat tagattagca gactttcttg cagtgattcc cgcttctttg
    19081 ctatactaaa gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct
    19141 cttcctcttc ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt
    19201 ctgatcaacg ggggtgacca aagctatctc gatgccaaaa atatggtatt aagagagagt
    19261 catatccaaa aacttacgat gcaaaaaaca gttttacgtc attttgaatg cagtaatgtc
    19321 atttttgaaa aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc
    19381 gtgtttcatc aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt
    19441 actttcactg attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc
    19501 tccttcgatc attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt
    19561 tttttgtctg acaatcagtt gaccggttcg aactggtttc gaacttctct gaagggatta
    19621 gactttacga ctaatttctt cacagcaatc gctttatctc aagattcttt ggcgggacta
    19681 atcgtcaacc aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac
    19741 tagatcaacg aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga
    19801 tgatcaaggc gattccccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc
    19861 cgaagaatcc tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt
    19921 tcatcgtgtc attcaagttg ttattcagca aattattata agtgccagag agctggtcta
    19981 aaatctgcga agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agcgcatcgt
    20041 ccaactcttc tttttccttt tctgacaact tgcgataaat agccaatgcc ttgatttgtt
    20101 ctaaaagtac tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat
    20161 ctgaaagttg cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct
    20221 gacgttcact attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg
    20281 tacgaaacaa taaactgtat acactcatgt ctggcattcg ttccaaaagc gatcgaatta
    20341 gtcgaatcac gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa
    20401 taaaagcgat aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg
    20461 atacaagcag taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat
    20521 ctagagaata agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag
    20581 ctagcacatc gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt
    20641 tttcccgcat actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa
    20701 ataaaggttt accctttatt tgagcaactg taaatcaaat ggctactttg gtttggcgaa
    20761 tctctgctaa tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca
    20821 caaaattata gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc
    20881 attgatcgta acgtctgttc aattctttgt aataatcgta tagacttgga tcaaaggaca
    20941 attgttcata cgaacgacca cgtttttcga tacgctccaa catcgtatca aaagatacac
    21001 gaatatgaac taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca
    21061 tgttttccag cagttcatca tacacacgaa cttctgtttc tgttgctctt cctagatctg
    21121 catttagatg gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat
    21181 ctttcattgc ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa
    21241 aagcatactg ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt
    21301 cataaaatgc ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg
    21361 caccaattgt acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa
    21421 atattgtgtt taactccaac ctttttaaat atacccccaa tatctagtag tgtcaattgt
    21481 atttttttct agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt
    21541 ctttagaaaa cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa
    21601 tgttttttga gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc
    21661 gctttatttt ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta
    21721 tggccaagga gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg
    21781 agaaccagcg attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct
    21841 ggatgcaatg gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc
    21901 tcatgcatta aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca
    21961 aaaaaacttg caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag
    22021 cgctgcagtc gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc
    22081 tcgtgaaata ctattagaaa atgtccagct ttcggaaaaa atcgcccatg gtaatcctag
    22141 tggaatcgat gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc
    22201 tttcgactac ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa
    22261 aggacaaaca agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga
    22321 aactggacaa atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat
    22381 tgaaaattca ccagaaacgt tggcacagac aatggatgaa tcacaatcac ttctggaaaa
    22441 gctgacaatc agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc
    22501 attgggtgcc aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac
    22561 aaaaacaaaa gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg
    22621 gatacaagga ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca
    22681 ttgctctaat caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca
    22741 gcctttcgtt aacattggat gcattttata cagagacaga agtcatcttt tctgatagct
    22801 atatggtaga tgaattttat ctagatggca ccttgcaaga cgaaaaagca acaaaaaaag
    22861 tcagtcagtt tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa
    22921 tcagtcagaa tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg
    22981 ctttagcagg cgcttgcaat actgctctta agcttggatt agatgatctc tctctttcaa
    23041 gatttgctcg acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgtcgaat
    23101 gggaaaaagg ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg
    23161 aagacgattt agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca
    23221 gaaatgggat gcgtcggaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg
    23281 ttgaagggga tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg
    23341 gagaaacgat ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc
    23401 cattcactta ctggtctcct gacagcttaa aagcgatgga tgcggtgcga caattgagaa
    23461 agcaaggcat tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg
    23521 aaaacagcca tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag
    23581 taattactgc acatgctggt ccaggaattg cgattattga ataaagggag atttttatga
    23641 ttgaagtatc tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag
    23701 gccatccagc agttatcgct gcagtcgatc aattcgtgac agtcactgta gaatctgcac
    23761 gaaaagtcgg aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac
    23821 gcaacggaga attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta
    23881 ttcgcttgac tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga
    23941 aagtaacaag tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg
    24001 ccgtgaccgt tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcagt
    24061 tggagatttt caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg
    24121 gcgacatcgc tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt
    24181 ggctccaaga acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag
    24241 gtctatccat tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg
    24301 gtagccctgc ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagataaaa
    24361 tggtggctta tcagcttttc ttaaaaaata gtacagaatg tgtcaacgaa atgatcaaag
    24421 ggtttaaaga aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac
    24481 tgcatgattt atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt
    24541 gtaatttagc tgaacagtat gaaggagccg caaaatcttc tggtgcaggt gggggcgatt
    24601 gcggaatcgt aattgttgac cagaaatctg gcattcttcc tttaatgagt gcatgggaaa
    24661 aagcagaaat cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat
    24721 gaatcgaaaa gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga
    24781 atttgacttc gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga
    24841 tctaagtaca caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac
    24901 tggcggaagc gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc
    24961 agatttgatg attgccactg gttcagtcag cgctgccttg aaagtccctt ctttagccga
    25021 tacttatacg attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc
    25081 agggacttct gtcgaaagag ccaaagaagc tatccgattg tttcacgcag atgccttaca
    25141 gattcatttg aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa
    25201 ttggaaagta cttattcaag aaactcaaac agccattgac gtccccctca tcgtaaaaga
    25261 agtcggtttt ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac
    25321 agttgatatc agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc
    25381 caagcgggaa ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga
    25441 agcaaatgaa gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta
    25501 tgacatattt aaagcccttt gtctcggcgc aaatgccgtg ggtacttcag gaaccgtact
    25561 gacgcatctg atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga
    25621 agagcttcgt ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca
    25681 atctctgatc ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt
    25741 gaagtatgga agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttgtctca
    25801 cgctctaaac acgtacaaac ggcggaagta aaagcaactc cttcggaaat aagctgaaat
    25861 tcacaaaaat ttgaaaagca attttcgtga attccttctt atttcttgga gttaaatgct
    25921 tctgtcccag cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt
    25981 tcaatccgct gatttcacaa cctctccact aaacagtgta ctaaagctct tttaaggttg
    26041 ttttccgata taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc
    26101 agatgcgcta gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg
    26161 tgctgctggc gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg
    26221 tgtttcgcgt aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca
    26281 ttggatattg tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc
    26341 ttttgccgca gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat
    26401 attgatgatt ttaccgccgc ctttttcgat catatccgga ataactgctt ttgaaacgat
    26461 gaatggggca tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat
    26521 catcggtgtc cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc
    26581 ttctttgatc tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata
    26641 accttttgca tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg
    26701 attcaagtta ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa
    26761 tccaatacca tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc
    26821 catactaaat tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca
    26881 tatccatgtc tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc
    26941 ttgtacctac acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta
    27001 caactaaatg ttttgtttca tctggtttgc ccatcatgtg gaacacacgt gtatcttcat
    27061 tggcataatc aaagtagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg
    27121 tattccaagc gcttcctggt tctaaaatcg tataacccat ttgtaattga cagctttcgc
    27181 atacatttgg atggatgtat tggtagattt tacgttcatt cagtgtcagg ccttcaccag
    27241 tctccattgg ttttacttgg tcgatactga tttttacatt tggatattta tgatgagctg
    27301 gaacagaact aatatagaat ttagctgggt tttcagcatc ttgagaagaa aatacaacat
    27361 gtcgtgtttc ttttccgata tagtatccgt cttgtttttt catttcttct ttttgcccat
    27421 cgatctcgat gaatcctggt ccaccgatat tgatcacacc tagttcacga cgctctaaga
    27481 agtaatcaac acctagttct ttgtctaatg tgatttctaa ttctttttca gtaggtgtca
    27541 ctccaccaaa gatcattcga tcgttgtgcg tataagtcaa actgattttc cctggttcaa
    27601 ataccttttc tactaaaaat tcttttctca attgttccgt tgaataatga cggatatctt
    27661 ctggactgtg tgtataacgt gtttccattt cttgcatttt cttctactcc ttctatcgaa
    27721 tgatttttcc agagccgcat gctaaaaatt gtgcgacttc ttcttgacta aattggttgc
    27781 agtccccatg gatcgtatgc ttcaaggcag aagctgccgt agcaaaatca atgatttctt
    27841 gcggagacat attggataaa atcccatgga gcacgccacc tgcaaaagca tctccgccac
    27901 ctactcgatc cacgattggg gtgattctat ggacttggga ttccacatat tcatggttca
    27961 tccacaatgt ccccttcaat tcattttcac ttgctgaatg aacggtacgc ttggtcgaat
    28021 aaaacacctg aatattcggg aacttttcct gcattttttg ataataataa attagaggtt
    28081 gttcttctgc ttctggcgct ttctcgatac ctaacaaata aagagcatcc atttttccag
    28141 ctgagcagat atctactaaa ggtaaaagct gatggattac ttcgctggct tcttgctgtg
    28201 tccataattt tccacgatag ttgatatcga agctgatcag gcagccagct ttttttgcag
    28261 cttctatcac ctgtttcgtc attttttgcc attttttaga caaagctggg gtgattcctg
    28321 aaacatgaaa tatatctatt ccttggaata actcctctag cgaccattcc aaatgatcca
    28381 tttcagcaaa acttgatccg gcacgatcat agatcactga ggcggcgcgt tcgccactac
    28441 cagtttccat ataataagta cctagtcttg atccaccaga taatacctgt ttcgtatcta
    28501 ctccataaca ttgcaagtgc tttttgactg cttttcccaa tgcgttatca ggtacttttg
    28561 tagcaaaaat cgcttcatgt ccgtaattag ctaaagaaat tgctacgttt gcttcccctc
    28621 cgccataatg ggcagcgaat cgttcacttg aatcgatacg ggttccctgc tctgtggaca
    28681 atcgcaacat gatttctccg agtgtcacta cttttcccat aagcagctac ctcttgattt
    28741 ctttcatttt tgctgcatac tgttgcgcga cttccgttac tttgtcaaaa tctcctgtag
    28801 ctgcgggtgc taaaaggttc ccacctactc cgactgttac gacaccagca tcgaaccact
    28861 ctttcatatt gtccagactc acaccgccag ttggcatgat attgacttga ggcatcggcg
    28921 ccttgaaggc agaaatgata cttggtccat aagcgcttcc aggaaatagt ttgacgatat
    28981 ctaccccgct tttcagcgct gttttgatct cggtaatagt catacatcct ggtaagtaag
    29041 gaatttgata cagattgcac atctctgccg tttcttgatc aaaggaaggg ctaacaatat
    29101 actctgcgcc agctaagata gccaagcgag ctgtgacagc gtctaatact gtgcctgcgc
    29161 caatcactat ttctggttgg tctttgtaga atgacaaaag ttctttgatt acttgatctg
    29221 cttgagggac tgtaaaagtc aactcaatac cagttagacc acctttgatg attgcgtggc
    29281 aagcattcaa agcttcttct ttgctttttc cgcgtaccac cgcaatcact ccggcgtttt
    29341 tcaatcgaga taaaatatca atttttttca tcttctccac ctatttccta tttagaaatt
    29401 ttattttata acttggaaat ctatacccat attatacggc agaaaagaaa aattgcaaac
    29461 gcttttttca ttatttttga attctttgct cgatttcgct ttttgtttgc ttgagggcac
    29521 tgattatttg ttcttttgtc tcatctgtta aacgatattt cggcatactt acactaaatg
    29581 caccaatgat ttgttcgttt ttcacgattg aggctcctat acaaaaaata tcacgttcga
    29641 tttcttcatc gtcaaatgcg atgtgggttt cttttaccgt tttgatttcc tttttcaacc
    29701 gtaatgcatt ggtgatcgta tattctgtgt aagggatcaa cggatgcttt tcaagataat
    29761 cagtgtactg ctcttcactg aattctgcta atacggcttt gcccatcgca gaattgtata
    29821 atggtcgggt gattccgatt tttgaagaca tgcggatcgt ttgatgtttt ggatctagtt
    29881 tgttcacgta caagatctca ttgttgtcca atacccctaa gtggatagtc tcatcgatct
    29941 tttgctgcag ctgttctaaa aacggtaaag tcaactctac caaatcgatt tgttcaatgt
    30001 ttttattcgc gtagcgaata agctttgcac ctaaacgata gtttttctct gaatttttat
    30061 ttacgtagcc gatcatcaac aaagtatcta gtattttcaa gactgtagaa gaggtcattc
    30121 ccacaccctt tgcgatcgtc tgcaaagaga catcttggtt ttccgctaaa tagtctagta
    30181 ttttagaggc tttgatcaat acagttccat aaggcttttt attttccata taatattctc
    30241 ctatttcttt ataagaaaaa ttatagcgga aataccatca atttaaaaga gatatccaga
    30301 tataaactac catttctctt tacaggcaat ttaatgttga agatccgctt cgtttttaca
    30361 tcaaactttg atataaagca atgatttctt ctactgtcgt ttctcttgga tttcctggtg
    30421 tgcatacatc tcgcaaagca tcttctgcaa ttgctggaat atctgcttct tttacaccta
    30481 attcagaaat ggtttctggg attccaaccg ctttgctcaa ccgatcgatc tcaccacaag
    30541 ccgcttctct tacatcttct aaagacattt cagctgctcc tttaatccct agaacaagag
    30601 cgatctcacg gtacttttct ccagtatatt ctttgttata tttcatgaca gttggcaaaa
    30661 gtgctgcaca cgctacgcca tgaggaatgt tgtaccatgc acttaacggg tgagccatcc
    30721 cgtgtaccag tcctaaaccg acatttgaga agcccattcc cgcaatatac tgtcctaatg
    30781 ccattttttc tcgcccattt tgatcgccat ctacagaagc ttccaaagaa tggccgatga
    30841 tttcgattgc ttttaaatgc agcatatcag tcatttccca agcgcctttt gtgataaacc
    30901 cttcaatagc atgcgtcata gcatccatac ctgttgatgc agccaatttt ttcggcattc
    30961 ccatcatcat atcgctgtcg atgaacgcga cgattgggat atcatgagga tcaacacaaa
    31021 caaattttcg gtgatttact tcatctgtga tgacgtagtt gatcgttact tctgctgctg
    31081 ttccagaagt cgttggtata gctaatatag gtaaacatgg atttttcgta tcagcaacac
    31141 cttctaaact aatgacgtct gaaaactctg gattcgtcaa aataatccca atggcttttg
    31201 ccgtatcgat tggcgaacct ccaccgattg cgatcaaaca gtctgcctct gcctctttaa
    31261 caaaagccac tccatctttt acattttgga tcgttggatt aggtacgatc ccgtcgtata
    31321 aagcataagc gattgctgct ttatctagaa gttctgttac ttttgttgct ataccgtgct
    31381 cgatcaatcc tttgtcagta atgacagctg ctttgccgaa tcctcgctgc tgaaattcag
    31441 ggacgatcga ctcaatcgct ccttttccaa aatacgatgt ttcatttaaa atcattcgat
    31501 tactcatttt ctattcctcc taatttgtat tatttttcac aataaaaacg aaagaacccc
    31561 aagcaaccgg ttgcccagtt gcttgttgct acagttcaaa gactggcatc aattcccaac
    31621 tgactggaga ggcatctgga tttgtctcca tgatatcttc catgtacttc caccattttt
    31681 gattgataga tgtatttgcc attttttccc ataacttttc atcttcgatc actaagtaac
    31741 caaataacaa acttgtactc ggatcaagat agatcgaata gtcgatgacc ccgtgttcac
    31801 gaagtgctgt acgcatttct ggccaaagtt catcatgtct ttttttgtat tcttcttgtt
    31861 tttctgggta aactttcatg caaaaagctt ttttcaccat gtcagacacc tgctttcgta
    31921 tgaacttcct ccaaataacc tgcttttgga gtgacaccaa acgcatctgc taaatgccac
    31981 aggttttcgt ccgtgattgt ttgcaagatt ggtccttgcg ctttgacgta cgtatagact
    32041 tctgctgcct tttctgctgt ctcgatcaaa ccaaagactt catccatgtc ttttcctgac
    32101 ccgtagattc catgttgtgg ccataaaacc aatcgagttt ctttcatttt ttcagctgtt
    32161 gcttccccaa tctcatttgt tcccggaacc atccacggaa tgatggaaat tccttctgga
    32221 aaaacaacta agcattccgt acacatttgc catagcgtac gactaaagga acgttcgtct
    32281 aattcatgcg taaaactcat cgctaataga tgactagcat ggcaatgcat aacgatccga
    32341 ttatctgggt ctacttctaa acgggcgata tgactcatga aatgacttgg caactcgctt
    32401 gtaggaaggc attcgttttc cagcccccac aaaagttcca gtgttttgcc atcttcagca
    32461 actcgaacga ttccaagatt ctcggcaggt tcgctcgcta catttttaaa atatcttcct
    32521 gaaccagtta caataaaata gcatcctgct aaacggctgg catcaaaaat catcgagatg
    32581 cggcggatca ctttttcagg atccaagaac ggtgtgatct cttcttcttt tagtaaataa
    32641 gaaatattcc cgccatttct ttcatcccat cccaagcgat aaagattcga cgtcgtttcg
    32701 atcatttctt tcacataagg cgcttgtaaa atatctattt ttttcaatat agccacactc
    32761 ctattttctt aattccaaca cgtctttttg atattcctgg acttcttcca gccaatccaa
    32821 accaactgga acttgatttt tgtaacagaa ataattccat acatcggcat acgggaaatc
    32881 tttcaattct tccgtataag ccattcttga agtaaagtca aaagccagct ccatttcttt
    32941 caatcgctca actggttcca gcatagcttt cagcagtgct ttttgtgtat ttctcgtacc
    33001 gatgacccaa gcagcgatac ggttgatcgt tgcatcaaag aaatccagac cgatatttgt
    33061 tttttccaac aattcgttac ggactaattc tttagcgatt tcctgcaatt catcatccat
    33121 caaaaccaca tggtctgaat cccaacgcac tggacgggaa acatgcaaca tgattccttt
    33181 gctgaataaa gctaatgaag ataatttatt ggaaatcact tctgttggat gaaaatgtcc
    33241 agcatctaga caaacaagtt tgtcacgagt caacccgtaa cccatataaa attcatggga
    33301 accgactgta taagcttcag cgcctaaacc aaatagtttg ctttctactg catcttgtac
    33361 ataccgttca tctaactctt ctgtaaaaat ttcatcgagt gattccatta aacgttcccg
    33421 tggggtaaaa cgatcaatcg gattatcttt catcccatca ggtacccaaa agttattgat
    33481 acttgtttgc ccagtttctt tcccaaagta ctccgcgatt ttgcggcttc ttttcccgtg
    33541 ttcgatccag tattcacgaa cttctttatc tggatgagcc aatgtaaagc cgtctttcat
    33601 cattggatgc gaaaagaaag tcggattgaa gtcaagaccg atttcttctt tttttgccca
    33661 ctctacccat ttttcaaaat gtttcggctc aatttcattt agatcgacag attcttctgt
    33721 atccaaatag atagcatgaa ggttcaattt gtgtttgccc ggtatcaacg aataagcctt
    33781 ctccagatct tgtctaagct cttcaggtgt tcgtgcggct cctggaaaat tccctgtcgc
    33841 catgattcca ccggtcaatt cgccatccgg tgtcaagaaa cctttgacat catctccttg
    33901 ccagcaatgc atagaaattt tgatgtcctg tatcttttct agtacagcat ctgtatctac
    33961 accgatagtt gcatattttt cttttgcttg ttcgtaattt ctttcaatcg tgttcatacg
    34021 attactcctt tgattttatt cacgtaccat tccatcgtgt tttgcttatt ttcatctggg
    34081 taaaaagttt cacatggaaa cgactgtttg attacatttc ttgcttgttc tatattcgaa
    34141 aactgacctg tactgagcat ttgcatggca acattcccga ttgctgtggc ttcactaggt
    34201 cctgcttcta caatgattcc ggcataatct gctgtcaatt gattcagtag acgattattc
    34261 gatccgcccc ctacgatgtg cagcttttcc aatttcgttc cagttaagtg ttctactttg
    34321 atcagctctt gtgcgtaaca aagagcaaga ttatcataca cacaacgagc gatagctccc
    34381 gatgagacag ggactggctg ctctgtttca aaacagtatt gctgaatctc ttctatcata
    34441 tttgggggat tcaaaaatcg agggtcattg atttccatca cttgctgcag cggtttttct
    34501 tttgcagcaa gctctgccaa ttccgcataa ctgtactcgt actcttgcat tcgggcaact
    34561 tcctggatca accacatccc catgatgttt ttcaaaaaac gcaccgtgtt atgggcaccc
    34621 cattcattcg tataattttc agaaaaagca ttgcgagaaa cattggcgat ctttgtttca
    34681 atccctaaaa gcgaccatgt cccactgctg atataacccc aacgttctcc ttgacctggc
    34741 gttcctaata cagctgacgc cgtatcatga cttgctactg tgataaaggt cacatctggc
    34801 aaatcatatt ttgaaaattg ttctttttgc agatctccta gtatcgttcc cggctctgtc
    34861 aatggtggaa ataattcctt cgagacaccg attttttcaa gaagttctat ttcccattct
    34921 tttgtccctg cattcagcag ctgcatagta gatgcattag ttttctccgt caccatgtta
    34981 ccagtaaaga cataccctaa ataatcaggt atcagtaaca atttatgtgc ttttgccaaa
    35041 ttttcttttt cttcagcaaa caattggaac aaggtattga aaggctggat ctgaatccct
    35101 gtctgctcat acaacttatc tagagaatag atttcagaaa actgttctac tgctccattg
    35161 gtacgcttgt cccgataagc aatcggatca tttatccgat ccccttgttc atcaatcaga
    35221 cagtaatcga ctgcccatgt atcgatcccc gcaaaaaggc gatcaatccc tgtctgcttt
    35281 aatttttcaa atccaatcaa aatctctttg ataagataat cgatcttcca gcgatcgtga
    35341 ccgtctttgt tccgaaagcc gtttttgaac cggtgtactt cttccaagat caaagaacct
    35401 tttgcatccc tcgtactttt cattagtcgt ccgcttgatg caccgatatc gatggcaaca
    35461 aatgttggat tcatttctcc acctctttta ctttattgtt gattagcact ttattaataa
    35521 agcgttatca tttattgaca atgctatcat aatacagaat cttatatttt gcttcacact
    35581 attttggtat ataattgcat tatcttgaca agttgggagt gtttacatgg attactggga
    35641 aaaagccaac cactcatggt cggaagactc aaaaagagtg atcttgacgc ctacacaaag
    35701 aagcaggaat ttattttttt atatccaaga aatcggttac ttcaaagcac caatccctta
    35761 ctatactgaa cgagcacatt taccttctta cttgataaag tttactttgt ctggcaaagg
    35821 ttcgcttaca tataatgata atcagcaaca gctaaaagct ggagacttat tttttattga
    35881 ctgccaagaa tatcagcgtt atcaaacgat cagcgaagaa ccatgggaaa tggattggat
    35941 ccatttgaat ggtcagaacg tccaatcatt ttatcaggag ttcacaaaag acggccataa
    36001 caccttccat tcaacaggag atccatcaga aaacaagatc caccatttga tcacagaatt
    36061 gatccacatg caacaaggca cgaatgcccg taccgatttc cagtcctctg tagctatcca
    36121 cgagttgctg aatgaactga ttttgcaaaa gtatcagcta gatttcgaag aagaggatat
    36181 tcccgcctac gtttacaaac tgaagcaata cttagacgaa catttcaaag aaacgattac
    36241 acttgatcaa ttagaacaac aattcttatt gaataaatat cagctgagca aagatttttc
    36301 caaatttatc ggttctccac caatcgatta tttgatcagc aaaaaacttt cttacgctaa
    36361 agaccttctt cggtatacag aatttacggt tcgaatgatt tccgaagaaa tagggattga
    36421 gaattttgct tactttagcc gcctgttcaa agcaaaaaca ggactatcgc cgagagactt
    36481 tcgtaaattc agctgattca tataatatgt acgtcatttt ttcaaaatat tttgaaatat
    36541 atagataaaa ataaaataac cgcagtttct gcttctatgt aaacaacagc agctactgcg
    36601 gttatttttt agtgatcatt ctcattgttc atcttcaaaa tgttcataat gaaccaactg
    36661 tttctgtgta tcacgtttca aaaattcttt aggcgttatg ccgaactctc tcttgaatgc
    36721 tttcagaaaa tgcggataag aagaaaaacc acttttacta tatacagcac tagagctgcg
    36781 gtattctcgc aacaagtgct tggcatagat cagctttttc ttcattacat actgatgaaa
    36841 cgtaaatcca gtgtactttt taaattctcg tgtgatatgg tatttgctga caaaaaactg
    36901 cttttccatt tcctctaaag aaagcggatg agataaattt tttgagatat attgtaacat
    36961 ttgttccaca cgctcattag gaactacagc attctgttct tgcgcactct cttctttttg
    37021 tacaacttga ttgagaaaaa tcaagtattc taccagagct tgttcatatt ttatgtcttc
    37081 accgtaaatt tttttatcag gaggatggtc gataaatgat aatttttgct tcaatacttc
    37141 ctgttctatt tgcaatacac gactcctctt ctctcccatt ggttgaaaac aagccgatag
    37201 attagaccat ggcgtggaac gactttctag aaaagaaggg gtgacaaaga tatagacacg
    37261 ctcgaataca tctgaagatt ggcggacgat acgatgcaaa tcattggaat ggatgagcag
    37321 cacacttcct gctttgatcg taaattgtcg tccgttcaaa taaaaagtcg cttctccctt
    37381 taatgtagca tggatctcat agaaatcatg atgatgatag atcgttttat tttggctgac
    37441 gacatctttg acatgataga tttcatactg atcagaaacc atcgtattga cttgttcacc
    37501 gctgattttc ttttggagca tgtgccgcac ctccttttgt tcacttatgc acaattttat
    37561 tataccacgt tttttgtaat caaaaagagc tgcttttata tactcttata aaaaagaaga
    37621 gacgtggcat cttctagccc cgccccctcc attctaaata ttagttaacc aacgtcttct
    37681 ttacattatt caagaagact tcttccataa cgaatcccat catcttttgt ttcttcaaga
    37741 accacataca ggtacggacg attcttcatg atgatttctt tgatttttgt gtactcgatc
    37801 acaccgtctc ccaaatttac cggcacgatt ttttcctgtt cgacacgaaa atctttcaaa
    37861 tgaaccgcac atatcttttc accaaaacgt tcaaaagctt cttctaccaa ttggacttgt
    37921 tcttgataat cactacttgt gatcaaattg gtcggatcta aaatgattcc tagatagtcc
    37981 gaatctactg cttggatcag ctgctctacc cttgctaaag aatacaccgg atgattcaaa
    38041 cctggttcta tccccaccat catttggtat ttttctccag cttttaccaa gcgacggata
    38101 acggacacag cttcagcaaa tgcttcatct gtgaaatttt cttccgtata ttggatttcc
    38161 ggcaaaacac atccagtttc cgaagcaacc atcgacgcac caaaggaaga agcatagcga
    38221 agatactgct cgaactttgt cagcaactct tgtcgtatcg ttaaatctgg atgaatcata
    38281 ttgatatagc aacttaaaat tcctacggtc acttcttgct tttctaaaac tcgtttgaca
    38341 taatttccca tcccagaatt gatctcattt gcgccagaag gtatttcagg aaacgaaaga
    38401 cctaaggcga gctgcagtgt atgaatccct tgttcttttg ttttttctgc aagatcttct
    38461 atcgtttgga cttcggaaag atcatgtcct ctaattgtaa tgttcaacat aactattctc
    38521 ctaactcagc atattgggta gccacatgac gatttgcggg aagtaagtaa tcaaaaacaa
    38581 gacgactaag ataaccccat aaaaaggaag aagcggtttc agcattgcct ctgcttttac
    38641 gcctcctaca cttgcaccga catatagtcc tgtacctact ggcggcgtga tggagcctac
    38701 acacagattc atgattgaaa acaacccgta atgtacagga tcaaccccta cacttgtgac
    38761 gattggtaaa aatattggtg taaaaatcaa gatagcaggc gccacatcca taaacatacc
    38821 caccagtaat aacacaagat tgactaataa cagaataata aatttattat ccgttacgcc
    38881 cataatcaac gaactaattg cctgaggaat accggtaaac gccatggcga aagacatcat
    38941 ggaagaagta gccaataaga acatgatcgt ccctgacatt gctactgatt gaaacaacat
    39001 attaggtata tcttttagtt ttaccgtttt atagtaaaac atagcaatca acagggagta
    39061 aatgacagct atcgctgaag cttctactgc tgtaaatatc cccgtcaaga taccaccgat
    39121 aatgatgaca atcaatagaa ggctaggaat cgcctctttt attatttttc cagcatgagc
    39181 catctgtcct ttttcgacaa cagggtattt attcttttta gcaacgatga atgctactag
    39241 catgattcct agcgcccaca aagcgcctac gagataacct cccataaaca aagcagagat
    39301 cgaagcacca ccagcaacaa gtgaatacat gataaaagca gtacttggag gaatgaccat
    39361 acctgtcgga gctgacgcga tattgaccgc tgtcgcaaac ttccggtcat atccttcttc
    39421 cacttgctga gggatcaaca ctccgccaat cgcggtagaa gcggcaatgg ccgagctaga
    39481 gatggaacca aataaagcat tccctaacac attcgtatga gccaaagccc ccggcacgcg
    39541 cccgacgaac aacatcgcaa aatccactag tttcttagct atccctccat tattcatgat
    39601 aatgcccgag agaacgaaaa acggaatagc gatcagcgaa aaactattga tacttcccac
    39661 cattttttga gctgaactaa aagcagaaac atcaaacggt ataactaaca ataaagtcat
    39721 catcgatgag aatgcaatac ttatagcaat cggcatccct aatacaagca acacaacaaa
    39781 tacaaaaaat aacactaatc cggcttctat tgccatttta ttcctccatt tttatatttt
    39841 ttcttgaatt gctggcttgt tgcttaatgt atcgattaga ttgatcaggc tatagataat
    39901 gatgagtacc cccgatacag gtactgacag ataaacatat cccataggaa tacttaaaga
    39961 aggcgagatc tgccccattg tcaatgtgac agcattccat ccgccataca ccatgatcat
    40021 cgcagcaaat accagaaata aactttgaac aacaacttct aaaattttac tttttttgcc
    40081 agttaatttt tcacttaaaa ccgttacggc taaatgtgct ttttgtccca caacatacgc
    40141 ggaagacaac atggcaaacc agacaagacc gaatcgaaca agctcttccg tgaccgtatt
    40201 tggatttcct aaaaccgttc tggaaaatac ttgccataaa acaacagcga ccatcccaac
    40261 aagcaaaaca gctcccagga tctctaatac cttgtttaaa atcattttca tcacagctac
    40321 gctccttgct cttggatctt ttgataaagg tctttgtagg taggatcgtt tttgaatata
    40381 tcatgaagtg gggcaactgc ctctcgaaac ggtgtattat ctacatcatt gaattggact
    40441 ccatgttctt cttgtgctaa agctttttct tcttcaattg cttttgcaaa agcttcaatt
    40501 tcaaaaaccg ttgattcttt cgctgcttct tcaatggctt tttcctgctc atcagttagc
    40561 tgttctttga tggcatcatt catgatgatg atatctggca cacgtgtatg ctcatcatag
    40621 gaatagtatt tagccacacc accatgtcca gctgtataca agacaaattc attgttttct
    40681 gatccgttga tcaaattgga ttgaagagac gtgtacactt catcacttcc cataggtacg
    40741 ggcgaaccgc ctaaaagctc taccattttg atagcggttt cgctttgcat cacacggatc
    40801 ttctttccct tcaaatcttc cggcgtatgg atcggaccgt ctaccatata aaagctgcgc
    40861 tgaccagaat cataataggt caatccggta aatcctaaat ttttggtcga ttggtagatt
    40921 ggctccatta tttcttcatt atccatcacc ttaaagaaat gttcttcatc attgaataga
    40981 taaggaactg aaaaaatcga ataatcttta gagaaacttt ccaacgctga ggcacttact
    41041 tttgtgaaat ctactgctcc tgactgtgtc aattcgatca gttcacgttc tccacccagt
    41101 tgtccatctg ggaaatactg gatctgtacg gaaccatctg ttttttcttt tactaaacgc
    41161 ccaaattcat tcattgcatc aattactggc tggctgttgc ttgcataagc aaagcgcaag
    41221 gtaactatct catttacatc tgcatctgcg acagttgcca cactagaatt atttccgcag
    41281 ccgcttagac ctagcagacc taggctaagc gaacttgctg caaacagcag tgatgttttt
    41341 ttcatatggg tttcctttct tttaacaata agagacgagc aattctgtca aacaaagcac
    41401 cgttaacgac tgtccataag gcattgctgt gattccgata ttccgataaa aatctaaact
    41461 gtcccccatt cctgtcccga ttgaaacgtt ttgaacttct cctttttcat cgatttgttc
    41521 aagcaatcct tggatagcac gataagcgac ttctttatat tcttgtggta aataacgttt
    41581 atggaccgct ttcaaaatac cgtaagcaaa tcctgcagtt gcagaagatt ctacgtaaga
    41641 agaaggatca tccaacaacg tatgccataa accgctttca gactgataag tttttaatgc
    41701 acgcacttgt gcttcaagtg tttcgatcaa taatttgcgc aatgcatctt ctttcgataa
    41761 ttctaagatt tcaatgatct ctggaatagc aatcgtcaac cagcagtttc cacgagccca
    41821 aaatgcttcg gcatagttat gatttccttc aaacgtccag ccgtgatacc acaatcctgt
    41881 tcgtttatcc atcaagtatt tgatatggat cataaactgg taacgagctt cttctaaata
    41941 ttctgggcga ttcaacagtt tcccgatttt tgccaaagga agcactgtca tcatcaatgt
    42001 atcgtcccac aactgattct tattttccgg tccatatgtg gcatgctgta acccgtcttc
    42061 atttgtccga ggcatgtcat tcatgaccca ttctgcccat tgttctaaat aaggcaaata
    42121 gcgtgaatct ttcgtgtctt catacaagta agccatagtc aatagcggtg ccattgtatt
    42181 gacgtttttg ggcggagcgc cttcttccat acgatcctca aaccattcat tgaccacttg
    42241 ataagcgcga ggattttttg tcatctggta atttttataa ataccatata gaccgacacc
    42301 ttgtggccaa ttccacactg tccagctttt gtcatctact ttcagtccat caaagtccaa
    42361 caaaaattca ccattatcgt ctttgatcgt tgtcaggttt tcaacgagca ggtcaatttt
    42421 ttctttcact aattctttag gtacaaaaat ctcttcttta ataatcatct ttacgccacc
    42481 tttttaaacg ctttcatttt atatccttaa tatagcagta ataccgtcca ctataaatat
    42541 cgctttttga acaaaaactg tctaaaattg acctaaatgt taaataacaa aagtaacttt
    42601 cagttacttg gaaattaaaa aaagaacttg gagaaacaac gttttccaag ttctttttca
    42661 tctattttct tatctttttt ctacgctttc ttttgcttat ccgttgtaag catcaatgat
    42721 gatctgtttc aattcagaga tcaatggttc tttcggatta gctgtcgtac attggtcttc
    42781 gtaagctaat tcagccatac ggtctacggt tgtatctagt gtttcttgtg tgactccttg
    42841 agcttttaga ctcatgttga tgccgacaga ttttcctaat tcataaacag ccgttgctaa
    42901 agcttcgact aattcctctg ttgtttcgcc tttcagacct aagaattttg cgatatctgc
    42961 gtaatctgta tctgcacgga aatagtcata tttagggaac atcgcatgtt tttgtggatc
    43021 ttttgcatta tagcggataa tatgcggtaa aagaatcgca tttgtccgtc catgagggat
    43081 cccgtattcc ccgccgattt tatgtgcgac tgagtgacaa atacctagga atgcgttagc
    43141 aaacgccata ccagccattg tagaagcgtt gtgcattttt tcacgtgatt ccatatcagg
    43201 tgtttttact gatttttcta aatagtcaaa gactaacttg attgcttgta ggctcaatcc
    43261 acgtgtataa tctgaagcca tgactgaaac ataggattcg attgcatgtg ttaacacatc
    43321 catcccggta tctgctgtga cagatgctgg aactgacatg acgaattgag gatcgacgat
    43381 tgcgacatct ggcgtcaatg cataatctgc taacggatat ttcacatgtg tttcactgtc
    43441 agtgatgacc gcaaatggcg ttacttcaga accagtccct gatgttgtcg ggatacatac
    43501 gaattgtgtt ttttctggtt tgtcgatctt atatgtccgt ttacggatat ccaagaattt
    43561 ttgttttgca ccaaagaaag atgtgtctgg gtgttcatag aacatccaca ttcccttagc
    43621 tgcgtccatt gcagaaccgc ctccaagagc gattactgta tctggcttga agtctgccat
    43681 cagtttcgtt cctgcgtata cggtatttgt cgatgggttc ggttcaacat ctgagaagac
    43741 ttcgatcttc acatcatttt tacgtcgtgc caatacttca cgaactgtat cgcagtaacc
    43801 gaattggacc ataccagggt cacaaacgat catgacacgt tcaacgtttt ccattttctc
    43861 taagtacaat agagagttct tttcaaagaa gatttttggc ggtaatttga accattgcat
    43921 gttatttctc cgtttcgcta cagttttgac attgattaag tttacagcag agacgttacg
    43981 agaaacagag ttcttaccat aagaaccgca tcccaaagtc aatgacggaa tcatttcgtt
    44041 atagatatca ccgatgccgc cttctgcaga tggtgtattc actaaaatac gacaagcttt
    44101 cattcgaaga ccgaatttga catgcaactc ttcgttttct gaatggataa ctgctgtatg
    44161 tcctaatcct ccaagattca acataccttc acacaaatca aacgcatggt ccgtgttgtt
    44221 agctttgatc atggcaagaa ctggagaaag tttttctctt gatagcggat aatctgggcc
    44281 tacgccttcc aattcagcaa tcagcatttt cgttcctttt ggtacactga ttcctgccaa
    44341 ttttgcgatt tccatcgcag aatgtccaac gattgatgga ttgaccgcat attttccttc
    44401 gttcatcact gcatcttcta atttttgcaa ttcatctggt tttacgatat aaacttggtg
    44461 tgcttggaat tctgctttta ctgctgcata gatttcttta tccacgatca cagcttgttc
    44521 agaagcacaa atcattccat tatcaaatgt tttcgaaacg atcaaatcgt tcactgcacg
    44581 tttaattttt gctgtttttt caatataagc tggtacgtta cctggaccta cacctaaagc
    44641 tggttttcct gtagagtaag cagacttgac cattccagct ccacctgtcg ctaagacgat
    44701 cgcgatacct ggatggttca ttagcataga agtcgcttcg atcgaaggat gttcgatcca
    44761 ttggatacaa ttttcaggag ctccggcagc gatagcagca tcacgtacca ctcgtgctgc
    44821 ttctgcagaa gatttttggg cacttggatg gaaagcaaaa acgattggat ttcttgtttt
    44881 caacgcgatc attgatttga aaatcgttgt ggaagtcgga ttcgttgttg gagttactcc
    44941 gcaaaccaca ccgactggtt cagcaatttc aatcaaccct ttttggacat cttcattgat
    45001 cacaccaact gttttatcat gtttgatatt atgccaaata tattcggacg catacatatt
    45061 tttgattgct ttatcttcgt aaacaccgcg gcctgtttcc tctacagcca tcttcgctaa
    45121 tggcatatgt tgatccaaag ctgccattgc catttcatgc acgatatggt ctactttttc
    45181 ttgatcaaag ctttccattt ctttcaatgc tgcatttgct ttttctgcca aatcatcaat
    45241 cattgcttgt acgtcaattg tcttcgtttt ttctttttct attactttag ccatcattat
    45301 cctcctaatg ggtcatggtt atttgttaaa taattcacat gtatatcata caatatttat
    45361 ttttctatgt aaacactttt ttgtgatttt tttcacagtt atttttaaat ataaaattgc
    45421 tttatttaca ggtgtttgta tagaaaaata tataaagtta tttattatta tttatatgac
    45481 acacccgatt attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa
    45541 taaaaaatga ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt
    45601 atttttcttc cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa
    45661 ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag
    45721 tccctggttt gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt
    45781 ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga
    45841 tcgttacgac tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt
    45901 gtttcttttg cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa
    45961 gcattggtaa tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt
    46021 agcagaaaaa tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga
    46081 agctttttgc gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa
    46141 gcaaattatc atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata
    46201 aattgtggta agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga
    46261 tataggcacg tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga
    46321 aatcatgggc atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc
    46381 catttcgagc gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac
    46441 catcgatcaa tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga
    46501 tcaacggtgt agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata
    46561 acccgccgat tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac
    46621 gaagatcttc aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag
    46681 catgagcttt caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt
    46741 agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca
    46801 atttattttg gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt
    46861 gatttttgaa atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt
    46921 gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc
    46981 cagcttcttc tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa
    47041 taacgccagc acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg
    47101 ttcctactgg catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta
    47161 aacgcgcgcc agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca
    47221 ttcatttccc tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca
    47281 agtgcttttt gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc
    47341 ctttttttat gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat
    47401 ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa
    47461 aacgccaagc aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa
    47521 tccctacatt gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg
    47581 ccaccaccat atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta
    47641 gttcaggagg cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta
    47701 tttcttggac ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag
    47761 atgcatttcg tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga
    47821 tctcgatctt tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct
    47881 attcttattc tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac
    47941 ctaaagtact ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac
    48001 ttttctggtt agatctttca tttatcggct ggcatatctt agcattagca actgctggta
    48061 ttggttatct atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat
    48121 tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc
    48181 gtttatcgag aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac
    48241 attcatgtca cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag
    48301 ttaatcattc ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg
    48361 cgattggtac gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta
    48421 ggccgaatag ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca
    48481 gtaacgccat ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc
    48541 tgctgcgtac attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag
    48601 tttgaccgac atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta
    48661 aataataacc aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca
    48721 ataaacctcg agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt
    48781 gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa
    48841 acagtagttg cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa
    48901 taaagatctc cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat
    48961 aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa
    49021 tcatatctgc ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt
    49081 tacttgcttt caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac
    49141 ccgtacgttg ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga
    49201 cagagagagc tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc
    49261 ctgaaagtgc ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag
    49321 tcaccacatg atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga
    49381 ttgctccttt actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta
    49441 agtctttttc cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac
    49501 caacagcaat cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt
    49561 ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga
    49621 tgtatttttc gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa
    49681 gctcgtttac tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa
    49741 cagcccttac gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt
    49801 gctcgaccgt cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg
    49861 tcattgaatc ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca
    49921 tttcattttg tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg
    49981 tttcaactgc tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt
    50041 atgcaagtgt caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg
    50101 aaatcgaagt catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata
    50161 ctaaagccgc agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga
    50221 cctgtaaagg tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt
    50281 ctgtagccat gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc
    50341 ctttaaatac catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg
    50401 catctttttc aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg
    50461 atgcttcgat caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat
    50521 cacccactac cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg
    50581 ccgtttgttt attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga
    50641 caatcgccaa taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat
    50701 ccccatgttc tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga
    50761 tcgttgtaaa atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct
    50821 catcgaattt attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc
    50881 cagtatctgg atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt
    50941 ccatcctatt cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat
    51001 ccttttttta tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat
    51061 ttaaagtgct tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg
    51121 aggataccat caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg
    51181 ttagaagtac aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg
    51241 cgtatggatg tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt
    51301 gaataacgta aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg
    51361 taacgaaaac aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg
    51421 atggaaagtt acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc
    51481 ttatcgagag ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca
    51541 aaactagcgg cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac
    51601 gatagcgctc ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg
    51661 cactaactaa catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta
    51721 ccactttgaa ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga
    51781 tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag
    51841 ccacgatttt cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca
    51901 aacggtagaa ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga
    51961 atgtacctaa ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct
    52021 catcaagtaa ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt
    52081 ctttcgcata gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag
    52141 gcaatggcat ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt
    52201 caatgatccg tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac
    52261 caaaagagat tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg
    52321 tatctccttg tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt
    52381 ctccatataa acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct
    52441 gtaactcttc aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca
    52501 aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg
    52561 gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc
    52621 ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt
    52681 ttttgttttc ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac
    52741 tagtaataga tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca
    52801 aaatgtgtca aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt
    52861 tgaaaagggt gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat
    52921 gcggatgatc aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc
    52981 catccaagaa attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat
    53041 tggattgatg cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa
    53101 agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc
    53161 ccgtgagatg ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg
    53221 agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc
    53281 gcttgatcca atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt
    53341 gggaaaaacg atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg
    53401 tatcgccatc atgagtgaag gccgtgtgat caatttgata caccagaaaa tattttacgt
    53461 catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag
    53521 ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa
    53581 aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt
    53641 accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga
    53701 ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc
    53761 gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc
    53821 gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc
    53881 tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct
    53941 gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt
    54001 ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc
    54061 attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt
    54121 ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg
    54181 ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg
    54241 attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca
    54301 aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg
    54361 ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt
    54421 gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac
    54481 caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat
    54541 tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa
    54601 gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt
    54661 gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa
    54721 tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag
    54781 ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga
    54841 tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat
    54901 tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg
    54961 agaaaagata cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc
    55021 ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg
    55081 tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt
    55141 atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg
    55201 gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg
    55261 atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac
    55321 catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag
    55381 aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg
    55441 aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc
    55501 actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta
    55561 ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc
    55621 tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag
    55681 gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc
    55741 tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt
    55801 tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca
    55861 agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact
    55921 attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt
    55981 agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga
    56041 tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt
    56101 gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct
    56161 agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc
    56221 atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga
    56281 ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat tccgtatact
    56341 ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc
    56401 cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga
    56461 tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg
    56521 tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca
    56581 tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg
    56641 ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt
    56701 tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg
    56761 cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc
    56821 aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac
    56881 tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt
    56941 tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg
    57001 tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact
    57061 ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat
    57121 ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta
    57181 acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat
    57241 aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag
    57301 gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga
    57361 gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca
    57421 ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta
    57481 aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag
    57541 cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc
    57601 aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt
    57661 gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta
    57721 aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga
    57781 aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca
    57841 ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt caataactga
    57901 taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct
    57961 ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact
    58021 taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga
    58081 tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat
    58141 tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca
    58201 acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg
    58261 aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc
    58321 tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg
    58381 atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt
    58441 aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta
    58501 agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga
    58561 ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata
    58621 ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga
    58681 ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta
    58741 aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca
    58801 agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct
    58861 gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt
    58921 acttacgata tctacataac ggataattgg attttcaatc gagcgataag aagagtggac
    58981 gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt
    59041 ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc
    59101 gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt
    59161 cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt
    59221 ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg
    59281 ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc
    59341 acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac
    59401 gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat
    59461 caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt
    59521 gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa
    59581 tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc
    59641 aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa
    59701 gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca
    59761 atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa
    59821 tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc
    59881 ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga
    59941 aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac
    60001 gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg
    60061 aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa
    60121 aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat
    60181 cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga
    60241 gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc
    60301 cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa
    60361 aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac
    60421 taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa
    60481 taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg
    60541 cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt
    60601 acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac
    60661 cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct
    60721 ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat
    60781 tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt
    60841 aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat
    60901 agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa
    60961 gcccaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg
    61021 cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt
    61081 agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag
    61141 gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc
    61201 ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc
    61261 aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat
    61321 tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat
    61381 catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga
    61441 cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac
    61501 atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg
    61561 attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc
    61621 aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt
    61681 gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc
    61741 gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat
    61801 tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt
    61861 tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg
    61921 gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa
    61981 gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa
    62041 tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa
    62101 tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat
    62161 gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg
    62221 tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt
    62281 tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc
    62341 ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat
    62401 ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg
    62461 atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga
    62521 taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg
    62581 ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc
    62641 gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt
    62701 tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa
    62761 cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt
    62821 gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc
    62881 acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat
    62941 cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa
    63001 atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg
    63061 caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac
    63121 gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt
    63181 cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc
    63241 gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt tattgatact
    63301 tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact
    63361 ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt
    63421 gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt
    63481 gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc
    63541 tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg
    63601 ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc
    63661 tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt
    63721 atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat
    63781 catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag
    63841 caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc
    63901 agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc
    63961 gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa
    64021 ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa
    64081 gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac
    64141 aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg
    64201 atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta
    64261 agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg
    64321 attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa
    64381 aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg
    64441 ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg
    64501 attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca
    64561 aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag
    64621 attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct
    64681 cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa
    64741 gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt
    64801 tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg
    64861 acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata
    64921 ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct
    64981 cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca
    65041 tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct
    65101 ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac
    65161 gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca
    65221 aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac
    65281 gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg
    65341 ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa
    65401 aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat
    65461 tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt
    65521 cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca
    65581 gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga
    65641 tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta
    65701 tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta
    65761 tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc
    65821 aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc
    65881 gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc
    65941 tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac
    66001 aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt
    66061 tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt
    66121 taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag
    66181 ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt
    66241 ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga
    66301 tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata
    66361 gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga
    66421 tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga
    66481 gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt
    66541 ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat
    66601 tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag
    66661 gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg
    66721 tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac
    66781 gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac
    66841 gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct
    66901 ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat
    66961 gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag
    67021 taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag
    67081 ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt
    67141 ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt
    67201 cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg
    67261 tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag
    67321 taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt
    67381 cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt
    67441 tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag
    67501 gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt
    67561 tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta
    67621 tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa attataacca
    67681 ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac
    67741 tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt
    67801 gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt
    67861 ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat
    67921 ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca
    67981 ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag
    68041 aatattcttc taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa
    68101 gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg
    68161 ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt
    68221 ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac
    68281 gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag
    68341 tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat
    68401 gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga
    68461 ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt
    68521 ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga
    68581 tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca
    68641 tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct
    68701 tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg
    68761 aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca
    68821 aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac
    68881 gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa
    68941 tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata
    69001 attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt
    69061 cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg
    69121 tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg
    69181 cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg
    69241 ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt
    69301 agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg
    69361 gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga
    69421 agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct
    69481 ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat
    69541 aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct
    69601 gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc ataatactat
    69661 ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat
    69721 agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt
    69781 ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat
    69841 gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc
    69901 cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat
    69961 tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt
    70021 gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc
    70081 gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg
    70141 taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg
    70201 catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta
    70261 aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt
    70321 cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg
    70381 cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga
    70441 tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt
    70501 gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt
    70561 tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc
    70621 ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca
    70681 tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag
    70741 gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc
    70801 catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa
    70861 attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt
    70921 cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc
    70981 ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa
    71041 gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt
    71101 tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc
    71161 aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc
    71221 cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa
    71281 gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc
    71341 ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt
    71401 attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa
    71461 agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc
    71521 ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc
    71581 ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa
    71641 catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca
    71701 ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata
    71761 cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg
    71821 catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa
    71881 aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt
    71941 aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc
    72001 aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag
    72061 ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga
    72121 cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc
    72181 ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg
    72241 aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt
    72301 gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg
    72361 atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat
    72421 tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt
    72481 gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg
    72541 ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca
    72601 ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc
    72661 gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag
    72721 ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc
    72781 cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact
    72841 agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg
    72901 gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa
    72961 gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa
    73021 gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt
    73081 atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac
    73141 tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg
    73201 ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc
    73261 catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt
    73321 gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt
    73381 aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa
    73441 aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa
    73501 gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa
    73561 gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat
    73621 atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct
    73681 gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg
    73741 gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg
    73801 cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa
    73861 gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat
    73921 cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa
    73981 gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt
    74041 gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg
    74101 aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta
    74161 cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct
    74221 gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca
    74281 aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc
    74341 ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc
    74401 cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt
    74461 tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc
    74521 gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa
    74581 tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc
    74641 gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt
    74701 agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata
    74761 ggattctcct aattttatga catcttcttt tactggagaa ccatctccat caatgaatag
    74821 cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa
    74881 gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt
    74941 catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa
    75001 acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga gtagtatact
    75061 tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta
    75121 gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat
    75181 ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag
    75241 accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg
    75301 cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa
    75361 aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta
    75421 gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga
    75481 acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg
    75541 catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag
    75601 ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg
    75661 tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg
    75721 gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact
    75781 ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag
    75841 caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa
    75901 aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca
    75961 tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca
    76021 tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta
    76081 gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg
    76141 tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa
    76201 cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta
    76261 cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt
    76321 acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc
    76381 tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg
    76441 agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct
    76501 atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat
    76561 ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat
    76621 gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt
    76681 cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata
    76741 atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt
    76801 cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt
    76861 ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa
    76921 cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt
    76981 ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga
    77041 ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa
    77101 tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt
    77161 ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc
    77221 ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg
    77281 ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac
    77341 aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc
    77401 ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg
    77461 catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat
    77521 gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct
    77581 gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc
    77641 atttattaaa actgcctc
//
LOCUS       DAZKOM010000003        76957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000003 DAZKOM010000000
VERSION     DAZKOM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.003"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..331)
                     /locus_tag="ACG8NF_000178"
     CDS             complement(<1..331)
                     /locus_tag="ACG8NF_000178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470696.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(345..602)
                     /locus_tag="ACG8NF_000179"
     CDS             complement(345..602)
                     /locus_tag="ACG8NF_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470697.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(797..1339)
                     /locus_tag="ACG8NF_000180"
     CDS             complement(797..1339)
                     /locus_tag="ACG8NF_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0470698.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1360..2451)
                     /locus_tag="ACG8NF_000181"
     CDS             complement(1360..2451)
                     /locus_tag="ACG8NF_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0470699.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2459..4669)
                     /locus_tag="ACG8NF_000182"
     CDS             complement(2459..4669)
                     /locus_tag="ACG8NF_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0470700.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4685..7213)
                     /locus_tag="ACG8NF_000183"
     CDS             complement(4685..7213)
                     /locus_tag="ACG8NF_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0470701.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7265..7675)
                     /locus_tag="ACG8NF_000184"
     CDS             complement(7265..7675)
                     /locus_tag="ACG8NF_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0470702.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7688..7906)
                     /locus_tag="ACG8NF_000185"
     CDS             complement(7688..7906)
                     /locus_tag="ACG8NF_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0470703.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7903..8895)
                     /locus_tag="ACG8NF_000186"
     CDS             complement(7903..8895)
                     /locus_tag="ACG8NF_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0470704.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8974..9885)
                     /locus_tag="ACG8NF_000187"
     CDS             complement(8974..9885)
                     /locus_tag="ACG8NF_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0470705.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9904..10248)
                     /locus_tag="ACG8NF_000188"
     CDS             complement(9904..10248)
                     /locus_tag="ACG8NF_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470706.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10268..10546)
                     /locus_tag="ACG8NF_000189"
     CDS             complement(10268..10546)
                     /locus_tag="ACG8NF_000189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470707.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10905..11405)
                     /locus_tag="ACG8NF_000190"
     CDS             complement(10905..11405)
                     /locus_tag="ACG8NF_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470708.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11406..11951)
                     /locus_tag="ACG8NF_000191"
     CDS             complement(11406..11951)
                     /locus_tag="ACG8NF_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470709.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11948..13204)
                     /locus_tag="ACG8NF_000192"
     CDS             complement(11948..13204)
                     /locus_tag="ACG8NF_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0470710.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13565..13876)
                     /locus_tag="ACG8NF_000193"
     CDS             complement(13565..13876)
                     /locus_tag="ACG8NF_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470711.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13899..15374)
                     /locus_tag="ACG8NF_000194"
     CDS             complement(13899..15374)
                     /locus_tag="ACG8NF_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0470712.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15823..16275)
                     /locus_tag="ACG8NF_000195"
     CDS             complement(15823..16275)
                     /locus_tag="ACG8NF_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470713.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16277..16642)
                     /locus_tag="ACG8NF_000196"
     CDS             complement(16277..16642)
                     /locus_tag="ACG8NF_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470714.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16741..21024)
                     /locus_tag="ACG8NF_000197"
     CDS             complement(16741..21024)
                     /locus_tag="ACG8NF_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0470715.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21171..21410)
                     /locus_tag="ACG8NF_000198"
     CDS             complement(21171..21410)
                     /locus_tag="ACG8NF_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470716.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21500..21742)
                     /locus_tag="ACG8NF_000199"
                     /pseudo
     CDS             complement(21500..21742)
                     /locus_tag="ACG8NF_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21855..22091)
                     /locus_tag="ACG8NF_000200"
     CDS             complement(21855..22091)
                     /locus_tag="ACG8NF_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470717.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22269..23228
                     /locus_tag="ACG8NF_000201"
     CDS             22269..23228
                     /locus_tag="ACG8NF_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0470718.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23398..24078
                     /locus_tag="ACG8NF_000202"
     CDS             23398..24078
                     /locus_tag="ACG8NF_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0470719.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24168..25202
                     /locus_tag="ACG8NF_000203"
     CDS             24168..25202
                     /locus_tag="ACG8NF_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0470720.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25216..25551
                     /locus_tag="ACG8NF_000204"
     CDS             25216..25551
                     /locus_tag="ACG8NF_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470721.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25535..28015
                     /locus_tag="ACG8NF_000205"
     CDS             25535..28015
                     /locus_tag="ACG8NF_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470722.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28071..29630
                     /locus_tag="ACG8NF_000206"
     CDS             28071..29630
                     /locus_tag="ACG8NF_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0470723.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29627..30991
                     /locus_tag="ACG8NF_000207"
     CDS             29627..30991
                     /locus_tag="ACG8NF_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0470724.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            31162..32232
                     /locus_tag="ACG8NF_000208"
     CDS             31162..32232
                     /locus_tag="ACG8NF_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470725.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            32247..32915
                     /locus_tag="ACG8NF_000209"
     CDS             32247..32915
                     /locus_tag="ACG8NF_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470726.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            32935..33513
                     /locus_tag="ACG8NF_000210"
     CDS             32935..33513
                     /locus_tag="ACG8NF_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0470727.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            33914..34825
                     /locus_tag="ACG8NF_000211"
     CDS             33914..34825
                     /locus_tag="ACG8NF_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0470728.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(35203..36120)
                     /gene="rnhC"
                     /locus_tag="ACG8NF_000212"
     CDS             complement(35203..36120)
                     /gene="rnhC"
                     /locus_tag="ACG8NF_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0470729.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            36322..36759
                     /gene="zapA"
                     /locus_tag="ACG8NF_000213"
     CDS             36322..36759
                     /gene="zapA"
                     /locus_tag="ACG8NF_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0470730.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            36817..37365
                     /locus_tag="ACG8NF_000214"
     CDS             36817..37365
                     /locus_tag="ACG8NF_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0470731.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            37480..39840
                     /locus_tag="ACG8NF_000215"
     CDS             37480..39840
                     /locus_tag="ACG8NF_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0470732.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            39927..40241
                     /gene="trxA"
                     /locus_tag="ACG8NF_000216"
     CDS             39927..40241
                     /gene="trxA"
                     /locus_tag="ACG8NF_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0470733.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            40423..42201
                     /gene="uvrC"
                     /locus_tag="ACG8NF_000217"
     CDS             40423..42201
                     /gene="uvrC"
                     /locus_tag="ACG8NF_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0470734.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            42346..43245
                     /locus_tag="ACG8NF_000218"
     CDS             42346..43245
                     /locus_tag="ACG8NF_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470735.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            43238..44500
                     /locus_tag="ACG8NF_000219"
     CDS             43238..44500
                     /locus_tag="ACG8NF_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470736.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            44661..46559
                     /locus_tag="ACG8NF_000220"
     CDS             44661..46559
                     /locus_tag="ACG8NF_000220"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0470737.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(46889..47608)
                     /locus_tag="ACG8NF_000221"
     CDS             complement(46889..47608)
                     /locus_tag="ACG8NF_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0470738.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    47798..47845
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            47945..48277
                     /locus_tag="ACG8NF_000222"
     CDS             47945..48277
                     /locus_tag="ACG8NF_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470739.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            48264..50267
                     /locus_tag="ACG8NF_000223"
     CDS             48264..50267
                     /locus_tag="ACG8NF_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0470740.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            50286..50756
                     /locus_tag="ACG8NF_000224"
     CDS             50286..50756
                     /locus_tag="ACG8NF_000224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0470741.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            50777..51361
                     /locus_tag="ACG8NF_000225"
     CDS             50777..51361
                     /locus_tag="ACG8NF_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0470742.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            51376..52362
                     /locus_tag="ACG8NF_000226"
     CDS             51376..52362
                     /locus_tag="ACG8NF_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0470743.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            52352..52663
                     /locus_tag="ACG8NF_000227"
     CDS             52352..52663
                     /locus_tag="ACG8NF_000227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0470744.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            52685..54466
                     /locus_tag="ACG8NF_000228"
     CDS             52685..54466
                     /locus_tag="ACG8NF_000228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0470745.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            54459..55835
                     /locus_tag="ACG8NF_000229"
     CDS             54459..55835
                     /locus_tag="ACG8NF_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0470746.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            55842..56477
                     /locus_tag="ACG8NF_000230"
     CDS             55842..56477
                     /locus_tag="ACG8NF_000230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0470747.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            56507..57862
                     /locus_tag="ACG8NF_000231"
     CDS             56507..57862
                     /locus_tag="ACG8NF_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0470748.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(58054..59448)
                     /locus_tag="ACG8NF_000232"
     CDS             complement(58054..59448)
                     /locus_tag="ACG8NF_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0470749.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            59540..60511
                     /locus_tag="ACG8NF_000233"
     CDS             59540..60511
                     /locus_tag="ACG8NF_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0470750.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            60508..61290
                     /locus_tag="ACG8NF_000234"
     CDS             60508..61290
                     /locus_tag="ACG8NF_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0470751.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(61459..61830)
                     /locus_tag="ACG8NF_000235"
     CDS             complement(61459..61830)
                     /locus_tag="ACG8NF_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0470752.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            61964..62407
                     /locus_tag="ACG8NF_000236"
     CDS             61964..62407
                     /locus_tag="ACG8NF_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0470753.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(62450..63259)
                     /gene="yidA"
                     /locus_tag="ACG8NF_000237"
     CDS             complement(62450..63259)
                     /gene="yidA"
                     /locus_tag="ACG8NF_000237"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0470754.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(63278..64645)
                     /locus_tag="ACG8NF_000238"
     CDS             complement(63278..64645)
                     /locus_tag="ACG8NF_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0470755.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(64629..65459)
                     /locus_tag="ACG8NF_000239"
     CDS             complement(64629..65459)
                     /locus_tag="ACG8NF_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0470756.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            65622..66059
                     /locus_tag="ACG8NF_000240"
     CDS             65622..66059
                     /locus_tag="ACG8NF_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0470757.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            66135..66755
                     /gene="rpoE"
                     /locus_tag="ACG8NF_000241"
     CDS             66135..66755
                     /gene="rpoE"
                     /locus_tag="ACG8NF_000241"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0470758.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            66944..67450
                     /locus_tag="ACG8NF_000242"
     CDS             66944..67450
                     /locus_tag="ACG8NF_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0470759.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            67467..68366
                     /locus_tag="ACG8NF_000243"
     CDS             67467..68366
                     /locus_tag="ACG8NF_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0470760.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            68453..69337
                     /locus_tag="ACG8NF_000244"
     CDS             68453..69337
                     /locus_tag="ACG8NF_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0470761.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(69529..69600)
                     /locus_tag="ACG8NF_000245"
     tRNA            complement(69529..69600)
                     /locus_tag="ACG8NF_000245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69565..69567),aa:Arg,seq:ccg)
     gene            69660..70994
                     /gene="rpoN"
                     /locus_tag="ACG8NF_000246"
     CDS             69660..70994
                     /gene="rpoN"
                     /locus_tag="ACG8NF_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0470762.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            71143..72234
                     /locus_tag="ACG8NF_000247"
     CDS             71143..72234
                     /locus_tag="ACG8NF_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0470763.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            72275..73276
                     /gene="gap"
                     /locus_tag="ACG8NF_000248"
     CDS             72275..73276
                     /gene="gap"
                     /locus_tag="ACG8NF_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0470764.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            73386..74576
                     /gene="pgk"
                     /locus_tag="ACG8NF_000249"
     CDS             73386..74576
                     /gene="pgk"
                     /locus_tag="ACG8NF_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0470765.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            74680..75435
                     /gene="tpiA"
                     /locus_tag="ACG8NF_000250"
     CDS             74680..75435
                     /gene="tpiA"
                     /locus_tag="ACG8NF_000250"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0470766.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            75551..76849
                     /gene="eno"
                     /locus_tag="ACG8NF_000251"
     CDS             75551..76849
                     /gene="eno"
                     /locus_tag="ACG8NF_000251"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0470767.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        24693 a        14351 c        14858 g        23055 t
ORIGIN      
        1 atgaattcat tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag
       61 acttgtcata caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca
      121 atccattaaa cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa
      181 tttttatacc ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt
      241 gaatttcttg cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg
      301 attcaacgat ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag
      361 ccatgtagag cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt
      421 actctacatt ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca
      481 atgttcggat tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat
      541 acaaacaatc ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc
      601 atctcgtatc catctcctaa aaaatagaat agttcttata gttattcata agacatattt
      661 tttccgtttt tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca
      721 tagtttttct taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga
      781 gtgttcattt tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct
      841 tttgagcttc ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg
      901 tttcttctcc aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa
      961 gtttcccagt tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac
     1021 aacgatctgt catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac
     1081 tccattttct agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt
     1141 gcgctgtctc gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc
     1201 cacttgtttc ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt
     1261 tttcagaaga aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta
     1321 aaattatccc ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag
     1381 actacgaatt tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc
     1441 ggcattctgt tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc
     1501 ggtgtgacca tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc
     1561 tgcttgtaaa tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt
     1621 ataatcttcc cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc
     1681 taattgagat acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc
     1741 aatgatccga tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt
     1801 ttttgttggt aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata
     1861 ggacgttatg atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc
     1921 cgcatagcct ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca
     1981 ttgattaaag ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa
     2041 gttcggaaac ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg
     2101 tgggtaggga caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga
     2161 ttgtgactgt ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa
     2221 ctgatacttt acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg
     2281 tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc
     2341 tagacctgta gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag
     2401 aaaaaaggga agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc
     2461 atcctttctt cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc
     2521 ctggttttgc gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct
     2581 ctgttggctt atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg
     2641 gtttactttc agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg
     2701 ctaccgacga attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc
     2761 catttttcgc ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta
     2821 attgtggcac gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac
     2881 ctttatttaa caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg
     2941 attgtacctg ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg
     3001 aaagaccaga attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat
     3061 gataacgaac atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa
     3121 agggattaac cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact
     3181 tttgaaatcc atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg
     3241 ctaatcctgc acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt
     3301 gtttcatttg agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg
     3361 cagctcctaa ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata
     3421 ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat
     3481 cgtctactat gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt
     3541 gaaacatcaa gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa
     3601 caaaagaagc gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa
     3661 agataaaacg aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg
     3721 gcaccataga accaacgact gctttataaa cagctttagt tggttgaata tagacacgat
     3781 aagccttctt tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag
     3841 ataattcttc acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat
     3901 acaattcttc taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg
     3961 ctttataata aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa
     4021 caccattgac atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc
     4081 cttctacctc cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta
     4141 cgttactacc atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa
     4201 acaccatttt tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat
     4261 acaagacgac aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa
     4321 aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga
     4381 caaaatcaag gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt
     4441 ttagaaaatc tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag
     4501 ataaataaga agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca
     4561 cctgcttttc tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta
     4621 aaaaaaacaa aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc
     4681 atttctattg catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg
     4741 ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac
     4801 gtccataggg atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt
     4861 tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat
     4921 caaaagcaaa gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa
     4981 attgtgtatc taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa
     5041 taatactacg tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg
     5101 ttttcttact actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca
     5161 aacacaaaga ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta
     5221 atttcaaatc ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt
     5281 ctccataaga aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac
     5341 catattctcg aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc
     5401 caacttgttg tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc
     5461 cttttaaaag gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt
     5521 gtttcgcatc tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat
     5581 ttgtagcatc taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat
     5641 gtttctgata atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg
     5701 gatccacaaa taaactttgt acattcataa acgaactata caaaaagagc aagccaacta
     5761 aaaaggtctt ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg
     5821 cacctttgat tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat
     5881 aaattaattc ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt
     5941 gccgaccaat ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt
     6001 cgccaactgc ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc
     6061 ctaataaaca tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa
     6121 tcgtatgaag ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac
     6181 cgtatacaat aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct
     6241 ttaaatgttt tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt
     6301 gaaccaattc cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa
     6361 tacctccctg tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag
     6421 aaagatcctg agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa
     6481 taggcaacaa tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat
     6541 gttccttcgg tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt
     6601 gttcttctac tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct
     6661 ctttttttac taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat
     6721 aaggatcact ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg
     6781 ctaaacttcc tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg
     6841 ttgctttttc tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc
     6901 gttccaacaa tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag
     6961 aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca
     7021 ccaactccac atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta
     7081 tttcctgtgt cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg
     7141 cccagataca atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt
     7201 ttacaagcac caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct
     7261 ttttctaatc ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt
     7321 ttgatacctt gtcccagtta taatacacac aatcaccatt catcatacga atcatataac
     7381 aaaaccaatc ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg
     7441 ctaacacaca taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag
     7501 aaatgaccca accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa
     7561 aagttgctgc attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt
     7621 gtacgcgata aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac
     7681 tatcctccta atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg
     7741 ttggatcatt taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc
     7801 ctacttttga tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat
     7861 agccaccttg cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc
     7921 tttcctatcc aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt
     7981 aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac
     8041 cgttgctttt acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac
     8101 ttgatcaact ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc
     8161 ttttgcattg tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt
     8221 cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt
     8281 ttctgtattc tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt
     8341 tgaaatagaa aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact
     8401 ctctggattc atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc
     8461 aatttctttt aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc
     8521 tggttcttga ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg
     8581 ttcttgttca tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga
     8641 ttcaatttca ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg
     8701 ttggatttca tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc
     8761 caagccccct gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt
     8821 tgaatggatt ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag
     8881 ttgtcgtttt ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca
     8941 acgataagct tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt
     9001 tctctttctt ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga
     9061 aagtgcaaca tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt
     9121 tttgatttca atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa
     9181 attcccttca ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc
     9241 acctgctact tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact
     9301 agtgacaatc ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt
     9361 taccgatact gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc
     9421 tgatttttca acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt
     9481 tggctgttcc gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg
     9541 ttccggagtt gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc
     9601 gatcgaatca gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt
     9661 ttcaggtttc tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt
     9721 tagctctgtc tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt
     9781 aggtgtttct acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa
     9841 tgaaattcct agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat
     9901 tttttatgca attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa
     9961 gaactcccgt ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa
    10021 tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc
    10081 atccgaaaat tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa
    10141 aggatctcta taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac
    10201 atagaacaat gccataccac ataccaccaa aaataataat cgccacataa aaattccccc
    10261 ttttcttcta cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat
    10321 ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt
    10381 cttcatagcc atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct
    10441 cagcttgcat gacataacca aactctgcac catatcgttc tactgttaat aactgttgac
    10501 tacgcaataa tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac
    10561 gtttgtgaaa taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc
    10621 gataatctgg cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta
    10681 ttcgttcagc ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt
    10741 ttttatcacc tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg
    10801 ctctcctaaa aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag
    10861 aaaccgattt ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc
    10921 aaatgacatt ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg
    10981 gatagaatca cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac
    11041 ttacgcttct tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt
    11101 tctactaagc gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca
    11161 tgtttttcaa tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct
    11221 ggatacctta gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac
    11281 gattctaatc gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct
    11341 ggattttcta atactgttgg atcacgtgac caatacgcac caacataaat cataattttt
    11401 ttcatttaga caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg
    11461 gcatagcgac caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt
    11521 tcttctgttg gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata
    11581 agaggttttt cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag
    11641 ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat
    11701 tctagccttc ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta
    11761 cgaacttcta ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct
    11821 tttaccgctt gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt
    11881 tcatttaatg cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta
    11941 tatgcattca tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct
    12001 cgtcatttag ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat
    12061 ctgcttcttg taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac
    12121 gatctaatga gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata
    12181 attttgccca tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa
    12241 gttttgcatt aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa
    12301 taaatgcttc tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat
    12361 aaataccata tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa
    12421 aacgtttctc ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata
    12481 atccttcact tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct
    12541 taaagtccat ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat
    12601 gttcttccgt ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca
    12661 cacgtgtata accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc
    12721 gccaatctgt ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa
    12781 attgcacaag tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc
    12841 cacgaacaga aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca
    12901 gatccattcg taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac
    12961 gcaaataatc aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt
    13021 ccttgattcg ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa
    13081 tgtaatcttt tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat
    13141 ctccacttat attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg
    13201 tcattttctc gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca
    13261 ccgatatatc aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag
    13321 ataatcactt gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc
    13381 cccccatatt atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc
    13441 tttgtgagct gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta
    13501 atgcgcaccg ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata
    13561 ataactacaa aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa
    13621 cgcgataaat tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag
    13681 tcaatcgttc tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa
    13741 cacgttcttt gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat
    13801 aaaacgttct ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat
    13861 aacaactctc tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa
    13921 aaacttgttc cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac
    13981 aactgattat catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag
    14041 acaatcattt cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat
    14101 ggcgcaaaaa actctacagg tacatcaaat cctggaaaag catatccacg accaggttga
    14161 tctttgttgt aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg
    14221 ggagacaatc gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa
    14281 tattctgcat ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt
    14341 aatactaatg gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca
    14401 taaaaagctg cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa
    14461 tttttccctg gttcatattt aggatctttc atggttttca tataggcata ccgttgattc
    14521 atttctttta atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca
    14581 tgtcctttga atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa
    14641 atatctactg tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc
    14701 ccaccaccga ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa
    14761 gttttcatta aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca
    14821 gaatctgaaa ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca
    14881 cgcaaaaata cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca
    14941 ccatctaaag gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt
    15001 aatctcattt tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga
    15061 ttgtcaatta acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct
    15121 tttagtcgcg accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac
    15181 acaatactta ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga
    15241 taaaatactt tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca
    15301 taatagcaaa accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa
    15361 aaatgagctt tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac
    15421 ttttgacaaa aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa
    15481 agatttaatc gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc
    15541 caaatcactt cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca
    15601 cgtaaaaaca tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc
    15661 gttggattgt ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct
    15721 ctcaaagtat ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat
    15781 aaaaagaata ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc
    15841 cttagaatca acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt
    15901 aggattattt gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga
    15961 atattcacga ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa
    16021 tttcacagga tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg
    16081 tcgtgcaatc tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat
    16141 ttctcccgta taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc
    16201 aaagcgtacg gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg
    16261 aaattttaat cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa
    16321 catcttgata gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat
    16381 tcactcgttc tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt
    16441 ctggtaatcg taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt
    16501 cttctcgcga acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct
    16561 ttggtggatt aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca
    16621 tacgcaatgc tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta
    16681 aatatttcta cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga
    16741 ctaatctttg gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac
    16801 taatccaaga accataagta tactacttgt ttgttcacct gtttttggca acattggtac
    16861 aacagtagct ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc
    16921 cacaatttga accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc
    16981 aaacgtttct ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt
    17041 tcctgcaaga ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc
    17101 ggccgcaaag actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta
    17161 gccttgaaca attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc
    17221 aagcacagtt tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt
    17281 cgtcgtaatg gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg
    17341 agaagccaat aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg
    17401 caacgcacgt gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac
    17461 aaacgttaag gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt
    17521 gtccattaga acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac
    17581 agcgtctgta atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg
    17641 tgtagccgtt gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat
    17701 gtctttatgg tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc
    17761 acgaccattt atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc
    17821 tttggcaata aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc
    17881 ggttgcttta tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt
    17941 aatttcaacg gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc
    18001 ttcaaagaca gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc
    18061 gttgatatcc gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac
    18121 gatcgtttga cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt
    18181 gactttgtct gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg
    18241 cgcattcgtt gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa
    18301 gttggtataa gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc
    18361 accatcggct ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac
    18421 agaaaccact aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc
    18481 ttgtttatct gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc
    18541 atcggcaact agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat
    18601 tgcttgattt ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt
    18661 gttccattct tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg
    18721 gatcactttg atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa
    18781 aacgtaattt acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag
    18841 tgtaccgtta gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt
    18901 atcatttgga atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc
    18961 tgtattagca agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg
    19021 ttgttcaatt actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt
    19081 gacttcaagt ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta
    19141 cgtatcaaag acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac
    19201 ggttttcacc actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt
    19261 tggtttgtta tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc
    19321 aatggttcct ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac
    19381 aacaatttca attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa
    19441 aacatatttg ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt
    19501 caaggtaatt tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc
    19561 taaagaagct gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat
    19621 gtttccttgt tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa
    19681 cacaccaatt gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat
    19741 tttattcaat tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc
    19801 attgccgtga atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa
    19861 aaaatcattt ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt
    19921 attaaaagtt gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg
    19981 atcataatca acacggatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat
    20041 aaattgtgcc gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata
    20101 agagatcaag cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt
    20161 agctgtataa acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg
    20221 tccattaatc tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga
    20281 agtaacacca atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt
    20341 tgcacggcga acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc
    20401 tgtttttgga atgttctgct cttttttctt ttgattttca atactagccg tagattcttt
    20461 gtttggtttc gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt
    20521 ttcctcattt tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat
    20581 tcgtttcttt tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc
    20641 ggcagctttt tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt
    20701 ttcttcaact ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt
    20761 ctgatcgaga gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac
    20821 agtggcttca ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc
    20881 gacttgggca atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg
    20941 agcagttccc accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca
    21001 attcattttc gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata
    21061 aaacaaaacg ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg
    21121 gcgctcttat ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca
    21181 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt
    21241 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    21301 tctttccaac catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca
    21361 attgttacag gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga
    21421 tccgattcct cagtattatt gtcattacta gctactggtt tggttgttct actggcttat
    21481 tttgatacaa caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    21541 accactccgc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    21601 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    21661 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    21721 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    21781 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    21841 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    21901 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    21961 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22021 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22081 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22141 ggaatcacaa cttattcgct tcttctttat aaatcgccga ttgtaaaatt aagctagaca
    22201 actaaaaaaa gtcatttgtg ctacactcaa aatagttccg acgaaagaat tataaggagt
    22261 gagtacaaat gacctataac catcttacac caactgaact cgtaatgata gaagcttatt
    22321 tcaatcaatc acaacctgtt tcaaaagtag ccaatctgtt acaacgttct cgacaaacta
    22381 tttataaggt ctatcgtttt cttaaatctg gtggaagcgc aatgagctac tataaacaat
    22441 ataagaaaaa caaacgtaac tgcggaagac atccgattgt tttacctgat gagcaacagg
    22501 aatacattca aaaaaaggtt gttcaagggt gggctcctga tgtccttatt ggtcgagcag
    22561 agtttcctat tgcttgttcg gtacgcacac tgtatcgaat gtttaaagat aaaacctttg
    22621 acactcacga tttaccaatg aagggaaaaa gaaaaccaaa cggtcataaa gaaaaaagag
    22681 gaaaacaagg gtttcgccgg tcaatccgag acagaaagac agactatacc cagtttgatc
    22741 atgaatttgg tcacctggaa ggtgacacta tcgtgggtgg aaaacataaa agcgcggtca
    22801 tcacattagt tgaacggcta tctaaagtca tcattacctt gaaacccgaa ggcagacagg
    22861 ctaaagacat tgaaaatagg ctgaatcaat ggtttcgttg tgtcccaaaa aacttattta
    22921 aatccatcac atttgattgt ggaaaagaat tctcaaattg gaaaaagatt agtaatacca
    22981 acgatatttc aatttatttt gcggatccag gaacaccttc tcaacgagga ttgaatgaac
    23041 attcaaatgg cttattacgt aaagatggtt tgtataaagc tatggatttt aatggtgtat
    23101 ctgaaacctt tatccaatcg gtggcatcga aacgaaatca tattcctaga aaatcattac
    23161 actatcggac accaatggaa gtatttttga gttacgtcac aaaagatgat ttgtctaact
    23221 taatttgaca aacgaaactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    23281 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    23341 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    23401 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    23461 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    23521 aataccgtgg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    23581 gaaaatccag caactggcca gacaggtcct ttaacactgg atgtagttcc cgaattgcca
    23641 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    23701 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    23761 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    23821 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    23881 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    23941 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    24001 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    24061 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    24121 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    24181 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    24241 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    24301 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    24361 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    24421 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    24481 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    24541 cacatgcaga aacaactgta cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    24601 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    24661 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    24721 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    24781 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    24841 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgcatttt
    24901 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    24961 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    25021 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    25081 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    25141 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    25201 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    25261 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    25321 tatgatctaa gtccaattcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    25381 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    25441 caaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    25501 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    25561 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    25621 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    25681 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    25741 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    25801 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    25861 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    25921 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    25981 tgccggtgcc cccatcaagg ttactagcgg aagtttatta tcagcaggaa gccatacctt
    26041 gcgttttggg atggggtcgt gggatacaaa tactctattg acagatatta ttgctattat
    26101 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    26161 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    26221 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    26281 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    26341 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    26401 agagataaat acgtggaata gttacatctc accaagtgat tcaacaggaa aatataatac
    26461 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    26521 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    26581 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    26641 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    26701 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    26761 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    26821 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    26881 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    26941 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    27001 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    27061 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    27121 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    27181 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    27241 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    27301 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    27361 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    27421 cattcatctt acttacaact atcaattaaa tactgtagat acttctttac aatccgtcag
    27481 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    27541 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    27601 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    27661 aagttattat gaatcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    27721 cacgtctcct tggcaactga caggtacatt gacttcttta tttaagaatg atcaaggtca
    27781 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaagcaat tgattcagca
    27841 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    27901 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    27961 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    28021 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    28081 aacttctgct gttaaaatca gaaatgcttg aaggagtgga cttggaactt ttcagtttgc
    28141 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    28201 aggtttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    28261 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    28321 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    28381 gttacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    28441 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    28501 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    28561 ccggtccaga aagtcaaatt agttattttt atttccaatt attttggttt ggacgtccct
    28621 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    28681 aaaaagagtt tagttttgct attacagaag aaggacgatt gaaatttgaa atatggcttt
    28741 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    28801 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    28861 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    28921 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    28981 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    29041 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    29101 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    29161 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    29221 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    29281 ttgaattatt tgctcgctat gcatctattt tacggacgct gatcaagcag ttagaattac
    29341 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    29401 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    29461 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    29521 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    29581 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    29641 cattggtgct tcccatgaag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    29701 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    29761 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    29821 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    29881 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    29941 taaattaatc gtagcgacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    30001 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    30061 agcaaaagaa gtagtgatct tcggtgctgg ttacattgga ttggaattag cagatgcttt
    30121 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    30181 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaataaattt
    30241 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    30301 acttctgtct caaacaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    30361 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    30421 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    30481 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    30541 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    30601 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    30661 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    30721 cgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    30781 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    30841 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    30901 atacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    30961 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    31021 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    31081 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    31141 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    31201 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    31261 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    31321 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    31381 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccaaacacc tggagtcgtg
    31441 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    31501 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    31561 gtaggcgata ttagtttgaa agaagaatat ggtctagcca taggagatac agttaagtta
    31621 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    31681 tcaccagttc tctatacaac catcaatgct taccaagaaa tccgcttcga aaaagaagat
    31741 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    31801 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    31861 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    31921 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    31981 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    32041 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    32101 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgttagta
    32161 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    32221 gcaattggat aagaaggagg aagaacatga aagcaataga atttaaagaa gtagaaaaaa
    32281 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    32341 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    32401 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    32461 gtaatcaaaa agaaagcgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    32521 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    32581 ttaaacatga acatcgtcaa aaagaagcaa acgaattatt tgagcagctt ggcgtggata
    32641 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    32701 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    32761 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    32821 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    32881 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    32941 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    33001 tccaagggat atctcggcac ttcaacaaga gaaatcgcac aaaaagttgg gatcacccag
    33061 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    33121 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    33181 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    33241 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    33301 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    33361 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    33421 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    33481 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaatagtta
    33541 ggtgaagaag caagatatga cgaagatcgt tatatcttgc ttctttatat aggtagtttt
    33601 tcataaatac aaatttgtta ttctctcatg attatcaggg tatattttta tttattatgt
    33661 cggtgttcca ggaagtgttt taatttaata acgaagaaat taattgtttg tagaatgaag
    33721 gaaactattt attgggtaga tgaatcacca cgaatagtca aaaggagatt tcaggatatg
    33781 ccaaaaatga aaaaaatcat tttagcagta atgttatttt ctacggttat atcttttaca
    33841 gccccttttt tgcttactta agctgatttt aagttttggc ccttagtctg gatctacgag
    33901 gtgagagtca agaatgaaaa aaataattgg agcttttgta tttgcaatca ttatcattat
    33961 tatctgtttt caaatagatg tttttggtca aaaaaagata gttggacaaa cggtcagcag
    34021 tacgaacagt acggttacaa agacaaagga atcatttgat gatacagaac aagagacatt
    34081 aatcaaaatc ggaaaaaatg accgtacact atatacagat gactcgctaa ctaccccact
    34141 tgcaactata aacggcggag agctggcgga gtttctttct gaaacaaaaa atgcttatca
    34201 gatcaagaca aatgatggct atactggata tttagccctc gaagatggaa ccaaagtcac
    34261 aaagaatata caaacaaaac cggaagagct atctgatgca gtgattgtat tagatcctgg
    34321 gcatggcgga aatgatactg gggcattaag caatgatgaa caaacagaag aaaaagatat
    34381 aacattaagt acagccataa aagtaaaaaa ggcgctagag gatgctggag caacggttta
    34441 tttgacccat actacagatg aattagtaca gcttggagat atatgcgact acagtgaaga
    34501 aaaaaaggca gatgtcttta tcagtttaca cgcagactct acggaatatg cgaatgaagc
    34561 aactggaatc acgacatatt attactatgg acaagaggaa acattagcac agacaattgc
    34621 tgactccttc actgatctgc ctctggattc aagaggaatc tccactggga actatcaagt
    34681 tcttcgagaa aatcttcaac cttctatttt aatcgagatg ggttatatga ataatgatag
    34741 tgatttaaag gaattagtaa cagacagtta tcagcagcag attgctgcaa gtttgacaca
    34801 agcattgacc acttattttc agtaataaat aagcccagcc aaacaaaaat agctcctcat
    34861 atttttcgtt tggccgggct tattatcgca gatgcaagct tttaaacacc gtttaaggaa
    34921 gaggtcgtga gcctgccgtt caactctgag tcacaaggag caatttcaaa aaacagcttc
    34981 tcatgtttga tgactattgt gacttgtgac cgaggagttg gcatgagaac accgtttaag
    35041 aaaaaggttg tgaaatcaac gttttgttct gagtattaga gaaacaaacg gaaaaatagt
    35101 tcctcatatt ttgccgtttg ttgagctaat accgaggatc agatgattga acaccgttta
    35161 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagtagctt
    35221 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    35281 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgacggaag
    35341 ggtgattcca gcttcagctg atgctttgtc taactcttcc aaaaagctgg cacgactgat
    35401 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    35461 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    35521 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    35581 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    35641 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    35701 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    35761 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac cgaaatagct
    35821 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    35881 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    35941 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    36001 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    36061 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    36121 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    36181 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    36241 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    36301 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc ggtgattgcc
    36361 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    36421 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    36481 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaata
    36541 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    36601 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    36661 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    36721 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    36781 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    36841 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    36901 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    36961 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    37021 gatcaggtat ttgcctttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    37081 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    37141 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    37201 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc
    37261 caaaatcaat tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt
    37321 accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg
    37381 aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg
    37441 gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa
    37501 acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga
    37561 aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt
    37621 caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa
    37681 attgaaaata tccgtcccca tatgaaacgg attgagatcg gagcagactt gaatggagtg
    37741 gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat
    37801 gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc
    37861 acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact
    37921 gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct
    37981 gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg
    38041 atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta
    38101 tttggcggtg tagtccacga tcaaagtgct tcaggtcaga ctttatttat tgaaccaaaa
    38161 caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt
    38221 caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac
    38281 aatacttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa
    38341 gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga
    38401 catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat
    38461 caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta
    38521 ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa
    38581 atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt
    38641 ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa
    38701 aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct
    38761 ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg
    38821 cattatcctg aattgaaagt ttatggatat aatcgggcaa atacgattaa tgccagcatg
    38881 gagtttgatg tggatacatt aagtccaacg tatcgtttgc tgattggcgt tcctggacga
    38941 agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg
    39001 aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat
    39061 cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa
    39121 gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa
    39181 atggaaaaag caaagaaaaa agcaaatgaa gtcgtgtcag aggcggaaga aaaagcagaa
    39241 aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag
    39301 catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag
    39361 aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg
    39421 ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa
    39481 gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct
    39541 caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg
    39601 ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa
    39661 tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga
    39721 acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc
    39781 tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa
    39841 agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta tactagtcat
    39901 agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg
    39961 aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc
    40021 gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa
    40081 ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca
    40141 ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt
    40201 caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa
    40261 gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta
    40321 ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga
    40381 catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa
    40441 aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga
    40501 acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact
    40561 ggtagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc
    40621 gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat
    40681 cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat
    40741 gaaaaatatc cccgtttggt gattactcgt aaggtactaa aagacaaagc actgtatttt
    40801 ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt
    40861 cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga
    40921 caatgcctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat
    40981 gaagtgaaaa actttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa
    41041 atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc
    41101 cgtgccattg agacaatcat gacttgccaa aaaatgacaa atactgattt actggatcga
    41161 gatgtatttg gttatgcgat tgataaagga tggatgtgtg tccaagtgtt tttcgtaaga
    41221 caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa
    41281 gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa
    41341 gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg
    41401 ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgagtaa gaatgcctca
    41461 gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca
    41521 gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat
    41581 aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa
    41641 ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat
    41701 gcatcaatga gagaagtcat ctatcgcaga tattcgcgag tgattcgcga agggttgcct
    41761 ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc
    41821 ttagaaaatc aattaggaat cgatattcct attgctggca tggtaaaaaa cgataaacat
    41881 aaaacaaatg agttgctatt cggtacagat ttggctgtag taccgcttga gcgaaattca
    41941 ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc
    42001 catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta
    42061 ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca
    42121 gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt
    42181 gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca
    42241 ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc
    42301 tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag
    42361 gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc
    42421 gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt
    42481 ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt
    42541 ggggagaaag attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa
    42601 gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag
    42661 attgaaccaa aaatcgactt ttggatggaa aaatttcagg taaaagggaa aaagacggat
    42721 aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc
    42781 catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct
    42841 gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct
    42901 agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga
    42961 aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta
    43021 ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg
    43081 atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa
    43141 atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact
    43201 ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac
    43261 caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc
    43321 ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt
    43381 caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa
    43441 aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc
    43501 ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat
    43561 gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac
    43621 tggcttgcaa tcgatgcttc gagcgagtca attaggcatt tcaccagaag aagtagctag
    43681 cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat
    43741 gacagtcgga gaagataact cgacagtcca atacgtcgta agttatgtag caacgattgt
    43801 cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa
    43861 aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg
    43921 aaaattaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt
    43981 tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat
    44041 ctcattggat aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg
    44101 cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga
    44161 tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggataca tgcttggatt
    44221 gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt
    44281 cttatcttca tatattatgc cgattcgctt agcaaatgag acagtcggta tgggtggagc
    44341 attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa
    44401 catgtacaaa tcgaatgtac tcgtttatag cgaaggcgga ttatggagtt cattgaaaca
    44461 atcgatttca attatgagga atgaacgaaa aaaagcataa aacaatccta aaatacagaa
    44521 ggaagctgga catctattgt tcccagcttc tttttgtata gacctatttt atttatttta
    44581 ctatattgga tatatattca ttgaaaatag tgtataacaa ctttataata ttgagtatac
    44641 caatttttgg aggcgtttta atggaccgtt atttagattt actttcagaa aaattcccct
    44701 caacggagga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    44761 gaacagagtt atttttgagt gatatacatg gcgaatttcc tgcttttgat catattctgc
    44821 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttttctgaa caaatgacgg
    44881 aaacggatat cagccaattc actattttta cggcttatcc agaatatgca ttaacgactg
    44941 attggtacaa agaacaagat aaatcgcagc tgatccatca tttgatcgat cttttacggt
    45001 ttacgacagt caaatatacc cggtcaaaag ttcgaaaagg attgccgaaa gaatatagct
    45061 atatcattga ggaactcttg tatatcgatg accgtatcaa tggaaaaaaa gactatgtaa
    45121 aaaaaatcat tgaacaactt gttttgatga aggaagaaga aaaattttta acgaaacttg
    45181 cacagacgat ccaaaaatct gtcatcgatc atttgcatat cgttggagat atttttgacc
    45241 gaggaactca agcagccaaa gtgatggatc gaatcatgga tttttcttct gtagatatcc
    45301 aatggggaaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttac
    45361 tgacactttt acggattgca gcacgctata attatttatt tgaattagag aaagaatatg
    45421 ggttgaattt acgtccattg ttttcttttg ctcatcaaac ttatcagaag aatccagtat
    45481 ttgcgcctaa aaacagtaaa aatctatccg aaagagagca agaagaatta gaaaaaatcc
    45541 atcaggcact ttctatcctt caattcaaat cagaacatca gttattggat cgtcgacctg
    45601 aatttaaaat ggacgatcgt aaattgctgg aaaaaatcga ctatgaagca tcaacgattg
    45661 atttagaagg tcagctctat gagttgaaag gaacttgttt tcaaacgatc cagccagatg
    45721 atccaaaaaa attcttggaa gaggaacaaa aagtgattga tagtttgatg tattcattcc
    45781 agcactcttt gagaatgcaa aaacatatga cgtttttgat cgaaaaaggg ggaatgtatc
    45841 tcacgaacaa tcagcatgtg ctgtttcatg gctgtatacc tgtagacgag aagggacagc
    45901 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctgggc ttttttgaaa
    45961 aacagataac tgaaagcgca aaagatctga ctgcaaaaga agatttagca accgatttga
    46021 tttggtatgc gtggagtggg ccattttcgc ctttattcgg caagagtaaa atggcgactt
    46081 ttgaacgtta ttttctcaca gagcctcata cgcacgtcga gaaaagcaat ccttattatc
    46141 acttgcgaga tgaagagtgg ttcagtgaaa aaattttagc agaattcgac acaaaagaag
    46201 aagggtcagc aatcattaat gggcacacgc cagtaaaagt aaaaaaaggg gagtcaccga
    46261 tcaaagcaaa tggaaaagta tttgtcattg acggaggttt atccaaagct tatcaaaaaa
    46321 cgacaggaat tgccggttat tcactattat gcaattctta tggtttccaa atcgtgacac
    46381 attatccgtt tttgcctatt gaacaacaat ttgcaaaaaa atccgaccaa acaaatgtga
    46441 aaaaagtaat cgaacagcaa ctaccacgca aactcaatcg tgatacaaca aaaggtatgg
    46501 aactgcagca gcagattacc cagctcaaaa ggctgttgga ctatcgaaag gacgattgag
    46561 aagaattcca gtgaacattg ttccatagac tggagactga gacaaaagtc gtgtcgcagt
    46621 ctctttttcc ggataaacgg tgttcttctg ccaactcctc ggtcacaagt cacactattc
    46681 aacaaacatg aaaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg
    46741 ctcacgacct cttggtataa acggtgttca aaagccagtt tctcggtatt aagccaaatt
    46801 tatgcaaaaa tgtgaaaagc cattttcaca aaatttttct taatactcga aactaaacag
    46861 ctttttcaca accccctcag aaagtctatt aaaattcatt cacaaagaaa tcgaataagc
    46921 gctgttcaga tggatgtaca cggtgggtaa gttccggatg ccattgaaca ccgaggattt
    46981 tatgcatatc atctttgctt tgaaccgctt caacgagtcc atcgttagaa aaagcgatgg
    47041 cttttaataa aggtgaaaga tctttgactg cctgatgatg gtaagaattc acaaaatatg
    47101 gatcggggag aaggttgctt aacaaactgt ctgaaacaat cttgacttcg tgagtcgtaa
    47161 attgcggggc agtgggctgt tgttcatgtt ttactgacca ctttggatat aatgaaagat
    47221 cttgataaag tgtaccttcc aaaacgacat tcaatagttg gagtccccgg cagactgcaa
    47281 aaattggttt tctttgtttc aatgcttcca agatcagtgc ctgttcaaat tggtctcgtt
    47341 gaatatttgt ttccgttagc tttggatgag gatcttccat gtaaagttgt ggactgacat
    47401 cttgaccacc tgcaagaata agtttgtcga tttgtccaat cagttgagga gctgaatctg
    47461 gagacatgac tggcagaata agaggcaatc cttgagcatc taaaactgca tccacgaatc
    47521 cttgcggtgt atagctcact tgatttcctt ggaaagtatc tgttgctcga atcagttgat
    47581 ttccggcaat gccgatgatt ggtttcattt gtattccccc taaagtagac ttattgtaac
    47641 aaggatttaa aaaaaattaa actatgtaag acttgcccct atacttatct gttcttgctg
    47701 tgttactatc tgtaaagaga aatttcgtgt gaaattcatg acgatagttg aaaatattgc
    47761 ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat
    47821 ctagttcttc aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat
    47881 ctttagaggt acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa
    47941 ggaagtgaag caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga
    48001 aaagcgtgct caaacactgc ggcagcagct gaagatctat gcaacagaaa aagagcagga
    48061 attggaaacg atccaaacaa actatcgaca acaactaaaa cagttggtgg aagaaaaaga
    48121 acaatttgaa atacaagaat tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa
    48181 ggtgcaagca actttgaaag atcaatatca aaagcatcag agtcaagcga tcggtgcgat
    48241 cattgaaagg gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg accctcattt
    48301 cagataaaag aaatcaggag aggattttac aagcagtaca aggaatccaa aacgttgaaa
    48361 tccgggatct gtatcaaaat agaacaaaca accagtgggt ggagaagtat tttccagata
    48421 ccacgattat tgataaagaa gcaaaattaa gcgctttagg taatcggttg aaagacatcc
    48481 aagaagctat ccaattcata caacaccacg gcgatagccg tcaaaagaaa ctccatttga
    48541 aacgtcaaga actttcctta caagatttgg aagccaatta ttccgaagaa aatttcataa
    48601 ataaattgaa agaagtcctt gatttaaaag aaaagtggga acaactaact gaagaacgtg
    48661 atcgtttaga agaagaggaa gaatggcttc ttcattggca acagctggat attgctccaa
    48721 gtatgtatgc aagtaagacg accatttttg agatggggac tgtgagtgca gcaaattttg
    48781 aaagtttcaa ggaagaactg agtaagcttg atgacgttta tatggaagaa atagattata
    48841 cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa atcagttgct gttatagaag
    48901 aaattgctgg gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag
    48961 aaaaactgca agagaccaag cagcaattat ccattgttca cgagcagcaa aagaagataa
    49021 cgacagcgtt aggtcagtgc agtggctata tccaagaatt tgaatgggta gaagaagtca
    49081 cacttgccgt tgcagaacga gaaaaaataa aagaacggtt tatccatgcg acttatctga
    49141 tcgttttgca aggttgggta gatgcagaag aaaaacagaa tttgctgcat gtgttaaacg
    49201 aaaccgtatc agaagaagat atatacgtca gtttcgaaac accaaactca gaagagattc
    49261 agcaggaagt ccctactaaa ttgaaaaacc atccgcttgt cgaacctttt gaattgctga
    49321 ctgaaatgta cagtctgcca aaatatgaag aagtagatcc aacaccttgg atgacaccat
    49381 tttatcttgt cttcttcggg atgatggtcg cagatgttgg ttatggtttg ctgatgctca
    49441 ttgggacaat actagctcaa aaactacttg ttctaccacg cgggatgaaa cgtttcgtaa
    49501 aattctttga gattctctcg ataccatcga tcatctgggg attgatctac agttcatttt
    49561 tcggacagtc attaccaaaa gaactgtttg gcatccgctt gcctttccca atcctatcta
    49621 cgacggatga cgtcaatacg attttgattt tatccgtgat tttcggtctg atacagattt
    49681 tggttggatt atttgtggca gcaaaggaaa atatcaggag aaaagattac ttgagtgctg
    49741 tcagtgatgg atttgcctgg caagggatcc taattgggat cgttattgcg ttggttggtg
    49801 ccatgttgat aaaaaataca gcatttgttt accttggcgc tgggctatcg atcattgctg
    49861 ccttatgtat cttgatcgtt ccgattatcc aaacgccgtc taaagtcaaa ggtgctgcca
    49921 aaggtgcgta taatctttac ggactgaccg gctatatcgg tgatttagtc agttatacac
    49981 gtttgatggc attgggtatt tcaggcggaa gtattggagc ggcattcaat atgctagttg
    50041 ctttcatgcc gccagctgct cgattcagtg tagggatcct tttgatcatc gctttacacg
    50101 cactgaatat gtttttgaca ctgctaagtg cctatgtcca tggcgcacga ttgcagtatg
    50161 tcgaattttt tgggaagttt tatacaggag gcggacgtgc ctttgatccg ctgaaaacgg
    50221 cggaaaaata cgtcaacatc aatcacaaga aaaaaaacaa taaataattg gaggattttt
    50281 atataatgat ggattactta attagtcaaa atggtggaat ggtatttgcg gtattagcga
    50341 tggcaacagc gacgattttt tcagggatcg gttcagctaa aggtgttgga atgacaggtg
    50401 aggcagcagc agcattgaca acaagtcaac ccgaaaaatt cgggcaggct ctgattctgc
    50461 aattgcttcc aggcacgcaa ggtttgtatg gattcgttat tgcctttttg atttttatca
    50521 acttaggaaa tgacatgtca gttgtacaag gactgaactt tctaggtgct tctctgccga
    50581 ttgcttttac aggcttgttt tcaggtattg cccaaggaaa agtcgcagcg gcaggtattc
    50641 aaattttagc gaaaaaacct gaacatgcta caaaaggaat catttttgct gcgatggttg
    50701 aaacatacgc gattttaggt ttcgttattt ccttcctatt agtgttgaat gcctaagcat
    50761 aaaggaggaa aagcagatga acgccattga aaaaatcatc tctcagatga acgaagcagc
    50821 agagcaggaa agagcggcgt tagaacaaga agagcgaatg aagatcgatc aaaactttga
    50881 acaaaaacgt acgcaagttg aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt
    50941 actcgaaaaa aaataccgtc agctaaggaa tcgccagcaa gtagaagtac gacaagaaaa
    51001 tttgaatgcg aagcaggaat ttttaagacg attgtttgca gatgcggtaa ctgaaatgga
    51061 aaattgggat gaatctgaac aaatccagtt tatcaaaaat gcattgtatt cattaccttt
    51121 gacaggaaaa gtagcattca ttgctggaga aaaatcagca gcctatcttt ctcagacgtt
    51181 gttggatgaa tggaacaatg aacttccctt tatgatggtt ttaagtgatg aaactgttgc
    51241 tgatcaagcg ggttttctga taaacgatca aggagttcag tataattttc ttttcagcag
    51301 ccttgtacaa gatatccaag gaacgatgag ttttgagatt gccaatcaat tattcgaata
    51361 aggagggatc agtgtatgga ataccacgaa ctaaatccct tgattcgcgg aagagagctt
    51421 gaacttttgt ccaatgagac atttgaacgg atgattcaaa caaattctgt cgaagcactt
    51481 ggtgaaatat tgaaatccac gatttacagt ccatatattt atgacggata tgaatatgac
    51541 ttcgaacaga atcttttaaa ggagcagagc cgtctatttc gctggttaaa ggaatcagca
    51601 ccggagcctg aaatcgtttg gatctatacg atgcgttata ctttccataa tctgaaggtt
    51661 ttgacaaaag ctgagatgac aaatcaaaat ctggaccatt tgtatatcga cgatggattt
    51721 tattcattag aaaccttgaa ggatgccatt catacgcaag tttcaaatga acttcctgaa
    51781 agcattatgg acagtatacg agaagtacgt gattattttg aagagtctac cattcttcag
    51841 ggaattgacg tgatttatga tcgacagttt ttgacagagc agcgacggct tgccgataag
    51901 ctaggatatc aagaattgct gacagaagtc attgctttta ttgacttgac caatatcaca
    51961 acgatggctc gaggaatatt gcagaaaaga acatatgggt tcatgacgac ggtactttcc
    52021 agctcgggaa gtatcgaaaa agaagtattt ttgaactttg tagataaaga tatggaagat
    52081 tacacgaatt ttctattaga gacagattat gctgattttc tgcgccctgt catccgtgag
    52141 ggaaatattg atctcgtaaa gcttgaacag ttgaaagatg attatctaag ttcattgtat
    52201 caagaagcac aaacccaggc tttcggacca ctgccactac tagccttttt gaacgcaaaa
    52261 gaggtagaaa gcaaaaacct tcgtttactg gttatcggca aaagaagcca tttcgaatca
    52321 gaaacaatca gaaagcgggt gagacaagtc tatgacgcat aagatcggtg tagttggcga
    52381 caaagattct gttttgccat tcaaattgtt tggctttgat gttcgttatg gtacaaccaa
    52441 gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt
    52501 cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg aacaaatgat
    52561 acctgctatc gtattgattc ccaatcatca aggatcatta gggatagggc tggaagaaat
    52621 tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta tagtatgaaa aggagataga
    52681 aattttgcaa attggaaaaa tcataaaagt ctccggacct cttgtaatgg ctgagaacat
    52741 gtctgatgcc agtatccaag acatgtgtct agtgggagat ctaggagtta tcggagaaat
    52801 tatcgagatg cgtggcgatg tggcgtctat tcaagtatat gaagagactt ctggaattgg
    52861 tccaggcgaa ccggttcgct ctactggtga agctttatct gttgaattag gaccagggat
    52921 catttcacaa atgtttgacg gaatccaacg gccattagat acctttatgg aaatcacaca
    52981 aagtaatttc ttagggcgcg gagttcaatt gcccgcattg gaccatgaga aaaaatggtg
    53041 gttcgaacca acagtagaag ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt
    53101 agaagaaaca aaagttataa aacataaaat catggtgcca aatgggataa aaggaacgat
    53161 caaaacaatc gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca ttgaaaccga
    53221 aaatggtgac aaaaatttga cgatgcttca aaaatggcca gtaagacgta gtcggccaat
    53281 caaagaaaaa ttaaacccgg atgcacccat gatcactgga cagcgagtga ttgatacatt
    53341 cttcccagtg acaaaaggag gagcagcagc tgtacctggt ccttttggtg cagggaaaac
    53401 agtcgttcag catcaaattg caaaatgggc cgatgtggac atggttgtct atgtgggatg
    53461 tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat tgattgaccc
    53521 taatacagga gaatcgttga tggaacgtac ggtattgatt gctaatacat caaacatgcc
    53581 agtagcagct cgagaagctt ctatctatac gggaatcaca attgcggaat atttccgtga
    53641 catgggttat gacgttgcta tcatggcaga ctccacttca cgatgggcag aagcacttcg
    53701 tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag cttatcttgg
    53761 ttcacggctt gcggaatact atgaacgatc tggaagagtc attgcattag gatcagaagg
    53821 tcgcgaagga agtataaccg caatcagtgc tgtatccccg tctggagggg atatttcgga
    53881 accagtcaca caaaatacat tgcgggtcgt aaaagtattc tggggtcttg attccagttt
    53941 agcccaaaaa agacattttc cttcgatcga ttggatacaa agctattctc tttattcaac
    54001 agaagtaggg aagtatatgg atcatatcct tcaagctgac tgggcatcca tggtcaccga
    54061 gggcatgcgt attttgcagg aagaacagca gctgaatgaa atcgtgcgtt tggtagggat
    54121 tgattccctt tcagataatg accgtctgac attagaagtg gcaaaatcca ttcgtgaaga
    54181 ttatttgcag caaaatgctt ttgatgatgt cgatacattc acttcaagag agaaacaatt
    54241 caaaatgttg aagatgatct tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg
    54301 tgcttattta agtgagatca tggacggtac cgtatcgatt cgtgaaagga tcggacgaag
    54361 caaatatatc ccggaaacag aattagaaaa actagatcaa attattggtg aaatcaagca
    54421 aacgatgcaa acgatcgttt cagaaggagg catgagcaat gattaaagaa tatcgtacga
    54481 tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag
    54541 aattaatcga agtccgtatg caaaatggcg agattcgtcg tggacaagta ttggaagtcc
    54601 aagaagataa agcaatggtc caaatattcg aaggaacaag tggtatcaat ttacgtgact
    54661 cttctgtacg atttttaggc catcctttag aactaggtgt atcggaagac atgatcggtc
    54721 gagtattcga tggtttggga cgaccgaaag ataacggacc agagatcctg cctgaaaagc
    54781 atttggatat taacggagaa gtaatcaatc cagtagctcg ggattatcca gatgagttta
    54841 tccaaacagg tatctctgca atcgatcatt tgaatacatt agttcgtggg caaaaattac
    54901 cagtattttc tggctctggt cttcctcata aagaattagc tgctcagatc gcgcgacaag
    54961 caacggtact cgattcatca gatgatttcg ccgtagtctt tgctgctatt ggaatcactt
    55021 ttgaagaagc tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg
    55081 tcatgtttat gaatttagca aacgaccctg caatcgaacg tatcgctact cctcgtatgg
    55141 ctttgactgc tgcagaatat cttgcttatg aaaagggaat gcatgttttg gttatcatga
    55201 cagacatgac caactatgca gaggcattgc gagagatttc tgcagcacgg cgagaagttc
    55261 caggaagaag aggatatcct gggtatcttt acacgaactt agctacttta ttcgaacgag
    55321 caggaagaat ccgcggacta aaaggatcgg ttactcaaat tccgattcta acaatgccag
    55381 aagacgataa aactcatcca atccctgact tgactggtta tattacagaa ggacagatca
    55441 tcttgactag agagctttac aagagtggga ttcagccacc aattgatgta ctcccgtctc
    55501 tttcgcggtt aaaagataag ggaactggac ccggaaagac aagagaagac catgcagcta
    55561 cgatgaatca gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag
    55621 ttttaggtga atctgcttta tctgatgtag acaagatcta tgcgaagttt gctgagcggt
    55681 ttgaaaaaga atatgtcaat caaggattct acacgaaccg ttcgattact gaaacacttg
    55741 atctagggtg ggaactgcta tcgatgttgc caagaaccga attaaaacga atcaaagacg
    55801 acatgctgga caaatatctg ccagaaggga agtgagttct gatggcacga ttgaatgtga
    55861 atccgacccg catggaattg actcgattga aaaaacagtt gacaactgcc acaagagggc
    55921 ataaattatt aaaagataag caagatgaac tgatgcgtca attcatcctg ttgattcgaa
    55981 agaacaacca gctgcgtcaa gaaatggaag agtctatgac agaagcaatg tctgattttg
    56041 tattggcaaa tgcttcgatc acggaacctt tcatcgaaga attatttgtt ttgccggcgg
    56101 aaaacgttga tttgtctatc gtagacaaaa atatcatgag tgtaaaagta cctgtgatga
    56161 attttgaata tgatgaacag ctgacagaca cacctttgga atacgggtat ttgaattcca
    56221 gtgccgaact ggatcggtca atcgatcggt ttactaccct gcttccaaaa cttttggaac
    56281 tgacagaaat cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag acacgtcggc
    56341 gagtcaatgc attagaatat atgacgatcc ctcaattaga agaaacgatc tattatattc
    56401 gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata
    56461 tgggtaccac tgactaagtg gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag
    56521 caaacaaata aagaaacgtt tgccggctgt tcagcttctc gcattaggat tttttctcct
    56581 gattttagtt ggcggcggcc tacttaccct accatttttt agtaatagtg ggcatgggac
    56641 aaattttatc gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac
    56701 attgaataca gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc ttttgattga
    56761 gatcggcggt cttggtttta tgatggttcc gattctatgc tttgcattga taaagaagaa
    56821 agtcagtctc agcacacgta ttatattaaa agaagcgctg aatctagaag aagtatcagg
    56881 tgtgatgaag ctcatgcttt acatcctaaa attggccttt gtgatccaat tgattggtac
    56941 ggttgcttta tcttttgtat ttgtgcctga atttggctgg ttgaaaggta tctggtacag
    57001 tttgttccat gcaatctcta gtttttgtaa tgcagggttt gatttgttag gagacagttt
    57061 agccaatcac cagcaaaatg tttatttgat catggtggta tctgctttga tcatttctgg
    57121 tgggttaggc ttcatcgtat ggcgtgattt actggaatat catcagaaaa agaagatgac
    57181 gattcattca aaaatcgcat taaccgttac aggtaccctt ttattaggcg gattcgttgt
    57241 gttctttttc acagagcgca atggggcaac gctagtggaa gggaatatgt ttcaacgatt
    57301 ggcaaacacc ttttttatga gtgtgacacc tagaacggct ggatattatt cagttgatta
    57361 cctccatatg agccatgcag gtctgatttt aacgatgttt ttgatgtaca tcggcggaac
    57421 atctgggtcg acggccggcg gattgaaaac aactacgtta ggtgtgttgc tgatccagat
    57481 gcgttcgatg ttgaaaggac gtacgagagc ggaagcattt ggccgtacca tccgacaagg
    57541 cgcagtgttg cgggcactta ctttattttt tgttacgttg acgttgtgtg tagtcgccat
    57601 catgatttta tcggtgactg aaacgatacc taaaacttct ggcatcgaat atattgcttt
    57661 tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg ggattgacgc cagacttgac
    57721 tgcaataggg aaattgatca ttatagcgct tatgtacatt ggacgagttg gtgtattgac
    57781 tgtggtcttc tctctgatga caaaagcaca aagaacacaa tcaaaataca aatatccaga
    57841 agaaagtgtc atgattggat aatttcatct tctgacagaa acctagtgat ttagtaaaat
    57901 aagaaaataa aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat
    57961 tttttgaggc actacccgtt gttgaacctc gggtagtgcc tcttttttat tgaaaggaat
    58021 caataagatt tgattccttt atttatcaat aggttataat tcatattttt ttattttttc
    58081 ttttacgata cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg
    58141 aacgaatgca tctggttgcg cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc
    58201 gttagcaaga aagtcattct caattatctt agggtcaatc tcaggatcaa ctggaagaat
    58261 tcgatagtaa gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg
    58321 attggctctt tttgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg
    58381 gaggatttga gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat
    58441 tgcttgttct atcttgcttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc
    58501 agaacaaata aattttgtca ttccagctaa gtagattgtt tggttaggta ataaattttg
    58561 gaaaggagga atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc
    58621 aataacccat agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga
    58681 gatttgaaac cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt
    58741 ttttctacat tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg
    58801 aacgatctct aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc
    58861 tgttgctatt ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg
    58921 aacaccggaa acaatagata gagattctaa ggacggaatg actccttgag tttttaacca
    58981 atttgctgca gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc
    59041 aacaagattt gggttattac tagttgattg aataaacggg acaacgaatt tattacattc
    59101 ttcaaaagaa ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat
    59161 ggtgatggac tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag
    59221 caaacctttc tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa
    59281 ataatcagct agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa
    59341 aatatcttgt tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt
    59401 gtctggtcgc caagttaacg ggtaattgtc ataagaatta ataggcattt tctccactcc
    59461 ttaaaattgt ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt
    59521 tgtgaggagg gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag
    59581 ttttgaagaa gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat
    59641 cagccaaaat cctcgattgg tggaaatttt acctgacgga aaaggcaaaa atatgtttgc
    59701 gatgatgcct gcttatttgg gggagaacag atattttggc acaaaagtca ttaccgcttt
    59761 tccggataat catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc
    59821 tcaaaacgga ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc
    59881 tgctgtatct gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt
    59941 aattggagct ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat
    60001 cactacagtg tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc
    60061 taaaaaacag tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg
    60121 tccagcggaa atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg
    60181 ggtaagggaa gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat
    60241 aagtacatcg ttgatggaaa cttctcaaat ctatgtagat gattactcag ctgccttaac
    60301 agaaagcggg gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt
    60361 tggtacatta ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc
    60421 gacgattttc gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta
    60481 taacaaatta gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt
    60541 gcagatgagg tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca
    60601 gaaggtacga tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg
    60661 aagaaaaacc aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt
    60721 ggtcacgcga cattagcaac agcatttgtt ttatttaact attacaaaat acctgatgaa
    60781 acgattaaat tttctactca aagcggtaat ctatttgtga caagaattaa agattattat
    60841 tacatggatt ttcctagtat tatgccgaaa aaagtgccga tattgtctga atatgaagaa
    60901 gcgattgggg ctaagattaa agaagcctac ttagcacgtg atttattttt cgtattagaa
    60961 gatgaaaaaa cggtggctaa attgcagcca gattttacag ctatcaaaaa ttttgaatta
    61021 ggaataggag tcatcgtgac agcagaaagt gtgcaaaaag actttgtttc tagaaccttc
    61081 tttccaaaat taacaatcaa tgaagatcca gtatgtggat cagcacattc taatcttatt
    61141 ccttattggg cagaaagaat gaataaaaca aaattgtcag cttatcaaat gtcatcaagg
    61201 ggcggggatc tagaatgtga attaaaagaa gatcgtgtga taataggagg aaaagcagtt
    61261 ttgttttcta aaggtacctc gtttatttga tttctttcaa atgaattaaa aataaattgg
    61321 tggagagtgg caaccttata gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat
    61381 ttaatgaaaa gtttgcgtct cattttaatt attggtgttt gttttttatt caatcaagag
    61441 taaaatgccc catactaact atcctcggat gacttcgatt ttataaccat cagggtcaat
    61501 gacaaagtag taagaaggcg cagtgccagg taatcctttt aagtcagtaa tctcaagacc
    61561 agcagcttta tgttgctcgt gtaatttttc tagatcatca gctgctatag cgatatgacc
    61621 atagccgtct cctagatcat acgcaccatg atcatagttg taagtcaact ctaattcata
    61681 gtcatcccca ggaagtgtca aataaacaag tgtaaatttg ttttctggaa aatcacggcg
    61741 gcggctttct tcaaaaccaa atgccttttg ataaaattcg attgatgctt caagatcttt
    61801 cactcgaaca catgtgtgag ccattttcat agattcatct ctccttttcc ttttactcaa
    61861 tcataacaaa aagaagtaag gaaatagttg atttgtgctt gttcatgaga aatttctcat
    61921 gtaaaatgga tagaaaataa aactgttaag gtggaaacta ttcatggata aaccaatttt
    61981 tggtgaaaaa aagcaatttg ctgattatca aacccgttat gcagcatata ttgtcataga
    62041 aagagatcag cagattgcag taatcgaggc tccaaatggt gctttttttc ttcctggcgg
    62101 ggaaattgaa ggagaagaga cgaaagaaga agcgattgtc cgtgagctga tagaagaaat
    62161 gggaattgtg gcagaaattt cttcttatct gggagaagct gatgagtatt tttactcgaa
    62221 ttatcgtgca acttattatt ataatccagg atatttcttt gttgctgatt cttggaagcg
    62281 tataggagaa ccaactgaaa agaccaataa aatttggtgg gtgagccctc aagaagcgct
    62341 tgtgaaattg aaacgtggta gccaccgatg ggcagtcgag agatggatcg aaaaaaataa
    62401 aaactgaaag aaagcgagta gatcagcttt ctctcagttt ttttatttat tacagtacat
    62461 attttttgat tgcttcagct acaccgtgct cgtcgtttga tgaagtgatg acatttgctg
    62521 cttctttgac aagagaaacg gcatttccca ttgctactcc tagaccagca tattcaatca
    62581 tggataagtc gttctcgtta tctccgatag ccatgatttc atcttgatca ataccaaggt
    62641 gctgtgcgag attcgcaact gccgcacctt tacttgcttc tttatttagt acttcgaagt
    62701 aatagggcgc acttttgacc gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa
    62761 ttgctgcatc taaaatttct ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg
    62821 gtgtcatttc ttcaggtgtt cgatatttta gcggcatctt gactaaactt gcttcgtgga
    62881 tcgtataagc gctgatatct cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga
    62941 gatgactgcc gacacggcgt gccattactt cgatctctag gaaatcttca tgtgtcagtc
    63001 cgtgacgggc gatgatttta cctgatttcg tttgttgtac gagtgccccg ttataagtaa
    63061 taacgtaatc attatcttga aaaaggttca actcgtttaa ctgttcctgg acacctggta
    63121 agggacggcc agtgcataaa acgatcgaaa caccttttgc aacagcagcg gcaatcgctt
    63181 cttttacttc ttttgttact tctcttttct catttaataa agtaccatcg atgtcgatag
    63241 cgactagttt gatagacata atttcctcca atatatttta atttatttca atcaatgcgc
    63301 cattacggat atggctggca aattcttgat aagtttcgtc aaataaatca taatgatcac
    63361 gcaaggacgg atcaaccatt tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc
    63421 ccgctaatgc agcaacaagc tgactaacct gtgataattc tatcaaagtt ccgtcatttc
    63481 gcaaaatctc gatttgtgtg cgatgtctgc cttgttctgg gcgataaaaa tcataaggta
    63541 gatcgtagct agaattcaca gcagtgtaat aaacaggatt atatccgacc ttttctacta
    63601 acaaagtgag ttcttggact aattttgaat cccggttgcc agaaaaagta gcagatttca
    63661 gtggtttacg attcaaaaaa cgtttagcta aatcatttaa aataggatca gcagattcag
    63721 accattgagt aaagtaagtg cccaagaccc catcatcaag tttgaggtat tcttctaaag
    63781 taaagcttcc gtcaagaaat ggcagaagta attgagaatg gagttcaaaa ctgctggcat
    63841 cttcttgata caactcgtgt gcgcgatgaa gcagatgctc caaaattact tccataccac
    63901 gggaaacagg atggaagtac acttgcacat acatttgata tcggctaacg atataatctt
    63961 ccactgcatg catgccactc atggaaaaag cgatcccgcc ttcataagga cgaatcacac
    64021 gtaatattcg ggtaagatca aatgttccat attctgttcc agtgaaataa gcatcccgaa
    64081 gcaaatagtc cattcgatct gcatcgatct gactagaaat catttggaca acttgtgggt
    64141 tggggtaagt tttttggatc acactagcta ccttttcagg aaaaccttct tctactcggt
    64201 taagaatctg atacacttct gtttctggag aagtgatgat ctctacagtg atggcttcat
    64261 gattcgtatg gaagatatgt tcaaatgtat gagagtaagg accatgaccg acatcatgca
    64321 acagtgccgc acataaagtc accagccgtt cctgatcatc ccagcctccg ttgcctattt
    64381 tttcttttga aaaatttcga gaaaagatat cgcagattcg tcttgaaatc tcataaacgc
    64441 ctaatgaatg agtaaaacgg ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta
    64501 attgtttaat gcgtcgtaga cgttggactt ctttcgaatt gatcaaatct aagattactt
    64561 gatgctggac atggatatag ttatggactg gatcacgaaa aactttttcc ataggcagca
    64621 tttgatattt ataaggggtt gtcataatgt agctccttta tgttattacg ttgttccatt
    64681 ctctggattt ggttgttcca ttgctccagc tggatttctt tttttagcca gtcggtagaa
    64741 tcctgtaaat cacttgcaga atacagcttt tcaaaagctt ttgcaaatgc ttctttgact
    64801 tcttttacaa aaaggggttg gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc
    64861 actgcaggat agccatttgt accaaagtct tcttttaaag cagattgata aaatcgcttc
    64921 atgacttgcc cacgtttttc ctgattaccg ttgacactta agtacatcat aacagaaatc
    64981 ccatttttga tccgacgctg agcgataccg gcaaattttt tgccatggat gcttaaatca
    65041 taagttccag gacaataaga atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa
    65101 gcgtatttag tcaataggag catttgctcg taagcgtcat ctatggttat tttttttgtg
    65161 tcactttggg ggaaaatcaa agaaatattc aaaattcctt catctgatac gatccctaat
    65221 ccacctgaat ttctgatcac tggagcgtaa cctgcatctt ccagtgtttg gatcccgtca
    65281 cttagaaaag gagttcgaac atccttcata cctaagatga ctgtgcagtc atattgccag
    65341 aaatgtaaaa agatttgctg gtgttcttta gaatattccg ttaatatatc tgttaatgca
    65401 aaaggggaaa agtcgttttt ttgataaatg tgctgatcta gtcgaatcgc agggttcatt
    65461 caaccggctc ctttgtataa attcatattt tctacaatat tatactctaa aatcaaagag
    65521 ttgttttgga aaaaaggttt tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa
    65581 ctaggaacaa tgtagctatg agaagtaaag gagaatgaat aatggaatta tcacaaggaa
    65641 caccgatttc aatcaaattg cgtacaaaag taaaacagca aggggaagtg caagattttt
    65701 attttgattt gaaagggcaa atggtgacaa ttggcgatac gctgtacata cgttataaag
    65761 agattcagga ggagacgggc gatgagattc ctgtaacgat caaattagtt ccagatggtc
    65821 atgtacagct gatccgtgca ggagaaatgc ggatgcgttt gaaatttggc tataaagaac
    65881 ggttagaaac atcctatcga acaccatacg gtatgatcca gattgctact tttacgaaag
    65941 aactgcatgt tagtttgaaa gaccgtccca cagcagggaa ggttcgaatc gattatgatc
    66001 tgttcatggg accggaaaaa atcggcgaat attatttgac cttggatttt acagcgtaaa
    66061 tataaaagaa aagtttgatt ttttgtgtct gattttgtat gataaggagt agactgtgaa
    66121 aggacgtgtc ctgattggaa attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa
    66181 tgatcgaagt agcacacgcg atcttagaac aacgtggcga tgtcatggat ttttctgatt
    66241 tagctaacca gatccaaaac tatcttggga aatcagacag tgaaattcgt gattcactcg
    66301 cacaatttta tactgacttg aatattgacg gcagctttat ctcattaggg gataatcgtt
    66361 ggggcttgcg ttcatggtat gctatcgatt cgatcgatga agaagtaaac catggaatgg
    66421 acgaagagga tgaagatact ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca
    66481 atgatgatga agacgcgatc gattacaatg acgatgaccc agaagataca gatctgacag
    66541 aagaagatga cgatgatctg tttgatgacg atgatgatga agatgaagaa atcgctgctt
    66601 ataattctga cttgcaagaa atcggagcag atgacccgac agatgatgaa gaagacttgc
    66661 caggaaatat cgaagaagat ttaagtatca tagacgaaga cgatgatgat gaagattatg
    66721 aagaggaaga attttctgac tctgaaaaag aataaacatt attttcttac gagaggttgt
    66781 gacagagacg atcagcctat cagaataagg aggtgtgaca cgatttttgt cacaccttct
    66841 tttttgtata taaaaatccc aagtaaatac ataaaaaata aaaaaattga aacttttagc
    66901 gcaccttgta cgtcatatta agtgaggcag aaaggagtgg agaatgattt ttactggata
    66961 taagaaaaaa cggcagatcg aaaaataccg tcacctgctg aatggactca tcgaaactga
    67021 ttatcaaaaa atgttccgta tcacgatgaa ttacgtacat gataaagaag aggccttaga
    67081 tgtgatgcaa gatagttttc ataaagccct gaactatttt gaaaaagaaa aagagatcga
    67141 gcattttagt gcatggtttt atcggatatt gatccgcacg gcattagata gttggcgcag
    67201 acaaaaaagg acgcctgtgg acttatttga aattcaagat atcccccaac agttaccttt
    67261 agaaacttcc gttgcagagc tccatatgat tttagataaa atagacagtc cggaaaaaga
    67321 gattctgatc ctctactttt ttgaaggatt tcgtttgaaa gaaattgcga ctatcttgga
    67381 tatgaatgaa aacacagtta aaacgaaaat gtaccgctca ttgaatgctt tacgtcaaat
    67441 cctagaatga aggagagaag agtgccatgt attcccaaaa agaaaattta aaaaaactaa
    67501 agaaaactta taaagaacaa gcaattccca aagggcttaa agaggagatc cgccagcgtt
    67561 ttattatcga agagcaatca tttataaaag caaagcggag aaagagacgt ctccaagggt
    67621 tcggagtatc tttggctatc gtaatgatta caacaagtag ccttcttttt aatagtcaag
    67681 ttcgaagctt tgcagaagat ttacccatct taggatcagt cattgaattg atcttaggcg
    67741 aacgctttac tgatcgatct gaaaaaatcg atattcaggt accgaagatc aatacacaaa
    67801 atgagaaaga aaataaaaca attcatggat taaatcaaaa atactttcgc gaaggacaag
    67861 cagattttga aaaagctgaa aaagaatatg gaaattttga aacggatcat taccaagttt
    67921 tgggagatta tcagaaaatc gtagacgata accggtttct cgtgattgaa aggcagatca
    67981 cccaaacagc agcagatagc catgttgaaa aaagatacga cacaatcgac aagaaaaaca
    68041 gtgtccagct ctcacttcca cttttattca aagatgacac ctatctatct gttttgacaa
    68101 aagaagtcaa acggcagatg gccgaacaag tcaaggaaga tccatctaag tactattgga
    68161 ctgagcaaga tattcaagaa gggacgatag aaaaaccaac acttgttacg cctacacgaa
    68221 gtttttattt gaacaaagag catcaattag ttttgacttt ttctcaatac gaaattgctc
    68281 ctggttatat gggtacacca gaatttgtca ttcctaagtc cgtgacaaaa acgattttgg
    68341 catcggaaga ttatttggat cattagacga gaagtagttg cgactagcag tttcaatttt
    68401 caagtaagta caagtaaata ataagaagag aaaaagagaa agaagagata tagtgaaaag
    68461 aaattattta gttatcagta ttattgcttt atccgtgctt ttgtttttcg aaattacttc
    68521 ggtggcctcc ttgatcaccg aagtaaaagg acaaacacac tcttcggagc attcgtcaca
    68581 atctttcaaa caatcagaac aatcaaccat taaaagccaa tcaactgaaa cagctaacgt
    68641 caaagaagcc attcaagaat cagcggaaaa ggtacaaagt cagtcatcac aatcaacaca
    68701 agacagtaca aaaacgagcc gagatccggc tgtcaaaagc gtcgcaatca gctttgatga
    68761 tggaccagga gcgaccacaa caccgcagct tctgcgaatc ttaaaagaaa aaaacgtgca
    68821 tgccactttt ttcgtcttag gagaaaatac ggcgcagcat cctgaaatcg tgaagcagac
    68881 agctgaagca gggcacgaga ttggcaatca cacatacgat catcagaatc tggccactct
    68941 ttctgcccag tcgatgacgg aggaagtgac gaaagcagac actgagatca aaaaagtcac
    69001 tggcaaaacc cccacttttg ttcgtccgcc ctatggttct gtcacgaatg taggagcaac
    69061 gatcatccag cgaccgatca tcgaatggtc tgttgattca gaagattgga agacaagaaa
    69121 tcctgatttg attttgcaga agatccaagc aacagtatac gatggggcaa ttattttatt
    69181 ccatgatatc tatcctgaaa cgattcgtgc cgttcctcaa gtgatcgatt atctgaaaga
    69241 gcaagggtat cgcatcacga cagttgggga tttattaggg catccaacgg ctgtcgagaa
    69301 ctattatggc agaaacgatc atcggccagt tcagtaagtg agacaagtat ctttaataaa
    69361 taacacattt taataagtag ttgaacacat aactacctta cttgcaaagg cttaacaata
    69421 tctatacagg tacaacaaaa aactgtccac caattttctt tggcggacag ttttcactta
    69481 aaaataaaga agcaactatt cgtaaattat gctaaaatag cagtagaacg cgtcctgagg
    69541 gaatcgaacc cccgtctcaa gaaccggaat cttacgtgat atccactaca ctaaggacgc
    69601 aagcactggg ataattgtaa ctaaacagat aaaaaattac aaggggattg gacaaaaaaa
    69661 tgaaatttga gcaaaatttt tcacaaaaac agcagcagac acagaaacta gccatgacgc
    69721 aacagttgca acagtccatc caagttttac aatataacag tgaggagctg ttggcattcg
    69781 ttgagaatca agcaatggaa aatccattgg tggaagttgt tgagccggaa tggcagccag
    69841 attacataaa agcttcttca tcttcttatg aaggagaaga gacaaattac ctaaatcaga
    69901 ttcctgatac aaaagggtct ttatttgatt cattgatcga acaagtccat ttgaattacc
    69961 gagatacttt tttgaggaaa atcgttcttt atcttgtaga atacatcgat ttgaacggat
    70021 ttttgacgat tagtttagaa gaagccggaa aagagattgg agctacgcct atccaaatgc
    70081 tcgatgcgtt gactctgatt cagcaattag aaccagctgg agtaggtgca cgtagtttgc
    70141 aagaatgtct catgcttcaa acagaacgag atgattatgc gccggaatta gcttatatcg
    70201 tgttggaaga atgttttgag gaactggtgg agcgaaaatg gaaagaaatc gcacaacgtt
    70261 tttctgttga tctacatgct gttcagcaga tattcgacta tttacaaacg ttgagtccaa
    70321 gccctggccg gattttcgac cgtagcagcg aattgtacat tattcctgat gtccgggtat
    70381 tagtggacga cgataaaaat gtacaagtta tctcaaaccg aaaaaatcag ccaaatatac
    70441 gctttcaaga gagttacttc aaacaaatgt cacaacaagc agataaagaa actgaaaatt
    70501 atttaaaaga acggaaacag gaattcgaat ggttgaagaa aacgatccta caacgtgggg
    70561 atacaatttt gcgggttgct caagtcattg tttcccggca aaaagctttc tttatcgaca
    70621 aggaacgtcc aatcaagcct cttacgttaa aggaagttgc aactgagata gatgtgcatg
    70681 aatccacggt cagtcgtgca gtcaacggga aatatttgga aacagacttc ggtgtatttg
    70741 aattaaaaaa atttttcact acacgaattc ctacgaatag tacagaacag acagaagacc
    70801 tttcagcaga tacagcgaag aaaaagctgc aggaattagt cgatcaagaa gacaaaaaca
    70861 agccgttgtc tgaccagaaa ttggttgaat tgctgaaaaa agatgagata gcgatctcaa
    70921 gacgaacagt agcaaaatat cgtgatttat taggaatccc gagttcatca aaaagaaaac
    70981 gttatgataa ctaatcagtc aaaagactag aaaaagtatt tcactttttc tagtcttttt
    71041 agttgaaaaa cgagagcaaa actgttagac ttacagtcgt gaggttgaga tttcccttta
    71101 ttgatgtgaa attcgaatag ttaatttctt tttttatcgt atatgggtcg ttttaagtct
    71161 acattggacg taaagtgtcc aactaaggag atcgctatgc tggatgaaat aaaaatgatt
    71221 gaatctgttg caccagatat tatcgaagtg ttgcaagaac gctataaaat tttacgaaat
    71281 atctattgga tgcagccgat tggacgtcga agtttatcgg aaagtatggg gatcacagaa
    71341 cgagtattgc gaacagaaac agatcttttg aaaaatctga atttgatcga cacctcaaaa
    71401 agcggtatga cgctgacaac taagggcgaa gaagtttatc aaagccttga aaacttcatg
    71461 gatcagcttt taggtacgca tcaaaccgaa caacagttgg cagaatattt tggtaaccaa
    71521 cgctgtatcg ttgtttcagg aaacagcgaa gagcagacca aagtggctga tgcattcggc
    71581 gaagcgctga gcgaagccct agaccgacta cttccagaag gcgagaatat catcgctgtg
    71641 atgggaggaa ctactatggc tgtggtagcc gaacaattgt ctaatctgga aaataagaaa
    71701 cggcataacc tgtttgtccc tgcaagaggc gggattggtg aagcgataac tgtacaggca
    71761 aattctgtca gtgcaagaat ggctgctaaa gcaaacggta accaccgtgc actttatgtt
    71821 ccggaacaat taagtctggc aacttacaat tctttactca atgagccatc tatccaagaa
    71881 gtcttaaact tgattagtga agcgaattgt gttattcata gtattgggcg tgcattacac
    71941 atggcagcac gtcgtaagat gactgaaaaa gaattagtca tgctgaaaca agcaaatgct
    72001 gttgcggaat cttttggcta ctttttcaat gaaaaaggtg aagtcgttta taaggtccct
    72061 agaatcggca tagaattgca agatttggaa aaagtcccga tcatcatggc aatcgcgggc
    72121 ggaaaatcaa aagctaaagc aattcgagct tatatgaaaa atgcaccaaa acaaacgtgg
    72181 ctcatcactg atgaagctgc tgcaaatgag attttaaaag gggtaaccct ttaaaataaa
    72241 tatttttttg attttcataa ggaggaaatc ttaaatgaca gttaaagtag gtattaatgg
    72301 ttttggacgt attggacgtc tagcattccg tcgtatccaa gatgtagatg gaatcgaagt
    72361 agtagcaatc aacgacttga cagatgctaa aatgttggca cacttgttaa aatacgacac
    72421 aactcaagga cgtttcaacg gaacagttga agttcatgaa ggttcattca acgtaaacgg
    72481 aaaagaagtt aaagtattag ctaaccgcaa cccagaagaa ttaccatggg gcgaactagg
    72541 cgtagatatc gttctagaat gtactggttt cttcacttct aaatcagcag ctgaaaaaca
    72601 tttaacagct ggtgctaaac gcgttgttat ctctgctcct ggcggaaacg atgttccaac
    72661 aatcgtttat aacacaaacc acgaaacatt aactggtaaa gaaacagtta tctcaggtgc
    72721 ttcatgtact actaactgtt tagctcctat ggctaaaaca ttgaacgaca aatttggtgt
    72781 tgttgaagga ttaatgacaa ctatccacgc ttacacaggt gaccaaatga ctctagacgg
    72841 acctcatcct aaaggtgact tccgccgtgc acgcgctgct gctgcaaaca tcgttcctaa
    72901 ctcaacaggt gctgctaaag ctatcggttt agtaattcct gaattgaacg gtaaattaga
    72961 cggagctgct caacgtgttc ctgtaccaac aggttcatta acagaattag taacagttct
    73021 tgaaaaagaa gtaactgttg acgaaatcaa tgcagcaatg aaagaagctt caaacgaatc
    73081 ttacggatac aacacagacg aaatcgtttc ttcagatatc gttggtatga cttacggttc
    73141 attatttgat gctacacaaa ctaaagtaat gacagttggc gacaaacaat tagttaaaac
    73201 tgttgcttgg tacgacaacg aaatgtcata cactgcacaa ttagtacgta ctttagaata
    73261 cttcgctaac ttataagata gacaaacgta caagtgtaaa ttatagacat gtacatttaa
    73321 aagcggggaa gcaatcgcgc ttcttccgct ttttttgata ataagtttat tcaggaggaa
    73381 ataaaatggc taagaaaaca gtgaaagata tcgatttaaa agacaaaaaa gttcttgtcc
    73441 gtgttgactt caacgttcct ttgaaagacg gcgtgatcac tgacgacact cgtatcaaag
    73501 cagcattgcc aacaatcaac tacgttctag aacaaggcgg aaaagcaatc cttttctctc
    73561 accttggacg tgtaaaaaca gaagaagata aagaaggaaa atctttagct ccagttgcaa
    73621 aacgtctagg cgaattatta ggtaaagaag taacattcgt acctgaaact cgcggagaac
    73681 aattagaaga agcaatccgc aacatgaatg acggcgacgt tgtcgtattc gaaaatacac
    73741 gatttgaaga tattgacggt aaaaaagaaa gcaaaaatga tacagaacta ggtaaatact
    73801 gggcttcatt aggcgatgta tttgttaacg atgctttcgg tacagctcac cgtgcacatg
    73861 cttctaacgt aggtatcgct tcaactggta tcccaacagt tgctggattc ttgatggaaa
    73921 aagaaatcaa gttcatcggt gaagcagtag aagaaccaaa acgaccaatg attgcaatcc
    73981 ttggtggcgc aaaagtttct gataaaatcg gcgtaatcga aaacttgatt cctaaagcag
    74041 ataaaatctt gatcggtggc ggaatgactt acacattcta cgaagcaaaa ggaatcaaga
    74101 tcggtaactc tctagtagaa gctgataaag tggaactagc aaaagaatta atcgaaaaag
    74161 caggcgacaa acttgtcttg ccaatcgaca atgtctgtgc accagaattt tcaaatgatg
    74221 ttgaaacaca agtaatcgaa ggagacattc ctgatggttt gatggcttta gacatcggcc
    74281 cagcatcagt aaaattattt gctgatacat tacaaggtgc aaaaacagtt gtttggaacg
    74341 gacctatggg tgtatttgaa atgagtaact ttgctaaagg tacaatcggt gtttgtgaag
    74401 caatcgctaa cttagaaggc gcaacaacaa tcatcggtgg cggagactca gctgcagcag
    74461 cagaacaatt aggatttgct gacaaattca cacacatctc aacaggtggt ggcgcaagct
    74521 tggaattgct tgaaggtaaa gaattaccag gactagcagc aatcaacgat aaataaacga
    74581 ccaagtttgc tcaattctga cgagtaatcg atcaaaaggt gaagggagtt ttcatgactc
    74641 cctagccatt tttcaataaa ttaaggagtg tgttcctcaa tgcgtaaacc tattatcgca
    74701 ggtaactgga aaatgaacaa aactgcatca gaagcaaaag aatttgctga agcagtaaaa
    74761 aacaaaatcc cttcaaatga caaagttgat tcagtcatcg gatctcctgc attattctta
    74821 caagaattaa tgacatcagc tgaaggtaca gaactaaaaa tctcagcaca aaactgctac
    74881 tgggaaaact caggtgcgtt cactggtgaa acttcaccag cagctcttgc tgatctaggt
    74941 gttcattatg tgatcatcgg acattcagaa cgtcgtgaat atttccatga aacagacgaa
    75001 gacatcaata aaaaagcaaa agctatcttt gcaaatggta tgacaccaat cctatgctgc
    75061 ggcgaatctt tagaaaccta tgaagcaggc aaaactgctg aatggatcga aggacaaatt
    75121 acagctggtt tagctgatct ttcagctgaa caagtgtcaa acatggttat tgcttatgaa
    75181 ccaatctggg caatcggtac tggtaaatca gctgacgcac aaatcgctga cgaaatttgt
    75241 ggagttgtac gtcaaacagt tgaaaaactt tatggaaaag aagtatcaga agcagttcgt
    75301 atccaatacg gcggttctgt aaaaccagaa aatattgctg aatacatggc aaaagaaaat
    75361 gttgacggtg ctttagtcgg tggcgcaagc ttgcaagcag attcattttt gtcattgtta
    75421 gacgctgtaa aataattgtt gaaaatttgc gaaaaatgca agaaatgttt gcactaatga
    75481 gcaaaattaa ctaaaatcgt atatgaggga aatcccttag gaaaaaacaa actcaaagga
    75541 gagacaaaac atgtcaatta ttactgatgt ttacgctcgc gagatcttag actcacgtgg
    75601 taacccaaca atcgaagtag aagtatacac agaaagcgga gcttttggcc gcggaatggt
    75661 tccttcaggt gcttcaactg gtgaatacga agcagttgaa ttacgtgacg gagacaaagc
    75721 tcgttatggc ggtaaaggtg taactaaagc tgtcgacaac gtaaacaaca tcattgctga
    75781 agcaatcatt ggctacgatg ttcgtgacca aatggctatc gacaaagcta tgatcgcttt
    75841 agacggaact cctaacaaag gtaaattagg tgcgaacgct attttaggtg tttctatcgc
    75901 tgttgcacgt gcagctgctg actacctaga agtaccttta taccactacc taggcggatt
    75961 caacacaaaa gtattgccaa caccaatgat gaacatcatc aacggcggat cacatgctga
    76021 taactcaatc gacttccaag aattcatgat catgcctgtt ggcgctccaa cattcaaaga
    76081 agctctacgt atgggtgctg aagtattcca cgcattagct tcaatcttga aagctcgcgg
    76141 attagcaact tctgttggtg acgaaggtgg atttgctcca aaccttggtt caaacgaaga
    76201 aggttttgaa gtaatcatcg aagcaatcga aaaagctggc tatgtacctg gtaaagacgt
    76261 tgttcttgct atggatgctg cttcttcaga attctacgac aaagaaaaag gtgtttacgt
    76321 actagctgac tcaggcgaag gcgaaaaaac aactgacgaa atgatcaaat tctacgaaga
    76381 attagtttct aaatatccaa tcatctcaat cgaagatgga ttagacgaaa acgactggga
    76441 cggattcaag aaattaactg acgtattagg cgacaaagtt caattagttg gtgacgactt
    76501 gttcgtaaca aacactcaaa aattatctga aggtatcgaa aaaggaattg ctaactcaat
    76561 ccttatcaaa gtgaaccaaa tcggtacatt aacagaaact ttcgaagcta tcgaaatggc
    76621 taaagaagct ggctacacag cagttgtatc tcaccgttct ggtgaaacag aagattcaac
    76681 aatctctgat atcgctgttg caacaaatgc tggccaaatc aaaactggtt ctctatcacg
    76741 tactgaccgt attgctaaat acaaccaatt actacgtatc gaagaccaac ttggtgaagt
    76801 tgcagaatac aaaggtttga aatctttcta caacttaaaa aacaaataat tcttttctaa
    76861 tgaagataca tgatcgacta ttgaaagata gtcgagtatg atcacttagt taagtaatcg
    76921 aactctctac ttgctgaaat atcagcaggt agagagt
//
LOCUS       DAZKOM010000004        69903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000004 DAZKOM010000000
VERSION     DAZKOM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.004"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8NF_000252"
     CDS             <1..878
                     /locus_tag="ACG8NF_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0470768.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="ACG8NF_000253"
     CDS             complement(1119..2735)
                     /locus_tag="ACG8NF_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0470769.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="ACG8NF_000254"
     CDS             2853..3071
                     /locus_tag="ACG8NF_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0470770.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="ACG8NF_000255"
     CDS             3266..3727
                     /locus_tag="ACG8NF_000255"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0470771.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="ACG8NF_000256"
     CDS             complement(3920..4159)
                     /locus_tag="ACG8NF_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0470772.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="ACG8NF_000257"
     CDS             complement(4183..5706)
                     /locus_tag="ACG8NF_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0470773.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="ACG8NF_000258"
     CDS             complement(5724..5846)
                     /locus_tag="ACG8NF_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0470774.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="ACG8NF_000259"
     CDS             complement(5876..6859)
                     /locus_tag="ACG8NF_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0470775.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8NF_000260"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8NF_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0470776.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="ACG8NF_000261"
     CDS             complement(7053..7430)
                     /locus_tag="ACG8NF_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470777.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8NF_000262"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8NF_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0470778.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8NF_000263"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8NF_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0470779.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10214)
                     /locus_tag="ACG8NF_000264"
     CDS             complement(8448..10214)
                     /locus_tag="ACG8NF_000264"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470780.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="ACG8NF_000265"
     CDS             complement(10174..11928)
                     /locus_tag="ACG8NF_000265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470781.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..12607)
                     /locus_tag="ACG8NF_000266"
     CDS             complement(12038..12607)
                     /locus_tag="ACG8NF_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0470782.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12625..14040)
                     /locus_tag="ACG8NF_000267"
     CDS             complement(12625..14040)
                     /locus_tag="ACG8NF_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470783.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(14051..14719)
                     /locus_tag="ACG8NF_000268"
     CDS             complement(14051..14719)
                     /locus_tag="ACG8NF_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0470784.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14849..15433)
                     /locus_tag="ACG8NF_000269"
     CDS             complement(14849..15433)
                     /locus_tag="ACG8NF_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0470785.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15764..15997)
                     /locus_tag="ACG8NF_000270"
     CDS             complement(15764..15997)
                     /locus_tag="ACG8NF_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0470786.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(16012..16962)
                     /locus_tag="ACG8NF_000271"
     CDS             complement(16012..16962)
                     /locus_tag="ACG8NF_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0470787.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16959..17801)
                     /locus_tag="ACG8NF_000272"
     CDS             complement(16959..17801)
                     /locus_tag="ACG8NF_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0470788.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17803..18522)
                     /locus_tag="ACG8NF_000273"
     CDS             complement(17803..18522)
                     /locus_tag="ACG8NF_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470789.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            19070..19360
                     /locus_tag="ACG8NF_000274"
     CDS             19070..19360
                     /locus_tag="ACG8NF_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0470790.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            19432..20658
                     /locus_tag="ACG8NF_000275"
     CDS             19432..20658
                     /locus_tag="ACG8NF_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0470791.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG8NF_000276"
     CDS             complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG8NF_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0470792.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG8NF_000277"
     CDS             complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG8NF_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0470793.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(22116..22583)
                     /locus_tag="ACG8NF_000278"
     CDS             complement(22116..22583)
                     /locus_tag="ACG8NF_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0470794.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(22703..24241)
                     /locus_tag="ACG8NF_000279"
     CDS             complement(22703..24241)
                     /locus_tag="ACG8NF_000279"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0470795.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24321..25007
                     /locus_tag="ACG8NF_000280"
     CDS             24321..25007
                     /locus_tag="ACG8NF_000280"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0470796.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG8NF_000281"
     CDS             complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG8NF_000281"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0470797.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG8NF_000282"
     CDS             complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG8NF_000282"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0470798.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(26493..27623)
                     /locus_tag="ACG8NF_000283"
     CDS             complement(26493..27623)
                     /locus_tag="ACG8NF_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0470799.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(27623..29806)
                     /locus_tag="ACG8NF_000284"
     CDS             complement(27623..29806)
                     /locus_tag="ACG8NF_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0470800.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(29803..30828)
                     /locus_tag="ACG8NF_000285"
     CDS             complement(29803..30828)
                     /locus_tag="ACG8NF_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0470801.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(30873..31700)
                     /locus_tag="ACG8NF_000286"
     CDS             complement(30873..31700)
                     /locus_tag="ACG8NF_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0470802.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(31697..32764)
                     /locus_tag="ACG8NF_000287"
     CDS             complement(31697..32764)
                     /locus_tag="ACG8NF_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0470803.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(32790..33626)
                     /locus_tag="ACG8NF_000288"
     CDS             complement(32790..33626)
                     /locus_tag="ACG8NF_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0470804.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(33623..34492)
                     /locus_tag="ACG8NF_000289"
     CDS             complement(33623..34492)
                     /locus_tag="ACG8NF_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0470805.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(34562..35845)
                     /locus_tag="ACG8NF_000290"
     CDS             complement(34562..35845)
                     /locus_tag="ACG8NF_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0470806.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(35859..37544)
                     /locus_tag="ACG8NF_000291"
     CDS             complement(35859..37544)
                     /locus_tag="ACG8NF_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0470807.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            37819..38844
                     /locus_tag="ACG8NF_000292"
     CDS             37819..38844
                     /locus_tag="ACG8NF_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0470808.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(39439..43092)
                     /gene="rpoC"
                     /locus_tag="ACG8NF_000293"
     CDS             complement(39439..43092)
                     /gene="rpoC"
                     /locus_tag="ACG8NF_000293"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0470809.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(43171..46788)
                     /gene="rpoB"
                     /locus_tag="ACG8NF_000294"
     CDS             complement(43171..46788)
                     /gene="rpoB"
                     /locus_tag="ACG8NF_000294"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0470810.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            47516..48484
                     /locus_tag="ACG8NF_000295"
     CDS             47516..48484
                     /locus_tag="ACG8NF_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0470811.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            48502..49407
                     /locus_tag="ACG8NF_000296"
     CDS             48502..49407
                     /locus_tag="ACG8NF_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470812.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            49404..50210
                     /locus_tag="ACG8NF_000297"
     CDS             49404..50210
                     /locus_tag="ACG8NF_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470813.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            50367..51335
                     /locus_tag="ACG8NF_000298"
     CDS             50367..51335
                     /locus_tag="ACG8NF_000298"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0470814.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(51652..52176)
                     /locus_tag="ACG8NF_000299"
     CDS             complement(51652..52176)
                     /locus_tag="ACG8NF_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0470815.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            52463..54160
                     /gene="scm"
                     /locus_tag="ACG8NF_000300"
     CDS             52463..54160
                     /gene="scm"
                     /locus_tag="ACG8NF_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0470816.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(54315..55301)
                     /locus_tag="ACG8NF_000301"
     CDS             complement(54315..55301)
                     /locus_tag="ACG8NF_000301"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0470817.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYEGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(55324..55890)
                     /locus_tag="ACG8NF_000302"
     CDS             complement(55324..55890)
                     /locus_tag="ACG8NF_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0470818.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(55904..56320)
                     /locus_tag="ACG8NF_000303"
     CDS             complement(55904..56320)
                     /locus_tag="ACG8NF_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0470819.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(56777..58711)
                     /locus_tag="ACG8NF_000304"
     CDS             complement(56777..58711)
                     /locus_tag="ACG8NF_000304"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0470820.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            59013..59948
                     /gene="pplA"
                     /locus_tag="ACG8NF_000305"
     CDS             59013..59948
                     /gene="pplA"
                     /locus_tag="ACG8NF_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0470821.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            60038..61141
                     /locus_tag="ACG8NF_000306"
     CDS             60038..61141
                     /locus_tag="ACG8NF_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0470822.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            61154..62107
                     /gene="menA"
                     /locus_tag="ACG8NF_000307"
     CDS             61154..62107
                     /gene="menA"
                     /locus_tag="ACG8NF_000307"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0470823.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(62921..64267)
                     /gene="gor"
                     /locus_tag="ACG8NF_000308"
     CDS             complement(62921..64267)
                     /gene="gor"
                     /locus_tag="ACG8NF_000308"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0470824.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(64401..65654)
                     /locus_tag="ACG8NF_000309"
     CDS             complement(64401..65654)
                     /locus_tag="ACG8NF_000309"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0470825.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(65660..66583)
                     /locus_tag="ACG8NF_000310"
     CDS             complement(65660..66583)
                     /locus_tag="ACG8NF_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0470826.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(66804..69296)
                     /locus_tag="ACG8NF_000311"
     CDS             complement(66804..69296)
                     /locus_tag="ACG8NF_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0470827.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(69303..69770)
                     /locus_tag="ACG8NF_000312"
     CDS             complement(69303..69770)
                     /locus_tag="ACG8NF_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0470828.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        20833 a        14355 c        12046 g        22669 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    12541 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    12601 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    12661 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    12721 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    12781 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    12841 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    12901 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    12961 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    13021 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    13081 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    13141 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    13201 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    13261 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    13321 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    13381 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    13441 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    13501 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    13561 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    13621 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    13681 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    13741 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    13801 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    13861 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    13921 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    13981 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    14041 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    14101 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    14161 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    14221 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    14281 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    14341 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    14401 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    14461 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    14521 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    14581 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    14641 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    14701 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    14761 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    14821 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    14881 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    14941 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    15001 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    15061 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    15121 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    15181 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
    15241 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
    15301 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
    15361 cggtcaggca cagttgcctt atcttagttt gctgaaatcc ttctttttca aaatggctta
    15421 gtccactttc tattaacttt tggtgagtta taatataatc tctggtttct atgggtactt
    15481 cctcctaata agatgaatta actttattat cttacacagg acttgaagtt tcaatcgtta
    15541 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
    15601 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
    15661 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
    15721 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
    15781 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
    15841 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
    15901 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
    15961 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
    16021 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
    16081 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
    16141 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
    16201 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
    16261 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
    16321 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
    16381 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
    16441 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
    16501 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
    16561 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
    16621 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
    16681 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
    16741 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
    16801 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
    16861 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
    16921 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
    16981 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
    17041 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
    17101 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
    17161 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
    17221 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
    17281 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
    17341 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
    17401 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
    17461 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
    17521 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
    17581 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
    17641 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
    17701 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
    17761 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
    17821 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
    17881 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
    17941 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
    18001 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
    18061 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
    18121 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
    18181 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
    18241 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
    18301 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
    18361 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
    18421 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
    18481 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
    18541 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
    18601 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
    18661 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
    18721 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
    18781 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
    18841 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
    18901 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
    18961 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
    19021 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
    19081 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
    19141 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
    19201 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
    19261 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
    19321 ctttgacgta gaagatatta aagactttat gaatacttga aattttcttc tcgccgcata
    19381 tactaaaatt agtgaaatat actgccttac tttttttaag gagtgaggat tttgcctaca
    19441 aagctttcaa atgggaaata taaaactaat ctgagatatc caaaaaggtt taaagaaata
    19501 acaggaattg catcagaaaa gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa
    19561 gccgaaaatg atatgaagaa gaaaatagaa aaagttttga gagaggagaa tgcaaactca
    19621 ttagaattaa aaggaaaaat aacgtttaaa aaattttacg aatctaaatg gcttcctaga
    19681 tatgaattag gtcaaacaat tagatcaaac aggcctccta gtgatataac cattagtaat
    19741 acaaaagata tatttcggtt gcatatttta cctatgtttg gtgaatacgc aatgaattat
    19801 ctaaatatca atactgagat aatatcagat gaattaacca aaaaatcgaa agaatatgca
    19861 aatataaaaa tcataaaagg atatgttcgt tccatgttcg atattgcaga aatactaaat
    19921 tatatcgaat ttaatcgaac tactaaaatt attcaaagta tcactgcacc taaaaaaaat
    19981 gctttagaag aaaaaagaat acaagaaggt aagcaagcac tatcatcaaa agaacttact
    20041 aattggatag aagcagtaaa tgatgatttt aataatcatt tacttacttt tcatgattac
    20101 acacttttta tgattaccct gtatcttggt gatagaaaaa gtgagactta cgctttgcaa
    20161 tggaaatata ttgattttga aaaacaaacg gtccgtctta aacatacttt agataaatat
    20221 caaagaaaaa agtttacaaa aggtcgaaaa gataccgtca ttcaagtacc agaagttgta
    20281 atgacattgc taagtgaatg gaaatcggta caagctgatc aattgttgaa attaaaaatt
    20341 aagcaaacgt tagatcagta tctctttact tatactaaac catctggaga agtaaattgt
    20401 cctgttcatg ccgattacct aaactatcga atcaattcaa ttaaaagaag gcatcctgac
    20461 ttagttcatt tatctccaca caaactccgt catacttatg caactatagc tagacaaggt
    20521 ggagcagata tgaatcaaat ttcaaatgct ttaacacact cagatatttc tacaacaaag
    20581 atatatgtga acacaccaga tatagttgat aaggctgttt ttgaagcttt tcagagagga
    20641 ttgaataagt gtgattgata tgtgttctta aatgtgttct taaaaacaaa agaagcactt
    20701 tccaaataat tgaaaagtgc cagaaacgtt gatatataaa ggatattaac gttttgagaa
    20761 ttgtgaagct ttacgtgctt tcttaagacc tggtttttta cgttcgacca tacgtgcgtc
    20821 acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa
    20881 tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac
    20941 gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac
    21001 ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt
    21061 gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat
    21121 atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac
    21181 ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa
    21241 ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg
    21301 acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt
    21361 gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca ctttgtctgc
    21421 attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt
    21481 accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc
    21541 tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt
    21601 attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg
    21661 tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc
    21721 tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt
    21781 cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct
    21841 tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa
    21901 aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggctta
    21961 caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    22021 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatttc
    22081 acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc tggcgctttg
    22141 cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag
    22201 atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt ttggtaaaga
    22261 tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat agtacgatcc
    22321 cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa tgtagaataa
    22381 ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc gttcaaatca
    22441 tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa
    22501 tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg atttaatact
    22561 tctttggttt gttggaattt catctttctt gcctcctaac attttcttac acttaaaata
    22621 atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga ttttcagttc
    22681 gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct cgcagttgag
    22741 caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat ggatagattt
    22801 tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat tctatcaatt
    22861 cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa atcacagctc
    22921 ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt acatcagcca
    22981 tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc tctgtgagct
    23041 gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc ccttctagaa
    23101 tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg
    23161 catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa atcagaccat
    23221 tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga ttttctcctt
    23281 tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc
    23341 cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga acataacaaa
    23401 acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt gctccgctcg
    23461 ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga ttagaaagcg
    23521 gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt tctgctgctg
    23581 tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc aattgctcta
    23641 ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg atatcaaaat
    23701 atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga
    23761 tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt tggctgtttt
    23821 gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca gtctgccgat
    23881 aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct gctcggttat
    23941 ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat atcaggccat
    24001 gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta
    24061 cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt tctgccatcc
    24121 cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct tgggcttcat
    24181 ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt gaaacgttca
    24241 ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt
    24301 tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt atcggatcat
    24361 ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat cctagatctc
    24421 attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt ctctcgatca
    24481 tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac tatttgttgg
    24541 cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc gcaagaaatt
    24601 tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat ttattttact
    24661 tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt ttttggttgc
    24721 aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc
    24781 ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt tcttggagtg
    24841 gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg tttggaatat
    24901 ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg cctttcattc
    24961 cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga
    25021 caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct tgtgacttgg
    25081 agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct gaccctcagt
    25141 attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa gtagcttttt
    25201 cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt
    25261 ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg taatcttctg
    25321 caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc
    25381 tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca tcattatagt
    25441 agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat
    25501 cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct ggatcatatt
    25561 cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca taagttggat
    25621 ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc tgctcttcaa
    25681 atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa
    25741 atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt ttgcttgtga
    25801 cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc aaaacgaacg
    25861 tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa gatttatgtt
    25921 ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac cagcttctga
    25981 atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc ctttgagaaa
    26041 aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga catcaaaata
    26101 acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc
    26161 gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag gcaaaacgga
    26221 tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct cagcaaaggc
    26281 tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca atcgctctag
    26341 tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga atggaatgga
    26401 cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt ctacaagaac
    26461 gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg aaaatgaacg
    26521 acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt ggcttgctgt
    26581 aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga aatgccaaca
    26641 tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac aggaacgccc
    26701 tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg tccatctaga
    26761 cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa
    26821 tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca
    26881 cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata
    26941 taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt
    27001 ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg
    27061 acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt
    27121 ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa
    27181 cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg
    27241 atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg
    27301 gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc
    27361 atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat
    27421 gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc
    27481 gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct
    27541 tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa
    27601 tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca
    27661 gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta
    27721 ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag
    27781 aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg
    27841 taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa
    27901 ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata
    27961 aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac
    28021 tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg
    28081 gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga
    28141 cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac
    28201 gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa
    28261 tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt
    28321 tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca
    28381 gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc
    28441 tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt
    28501 catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat
    28561 actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa
    28621 cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac
    28681 cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt
    28741 gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa
    28801 actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat
    28861 aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca
    28921 taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt
    28981 ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa
    29041 tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt
    29101 gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga
    29161 tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat
    29221 tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga
    29281 tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg
    29341 tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga
    29401 tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga
    29461 cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt
    29521 ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac
    29581 cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg
    29641 ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt
    29701 cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat
    29761 acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc
    29821 tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca
    29881 gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact
    29941 tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac
    30001 atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac
    30061 attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc
    30121 gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca
    30181 tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac
    30241 gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata
    30301 atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac
    30361 accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg
    30421 attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg
    30481 cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg
    30541 tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa
    30601 gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac
    30661 tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac
    30721 tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg
    30781 tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt atttctccct
    30841 cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga
    30901 cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca
    30961 ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc
    31021 gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct
    31081 gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct
    31141 tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag
    31201 tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa
    31261 ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat
    31321 tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga
    31381 taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt
    31441 gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca
    31501 ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt
    31561 gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca
    31621 aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga
    31681 tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt
    31741 cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa
    31801 ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt
    31861 cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag
    31921 caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa
    31981 aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct cttaaagaag
    32041 cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca cgtttatctg
    32101 tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg taatcggcac
    32161 cgtatttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg atggtagtag
    32221 cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg
    32281 ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg aattgagcag
    32341 gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt agtttataat
    32401 tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag actaactctc
    32461 ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca atcatatttc
    32521 ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt ggcaagaata
    32581 ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta aagtcgacaa
    32641 tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg ttcttttcga
    32701 tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca actaattttt
    32761 tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc gactaaatca
    32821 cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc ggtcaccaat
    32881 gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg
    32941 attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa ataatcattc
    33001 cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc caatggtata
    33061 atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc ttcttcaata
    33121 tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg aagtgtttga
    33181 acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc ggaaatgatt
    33241 ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta aaagctagca
    33301 ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc gctgatccca
    33361 atcactactg cagttacgga ggctatcaag gagatcacta aactattttt gaaatacatg
    33421 atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt ccattctttc
    33481 ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc
    33541 atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc gtaaaataag
    33601 attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag agcaagacga
    33661 ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca agatcattga
    33721 acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc gtcagtagat
    33781 agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca gttgctagta
    33841 ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt gcaccgtcca
    33901 tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa
    33961 acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag ctagcaactg
    34021 gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact aacagatcat
    34081 tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa gaaatagatg
    34141 gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg aacttgcggt
    34201 taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact aagaatgtgt
    34261 aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt tcaagataat
    34321 tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg aaaaaactga
    34381 tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc atgcttggtg
    34441 ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc attgaaacct
    34501 ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc agaacttctt
    34561 gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg tttccgacag
    34621 ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc attttcgtga
    34681 aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta gcaatctcag
    34741 atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc agttcgacta
    34801 cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt tccatgtttt
    34861 tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc tcgtctagac
    34921 cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt tgtgttggga
    34981 tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa atgtaagttg
    35041 aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct gtcacatcat
    35101 tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct tctctcatct
    35161 tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca tttgacaaag
    35221 caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata gcaattccat
    35281 attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca tcccaatttt
    35341 ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata ccttgaaccc
    35401 agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca aggtttttgg
    35461 ctccgtcata ataattatct tctccagctt cctcgataac gttttggact gccgtctgat
    35521 caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc attacttctg
    35581 gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat tcttctaccg
    35641 gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc agttcattga
    35701 tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg
    35761 attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta gctagcagac
    35821 aggtagtaac aataattttt ttcatttttt tccacctctt attttattat tttcagccac
    35881 atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat aatttgtcca
    35941 gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg attggaaaaa
    36001 ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat aaaagaatct
    36061 gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt ttttcgaata
    36121 gatagcatct gggtcaattc accatatact gctgttctta atggatctgt ctgcaactct
    36181 ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc tcctgttttt
    36241 tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat tgccggcacg
    36301 cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct ccattccgtc
    36361 gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata tggactggtc
    36421 gttccatctg ggtttttctt gtagtttact cgggtaccct ctttcttcag ctgttccact
    36481 aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc gataccgtca
    36541 tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac
    36601 cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa aggtggtagc
    36661 gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact gatattatct
    36721 ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc atcagcgatt
    36781 gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga agttcctagt
    36841 tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt ttctaagtaa
    36901 aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt caagtctact
    36961 tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt
    37021 gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc cagtggcaaa
    37081 tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa ccaagtactt
    37141 tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct tctcatttct
    37201 tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac aacggagaaa
    37261 ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa tgtatctgcc
    37321 aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat
    37381 tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg
    37441 cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt tttctcagac
    37501 aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt cccctttctt
    37561 ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg tcaatagcat
    37621 ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat
    37681 taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca
    37741 aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa
    37801 aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc
    37861 tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa
    37921 gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga
    37981 ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc
    38041 tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat
    38101 aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg
    38161 aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa
    38221 acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg aaaagtcgga
    38281 aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg
    38341 gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa
    38401 gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta
    38461 gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca
    38521 atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta
    38581 aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt
    38641 tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg
    38701 atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc
    38761 caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat
    38821 tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa
    38881 gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta
    38941 gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt
    39001 taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt
    39061 tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt tggggatact
    39121 tatctctaaa acataaatag aataacagac atgtctactg gttttgtttt attcgaggaa
    39181 ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc atcaatttat
    39241 tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt ggaagatgat
    39301 cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc cacttttctt
    39361 ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata atttgtcaca
    39421 acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg cagccatttg
    39481 cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt cttttggttc
    39541 catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta taataacatt
    39601 ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg tcaagacacg
    39661 agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg atgcttttgt
    39721 gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta gcgtattgta
    39781 gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat ctgtatcacc
    39841 tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga tatgcttgtc
    39901 gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca agtagttttc
    39961 aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa
    40021 tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg tataaggaac
    40081 agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac gtgttgcagg
    40141 atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt gccctttcgg
    40201 attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat cgtctccggc
    40261 aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac cgattgattg
    40321 agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg caaggttgcg
    40381 tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag aacggattgt
    40441 cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa tcaattggtt
    40501 tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg tgtaacgtcc
    40561 cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg cttggatttc
    40621 aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg ccacgtcaac
    40681 cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt
    40741 acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac ggaagttcga
    40801 aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac gcattccggc
    40861 aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca tgaagattgg
    40921 atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg cagcattcca
    40981 aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac ggaattgttt
    41041 tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt cattcagtac
    41101 actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc
    41161 tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga agacttctgc
    41221 gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct ctttgatatg
    41281 tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt
    41341 gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt tgaagatgat
    41401 cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg gtttttcacc
    41461 taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt tgcgccatgc
    41521 gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc cttcttcttc
    41581 cattgtcaag tagtagttac ctagaaccat atcttgggaa ggagtaacaa ctggtttacc
    41641 atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt ctgcttgtgc
    41701 ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg cattataagc
    41761 ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg gttcgaatgc
    41821 ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt gttccttgat
    41881 tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac gtttcgcatt
    41941 tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg gtttgaataa
    42001 ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag gtccaacaac
    42061 gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac ggaaacgacc
    42121 ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg gtccagtaac
    42181 tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca gcatacgttt
    42241 ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca aacggttatt
    42301 acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac gtccaccttc
    42361 taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca taaccatcca
    42421 gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc ggattgcacg
    42481 tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg cttctgcatc
    42541 tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag cttggaattc
    42601 ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt gttttttctc
    42661 taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga tttcttccaa
    42721 agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt taaagtacca
    42781 gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc gaactttcga
    42841 acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga tacgtttgta
    42901 ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac agaacaaccc
    42961 ttcgcgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta cttcaccgta
    43021 agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt cgaatttatt
    43081 tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa gacggctgat
    43141 cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg cggtttcaaa
    43201 gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct tttctaattc
    43261 tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca aatcgtcgtc
    43321 ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga cacgcatgtc
    43381 cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta cacctggttt
    43441 tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga ctacgtcatc
    43501 tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt ccagtgccca
    43561 tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca atggttgttg
    43621 tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc
    43681 aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt cacctgtacg
    43741 tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa ctgtttccca
    43801 tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac ctaactgacg
    43861 tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg aaggcacccc
    43921 taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca tatcttcttc
    43981 cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt tatccccttc
    44041 atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc ctggtgacaa
    44101 ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc cgccgccatg
    44161 tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa
    44221 tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc ctacaagcaa
    44281 atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta aatcttttaa
    44341 tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta attttgtatc
    44401 gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat cttttaccaa
    44461 acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca tgaaaccaac
    44521 taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt ctgccagagt
    44581 atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt agcttgttcc
    44641 ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg
    44701 tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac ataacaaagc
    44761 agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg gtgcttgagg
    44821 attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac ggttggagtc
    44881 atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt taggtgatac
    44941 gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag cacgagccat
    45001 aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt gggctacgat
    45061 gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc
    45121 acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt tcgcataact
    45181 ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg gacacatacg
    45241 gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc
    45301 gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg
    45361 atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg ccacaactgg
    45421 acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg acattctttc
    45481 acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta aaagttcacc
    45541 gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc cgataccttc
    45601 catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa ccgtttttac
    45661 agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt ctcttggtga
    45721 aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt ctgatggata
    45781 gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca tgacttggtg
    45841 agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc
    45901 agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta ctttgtaacg
    45961 accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca gcaagttacg
    46021 tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc
    46081 ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa
    46141 tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg
    46201 aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag agatgtcttt
    46261 tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag aaccgaatcc
    46321 ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac ggactaactg
    46381 agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg tcattaatgg
    46441 gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg
    46501 caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt ctgctactgt
    46561 atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc cattgaagtc
    46621 atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga accattggta
    46681 agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga ttcttgcgaa
    46741 acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc tcttcacccc
    46801 tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt tcaaatatta
    46861 tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa
    46921 cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat taaagccgaa
    46981 acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac ttcatatact
    47041 acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt
    47101 ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt gacagacctt
    47161 tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg cgaaccaaca
    47221 tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt agataggttt
    47281 gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat ggctaaagaa
    47341 ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc agtgaaaata
    47401 caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt tcaatccatt
    47461 tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa
    47521 aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag gttgcgcaca
    47581 aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat tgcaacttgt
    47641 acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag cagaagaagg
    47701 gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca atcaagacaa
    47761 cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac ttggaatcgc
    47821 cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga ctgtcacagc
    47881 ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg gcggcaatgt
    47941 gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt taaatatcgt
    48001 gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact ctaagatcca
    48061 agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc
    48121 aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg tagatggaat
    48181 ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa
    48241 agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc
    48301 aacagtagga atcgactacg aaagcttggg taaacaaaca gggatcatgg cagcaaaaat
    48361 cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta
    48421 tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca aagaagctga
    48481 ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc atctgcatcg
    48541 caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg tatcttggac
    48601 attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc ggcagtaagt
    48661 attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg gggtgcggca
    48721 gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg
    48781 atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc gccgaacatc
    48841 tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat aacaaaagac
    48901 aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt acttgttttg
    48961 ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct ggcaatgagc
    49021 gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat ttctaatgga
    49081 tgcattgctc tttctggctc cttgcttgcg caaaacaacg gctttgctga tttaaattct
    49141 ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt tttgttacgt
    49201 aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat ttatcgtttt
    49261 attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact tgcttctgct
    49321 ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca tttacctcaa
    49381 aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat ctccatcata
    49441 cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat ctttccttag
    49501 atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct actttgctga
    49561 acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat ggaaaaaata
    49621 tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc caagatccta
    49681 aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct atgaaacgag
    49741 gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc
    49801 agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt
    49861 ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt ccagaactga
    49921 tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg gtgatggaat
    49981 tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact catgacatgg
    50041 aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg atcgtcgtag
    50101 atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg ttgttcaaac
    50161 aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa tcagaaagag
    50221 gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct ttcgtttttg
    50281 ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc ttcctgatag
    50341 aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt caattattga
    50401 aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa gtttcccgga
    50461 caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc
    50521 ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc cagcaagagc
    50581 tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa tctacgatga
    50641 aagatgtcaa actggcagca gctcaaggag attctgtttc gaagttattt gttgctgata
    50701 cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct ggacgaggaa
    50761 tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct caatatacga
    50821 tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga
    50881 tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag
    50941 cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc gggcttaatt
    51001 tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc tctgtctttc
    51061 ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg caagaattgt
    51121 tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat
    51181 tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa
    51241 tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg
    51301 gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca aaaggacacc
    51361 tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct tattctatca
    51421 gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata aagcatggta
    51481 cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac acgatcaaaa
    51541 gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa cgataaaaac
    51601 aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc cttaatctct
    51661 taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag catcatcaat
    51721 aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt caattacttc
    51781 atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag cagctcctcg
    51841 caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt gttccagact
    51901 atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt cccagtcttg
    51961 actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta attctcgagc
    52021 ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt ccactggaaa
    52081 acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg
    52141 atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt tttatttatc
    52201 ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca cccttatttt
    52261 ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg
    52321 aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct ttaaataaac
    52381 aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata ggaaaatgtt
    52441 tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt
    52501 gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca ttcctaaaga
    52561 ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa ttgctagtca
    52621 attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg gaaatatagc
    52681 aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag gtttattgaa
    52741 ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag gcatacctac
    52801 aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag cacttcaaga
    52861 tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg atttttataa
    52921 agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat tgaatgtagc
    52981 agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag attttccaga
    53041 catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt tattggtaaa
    53101 tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc caaatgatca
    53161 agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt caccaatcaa
    53221 atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata tctcttggaa
    53281 ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa caatccttgc
    53341 gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag gaacaaatgt
    53401 gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca gcgacaatgg
    53461 tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg atagctcaga
    53521 ttcatcaact tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggatac
    53581 atcagcttca agcgacagta cagatactac ctctaccaca agtgatagca cggacacatc
    53641 agcttcaagt gacagtacag atactacctc taccacaagt gatagcacgg acacgtcagc
    53701 ttcaagcgac agtacggata catctaccac aacaagtgat agcacggaca cgtcagcttc
    53761 aagtgacagt acagatacta cctctaccac aagtgatagc tcagattcat ctaccactac
    53821 aagtgatagc acggacacgt cagcttcaag tgacagtaca gatacatcct ctaccacaag
    53881 tgatagctca gattcatcaa cttcaagcga tagtacagac acgtcgtcta ccacaagcga
    53941 tacacgtcat gcaagtgtct ccttctcaaa aacaagttcg gataacagga cgaactttaa
    54001 cttaactaat aaaacaagca atgacaatgg aaacaaatca aaaagagcac ttcctaaaac
    54061 tggaagtcaa tcgaataatt ggataacact cgcaggcgtt atattacttg taatcggttt
    54121 aagaatcact tttatgagtt ataagaaatc gaaaaggtaa aaaaatattc aagataaaaa
    54181 agagcaacgt cgctaacgga cgttgctctt tatttttgct ttttatcata aatacaagaa
    54241 ctacaattca atttgtcaaa aaacacgtag atttccttct gagaaattta tttacgtatc
    54301 gaacctttcc ctattcagtc tttgcgatcc aaaatcagtt gggtaaggga aaagagattc
    54361 tcttttgttc gaagagaggt ggcaggtaat ttctttattt ccttcaaagc cttttctgtg
    54421 taagaggccg ctaacttcct agcttcttcc acgcctcctg tatgttcaac gattttgcgt
    54481 acggcgtcga catcttcttc tgtcattgcc gctttcttct ctaaataagg tagtagcttt
    54541 ggtttgtttt tttgcaaaga gtagatcaac ggtaaagaat agaccccttg acgcatgtct
    54601 tctagtacag gtttgccgat ttcctggctc tcttttgtat aatctaaaat atcatcgata
    54661 atctgaaagg ccatcccgat agaaagtccg atatctcctg ctttttttgc gaagcgctcg
    54721 cttgttccac tttcaaaagc gccaacagaa caacttaatt ggaacagttc agctgtctta
    54781 cctgaaatat tttccaaata ttcttttaca gaaagttcat aattatagcg atcatccatc
    54841 tgccctaatt cgccattcaa tactttttcc atactgcgag aattcaattg gagacttttc
    54901 aaggaagtgg agtaatcaga caatagttta aaacagcaga caaataaata gtctccttca
    54961 tagacagaaa cgacattacc aaactgacta cggatattag gctgaccacg tcgcagatcc
    55021 gcttcatcaa cgatatcatc atggatcagt gtggccgtat ggagcatttc gatagaagct
    55081 gccaaagcga tggctttgtc tcgatcttgc tctggtccaa attgtgagaa caatagctga
    55141 taggcaggac gcaacagctt tccgccggca tggatcgttt ctttgattgc ttgttcgaca
    55201 ggtttgtttt tcaggcggac agtttcttcc atccgtttta gtgtttctgt caattcttta
    55261 cttaaagcag gatagttttc ccacattgga tgtagcttca taaaaaaact ccttgtgtga
    55321 cgattatttt ttggtaaaca ttgggctcca tttttgtttg gttccctgct cataatcttt
    55381 ttggaatcgt tgaagtgtct gcacatgtcg taaaagataa ttcgctgcga tcccgattgc
    55441 aatccctgat aaaatcccta aaactgaaag gactggcaag tacaacatga ctgtccatga
    55501 ttgtgcaatg aaagaagcca tcaaaagctg tccgacatta tgcatgaaac cgccggccgc
    55561 actaattccg atgatactga cacgttttgg tcctaattgc ttgaccaata acattccaaa
    55621 ataactaagc aaagcaccac tcatactata aaggaatgtg gaaatcgttc cgcctagcaa
    55681 agtagtcagg aatagtcgta gccaaagtag taagaagctg tctcgttttt tcatggtaaa
    55741 aatggcaacg atcgtgatta gattggctaa gcctagtttt gcaccaggcg caaaagaaaa
    55801 aggataaggg atcatatttt ccaataaacc aatgatcact ccttgtgcaa caagtagcga
    55861 aatataaatc attttttgta atttactcat ggtgtgtgaa ggtctaataa atcagactgt
    55921 cttcgtcacc tccatctgtt gaacggactt cgatcactaa tttatgtggc aaacagacga
    55981 tggtttcgcc atttttcgtt gcccagcctc gacgtacgca gatctggtca tcgcaatctg
    56041 cttcttttat ccgtactcga tcgccatcta tctcgatcaa gttataatct ccgtgttcat
    56101 ctttgtaagt atatgtgttg ctctcttctc cagccatcaa agggaatgtt ttgatttctg
    56161 tgccatccac acgcagtacc gcttcttttt ctgctgttgc ggtgttttgc tgaagaccaa
    56221 aaatcatgac tggtaaaaaa gaaagaacag tcaaaagcaa aataataaaa acatcccaag
    56281 gtttgagcag acttttttca gcaaattctt taaggctcat ttttgttctc ctttttccag
    56341 attaactcac ccaattttag cataaaaaca agaggttgtg acacctgtgt cttgctttga
    56401 gtaataagaa cgaagaatag aaaacagctt tccatgtttt ctaattcttt gggcttatta
    56461 ccgagaagca gcaggtggaa caccgtttat aacaagaggt tgtgacagaa gtgggcaact
    56521 tcaagaaata agaaggaatt tacgaaaatt acttttcaag tttttgtgaa atttcggctt
    56581 atttttcgaa gaagctactt ctggaacacc gtttataaca agaggttgta acagaggttg
    56641 tgacacctgt gtcttgctca tggaggctta tctcgggcat cttattcctt taattttaca
    56701 taaaaaggag gctgtgacgg tttgtctcag cccctttcta ttgaaatctt gcattcaaat
    56761 aacgttcttt atctatttat ttcttgccgt actgggaact tggtctaccg taccaccaat
    56821 tacctagtgt acgttgtagc catggaatta cccagcgatc caacccaatc gcacgtccgg
    56881 aaccgttcat caatgcgaaa gcaacaaaaa tgaaccaaat gtttacccag taaaacatgc
    56941 ctgataagca gaacgtgatc accaatacaa tcgttgtcgc actgcatagc catgtgaata
    57001 atccagcaat caatgctaat gccaaggcaa tttcaaagaa taccatgaat ttttggaaga
    57061 acattgctac atcattgttt ggcatgatca cttgcatgat ctttgtgaac cagtctggtg
    57121 acttctcaat cacagccatt ggttcttcac cgtatgcata actcaaacca aaatgtgcag
    57181 cttgagtcgc tgcttctccg gcacttgccc ctgctccgct ggcagctgtg gttgcatcac
    57241 cagcagcaga agcaccactg gcaacatctg ttacattgta ttcccatgtc caagggaaca
    57301 cactgtttcc agtgaaccat gatccttcac caaaccaagt acttggtttg aacacttcac
    57361 cattcccaat gatttttttg gaagcttcaa cgaaccaaac taaaccatag aaaatacgaa
    57421 gtggcacact ccataagaca ttgccgtaac gagaagaatg tcctcgagtc acgttgcgat
    57481 catctttgat atggaaaaac tcatgcatta aatattggaa catataataa ccagaacgaa
    57541 tatcaaagaa gtatttcaaa ttcacgatat gtttcattac gatagctaaa aatccgctta
    57601 aatggatctt atcaaatagg ttagctaccc cccatttggc accaatagaa accataaatc
    57661 cttgatagtt tcctttaaat ttatgctttt ctccgccttt gatcgaagcc acgatattcg
    57721 ctgcggctgt gtgtcctgtt tgttcagcag cttgaacgat ctgcggtgtc ggtgttcctg
    57781 ggaattcttc ataaaagact aaatcaccaa tgatatagat gtttttatct tcgtagcctt
    57841 ttgcctgcat atattcattg gcgatcaacc gctgtccgcg tgctgattct aatccaaagt
    57901 cagcagcgtc agaagttcct ttgacaccag ctgtccaaat cagtgtgtga gtcggaatgt
    57961 tgcgtccgtc tttcaatttg atatgatcag cagccacttc tacgattggt gcattcaata
    58021 ataattccac gttgtgctta cgcaaatagc gttcagcttt tgcagcatca tttcgatcca
    58081 acatattcaa gatcgtcggc attgcctcta cgaccatcag tgtgatttcg tttggatcta
    58141 atttataatc tttggctagg cgatttttcc agtcgatcag ttccccaatc atttcaatac
    58201 cggtaaatcc agaaccacag acaacgaaag tcagcatggc tttacgcagt tcatcatctg
    58261 gttccaatgc agcttttgct actgtcttct caatatgttc ccgaatcctt acagaatctt
    58321 caaatgacca aagggtgaat ccgtgtgctt tgacaccagg tgtaccaaag tcattcggct
    58381 ctccgcccat tccaaggatc agctgatcaa aaggatagga acctaatttg gtcttgacga
    58441 tttttcgttc tttatcgata ccgattacag tgtctgtgac aagttgtaca ttcttttgac
    58501 gagcaaacaa atgctgcaga tcgtattgga ttgcttctgg ttctacacgt ccgccagcta
    58561 cttcatgcaa ctcggtcatc atagtatggt acgaatgacg atcgatcaga gtaattgcta
    58621 catcttcttt tttcagtttc tttgccaagt attttgtagc tgaaacaccg gcatatcctg
    58681 ctcctacaac gacaatattt tgctttgcca ttgatcatcc gctccttata gactatctat
    58741 ataatgaata tatgataatt cttttagttt gatacttatt acacaatctc catatattat
    58801 atagatgttc tgttcttttg tctaactcct tttggatttt tttcaaattt taaaataaag
    58861 cgttttttca cgaaatttgc tgaaagaaaa cgtttgaaat attacatggg ttatttatca
    58921 aaaatagttg acttataggc taaaaagatt agaatgtata atgaatcaca aagaaaaatt
    58981 gtggactaga aatggaatgg tggggtatta aaatgaagaa aaaacgtttt gtaactggat
    59041 ttgccgtatt agcattttca gctctagtgt taggcgcttg cggcgcagat gacaacggaa
    59101 gctcaaactc ttcttctgaa tcatctacag cacaatcttc gactgcaaaa agcacaacag
    59161 aatcatctgc aaaagttgta gctggcggag atttgcaaga cggcacatat aaattagaag
    59221 aaaaaaatta ttcaaatggt taccgtgcag tctttgaaat ggttgtaaaa gacggcaaaa
    59281 tcactgaatc aaaatatgat aacgtcaatg aaaacggcga atcaaaaaca aaagatgcag
    59341 aatacaacaa aaacatggaa gcaaaatctg gcacaaaccc agaaaaatac attcctgagt
    59401 tgaatgacca attcttaaaa gcacaaagcg caagcggtgt ggaagtcgta actggagcta
    59461 ctcactcatc tgaaagcttc caaaactacg cacaacaatt gatccaagct gctcaagctg
    59521 gtaacacaga tacaatcgaa atcgacaatg gtgctgattt gaaagacggc acttacaagc
    59581 ttgaagaaaa gaactactct aacggctacc gtgtgcaatt cgaaatgaca gttgctggcg
    59641 gcaaagtaac tgaatcaaac tttgattata tcgacaaaga cggtaaatca aaacaagatg
    59701 atacagaata taatgaaaat atgaaagcaa aaagcggtac agaaccaaaa acttacatcc
    59761 ctactctgaa cgacgaattc gtcaaagcaa tgggcgaaga agatggctca cctgctaatg
    59821 tagaagttgt tacaggtgct acacacagct cacattcatt catcatgtac gcacaacaat
    59881 tagtgaatgc agctgaaaaa ggcgacactc aaacaatcga agtagataat atcgtaacag
    59941 aaaaataatc acataatcat actaataaaa gttggagggt gggacgatag ccagattagg
    60001 cagtcgtctc acctttcaaa atgaagagag gaaaaagatg cggaaaaaac tactattttc
    60061 cttaatcaca ctgggcatct cttttatggt gctaactggc tgcagcaact cagcaaataa
    60121 aacaaccact gaaagttcag cagctaccaa gatattgaaa gatccatatt ctgatgaaca
    60181 gttcttgtta gggacttacg ttcgtatccg tgtgtatgat gaaggcaaag aatcagcatt
    60241 gaaacctgcc ttcgatcgag taaaagaact tggtgataaa atcacgatca atcaaaaagg
    60301 ttctgagatc gatgaagtga atgagcaagc tgggatcaaa ccagtaaaag tttctgatga
    60361 cgtctacacg ttagtcaagc gggcctacga atatagccaa gattctcaag gcggattcga
    60421 tatggcgatt ggtgcaatca cacaattatg gcgaatcggt tttgatgacg ccagaaaacc
    60481 ttcccaagaa gaaatcgatc aggcattgaa attagtggat tatcataaga tcgaactaaa
    60541 tgacaaagaa aaaactgttt atctaaaaga aaaaggaatg atcatcgatt taggtgcaat
    60601 cgccaaaggt tacattacgg acgaagtagt gaaagtcttg aaaaaacaag gagtgaccac
    60661 tgccatcgtt gacttgggcg gtaatgtcta tgtcttaggc cacagcccaa gaggcgaaaa
    60721 ccaagactgg acagtcggca tccaagatcc gaacatggct cgcggatctg ttttaggttc
    60781 tattaaagaa cggaacaaaa cattagttac ctctggaatt tacgagcgtt atttagaagt
    60841 agatggcaaa aaataccatc atttgtttga tccaaaaacg ggttatccgt tcgacaatga
    60901 tatcgcaagt gtcacgatca taacagataa atcaattgat ggtgacgggt tgtctacagc
    60961 agtcttttca atgggtgtga aaaaaggact ggaatacgtc gaaagcgaat tgaataacgg
    61021 gacggaagcg atcttcgtga caaaagacga taaagtatat gtgaccgatc caatcaaaga
    61081 caccttcaaa ttatcggagg actcccatta cacgatgggt gaccgttctg aactaaaata
    61141 aggagtacga accatgacac taaaaatctt tttagaagta gttgaaatac gaacaaaggt
    61201 ggctagtgta tttccttttg cggtaggggt attattctct atcgcttttt ttcatgagat
    61261 acattggcta aacacttttt tattttttat tggaatgcta gtttttgatt tagcgactac
    61321 cgctatcaat aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga
    61381 aaatgtcatt ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat
    61441 gattgcattt gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact
    61501 tttgatggga tttatctgtt gttttattgg agtattctat acattcggac cgattccttt
    61561 atctcgaatg cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc
    61621 catggtcgtt tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc
    61681 atctggacaa tttgctttgg taggaaatct ttgggctata gcagcaatcg tattagcttc
    61741 cttacccttg gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga
    61801 ccgtgatatt gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac
    61861 agtcttattc cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag
    61921 cggtatctat gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca
    61981 tttggatcaa ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa
    62041 tatggtctta ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc
    62101 catttaacag catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc
    62161 aaataaaaat agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct
    62221 tttgaacacc gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa
    62281 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg
    62341 cttttgaaca ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa
    62401 acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc
    62461 tgattgaaca ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc
    62521 ccttttttca cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt
    62581 aactatatgg tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc
    62641 acactttttt aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa
    62701 tattttgaac ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga
    62761 taatcaaaat ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac
    62821 ataaataact aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg
    62881 gtttgttcaa taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt
    62941 cttccgctcc tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca
    63001 tcttgatagc cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta
    63061 agccgacgat tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat
    63121 aatcatttaa tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt
    63181 cgccatattt ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg
    63241 taaagacgac tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata
    63301 aacgttctga taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga
    63361 tcacatcacc aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga
    63421 taaacccttt tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg
    63481 gtacacgtcc accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata
    63541 gcaccgtata ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt
    63601 ttattccagc ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc
    63661 taagtggtcg ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac
    63721 ctgaaagttc agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg
    63781 ttaattcaaa aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac
    63841 ttgcacggcc tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt
    63901 cgatcgtttg actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat
    63961 ctaatcctct ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta
    64021 gaccggcgaa atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt
    64081 caattgtttc caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt
    64141 tgacacatgt tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc
    64201 ctgctctgtt tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata
    64261 tttgcatttt gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt
    64321 ttctacagaa aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca
    64381 gggatatatc agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc
    64441 tgtgcgactg gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct
    64501 tcttcattat gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat
    64561 tcgatttcat ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc
    64621 tcatcataag ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg
    64681 aataattgtt cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt
    64741 gctactttcc ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc
    64801 atataactgt ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat
    64861 ttcattcttc cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt
    64921 tcgatcattg acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca
    64981 gttaaactgt ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa
    65041 gattgagaac atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat
    65101 cgaccagaat aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta
    65161 tttttacgaa tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc
    65221 aacccttctt ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat
    65281 aaatggatcg gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa
    65341 acaatcactg cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct
    65401 tcatttcctt catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt
    65461 gcaaaagcaa ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa
    65521 ccatatgcat tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac
    65581 ttttccagtg ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc
    65641 atttctttca tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat
    65701 ctaattcacg gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt
    65761 cttgatccca gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt
    65821 ttacgaattt ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct
    65881 gataaagttc attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc
    65941 catgactatc ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa
    66001 gacctctttc acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca
    66061 gtggacgttt ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat
    66121 cgagtttttc ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc
    66181 caactgcgcc ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag
    66241 catcataaat atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg
    66301 tatcaccaac tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta
    66361 catttttcat tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat
    66421 acttgtgtgc taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac
    66481 gcaatccaaa ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt
    66541 ctgcttgttc aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca
    66601 acttcataat agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga
    66661 tttttgaaaa ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata
    66721 aaaagagatt gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa
    66781 attcagctac acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga
    66841 acattcaatg tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt
    66901 tctccgccaa gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg
    66961 attggacgtg caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat
    67021 ggtgtgattt tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg
    67081 atttggacga ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg
    67141 attcgattca agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc
    67201 atggctttgt gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga
    67261 atttgtgtcg ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt
    67321 ctacctttag aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg
    67381 tctggatgtg ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt
    67441 ttttctgtca actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat
    67501 cggcttgtac tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca
    67561 cttccaaaca tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt
    67621 ccaaggaaca tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg
    67681 cggatcgcac gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga
    67741 agtccttcca gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca
    67801 ccagtccact ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct
    67861 tgctcctgtt ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct
    67921 tgtctgcgta attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct
    67981 tgtactagtt catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga
    68041 tccaaacgga ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga
    68101 cgagaagtga tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc
    68161 tccactccat ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct
    68221 tcaggagttg gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt
    68281 tcgatatatt tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga
    68341 gctaacgccg gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca
    68401 attaaggtat gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg
    68461 atgacctttt tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat
    68521 cccatatcaa gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg
    68581 attttttgag ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt
    68641 acagggttat tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa
    68701 cgaccaacaa ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat
    68761 gaatctagcg taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta
    68821 cgacgattca ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact
    68881 tgacgggctt ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt
    68941 tcatctttta ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc
    69001 ttcacttcgc tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag
    69061 actcctttag ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa
    69121 atttccgttt cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg
    69181 accactaagg ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct
    69241 tcttcttgtg caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa
    69301 tctcacttcc catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt
    69361 gtatgatctt catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag
    69421 tttccttctt tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca
    69481 tttttctcac tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta
    69541 tctgaaatcc ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact
    69601 agataaccac gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca
    69661 cagttaaaga ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga
    69721 attttcttta tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt
    69781 ccctttgact aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg
    69841 ctttttgtta aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt
    69901 atc
//
LOCUS       DAZKOM010000005        66568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000005 DAZKOM010000000
VERSION     DAZKOM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.005"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..891)
                     /locus_tag="ACG8NF_000313"
     CDS             complement(67..891)
                     /locus_tag="ACG8NF_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0470829.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(864..1544)
                     /locus_tag="ACG8NF_000314"
     CDS             complement(864..1544)
                     /locus_tag="ACG8NF_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470830.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1567..2505)
                     /locus_tag="ACG8NF_000315"
     CDS             complement(1567..2505)
                     /locus_tag="ACG8NF_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0470831.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2750..3601)
                     /gene="ispE"
                     /locus_tag="ACG8NF_000316"
     CDS             complement(2750..3601)
                     /gene="ispE"
                     /locus_tag="ACG8NF_000316"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0470832.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3755..6328)
                     /gene="mprF"
                     /locus_tag="ACG8NF_000317"
     CDS             complement(3755..6328)
                     /gene="mprF"
                     /locus_tag="ACG8NF_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0470833.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6469..7275
                     /gene="proC"
                     /locus_tag="ACG8NF_000318"
     CDS             6469..7275
                     /gene="proC"
                     /locus_tag="ACG8NF_000318"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0470834.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7354..8910)
                     /gene="rny"
                     /locus_tag="ACG8NF_000319"
     CDS             complement(7354..8910)
                     /gene="rny"
                     /locus_tag="ACG8NF_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0470835.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9321..10370)
                     /gene="recA"
                     /locus_tag="ACG8NF_000320"
     CDS             complement(9321..10370)
                     /gene="recA"
                     /locus_tag="ACG8NF_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0470836.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10479..11720)
                     /locus_tag="ACG8NF_000321"
     CDS             complement(10479..11720)
                     /locus_tag="ACG8NF_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0470837.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11960..12538)
                     /gene="pgsA"
                     /locus_tag="ACG8NF_000322"
     CDS             complement(11960..12538)
                     /gene="pgsA"
                     /locus_tag="ACG8NF_000322"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0470838.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12747..13670)
                     /locus_tag="ACG8NF_000323"
     CDS             complement(12747..13670)
                     /locus_tag="ACG8NF_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0470839.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14409..15695)
                     /locus_tag="ACG8NF_000324"
     CDS             complement(14409..15695)
                     /locus_tag="ACG8NF_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0470840.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15688..16956)
                     /locus_tag="ACG8NF_000325"
     CDS             complement(15688..16956)
                     /locus_tag="ACG8NF_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0470841.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17208..17669
                     /gene="mscL"
                     /locus_tag="ACG8NF_000326"
     CDS             17208..17669
                     /gene="mscL"
                     /locus_tag="ACG8NF_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0470842.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17728..18174)
                     /locus_tag="ACG8NF_000327"
     CDS             complement(17728..18174)
                     /locus_tag="ACG8NF_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0470843.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18267..18512)
                     /locus_tag="ACG8NF_000328"
     CDS             complement(18267..18512)
                     /locus_tag="ACG8NF_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0470844.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18627..19205)
                     /locus_tag="ACG8NF_000329"
     CDS             complement(18627..19205)
                     /locus_tag="ACG8NF_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0470845.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19282..19812)
                     /locus_tag="ACG8NF_000330"
     CDS             complement(19282..19812)
                     /locus_tag="ACG8NF_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0470846.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19816..20676)
                     /gene="rlmB"
                     /locus_tag="ACG8NF_000331"
     CDS             complement(19816..20676)
                     /gene="rlmB"
                     /locus_tag="ACG8NF_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0470847.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20669..21064)
                     /locus_tag="ACG8NF_000332"
     CDS             complement(20669..21064)
                     /locus_tag="ACG8NF_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0470848.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21061..22473)
                     /gene="cysS"
                     /locus_tag="ACG8NF_000333"
     CDS             complement(21061..22473)
                     /gene="cysS"
                     /locus_tag="ACG8NF_000333"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0470849.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22470..23006)
                     /gene="epsC"
                     /locus_tag="ACG8NF_000334"
     CDS             complement(22470..23006)
                     /gene="epsC"
                     /locus_tag="ACG8NF_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0470850.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23092..23331)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23328..24788)
                     /gene="gltX"
                     /locus_tag="ACG8NF_000335"
     CDS             complement(23328..24788)
                     /gene="gltX"
                     /locus_tag="ACG8NF_000335"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0470851.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24958..26115)
                     /locus_tag="ACG8NF_000336"
     CDS             complement(24958..26115)
                     /locus_tag="ACG8NF_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0470852.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26149..27519)
                     /gene="radA"
                     /locus_tag="ACG8NF_000337"
     CDS             complement(26149..27519)
                     /gene="radA"
                     /locus_tag="ACG8NF_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0470853.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27582..28109)
                     /locus_tag="ACG8NF_000338"
     CDS             complement(27582..28109)
                     /locus_tag="ACG8NF_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0470854.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28339..29145
                     /gene="proB"
                     /locus_tag="ACG8NF_000339"
     CDS             28339..29145
                     /gene="proB"
                     /locus_tag="ACG8NF_000339"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0470855.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29142..30389
                     /locus_tag="ACG8NF_000340"
     CDS             29142..30389
                     /locus_tag="ACG8NF_000340"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0470856.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30465..30809
                     /locus_tag="ACG8NF_000341"
     CDS             30465..30809
                     /locus_tag="ACG8NF_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470857.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30926..31252)
                     /locus_tag="ACG8NF_000342"
     CDS             complement(30926..31252)
                     /locus_tag="ACG8NF_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470858.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31867..33069)
                     /locus_tag="ACG8NF_000343"
     CDS             complement(31867..33069)
                     /locus_tag="ACG8NF_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0470859.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33186..33701
                     /locus_tag="ACG8NF_000344"
     CDS             33186..33701
                     /locus_tag="ACG8NF_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0470860.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33900..34346)
                     /locus_tag="ACG8NF_000345"
     CDS             complement(33900..34346)
                     /locus_tag="ACG8NF_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0470861.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34393..35871)
                     /gene="msr(C)"
                     /locus_tag="ACG8NF_000346"
     CDS             complement(34393..35871)
                     /gene="msr(C)"
                     /locus_tag="ACG8NF_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0470862.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36225..36854)
                     /locus_tag="ACG8NF_000347"
     CDS             complement(36225..36854)
                     /locus_tag="ACG8NF_000347"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0470863.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36838..37302)
                     /locus_tag="ACG8NF_000348"
     CDS             complement(36838..37302)
                     /locus_tag="ACG8NF_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0470864.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37789..39057)
                     /locus_tag="ACG8NF_000349"
     CDS             complement(37789..39057)
                     /locus_tag="ACG8NF_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0470865.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39288..40025)
                     /locus_tag="ACG8NF_000350"
     CDS             complement(39288..40025)
                     /locus_tag="ACG8NF_000350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0470866.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40092..40661)
                     /locus_tag="ACG8NF_000351"
     CDS             complement(40092..40661)
                     /locus_tag="ACG8NF_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0470867.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40789..41661
                     /locus_tag="ACG8NF_000352"
     CDS             40789..41661
                     /locus_tag="ACG8NF_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0470868.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            42003..42875
                     /locus_tag="ACG8NF_000353"
     CDS             42003..42875
                     /locus_tag="ACG8NF_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0470869.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42872..44830
                     /gene="nagE"
                     /locus_tag="ACG8NF_000354"
     CDS             42872..44830
                     /gene="nagE"
                     /locus_tag="ACG8NF_000354"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0470870.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45162..46013)
                     /locus_tag="ACG8NF_000355"
     CDS             complement(45162..46013)
                     /locus_tag="ACG8NF_000355"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0470871.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46136..46603
                     /locus_tag="ACG8NF_000356"
     CDS             46136..46603
                     /locus_tag="ACG8NF_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0470872.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46866..47348)
                     /locus_tag="ACG8NF_000357"
     CDS             complement(46866..47348)
                     /locus_tag="ACG8NF_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0470873.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47448..48311)
                     /locus_tag="ACG8NF_000358"
     CDS             complement(47448..48311)
                     /locus_tag="ACG8NF_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0470874.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            48909..49784
                     /locus_tag="ACG8NF_000359"
     CDS             48909..49784
                     /locus_tag="ACG8NF_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0470875.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(49929..50081)
                     /locus_tag="ACG8NF_000360"
                     /pseudo
     CDS             complement(49929..50081)
                     /locus_tag="ACG8NF_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(50349..51680)
                     /locus_tag="ACG8NF_000361"
     CDS             complement(50349..51680)
                     /locus_tag="ACG8NF_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0470876.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(51829..52542)
                     /locus_tag="ACG8NF_000362"
     CDS             complement(51829..52542)
                     /locus_tag="ACG8NF_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0470877.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            52717..54141
                     /locus_tag="ACG8NF_000363"
     CDS             52717..54141
                     /locus_tag="ACG8NF_000363"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0470878.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            54440..55147
                     /locus_tag="ACG8NF_000364"
     CDS             54440..55147
                     /locus_tag="ACG8NF_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0470879.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            55134..55457
                     /locus_tag="ACG8NF_000365"
     CDS             55134..55457
                     /locus_tag="ACG8NF_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0470880.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(55546..56583)
                     /locus_tag="ACG8NF_000366"
     CDS             complement(55546..56583)
                     /locus_tag="ACG8NF_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0470881.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(56904..57887)
                     /locus_tag="ACG8NF_000367"
     CDS             complement(56904..57887)
                     /locus_tag="ACG8NF_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0470882.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(58302..59072)
                     /locus_tag="ACG8NF_000368"
     CDS             complement(58302..59072)
                     /locus_tag="ACG8NF_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0470883.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(59065..60000)
                     /locus_tag="ACG8NF_000369"
     CDS             complement(59065..60000)
                     /locus_tag="ACG8NF_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470884.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(60316..61965)
                     /locus_tag="ACG8NF_000370"
     CDS             complement(60316..61965)
                     /locus_tag="ACG8NF_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0470885.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(62082..64685)
                     /locus_tag="ACG8NF_000371"
     CDS             complement(62082..64685)
                     /locus_tag="ACG8NF_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0470886.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(64697..65746)
                     /locus_tag="ACG8NF_000372"
     CDS             complement(64697..65746)
                     /locus_tag="ACG8NF_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0470887.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(65772..66086)
                     /locus_tag="ACG8NF_000373"
     CDS             complement(65772..66086)
                     /locus_tag="ACG8NF_000373"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0470888.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(66102..66548)
                     /locus_tag="ACG8NF_000374"
     CDS             complement(66102..66548)
                     /locus_tag="ACG8NF_000374"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0470889.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT        19388 a        14045 c        11650 g        21485 t
ORIGIN      
        1 tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt
       61 tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat
      121 caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa
      181 cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac
      241 gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga
      301 tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt
      361 gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa
      421 agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa
      481 gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga
      541 ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa
      601 accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag
      661 atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag
      721 gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat
      781 cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa
      841 gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc
      901 tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg
      961 gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat
     1021 tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg
     1081 tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct
     1141 attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga
     1201 tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact
     1261 aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa
     1321 ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa
     1381 ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca
     1441 gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca
     1501 tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc
     1561 taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata
     1621 attttcgcct gctttgattt gttcattagt caagctttct aaaggattca acacttctag
     1681 cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa
     1741 ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg
     1801 tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt
     1861 cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag
     1921 ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt
     1981 cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac
     2041 ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc
     2101 atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc
     2161 tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac
     2221 aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct
     2281 ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac
     2341 gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac
     2401 gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag
     2461 taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc
     2521 aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa
     2581 cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt
     2641 aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata
     2701 ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg
     2761 ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc
     2821 ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat
     2881 ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg
     2941 ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga
     3001 tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa
     3061 acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca
     3121 tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga
     3181 cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac
     3241 agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc
     3301 cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga
     3361 tattgtattt attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg
     3421 gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata
     3481 gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc
     3541 gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca
     3601 tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag
     3661 tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt
     3721 ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg
     3781 ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa
     3841 atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt
     3901 ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga
     3961 tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag
     4021 ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta
     4081 acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg
     4141 cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga
     4201 caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt
     4261 tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt
     4321 ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa
     4381 gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta
     4441 ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat
     4501 aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc
     4561 gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct
     4621 gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg
     4681 acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac
     4741 ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg
     4801 ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg
     4861 aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat
     4921 agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag
     4981 ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta
     5041 acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta
     5101 aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa
     5161 gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa
     5221 aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga
     5281 ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat
     5341 gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc
     5401 ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc
     5461 gataatataa tagccaaact actgctacat cttgccgaac agacaattga ctcattccta
     5521 agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca
     5581 atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact
     5641 tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca
     5701 gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa
     5761 aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa
     5821 aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca
     5881 aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg
     5941 tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga
     6001 gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg
     6061 gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga
     6121 cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc
     6181 tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa
     6241 agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt
     6301 gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg
     6361 tcatacccca atgttatctt atttatttta atttttccac tttagacctg ttctttgctt
     6421 gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga
     6481 tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca
     6541 gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct
     6601 gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc
     6661 gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat
     6721 agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca
     6781 gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta
     6841 ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa
     6901 gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt
     6961 acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg
     7021 ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa
     7081 gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta
     7141 gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca
     7201 ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg
     7261 ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg
     7321 gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt
     7381 ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt
     7441 acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat
     7501 tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt
     7561 cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc
     7621 accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt
     7681 agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta
     7741 cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc
     7801 tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc
     7861 tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc
     7921 ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg
     7981 agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt
     8041 acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag
     8101 tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac
     8161 tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat
     8221 attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac
     8281 tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa
     8341 gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag
     8401 agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc
     8461 ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac
     8521 gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc
     8581 aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg
     8641 aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct cactttcaac
     8701 ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt
     8761 ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat
     8821 ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc
     8881 gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt
     8941 tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc
     9001 atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa
     9061 ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa
     9121 atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc
     9181 tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa ataactcacg
     9241 atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc
     9301 gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg tcctttttca
     9361 tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact
     9421 tctgccatca tttcaggatg attagccata tagattttag cgttttcccg tccttgacca
     9481 atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact
     9541 gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca
     9601 gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca
     9661 acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg
     9721 tagaatttca atgcgcgtcc tccaggagtg atttctggat tcgcaaacat cacgcctact
     9781 ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag
     9841 agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt
     9901 tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct
     9961 acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc
    10021 tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac
    10081 gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg
    10141 tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca
    10201 cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta
    10261 gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt
    10321 tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc
    10381 ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga
    10441 aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt
    10501 ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca
    10561 aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct
    10621 gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct
    10681 gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt
    10741 ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag
    10801 taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct
    10861 tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt
    10921 agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct
    10981 actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca
    11041 tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt
    11101 ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc
    11161 ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt
    11221 aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg
    11281 atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt
    11341 agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga
    11401 tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac
    11461 ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg
    11521 ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc
    11581 tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt
    11641 tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca
    11701 gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat
    11761 atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt
    11821 tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac
    11881 atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa
    11941 cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt
    12001 aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg
    12061 gaataccgat caggctgaat gggatattat tgataaacag caagataata gcaaccattt
    12121 gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa
    12181 gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac
    12241 caggggcctt tccttggtca accaacatga taaaagcagt cataactagc attttatctg
    12301 ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc
    12361 catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga
    12421 cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca
    12481 aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac
    12541 tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt
    12601 tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt
    12661 aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa
    12721 acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt
    12781 tttggactct gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc
    12841 gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg
    12901 gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc
    12961 acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct
    13021 gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt
    13081 tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc
    13141 tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg
    13201 attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc
    13261 aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat
    13321 tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg
    13381 accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct
    13441 cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg
    13501 tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt
    13561 aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt
    13621 tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc
    13681 aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg
    13741 aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa
    13801 gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc
    13861 tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat
    13921 aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    13981 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
    14041 acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    14101 caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac
    14161 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    14221 tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag
    14281 catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct
    14341 gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa
    14401 atactggttt attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct
    14461 tccccagctg ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat
    14521 aaagttcgat cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt
    14581 aatgattgga aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac
    14641 acttccggat catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct
    14701 ggttcgtccg tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca
    14761 aaaccaaagc tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta
    14821 ttgcctaaaa tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt
    14881 ccttcttgcg gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg
    14941 gctgcttcca atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca
    15001 atttcttgct ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat
    15061 ttctcaggct ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc
    15121 cgatagcacg tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca
    15181 atatcgatat gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc
    15241 cagttagggt cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt
    15301 acagtttctt ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt
    15361 tctacctgat ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg
    15421 gcggaagctc cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac
    15481 ttatgttcta aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag
    15541 ggaataaatt cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga
    15601 tagtcatttt taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt
    15661 gtttcattga tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa
    15721 gattgcctgc aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc
    15781 catacgtttc aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa
    15841 agaagcttcg attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat
    15901 tgcttttgtc tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg
    15961 ctcgtgaatc agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact
    16021 catgaatcct cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc
    16081 tctgacattc atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat
    16141 tagcgcaaaa cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt
    16201 tgcttgaacg attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt
    16261 ataaaacaga tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc
    16321 agctgctttg tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat
    16381 catttcttga tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac
    16441 gcccaaccct gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata
    16501 ggaagcaaaa gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt
    16561 ttccaagtca aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac
    16621 ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt
    16681 cacaaaactg atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc
    16741 aaagcttgcg ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt
    16801 caaactattt gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg
    16861 cagacgcgca gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac
    16921 atgaagattc acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt
    16981 gattgcccgc ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga
    17041 aatttataag ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag
    17101 catagatgtt ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt
    17161 ttatttttcg atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat
    17221 tcaaagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta
    17281 gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt
    17341 ggtttgtcgc aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact
    17401 ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat
    17461 ttctgatcac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt
    17521 tacgctccaa agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta
    17581 gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc
    17641 aaacaaatct ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt
    17701 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
    17761 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
    17821 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
    17881 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
    17941 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
    18001 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc
    18061 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
    18121 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
    18181 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc
    18241 ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact
    18301 gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga
    18361 tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt
    18421 tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa
    18481 tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat caactatacc
    18541 acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat aaaaataaat
    18601 aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt
    18661 attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat
    18721 tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt
    18781 tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaattgct
    18841 aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg
    18901 tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga
    18961 gaatagattt gattgcttat aacgctttta taataacttc caaagctaac acctttgctt
    19021 ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca
    19081 gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt
    19141 tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca
    19201 gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat
    19261 cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga
    19321 caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat
    19381 atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata
    19441 agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt
    19501 cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact
    19561 gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg
    19621 ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt
    19681 cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt
    19741 tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag
    19801 ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc
    19861 agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc
    19921 atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc
    19981 aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac
    20041 ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac
    20101 agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc
    20161 tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga
    20221 cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt
    20281 ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag
    20341 aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca
    20401 tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg
    20461 tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc
    20521 atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg
    20581 atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg
    20641 tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac
    20701 accagcggat caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga
    20761 gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat
    20821 ggcttttagc atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt
    20881 cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg
    20941 cacgatgcag cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga
    21001 tttcgtaaat cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc
    21061 tcatgcttct cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc
    21121 tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt
    21181 tcgttcttcg atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat
    21241 accgaagatc gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga
    21301 aactttcggc tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat
    21361 cccatttgct gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc
    21421 taacaaagcg attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa
    21481 tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt
    21541 cgtttcactg tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac
    21601 ttgtggatca acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact
    21661 cattttttca tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa
    21721 ttttttccct gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa
    21781 atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga
    21841 acattcaata tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc
    21901 aggttttgct tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc
    21961 ccctgtacgc tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc
    22021 ataattttca aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc
    22081 tttttcaacc aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg
    22141 atgcagtgta gcaggttcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa
    22201 acgatcagca acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc
    22261 gtctacatcc gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg
    22321 tcgaattgta tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac
    22381 agtcggtccg cagacataca taccgacatg tcccttttgt atagggatga attcttcttt
    22441 ttttctggtc aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg
    22501 attcgaacga cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac
    22561 acaacggcac ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc
    22621 gcattcgcag agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt
    22681 cctgtgccgc ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt
    22741 tcgccaatga cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct
    22801 ggatggatct caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa
    22861 aagagccggt gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca
    22921 gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca
    22981 tcaatcgctc gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag
    23041 gaaatcatct tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac
    23101 gctttccgcg tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct
    23161 gttatacgtt cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc
    23221 gccaagacca ctcccagagg catttcggca accattcatt gataccttgc accacccggt
    23281 atctctcttg taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact
    23341 tgatttaaat gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct
    23401 ggaccatgca tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt
    23461 acgccagttt ctttttgaac agctttgatg gcagctttga cagttggagc atctactact
    23521 tccatttgtt ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca
    23581 agtacttctt ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga
    23641 tcaacgattt ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc
    23701 cattcgattg tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc
    23761 agacacatgt cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat
    23821 tgcagttttt tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt
    23881 ttgatcaatt cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat
    23941 gcaataaagt tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat
    24001 tgcaagattg attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat
    24061 gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt
    24121 ggtgtattgg caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca
    24181 tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc gtttttggat gacaaagtcg
    24241 ccaccgatat tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat
    24301 tctgtattgc gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct
    24361 gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt
    24421 gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct
    24481 cgattgctag caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga
    24541 cggtaagggc cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa
    24601 gcaaggtttt ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc
    24661 tcaatacgaa taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca
    24721 gttcttgcat ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact
    24781 ttcgtcattt tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca
    24841 gttacggctt ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta
    24901 aggtaaatag taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta
    24961 ttttgattgt ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg
    25021 tgcaggttta gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac
    25081 ttcgatacgt tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc
    25141 aaggtaggct actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt
    25201 ttcaccaggg atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg
    25261 tacattttgg aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc
    25321 taaaagctta gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc
    25381 atcgtacatt tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc
    25441 gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta
    25501 aagaacaaaa ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata
    25561 aatccgaccg tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc
    25621 tgattttcgt tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt
    25681 gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc
    25741 cagatagcct aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc
    25801 catattgtaa agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat
    25861 cgcaccgaag agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat
    25921 aatagctgag atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa
    25981 caggctgttg gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca
    26041 agctgagggt aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat
    26101 gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac
    26161 acgcttcaac gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc
    26221 ccagttacct aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc
    26281 tttgactcgt tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc
    26341 gatgaaacat tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc
    26401 gatagctaaa tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc
    26461 atcttgattc tgaagaagca acccagctct tttttccagc actgccatga tcagagatac
    26521 ccgattgaaa tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac
    26581 taatgcttgg atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga
    26641 gccagttgca ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc
    26701 caatccatgc tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt
    26761 tactgctcgc aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc
    26821 caccatatgt tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac
    26881 gataaaaatg gctatcccat ttgttttagc ggttttgagt agttctgcag tggtttctct
    26941 tacttggctg acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga
    27001 atcaatgata acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc
    27061 tgtttcagcg tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt
    27121 tatctgctct gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa
    27181 ttgctgagaa acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa
    27241 gaccatagac cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt
    27301 tttgatccga ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc
    27361 tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat
    27421 cgtattccat tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca
    27481 attttggcaa acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct
    27541 aatcatgaga cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca
    27601 aatccgcctt gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag
    27661 atcccttggc cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg
    27721 aattggaaca tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca
    27781 cctactccgt ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc
    27841 tgcaaaaatt catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc
    27901 aattgttttt cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc
    27961 tttttccagc ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca
    28021 tagccagcag catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta
    28081 gaaataactt caaatcctct ttttctcacc ggttttcctc cctaaataaa ctactcctat
    28141 tctaacatat ccgaggctgt aaaagacttt ctttacaaat tcttcaagaa attctgctat
    28201 cttaaagtaa accattaatt cgttcttttt ccaaacgact gcaagttgta caaaaaaact
    28261 attttttaca tgactttact cgtcaaaaca agtagaatag aacgaaatta atgagtggat
    28321 atttgattga ggtgtcacat gcgtaaagaa attaaaaatg caaaacgaat tattgtcaaa
    28381 gtaggaacaa gtacattgat ttatcccaat ggaaatatca atcttgcggc aatcgatcag
    28441 ttagcttttg tcctttctga tcttagaaat cggggaaagg aagtgatctt agtctcatca
    28501 ggagctgtag gcgttggtat gcataaatta catttgtcag aacgtccgac tttgattcct
    28561 gaacagcaag ctgcggcggc tgttggtcaa gccgaactga tgaatatcta taatcaaaga
    28621 ttcagtgtat ataatcaaca gacggcacag atcctcgtga caagagacgt ggtagattat
    28681 cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc ttttgattat ggacacgatc
    28741 cctatcgtga acgaaaacga tactgtagca gtggatgaat tagatcatct gacaaaattt
    28801 ggcgataatg atcagctgtc agccatcatg gcaaaacttg tcgcggcaga ccttttgatc
    28861 gtcctttctg atatcgacgg gttttattca gataatccga ctacaaatcc cgaggctcag
    28921 atgtatcgct atatcgataa aatcgatgaa aagattctgg cacaagcagg tggtgctggt
    28981 agcacatttg gaacaggcgg tatgcagagt aaattaaaag ccgcttctag gatatttgaa
    29041 gcaaacagcg caatgatttt agcaaatgga aaaaatccgg caattatttt tgatattctt
    29101 gcaggaaaag aagtaggtac tttatttaag gaggacttga aatgactgac ttgatgcaat
    29161 taggacaaca ggcaaaagat agcgcgcaac aactagcact tatgcctaca aaccgcaaaa
    29221 atgagctact tgttcaaatg gcacaaacca tcaaaaagaa tcaacaagcg atcattgaag
    29281 caaatcataa agacttagaa aaagcagtcg aaaacaatat cagtgaaacg atgcaagacc
    29341 gcttgcagct gacacctgag cgaatcaatg ccatggctac agagatcgaa aaaattgctt
    29401 cgttagacga tcctattgga aaagtagatg agatgtggac gaacaccgac ggattgagaa
    29461 tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat ttacgaatct cgtccaaatg
    29521 tcacgacaga tgcagctagt ctagcattca aatcagggaa tgccgttatc cttcgcggtg
    29581 gcaaagaagc attcttctcg aatcaattat tagtccaatt attgcagcaa gtactggtca
    29641 gtgctggcga atctccttat gccatccagt ttgtcgatga tacttctcat gaaactgcag
    29701 gacagttgat gcgattgaca gaatacttag atgtgctgat tcctcgaggc ggagcaaaat
    29761 taatccaacg agtcaaagaa caggcaacgg tacctatcat tgaaacaggt acaggaaata
    29821 atcacgtata tatcgataaa gaagcacagt tatctatggc agtgaacatc atcgtcaatg
    29881 caaaagcttc ccgtccatct gtctgtaacg cggcagaaac attgctgatc cattctgaga
    29941 tcgcaccttt tttcttgcca gctattgaaa aagaactggt agaacacggg gtctctttac
    30001 gtgcggatgc tcgtgcgtta gaatatctag agacggctgc tttagcagaa gacacagatt
    30061 gggatactga atatctagat tatatccttg ctgtcaaagt ggtcgattct ttggatgaag
    30121 caattgcaca tatcaatcgg cataatacta aacattcaga aacgatcgtg acagatagtt
    30181 atgcggctag tcaacgtttc ttaaatgaag tggacgcggc ggctgtctat gtcaatgcct
    30241 ccactcgttt tacagacgga tcggtatttg gttttggtgc agagattggt atctctactc
    30301 aaaagttgca tgcccgtggt ccaatggggt taaatgaatt aacttccaca aaatatatca
    30361 tttatggcga tggacaaatc cgcgaataat gtttcgtgtg aaacattatt cccgcctttt
    30421 gccttttttc tgtaaaggtt ctatccaact agaaaggtgt caatatgttt gaaattttaa
    30481 tgattttttt tatttacctt atttctctga atatcgctgc ttttctggga gtcggtattt
    30541 tatccctttt ctttcaattc aaaaaacgaa gcatagggag tcaacgggaa aaatggtcgc
    30601 agtattttga caaaatcggg caaaaagggc tggttacacg cttgcacatt agttacatgg
    30661 ttgcattatg cttattagcg acaatcaatt actattcttt tttcgatcat tcaatcgctt
    30721 atacgattac gctactgatt gctgggattt ttcacttatc ttataaatat cagctgaata
    30781 aaaaccactt gaatcggact ttccgctaaa aaatatccgt acgataagta gagtgccttg
    30841 aagatcggca aaaaactgcc ggacttttta gaggctgtga caaagatttt gtcacagcct
    30901 ctttttccat ataaacggcg ttcgcctacc agctgcctgg taaatctgtt tcctcttcgt
    30961 taagaagcga aaatccgtca aatgtttcgc catcataata acgaagcagc tcatccatgg
    31021 acaagttcat cgttttttct ttttcttctt ccgctgattc agccagtagc tgttcccgat
    31081 acacctgata cataccagct attttttgct gcatcgcttt tctagtttca ccttccgcat
    31141 tgattattgg caattctttg atatagccac gataaatcaa ccgatcatct gaaaattcaa
    31201 tgggtaattc aacgagtgtc aacccgactt cctgaaaata ttcttccagc atctattatc
    31261 cccccattct ttttcttaaa tgaattttag cttacctact gaaaattttc cagtttcatt
    31321 attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac atcccctttt tccgaataaa
    31381 tggtgttctt ctgccaactc ctcaatcata agtcacaata ttcaacaaac atgagaaact
    31441 gttttttgaa attgttcctt gtgattcaga gttaaacgac agtctcacaa cctcttggta
    31501 aacggcgttc aatcagctga ccctcggtat tagctcaaaa aacggtaaaa tatgagaagc
    31561 tatttttccg ttttgtttct ctaatactta ggataaaacg ctgattttac aacctctgtt
    31621 aaacggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa atatgaggaa
    31681 ctatttttcc gtttgtttct ctaatactca ggacaaaacg ctgattttac aacctcctga
    31741 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag
    31801 aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct
    31861 gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga tacaacatac
    31921 actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc
    31981 aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa aactatagct
    32041 ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg tcaacagtgt
    32101 attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa ggactggcat
    32161 agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg ctgctaaaat
    32221 tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg aagcaatagc
    32281 cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat agccaatcaa
    32341 taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca atgcgattag
    32401 tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa ggcctacttt
    32461 tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac cgcaaaacat
    32521 caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga ccccaccgac
    32581 tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga ttgctttttc
    32641 tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa ataaagcatt
    32701 cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag ataatccgcc
    32761 aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt tcaaaccgat
    32821 ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca tgtaactagt
    32881 aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg tttttaagat
    32941 tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta caactagcgc
    33001 tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt gattctccaa
    33061 tatttccatc cctttctctc tattagcatc atacatgatt tattttacat gtcaatatgc
    33121 acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg agaggtagga
    33181 tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa ccacgttctg
    33241 ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt cagatctata
    33301 caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa gacgagcact
    33361 ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag gaatggatca
    33421 aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc tatgcttttt
    33481 cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa cagctgaaca
    33541 aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag aaacaagaat
    33601 ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt
    33661 gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac agaacctttc
    33721 tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag ttggaaaaga
    33781 aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt tctagaactg
    33841 cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac agtgttccat
    33901 cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac gatgatcaac
    33961 tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc agagaataat
    34021 tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt caccattcct
    34081 aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg atttactggt
    34141 agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa aatgaaagcc
    34201 accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc ttgatgcagc
    34261 cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt
    34321 attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt cacgaaaatc
    34381 atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc cttttatttc
    34441 ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt
    34501 tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat caataaaatt
    34561 agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag caatcgtcag
    34621 acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa
    34681 ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt gtctcatgac
    34741 aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat atacttgaaa
    34801 gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt ctaataaggt
    34861 tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag aaaactgaaa
    34921 atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt gtcccatgat
    34981 gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt gtttcatctg
    35041 ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt tcgcattctt
    35101 ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat ccagtctgat
    35161 ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt ttttcttttc
    35221 ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt ctcgtagctg
    35281 agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg aaaactctcg
    35341 gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa aatagcgatc
    35401 atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca gtcgatcaat
    35461 actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt cataagaaga
    35521 aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc gctctgaagt
    35581 ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct ttgcttcagc
    35641 gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt tcccatgatc
    35701 gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac cattttctcc
    35761 tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata gttctagctg
    35821 gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca tatgttttac
    35881 actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa aaatctgttt
    35941 gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga ctaaatcacc
    36001 aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca tactccatcc
    36061 atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca aaagttcgaa
    36121 acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt taccacacac
    36181 gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc gaacatagtg
    36241 atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt tcctattgat
    36301 aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc caactgatct
    36361 gcttcgaatc caccaattgg atttgtttca tagccatgat cacgcgcaac taacatgaat
    36421 tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc tcttgaaaaa
    36481 ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt tggcatcttc
    36541 cctgattcgt aagcttggtt gtagatttct tcgccatatt cgtaacattg catatcgcca
    36601 aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg gatcaaagga
    36661 cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg tttcatattg
    36721 acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc gtgtgggatt
    36781 ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt tgaaaaatca
    36841 tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc aaagttgggt
    36901 attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga aagccgggga
    36961 tagcacggaa cctgtatctt tatggtccat ttcacaatgc ttgattttct tctgggcttt
    37021 ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac gcgaaacata
    37081 gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa tctgcaactt
    37141 ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca tgatctcgta
    37201 tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt gatcggctaa
    37261 tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct cctttctaat
    37321 aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga
    37381 atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa gaaatttttt
    37441 gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga ttattgattc
    37501 ttatcaaact atagaagata gctcttctgc agtagataat tttacaccgc caaaaatttc
    37561 cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt atgcttaaac
    37621 agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa
    37681 aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt
    37741 tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt aataaccata
    37801 aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt ccttagcact
    37861 caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat aaattggctt
    37921 ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg ttaaagagtt
    37981 gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt ataaacggta
    38041 gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc agcctacagt
    38101 tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg tatgacggtt
    38161 atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg tcagattata
    38221 ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat aacgacctgt
    38281 tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac cggaatagta
    38341 gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt gcaatgattc
    38401 tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc cattcacata
    38461 ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc caaatagatt
    38521 gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga tggcttgagc
    38581 aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag ttgaggcaat
    38641 actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag catagccagt
    38701 atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt gcccatctgt
    38761 caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg ctgatgtact
    38821 gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg aaattgtatt
    38881 ttctgagaca gtagctgact gcaaagtact ttctgtatcg ttttcaattc ctgtttctga
    38941 agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca ctgttgtgtt
    39001 ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg ctttcatctt
    39061 ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa aagtaaaagc
    39121 aagtcaatgt ccatatttaa ataacatttg tttcttttta ataaatttgc tttttctgca
    39181 taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga tggtcgtttg
    39241 aataactatt taacaagtaa ttctctctgg atattatccg ttatggatta tacttcctgt
    39301 gtcgtcggac ggataatcat gttgctgaca gacatccggt ctggcgtttc tatcgcaaat
    39361 gctacagcag aagctatatc ttcagatgtt aatccccatt ctttttgcgc ttcatgaagt
    39421 tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac agctcctgga
    39481 gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc ttccatgatt
    39541 gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac aacatgtccc
    39601 gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat tggaagtacg
    39661 gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg ccattcatca
    39721 cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta caatacatcg
    39781 attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc aaaatctctt
    39841 acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat tgcttttagg
    39901 cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa tagcttcgtt
    39961 gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc tgataaatca
    40021 ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta tctcttattt
    40081 catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg gattcctgca
    40141 acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac aatggcattc
    40201 tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc atttttcttg
    40261 atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag atggttcacc
    40321 gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat gccacctaag
    40381 tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat atgtttgccg
    40441 aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc actaatgata
    40501 ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag cgctgctaaa
    40561 ggctcttcgt tttccgcttg gatctgatgg atcacttcaa agagcggatc cgttactcga
    40621 atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc tccttttatc
    40681 cttactttat aacggacggc ttatgatgtc taatacttat aaaacatact taactattcc
    40741 ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat ggatattcgt
    40801 atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc agcaaaatct
    40861 ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga agaacttggt
    40921 gtgacacttt tcactcgagg ctcccgagag atacagttga cggattctgg tcgatatctt
    40981 tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat
    41041 gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc aatctctttc
    41101 atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt tcatttatat
    41161 agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga ttttggactc
    41221 gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc ccaagatcgt
    41281 tggggacttt tgatacatcg gaatcatcct ttagcaaagc aaacagtcat ccatccgcag
    41341 gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag tcaactagct
    41401 gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa cttattgtac
    41461 aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga tgggatcatc
    41521 aatacttatg ggacggaaaa agtattcatc ccactcgatc cgccattaca agcttcactg
    41581 aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc
    41641 ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt tttaacaacg
    41701 aataagttaa gcaaaagatc caatgattaa ataacataag tttcttaatt tgttccttcc
    41761 ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt gacaaaacat
    41821 aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac tagatcatac
    41881 taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt gtctattttt
    41941 gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc
    42001 gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac ggtggacagg
    42061 aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat ttgatttttg
    42121 atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt
    42181 tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt gatcatgcgg
    42241 aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc gaccacattg
    42301 ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt ttaagagaaa
    42361 tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag tatctgacaa
    42421 aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc catattcaca
    42481 gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg attgtatctg
    42541 acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa ccattttcac
    42601 tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc catgcgcaac
    42661 aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct
    42721 ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga
    42781 aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca atcgtacaaa
    42841 caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac gaatgggacg
    42901 ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag ggatcggaaa
    42961 ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg gcggtaacgc
    43021 agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga tgtcagtgga
    43081 caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag taaatgtttt
    43141 aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg atccatcttt
    43201 tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg cagctcttta
    43261 taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg gcaagcgtct
    43321 agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg
    43381 gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt taggctgggg
    43441 tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat tgcatcaagc
    43501 aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta aatttttggc
    43561 ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc caatcatgat
    43621 gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag aaaacaaagc
    43681 aaaaacagct tccttgatgt tagcagcagc ttttgcttca ttctttactg gtgtaacaga
    43741 accacttgaa ttctcattta tgttcgttgc ttggccatta tatgtcttgc atgcaatttt
    43801 cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat ttgcctttag
    43861 tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc agccattgat
    43921 gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta actttgctat
    43981 caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag atgacgatac
    44041 tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct acgctgcatt
    44101 aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc gtttgcaagt
    44161 caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac ctggtatgaa
    44221 gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga
    44281 tgagatggca aaactacatg gtggtgctgc aacacgacct gcacaaacaa acaccgtggt
    44341 taaaacagaa acatttgccg gtggaacgat cgaacaagat atttacgcta tcgcaaatgg
    44401 gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga
    44461 cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg gtacgatcac
    44521 aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta tcgaagtctt
    44581 gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt tatatgtaaa
    44641 agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg ccgctattca
    44701 atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca aagtccaaag
    44761 cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa
    44821 caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca ggagctgaga
    44881 caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg ccaactccgc
    44941 ggtaataagt ccagccaaac gtaacatatg aagagctatt ttttatttga ctcttcttat
    45001 tacttgatgc agaacagctt ttttacaacc tctgataaac ggtgttcaaa aaccagttta
    45061 tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttcttc
    45121 atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg atccaatatt
    45181 tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc taaatcatca
    45241 gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa catgtcagct
    45301 gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc acttgcttca
    45361 ataatttcta actttttacc tgtcgcacgc tctaatgcct tggctaattc ttcatagtca
    45421 gttagattgc cggataattc taagatttct ggagagtcac tttctactag tacttttgct
    45481 gcagcttctg catattcacg tttcaatacc caacctgttt ttccatttcc tgcagcataa
    45541 acgaattgac cagtttttaa agcctgccca atgataggta attcattctc tacataccaa
    45601 ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc agtgaattgg
    45661 tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt ataagcgata
    45721 taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc ttgacgattt
    45781 cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc tttcattgag
    45841 tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag tgcttctcct
    45901 ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt ttttaagaat
    45961 tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg catatttgcc
    46021 actccctatt ctttttattt gttaagattt acagctgtac atttataaat tacaccttta
    46081 tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac aactgatgaa
    46141 gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa tgtttcaaga
    46201 tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg cagcaaatgt
    46261 acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa ccggaaaacc
    46321 gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct atcgcagcat
    46381 cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt gcgtcgttgg
    46441 tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa aggcagaaga
    46501 agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt tggaaaaaga
    46561 aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc tactgtgcct
    46621 gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg acaaaagcct
    46681 tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca tgacgtcttg
    46741 ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca aaatatggaa
    46801 agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc acgacctcta
    46861 tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt cccgttggta
    46921 actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc tacttctctt
    46981 aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg cgattctgct
    47041 aaagcgatta caacaagata cgttcctttt gggaatggga tgattcgggt agtgtttgga
    47101 acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata gggttcgttg
    47161 actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt tccattaccg
    47221 ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc cgtattgcta
    47281 ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc gatcctatac
    47341 tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa attaaaggga
    47401 aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta tttttttgct
    47461 ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca ttttgtaaaa
    47521 gagatctcgg gagcttcgac taactcataa tctgaagaag gaagccattc tgcatagatt
    47581 ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa aatggcccaa
    47641 gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt tgtcgtagca
    47701 aagccaatca tgtgcatcaa actgcccttt tcttccaagc gggctccatc aaatccataa
    47761 gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc ttccctttga
    47821 gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc aaatttgaca
    47881 ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat acgaaattcc
    47941 atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga tttttgtatg
    48001 ccgtatctgc tgatttctga aggcagatat cctgaccaat cacgaaaagc tcttgaaaaa
    48061 ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga tccatctagg
    48121 agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag cgacatccct
    48181 gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt ttcggctatt
    48241 attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa ttggttcaga
    48301 tctttaagca tatttatctt cctccttgct tgccttttta tcatattatc aaagaaagtc
    48361 aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt atggatctaa
    48421 agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct atctatgttt
    48481 tacctttaga aaaataaatt ttggggaaga cacattctaa gtaaacaaag cggtaaaaat
    48541 atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa ttgttgttca
    48601 aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc tcttttatca
    48661 attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata aataaaaaac
    48721 aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat tctctaagaa
    48781 aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa tatgtaaaat
    48841 cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt tcatgaaagg
    48901 agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa cgcggttaca
    48961 gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac ttgtccgcaa
    49021 cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag accaacatga
    49081 cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct gctgtcttat
    49141 tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa ctctcgtccc
    49201 ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt cttatagaac
    49261 atccgcaatt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata aattattcag
    49321 ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac aaaaacagga
    49381 aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt gaatcgctca
    49441 ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt gctactctcg
    49501 aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag gactgttatt
    49561 atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa atgttctctt
    49621 ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac tccataaaaa
    49681 tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa atagataaaa
    49741 aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta aacgataaca
    49801 accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga atcatgcaaa
    49861 ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta ttaggtacgc
    49921 gttttgtttt ataagtcatg acaaatttcc ttcgttgctg ggtagatttt ccggcattgt
    49981 tgatagattt tatgatactt ttctacattt tgtaagagag gttccactgc atctgtatat
    50041 tggataaaat tctgacaaca actttcaggt gtgtcgaacc actctaatct ttaaagataa
    50101 atattaaaat aacgattaat aatattaaca gtctttattt ttcaccacca aaaacctatc
    50161 tttgcatata ctaaatagac gtatatacac agataggttt gtcttgtgaa aaataatgat
    50221 tatccttacc aaaaaatgtt tgtttttcta catgttcgta cttatcaaaa aatatgatcc
    50281 ttattgaaat tactggctat ttatattcca acgacttctt gtgttaattt ttgttatctc
    50341 atagtaaatt atgtttcatt ttcacttaat ttagttgtca cgtgttcgtc aattcgtaaa
    50401 aacggtaaat atataaacac acttgcggct ataattaata tttgtaaaac agctgctcgc
    50461 cagtctccgg ctgttgctaa aaatcccgat ataactggtg gcgtagtcca aggaatttgc
    50521 actactacat gattaattaa tcccacagca gttgcaaaat atgctactgt caaagaaaaa
    50581 atcggtgcaa gcacaaaggg aataatcaga ctgatattaa atacaatagg caatccaaaa
    50641 ataataggtt cgcttatatt gaatatagcg ggagttaccg ataatttagc aaagtcctta
    50701 taatcttttc tacgactgaa gataaatatt gcaatcaata aagcaatcgt atttcccgat
    50761 ccacccatta ccgcaaaggt atctttaaag gccatattga taatatgtgg aatttgctct
    50821 ttgtttgcat aagctgccat attttcttgc atattctgaa tcaaaaacgg atctaacaat
    50881 gaacctgata ttactgcttg atggatacca aatccaaaaa atagattagc aatagatgta
    50941 attaacaaaa atcctggtaa accagttgta attttactta atggctctgt aactatcgtg
    51001 gtaattagct tatttatatc catattaaac aattgaatga ttataaatga caataaagca
    51061 aacgaggtaa tcgtaataat gattgggatt aatacattaa aagactttaa aacagcggga
    51121 ggaatatttc cggatagctg aatctgtagt ttcttattat tagaaagttt aataaataat
    51181 tctgtagccg ctaatccaac aaatatacct acaaacattc ctgaagctcc tgtataggat
    51241 ataggtatca cacccgtcac ttcaacagct ttatccgcat tttcaggagt aaacatcatc
    51301 tgcataggtg ttaatacaac aaatgtcgaa atagcaacta ataccgctgc aatatgattt
    51361 gtataatttc tgcttgcaca catatgataa gatactgctg ctacaactaa taatgtagtt
    51421 atgcttaatg taccattggc aattgaagta ccaatttgtt gtaacgtggt caatgtacta
    51481 gtatcgacaa tttcccccat gaatcctgtt ggttctaaga tcactgaatt aataagtgtc
    51541 acaaatcctg ctaacatcat aaatggcata aatgtagcaa acgcatctcg caaagttctt
    51601 aaatggatct gattccccaa cttagtcgca actataccaa atttttcaaa tacaatgtcc
    51661 ttcctgctac ttttgcccat attttttctc ctttatgctt attgtaatcg attacttttt
    51721 ccacccaaat aatacatcac ttatcaaaaa aagtaaatac ttttaattaa atatatatat
    51781 cttttttagt ataaaaaaat aagctctgaa aaaataattt tcccgagctt atttttcaac
    51841 aaaaacagct tcaaaaacaa agtcttgata taaatgtctc gataccgaat actcgaaagg
    51901 tttaccagac gatagagcaa caactttgtc tatctcaatc ataaattcac ctggatctat
    51961 ttttaatatt tttgcgtcaa attcattcgc agtatctcct ttgatacgta tgtgcgaagt
    52021 ctttatttcc agacccagtt catttgtgat ataagcatat acagatttct ttaaatgttt
    52081 tggttccaag cctggtatga ctgctaatgg catgaatgtt tgctcaatcg agtatggttg
    52141 acaatcaata aaacggcttc gtacaatatt atatacatag tcttttacac tgatacttaa
    52201 tttatcagct acttttttcg atggaagttc aattgagaaa gtatggattt ctgaatctac
    52261 gtgttcttca ccaaccacgc taaacaaacc ttcgtttgga ccaagaactc gtgagttatc
    52321 aattggtgtt ctgacgactt cagtcccata cccacttcta cttcgcaaaa ctccttcgga
    52381 aacaagtaag tctacgcctt tctttactgt taacttgctt gtatgataaa taactgccaa
    52441 ttcatctcct gtaggaagtt tctcaccaat tttatactct ccatttacaa tttttttctt
    52501 tatatctaag taaatttcca tatacttcgc aatttttttc aatggtgacc ctccaatcaa
    52561 aaacatatct tcatctatac tttaacataa ataaaccgat aaaatccaca tcaaaattat
    52621 acacacaata aaaaagatat atatatttaa acaaaactat ttactttaag ttgtaagcgg
    52681 tgttatcatt aagtggaaac aggaggtaaa aaaattatga caactatgaa aaagctaact
    52741 tttccagatg atttttggtg gggttccgct tggtcagctg agcaagcaga aggaaaagga
    52801 gaaacaggaa aagcagagac tatctgggat cgctggttta aagaacaacc ttaccggttt
    52861 tatcaacgaa tcgattcctc tattacaacc gatcacatcc atcaatataa agaagatata
    52921 gaactaatga aaaaaactgg gcataactcc tttcgcgttt ctatttcttg ggcacgaatg
    52981 tttccaaatg gcggtaccgg aaaagtaaat cccaaagcca tttctttcta tcgaaaatta
    53041 ttttccgaaa tgaataataa cggaatcaaa gtatttgcca acttatatca ttttgacatg
    53101 cctgctactc ttcaagataa aggaggatgg gaatcacgtg aagtagttga tgcttatgtt
    53161 cattacgctg aaacttgttt taaagaattt ggtgacttag tttgtcattg gtttaccttc
    53221 aatgaaccac ttgggcctat tttaggtact tatcttgaag attttcatta tccaaacctc
    53281 attgacttta aacgcggagc gcaagcagca ttcttcacta tacttgcaca cgcaaaagca
    53341 atacaagtat ttaaagaact agctcttcct agtaaaatag gcgttattct aaatttgagt
    53401 cctacttttc ctcgtagtca aaatcctgct gatgtgaaag cagcagaaat cgctgattta
    53461 ttttatacgc ggagcttttt agatcctatg gttaaaggga atttttctaa aaagcttatc
    53521 ctgttattac aagaatataa tcagcttcct gaagattata cgaaagaaga tcttgtatgt
    53581 atctacgaaa ataccgctca aattcttgga ctaaactatt acgagccacg tcgcgtcaaa
    53641 tctcgttctt ctatggtgga tacatctagt ccatttttac cagagtggtt tttcgaacca
    53701 catatcatgc ctggacgacg aatgaacgaa taccggggat gggaaattta cgaaaaagga
    53761 atttatgatt tgtgcatgga tatcaagaac cattatggaa acatcgaagc atttatctcg
    53821 gagaacggaa tgggcgttgc ggatgaagaa cgtttcatgg atgatacagg gcaaataatt
    53881 gatgattatc ggattgagta tatcaaagat catcttgctt acttatggaa ggcaataaat
    53941 gaaggatgta acattaaggg gtatcatctt tggacattta ttgattgctg gtcatggatc
    54001 aacgcctata aaaaccgata tgggctggtt tcactcgacc ttactactca aaaaagaaca
    54061 attaaaaaaa gtggcgaatt ttacaagcaa ttaagtgata caaatggatt tagatacgat
    54121 gtagacttgt tagttgaata atattctaaa attccacttg aagtgaacac attagaaaac
    54181 aagaataatg agaaatacca ataacaaata ctttttcttc atacttcatt tatacttgtc
    54241 agtataagag actgtgacaa atgtagtgat cctcagaagt taggtctgga aatctaactt
    54301 gagaagattt ttttaaacta cgtccagtct tttttatttg tttaaaattg aagttgacaa
    54361 gtcttcatta aaaatttaga atattcacaa gtttgacgac aattatgaaa atttatgtcg
    54421 tgctaaagga gttttgttat tggatgaaaa gttagatata aaaactgcga tcaaagacac
    54481 gttacctacc gtttttggtt atatcggtat tggacttgca tttggtatcg ttgggaaagc
    54541 tgccgggttt catccgctgg tcgtcacgtt gatgtccctg ctgatctatg ctggttctgc
    54601 ccaatttatc acggtcagca tgcttgctag ccatagtcca ctgctctcca tcgttctctc
    54661 gacctttcta gtcaattccc gaatgattct gatgagtatg acgattgctc cttatttcaa
    54721 gaaaaatcgg ctgcttcaaa atctgctgat tggcacattg ctgacagatg aaagtttcgc
    54781 actgggaatg aataaattga actatactgg acaaaagtta aactttcgtt ggatgaatac
    54841 agctaattgg atttcttatt tgacgtgggt gggttcttct cttgttggcg cacttttagg
    54901 gaattttatt actgatccga aaaagtttgg tttggatttc gccatcgtcg ctatgtttat
    54961 cgggttactt tatcttcaag tcatttctga tagaaacacc tctaaacgat tacagttgat
    55021 cctgattggt ctcactttga ttctggtata tgtcggactg atttttatcc caagtaactt
    55081 agtgatttta gttgtaacac tgattgggtg cggattgggg gtgtggatca aacatgcctt
    55141 cttttgagtt tacattgctg actattttag gttgtacttt agcaacttgg ctttctagga
    55201 tcctaccatt tgtcttactg aaaaaattcg atttgccgca accgttgctt gagtatttaa
    55261 gttttgtccc gatcgtcatt atgtctgctt tatggttcag cagtctattt acccaaaata
    55321 tcggacatct gccgcagatc aatctcgaaa atgctttggc ttcagttcct acacttttag
    55381 ctgctattct ttcaaaaagc ttattggtga tcgtcttggt aggaattctt tccctttcgt
    55441 tgattcgctt attataaata aaaaaacaag agcttatggc aaattactgt catgcgctct
    55501 tgtttttatc aaagtcaaat aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg
    55561 accactaaca acgacttttc ctaatatatg tcctgtttct acgtctttgg ttgcttgttt
    55621 caagttcttc gcattcatcc catctgaata gaccttgctc aatgtactgc ggataatccc
    55681 ttcatttgct aaagcagcaa tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt
    55741 atagtctgtt ttagcgaaca tatactccca gtcgaagctg atagattggt ttttccaaac
    55801 acctatatct aacggctctt tgatccctac gatcgttccc acatgtccaa atggtcggac
    55861 aaggtgtttg atttgatcaa agtagcgggt aatatcgaat aatacagcga catactctac
    55921 ttgatcgatt cctaattcac gaaaactttt acttaagtcg tgatgataat cgattggata
    55981 gtctacacct gtttttttca gccattcaaa attcttcgga cttgcagtcg cgtagacttc
    56041 taggccagcc caatgcgcta gttgattcaa aatcgaaccg acgcccccag ctccgttgat
    56101 cactaagatt tttttcccgc ggttggcatt ttcttctgga gtcagaccaa atttttcaaa
    56161 taacaattca taagcggtca atgaggtcaa cggcaaggct gcggcttctt catctgaaag
    56221 attttttggt gcaagagcga caatacgttc gtcaaccagt tggtattctt gattgcttcc
    56281 tgctcttgtc gttgtacctg cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc
    56341 atcacctaca gcaacaactt tcccgactcc atcaaaccct aagatacgta aagcatttct
    56401 tatcccgtta tcttggcgaa gtttcgtatc gacaggatta acagatactg cgttgacttt
    56461 taccagaaga tcccgtgaac tgggtacagg tatctgttct ttttcatcaa taaagctttt
    56521 tggatcatcg atcgacaacc cttcataaaa accgactacc ttcattaatt ccttttctga
    56581 catacgaatt cctccttagc ttcatcaagt tggagcatcc atacagtttt catttcaata
    56641 tatatagtat gctatttgtt ccacttttat tcatcataat tattctctta cttaaagtaa
    56701 actatttgca cttttttggt taaaaaagag gttgcgacaa gacttttgtc acaacctctt
    56761 ttaaacggtg ttcaatcaac tgaccctcgg tattcgctca aaaaacgaca aaatatgagg
    56821 agctattttt gtttggctgt tcttattact tgatgcagga cagctttttc acgacctcta
    56881 attcacggtg ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg ttctttttca
    56941 aaggtgtcta agatctctcg tacttcatca gtagatttcg tattcatcaa ctgaactcgc
    57001 aagtcatttg ctcctggaaa tcctttgata tagattttaa agaaacgatg tagtcctgtg
    57061 atggaacgcg ggatgatttc tgaataatga tcttgcagat ctagttgcag acgaagtagt
    57121 ccgatcagtt ctttttctgt atgttccctc ggttcttttt caaatgcata aggattcttg
    57181 aaaatccccc ggccaatcat gatgccatct acgccatatt tttcggccaa ttcttctcct
    57241 gtctggcgat ctgggatatc tccgttgatc gtgatcagtg tttgcggagc gatttggtca
    57301 cgtaaagcaa tgattttagg gatgacttcc caatgtgcat ctactttact catctcttta
    57361 cgcgtacgca aatggacaga aagattggct atatcttgat gaagtaggtg actgatccat
    57421 tcttccatct cagacatttc cgtatagccg atacgcgtct tgacgctgac aggcaaaccg
    57481 ccagctttag ccgcttcaat caattgtgcc gctacttctg ggcgaaggat caagccactg
    57541 ccttttccac gttctgctac attagggact gggcagccca tgttgatatc gattccttgg
    57601 aagcccattt ctgccatagc aatgctcatt tctctaaaaa attctggctt gtctccccaa
    57661 atatgggcaa ccatcggctg ttcatcttct gtaaaggtca atcttcctcg tacactgtct
    57721 tttccatctg gatgacagaa actgtctgag tttgtaaact cagtaaagaa gacatccggt
    57781 gctgctgcat gctttacgac atggcgaaat acaacatctg ttacatcttc cattggtgct
    57841 aaaacaaaaa aaggctttgg cagctctttc caaaaattag tagtcattta tttgttactc
    57901 tccattctat caagcagtga agtcgcaatc tttctcaaaa acattgtcat tatactagga
    57961 aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt ctgattgagc aaaaaaagac
    58021 tgtgacaaaa atcttatcac aattcctttt ccggaataaa tggtgttcaa aagcttgcat
    58081 ctgcggtaat aagtccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
    58141 tattacttga tgcagaacag ctttttcaca acctctccac taaacggtgt tcaatcagct
    58201 gaccctcggt attagctcaa caaacgacaa aatatgagga actattttcc cgtttgtttc
    58261 tctaatactc aggtcaaaac gctgatttca caacctcttt tctaacttct caatcctctt
    58321 cctttattga tcaaatacca agtaacggcg tacaaaacaa cgatgaatag cacaagtacg
    58381 atcatcgatg ttgctactgg cacatcgatt gttccaagga atccgtaacg gaaaccggag
    58441 atcatataaa cgatcggatt gactttcgaa acagcttgcc aaaatggcgg caacatagag
    58501 atggcataga aaacgccacc tagataagtt aacggctgca agacaaatgt aggcacgatc
    58561 gatacatcgt cataagactg agcaaaaatc ccgttgagca gtcctgctaa cgaaaacaag
    58621 attgccgtca tcaataatgt aatgatgacc atgaaccatg aatacacatg aagtggtaca
    58681 aagaacaagg aaatgatcgt tacgagagag ccgacgagaa tactgcgacc cactccgcca
    58741 atcacaaagc cccaaatgat cacatgcgta ggaacaggcg ctaccaagat ttcttcgatg
    58801 tttttctgga atttttgaga gaagaaggac gaagaaacgt tggcatacga actagtgata
    58861 gcagacatca taatcaaacc aggcacgatg aattccatat atgaaaaacc acccatgtca
    58921 ccgatccgac caccgatcat ttttccgaaa ataacaaagt acaaggatgt cgtgatcaca
    58981 ggcggaacca gtgtttggac ccagatacgc aaatagcggt tcgtttcctt gatagctaaa
    59041 ctttttaatg cagtaaaata aagattaaac atttttctcc tccgtatgat tattttcttc
    59101 tgtgattttc aaaaacagtt cttctaaacg attcgactta ttcctcatag aaagaacttt
    59161 gattccttgt cttgtcagct gatcgaagat tccgttcacc ccttgatttc gttcaacttc
    59221 gacagcgatt gtctgttcat cttcaaatgt acaagcgtat ccttcaatca caggtttggt
    59281 ctcatatggc gctaaatcaa agataaatgt ttcaaattgc agtttggata gaagctcttt
    59341 catactagta ttctcgatca gttcacctga ttggatgatc ccgatgttgc gacaaagcat
    59401 ttcagcttct tctaaataat gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc
    59461 tcggagaaac tcccacatct cgcgtctcaa ttcaatatcg actcctgctg ttggttcatc
    59521 caagatcagc aaacgtggtt catgcatcaa tgccctagca atcatcagtc ttcgcttcat
    59581 tcccccggaa agcatacgtg cacggacatt tctcttttcc caaagattgg actgcttcaa
    59641 atatttttcg ctgcgtttca tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt
    59701 cacgacgatt tgctggaccg tttcaaaggg attgaaattg aactcttgcg ggactaatcc
    59761 aatctgttgc tttgctcgga ctagatccgt atccagatca taaccgaaga cattcacttt
    59821 tccagatgtt ttattgacta aagaagtaat gattccgatc gttgtagact tcccagctcc
    59881 atttggtcct aatagcgcat agaaatctcc ttctttcacg gaaagatcga ttcctcgcaa
    59941 tgcttcgacc ccacttccat aaaccttttt caaattattt atttcaagcg catttgccat
    60001 ttatttccta tctccttata gacatgattc tttattgtat catggaatac agcagatgaa
    60061 aaaacaactt ggttccagcg aaaaatcagg ttgctgggct caggaaaatc tcctaatgaa
    60121 agacatcaat cggacgaagt cagacaacct tttcagaaaa aacaaatcga agataaagga
    60181 ttactattca tatgtgcaac aaaaggagcg tgagacaaaa gtaaagaaca cttttgtctc
    60241 acgctctaaa cacgtataaa cggcggaagc aaaagccaag atttttgatc caataaatac
    60301 tttcgatcac ttcgcctatc ccaatacatg ccactgccgc ttttcatcaa ataacgcggc
    60361 ttccgttttt gcggtttcta ctaaagtctc tgggtaatcc aaaactttca gcaaggcaat
    60421 cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga ttccttttcc
    60481 ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa tctctgtcaa
    60541 ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc accgaacgac
    60601 tgaagcggaa gaagcaatcc gttcgatcgt atttgtccct tttaaaatct catctacgaa
    60661 gcagtagcag cgttctttgg aagccacttg gtccaacaaa cgtttgatcg atttgatttc
    60721 tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg aagtcaacac
    60781 atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct gtgctagaat
    60841 acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg aacccgtgac
    60901 taatgtattc ttctgccagt tgaccggatt cactaccgct tcttttaaca atggatgata
    60961 gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga tgggcatata
    61021 agtacggtaa ttcaatattg cagcggctac ttctaattct cccagcaatt cccacagctt
    61081 gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg agataaatgg
    61141 cagcatgaac atgatgttga catattcaaa aatcaactct gcttcactcc cactcttcat
    61201 acgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct gtttcagctc
    61261 ttcttgaagt ggtgtcggca gttttgctag ctgattggcg gctgaaatcg ttgaaaccaa
    61321 ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat aatagaccgt
    61381 attgaatact aacccaccga ttgccaaata gattcctagt acttgtccta aaagaagcaa
    61441 acccaaaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc gtccaatctt
    61501 tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt ttcctaaacg
    61561 agcgaattga tattggattt tttctctcag ttgaggattt tcctgataaa aagcaatcag
    61621 tttttctaac tgctgatctt caccaaaatc aaagctgcgt aaacgttgat acaacgcttc
    61681 tgaacctaca ctagaatagg tactgttgat tccttcgaat acttcgaaca tatccaggtc
    61741 ataccaagta agatcatcga tttcgctatc ccatgaacgg aacttcttct ccgtgagcca
    61801 tgcttccttc aagctttctt ctttatcaaa tctcggttga taaggaattt tttcccattt
    61861 gctacgaact gtttccttta atttcaaacg tgaaaaaatg tctaaagcaa ctaataaaat
    61921 aacagctgca ataataatta aaacgataaa ttctctttga cccatatccc tctccgttcc
    61981 tttatttgtt agctttagta tagcactgtt gcgctatttg aaaagagggt aaaaaaacac
    62041 ctttcaaaat aaaaggtgtt tttttcttgt atattacata tttatctctc atttatcaaa
    62101 attgacaaat aatcataggg aggaattgtt gttaattctt gtttattctc cagtgcattg
    62161 acaactgtcc caagggaaag gatagcctca ggtactattt tttcttgttc tgttggatta
    62221 gctaagcgaa gaacaaatcc ttgttcatga aaataagaag gataaatcga tgatacaatt
    62281 aagtcttcag gtaattcaat caatgaaaat ttacgaggaa catcaagttc attttgtaat
    62341 ggccaaatct tgttatctaa cctgtgaata aatacattca gtttctgctt ttgataagaa
    62401 atagattgtt ctaaccgttt ttctgcctgt tttgccactt tgaactcatt aaatcttcct
    62461 tcttctacat taaatccaaa agtgacttta tactcaccta tctcttgagc tagcggtgtt
    62521 tccatcatga catgcccttc atttgtcgta tctcctgaag ctcgcccagg tcgccaagca
    62581 agattaggtt ttccaagttc ccctgttgtt gaaaataagg ttatgattaa ttgattttct
    62641 atacgttcat actcttttaa accatctgca taaaacgtaa aataatgatc attttctgcc
    62701 acagagacac tcttatcgaa tatttctaaa ttaacaggtt tctctaccat agtttctgac
    62761 caattcggag gaatgacatc aggggtattt tcaataaatc cgttttgaat ttgtgcaatt
    62821 gatttttcag atttatctaa aacattaatt gctaagcgca tacgatgcga gtaaacagta
    62881 ttatgaattt tgagtgtgcc ttcaacgatt tgactttctc ccagactgaa cgatatttca
    62941 taagatattt cttccatttc gggtttttca gataaacgat cttttaaatc tttaggaagt
    63001 tgagcttttc cgtaaacaac taatgtttct tgtaaagaat ctttatatac tttagctgtt
    63061 tctaatttta gagatagttc tgtatctcct tctaatggtg aataatcata cgtatcgcca
    63121 tcatttgctg aatcgatcaa atgaacgaaa tcaagatatt gtctatctcc aactatcaaa
    63181 ttcaattgcc catctttaaa agaaagttta tatttattgt tacttatctc agttttgtct
    63241 atctcgattg ctgtttctaa tttttcatta tttttctcaa attcaacaac agtgtatcct
    63301 agtgctggca catttacatt tatttttatg tcaagttcat aataagctgg ttcatcaaaa
    63361 tattcgaaac ctgtagcaat catccgataa gcatttttcc gttctggaaa ataaacttct
    63421 ttttcaatga tattataggg actacctttg aagtgtattt tctttgaatc agctactaca
    63481 tgcacgattt tcgatccact ataattctca gtcgttgtgt tgaaaacaag gacttcattc
    63541 gattttagct ttaatgcatc agccatccgt tttacaataa ggttttcgat tccgtcagct
    63601 atctcattag cttctttgat tcgatggaaa atatcttccg caacattatc tgaaacacag
    63661 ccaccaatac tatcatgagc ctggttttgc aaaatctttt tccataaccg acgaagtagg
    63721 cctttactga tatggatacc atttctttct gcaatcagca tgagcggctc aactcgtttt
    63781 agaatttttt gttccaattt aaaattttct attttcattt ttgttcttac tgagccaata
    63841 gaacgatgaa cacgagagta aaccggctga cgaagttcac ctttatattc aggtaattct
    63901 tttttgtttc tgataacttc tacataagaa ggataatctg aaataatatt gtggaattta
    63961 ctgtatttat tcaacttctc tacgacttga tcaaaattta aaatcattga cttttgatct
    64021 acacccgaag gtactaaaat atctttgttt tttccttgca tatgaagcat ttcaactgct
    64081 tcatccaact tttcttttgc aaattcagga atatctttaa ttgcgtctaa ggtcatcatt
    64141 ccagtcatat aaccaaatgg aaaattcatg gcataaacaa atttatctcc caatcctttc
    64201 cagataaaat aaggagatgg aaccatcgtt tcaaagttta atcctcgcca acacatgaaa
    64261 ttatcgattt ctacttgatg caaaagtgta ggcaaattcg cattgaaccc aaacgtgtca
    64321 ggcaaatacc ctaccatcat tggatctcca tatttaagga tcgtatcact gattccaatc
    64381 attaagtttc ttaaaattga ttcagcatcc actaataagg catctgtttg tgcataccac
    64441 ggtcccacga atagttgtcc tgttttcatc agtcgttgga taaccttaag cttttgcgga
    64501 ttgatctcca aatactcatc gacgattgaa gtttgtccat ctaaacaaaa gtttacttta
    64561 gggttccttt ccaattcatc tagaacttca gtaaagacct gatcagacaa tacgatggca
    64621 tcttcatttg taaaatacca ttctctgtcc cagtgcgtgt gttgaattat atgtgcattt
    64681 accatatcag attctcctac tttccttctt ctactgaatt tacttgttgt tcttttttat
    64741 acttgatgga ttttagtgca ttgaccatta acgctgtaac cactccccca ataacaattg
    64801 atactaaata taaaggtata ttcgtaacaa aaggaattgc cataaaggtt gaaagcattg
    64861 tttcttgagt aacaccaaaa aatccattta gaccacatgc tactgcagtg cccaccacga
    64921 tgcttggtat tactcgtaaa ggatctttca cggcaaacgg gatcgtccct tcagtaatcc
    64981 ccacaaggcc cataatcata gctgtctttg cattatctcg ttccgattca tcataaagtt
    65041 ttttgtttaa aattgttgct aaaaacattc caaatggcgg aatagcaatt cctaataaca
    65101 tggctgtact aggtccaaag cttccgcttg aaaaagaagc aaccccaaaa gcatatgcga
    65161 ttttattgat aggtcctccc atatcaaacg ccatcattcc tcctaaaata gccgacatca
    65221 aagcagcatt tgctcctgac atgccttcta gccaattagt cagtcctgtt gtcattgctt
    65281 gaataggcgt agctagtaca taccataata tgattccaac aactaatgta gtaacaaaag
    65341 gaatcagcat aattgatttt agactaataa cttgcatcgg tacttttact tttttcaatg
    65401 cattagctaa atagccacaa gctaatccag caaccattcc tccaataaat cccataccaa
    65461 gattttgaca catcatccca gtgataaaag ctggtgctag accgggacgg tcagcaattg
    65521 aatatgcgat ataggctgaa atcatcggta cgataaactg taatcccgtc tgaccaatca
    65581 ttcgcaggac tccccagaat gtatccgtct ggtcccaaac acctgtgcca ccaaaaatga
    65641 ctgcaataga agttagtaac cctgctgcta cgacaagcgg gatcatatag gaaaccccac
    65701 tttggaaatg atttagaatt tctcttcctt gtttcttgaa ttccattagt aactccccca
    65761 cgatatattt tttatttgtt aattaatgca attgcttcat caatcgtctc ttcaccatct
    65821 tttactacct ctgaggtccc tactgttaaa atcggtttac cgtcaaagcg acctcgttct
    65881 gaaacatttg tatctactgc aaatatgaca acatctgcct cggaaacatc cgaaattgaa
    65941 agtttgtttt ctatccccat agccccctgt gtttctactt taatatctaa ccctttcttc
    66001 ttcgcaaact tttctaaatt agcttttgcc atataagtat gggcaatccc tgcgatacat
    66061 gctgttactg ctactatttt tgtcatatga aattcctcct tttaaaatga tagagctttt
    66121 accaattctt ttttatttct cgaatctttt attcttttaa caaactcgtc atccatcaat
    66181 cttccagata attctgctaa aatacgtaaa tgattagtac ctttatcagt gtcttttatt
    66241 gctaacaaaa agacgagatc aactggttga tcatctaatg cgccccattc aacagaccgc
    66301 tttaacttgg ctacagccac tgttgattca attactgcta ggcttttgcc atgaggaatg
    66361 gcgatatttt tcccaattcc tgtggtcata tggttttctc tgtcaaatac gtctttttga
    66421 aattgtttgt ggtcagacac aaacccatta gtctctaaca atttcgataa tttttcgact
    66481 acactttttt tatcagatgc atttaaatct atcactatat tatttatatt gattttttca
    66541 ctgatcattt ataagccttc tttcaata
//
LOCUS       DAZKOM010000006        62506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000006 DAZKOM010000000
VERSION     DAZKOM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.006"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..529
                     /locus_tag="ACG8NF_000375"
     CDS             11..529
                     /locus_tag="ACG8NF_000375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0470890.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            657..1340
                     /locus_tag="ACG8NF_000376"
     CDS             657..1340
                     /locus_tag="ACG8NF_000376"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0470891.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1649..1722
                     /locus_tag="ACG8NF_000377"
     tRNA            1649..1722
                     /locus_tag="ACG8NF_000377"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Arg,seq:tct)
     gene            1881..2240
                     /locus_tag="ACG8NF_000378"
     CDS             1881..2240
                     /locus_tag="ACG8NF_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0470892.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2363..3994
                     /locus_tag="ACG8NF_000379"
     CDS             2363..3994
                     /locus_tag="ACG8NF_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0470893.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4056..4697)
                     /locus_tag="ACG8NF_000380"
     CDS             complement(4056..4697)
                     /locus_tag="ACG8NF_000380"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0470894.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4883..4959
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4979..5287
                     /gene="rplU"
                     /locus_tag="ACG8NF_000381"
     CDS             4979..5287
                     /gene="rplU"
                     /locus_tag="ACG8NF_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0470895.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5314..5658
                     /locus_tag="ACG8NF_000382"
     CDS             5314..5658
                     /locus_tag="ACG8NF_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0470896.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5671..5964
                     /gene="rpmA"
                     /locus_tag="ACG8NF_000383"
     CDS             5671..5964
                     /gene="rpmA"
                     /locus_tag="ACG8NF_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0470897.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6398..9205
                     /locus_tag="ACG8NF_000384"
     CDS             6398..9205
                     /locus_tag="ACG8NF_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0470898.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9396..9887
                     /locus_tag="ACG8NF_000385"
     CDS             9396..9887
                     /locus_tag="ACG8NF_000385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0470899.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9990..10979
                     /locus_tag="ACG8NF_000386"
     CDS             9990..10979
                     /locus_tag="ACG8NF_000386"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0470900.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11012..11815
                     /locus_tag="ACG8NF_000387"
     CDS             11012..11815
                     /locus_tag="ACG8NF_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0470901.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11836..12750
                     /locus_tag="ACG8NF_000388"
     CDS             11836..12750
                     /locus_tag="ACG8NF_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0470902.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12833..13207
                     /locus_tag="ACG8NF_000389"
     CDS             12833..13207
                     /locus_tag="ACG8NF_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0470903.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13346..13591
                     /locus_tag="ACG8NF_000390"
     CDS             13346..13591
                     /locus_tag="ACG8NF_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0470904.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13763..14827
                     /locus_tag="ACG8NF_000391"
     CDS             13763..14827
                     /locus_tag="ACG8NF_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0470905.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15073..15498
                     /locus_tag="ACG8NF_000392"
     CDS             15073..15498
                     /locus_tag="ACG8NF_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0470906.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15491..15940
                     /gene="nusB"
                     /locus_tag="ACG8NF_000393"
     CDS             15491..15940
                     /gene="nusB"
                     /locus_tag="ACG8NF_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0470907.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16037..16891
                     /locus_tag="ACG8NF_000394"
     CDS             16037..16891
                     /locus_tag="ACG8NF_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0470908.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16892..18229
                     /gene="xseA"
                     /locus_tag="ACG8NF_000395"
     CDS             16892..18229
                     /gene="xseA"
                     /locus_tag="ACG8NF_000395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0470909.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18234..18458
                     /locus_tag="ACG8NF_000396"
     CDS             18234..18458
                     /locus_tag="ACG8NF_000396"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0470910.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18460..19350
                     /locus_tag="ACG8NF_000397"
     CDS             18460..19350
                     /locus_tag="ACG8NF_000397"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0470911.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19359..20177
                     /locus_tag="ACG8NF_000398"
     CDS             19359..20177
                     /locus_tag="ACG8NF_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0470912.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20284..20733
                     /locus_tag="ACG8NF_000399"
     CDS             20284..20733
                     /locus_tag="ACG8NF_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0470913.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20751..22433
                     /gene="recN"
                     /locus_tag="ACG8NF_000400"
     CDS             20751..22433
                     /gene="recN"
                     /locus_tag="ACG8NF_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0470914.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22488..22604)
                     /locus_tag="ACG8NF_000401"
     CDS             complement(22488..22604)
                     /locus_tag="ACG8NF_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0470915.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22677..23633)
                     /locus_tag="ACG8NF_000402"
     CDS             complement(22677..23633)
                     /locus_tag="ACG8NF_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0470916.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23804..24172
                     /locus_tag="ACG8NF_000403"
     CDS             23804..24172
                     /locus_tag="ACG8NF_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0470917.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24342..24773
                     /gene="mraZ"
                     /locus_tag="ACG8NF_000404"
     CDS             24342..24773
                     /gene="mraZ"
                     /locus_tag="ACG8NF_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0470918.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24794..25753
                     /gene="rsmH"
                     /locus_tag="ACG8NF_000405"
     CDS             24794..25753
                     /gene="rsmH"
                     /locus_tag="ACG8NF_000405"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0470919.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25758..26159
                     /gene="ftsL"
                     /locus_tag="ACG8NF_000406"
     CDS             25758..26159
                     /gene="ftsL"
                     /locus_tag="ACG8NF_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0470920.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26160..28352
                     /locus_tag="ACG8NF_000407"
     CDS             26160..28352
                     /locus_tag="ACG8NF_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0470921.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28378..29340
                     /gene="mraY"
                     /locus_tag="ACG8NF_000408"
     CDS             28378..29340
                     /gene="mraY"
                     /locus_tag="ACG8NF_000408"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0470922.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29354..30715
                     /gene="murD"
                     /locus_tag="ACG8NF_000409"
     CDS             29354..30715
                     /gene="murD"
                     /locus_tag="ACG8NF_000409"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0470923.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30741..31829
                     /gene="murG"
                     /locus_tag="ACG8NF_000410"
     CDS             30741..31829
                     /gene="murG"
                     /locus_tag="ACG8NF_000410"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0470924.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31844..33064
                     /locus_tag="ACG8NF_000411"
     CDS             31844..33064
                     /locus_tag="ACG8NF_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0470925.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33220..34545
                     /gene="ftsA"
                     /locus_tag="ACG8NF_000412"
     CDS             33220..34545
                     /gene="ftsA"
                     /locus_tag="ACG8NF_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0470926.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34568..35809
                     /gene="ftsZ"
                     /locus_tag="ACG8NF_000413"
     CDS             34568..35809
                     /gene="ftsZ"
                     /locus_tag="ACG8NF_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0470927.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35826..36503
                     /locus_tag="ACG8NF_000414"
     CDS             35826..36503
                     /locus_tag="ACG8NF_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0470928.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36517..37116
                     /locus_tag="ACG8NF_000415"
     CDS             36517..37116
                     /locus_tag="ACG8NF_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0470929.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37197..37418
                     /locus_tag="ACG8NF_000416"
     CDS             37197..37418
                     /locus_tag="ACG8NF_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0470930.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37467..38249
                     /locus_tag="ACG8NF_000417"
     CDS             37467..38249
                     /locus_tag="ACG8NF_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0470931.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38270..38977
                     /locus_tag="ACG8NF_000418"
     CDS             38270..38977
                     /locus_tag="ACG8NF_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0470932.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39239..42037
                     /gene="ileS"
                     /locus_tag="ACG8NF_000419"
     CDS             39239..42037
                     /gene="ileS"
                     /locus_tag="ACG8NF_000419"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0470933.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42086..43612)
                     /gene="zwf"
                     /locus_tag="ACG8NF_000420"
     CDS             complement(42086..43612)
                     /gene="zwf"
                     /locus_tag="ACG8NF_000420"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0470934.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43627..44274)
                     /locus_tag="ACG8NF_000421"
     CDS             complement(43627..44274)
                     /locus_tag="ACG8NF_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0470935.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44458..44787)
                     /locus_tag="ACG8NF_000422"
     CDS             complement(44458..44787)
                     /locus_tag="ACG8NF_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0470936.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44964..45692
                     /gene="sfsA"
                     /locus_tag="ACG8NF_000423"
     CDS             44964..45692
                     /gene="sfsA"
                     /locus_tag="ACG8NF_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0470937.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45708..46721
                     /locus_tag="ACG8NF_000424"
     CDS             45708..46721
                     /locus_tag="ACG8NF_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0470938.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46721..47998
                     /locus_tag="ACG8NF_000425"
     CDS             46721..47998
                     /locus_tag="ACG8NF_000425"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0470939.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48061..50763
                     /locus_tag="ACG8NF_000426"
     CDS             48061..50763
                     /locus_tag="ACG8NF_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0470940.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50915..51232
                     /locus_tag="ACG8NF_000427"
     CDS             50915..51232
                     /locus_tag="ACG8NF_000427"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0470941.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51262..51582
                     /locus_tag="ACG8NF_000428"
     CDS             51262..51582
                     /locus_tag="ACG8NF_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0470942.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51690..53150
                     /locus_tag="ACG8NF_000429"
     CDS             51690..53150
                     /locus_tag="ACG8NF_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0470943.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53218..53439
                     /locus_tag="ACG8NF_000430"
     CDS             53218..53439
                     /locus_tag="ACG8NF_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470944.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53470..53652
                     /locus_tag="ACG8NF_000431"
     CDS             53470..53652
                     /locus_tag="ACG8NF_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0470945.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53652..54065
                     /locus_tag="ACG8NF_000432"
     CDS             53652..54065
                     /locus_tag="ACG8NF_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0470946.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54188..55369
                     /locus_tag="ACG8NF_000433"
     CDS             54188..55369
                     /locus_tag="ACG8NF_000433"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0470947.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55440..55604)
                     /locus_tag="ACG8NF_000434"
     CDS             complement(55440..55604)
                     /locus_tag="ACG8NF_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470948.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55758..55831
                     /locus_tag="ACG8NF_000435"
     tRNA            55758..55831
                     /locus_tag="ACG8NF_000435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55792..55794,aa:Arg,seq:cct)
     gene            complement(55900..57039)
                     /locus_tag="ACG8NF_000436"
     CDS             complement(55900..57039)
                     /locus_tag="ACG8NF_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0470949.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57211..57345
                     /locus_tag="ACG8NF_000437"
     CDS             57211..57345
                     /locus_tag="ACG8NF_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470950.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57338..57973)
                     /locus_tag="ACG8NF_000438"
     CDS             complement(57338..57973)
                     /locus_tag="ACG8NF_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470951.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58086..58721)
                     /locus_tag="ACG8NF_000439"
     CDS             complement(58086..58721)
                     /locus_tag="ACG8NF_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0470952.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58755..59216)
                     /locus_tag="ACG8NF_000440"
     CDS             complement(58755..59216)
                     /locus_tag="ACG8NF_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0470953.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59346..59777)
                     /locus_tag="ACG8NF_000441"
     CDS             complement(59346..59777)
                     /locus_tag="ACG8NF_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0470954.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59795..60115)
                     /locus_tag="ACG8NF_000442"
     CDS             complement(59795..60115)
                     /locus_tag="ACG8NF_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0470955.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60414..61190
                     /locus_tag="ACG8NF_000443"
     CDS             60414..61190
                     /locus_tag="ACG8NF_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0470956.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61205..61408
                     /locus_tag="ACG8NF_000444"
     CDS             61205..61408
                     /locus_tag="ACG8NF_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0470957.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61424..61762
                     /locus_tag="ACG8NF_000445"
     CDS             61424..61762
                     /locus_tag="ACG8NF_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0470958.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61749..61928
                     /locus_tag="ACG8NF_000446"
     CDS             61749..61928
                     /locus_tag="ACG8NF_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470959.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(61971..62441)
                     /locus_tag="ACG8NF_000447"
     CDS             complement(61971..62441)
                     /locus_tag="ACG8NF_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470960.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT        20600 a        10505 c        13105 g        18296 t
ORIGIN      
        1 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
       61 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      121 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      181 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      241 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      301 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      361 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      421 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      481 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      541 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      601 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      661 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      721 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      781 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
      841 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
      901 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
      961 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1021 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1081 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1141 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1201 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1261 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1321 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1381 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1441 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1501 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1561 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1621 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1681 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1741 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1801 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     1861 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     1921 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     1981 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2041 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2101 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2161 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2221 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2281 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2341 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2401 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2461 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2521 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2581 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2641 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2701 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2761 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     2821 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     2881 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     2941 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3001 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3061 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3121 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3181 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3241 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3301 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3361 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3421 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3481 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3541 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3601 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3661 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3721 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3781 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     3841 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     3901 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     3961 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4021 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4081 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4141 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4201 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4261 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg
     4321 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4381 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4441 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4501 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4561 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4621 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4681 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4741 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4801 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     4861 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     4921 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     4981 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5041 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5101 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5161 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5221 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5281 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5341 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5401 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
     5461 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5521 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5581 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5641 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5701 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5761 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     5821 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     5881 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     5941 tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt
     6001 ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac gaagtttact
     6061 ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt tgataactat
     6121 cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat atggtaaaca
     6181 gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt cttttaaatt
     6241 gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat agttgacgaa
     6301 acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct atagaaaagg
     6361 agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag aaaaaaccgc
     6421 tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg ctgaagtgtt
     6481 agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg aaggaaaact
     6541 gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta cgcacgtaaa
     6601 gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag aaaaaaatac
     6661 actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg gcagcatgaa
     6721 aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat taaattgttt
     6781 gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt atcattttgc
     6841 aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt gtgctgatta
     6901 tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag gtgcttttac
     6961 aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta tgctgttttt
     7021 ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact ttatggatca
     7081 tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg tacggatcgt
     7141 aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc ggcgtatccc
     7201 aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag tcgatttagt
     7261 aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt tgacagaaga
     7321 tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat tgaagtcaaa
     7381 tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg aaatctcgat
     7441 cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag caagcaaccg
     7501 cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag caccaaatga
     7561 tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat atgatatcat
     7621 cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa tcaatcattt
     7681 catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg gtttttcttt
     7741 taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag tgaccaatca
     7801 gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact ttatttatgc
     7861 tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag aacggcatac
     7921 gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg ttgcgaaaga
     7981 agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg aagactatta
     8041 cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa tcggtgtagt
     8101 cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc aacagttgtt
     8161 agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga aaacagcatt
     8221 ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca tactcttagt
     8281 agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg gaattccagt
     8341 acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa aggtctcagt
     8401 attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag gttacgcaga
     8461 aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa aagctgtttt
     8521 ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga tcgaacgagc
     8581 ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg tggaagtgaa
     8641 agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg ggattactga
     8701 tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa atatcgaagt
     8761 cattttggat cagttgctga tggaatcaga attggaggaa actgaattca tctccctaga
     8821 tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca tctttattaa
     8881 tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc agacgacggg
     8941 aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag caggaatgat
     9001 cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga caaatacaac
     9061 gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag atcagataaa
     9121 agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata atcagttaga
     9181 taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat agtgtatcgt
     9241 ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa aagttggcac
     9301 gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa tgataggggt
     9361 ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg gcacgtatag
     9421 atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac
     9481 gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt ctactaaaag
     9541 aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg attcaaatct
     9601 atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat ccccaagatg
     9661 ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt
     9721 ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa gatattgatt
     9781 cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta cctgctgaca
     9841 ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt
     9901 tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt ttgaatttgt
     9961 tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca agtcatggac
    10021 aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa gaaaacgtca
    10081 aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa ttggaagaag
    10141 cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta tggggcggaa
    10201 caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg gcaatcgtca
    10261 gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca atggaatctg
    10321 ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg
    10381 aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa cctaaaggag
    10441 cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg
    10501 actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg caaccaaacc
    10561 gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac
    10621 aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg atcgaagtag
    10681 caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat cctgaagacg
    10741 tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc
    10801 attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa gacgttgaag
    10861 cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta cctaatgatt
    10921 cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca aaagcgtaat
    10981 aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa ttttagtcgt
    11041 attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat ttcatcaacc
    11101 actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg gtatcgtact
    11161 tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc
    11221 agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg gacaaggtgt
    11281 taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag ggttgttctt
    11341 aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg cggctgctga
    11401 agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt
    11461 tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg tgcaatcatt
    11521 ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg gaatggtcgt
    11581 agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat ggccattctt
    11641 cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc taggtgcttt
    11701 aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt catcaaataa
    11761 tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact attaatcttg
    11821 aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga tcgtttagct
    11881 gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat gcaaaacggt
    11941 ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa agaagaacgt
    12001 tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat tgcttctcca
    12061 attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat
    12121 gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt tggtgaccca
    12181 gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt agctatggga
    12241 ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg ttgggcattc
    12301 atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga tgacctttct
    12361 ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt cgtattagct
    12421 gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta
    12481 gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg aatcaagagt
    12541 gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac aacattacaa
    12601 gacaacttag accaattgat tcctggttta gctgcattag gattaacatt gttatgtatg
    12661 tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt cgtagtcggt
    12721 gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg gcgttgtccc
    12781 gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga aaatggttca
    12841 atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg gtctgacaga
    12901 ttacgggcaa atcatgatag gtaataatgg ttttgagttt taccatgcgc gagatagacg
    12961 caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag tcatgctaaa
    13021 gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat acacattttc
    13081 ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg aagatagaat
    13141 ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg
    13201 caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt actttgcaga
    13261 agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata tgctaaaatt
    13321 gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg ttggagccgt
    13381 gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa
    13441 tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg aatggggacc
    13501 aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt tggtagcagt
    13561 tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa ctaaaaggag
    13621 ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa gaagttttcc
    13681 ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga aaaaagcgtg
    13741 caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca aaaaaatgca
    13801 agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt acttaacgaa
    13861 ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt ttattactga
    13921 ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta ttaaaaatgt
    13981 cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc actcattagc
    14041 ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa tcatcgatgc
    14101 ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag atgaagaaga
    14161 aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc acatcctgaa
    14221 aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact tttatatgcg
    14281 ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat tgcgttcggc
    14341 tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga tcacattgga
    14401 ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg ctattggtga
    14461 tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat tagcagttat
    14521 tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta gagattatat
    14581 ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga tcggtttaga
    14641 gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac caggaaatgt
    14701 gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta ttgaggatga
    14761 cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag aattgatcat
    14821 cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag actttgttat
    14881 agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta agagataata
    14941 aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca gttttcttac
    15001 cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt aggcaaaggg
    15061 aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca agatttagga
    15121 gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc ttctaaagta
    15181 gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt attaggacgt
    15241 gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa agtagatctt
    15301 tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat gcaggaacgg
    15361 gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa tatccatgta
    15421 gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt tgaagacgaa
    15481 gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg cattgcaggc
    15541 tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt tcaacgcaat
    15601 cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc ctgtttatct
    15661 tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag tgattgaaaa
    15721 acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg tgatcttgcg
    15781 tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg ctttaaatga
    15841 agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg tcaacggtgt
    15901 tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt ttttctatga
    15961 atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa cacaaaaaat
    16021 atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg aaaaaatgca
    16081 ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc ccggattagt
    16141 cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata aagaacgtgt
    16201 agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga tttctgaaga
    16261 cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg gtatattggt
    16321 ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta tccgccctga
    16381 aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga
    16441 caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt ctatcgatcc
    16501 ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac cgatggctca
    16561 gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg aaaatctggc
    16621 tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac attttgtcac
    16681 aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc gtgatgctaa
    16741 cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag gctggatcac
    16801 tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa
    16861 atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa ctgtcactac
    16921 attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac gcgtatattt
    16981 aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt tcagcttaaa
    17041 agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa aattacgatt
    17101 ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat atgaagcggg
    17161 aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg ctttatatca
    17221 agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa
    17281 aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg gagcggttat
    17341 ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg ttttgttccc
    17401 aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc agagagtaga
    17461 agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga ttgaggattt
    17521 atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata cgccggtgat
    17581 ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag atgttcgggc
    17641 tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag agttgatgcg
    17701 cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc agcgcaaaca
    17761 agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa gattgtatga
    17821 aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta cacagcaaat
    17881 ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac aagtggctcc
    17941 gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac gcttagaaga
    18001 gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg ttcaatctct
    18061 tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga cgttaaatga
    18121 tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga tccattatca
    18181 agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa aatatggaaa
    18241 atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag
    18301 gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa ttaagcaaac
    18361 attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg acagaatcaa
    18421 atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac tgacttttca
    18481 gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga gcatacaacg
    18541 gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa aagaatacgt
    18601 cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt tcccgtctat
    18661 caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga tgatttgcct
    18721 gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt attcggcgaa
    18781 gcattagcta tattggccgg agatggtttg cttacaggag cattccagct aatcagtatg
    18841 gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt ttgtgcggga
    18901 tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa aaagctttct
    18961 ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg ttatgccttg
    19021 ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact tcaacgatta
    19081 gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt gattggtaca
    19141 acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa tacttacccg
    19201 cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt atccgccaac
    19261 aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct gatgcgccag
    19321 atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg
    19381 tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt tcagtgatgg
    19441 ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa aaaattccag
    19501 tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga gggggattaa
    19561 aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca ttgttagata
    19621 ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca aaaatgagtt
    19681 atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat cctagagtcg
    19741 tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc ttaggaattc
    19801 cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg cctccattac
    19861 atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa ttcgaagcag
    19921 gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat caaaaagttt
    19981 tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa ttaagttatt
    20041 ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg aaagtacccg
    20101 aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat gcacacgaac
    20161 agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc ggattcttct
    20221 ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag gagcgaagac
    20281 gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga gcaagatatc
    20341 cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt tactcaagct
    20401 acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc agatggcggc
    20461 taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta
    20521 ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt gaaaacattg
    20581 ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa agaattattt
    20641 tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga ccaggcagtt
    20701 ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg atgctacaag
    20761 aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt cacgaaggaa
    20821 tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat gctttaggtc
    20881 tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc
    20941 ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg gttgaattag
    21001 ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta actggaaaaa
    21061 atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga atcggcagtt
    21121 atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct gaatcacatc
    21181 ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag agttatcagc
    21241 aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa
    21301 aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata gctgctgctg
    21361 atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt agcaattatc
    21421 aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat gaacaaaaca
    21481 gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat ctagatgtgg
    21541 agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg
    21601 gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta gaagaagtta
    21661 cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc attgagtcaa
    21721 tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt tcagaaggtc
    21781 aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag caacaagccg
    21841 cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg
    21901 aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta aaagaagaaa
    21961 atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg accaatccag
    22021 gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc
    22081 ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt tttgatgaag
    22141 tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt tctcaaatat
    22201 cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca gtcgccgact
    22261 atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct gtttcagagt
    22321 tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt gagattacaa
    22381 aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc tgagccacct
    22441 cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta catgacactc
    22501 attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca aatgacaaat
    22561 tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt caacctcgtc
    22621 attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg gcttttttat
    22681 aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa tgttaataga
    22741 actagccata taaaggcatc atccctttca gcaataggta acttaatgtt cattccatag
    22801 atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa aattttcata
    22861 atattattca gattattcga tacgattgct gaaaaagtct gattcatttg ttctactagt
    22921 tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac atcacgtaaa
    22981 tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc tttcaatgca
    23041 tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat atccatgatt
    23101 tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt atcgcattga
    23161 gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat gaattgaaag
    23221 aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa gactttcttt
    23281 aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt caaaataata
    23341 gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc aaaaggatat
    23401 tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc tgtacgggaa
    23461 ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc
    23521 agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt ttggtcagtt
    23581 tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat catgattttc
    23641 ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat ggaatgtgtt
    23701 tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg tgtaaagtat
    23761 atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca cattcacacc
    23821 acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc aatttcttgt
    23881 aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg cgattccttt
    23941 tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta tcttaccgtt
    24001 tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag catattatta
    24061 tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt ttctgttgac
    24121 aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat gaaaaaaata
    24181 gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt
    24241 atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt gggggattgt
    24301 ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg ggtgaattcc
    24361 aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg
    24421 gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat ccgatgagcg
    24481 aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta
    24541 cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa caaggacgaa
    24601 tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc gtagtcatcg
    24661 gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt tctgcagcag
    24721 cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta taagaatagg
    24781 aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga aagaaacggt
    24841 agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg gcggtgccgg
    24901 acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg cgtttgacca
    24961 agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg aacaaggtat
    25021 ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg agcatgtaga
    25081 taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag atgaagcaga
    25141 acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc agtcagctcc
    25201 tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa agattttctt
    25261 ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa
    25321 aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag tcatcccagc
    25381 tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc
    25441 agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc ggttattgaa
    25501 agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa ttgtcaaaag
    25561 tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg tcatacctga
    25621 cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt cagaggaaga
    25681 gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa aaataagaat
    25741 gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa tatgatttac
    25801 ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat caagaaatca
    25861 ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa aaaattgctg
    25921 tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg ctgcgcacaa
    25981 atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat gataaaaagc
    26041 aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa cggattaaac
    26101 aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa
    26161 tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat ccgatgaaca
    26221 atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc tttttatttg
    26281 ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca ttagctgaaa
    26341 aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga acgatttatg
    26401 atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa gcgatactct
    26461 caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat tttgataaaa
    26521 tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg aatattttag
    26581 aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg aaaaatatct
    26641 cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt
    26701 attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct cattttattg
    26761 gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt ttggagtctg
    26821 cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa gataattttc
    26881 aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga caagatatct
    26941 acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat caagtaaatg
    27001 aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct
    27061 tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg actggaaaag
    27121 atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca accatgaaag
    27181 ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa accttccaat
    27241 ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt gaaaaaggtg
    27301 tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc atactggaac
    27361 agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga caaagtactc
    27421 attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt gtcgaccgag
    27481 caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg aaagctttca
    27541 cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa gtggtggatc
    27601 cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct ttttcaaaag
    27661 aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa aactacggta
    27721 gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac ggaacagccc
    27781 aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg
    27841 tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta
    27901 agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg
    27961 attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag
    28021 ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc
    28081 aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc
    28141 agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg
    28201 taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta
    28261 gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg
    28321 aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg
    28381 gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg
    28441 ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga
    28501 ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc
    28561 ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt
    28621 ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata
    28681 aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt
    28741 ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat
    28801 ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt
    28861 gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta
    28921 ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta
    28981 ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa
    29041 cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca
    29101 atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg
    29161 gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt
    29221 aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat
    29281 acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag
    29341 gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg
    29401 actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac
    29461 cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg
    29521 gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat
    29581 ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg
    29641 tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc caattattgg
    29701 gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc
    29761 aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt
    29821 agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat
    29881 gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat
    29941 tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat
    30001 gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca
    30061 aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta
    30121 tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg
    30181 cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta
    30241 tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg
    30301 tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa
    30361 tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc
    30421 tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa
    30481 agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg
    30541 catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa
    30601 cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc
    30661 aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa
    30721 gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata
    30781 tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca
    30841 tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt
    30901 tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg
    30961 tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag
    31021 atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa
    31081 tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt
    31141 taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg
    31201 aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat
    31261 cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg
    31321 gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc
    31381 cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata
    31441 aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg
    31501 tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca
    31561 cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga
    31621 ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac
    31681 taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa
    31741 agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg
    31801 acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga
    31861 accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc
    31921 taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat
    31981 aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca
    32041 agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa
    32101 cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca
    32161 caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt
    32221 tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa
    32281 gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg
    32341 gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat
    32401 cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata
    32461 taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac
    32521 agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa
    32581 agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca
    32641 aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt
    32701 gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa
    32761 atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg
    32821 tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga
    32881 aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac
    32941 gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc
    33001 aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa
    33061 ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag
    33121 aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc
    33181 gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat
    33241 gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc
    33301 caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc
    33361 gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa
    33421 gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa
    33481 aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc
    33541 agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca
    33601 atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg
    33661 atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat
    33721 aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct
    33781 ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt
    33841 gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc
    33901 aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg
    33961 tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca
    34021 tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat
    34081 gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa
    34141 gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga
    34201 gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa
    34261 ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc
    34321 gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt
    34381 gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc
    34441 tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat
    34501 gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga
    34561 ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat
    34621 cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg
    34681 tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac
    34741 agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt
    34801 tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga
    34861 tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt
    34921 tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac
    34981 ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa
    35041 tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac
    35101 accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat
    35161 ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt
    35221 gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt
    35281 agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc
    35341 tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga
    35401 tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc
    35461 tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga
    35521 atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc
    35581 tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag
    35641 ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa
    35701 ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca
    35761 tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag
    35821 agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc
    35881 ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg
    35941 ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata
    36001 aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg
    36061 gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt
    36121 tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt
    36181 ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt
    36241 taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta
    36301 tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat
    36361 tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg
    36421 ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa
    36481 cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca
    36541 attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa
    36601 acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat
    36661 tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg
    36721 catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca
    36781 aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt
    36841 gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa
    36901 cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg
    36961 agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt
    37021 gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct
    37081 caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta
    37141 ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg
    37201 tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag
    37261 ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct
    37321 tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac
    37381 agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt
    37441 ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag
    37501 atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg
    37561 caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc
    37621 gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa
    37681 gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt
    37741 atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt
    37801 tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct
    37861 ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg
    37921 gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata
    37981 gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga
    38041 tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga
    38101 actggacaga aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca
    38161 gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca
    38221 gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac
    38281 tccattagat atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga
    38341 agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg
    38401 agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa
    38461 agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag
    38521 tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt
    38581 tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa
    38641 aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa
    38701 gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca
    38761 agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag
    38821 ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt
    38881 aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga
    38941 tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc
    39001 ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta
    39061 cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca
    39121 cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc
    39181 cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat
    39241 gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc
    39301 aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca
    39361 agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg
    39421 taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa
    39481 atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat
    39541 tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt
    39601 ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag atttcaaacg
    39661 cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc
    39721 tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa
    39781 accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa
    39841 agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct
    39901 tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca
    39961 aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt
    40021 tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac
    40081 agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta
    40141 tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt
    40201 agtccatact gctccaggac atggggaaga tgactatatc gctggtagca aatataaact
    40261 atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga
    40321 aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc
    40381 tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc
    40441 tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat
    40501 tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa
    40561 tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact
    40621 tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt
    40681 ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact
    40741 tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga
    40801 tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc
    40861 agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt
    40921 caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat
    40981 ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat
    41041 cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc
    41101 tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga
    41161 agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga
    41221 tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt
    41281 tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat
    41341 gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga
    41401 ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca
    41461 aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca
    41521 tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga
    41581 gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt
    41641 catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca gcaaattgat
    41701 tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt
    41761 aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt
    41821 tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac
    41881 agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg
    41941 agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta
    42001 tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg
    42061 ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt
    42121 tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag
    42181 gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc
    42241 gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc
    42301 catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca
    42361 tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt
    42421 ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat
    42481 taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag
    42541 gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac
    42601 gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga
    42661 atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta
    42721 caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt
    42781 cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg
    42841 aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca
    42901 ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt
    42961 tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa
    43021 tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat
    43081 caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag
    43141 cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg
    43201 tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag
    43261 ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt
    43321 cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc
    43381 ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa
    43441 cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct
    43501 cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta
    43561 ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac
    43621 actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt
    43681 ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata
    43741 ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag
    43801 cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc
    43861 ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg
    43921 attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc
    43981 agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca
    44041 taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac
    44101 accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact
    44161 gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt
    44221 atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga
    44281 tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac
    44341 tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat
    44401 gatagagaag actcattctt tcgtttataa aggatatata tatacattgg gagggatcta
    44461 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
    44521 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
    44581 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
    44641 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
    44701 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
    44761 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
    44821 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
    44881 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
    44941 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
    45001 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
    45061 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
    45121 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
    45181 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
    45241 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
    45301 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
    45361 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
    45421 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
    45481 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
    45541 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
    45601 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
    45661 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
    45721 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
    45781 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
    45841 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
    45901 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
    45961 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
    46021 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
    46081 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
    46141 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
    46201 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
    46261 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
    46321 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
    46381 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
    46441 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
    46501 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
    46561 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
    46621 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
    46681 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
    46741 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
    46801 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
    46861 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
    46921 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
    46981 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
    47041 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
    47101 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
    47161 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
    47221 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
    47281 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
    47341 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
    47401 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
    47461 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
    47521 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
    47581 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
    47641 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
    47701 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
    47761 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
    47821 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
    47881 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
    47941 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
    48001 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
    48061 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
    48121 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
    48181 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
    48241 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
    48301 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
    48361 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
    48421 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
    48481 gcgccatttg tttctttcaa ttgtgcggat tatgatcaaa atccgcaact gctctttgga
    48541 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
    48601 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
    48661 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
    48721 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
    48781 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
    48841 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
    48901 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
    48961 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
    49021 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
    49081 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
    49141 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
    49201 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
    49261 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
    49321 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
    49381 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
    49441 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
    49501 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
    49561 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
    49621 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
    49681 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
    49741 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
    49801 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
    49861 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
    49921 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
    49981 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
    50041 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
    50101 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
    50161 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
    50221 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
    50281 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
    50341 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
    50401 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
    50461 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
    50521 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
    50581 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
    50641 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
    50701 attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa
    50761 tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag
    50821 ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat
    50881 ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt
    50941 ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga
    51001 aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga
    51061 atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt
    51121 tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg
    51181 catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta
    51241 tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta
    51301 atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc
    51361 ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg
    51421 catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca
    51481 ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca
    51541 aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga
    51601 acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta
    51661 tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat
    51721 acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg
    51781 cttttatcgg catactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa
    51841 ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca
    51901 aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta
    51961 cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt
    52021 atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt
    52081 cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg
    52141 caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat
    52201 ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg
    52261 gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc
    52321 ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg
    52381 ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga
    52441 tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg
    52501 gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggat
    52561 caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg
    52621 atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg
    52681 aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg
    52741 gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt
    52801 atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga
    52861 tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag
    52921 tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc
    52981 agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt
    53041 ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat
    53101 ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg
    53161 aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg
    53221 attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat
    53281 gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca
    53341 agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct
    53401 tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg
    53461 atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg
    53521 tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt
    53581 ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag
    53641 aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata
    53701 aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa
    53761 aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa
    53821 aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg
    53881 aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca
    53941 cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa
    54001 tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt
    54061 attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa
    54121 tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg
    54181 cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga
    54241 tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac
    54301 acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca
    54361 agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat
    54421 tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga
    54481 aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc
    54541 ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact
    54601 aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt
    54661 tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc
    54721 tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca
    54781 gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc
    54841 aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct
    54901 tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta
    54961 cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc
    55021 ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa
    55081 gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg
    55141 aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa
    55201 actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa
    55261 agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt
    55321 aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg
    55381 tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt
    55441 taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt
    55501 tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa
    55561 tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct
    55621 acattatttc attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc
    55681 taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca
    55741 attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga
    55801 taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc ctttgcggta
    55861 caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat ttttatataa
    55921 actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc gaacaatatg
    55981 aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag
    56041 tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc catgcaaagt
    56101 aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc tgacggcgtt
    56161 tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt ttttgatttc
    56221 aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat caatcgtatg
    56281 tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc attcatttaa
    56341 tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga tatcttccca
    56401 tgttaatcct agaagttcag caaatcgcat tccagtggca ccagcaacta acacgagcat
    56461 aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag atttaaattg
    56521 atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt tgattactgc
    56581 ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca acgacgctct
    56641 aatatggtta ttaaacttaa taacggtaga ttttacatgg tctttagcat acttatttaa
    56701 aaagcgttga tatcccactc tatctaaatc agaaatcaat accgctggca tatatttctt
    56761 tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg ttccttcttt
    56821 ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc gatccccttt
    56881 aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt ctccttttgt
    56941 cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt atgaaatttc
    57001 gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcag ctctctttct
    57061 ctgatacaat aggaactata aagaagccca ttgtttgggt tttattattt tttgcacatc
    57121 ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt taatggtata attggatgtg
    57181 tattagatca tccaatcaga ggtgtttatt atgattaaag tgatttttaa aagaataact
    57241 gaacttccag agtttagatt atttgttgat tattggtatg tctggttttt tattgttgca
    57301 ttgctgatag gtctagatgt catattagaa agaaaactta agtaagagtt gagttaataa
    57361 attgaattcc taaatgagat attttgtagc ccatattttg tttgccgaca tcacctaata
    57421 ctatggagct aatcgttttt tctaacagac caaaatcaga taatctattt aagctaccat
    57481 ctattagctc ttgtgaatag ccagacatgc tgatatttcc agtgaaattt tcgtttccac
    57541 gactcattaa gcatgctaac aattgaatct caatcgatgt taattgattc agtacaagaa
    57601 gataatactc ctcgttatcc catgaagcgt tgttaattcg aagtaaacaa ttcttataaa
    57661 gatttttata catatctatt ttattttgtg cctttgattt ttctacctcg tcatttatcc
    57721 cttcgaatat tccaatgagt tggtccttgt tctctaagtt gaaaacatct tctggaattt
    57781 gatcgcgaat gttttctaat gattcgttta aatttttgta gaatttctcg atgcgtttga
    57841 aacgcttctc gtttttagac ccaaaataaa gtgactgcac aacgccacca atataaggaa
    57901 ttgctccaat accgccttca actattaatt caagatattc ttttttggtt agtttttcat
    57961 tgctattatc catagttacc tcctattttg tcatttcctt tacaagcatt gtgactcctg
    58021 atggcactct tttgtatagg ttttattatt gtgcacaatc taactcttag tggggcgtgg
    58081 ttcatttatt ttttacttac tttactatta atcatatccc attgagcata ggatttttct
    58141 atgttcgatc ctcttgaact aatttctcct gtaaaagtta ctttatcgcc ctttgtaaag
    58201 gtatcagcat tgtttaagtc cttcgcaaaa attacatatg aaactttagg tgttgcagaa
    58261 atatcattcc aacttttatc atcgtactct ttgtctgcta taatggctat tctagtagtc
    58321 attgattcga taacagttcc ttcaaaagta tatgttttac cactaatata tttagtaaaa
    58381 gctgtatttt gctcagtagc tggagtaata gaataataac tatctaagaa agcaccccag
    58441 tcatgattat ttgtctctaa aaattcttca aattgagtta ggcttttatt gctgctgtta
    58501 gatgtagtag tttgagtatc agatgatgag ctttcctgtt tgttattgtc atttgcctcg
    58561 tttgatgagg aaagcgagaa acctacggta gtaaaaataa tcccgataat aaggagaatc
    58621 ataggtgttt tcttatttgt ttttttgata aatcttacaa ttaacatgat taaacctata
    58681 ataaataaaa caataccagt aaaaccaaat ataccgtcca ttaatagatc ctctttctct
    58741 tttgatattg aatattataa accaagcaat tcttttttct taaaatcgaa ttcttcttga
    58801 ttaataatcc cttcatctaa caactcttta tactttttaa tttcatctgc acttgaaatt
    58861 tgtgtttctt tggaattatt atctaatata gattgttgct tctctatata tttttttaaa
    58921 ttttccattt cattccaata tttttttgta aacataacgg tgttttcatc tttcgtggcc
    58981 gctaatatac ctccgcgact ttcgtttccg cctagcaagg taaattgtat ataaccatta
    59041 ctcatacctg gtttttttaa ttgtacggct gatatatttt taaaggggat agttttttct
    59101 cctttaatac cttgattaac taaatttata aatccttttc taataattga aatagaatca
    59161 tcagttactt taatcaaggt ttttcctgga gattttatta aaatctcttt ttccattttt
    59221 tcttcctcat ttctgtgata tgattttttt tgtagaagct catatatgag gaaagagccc
    59281 gtgttgcagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga
    59341 tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca
    59401 gcataacaag tgtatctgag cttattctga atagaataat actccataaa tttttcgaga
    59461 ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa aattagaata
    59521 gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa
    59581 actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct
    59641 tctgcagttt caccgtatag attttcattc aaaaaaatga ttctgggttt tggatagtag
    59701 aaaccaggct ctttcatctc catataaact actttcaaat tgtattcgct caacatttct
    59761 ttcaatttca aatacataca aaccatcact ccaactattc attttcctct aaagctttag
    59821 caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa
    59881 cagtatcgtc ttctgataaa tccacatgtt taggggtttc tctttcttct ctacctagaa
    59941 ggtagtctac agagacatcg aaatagtcag caatccgact taattcatct gaattaggag
    60001 tattattttt ccactttgct agataaccgt tagaatatcc aaaattaatt tctaactgcc
    60061 gtatagatat ctttttcctc ttcgccaagt cttttattat ttcataggta ttcatcgata
    60121 aaacaacctt tctaaatgtt tacaaaaaaa gtttagaaaa atacgcagaa ataatttgac
    60181 taattctgag taatgagcta tactatatct tgtaaacaga tttaacaact aaaaagacaa
    60241 caaaaaacac tattgatatg taaatgcaga ccgccaagaa agctttaaaa tcaatgtttt
    60301 tatgtcttat ttaattatgc tcttagtata gagtattact cagatactgt caattgaatt
    60361 tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa aggaatgaga aatatgaaca
    60421 caccgcaaat ttttaatttc gaacaaaacg aagttcggac aattttagta aatgatgaac
    60481 catattttgt aggcaaagac gttgcgagtg ttttgggtta ttcaaacact aaagatgctt
    60541 tgtcgcggca tgtagatttg gaagataaga tggggtcgcg aatcacgacc tcaggtcaat
    60601 caagagagat gacaatcatc aacgaatctg gtttgtacag tttaatctta aaatcaaaac
    60661 ttccctctgc caaaaaattt aaacgttggg taacaagtga agtgttgcca gcaattagaa
    60721 aacatggagg ttatctaact ccagaaaaag tagaagaagc tttgcttaat ccagatacaa
    60781 tcattcaatt agcaactcaa ctaaaagaag aaagaactgg aagattaatc gcagaacaaa
    60841 agattgcaga gtacgaacct aaaatctcct atttagatag catattatct tctacagatt
    60901 cagtaacaat tagtcagatt gcagcagatt atgggatgtc tccacaacag atgaataaat
    60961 tacttcataa actaggtatt cagaaaaaag tcggtaacca atggttattg tgcaaaaaac
    61021 acatgaacca aggatacaca aaatctcata caactgagat cccgaaagcc gatggtggca
    61081 ctaaaattgt aatgaatacc aaatggacac agaaagggcg tctatttatc tacgaattac
    61141 taaaaaaaga aggatattac cctcaaatgg atttagagga aattggttag aaaggagttt
    61201 tagtatgact gacattgcag aaatcactca acgagataga gaaaaaatca aagaatatgt
    61261 cgaaagttca aagttcttaa cttacaccat gcttgctgaa agatttggaa ttagcaaaag
    61321 ctacttatct ttaattttaa acggtaaaaa gacttctgca gaagcaaaca gaattataga
    61381 ttcgattatc actatgtacg aattgtaaag ggaggaacag ctaatgcaat atctagaagc
    61441 aaaaatccca attccagaag gctatgtaat tatctcccaa gtggattatg aggagttaaa
    61501 aaaagctgat gatactggta gatggatgac gttgccagaa gtgctagaac ggattaacag
    61561 aaaatatgat tggtttactt ctagagtttt aaagaaccca agatatagaa atattatcga
    61621 tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt gaaggaagag acacatatct
    61681 atttttaaga agtaaaacac ttgaattttt agaaacaaat ttttcggaaa tcttaaggag
    61741 gcaagcggat gggaaaattt aatagagcgc tagtgttcag cgcaccgcta atcatctacg
    61801 ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac atggtttgga
    61861 tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca accgaggggg
    61921 ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac tcagtttaat
    61981 tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat tttgtggatt
    62041 tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt attaaaatta
    62101 attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg aatatttagc
    62161 ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc aattaatttc
    62221 ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc taaattaact
    62281 aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat catatctgca
    62341 gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt tatttttatc
    62401 cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa aattgttaag
    62461 tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attata
//
LOCUS       DAZKOM010000007        57340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000007 DAZKOM010000000
VERSION     DAZKOM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.007"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(234..2126)
                     /locus_tag="ACG8NF_000448"
     CDS             complement(234..2126)
                     /locus_tag="ACG8NF_000448"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470961.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2123..3850)
                     /locus_tag="ACG8NF_000449"
     CDS             complement(2123..3850)
                     /locus_tag="ACG8NF_000449"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0470962.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3866..4516)
                     /locus_tag="ACG8NF_000450"
     CDS             complement(3866..4516)
                     /locus_tag="ACG8NF_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0470963.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4720..5016
                     /locus_tag="ACG8NF_000451"
     CDS             4720..5016
                     /locus_tag="ACG8NF_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0470964.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5188..5808
                     /locus_tag="ACG8NF_000452"
     CDS             5188..5808
                     /locus_tag="ACG8NF_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0470965.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5900..7066)
                     /locus_tag="ACG8NF_000453"
     CDS             complement(5900..7066)
                     /locus_tag="ACG8NF_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0470966.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7078..7431)
                     /locus_tag="ACG8NF_000454"
     CDS             complement(7078..7431)
                     /locus_tag="ACG8NF_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0470967.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7559..8581
                     /locus_tag="ACG8NF_000455"
     CDS             7559..8581
                     /locus_tag="ACG8NF_000455"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0470968.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8600..8761
                     /locus_tag="ACG8NF_000456"
     CDS             8600..8761
                     /locus_tag="ACG8NF_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470969.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9020..9454)
                     /locus_tag="ACG8NF_000457"
     CDS             complement(9020..9454)
                     /locus_tag="ACG8NF_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470970.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9564..11423)
                     /locus_tag="ACG8NF_000458"
     CDS             complement(9564..11423)
                     /locus_tag="ACG8NF_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0470971.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11424..11564
                     /locus_tag="ACG8NF_000459"
     CDS             11424..11564
                     /locus_tag="ACG8NF_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0470972.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11541..12701)
                     /locus_tag="ACG8NF_000460"
     CDS             complement(11541..12701)
                     /locus_tag="ACG8NF_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0470973.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13008..14432)
                     /gene="araA"
                     /locus_tag="ACG8NF_000461"
     CDS             complement(13008..14432)
                     /gene="araA"
                     /locus_tag="ACG8NF_000461"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0470974.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14448..15146)
                     /locus_tag="ACG8NF_000462"
     CDS             complement(14448..15146)
                     /locus_tag="ACG8NF_000462"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0470975.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15103..16764)
                     /locus_tag="ACG8NF_000463"
     CDS             complement(15103..16764)
                     /locus_tag="ACG8NF_000463"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0470976.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16982..18382
                     /locus_tag="ACG8NF_000464"
     CDS             16982..18382
                     /locus_tag="ACG8NF_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0470977.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18634..19713)
                     /locus_tag="ACG8NF_000465"
     CDS             complement(18634..19713)
                     /locus_tag="ACG8NF_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0470978.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19799..20758)
                     /locus_tag="ACG8NF_000466"
     CDS             complement(19799..20758)
                     /locus_tag="ACG8NF_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0470979.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20770..22281)
                     /locus_tag="ACG8NF_000467"
     CDS             complement(20770..22281)
                     /locus_tag="ACG8NF_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0470980.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22303..22866)
                     /locus_tag="ACG8NF_000468"
     CDS             complement(22303..22866)
                     /locus_tag="ACG8NF_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0470981.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22872..23735)
                     /locus_tag="ACG8NF_000469"
     CDS             complement(22872..23735)
                     /locus_tag="ACG8NF_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0470982.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23749..24645)
                     /locus_tag="ACG8NF_000470"
     CDS             complement(23749..24645)
                     /locus_tag="ACG8NF_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0470983.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24820..26109)
                     /locus_tag="ACG8NF_000471"
     CDS             complement(24820..26109)
                     /locus_tag="ACG8NF_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0470984.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26081..27598)
                     /locus_tag="ACG8NF_000472"
     CDS             complement(26081..27598)
                     /locus_tag="ACG8NF_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0470985.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27796..28686
                     /locus_tag="ACG8NF_000473"
     CDS             27796..28686
                     /locus_tag="ACG8NF_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0470986.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28759..29583)
                     /gene="nadE"
                     /locus_tag="ACG8NF_000474"
     CDS             complement(28759..29583)
                     /gene="nadE"
                     /locus_tag="ACG8NF_000474"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0470987.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29595..31067)
                     /locus_tag="ACG8NF_000475"
     CDS             complement(29595..31067)
                     /locus_tag="ACG8NF_000475"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0470988.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31704..32066)
                     /locus_tag="ACG8NF_000476"
     CDS             complement(31704..32066)
                     /locus_tag="ACG8NF_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0470989.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32289..33041
                     /locus_tag="ACG8NF_000477"
     CDS             32289..33041
                     /locus_tag="ACG8NF_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0470990.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33181..34104)
                     /locus_tag="ACG8NF_000478"
     CDS             complement(33181..34104)
                     /locus_tag="ACG8NF_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0470991.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34119..34391)
                     /locus_tag="ACG8NF_000479"
     CDS             complement(34119..34391)
                     /locus_tag="ACG8NF_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0470992.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34861..36486)
                     /gene="groL"
                     /locus_tag="ACG8NF_000480"
     CDS             complement(34861..36486)
                     /gene="groL"
                     /locus_tag="ACG8NF_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0470993.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36537..36821)
                     /gene="groES"
                     /locus_tag="ACG8NF_000481"
     CDS             complement(36537..36821)
                     /gene="groES"
                     /locus_tag="ACG8NF_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0470994.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            37046..37708
                     /locus_tag="ACG8NF_000482"
     CDS             37046..37708
                     /locus_tag="ACG8NF_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0470995.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37784..39076)
                     /locus_tag="ACG8NF_000483"
     CDS             complement(37784..39076)
                     /locus_tag="ACG8NF_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0470996.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39246..39875
                     /locus_tag="ACG8NF_000484"
     CDS             39246..39875
                     /locus_tag="ACG8NF_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0470997.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39978..40787)
                     /locus_tag="ACG8NF_000485"
     CDS             complement(39978..40787)
                     /locus_tag="ACG8NF_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0470998.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40842..41711)
                     /locus_tag="ACG8NF_000486"
     CDS             complement(40842..41711)
                     /locus_tag="ACG8NF_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0470999.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41712..43028)
                     /gene="yycH"
                     /locus_tag="ACG8NF_000487"
     CDS             complement(41712..43028)
                     /gene="yycH"
                     /locus_tag="ACG8NF_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0471000.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(43025..44860)
                     /gene="walK"
                     /locus_tag="ACG8NF_000488"
     CDS             complement(43025..44860)
                     /gene="walK"
                     /locus_tag="ACG8NF_000488"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0471001.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44865..45569)
                     /gene="yycF"
                     /locus_tag="ACG8NF_000489"
     CDS             complement(44865..45569)
                     /gene="yycF"
                     /locus_tag="ACG8NF_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0471002.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45759..46619)
                     /locus_tag="ACG8NF_000490"
     CDS             complement(45759..46619)
                     /locus_tag="ACG8NF_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0471003.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46606..47175)
                     /locus_tag="ACG8NF_000491"
     CDS             complement(46606..47175)
                     /locus_tag="ACG8NF_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0471004.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47556..47628
                     /locus_tag="ACG8NF_000492"
     tRNA            47556..47628
                     /locus_tag="ACG8NF_000492"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47589..47591,aa:Lys,seq:ctt)
     gene            complement(48124..48510)
                     /locus_tag="ACG8NF_000493"
     CDS             complement(48124..48510)
                     /locus_tag="ACG8NF_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0471005.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(48507..49028)
                     /locus_tag="ACG8NF_000494"
     CDS             complement(48507..49028)
                     /locus_tag="ACG8NF_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0471006.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(49030..50064)
                     /gene="sppA"
                     /locus_tag="ACG8NF_000495"
     CDS             complement(49030..50064)
                     /gene="sppA"
                     /locus_tag="ACG8NF_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0471007.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(50097..51512)
                     /locus_tag="ACG8NF_000496"
     CDS             complement(50097..51512)
                     /locus_tag="ACG8NF_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0471008.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(51481..53634)
                     /locus_tag="ACG8NF_000497"
     CDS             complement(51481..53634)
                     /locus_tag="ACG8NF_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0471009.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            53892..54128
                     /locus_tag="ACG8NF_000498"
     CDS             53892..54128
                     /locus_tag="ACG8NF_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0471010.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            54147..54362
                     /locus_tag="ACG8NF_000499"
     CDS             54147..54362
                     /locus_tag="ACG8NF_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0471011.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(55065..55817)
                     /locus_tag="ACG8NF_000500"
     CDS             complement(55065..55817)
                     /locus_tag="ACG8NF_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0471012.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(55832..57079)
                     /locus_tag="ACG8NF_000501"
     CDS             complement(55832..57079)
                     /locus_tag="ACG8NF_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0471013.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(57155..57301)
                     /locus_tag="ACG8NF_000502"
     CDS             complement(57155..57301)
                     /locus_tag="ACG8NF_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0471014.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT        17043 a        11726 c         9562 g        19009 t
ORIGIN      
        1 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtaaaat
       61 cagcggtttg acctgagtat tagagataca aaacggaaaa atagcttctc atattttgcc
      121 gttttttgag ctaataccga aggacagctg attgaacacc gtttattcgg aaaaagggac
      181 tgtgacacga cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa
      241 gcttcttctt cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc
      301 aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct
      361 gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg
      421 cggttcatcg ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca
      481 tcaaggatca ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc
      541 tgcccttgcg aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat
      601 ttacgaacaa aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt
      661 gcatcgtcgt tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct
      721 tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca
      781 ccatcgtaac gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt
      841 gtcgtcttcc ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc
      901 aagttaaaat cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa
      961 cgaactttgt atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct
     1021 tcttcatcca acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc
     1081 atcaaacttg caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct
     1141 tgaacatccc ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg
     1201 aacacatagc ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat
     1261 tgagctttcc aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct
     1321 ttttcagtat gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg
     1381 ttattcaata aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct
     1441 acgatcatca cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc
     1501 cagctgattg aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat
     1561 tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac
     1621 atgatatccc catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct
     1681 tccaaatcat gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc
     1741 tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc
     1801 gcgattttat caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga
     1861 gcgccagcca ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt
     1921 tggatttgga agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata
     1981 cgtttagaca tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca
     2041 ccttttacac cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga
     2101 gagggacgtg tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga
     2161 tttcttgata agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt
     2221 ttccttcatc tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa
     2281 ctaaaacggt gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg
     2341 ttttgaaatc caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta
     2401 atgcccgtgc aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt
     2461 ccacatggct atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt
     2521 ctaatgcttt ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc
     2581 ggatcgttcc agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat
     2641 tatattgagt catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt
     2701 catataatcg agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga
     2761 ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg
     2821 ctccatgata tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag
     2881 aaactgtttt aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa
     2941 tacggtcagc tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa
     3001 agctgatcaa gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag
     3061 tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga
     3121 tcaatgtcat cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg
     3181 tataatcttt attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc
     3241 gaatcactct gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct
     3301 tttgcatact tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca
     3361 ttactgggat gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta
     3421 tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa
     3481 ccatttggat ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt
     3541 tatcaaacgc atcttttgtc agactctctg attttttata gacatccgta cgcaattgct
     3601 tacccagttt ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac
     3661 tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt
     3721 gcgctacccc tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta
     3781 cttggatcac catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga
     3841 tttttagcat ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt
     3901 tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc
     3961 gatacgaatg cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca
     4021 attcatgatc attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg
     4081 catcttgttt gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag
     4141 cgacttttct tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat
     4201 tctcgccttc caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata
     4261 gatcaccatc ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg
     4321 tctcaaagta ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc
     4381 tagggatatc cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag
     4441 aaaactcgat tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa
     4501 atgtctgtgt cggcatatgg atcctccttt ctttcaagat gacacattgt catatgacgc
     4561 tgtgtcatta taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat
     4621 catccgatac attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc
     4681 ccattaaaat agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc
     4741 caagacttca ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc
     4801 ttagattaca caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca
     4861 agacaattaa aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg
     4921 cgatcagaag cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt
     4981 ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga
     5041 ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta
     5101 ttcttcaacc ttatcaatat gacttgatag ccttttcttt ttctcatagt tataacaatc
     5161 atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa aggtcatccc
     5221 ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac aagttataaa
     5281 gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga tattccggaa
     5341 atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga attcacttcc
     5401 ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga tcagttcctt
     5461 gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc aacaaaatta
     5521 aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac agaaaacggg
     5581 ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg cggtgtatac
     5641 aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt aggcatcgat
     5701 caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc agatgagttg
     5761 atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa attgagataa
     5821 atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtacc ttggatattt
     5881 tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct tcataaagag
     5941 aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct gctgcataaa
     6001 caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc agcagaacta
     6061 ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga gagatcaagc
     6121 gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc atgaatggac
     6181 ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct aagtaaggga
     6241 tcaggttcaa aaaacccgca ataatcccca acgtgacagc atactctaaa ccaatgaccg
     6301 caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt cctcttatat
     6361 aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt tgcatgcgtg
     6421 tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat agataaaata
     6481 agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg acaaaacttc
     6541 caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc gaattcatga
     6601 acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa
     6661 tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg atgaagctca
     6721 ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt agtgcaacaa
     6781 ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt tcaaagtaat
     6841 caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc agacctacga
     6901 ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga
     6961 tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt aaaaaccagc
     7021 gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt cctcctgtta
     7081 gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat gaagatagat
     7141 tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata cgtgagctac
     7201 tgtctctaaa ttcccattgg ccatcggacc gacataatcg atcgtatata cctgatcatc
     7261 aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata cttcttgaat
     7321 caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca gcatgggttc
     7381 ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca ttgcactctc
     7441 tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg tgtggaataa
     7501 ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat cagctcatat
     7561 gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat
     7621 cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg aagaaaatag
     7681 ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta gtgtggatgg
     7741 attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg taacagagtc
     7801 tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat acggggcgaa
     7861 ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac tgaaagcagc
     7921 agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc atgcgcatgt
     7981 ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg gtacagatcg
     8041 tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta ccttcactgc
     8101 agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat tcgcttattt
     8161 atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa caggtgcttt
     8221 tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa atggaggggc
     8281 agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag gacataactc
     8341 gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc aaatcatctc
     8401 aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt ttgtcgtgtg
     8461 tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa caggattact
     8521 ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta
     8581 gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac gggaagaact
     8641 aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag aggaatatca
     8701 gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg tagaaaacta
     8761 atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt ctatcctgat
     8821 gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa aggagtattt
     8881 actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt atcataaagg
     8941 attttgatca ctctatcata aaggtaatat tacatttata ttagataact ctagttttta
     9001 ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt tggatggcag
     9061 ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat agatcttcat
     9121 ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct acatatgaaa
     9181 gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac
     9241 tgactttctt aagcattgcc cgatattttt ttgttccttc ttcaaataac aggatgatct
     9301 cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg tttctgatca
     9361 cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg atttgatcga
     9421 tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt tctccattgt
     9481 tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa aaaatttaat
     9541 ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc taaactatat
     9601 tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat ctcttgttgc
     9661 gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag ttctcgttca
     9721 aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt tgttgtctga
     9781 ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc
     9841 gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc atgcaatgtt
     9901 tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg atccccccaa
     9961 aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg cttcagtgcc
    10021 gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc acttccatct
    10081 gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat atgtgtctgc
    10141 atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt cattacttct
    10201 ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt ataggtcatc
    10261 gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc tcttttatac
    10321 atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa
    10381 acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt ggtgagttgc
    10441 gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt tgctgttcca
    10501 ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac ttcataataa
    10561 tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc tgagaaactt
    10621 tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt tttttcatcg
    10681 gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata tccttcaggt
    10741 tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc agtgttatag
    10801 tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc ttttcgcaac
    10861 aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc cacaagcagt
    10921 gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa catgtaccag
    10981 caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag catagtgccg
    11041 gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg ataaatcggt
    11101 tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat ggctacgaca
    11161 tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat aagaataatc
    11221 agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac
    11281 gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact aaaaacaaca
    11341 agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa
    11401 agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata aaagaatgtt
    11461 gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa gaattaatga
    11521 aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg gacctttcgt
    11581 actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa atcagtactg
    11641 tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat atctgcgatc
    11701 gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt tttcttttga
    11761 taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg ataattgacc
    11821 gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc caaacgtccg
    11881 ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag tatacggtca
    11941 ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg ttgtttttcc
    12001 aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt gataagtaca
    12061 gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt atcttctgtc
    12121 aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc
    12181 cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat ccctaagcta
    12241 tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc gatggttttg
    12301 ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc taaaatatta
    12361 ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga agataagagc
    12421 aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt attcatgacg
    12481 atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac accagctctt
    12541 gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact acgaataact
    12601 attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa cttttctatc
    12661 tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc tccttactaa
    12721 caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc ctagaagcaa
    12781 aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac aagttactgc
    12841 cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata aatggtgttc
    12901 cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca
    12961 atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta tctaataacc
    13021 actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt tagatttagc
    13081 gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc ttcatggaaa
    13141 ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc agtcgttggt
    13201 tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc tgccatcgat
    13261 acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc ttctctatct
    13321 cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt cggatctact
    13381 tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta agtataatct
    13441 tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc aatcgctgca
    13501 gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg ttgtacagct
    13561 agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa agcgttatag
    13621 cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac ttttacatgt
    13681 tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc cttgtactct
    13741 tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac aagatctccg
    13801 ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt gtctccttct
    13861 gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc aaaactttca
    13921 ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc aggtcgtttc
    13981 cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa cccatattcg
    14041 cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt tttccaagga
    14101 atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag ctttgtcccg
    14161 cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc tgctacttct
    14221 tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac tgctaattct
    14281 tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac catttcttgt
    14341 gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga tcctacaaca
    14401 aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta tttgttttgt
    14461 ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat ccgttgaggc
    14521 agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca tacttcatct
    14581 agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa tggaccgtgt
    14641 ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt gaatgtttct
    14701 atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg tgtcaaaaat
    14761 cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat cgcaggaaca
    14821 tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac gattcctccg
    14881 atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga gggattgagt
    14941 ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc tgtcatctct
    15001 tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc aaaagcgctc
    15061 acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt tgcttggtat
    15121 acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca attgatgaat
    15181 cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct gatagcctgc
    15241 tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca cgtcttctgc
    15301 caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta ataacgcgat
    15361 tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct ccatagcaga
    15421 tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc ccactaaacg
    15481 atcaatttgg acattctcag atttcaatat atccattccg attctcattg caccaaaagc
    15541 actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg gtgtacgtac
    15601 aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat aaccataaga
    15661 aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata ccgtgaataa
    15721 tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa tactgaccca
    15781 agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt ctcctgaagg
    15841 tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta atacgatcat
    15901 agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccgt ttggctacac tgttcgtagc
    15961 gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg gtctcaaatt
    16021 tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt cttctcctgc
    16081 tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt tttcgattaa
    16141 ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat cgataggaaa
    16201 catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc aatggacata
    16261 acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat tcaaaataga
    16321 ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt tttccgttaa
    16381 tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta ctagtaactg
    16441 ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa tcgacccgat
    16501 tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc ggtaactgac
    16561 gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt ctagtttgtt
    16621 ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca atacagcttt
    16681 gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga catctaacat
    16741 actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc aaacctgatc
    16801 aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg tacatgtcaa
    16861 gcggttattt acaagaatac aataaaaaac acttgttttg tacgtacata agttataaaa
    16921 tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg agagaatact
    16981 tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg catttggcgg
    17041 cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt tacagcatga
    17101 ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg tcatgttagg
    17161 tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac gacgaaaaat
    17221 gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat ctgcccctca
    17281 caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg ttggcgctgc
    17341 atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa ggggaagatt
    17401 gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg ttgcagatta
    17461 tttgctaaaa gtgttgcctg aaacgatggc ttggcgcgtg atgttagggc tcgctgctgt
    17521 ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc gttttcttat
    17581 gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga caccaaaaga
    17641 agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga caaccggaac
    17701 ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg gtattggtgt
    17761 agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc cgctgatcgt
    17821 agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca tccaaggaat
    17881 cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca atcggcgaac
    17941 tttattgaaa ataggcggta gcgtgatggg actttcattc atcctaccag ctgttttagg
    18001 aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat gtatttatgt
    18061 cgcattttat tcttgcacat gggctccact gacatgggta atcataggcg aaatattccc
    18121 actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga ttggttcttt
    18181 cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa tcgttttcgg
    18241 catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg taccagaaac
    18301 tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt atcctaaacg
    18361 tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc aagaaataag
    18421 aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
    18481 atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga gaggtggtga
    18541 aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga cgagtaaccg
    18601 taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt tcactgtttc
    18661 ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt tttcaatcgt
    18721 tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg tcaatgttgt
    18781 caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga tcgataggtc
    18841 atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag ctatttgatc
    18901 gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta ctgttttttc
    18961 agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca aaaaacgcac
    19021 attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt gaagatcgtc
    19081 gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt ctgctgccat
    19141 aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt cataagcagc
    19201 attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg ctaagtttgg
    19261 gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat aagcaatcat
    19321 tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta aaagcaatga
    19381 atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa aaatataatc
    19441 agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg ttttttcttt
    19501 cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat agccctcatt
    19561 taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata tttcttgcag
    19621 cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga tcttttgttt
    19681 tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt atttgtacgt
    19741 acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt tcttgtgctc
    19801 ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc cacgtaaagc
    19861 attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg ttcttctctg
    19921 gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta aacgagttgt
    19981 gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc gataatttat
    20041 gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg cccatggcat
    20101 agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg cgagtaagga
    20161 ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca ggtatagaaa
    20221 ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga taaagattag
    20281 aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt ccttgagaag
    20341 agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt cccttttcta
    20401 tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg cggtgatgat
    20461 gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac cattttccat
    20521 tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct tcatgagcct
    20581 gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc cttcttaatt
    20641 caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct gtatgtttat
    20701 atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc ttcatcataa
    20761 caaatttcct cacattttct ttattctaat tacattccaa gagtattttt ctatccctac
    20821 atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta ttttttctgg
    20881 ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt gctctccttg
    20941 atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg tgcgattgat
    21001 caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat aaggaacatt
    21061 ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata cttgaccttt
    21121 agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg cttgtccttt
    21181 agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac taatctttac
    21241 gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg tatcttccat
    21301 agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc ttttttccgg
    21361 ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac ttctcaaata
    21421 tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga atcgataagc
    21481 ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag tcaagtcttc
    21541 ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct cataagcttc
    21601 ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag agcttccaac
    21661 taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt catttgctaa
    21721 cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta gctcattccc
    21781 taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc ttaaatcact
    21841 ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt catcaattcc
    21901 ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga tccatttaaa
    21961 aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat caattttttt
    22021 tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg taaagtttcc
    22081 tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga catcttgtct
    22141 aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat agacgacact
    22201 tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta ccgtaaaatc
    22261 ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt catttcctat
    22321 agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa taaaaggagg
    22381 gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa tatttctcaa
    22441 aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg tgatatcttt
    22501 gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa gtaaaactac
    22561 atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa caagaaaata
    22621 aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct ttattccttg
    22681 aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca ttacataaaa
    22741 aacagcacat gtagcaggca cgatagtaat aacaggcaaa cacacgatta cccagagaat
    22801 acctaataag atgtaatcag aaaccacaat caactgtcca taaaatttag tctcaactat
    22861 tttcatcttc ctcactcttt cattccagac atagctgttc cttgaacaaa atatttctgt
    22921 gcaaaaatat aaaccactag taatggcagc attgaaataa ccgttccagc cattacaggt
    22981 ccataaaaag aaatgttctg ccctgtaaat aaagctaacc ctgctggtaa agttctcatt
    23041 tttgaactac ttgtcaatat caatggataa agcaagtcat tccaactatt catcagcgtg
    23101 aaaatcccta atgataaaag agtaggtttg ctcaatggca acatgatttt caaaaaaata
    23161 ttaaactcac ttaatccatc aatgcgtgct gcctcttcca aagaatctgg taaagtaata
    23221 aaaaaggaac gcatcatgaa gataccaaat gcagttgtca tacgaggaat gatcaaacct
    23281 gcataagtat caagcatgcc aagcaaattg acttcaataa atagaggaat catgaagact
    23341 tggaatggga tcatcattgt cagcaaaacg aaatagaaca aaactgattt tcctttgaaa
    23401 cgcatacgag caaatgcata ccccgcaaga gaatcaaaga atacagaagt gatcactgtc
    23461 cctccagcaa aaatcactgt atttttgatg taatctaata aagggatcgt tgtccaaacc
    23521 ttaatatagt tatctagggt atagctatct gcaaatattc taggcggaaa tgagataatg
    23581 tctttttcgt gtttaaaaga agaaataatc agccacaaaa aaggaaacgc aataaccaac
    23641 aatataacta gcatactaat cgttcgaatg atttttttac cactaactgc ttttcgacta
    23701 ttcacagtaa ctgtaatcga gctttctggt gccatacagg tttccctcct atttcatttg
    23761 tttttctttc cgtgacatgt acacattagt ggagatagct atagttgcaa caatgataaa
    23821 caaaaccgaa gacagtgctg atgcatagcc caattcataa ggtgctgtaa atcctcgact
    23881 gtaaatatat tgaacagctg tttctgtctt aaattgtggg ccaccagcgg ttgcaacata
    23941 tacttggtca aacacttgca tcgaaccaat cagattcgtt aaaatacaaa aacctaagga
    24001 gggaacgatc tgaggtagcg taatgttaaa aaactgctgt cgatttgttg caccatccat
    24061 ttgagcagat tcatacaaag agggagagat tccctgaata gcagtcaaga gaatcgtcat
    24121 agtaacacca aaattcttcc aaacagtcat gatggctacc gtcggcatag caagggttgg
    24181 atcgcgaaaa aattgaggga tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc
    24241 tacattcgga tcaagcaaca tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac
    24301 tggaatgtaa aatgtcgaac gacaaaaacg attgaatctg gtatttttag ataatgctac
    24361 tgctgctaat aaaccaacga taatttgtgt tggcgtttcg agtacggtga aatataaaga
    24421 atgaatcaac gaattaactg ctcgttcatc actaaatata cgtgtgaaat tttcaaaact
    24481 tacaaaattt cttgatgtaa agaaaatatt caaatctgta aaactaatag caaatgttcc
    24541 taaaagcggt atgacagtaa ataataataa aactaaaaaa gatggagtta aaaacaaata
    24601 cgcagcacga tcaggacgat tccaatattt ggtgaatccg ttcatatagt ccacctcatc
    24661 tttcattttg ataaaatata caatcttggt ttaaacgaat ctgaataaga tatccgaaca
    24721 atcgctgtga tttttgcaat aacacctatc tgcaaatact aggtcgatca ttcagatatc
    24781 tgtatcttgc caacagtcta tcctgtataa agttctgtac tatttttgca gtaattgatt
    24841 gatggcatca tctgcatcgt tcatgagtgt ctcaggatct tctcctgcta ataatttttc
    24901 aatcagtgga ttgatgatat cgttattgat cgcagagatt tgggtaaaac ctggtaaata
    24961 tggctctgca tagtccattt gtttcgataa ttcagataca ataggatcat tttttacttc
    25021 ttcatctttt gctactgaat ccaagtaggc aggaaaacca ttttcgatac tccatttttt
    25081 accaatttca gttgtattcc aatatttcaa aaactcatag acagcttctt ttttattatt
    25141 atctgtactt gccggaatgg catatcctac gccatctaaa atagctgttt ttttgtctga
    25201 atcactccct tgtggtaagg tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt
    25261 atttaaccat ggcccattga tttcaatggc tagttgacca gcattcatta aattgtccat
    25321 ttcggcgcca gaaatcgatt ctggaccagt cttgtctaca tggatcattt tttggatcgc
    25381 ttttaacgtt ttaaggttct caggagacgc aaattcagac ttagtataat catcgttcac
    25441 taatttaccg ccatttgcta acatccagtt atataacggt gcagttccat ccttattgaa
    25501 gacaaaacct gatacatttt tactactatc agttaatttt ggtgccattt ctgtcaattc
    25561 ttcccaagta gtaggtggtt gttcagtatc taatccagct tgtttaaaca aatcattatt
    25621 ccaatacata tacatgcctt gtacctgcat ggggacaaaa tactgtttac cttctacttc
    25681 acctaattca atagccgttt ctttaaaatc agatttatct actccatcat attcccagaa
    25741 atcgtctaat ggttgtaccg ctccgttttc tacatactgt gcaaaatctg gataatttaa
    25801 tgcaataaag tcaggtgctt ttttagaaga aatcgctgta ggaagttttt cattcaaatt
    25861 ggcccaagtc atgatatcca tttcaatttt gatgttttta tcatttgttt tattaaattc
    25921 attaacgatt tcttgcaata tttcaccatc agatgcagta aacccattcc aaaaagtcaa
    25981 agtcacttct ttatcagaat cagattctgc tgaacttccg ccaccgcaag ctcctaaaat
    26041 acctgttgca gctaccaaca aagagccaat taaccatttt ttacctttca ttttttgttc
    26101 ctccttcatc tgataaacga catctgacca cattccaaga tagcggtttc aataaacaat
    26161 tgattttccc atcttcagct tttacatttg ttgaatgact tggttttact tgttcacttt
    26221 taggaagatt agttgctttt gtatcaaatc cactcatttc agaaaaatca atcactccct
    26281 ctaatgataa ttcagacaag tgaacagtta gattcatttc ttcttctcca cggttaactg
    26341 caaatatgac aagttctttg tcatttatta cggctatact ttctaaatac ggcacttctt
    26401 caaaatcttc cgtcgcatag tttgctgaac gaatttgtgg gcttaacacc tctccttgtc
    26461 catacacagc agcttgcata aatggataat agatcgtctg tctccaagct tctccgtttg
    26521 tttctgtcct gattggtgca ataacgttta ccaattgagc aagacaagca atctttactc
    26581 tatctgcatg tttcaataaa gtgattaata gacagcctaa tagtaatgca tcttcaaaat
    26641 tatagttatc ttctagtaaa ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt
    26701 ccgctgaatg ataccaaata ttccattcat caaaggacag gttgatctgc ttcttactat
    26761 gtttcttcgc cttgactgta tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt
    26821 ctaaggaacg tgcaagataa tgagaaagat catttttttg gttgccataa tattgatgca
    26881 gagacaaata atctatttga tcgtacgctt gatcaagaac tgttaactcc caatcaccaa
    26941 atgtaggcat ttgccgattc gaacttccgc ataaaaccac ctcaattgaa tcatctacga
    27001 gtttcatcgc cttagccgtt tcctctgcta accgtccata ttcttccgct gttttatgtc
    27061 caatctgcca aggaccgtcc atttcattcc ctaaacacca tgtcttaatt ccaaaagggc
    27121 tctcttgacc attcgaacgc cttaaatcac tccaataagt tcctttagga aaattacaat
    27181 attcgactaa attacgagct gcatctactc cacgagtacc cagattgaca gccatattga
    27241 tttcggcccc cacatgctga caccagttat aaaattcatg aatcccaact tcattcgttt
    27301 caattgtccg ccaagctaaa tccaaccttc taggtctatt tgctatgggc ccaatactgt
    27361 cttcccaacg aaatcctgac acaaaatttc caccaggata tctaatcatg ggaatgttca
    27421 attcttttac caagtgaatg acgtcttggc gaaatccact tttattagct gatggatgat
    27481 ctggctgata aatcccttca tatactgctc gtcccatatg ttcaataaat gaactgtaca
    27541 accgcggatc aactttgcct acacgcttga cactactaat caacattgtg gcttccataa
    27601 ctgacctcct ttaatttata catgaataat acacctcgtt gaaagcgttg tcaataatat
    27661 aaatgttaaa cttacttatt ttttatcgat tttaattaac caaaatatta ataatgatta
    27721 aaacaaataa tttaatataa cttttattaa caaatatgtt aatatataag aaaaaccttt
    27781 taagaggtga tatatatgca actacaaata aacgaagaat ccttaccggt atatgaagca
    27841 ttagctagta aaacaaggat caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc
    27901 aaagatttgg caaaagaact tggagtgagc agtgctatca cgacgatgca cgtgaaaaag
    27961 ctagaggaag caaacatcat caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt
    28021 cgtgtcgata aaatcgatat tagttttcca gaaaaaatat tcaatgcttt tgatacgaaa
    28081 gaaacatcga tacctattgg tcactataca aattatgcaa ttgaaccaac atgcggttta
    28141 gctacgatcc atgattttat cgggaaagtg gatgaaccac gctattttat ggatcctcga
    28201 aggatggatg cccgcattct ctggtttacc agcggatttg tcgagtatca ggcacctaat
    28261 tttttaaata cagaggatac attggagatg ttggaagttt ctgtggaaat ttcttctgaa
    28321 tttccttttt caaatgacaa ttggccttca gatatcactt tcagcctaaa tggtgtggag
    28381 ctaggaacat ggacgagccc tggagatttt gctgatatcc gcggtaaata tacgcctgat
    28441 tggtatccag ataatcttaa tcaatacggc ttgctgaaga cgatccgaat taccaaacac
    28501 ttgacgaata tgaatggaga acccttgtca aacatcacga tcaacgacat ccctaaagag
    28561 caagacactt ggcatttgcg aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt
    28621 actttatttg gtaaaggatt cggtaattac gatcaggata tcaaattaaa agtttactac
    28681 agctgatatt tttcttttct attttataaa acaaaaacag ccaatcccta tgaagggacg
    28741 gctgtttttg ctttattgtt atttccagaa atcatccaaa acggaaattg gcaaatgtcg
    28801 tttatgttgt gtcttgttcc accatgcttc gattttttct tgtgcttgag cgggaacttc
    28861 ttttccttct agataatcat caatttcatt atatgtgaca cctagtgcga cttcatctgc
    28921 aatcattggt ttcccatctt caagatctgc agtcggaatt tttgtgtata atttttccgg
    28981 tgcattcaat gcttgaagca attgtttccc ttgtcgtttg tccaaacgga ataatggcaa
    29041 aatgtcggct ccaccatcac caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg
    29101 atctgtccct aatacggctc ctgctctttc tccagctacg gcatattgcg taatcatacg
    29161 ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt cccacacctg cttcgcttaa
    29221 tacttgtact tgtgcatcaa cagctggttt gatatttact cgcagactaa catcgggctg
    29281 gataaagtca agagcagctt ttgcatcttc ttcatctgct tgttcaccat aaggaagacg
    29341 aacagcgata aattgatagt tttcatcttt tgtttcttca cgcatttctt ccatcgttag
    29401 ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg ctgataccta gtacaaaagt
    29461 tttaagaaac ggatattttt tcaaatatgc tttcataaag tcaatacttt tacgtatttc
    29521 ttcttttgga tcaattgttg gtttgacacc taattcattg ataatttctg tctgcaggct
    29581 catttattct cctcctatgc ttctgtatga agttctgcta cgcttttttt cattcgttcc
    29641 atgatagcca ttttatggtt ccagcattca gtagacagat cgactgggta tttttgcgga
    29701 ttcaagtctc gtttatactc ttcccatagt gaatctagat tttcttttgt atattctttg
    29761 atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt cgataaagat atcttgtaaa
    29821 actgggcgag ctgtaaagtc tcgaacagtt ttattgataa acgtatgcac tggatggaac
    29881 atgaagatag cttcttcttc tcgagggtct tcatcccata atgtcacgta gtctccttca
    29941 gatttcccat caaaatttcg agtgatgcgc cacacttgtt ttttccctgg agtagttact
    30001 ttttctgcat tgcttgataa cttgatcgta tccatcattt ttccttcatc atcttcgatc
    30061 gatactagct tgaatactgc accaagggct ggctgatcat aggctgtgat caattttgtc
    30121 ccaacacccc atacatcaat tttagctttt tgcatcttta aattcaaaat cgttgcttca
    30181 tccaagtcat tcgaagcata gatttttgct tccgtaaatc cggctgcatc taattgttcc
    30241 cgaacacgct tagatatata ggccatatct ccactgtcga tgcgtacgcc ttggaagttg
    30301 attttgtcac ctaattcttt cgccacgcga atcgcacttg gcacacctga tttcaacgta
    30361 tcataagtgt cgactaagaa cacgcagtct ttatgggttt tagcgtaggc cataaatgct
    30421 tcataatcat ttccataaga ttggactaac gaatgagcat gggtcccgct agctggtatc
    30481 ccaaatatct tcccagcacg tacattgctt gttgcatctg ctccgccgat atatgccgct
    30541 ctagttcccc agactgctgc atccagttct tgtgcccgtc tagttccaaa ttccaatagt
    30601 ggatcttctc cgatgacaga tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa
    30661 ttgaccatat tcaacagtgc tgtttctact aattggcagt gcgctaaagg cccttccact
    30721 tggatcaatg gttcatttgc aaaaactagt tctccttcac gagcagaacg aactgttgct
    30781 ttaaattcaa aattttgtaa atactccaaa aaaccttcag gatagacttc taatccacgc
    30841 aagtaagcaa tatcggaatc attgaacgtc agattctcta aataattgac taaccgttcc
    30901 aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt ctcgaaagta acattcaaaa
    30961 acagcatgta aatcagctcg acctaattcc cagtatgtct gcatcatatt gatttgatat
    31021 agatccgtat gcagcgtcaa actgtcgtct gggtaagtat tattcatctt gtattactcc
    31081 tcttggcaag ttttcatttc ctacccatta taacaaaaaa aattaatagg aaaagaaatc
    31141 ttccattttt tctgaaaaag cacatgtttt acggatattt cttgctaaaa atgaactcca
    31201 tctgctaaaa ctatcctttc ttattggtat ttaacctaaa cgagaaagaa tacttatttt
    31261 attcaatgta tccgagacac gaagaaatta gcgttttcta tttttcttac ttgataaaat
    31321 aagtagattt tcctactaca atcgtagccg ttaagatacc gatgattgaa caaatataac
    31381 caaatgtctc tacaacataa tcaatggcaa aagtttttga agcaataata cgtctttcct
    31441 tttttgcttc ttatccccaa agaaacggat ataacttatt atgtattgaa tttttttgga
    31501 aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac
    31561 gtttttaaaa agatacaaag taagaaagca gctgtcaata gaatggaaac aaaaatggcg
    31621 agacatcaat cagtctcgcc ttgtttttta ttgtatacat tagatatgga gcaggttacc
    31681 ataaagatga ttattagtcg ttattaaata gaatccttcg tatttttttg ttctatcact
    31741 acaatatctt tatttggacg tttgaattgt attccggcac gttctagctc ctgtttcact
    31801 cctacatgga ctttgacatc ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg
    31861 atttcttgcc ttgtgatttt tttcttcgta ttgatcatca cgacttgctt tttatcatat
    31921 gtgtctacca atagcagttg tgttcgatct aacgaaaata atttgtaata attaatagcc
    31981 gtattcccaa aaaagaattc cactacttta ggataaaatg tcttaatatt taaattacta
    32041 ttcacaggtg tttcgattag cttcatctca ttcctctttt ctatatagtc gatttagatc
    32101 caatcttaag ttatgttacc atctttccta gcattaagga atcggttaca attgaacctt
    32161 taaagacaaa aggatttacc ttttatcact taatagtata acataatttc atgagaaaaa
    32221 aatacaattt tttcttgacc atctcgttac gtaaaggatt atgcttacat ttgtcaggag
    32281 gaaaaataat ggagtacaca ataaagaaaa tgtcagagat atcaggagtc agtccgagaa
    32341 ccttgcgctt ttatgatgag attggactgc taaaaccagc taggatcaat tcttcaggtt
    32401 atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt tatcgaacaa
    32461 tggcattcaa gttggatgat atccaagagg tcttagacaa tccatctttt gaccaccaga
    32521 aagctttaat caagcatcag caaatgttgc tggaaaaacg ggcacaaatc gataccctct
    32581 tgacaactgt ccaacagaca cttgatatgt acgaaggagg aagaaaaatg agtgacaaag
    32641 aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag
    32701 agattcgaca aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata
    32761 tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca
    32821 ctctttactt aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc caagctcata
    32881 agaagtggtt atcttacagc tggcctgaat atcagccaaa agcgcataaa ggactaggaa
    32941 tgatgtacga agccgatgaa cggttcactg catattatga cgagcgaagt ggtcatggtg
    33001 cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta atcttttaca tgatccaaca
    33061 aaaaagtaca cgtaaaatga cctaataaca aaacagcaga cacccaagtt agatttttat
    33121 gctaactttt ggtgtctgct ccattctttc acaagttttt tcatcctact ttaaatatat
    33181 tcatcgttga ataaacgtat aaggtatttt cactttatcc tttttttctg taagaaacaa
    33241 ttgaaatgcc atttctccgc aagcttccag atggtggtcg atggttggga aaccaagaag
    33301 ctcgctgatc agcaaattat cgcgtccaat caccactggt gtcttatctt gcagatagct
    33361 ttgcaagatg cctgctgcaa ccatatccga atttgtaaag acaccatcaa ctccaagctc
    33421 tttaaaaaag aagcctgctt gttttccttt ctctgcatct atacaatccc aaaaaacaag
    33481 tgattcatca aaagatggaa aaagttgatg acatagctgc aaagtgattt ttgagttata
    33541 acttagcctg ccacttcgcc ccaacgtgat gcccaattgc tttatacctt gctgcttcaa
    33601 ataagtaaga ccttctgtaa ttgattgttc tcgatcaata aagacacagc ttgcctgttt
    33661 tcccttgatt tcttcacaaa aaactagagg tccatatttt tgatatgcca ataaatcatc
    33721 tagtgtattg gcacgagacg taatgatcaa tccatcgaaa gctttagctg caaattcttc
    33781 aagatacctt cgctcttttt ctttctgata gcccgtcggt aaaagtgtca ctttgtaatc
    33841 ataatcaaac gctgcttcaa tgatcccact gatcaactga tcaaaatacg gatgattggt
    33901 ataaggcaga atcactccaa tgcttttcgt ttttccataa cttaactgtc tggcatttgc
    33961 attagggata taatcgactt ctttcatgac cgcaagtatt tctttccttt tttcctcatc
    34021 tacatatgga tgtccattga ttacacgtga aatagtagaa actgaataac ctgtgagctt
    34081 tgcgatatct cgaatgtttg ccactgctgc ttcgccacct atacaaaaag attttcttgt
    34141 aaatagtcta ccacagctgg accgcattca aaaaactgat acatttttcc ttcatgcgaa
    34201 aaactgatcc gctgcccgcc ccaatatata tcttttgtca aattttcgat ttcattcatt
    34261 ggtatttctt ttactatatt cctattcaac ccttgaaaac tgtacaacgt taatacggat
    34321 cccttgaact cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt tatctctaca
    34381 taagtattca ttgcaagccc tccttttgat actttgagta tagtgtatga aacgcgtctc
    34441 atagcaagga gtttgttttg ccaaagaaaa gttttattcc tctttcatta ttaccaaagt
    34501 ctcttatttt aaattgaata cattataatg atactgtaaa tatatcttta ttattagtga
    34561 atggatattt acttttaata gataaaatat tataactatt aaaaataact attttcatta
    34621 taattacaca caaaaaagag cgtgagacaa aagtaaagaa cacttttgtc tcacgctcta
    34681 aacacgtata aacagcggaa gcagaagcaa ctccttcgga aataagctga aattcacaaa
    34741 aatttgaaaa gcaatttccg tgaatttctt cttatttctt ggagttaaac gcttctgttc
    34801 cagcctcttt ttatctgtct ttcactaatg atcagttgta gataagcaag ttgctttttc
    34861 ttacatcata ccgcccatca ttgatggatc cattgcagga gctgctggcg ctgctggttc
    34921 tggtttatct gcaacgactg cttctgtcgt taataacaag gcagaaacgg aagcggcatt
    34981 ttgcaaggca gaacgtgtta ctttagttgg gtctacgata cctgcttcaa ccatgtttac
    35041 ccattctcca gtagcagcat tgaaaccagt gcctagttcc acgttcttca atttatcaac
    35101 aatcactgat ccttcataac cagcgttttc agcgatttga cgaattggtt cttctaaagc
    35161 acgaacaacg atcttgatac ctgttgccac gtctccttct gcttctacag cagaaacttt
    35221 gctgataacg ttcactagag ctgtaccacc acctgagacc attccttctt ctactgcggc
    35281 acgtgtagca ttcaatgcat cttcaatgcg taattttaat tctttcaatt ctgtttcagt
    35341 agctgcacca actttaacaa cagctacacc acctgctaat ttagctaagc gttcttgtaa
    35401 tttttcgcgg tcaaaatcag aagttgtttc agcaatttgg tttttgatca attgcacacg
    35461 agcttcgatt gcttcttttt ctccagaacc ttctacgatc gttgtattgt ctttatctac
    35521 aacaacttta gaagcatttc ctaagttttc gattgttgca tctttcaatt ctaaacctag
    35581 atcgtcagta attactgtac cacctgtcaa aatagcgata tcttcaagca ttgctttacg
    35641 acgatcgccg aaaccaggag cttttacagc tactacgttg aatgttccac ggattttgtt
    35701 caatacaaga gttggcaatg cttcaccatc tacatcgtca gcaataatca acaatggacg
    35761 tgattgttgc aagatttgtt ctaacaatgg cagaatatct tggatgttag agattttttt
    35821 gtctgtgatc aagatatatg ggttttctag aacagcttcc attttgtcgt tatctgttac
    35881 catatattgt gataagtaac cacgatcaaa ctgcatacct tctacaacat ctagttctgt
    35941 ttcgatccct tttgattctt cgattgtgat gacaccgtcg tttccaactt tttccattgc
    36001 atcagcaatc aaatgtccaa ctttatcaga acccgaagaa acagctgcta cttgtgcaat
    36061 ggcttctttt gaatcaacaa cagttgagat attatgcaat tcttcgactg ctgcttttgt
    36121 tgccaattcg atcccgcgac gaatgcctag aggattagca cctgcagtta cgttttttaa
    36181 accttcgcga acgattgctt gtgtcagaac agttgctgtc gttgtaccgt ctcctgcaat
    36241 atcatttgtt tttgatgcta cttctgatac aagttttgca cccatgtttt caaaatgatc
    36301 ttctaattcg atttcttttg cgatcgtcac accatcattt gtgatcaatg gagagccgta
    36361 agatttttct agtacgacat ttcggccttt aggtcctaat gttactttca ctgtatctgc
    36421 taatttatct accccgcgta acatagctgc gcgtgcgtct tctgcaaatt tcaattcttt
    36481 tgccatgatt gattcacctc attgattatt tttgattatt tttttatctt ccaaatttac
    36541 tcaacaatcg ccatgatatc ttttcctgcg acgatcagat attcttttcc ttcgtatttt
    36601 acttctgtac cggcgtattt ttcgaacata acttggtcgc ctgcttttac agcggcagga
    36661 actttttcac cgttttctaa caaacggcct tcaccgactg caacaactgt cccagtttgg
    36721 ggtttctctt tagcagcaga agctaaaacg attcctccga cagttttttc ttcttctttt
    36781 gcgacttcga taatgacgcg atcacctaat ggttttaaca cgataaatcc ctccgataac
    36841 tcttttttta atctgttagc actcttaacc ttcgagtgct aacctacatt tttaataata
    36901 ctcattctcc ctcttttttg caagctttta aatcaaagaa aagaaatttt attctgacag
    36961 ctgcttattg aaattatgat ataattgaaa tcaatcgata taagcttgcc ttgcatgctt
    37021 gttatccctt aaaaggagga attacatgtc tttaaaaaaa tatagcttcc tcagcatttt
    37081 ttgctatggt ctaattttta tctcaccctt gatcctagct tcgataggac ttgtaaaatc
    37141 tacgtctgaa ttgatcacag caactgcagt catgtatata ctaggcgccg ttgtcctagc
    37201 tattttttat tttaagcaac gtgaaccctt agcgatcgaa tcagcggtaa aaaaagcttc
    37261 tgctccaaag attgttattt atggattggt tggaatcttt gtcgcattga ttctgcaaag
    37321 catcgctgtc atgttagaat ctgtattgtt tggcaaagca accccttccg agaatacaca
    37381 aaatatcatc cagatgatca tggaagcacc cgcttttatt ttggcgacaa ctgttgctgg
    37441 acccatcatg gaagagttcg tattccgccg cagtatactg gggatcatca gtcgatattc
    37501 aaatttttgg gtcggagcag tgatcagctc tttgctgttt gcgtttgctc ataatgatgg
    37561 tcatctgcta atctactttt tcctaggttt cttcttcagc ctagagtaca aagcaactgg
    37621 tcggatctgg acatcgatga tcacacacgt aggaatgaac acattagttg ttcttgttca
    37681 attagctgtc caaaaagggc tagtataaac atgactataa atactcctgt catcctagat
    37741 tggtgatttt cccaatctaa agcgacagga gtatttttgt tttttagtcg ttcatctttt
    37801 ctgctgctcc atcatttttt gtctgtcttt cctttttgca gtttgcagaa tctatttttt
    37861 tcaaaaatgg gaaataaatg aaaaaactaa ttgacaacac gataccttgt aaaatcgctg
    37921 caggcactcc gcctactaag aaacccgaga ggatcggcgg tgttgtccaa gggacttgga
    37981 ctgctgtaaa taaaggaacg aatccaaaat ataaagcgga ataggtaatc aatcctgaaa
    38041 cgaccggtgc gaaaataaaa ggaacagcca tcatcgggtt catcacgatc ggtgtggaaa
    38101 aaataattga ttcattgatg ttgaaaaagg ctggtaaaag agcaagattt cctaattctc
    38161 tgtatttctt cgatttcgca aaaaccgtca taaaaaatac agcccctaac gtcaatccag
    38221 cacccgtaac cgtcatgaac tgatcgagaa attgctgagt aacgatatgt ccgccatttg
    38281 ccaccgtcaa ctcttgtcct gaggctaaga tttctgcatt tgctaatgaa ttcgcttgca
    38341 agacgggact aaccaaccca ttgaccatcg cagatccatg aagaccaaat aaccataaaa
    38401 acggaacaaa aaaacctaat aaaagcacac caaacaacgt gtctatgaac gattgaatgg
    38461 gaagctggac atatgtccat actccttgaa caacagtcat tctgaacaaa tgttgaaaaa
    38521 ccgctgaacc gataagcgta aaagtgacta taacaatgat tggaatcaat gaagtaaaag
    38581 actgccaaac attttcaggt atcttctctc tttgcttcca ccctttcgca agtcttacta
    38641 aacgtgcata gccccatccg atgatgagcc cagttgtaac tgctcctagt atcccttcag
    38701 cacctgtcca gacagtatct atctgagtat ttagtggctg aataagcagt agataagcag
    38761 acaaggctgt tatgccgact ggtaagcgat cgatgtcttc ttcttttgca taagcgtatg
    38821 caatggcaaa aacgatccaa aaggctatca catcaaatgt agcctggtat acttgtgtaa
    38881 aatacataga aaatcctgtt ttgtctatcc actgagcgat gtaatccaca ggaaaatgag
    38941 caaataagaa aaacagcgag ccaacaataa ttattgggat cgtgtaagtc attccattac
    39001 gcaaagcagt taagggtcta agattcacaa cagatgttat cattggtaaa aaggcacgat
    39061 ctaaccactc tttcatttct tatctccttc aacttctatt tttatcaatc agttttctta
    39121 atttaactat acccaatgat cacgaaccat gaaaggaatg cgcactgagc ttttcaaaaa
    39181 tcggattctt tgacgaatat tcagcaactg cttacaatgg acaggaataa aaaaggaggt
    39241 tttttatgaa aattgcactc attggagcta ctggccatgc aggttcattt attttagacg
    39301 aagcattgtc cagagattta gacgtgacgg caatcgtgcg tcatccagaa aaattgccat
    39361 cagatgttcc gtttatacaa aaggatttat ttgagttgac aactactgat cttgcatctt
    39421 ttgatgttgt cgttgatgct ttcaacgctc ccaaaggaaa agaagaaatg catcaaactt
    39481 ctttgtccca tttgattgag atgcttgaag gaaccgatac acgcttaatt gtcgttgggg
    39541 gtgcctcttc gttattctta gatacagaaa agacgactcg tatgattgac caagtaccta
    39601 aaaatgcacc cttctatcca acagctttca atatgtacga aggattgtta aaattgaaac
    39661 aatctaaaaa tctacattgg acttatatca gtccagcttc tttatttgat ccacatggca
    39721 aacgtacagg tacttatttg ttaagtgatg attatttaca aagaaacgct gctagcgaca
    39781 gtacgatcag catggcagat tacgcgattg cattagtaga tgagatactg gatgagaaac
    39841 atgaaaagca gcacatcagt gtagtcagtc gataatacgg ttattttttt aatatgctaa
    39901 aaaaataaaa atagaagata aaaccaaccc tgattgattt tatcttctgc tttttatatt
    39961 tttattgatt gaactgttta aatcgaaaat agctcagttg cttcatctgg atctgtatca
    40021 tagatttcaa aatcatgtcc aacgccaaaa tctttttctt tcaaaacatt ttccattgtt
    40081 aaatgagcaa gttctttcat attgttttcc ttgctaagat ggccaagata gattcgtttg
    40141 gtccgattac cgatcacatc agtcatgaca agtgcaccgt cttcgttgga aagatgtcct
    40201 ctatctccta aaatacgctg ttttaaattc cacggataag gtcccatgcg taacatctca
    40261 agatcatgat tactttccat caaataacca tcggcatttt caatgacacc tcgcatataa
    40321 tcactgcaat atccggtgtc cgtcaacatg acgaatgacc gattttcttt atggaaacga
    40381 taaaattgcg gagccgctgc gtcatgggag acgccgaaac tctcaatatc gatatcgccg
    40441 aatgttctga ctttccccat ctcaaataga tgtttttgtt ccactggtac atttccaatc
    40501 agtggttcca tcgcttccca agtctgctga ttggcaaaaa catccaaatg atacttacga
    40561 gccaagacac ccacgccatg tatatgatct cgatgttcat gcgtcacaag tattccatct
    40621 aattggtccg gtgtccgatc aatttgagcc atcaaggaag tgattttttt accgctaagt
    40681 cctgcatcga ccaaaagccg ttttttttca ctttcgatat aaagtgaatt acccgaactt
    40741 ccacttgcta ggatactgat tttaaaagct ggtttttctt cagccatttg actcctactt
    40801 cccttcttac tttgtaaact tttttcatta tactcgcttt tttattgatc ttccactttt
    40861 ggtacagtat tgtttgtcac gatcgtattg ctgaaagcgt tgacgctctc aatttggacc
    40921 actttatcgc ttgtttcgat cgctacatgc caaacaggaa catacacatt tttttcccgg
    40981 actttcagaa tacaggaata agctagctga cgccataaaa tttttgatcc tcttggtatt
    41041 ttgttattga tgtacaatgt attgattgct tcctcttccg tatacagttc cgtcttttcg
    41101 cgcagctgct cgatatcaga taaatgcgtc tgcatgtatt tcgtgatgct ataatcatta
    41161 tcgttttttt ctaaagaaag aacgattttt gctgtatcat cattgaatgg gatgccttca
    41221 tactcttggc ttgcttggat ttctagtggt tcttcttcta aatgagaaaa gtttttcgtg
    41281 tacacataat cattaccgaa taaaatattg tctttgttag ataaaaaatc ttccaaggaa
    41341 ctactaattt tatctggatc gatcacgttc ttttcagcat ctgaactgga taaagatttc
    41401 gtcaaaacat caccgtctaa aaacacacca cttgccagca agccgctttt accagccttg
    41461 cgataattcg ctaatgaagt gctaagattg gtctgctccg cgctaagata atagccttgc
    41521 ttgttctcag acgaaatctt ccccgtgtaa gtgatatcgt cgctagccag acgttgttgg
    41581 attgattcag tctgattaga gcgaatgaca ttgttttctt ggtataccgc gtcatgataa
    41641 ctgctgaata aaaacacatt caaccctaaa aaagcgacga aaaatatcca ttcgattttt
    41701 ttgaaatcca attaattcgc ctccaatcca ggtaattttt ccagaaggtc gttagctgaa
    41761 taccagttat ctttatattt cacataccat tcaggtaaga gatcaacgac acgattcgtt
    41821 tctttgacat tttgccatgt atagccaacg atcactgaac caatcttgtt gctatctgct
    41881 cccttagctt ttaactgctg gatgatttca ttcgtacttg caagttctac ttcttcgtct
    41941 gaaggtatcg gcacttgaat cgtattcaaa ctggtttgga tattcacttg cacagaacca
    42001 ttcgtttcac cgccaacttc cacaccaatc tgtcctctgc tgtctgtccc aaaaaccggg
    42061 aagccttcca cgaatatccg ataatcgatc gtattgtcac tgcggtcaaa atatctgagg
    42121 ttccctaaag aactgcctaa tttgctaata taggggaaac tttcagaaaa tacactagca
    42181 ttttcttttt tctctggcag ctctccacga aaatcaacta cttgcgtttc cgaatggata
    42241 gacattgttt cattcccacc gtaaaaaatc aattcattgc tttcatcatt atttttcacg
    42301 ctatctggtt tctggaaaaa agcatttcta aaaagtgtat agggttgctg tgacaggata
    42361 taactatact tcttcagttt gatcgaatgt ttcgtagcat attgcgtgtc gatcgtcgat
    42421 ggttctgttg tcatttctgt ccattttgtg tcttttgctg ttaattcttt attgatatct
    42481 gtccaattca acgaaatatt tgcttcatat acgagatggt tcgaataatt caaaaatcgg
    42541 atttttttat ttccttgatc gaattgtatt ctagtaaaat acatattttc gctttctttt
    42601 gccaagttcg acacatctaa atctaaatca aatgcttcct tgtattctct tagcaaaaaa
    42661 ggcgcattat aggccaactc aaaaccagag ttcagatttt tcacctgttc aaactgtgat
    42721 ttgtctcctg aaactacttc agaaagcttt ccaaatcttg ctttatcgat gatcaattgc
    42781 aatcggtaca cgagattttc actgttggtt tcttggatca ttcccgaatg gtgccatgta
    42841 acatgaagcg gaagaaagac ttcggaagtt tttcgattgc tttgagactc gatcatttga
    42901 gtgtttgtct catctacgtt gattgaactt tttcctgctg ggctcagcca gattgcataa
    42961 gagaagtata tgcttaacgc aaccatcaaa atgagtccta tacgaacgac tttttcagag
    43021 attctcattc ccaccattcc tcctcataag gttcatatgg cagcgagata taaaaagtcg
    43081 acccttttcc ttccttgctc tctgcccaaa tagcgcctcg atgcgcctta atcacttctc
    43141 tagaaatagc taatccaagt cctgtcccac cttgtgctct tgcacgtgct ttatctactc
    43201 gatagaaccg ctcgaacact cggttcagat cttttttagg aatccccaat ccttgatcgg
    43261 tgatacttaa aatcacgtta ttatgcgttt ctaataagcg acaagtaatc gttcccccat
    43321 ctggtgaata cttgatcgcg ttattcatga tattatcgac tacttggatg attttatctg
    43381 tatctacttc gacccaaaga tctctttgcg taaattcacg acggatctta taattttttt
    43441 cctgatttcc caccatcata tcaaaccggt caagaacaaa attcactaac tcgttgaaat
    43501 tgacatattc taattgaagc tgggtattac ctgtatccat gcgagataga tttaagaggt
    43561 cattgatcat tcgaatcatt cggtctgttt catctagcgt taccttcaaa aaatttggtg
    43621 ctacttcctc gtctttccac gcaccttcgc tcaatgcttc gatataacta cgcatacttg
    43681 tcaacggtgt acgaagttca tgggaaacat tagagacgaa ttcacgacgt tcccgttctg
    43741 ttttttcttg ttctgtcaca tcatgcaaga cagctactaa tccgctgata aaacctgatt
    43801 cgcgacggat catagaaaag tctactcgca agatcatctg atcactttcc aatgtactgg
    43861 ttgaacggtc aagtaccatt tcgtttggat cttctaataa tttccgcaac gtatattctt
    43921 cttcgatatc taaaagtgtc aaaatcgatt gtccgattgc ttcctcgttt ttgatatcta
    43981 gcaaggaaac agccatatca ttgatcgtaa tgactttacc cctgcgatct gtcgcaatga
    44041 cgccgtctgt catatgggat agcacgctat ccaaacgatt gcgttcagct tccattgtat
    44101 cttgagcttc ttcaatacgt tcagctaact gattgaaggt atcagccagc tgtccaagtt
    44161 catcttgtcc atgcactttg actttccgac tgtagtctcc tctagcgatt cgaatggctt
    44221 gttctctcat ttcaccgatc ggctgtgtaa tcgaacgagc aactaaaaca gatacgatca
    44281 ttgaaataaa tgccgcaatt agtgaggcag taaagaaaat gactgctgtg ttattgattt
    44341 ccgaatattt ttgttcgata ttacttttga cgtaaagagc cccgataacg gcatcgccgg
    44401 tcggggacag gatcggttga acattgatat tcacccgttt atcgtcgtta tctaaagcaa
    44461 cataacgttt agtcgtgaaa tcattgatat caacataatc atttttcttt cccacagcgc
    44521 tttgttcatt gacatcggtt gttcctcgga tgatcccttt ttcgtcaacg acccgtattt
    44581 cgatgatatc gctagtcgca ctgttttcaa taaatctttt gatattcgca tttacctctt
    44641 catcatctgc attatctttt cgattcaatt ccgggctgat tgttgttgct aatgattcca
    44701 ccgtttgatt catatcttta ataaatgtat tgatcgtgga acgttctaaa ccacgtataa
    44761 agtaggcacc aataatttcg atcgagatca ataaaatcag aataaaggta atagcgatct
    44821 taaaattgac tgatcgaaaa aagcggactt ttcttttcat ccaattactc ctgttctggg
    44881 ttacgcaaat agtaaccgac accacgacgt gtcactaaat aatttggatg gcttggatta
    44941 tcttcgatct tttcacgcaa acgacgtaca gttacatcca ctgttcgtac atctccaaaa
    45001 taatcataac cccaaaccgt ttgtaataaa tgttctcttg tcatcacttg tccaaggtgc
    45061 ttcgccaagt aaaagagtag ttcgaattca cgatgtgtta actcgattgt ttctcctcgt
    45121 ttcgtcacca tgtaagcttc tggatggatc gtcaaatcac caatcgtcaa ttccgcaggt
    45181 gctgcctctt ctggttcctt cgcagccgtg gcaccgcgac gaagatttgc tttgacacgt
    45241 gcaactaatt ctcggttgga aaatggcttg gtcacgtaat catcggcgcc taactctaag
    45301 cctaggactt tatcaatttc agaatctttg gctgttacca taatgatcgg catatcataa
    45361 gttttgcgta cctcacgagc aacttctaag ccgtccattt taggtagcat caaatcaagt
    45421 aaaatcaaat caggttctac ctcagcaact ttttccaagg cttcttcccc atcatatgca
    45481 gtataaacgt catagccttc tttggctaaa ttgaatttga cgatatccga aatcggcttc
    45541 tcatcatcga caactaaaac ttttttcata aagagccacc tcttatatat tattgtcata
    45601 ccttctcatt ataccgtatt tctgcttttg tttcatcttt gcctataaaa caaaagagaa
    45661 aattaaaatg aaagtgcaaa atactttatt attttttgtt ctaacttcaa taaaaaagga
    45721 aaacactgat tttcacctca gtgtttcctt tttttgattt atccttctaa aacttgtaca
    45781 ccgacaccca ataatcccgg accagtgtgg actactaatg ccggagaaat ctggccaaag
    45841 tagatttggt ttgcttgtgg gaactctctt ttaagtctcg cttccatttc aattgcttct
    45901 tctttcgcat ccccatgggc aacagctaag ttgaagacct ttgcattacc gattttttct
    45961 ttgacgtaag caaccgtttt atccaaactt tttttacgtc ccctagcttt tcctactgtg
    46021 taataaatcc cttcttcatt acatgagatg attgggttaa gctttaaagc ggttcctaca
    46081 atagaagcaa ctaaaccgat ccgtccacct ttttgaagat attccaatgt cgcaacatta
    46141 aagaaaactt ttgttctttc gactaactca ttcagattac tgatcagttg atcccattct
    46201 actccttctt ccagccattt tgctgcttgg atcgcagaaa aacctgcacc aataccaata
    46261 tttttcgtat ctagtacata tgcttccaat tcttcttgct gttgtgcaat taaacgaacc
    46321 acattatatg tcccgctcaa tccactggaa attgttaccg caagtacctt ttcataaccg
    46381 tcttttttga tttgctcaaa gatttctgtg atcgactcac catccggcaa agatgtacca
    46441 gggatttcct ctggcaaacg ttgatagatc tcttcagggg taatgtcaat tttatctgta
    46501 tatacctgat ctttataaat aattttcaat gggatcatat acatctcgta tttttttatc
    46561 aattcttctg gaacatccgt acctgaatct actaaaattg cgattttatt gtttttcatt
    46621 tgttttctcc tcttttaaat attcggtttc tagaacaatc gttttttccg caagaagttt
    46681 cattgcaaac gataaagttg ccgctcttac agcaatatat tctacagggg ttgcctggtc
    46741 gaatttttgc ggatgagatt taccttgtgc taattggctg accatccaca cagctgcttc
    46801 ttgttcatcg caaaatagat tataggcttc acgaattcct actgtttttc cttggaacaa
    46861 tatcccttct ttgatttcag gaatagtcaa tacttgtttc aacgtagtga ttgcaagtaa
    46921 aaatgctacg tgttctttgt tatatctttt tttaactggt gctggtatca aacctagttt
    46981 aacgtaattg tttaccatag aagaggtaag caatggatgc ttttctcctt gtatcacagg
    47041 tgaaagataa cgatcaacta acgttctgac ttggtccata taaagttcta agtcagggag
    47101 ttcttcccag tgaggcagac ggaatgtttc tagttccttc ccccactcca ttaattgctg
    47161 acgcattttt tccatcataa ccgctccttt tttcatttca taaacatgat atcagactta
    47221 tgataaacat tcaatctagt tgctttaact aggtgatttg attagcggga ttccatgaaa
    47281 acaaaaaaca agaaggctaa gccttcttgt cttttatcgt taagaacagg agacccgccg
    47341 gggagacggc gggaaaggag taaaaaatga aaaaagtgtt agggtttgtt ctcttgcaag
    47401 aactttgttt gttggtatgc ttatataata gcgagcaaat gtgaataatc tatgaatgat
    47461 ttcctataag gaagtaaaga tttttctgat tttctttaaa tgtagtcttg cattttaaaa
    47521 ttcttagtgc tactattatt aagtactttt tgtacgggcc gttagctcag ttggtagagc
    47581 agctgactct taatcagcgg gtcgcgggtt cgagcccctc acggcccatt gggtgccaaa
    47641 cccacggaac aggtaaatgt tggtgcgaaa gcatttcgaa aacatttacg ctgttctttt
    47701 tttatttttc aaaataacta gggcttttta tttttaactc aaagtccact gtagcccttt
    47761 ggactttgag ttaaaaaaga agcataatta tgaacagtaa agtttctaat ataacaataa
    47821 ttcgagaagc tttgcaaaat catgctgcca atgaactctc taaacatata ggattaaatc
    47881 taagtactat aaaaggttaa aaagtgaaga atatttaata gaaaaactaa atttacttgc
    47941 tgctttcccc ttttccatta atacaacaat cagagagtgt ctttttttcg aaaaaattag
    48001 aataaataaa agacacgtag tttttttatc tacatgtctt ccatcgacta caagttatca
    48061 tttctcaaaa cgtatttagc ttttaattga ttaatcgttt cacgttgtct acacattgcc
    48121 taattaagat tttcttatta gaagcatcaa tcccgcaata atacctaaaa ctccaggaat
    48181 tacccacaat aatgttaaaa gaaaaaatgg aataatagat aaaatgagta aactaatacc
    48241 atagaccact gttttttgat tttttcttac taaaaaacct attatgaaaa tcattatcga
    48301 aaaaactagg gtaattcctc caaataaaac aacattcccg cttagattga ctaacatatc
    48361 tatatcctta ctggttagtt ctgtagtatt taaagtagtt gaatcaatag aagatagaaa
    48421 cataccaccc atcaatgcta aaagactaca taaaatccct gttattccac taattaaaat
    48481 caaaataaac tctgtttttc tcttattcat ttaattctct ccagattaag tgttttagaa
    48541 ttagaaattt tttgaccata gttaatagct tctaatgaac gaataacaac cactgacgtg
    48601 ttagaaaaat aatccccaat atgttgtttc ttgggcgtga aagcagccac caaataccca
    48661 atttgaaata caaaattttt taatataaac ctacatatac actcacgaat aaagatagtt
    48721 tgccaactta gctctttttc ctcaaagctc actactctta tcccaaaaat cattttccca
    48781 attgtttgtc ctgaattcag cttagtcaaa agtacaaaat aggttaaata aatagctaat
    48841 gacgctatac cataaaaagt aataatggat ttgtcagatt ccagtcccat gattttgtaa
    48901 aagagaccta tagtagcatg ggtgatgatg ctaatacaaa ttaaatcaat aagaaaagca
    48961 aaaaatcgta accaaaatcc cgcaaataca taattaggca aagtttcaat gccactcttc
    49021 ttattcattt tatagccctc catagtaata cattggtttt ggagtatcag aaaatccaag
    49081 tttttcaaaa actgaaatta cacgatctga tgtagttgcc tttagccctt gccattcagc
    49141 aatcttatta cctaaccaag aagaagcaaa gccagttgtg tcatttttat attctataac
    49201 cgtcgcatca gttaatttct tctctttttt tagggaatct aatgcatctt cagagtaacc
    49261 aatttgatcc accaacccat tttctactgc ttggttacca tcataaattc ttccatccgc
    49321 aagttctttc actgcattag tatccatatt tcttcctttt gctacaacat tgacaaaacg
    49381 atcataagca ctcatcacaa attcctgtat aactttattc tcttcctctg aaggtttgtg
    49441 ttgaggtgac atcatatctt ttaatgcacc acttttatac gtcgcatcag ttacgccaag
    49501 tttttctaat aagccagaat aatttaaacc agaaataatg acaccaattg atccagttgt
    49561 tgtttcttcc gtagcaaata ttttatctga accagcagaa atgtagtacc ctccacttgc
    49621 cgctgtattt ttaaatgcag tatagatagg aatatctagc tttttaattt ttgccatttc
    49681 tttagcaatc tcagcacttt catatattcc tcccccaggt gaatttactt ctaataacac
    49741 tcccttaacc gctttgtctt cttgaatttt ctttagctgt gttaaaaaat tctgatggtt
    49801 atattgttcc ctactaaaaa gattgctttc acctgtatct gcaataactc cattgacgga
    49861 tagtttgaca atctttttat ttgatgcgcc ttcttctaac gtttcttctg tgatttcgtt
    49921 agatccgtaa aaaatcttat ttattcctgt tagggttgta tttgatgctt cctctttatc
    49981 ttttcctgga attagcgcga cgataatgga tagtaacaat aatccgcacg ctactcccac
    50041 agcaatccat cttcttttat tcatataaac tcctcctata ttcttagtaa aaataattat
    50101 tttttattta gaaacacatc gacaatttgt tgccaatatg ttttttctcc tacaattaaa
    50161 gacaactctc cagttaatcc ataacgaaac ggaaacgatt gagttgtttt taacgttcct
    50221 ttcactgcat aaaaaacacc tttctcagtg gcagtagaat tttctgaaat ttcatctaaa
    50281 gttccgtcga tcgtaatcgg agccccacct ttttgatcca atttaaaatg aaccttcatc
    50341 ccttctttaa tataggtact ttcattcgca ggtaaaagtg ctgtaaattt catttttgat
    50401 ttttcgtgga tcggataaat ttctgcaagc actgttcctt tggctagttc tgcttgtccc
    50461 tttgtttctt catttaaatg aaccgttcca gatacaggag cttttagtat tccttttgaa
    50521 agttgatcgt ccaaactttt gataactgac tggttcttct cttgtttttc atttagctct
    50581 gtaactgttt gttttacagt tgttattgct tgttctagct cttgctttcc tttttccgtt
    50641 tgactaattc tgtcattctc agaagtagtt ggtttaacta atttagcttt ttctgactga
    50701 tactgttcta cttctttttt tagttgctca atttgttcat ttatgcttgc tgaaattgtc
    50761 agtttcactt gatttttttg atcatcagaa acattattta gttgttcttg ccaattctca
    50821 tagtttgcca tcatttcttt tgaaaaatct tttaatgatt gattattact ccaagctacc
    50881 tgtacttgtt gccattcttg taatttacta tctgaactct caatttgttt ttctagttgt
    50941 ctttttgtct tttcgtatac ttctaattgc ttttgatcat ttaattcact ttgcttcaaa
    51001 gcgtagcgga gtgattcttt ttctgacaac atgctcttta attgattact gtagccaaaa
    51061 ctgtcatccg ttgaaaacaa attttttcct ttacttatac tttcaataaa tagctgagcc
    51121 atctttattt gttgagtaat acgttggttt tcttgttcta tctgagcctt ttcattctct
    51181 attaaagaac aatccaatct aaccaacaca tcgtccttat gaacaaattg attttctttt
    51241 aggaagtttt ccattatttt agcttctaat ggcacttgta acttctctat tttttcgggg
    51301 ttcaattgag ccgttgtccg aataacaacc tcttttttag caaaggttaa aaacaatcca
    51361 ataatacata aaaaaagaat tacagggtat aacacccaac ggtagaagct atgatgttgt
    51421 ttagtataga tactagaatg ttcgaaccaa ttattattct gcataaattg tttccctctc
    51481 ctagatttcc cataattttt gatagatacc atcggcaaaa cgaagttcat cgtgcgttcc
    51541 tttttctacc aaatgccctt catggagaac taaaatttga tcacatgctt ttgctattgg
    51601 taaatgatgc gcaataaaaa taatcgtttt ttcttttaaa ttgaggagat aattcataat
    51661 tgcatgttct aacaaggtat ctaatccact agttgcttca tctaaaatta atatgtccgg
    51721 attttttaat aaagcccgtg ctaaggctaa tcgttgtcgt tgccctcctg aaagattatt
    51781 tccaccttct tccaacaatg tctcgtaacg taatggctgt tgattaataa attcatttag
    51841 ttggactatc tcacaaactt ctattatttg ttcgaaactt ggtgggctat ctaaaccgaa
    51901 tataagattc tcaatgattg ttccactaaa gaaaaatgac tcttgaggca aatatgtcac
    51961 tcgatcgcgt aatatctgaa aaggaatgtc taagtgatta atgtttccat aatggatcga
    52021 tccttctgat gcttcataaa aacgaacaaa taactttgca agagttgatt ttccagaacc
    52081 actaactcca actatagccg ttttactgtg cgaaggaatt gtacatgaaa tttcgttcaa
    52141 cgttggggcc ttcatgctat acgagaatga aacgtttttt acagtgatat cttgttgaaa
    52201 taattgttca gaaagcatat attcaaaaga ctggttcgat ttttctgaat caatcgcgaa
    52261 aatttcattt aaacgattat ttgcaacttg agccgtttgc attttcactt gtaaattaat
    52321 aatgttttgt aaaggatctg tcaaaaatac tagtaaagca ttgtatgtga taagttgacc
    52381 tagactaatt ctattatcca taacaaaata agaacctagc cataaaatta atgcactgct
    52441 aattaattga atcaaatgtt taattccttg ttgaatatta tcaagcgtaa ccgtacgaaa
    52501 tgattttttc attaatttaa caaattcatg gtctatacgg ttatatactt tctcttcacc
    52561 gctatatgct ttaattgttt ctattccttt aagactttct atgatactag aatttacagc
    52621 tgcaccggct gccatttctt ccctgttcga tttttcatag cttttcacga aagcgaatat
    52681 tgcaactaca taaaaaggca aagagcatag agtgattaaa aacaacgtac tattctgaat
    52741 agctaacgta accccaacaa gcaacaccat accaatatct aaaaatattg ataacgtagc
    52801 acttgctaaa gcatcaataa tcttactggc atcaagaaaa cgagaaataa tttcccctga
    52861 ttttctcgta gcaaaaaaat tcattggtaa agttaacaca tgtttgaaat actgaagcat
    52921 aattgccata ctcattcgct gtcctaaaat aataagaaga taactacgac tatattcaaa
    52981 caatacccga aataaataga cagcgatcaa gccaatggaa attatattta aagttgaacg
    53041 tgcttgatta gggatgaaaa aatcaagaat accttgaaaa taatatgagc ttcctatgcc
    53101 aaaaaacgta atgaaaatcg atgctaaaac gatgtggaat actaatcctt tttgtttcca
    53161 aattataggc aaaaaagaac ttaatccagc tactttttct ttaataggaa tataagtttc
    53221 tctaggcata ggtaataaaa gaatacctgt ccattctttt tgaaacgctt caatcgtttt
    53281 tctatttttt ccattagcgg gatctgcaat ccatagtacg tttcccttcg ttttataaat
    53341 tacaacgtag tgcatatagc tgttatcaac caatatatgt gcaattaatg gtaagataag
    53401 atctctttct ttccagatct cactatctgc ttgaatagcc atacaatcaa aagacaattt
    53461 ttcaaatgtc tttttcaatc caaatgctga agtaccctct aaatcagtcc cagataggtc
    53521 ccgtaattta tgaatcggga tatcagtttt aaaatatttt aaaatcatcg aaaggcaagc
    53581 aacaccacaa tctttttcat cttgttgctg cacaaaagga aatgatttta tcacttatct
    53641 aacacaactc cttctaaaaa atttttatag aaaaattctc aatatagctt taatacagtg
    53701 aaaatattgt ctcataattc cacaaaaact ttgaaaacag ttcttgaaca ctaacttttt
    53761 cattcctgaa tcaaatttat tttggaaatt ttactattca agaataaact tgataagttt
    53821 caggaagatt tttactgtta atcaaatacg tatgttacag tagcaccgta aaaaatatta
    53881 tttaaagaag cgtgtttaaa catgcaaaat gtaaaggaac taagtattgt ggaaatgcac
    53941 aaactgttgg tggaggcaat aatgcttggg gaaaattggg gcaagttgta ggtggactta
    54001 ccacaggggc agtaggtggt gcaggtttag gaacagcaat ttgcggacca gcttgcggtg
    54061 ttgttggagg gttatatgga gctgtcgctg gaggtgctgc agctggctgg gatgctcgaa
    54121 aaaaataaaa aaggagaata agtagtatgc atattaaaaa cacaaaaaca acatttatat
    54181 tatcttcaga agagttgaaa aatattcaag gtgggagtgc cgtgggagtt ttaggcacta
    54241 ctttttctgg ggcaacagca ggagtaaaac tttgttcagc cggaggacct tatgcaatag
    54301 ctgcttgtgg tgtaggtgga gctttattag gtgctggatt cagcatgtgg accggtgcgt
    54361 aaacaattaa aaaatatcaa acctcaagat tggtttgtgt cgattacgaa cataggtatg
    54421 ttctttatct tttcaaaagt ttggaatgga cagaactttt tactaaagct tcttgtgcta
    54481 gtttttataa ttatattaaa tttttatctt ttattaaagt cagataaaaa ttctttggaa
    54541 atgtaatggt agagatattt ttacaaaagg acttgcagcg ctgtagttgg tgctgcagga
    54601 ggacctattt cggccgttac aggaggtatt ggtaatgtac ttgtcggatg tgcattaaag
    54661 cctgttcctg caagttaaat ttataaaaac aggaaagtaa ttatctcaaa tcctatcaag
    54721 tacaatcata ggattgcact tgacaggaca aattttttaa ctaaaaaatg aaaaacactt
    54781 tctaaaaatt agtagaattt gccaaatctt tcgcatcttc caccatacat ttaattttaa
    54841 ttccccttta tggtacttaa taaaaacaag aaaagaacta ttcatttggc ccttttttgg
    54901 ttccagacca tagaaatatt cctccagtaa ctaatattgc tgttatgcac gtaactaata
    54961 acagctataa aaattggtac aactttaata ggattcagaa aaatttgcac tagtgaagga
    55021 atctatttta ttagaaaaat gcaatctccc taaagaaaaa accactaaac agtgctttct
    55081 ttcaacttct ccttaatttt ttgtgctttc cctaaagtaa agtttctggc caactctgaa
    55141 tcaaaatata gtgaacgttt tttaaaatct atttctttaa cgttaaagat atttactaaa
    55201 cacgctcgac taattcttgt taacatagga tatttctttt caagctcact aattcttcca
    55261 taaaattcgt actgcccatc tttagtacac aatgataatc tatgtggtaa ttttgaagct
    55321 tctacaaaat aaatgtcatt tatatcaaaa gtaaatgttt gagaacctat ggaaaaaaca
    55381 aatgcttgat tcgtaacctg ttttgttgca tctatccttt cttgcgctaa taacaataac
    55441 tcaacaatct cgtttcgata ctcatctagt gtttgatctt ttgttacaaa tcctaacgtt
    55501 tcaactcttc tttttattgt tacgggtaac atctcatcat gagtagttgt aaaaatgatt
    55561 tttgcttgaa catcatattt tctgattact tctgctaatt ctataccgtt aacttcatga
    55621 tttaaatcaa tatctaagaa atatattcct tgctttggtc ggaattgttt gaggtatttt
    55681 ttaacttcta acggactttg tgttttcaat actatcttaa atactttatc gtgaaaaaga
    55741 atgaaattat caataattcg ttctaactga tttaattgta taatttgatc ttcacataat
    55801 attattggat aactcataaa tcctccttcc ctcaaaacaa caaaataact tgagtagtaa
    55861 attgatacgg atcgttttga tagttcacaa acatattggg attcttttta ttgatttcct
    55921 ctatactaga taaacctaac ccttgatgtc ccttttttgt tgttattcct tttttcatta
    55981 gagtatctat agagagtagt atctctctac aactattcga tatcgaaatt tctatctgtt
    56041 tatcatctcg gtaaatagct agagacagtt ctctatcttg actctctata gatgcttcaa
    56101 ttgcattatc gagaataatt ccaagcaccc taatacagtc aaatattgga acaggaactt
    56161 caataatagt acttggacat tcgaatttac aacaaatctt attgttattt gcttggtata
    56221 gcttcgctat caacaaactt ttcaaatatt cattttttat attttttaaa taacgatatt
    56281 caaacttatt ggttagataa gaattagaat agatctctaa ttcttttatt tctttttcca
    56341 atatcgaatc atggttttga ttagcgattt cttttaaact cagcaataaa tttttatagt
    56401 catgtctaaa tctggcaaat ttttcttgat ctttttccag tgaatcagta tattttttta
    56461 gataaaccaa ttcttttctt ttcaattgct gttcatattg ttctcgttgc ctaatagtta
    56521 ttctaataaa taaaaataga acaacaaaca tttgaatgat taaaaatatt aagacaccaa
    56581 acacaaaatg atcaaatact tgatattgat gagcaacata agaaacaaat aaaccaattg
    56641 taagtaaata aatcatgaat atagcagtaa gtttagaact ggactgttca acaatcctat
    56701 ttatagctaa ttttctatat aaccaaataa acatcagcaa aaacaaagaa tcaagcatta
    56761 tctggaatac tacataacca taccctttta tcccttcaaa tgaaaacata tacgtcataa
    56821 ccatggaagt aataatagat ctgaaatcat caataagcat acataatagt aatttattta
    56881 aaagcactat attagttttc ttcttttcct ttaatataaa ataacttccg attataggta
    56941 tagctgcgga aacatcagta aataattcaa caaaaaaagt gagtggtatc aataaacata
    57001 caaaaagata ttttcttttg ttaagatcag tattagtcat ataaaataag agaaaaaaga
    57061 aaaagtttcc cacgaacatt cttaatagat aagcttttgt atcgatagta atcaagagat
    57121 cccctcctga ttttaggata tcataaaaaa aattttagtt acacttttta aataatgaaa
    57181 aaacacattc cactaaattt gatgccggtt ttccttgagg ttttgttcca gcacctcctt
    57241 taattttttt aagctcttga tctgaacatt gtttagcatt tagtctattt ttttcttcca
    57301 ttacgattcc ccctacaaac tataaatact tttatgtcac
//
LOCUS       DAZKOM010000008        50072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000008 DAZKOM010000000
VERSION     DAZKOM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.008"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..262)
                     /locus_tag="ACG8NF_000503"
     CDS             complement(<1..262)
                     /locus_tag="ACG8NF_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0471015.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTA"
     gene            complement(264..461)
                     /locus_tag="ACG8NF_000504"
     CDS             complement(264..461)
                     /locus_tag="ACG8NF_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0471016.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(855..2582)
                     /locus_tag="ACG8NF_000505"
     CDS             complement(855..2582)
                     /locus_tag="ACG8NF_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0471017.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2575..3768)
                     /locus_tag="ACG8NF_000506"
     CDS             complement(2575..3768)
                     /locus_tag="ACG8NF_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0471018.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3955..4599)
                     /locus_tag="ACG8NF_000507"
     CDS             complement(3955..4599)
                     /locus_tag="ACG8NF_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0471019.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4693..4827)
                     /locus_tag="ACG8NF_000508"
     CDS             complement(4693..4827)
                     /locus_tag="ACG8NF_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0471020.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="ACG8NF_000509"
     CDS             complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="ACG8NF_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0471021.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6958..7431)
                     /locus_tag="ACG8NF_000510"
     CDS             complement(6958..7431)
                     /locus_tag="ACG8NF_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0471022.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7832..8056
                     /gene="nrdH"
                     /locus_tag="ACG8NF_000511"
     CDS             7832..8056
                     /gene="nrdH"
                     /locus_tag="ACG8NF_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0471023.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8215..10374
                     /gene="nrdE"
                     /locus_tag="ACG8NF_000512"
     CDS             8215..10374
                     /gene="nrdE"
                     /locus_tag="ACG8NF_000512"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0471024.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10424..11389
                     /gene="nrdF"
                     /locus_tag="ACG8NF_000513"
     CDS             10424..11389
                     /gene="nrdF"
                     /locus_tag="ACG8NF_000513"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0471025.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11478..12617)
                     /locus_tag="ACG8NF_000514"
     CDS             complement(11478..12617)
                     /locus_tag="ACG8NF_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0471026.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12926..13639)
                     /locus_tag="ACG8NF_000515"
     CDS             complement(12926..13639)
                     /locus_tag="ACG8NF_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0471027.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="ACG8NF_000516"
     CDS             complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="ACG8NF_000516"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0471028.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14828..16360
                     /locus_tag="ACG8NF_000517"
     CDS             14828..16360
                     /locus_tag="ACG8NF_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0471029.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16551..17753)
                     /locus_tag="ACG8NF_000518"
     CDS             complement(16551..17753)
                     /locus_tag="ACG8NF_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0471030.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17835..18524)
                     /locus_tag="ACG8NF_000519"
     CDS             complement(17835..18524)
                     /locus_tag="ACG8NF_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0471031.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18630..18998)
                     /gene="rplL"
                     /locus_tag="ACG8NF_000520"
     CDS             complement(18630..18998)
                     /gene="rplL"
                     /locus_tag="ACG8NF_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0471032.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19051..19554)
                     /gene="rplJ"
                     /locus_tag="ACG8NF_000521"
     CDS             complement(19051..19554)
                     /gene="rplJ"
                     /locus_tag="ACG8NF_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0471033.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19758..19885)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19964..20653)
                     /gene="rplA"
                     /locus_tag="ACG8NF_000522"
     CDS             complement(19964..20653)
                     /gene="rplA"
                     /locus_tag="ACG8NF_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0471034.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20757..21179)
                     /gene="rplK"
                     /locus_tag="ACG8NF_000523"
     CDS             complement(20757..21179)
                     /gene="rplK"
                     /locus_tag="ACG8NF_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0471035.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21522..22190
                     /gene="sdaAB"
                     /locus_tag="ACG8NF_000524"
     CDS             21522..22190
                     /gene="sdaAB"
                     /locus_tag="ACG8NF_000524"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0471036.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22203..23075
                     /gene="sdaAA"
                     /locus_tag="ACG8NF_000525"
     CDS             22203..23075
                     /gene="sdaAA"
                     /locus_tag="ACG8NF_000525"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0471037.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23166..24266)
                     /locus_tag="ACG8NF_000526"
                     /pseudo
     CDS             complement(23166..24266)
                     /locus_tag="ACG8NF_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24358..25245)
                     /locus_tag="ACG8NF_000527"
     CDS             complement(24358..25245)
                     /locus_tag="ACG8NF_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0471038.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25322..25867)
                     /gene="nusG"
                     /locus_tag="ACG8NF_000528"
     CDS             complement(25322..25867)
                     /gene="nusG"
                     /locus_tag="ACG8NF_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0471039.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25968..26138)
                     /gene="secE"
                     /locus_tag="ACG8NF_000529"
     CDS             complement(25968..26138)
                     /gene="secE"
                     /locus_tag="ACG8NF_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0471040.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26162..26314)
                     /gene="rpmG"
                     /locus_tag="ACG8NF_000530"
     CDS             complement(26162..26314)
                     /gene="rpmG"
                     /locus_tag="ACG8NF_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0471041.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26476..27120
                     /gene="cbpA"
                     /locus_tag="ACG8NF_000531"
     CDS             26476..27120
                     /gene="cbpA"
                     /locus_tag="ACG8NF_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0471042.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27176..27679)
                     /locus_tag="ACG8NF_000532"
     CDS             complement(27176..27679)
                     /locus_tag="ACG8NF_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0471043.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27692..27738)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28276..29241
                     /locus_tag="ACG8NF_000533"
     CDS             28276..29241
                     /locus_tag="ACG8NF_000533"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0471044.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29340..30512)
                     /locus_tag="ACG8NF_000534"
     CDS             complement(29340..30512)
                     /locus_tag="ACG8NF_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471045.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30499..31644)
                     /locus_tag="ACG8NF_000535"
     CDS             complement(30499..31644)
                     /locus_tag="ACG8NF_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471046.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31658..32582)
                     /locus_tag="ACG8NF_000536"
                     /pseudo
     CDS             complement(31658..32582)
                     /locus_tag="ACG8NF_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32699..33778
                     /locus_tag="ACG8NF_000537"
     CDS             32699..33778
                     /locus_tag="ACG8NF_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0471047.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33775..34403
                     /locus_tag="ACG8NF_000538"
                     /pseudo
     CDS             33775..34403
                     /locus_tag="ACG8NF_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34513..34740)
                     /locus_tag="ACG8NF_000539"
     CDS             complement(34513..34740)
                     /locus_tag="ACG8NF_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0471048.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34843..37431)
                     /locus_tag="ACG8NF_000540"
     CDS             complement(34843..37431)
                     /locus_tag="ACG8NF_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0471049.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37451..38110)
                     /locus_tag="ACG8NF_000541"
     CDS             complement(37451..38110)
                     /locus_tag="ACG8NF_000541"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0471050.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38159..38662)
                     /gene="trmL"
                     /locus_tag="ACG8NF_000542"
     CDS             complement(38159..38662)
                     /gene="trmL"
                     /locus_tag="ACG8NF_000542"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0471051.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38883..39749)
                     /locus_tag="ACG8NF_000543"
     CDS             complement(38883..39749)
                     /locus_tag="ACG8NF_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0471052.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39858..40499
                     /locus_tag="ACG8NF_000544"
     CDS             39858..40499
                     /locus_tag="ACG8NF_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0471053.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40561..41922)
                     /gene="mgtE"
                     /locus_tag="ACG8NF_000545"
     CDS             complement(40561..41922)
                     /gene="mgtE"
                     /locus_tag="ACG8NF_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0471054.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41940..42839)
                     /locus_tag="ACG8NF_000546"
     CDS             complement(41940..42839)
                     /locus_tag="ACG8NF_000546"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0471055.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42841..43638)
                     /locus_tag="ACG8NF_000547"
     CDS             complement(42841..43638)
                     /locus_tag="ACG8NF_000547"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0471056.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43616..44320)
                     /locus_tag="ACG8NF_000548"
     CDS             complement(43616..44320)
                     /locus_tag="ACG8NF_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0471057.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44451..45027
                     /locus_tag="ACG8NF_000549"
                     /pseudo
     CDS             44451..45027
                     /locus_tag="ACG8NF_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45145..45822
                     /locus_tag="ACG8NF_000550"
     CDS             45145..45822
                     /locus_tag="ACG8NF_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0471058.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45901..47709)
                     /gene="pepF"
                     /locus_tag="ACG8NF_000551"
     CDS             complement(45901..47709)
                     /gene="pepF"
                     /locus_tag="ACG8NF_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0471059.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47885..48883)
                     /locus_tag="ACG8NF_000552"
     CDS             complement(47885..48883)
                     /locus_tag="ACG8NF_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0471060.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49109..49762)
                     /locus_tag="ACG8NF_000553"
     CDS             complement(49109..49762)
                     /locus_tag="ACG8NF_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0471061.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14992 a        10503 c         8779 g        15798 t
ORIGIN      
        1 cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc
       61 ttattttatt agtataagca gtaaaatata gataattcac caatctattt attaaaggac
      121 cgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
      181 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
      241 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
      301 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
      361 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
      421 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
      481 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
      541 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
      601 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
      661 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
      721 gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
      781 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
      841 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
      901 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      961 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
     1021 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
     1081 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
     1141 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
     1201 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
     1261 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
     1321 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
     1381 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
     1441 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
     1501 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
     1561 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1621 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     1681 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     1741 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     1801 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1861 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1921 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1981 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     2041 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     2101 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     2161 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     2221 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     2281 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     2341 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     2401 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     2461 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     2521 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     2581 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     2641 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     2701 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     2761 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     2821 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2881 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2941 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     3001 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     3061 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     3121 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     3181 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     3241 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     3301 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     3361 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     3421 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     3481 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     3541 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3601 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     3661 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     3721 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     3781 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3841 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3901 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3961 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     4021 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     4081 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     4141 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     4201 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     4261 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     4321 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     4381 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     4441 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     4501 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     4561 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4621 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     4681 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     4741 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     4801 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4861 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4921 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4981 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     5041 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     5101 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     5161 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     5221 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     5281 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     5341 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     5401 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     5461 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     5521 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     5581 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     5641 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     5701 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     5761 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     5821 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5881 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5941 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     6001 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     6061 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     6121 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     6181 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     6241 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     6301 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     6361 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     6421 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     6481 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     6541 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6601 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     6661 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     6721 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     6781 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6841 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6901 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6961 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     7021 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     7081 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     7141 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     7201 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     7261 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     7321 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     7381 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     7441 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     7501 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     7561 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7621 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     7681 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     7741 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     7801 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7861 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7921 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7981 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     8041 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     8101 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     8161 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     8221 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     8281 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     8341 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
     8401 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     8461 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     8521 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     8581 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     8641 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     8701 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     8761 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     8821 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8881 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8941 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     9001 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     9061 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     9121 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     9181 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     9241 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     9301 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     9361 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     9421 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     9481 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     9541 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9601 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     9661 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     9721 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     9781 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9841 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9901 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9961 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
    10021 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
    10081 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
    10141 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
    10201 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    10261 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    10321 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    10381 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    10441 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    10501 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    10561 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10621 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    10681 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    10741 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    10801 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10861 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    10921 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10981 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    11041 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    11101 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    11161 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    11221 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    11281 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    11341 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    11401 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    11461 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    11521 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    11581 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    11641 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    11701 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    11761 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    11821 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11881 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11941 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    12001 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    12061 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    12121 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    12181 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    12241 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    12301 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    12361 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    12421 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    12481 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    12541 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12601 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    12661 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    12721 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    12781 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12841 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    12901 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    12961 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    13021 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    13081 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    13141 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    13201 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    13261 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    13321 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    13381 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    13441 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    13501 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    13561 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    13621 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    13681 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    13741 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    13801 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    13861 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    13921 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    13981 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    14041 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    14101 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    14161 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    14221 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    14281 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    14341 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    14401 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    14461 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    14521 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    14581 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    14641 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    14701 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    14761 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    14821 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    14881 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    14941 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    15001 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    15061 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    15121 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    15181 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    15241 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    15301 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    15361 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    15421 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    15481 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    15541 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    15601 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    15661 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    15721 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    15781 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    15841 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    15901 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    15961 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    16021 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    16081 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    16141 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    16201 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    16261 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    16321 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    16381 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    16441 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    16501 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    16561 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    16621 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    16681 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    16741 ttgcttgctg cttcacctag cccctgtact cgtgtttgat tggcgacttg accaaattga
    16801 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    16861 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    16921 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    16981 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    17041 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    17101 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    17161 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    17221 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    17281 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    17341 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    17401 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    17461 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    17521 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    17581 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    17641 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    17701 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    17761 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    17821 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    17881 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    17941 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    18001 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    18061 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    18121 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    18181 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    18241 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    18301 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    18361 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    18421 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    18481 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    18541 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    18601 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    18661 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    18721 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    18781 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    18841 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    18901 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    18961 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    19021 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    19081 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    19141 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    19201 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    19261 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    19321 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    19381 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    19441 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    19501 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    19561 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    19621 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    19681 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    19741 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    19801 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    19861 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    19921 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    19981 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    20041 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    20101 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    20161 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    20221 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    20281 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    20341 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    20401 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    20461 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    20521 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    20581 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    20641 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    20701 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    20761 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    20821 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    20881 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    20941 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    21001 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    21061 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    21121 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    21181 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    21241 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    21301 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    21361 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    21421 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    21481 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    21541 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    21601 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    21661 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    21721 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    21781 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    21841 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    21901 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    21961 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    22021 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    22081 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    22141 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    22201 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    22261 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    22321 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    22381 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    22441 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    22501 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    22561 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    22621 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    22681 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    22741 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    22801 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    22861 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    22921 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    22981 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    23041 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    23101 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    23161 ttttattaat ccatcaattt cgtatatttt acttcatcga tcgtttttgt tcctgcaagt
    23221 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    23281 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    23341 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    23401 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    23461 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    23521 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    23581 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    23641 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    23701 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    23761 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    23821 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    23881 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    23941 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    24001 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    24061 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    24121 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    24181 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    24241 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    24301 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    24361 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    24421 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    24481 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    24541 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    24601 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    24661 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    24721 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    24781 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    24841 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    24901 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    24961 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    25021 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    25081 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    25141 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    25201 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    25261 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    25321 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    25381 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    25441 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    25501 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    25561 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    25621 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    25681 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    25741 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    25801 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    25861 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    25921 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    25981 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    26041 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    26101 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    26161 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    26221 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    26281 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    26341 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    26401 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    26461 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    26521 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    26581 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    26641 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    26701 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    26761 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    26821 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    26881 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    26941 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    27001 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    27061 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    27121 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    27181 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    27241 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    27301 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    27361 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    27421 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    27481 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    27541 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    27601 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    27661 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    27721 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    27781 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    27841 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    27901 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    27961 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    28021 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt
    28081 tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga
    28141 attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa
    28201 agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa
    28261 tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc
    28321 ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac
    28381 tatacacttc attatagtga atacaaagga cacgatgctg agattgctga aactttggca
    28441 aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt
    28501 ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg
    28561 gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg
    28621 gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca
    28681 gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt
    28741 aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca
    28801 aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt
    28861 gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt
    28921 ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg
    28981 attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc
    29041 aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg
    29101 aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat
    29161 ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact
    29221 aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt
    29281 ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt
    29341 taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg
    29401 atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct
    29461 gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa
    29521 tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca
    29581 aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt
    29641 tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac
    29701 aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac
    29761 agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag
    29821 gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc
    29881 tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac
    29941 ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat
    30001 cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct
    30061 gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact
    30121 tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc
    30181 atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca
    30241 cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact
    30301 gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta
    30361 atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa
    30421 aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt atttgcttta
    30481 agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt
    30541 tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc
    30601 caaatcagtg tagaaataaa gtttatacaa actttcgctg atcaagttga cgagatttaa
    30661 ttgaccaaca atcggaatat gaagatcaat ccaatacttc acttgttccg accccatcat
    30721 accagcaaaa aagctcatcg caatcgtcac tgaaatcaag atgctatcct tttgcaccag
    30781 attcattttt ggtatcaaat taccaaccaa tgtacctaac aaaatcgcac acaacgaccc
    30841 cagagcggta acaatcatca accattgcca acgttcacca aagtctactt gatagataaa
    30901 acgaaagaca gctaacataa tataaatctc tgtcaaaaca atcgtaaagc ttgctgccaa
    30961 attagcgatt gaaactaacg attgctttct aggaatcaag gacaagcgaa taccatttga
    31021 cgattgattt gcttgctgat cctgtacatt gcggaccccc cacatcgtcc cgtagagaat
    31081 cgccattcca atcagagtga agaaataaaa acttttgaaa ctcatattac gacctgcatg
    31141 aacttcttga aaagatactt cttgttcaaa aacagtcatg atgggcgcga gatcaacgtt
    31201 ctccacttct gccactttac tcgaaattat accaatacgc tgtaaaaatt gatccatgac
    31261 gtttttcatc aacgtctgac tcattcctgc ttgacccaca tggagcgaaa cagatgaacc
    31321 atcaatgaga taaaaaccag ccaaatcatc tgcatttagc ttttcattcg cttctacttc
    31381 cgatagttcc tgtacttcaa ataatggttt atcattgact tcgacttgtt ctaagactgt
    31441 ggtaaaattt tccgaaagcg ctgtttgatc cgctttgatt acaattccag tattaatcgt
    31501 ttcgagattg ccaccttgat caatctcgcc aaaggccgcc ataaacatca atcctaaaag
    31561 aattggaaag gccaatgtcc aaaacaacat ggatttattt cgcagcagta tttttgtgcg
    31621 atatttataa aaatgccaaa acattatttc ccctcctcta gtctctcaaa tccttgccag
    31681 taatctctaa aaagacatca ttcaaggacg cttctttggt tgctattttt ccgtaactga
    31741 tcttttcttc ttgtaagatt gtaagaatcg gcaacaatac tgtatttagt tcagtagttg
    31801 ttacttggag ctgacctttt ttataagcga ttccatcaga agctattgtt cgctgacaac
    31861 acgttttctg ggagtaatgg gatgtcgatc gtcaaacgat ccgccgtccg taccatatct
    31921 tttagagatt ctttggttcc tttagcaatc acctgtcctt gatccatgat cacgatctgg
    31981 tcgcaaagaa tttctacttc ttccatgtaa tgtgttgtat agacaatggt tgctccttgt
    32041 cgattcagct ccttgatact ttctaaaatt ttattccgac tttgggggtc tacagctact
    32101 gtcggctcat ctaaaaaaat taaccgcggc ttgtgggcaa tcccacatgc gatgttcaac
    32161 cgacgtttca agccaccact aagttctcct ggacgaaacg aaacatattt ttctaatccc
    32221 actaattgaa tcacctcttc aatcaaggct ttccttttct ctttttcgga aacatataaa
    32281 ccgcaaaaat aatcgatatt ttcttgcaca cttagttcgt caaagaccgc aacgtcttga
    32341 ggaacgaccc caatcattgc cttgacagca taattttcag gagtcattgt tgtcccgaac
    32401 aatttaatgg ttcctttgtc ataatttaac aaagaaagca tacagttgat ggcagtcgat
    32461 ttcccactgc cgtttggtcc taacagacct aagacttctc cttcttgtac ttgcagatca
    32521 aaatgattca atgctacaaa atctccatag cgtttgacta atttatcaat ttcgataatc
    32581 atcatgaaaa actccttcca aatttcttgt tgatctgact ataataaagt gcaagaaaag
    32641 aaactagtga cgattggcta aaccacacat gacatttgtc acaatgaagg agtctgctat
    32701 gcataaatct cggctactca acgaaactag ctttctactg gttatctcat tgcttttgct
    32761 ggtaagtcaa ggattcgttg ctatgcaagt tatctattta ttgatcgccg ttttattcac
    32821 tggtgtcagt tatttactgc ctacaaagcc aaattggatt ttccaagtta ttctatttct
    32881 tttatgtgcc tttttacctg atcttgtcct gtttctgcca gcgacgatgc gagtgatcgc
    32941 ccctgaagaa agccaccttg attacttgtt tttgttaggt tttttgttga tttcgacggt
    33001 tggtaatcat ccccccatca aggtcgcctt tgtcgtaatt ttgctgtttt tttcctttta
    33061 cttacgaaaa aaagatcaac gaacgaccga tcttgagaaa aagtacattg tctccaaaga
    33121 tactcattgg gaaaaacaac aagagttatc ggaacaaaat aaagcattgg ccctgtcaca
    33181 agaaacacaa ttagccttgc aaattgtgga agaacggaac cgaattgccc gagacatcca
    33241 tgacaatgtc ggtcatctgc tgtcaagtgc gattttacaa attggtgcgc ttgaaatgat
    33301 caatcaagaa ccaaaactaa aagaaccatt agaaaattta agtgccaccg ttcacactgg
    33361 gatggatcgg attcgtcaaa gtgtccatga cctccatgag acatcgatct cttttcaaca
    33421 ctccttagag tttctgatcg ctgattttcc ttcttctgtt acgattgaag gaaacttgtt
    33481 tgaaggattg aatgaaacgc agcaaaactg cttcttaatg gtcatcaaag aagccttaac
    33541 caacagtatc aaacatagtc atgccaaaac cgttgtttta cattttcgaa cgttgccggc
    33601 cttttatcgc ttgcaaatca cgaatgatgg caccagccca caaaaaaatt ctgctcccgg
    33661 tattggtttg aaaacgatgc gtcaacgtgt tcaagaacaa aagggacaag tccatatttt
    33721 ccaagaaaac cagcaatttc agttaacgat cattttacca agggagggac accaatgata
    33781 acagtagtca ttattgagat gatccatttg tgacgaaatc gttacaaacc attttagaaa
    33841 gcacgaaaga gatccgtgtg ttaggcattg gtcattgtgc caaggatgcg ctagtcttat
    33901 acgaaaccca tcgtcccgat gttttgctga cggacatccg catgcctaaa caaacgggca
    33961 tcgatgctgc caaagaaatt ttggctaaat ttcccaaagc catccttttg cttttgacga
    34021 cattcaaaga tgaagaatac atccgcgaag cattttccat cggtgtcaaa gggtatttaa
    34081 tcaaacagaa cttgcaagcg attattcctt ctgtcaaatc cgcttataat ggacaggccg
    34141 tatttggtaa tgaaatcatc gagactttca ctcaaatgat gaagaataaa ccgacgattg
    34201 atagtcatgc ttatgcttct tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg
    34261 ccgggaaaaa caataaagaa attgcagatg ccttgtactt gagtgacggc actgttcgca
    34321 attacatcag ccaattacta gaaaagctag atttacggga tcgcacgcaa ttggctatct
    34381 atttttatca gcatttgcaa taagtcttat ctaacacaaa aaaacctgaa tgatgctcct
    34441 tcgcatcatt caggtttttt tcacatcttc tattcaattg tcccgcaaac catttcctca
    34501 cgattgaaag aatcaaggat tcgcagggct gccaggattc gttggattgt tcggcgtgtt
    34561 aggcggaaaa ataggcgcct tgtctaacgt tttttctagt tttgattctt cacgatcgtc
    34621 tcgttctaca tcaggacttt cattcaattt gaattctttt tccatctcat ctaacttttt
    34681 aaagtcattg ccagattctt ttgctaattt cgctaattta tcttcggttt ctttagccat
    34741 aaagtaacgc cccccttttg atttcagctt agcatatcac actgaaaaaa caaaagaaaa
    34801 cgctaaccaa cgatcatttt tagctatttc tcatctttaa aatcaagcat tttttgccgg
    34861 tataaaatcg tagggagaac tgttttccat accaatcatc ggatctacta actggttatc
    34921 gatcacttct gctgtaagaa cgaaatcttt agaaacagca ggttctacct cttctttctt
    34981 ttcttctcca acaaatgaag aaaacagatc tggttccact gtttccatta cttggttttt
    35041 cgtttcattc gtcccactgt catcttctac aggtagcttt tcttttacaa cccgtggctc
    35101 ttttgtatag ttcccatctt caaatggatc atttccgacc aaagaatctt cataagctgc
    35161 tagtttggtc cggagagtga tattcatttt atcattatcc atgatccttg atttcaacgt
    35221 agtcatccgg cttgctgatt cattcgctac acaagtgaca taggcttgct cttcccctaa
    35281 caaaatcaac agttccttac tgcgagtcac tgccgtgtag agcaaattac gctgaagcat
    35341 ccgctgatat tgatggacca tgggcaaaat aaccatcttg aattcactgc cttgtgattt
    35401 atggattgag caacaataag aaagagtgat tttattccat tcgtttcttt tataagtgac
    35461 ctcatttgca tcaaactgga taactaattc atccacttta tcttcagaat cttttgccaa
    35521 gatgataccc gtgatttctc ccatatctcc attgaataca ttcaattcgg gagtattgac
    35581 taattggagg actttgtctc ctatccgata aacggtgtcg ttccaatgta cttccttttt
    35641 tccaggtttc cctggattga atatctcctg catcatttta tttaacgcat cgatccctgc
    35701 aggtccccga tacattggtg ctaatacttg gatatcttgt gcacggaatc ccttattttt
    35761 cgctttttcc acaattttag agatcaaggg ttcaatttga taagcatcac ttcggaaata
    35821 ggaacgatct ttttgattct ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg
    35881 ggcaagagga ataatactag aaccatcacc ttgccgataa atctgattga gttccatttt
    35941 gggaatttgt tcgatttcca aaagatcatg gagtacttga ccaggaccga cagagggaag
    36001 ctgatctttg tctcctacta agataacttg catattcgtc ggaatcgctt tgaataatgt
    36061 gttagccagc catgtatcga ccatagacat ctcatcaaca atcaaaagtc cgccttctaa
    36121 ttctttcgta ctgactgaag gtgttttttc ccgtccattc agccccagaa gacgatggat
    36181 cgtactggca ggcagacctg tcgtttcgtt cattcgttta gctgctcggc cagttggtgc
    36241 tgcaagtaga ataggaaaaa tttcttgggt atattttgaa ggatccaatt ccagtccgtt
    36301 caactctgca aaaagattga cgattccgtt gatcacagta gttttccccg tacctggtcc
    36361 tcctgttaaa ataaatagag gggaacggat tgcttcagcg atagcttgtt cttgcgaagg
    36421 tccatactgt atgccgaata ttttttcgat acgccgaata ttcttctgca cttctttttc
    36481 aggataatgg atttcttttt tacgactgag aaggcgctgg atcgaattac cgatccccca
    36541 ttcagaaaag tacaaactgt tttcgtaaag attagtctct tcttgttgta tctttccttc
    36601 ttcaacaagt tggataatta cagtagcaac ctgatcaggc tggatttcaa ttgggcgact
    36661 tgtttctaaa atacggatcg tttgctgaag caatgtctcc gccggtacgt atgtatctcc
    36721 tgtttgaact gcatgctgaa aaatctgatg caagatagct gctcgaattc ttctcggtga
    36781 atctgctgca ataccgattt gttcggctag attatctgca cgtttgaacc cgattccttc
    36841 tatgtcttcg actagctggt acggattttc ttcgatgata tccaaagact cgttcttata
    36901 ggcctggtag atactgaaag ctaactgact accgaacccg taacgattca gtcccatgat
    36961 cacttgatcc atcccgtggt ttagccgaat cgtctcagca agcatttctc tttttggctt
    37021 cgtcaaaccg gagatttgtt ttaataccga aggatcagca atgatttttt cgatagcatc
    37081 ttctcccagc aattctacaa ttttttctgc tgttttcttc ccgattcctg ggaatttttc
    37141 gctggaaaga tacgctacga tcccatttgc agttgttggc ttttcttgct catatctttc
    37201 gagtttaagc tgccttccgt atttaggatg atccactaat tctccataaa aacgataagt
    37261 ctcattttct tgaatatcgc cgaaacttcc ggtgactaca atctcttttt cacggtaatc
    37321 agctgtggtt tcactgatgt gtaccaaaag cactttgtaa aaattactag gattttgaaa
    37381 aaaaatcgcg gcaactgtac caacgaaata aggcatttct tctggctgca tcggaagccg
    37441 cctccttctt ctatagtaaa ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt
    37501 tccatacttt cagttcatat ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca
    37561 aactactatt gaacaatcca cccatttcac gcaactcact tagctctttt cctgtcatcc
    37621 attgaatggc tgcagttaaa gaagctccat gtcctacgaa taaaagtggg ccatctttat
    37681 gttgggcaac cgcatctgct acaacagttt cgatacgtgt gattgccgat tcaatcgttt
    37741 ctccgtgaaa aggagaagga tcatacagat ctaaatgatt acgcaaattc ggcagattat
    37801 ccggatattt ctggtacatt tcatcgatcg actgaccttc taaagtaccc agccctagtt
    37861 ctcttagctt atctgtataa aaaatctcta ccggacgagc aagctctttg ttgatacctt
    37921 ctgctgtatc acgtgctcgc tttgctgtac tggaataaat tgctttaaaa ggaacatatt
    37981 ttaccttttt cccaaatgct ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc
    38041 cattgctccc ttgaaaacgt ctttcctgat tccattgtgt cttcccatgt cgggtaaaat
    38101 atagttccat acgcagcaaa ctcctgtctt tcttttattt tactctataa taaactgtct
    38161 aatccagctt gtcattttca tagtgatgcg tcaattctaa attagggaat ccttgctggc
    38221 gaagtgcttc atacactatc attgcagctg tattcgataa attcaatgag cggacatgct
    38281 catcattcat agggatgcgt aaacactttt cttcgttttc acgcataaat gtttctggca
    38341 aaccggttgt ttcttttcca aataaaaaga agtgctcttc tccatcagca aaattttcgt
    38401 cactatatac tttatgggca aattttgaga tcaaatgtag tttacgatta cctaaaaatg
    38461 ttaaaaaagc ttctaaatta ctgtggtaag taatatttac atcattccaa taatccaacc
    38521 ctgcccgttt tagatgtttg tcatcagttg aaaaacctag tggctcgatt aaatgaagcg
    38581 gagtattcgt ggctgcacac gtacgggcaa tatttccagt gtttgcaggg attaatggtt
    38641 caaataaaac gatatgattc atcaggatca ttcctctcat atttatgttc gtttctattt
    38701 tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag
    38761 tcgttacgtt agtgaagctc ttctcactaa cttttatcag aacaggaacc tgataaaaac
    38821 caatgaaaaa acaacttgtt gctaatataa caccctcaac ttttttttgc aacctctact
    38881 cattaaaaac tgctagaaaa acgttttctc ttagttcctt ttaataattc tatgtcaatt
    38941 gtaatcttct tcaaaggatt cttatataag tctgctttga ttcgctcttc tacctgccat
    39001 tctaaaggtg acatggccaa aagatctttt tgattttcct ctggtatctc aaattcatag
    39061 gttacacgct gacgttcagt taatttcatt gcttcagcaa acttggctac tacacgttca
    39121 ttggaataac tctgtttttc aggttgattc ggataaaaag cctgacgtag ttcctttaaa
    39181 taattttctt gtggcaccac cttcaacact tctccatctt ctgagagtac acgttggaat
    39241 tcttggtaat gagaaggcga aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac
    39301 ggcaaattgg tcaaatcagc tacacaccaa aatgcttcgg cagattgttc tgtagctgcg
    39361 tagactcctt cttttgaaat atcaaatcca acagcatttt gaatcttttt caattttgaa
    39421 agttcattca agaagctacc ttctccgcag ccaacatcta atagcgagac ttcttcaggt
    39481 aaatgctgcg caatagtctc aataacgggc tgatacatgc cagattggat cattctacgc
    39541 cttggaacga acattccttg atcgtattct gttttaaccg catgggataa aaaataaagt
    39601 gttccttttt tggaaaggtc aaaacggtga ttatttttgc atatcaagcc attatcagac
    39661 ttttttactt cttcttggca aaccggacag cggaacagct gatgattttc tgccaaaaac
    39721 tggcgtcctc ggtcaatttt ttttaacata gttccccctc atttctgttt tatcttaccg
    39781 ttagatgctc actttggcaa ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag
    39841 ataaaggaga agattagatg atcaaagaat tttgcgctga aaactacaca tctattcctc
    39901 tagcaattgc tagaggagcc gatcggatcg aattgtgtga caacttagcg gtcggtggca
    39961 caaccccgag tactggtgtg atcgaagaaa cgttaagtta tgctggggaa aaagatatcc
    40021 ccgtaatgac gattattcgt cctagagggg gcgacttcgt ctataacgat atcgaattga
    40081 aaatcatgca tactgatttg attgaagcaa agaaattagg gacggatggc gtagtatttg
    40141 gttgtcttac tccttcaggc tggctagacg aagaggcctt agaactattg atcgaaacag
    40201 cagaagggtt gcagatcacc ttccatatgg cttttgacag tattcctgaa gatcggcaat
    40261 tcgaagcaat cgattggctg gtagaacatg gtgttgaccg tatcttgact catggcggtc
    40321 ctgctgacaa aactattgta gaaaatttcc cacggcttaa agaattgatt gcctatacag
    40381 atggccgctt aatcattttg cctggtggtg gtgtcactgc agagaatgca gcacatgtag
    40441 ctaaagaatt gaacgtaagc gaagtacacg gaaccaaaat cgtggatctt cagttataat
    40501 cttataaaca aaaaaacatt tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt
    40561 ttacataaaa tagcccataa ataaagaagc aatattgaaa taaatcagta cactcgtcaa
    40621 atcgctcaaa gtcgtgataa agggaccgct tgctactgct ggatcaaatc ctagacgatc
    40681 cattagcatc gggattaaac tacctgcaag attcgccact gtgattgcaa atagcatagc
    40741 catccctatg acaaacccca agataaaatt atgtttccaa aaacctacga ttagaaaaat
    40801 cgtgatcccc gtaacagctc ctgtgacaac gccggtcaag acttcagcca aaacaagtcg
    40861 aacaaatcca tgatctttct catcagaaat cgcaagtcga cgaacagcca cagctagcga
    40921 ctgggttcca gcattaccgg cagttcctgt aatcagcgaa atgaagactg ctaaaatact
    40981 cgcttcactt accaagtctt catagtgact gattaaagaa gcggtcgaca tacctaaaaa
    41041 caacagagtg atcagccaag gcaaacgtcg agacgctgcc tttactggat tttcattaat
    41101 ttcttccaca ttgaccccgg caagtcctga ataatcgcta gcagcttcat catcaataac
    41161 gtcgatgata tcatcgactg tgacgattcc taacaaatga tcatcataat cggtaacagg
    41221 aagagctaag aagtcataat ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac
    41281 atgaactgaa agcactcgtt cgctcatcag atcagaaatc attgcgtcgt catcattcac
    41341 gattaggtcg cgcagagaaa taacccctac tagctgacct tcttgattaa cgacatagat
    41401 gtaatagatt gtttcagcta catctgcttc gttcttcagc acgtacatcg cagagcgaac
    41461 agtttgattg gctacgatcg aaacaaattc agttgtcatg atcgcgccag ccgtttcatc
    41521 ctcgtaatgg agaagttcct tgatttcact ggcatcgtct gccgacatca aacttaaata
    41581 tttagcaatt tgctttttat ccagcgtatt aagcaagtca acagcattat ctgtatacat
    41641 ctcagaaagc atatcagctg catagctagg acgcatttca tctaggtatt ctttcatatg
    41701 ttcgttgtct tcttcaatca catcgaacat atccgctaat tctttaggcg acagatagga
    41761 atagatcagc tgcctgttct cttctgtaag agattggtag aattgcccct gctcatagat
    41821 atgcatctct aaaaaactgt ttctgaatgc ctgaatatta ttttccctta aatcttgagt
    41881 caactgggca aatctttcct ctaattcttg attttcatcc aaaccaatct cacctcgcct
    41941 tattcttcaa accgtctcac aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc
    42001 agcaactctt ctgtaaatgg atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt
    42061 tttattccca gatccattcg cccgccatac atatcatcac cgagcagggc acagccaatt
    42121 gcggaaaaat gaactcttat ttgatgtgtc cttcctgtat gcagctggat atcaactaac
    42181 gcgatttctg ttagtcgctt ctctatccag tactcagtct gtgccgaacg tccttcatca
    42241 gagaccattc gcttcaatag cgaagaataa tctcgggcga tcggcaaatc gatatgtcca
    42301 tgtgccgata aatctgaaag ccttccactg actaaggcct gatatttttt cacaaacttc
    42361 ttttcacgaa gttgcatatc taattttgcg tgcgcaaatc catgctttgc aaaaagcatc
    42421 aaaccagatg tatcccgatc tagtcgcgtc accacatgga ttacttggtt ttcgtaacct
    42481 tgttttttat aataagcctt tacacgatta gccattgtcc catttggatg gtattgggca
    42541 ggaatagacg aaacgcctgc tggtttattt actaccagaa cgtgttcatc ttcatacacg
    42601 atatcgatcg gtgtttcatc caaaagtacg gtttcatgtt ctccttcagc aggaatcaca
    42661 atggttacct tatcatcctt agctagagaa aacaagacat tttctacttg atcattaact
    42721 ttgatttgac cgccttgaaa ttttattttc gctaacagac cctttgagat ccctttttct
    42781 tttaaaaaat attttacttg ctgagaatga tctttctcat aatcccatgt aaactgcata
    42841 tcaatcctca ctaataaagg catctttcac acgatgccaa aaatgcatat gtcgataaga
    42901 agcaaaatgg atccgttctt ctgctattcg ataataaatt gagcggatat ttgcttttgc
    42961 gacattcaat tgatcaaccg ttacaagata atcttctgta ttttccaatt ttatttctag
    43021 ccattcatca tgggcaatca cgatcggaga acccaaagta cgaaacactc gattattcag
    43081 tgaagcaatc tctgccaact gaaaggcatt gatactagga tggatcacag caccgccgac
    43141 acttttatta tatgctgtcg aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa
    43201 actttcaaac agctcgtttt tgatgtatac atcggctacc atcgtacgat tggcccgttt
    43261 aatcgttgat tcattcagtg ctaaaaaatg ctggtccggt gtttcatcca aataactgat
    43321 ccgaacatcc agcaagggat aactaacact ttgttcccgg ttcgttcgta agctgtctac
    43381 tagttcttcc agctcgtaat cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac
    43441 gcccaagaaa cgcacatcat tcagacaatg actgtagtga tggaacgccg acaataatgt
    43501 gccgtctccg ccgactgaga tgaccaattc tggctcatga tcatctcgtt gaatccctgc
    43561 ttgttctaaa agtgcgttca aacgttctgt cacttctata gtctttgctt ctttattatg
    43621 aacgatcgct actttcatcc cctgcctcct ctctttcgct ttctttcttc aaaagcgttc
    43681 gatagtagac atcatctgat tttcctcttc catgggagaa taaccgctgt gcttcttgga
    43741 tttcttcacg gatactggac atctcttcat ccaactgata ggcagcttct gccgcacgct
    43801 gaagtcgttc actcatttct tctggaaaaa cgccttgata tttataattc agcgaatgct
    43861 caatcgttgc ccagaaattc atcgcaagag tccgaatctg gatttccgcc aaaattttct
    43921 tctcaccgct gatcagctgt acaggatact cgatcaccag atgataagaa cggtagccac
    43981 ttgctttttt atttgtgatg taatctcgtt cttggagaac tttcatgtct tttcttttac
    44041 ggatgatctc aacaacttga tggatatctt ctacaaactg acacatgatc cttaacccgg
    44101 caatatcaga catttcttct tctaaacgtc ccattggaat atgccgtaat tgtgattttg
    44161 ttacaatact atcgactggc ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat
    44221 tttgttcacg gaactgtttg cggatccccc gcagtttcac tttcaattct gatacagttt
    44281 gttcatatgg tgctaaaaat gcttcccaat ctcgttccat agctgatacc tctacttcat
    44341 taacattcat tgtttattct accatatatg caaagttttt tcgtactctt gatttatgca
    44401 ttgaccaaaa aaagaaaatt aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa
    44461 tgcttgaaat cgaatataaa tctatgctga caaagcaaga gtacgagata ttcatcgctc
    44521 actatcaatt aacagataaa gatttccgtg tacagactaa tatttatttc gataccgttg
    44581 atgaacagct gaagaaacaa aattgtggcc tacggatccg tttcgttggt catcaagctg
    44641 aatatacatt aaaaacacct gctgaaaaag ggcgtctcga aacaacggat acctttataa
    44701 cagaagaagc agaagcattt atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc
    44761 aaaaactttc tgatttgaat atcgatcctg cttcattaat ccaaaccgga aagcttacaa
    44821 caaaacgggc tgaattccct attgaagaag gacttttagc tattgatgaa agctggggag
    44881 acaatctaca cgattttgaa ctggaactag aggtaagggg atgcatctgt gggaaaaatt
    44941 gcttttataa attttttaaa acgtttttct ttaccttatc gtcctgctaa gaacaagatc
    45001 caacgtatgc ttgaagctaa aaattagaaa cctttcattt tcacaaggca cttgagttca
    45061 tttttttact tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata
    45121 gaatctattt tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg
    45181 gaatactgtt tcgaaatgga acaacagtta ttacaattta tcgaagaaga gtacgggaca
    45241 ccatcaaaag aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattggg
    45301 gatgtcatgc aacaaaaagg aatttctcag aacaatcttg aggaacgtaa tcacttattc
    45361 tcaatgactt attctgcagc attggactgc aaagcggcgc agtttcaagg aaagaaaaaa
    45421 ggccgccgtt ttttgatcaa attgcaagaa gcagtcggct gtaatgacat accatattct
    45481 caagaacttg ctgaatccat atttgaagaa atcggcggcg atttgaatat gtttaaagaa
    45541 gatcgtcagt ctgacttcgt taaagaagta ttcttttctg atcaaaaaat tgctcgggaa
    45601 atgggcatca caaaacaccc ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt
    45661 ggtgttttag tagaagtaaa aggtacaagt agcctttgga gaatcacaga gctgtgtaaa
    45721 acaacagata actcatatca agtttttaat ggtgaacatt tacatacaaa tgattctcag
    45781 ttccattcaa gaactagcca tttacacttg ctttctaatt aaaaaaatac gtcgactccc
    45841 ttacaagaga gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc
    45901 ttataaacga tcgatcaatt cttctaattc atttagacgt tgttcaaaca tcgacattgc
    45961 atcttcaata tatgcagcct gtgtcatatc aactcctgct tttttcatta cttccacagg
    46021 ataatcgctg ttacctgctt ttaggtaagc caagtagttc tctagtgcct ctggttcctg
    46081 gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt attggaaaac
    46141 atagtaatta taataaaaat gcggtatccg tgaccattcg aatttaattt ctgggtcttc
    46201 ttcgactgct ggaccataat attttgcatt caatttacca taactatcac ttaggtattc
    46261 actagtcaat ggcacacctt tttcatcttc cgtatgcatg aaatgctcga attcagcgaa
    46321 ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag taatggttga gtacataagc
    46381 ccgtacacga gggtcttttt ctgtttccaa taaatactcc gttaggatat tttcattcgt
    46441 tgtcgaagca atttctgcta aaaagatgga gtagtcgccg tacacataag gctggttcga
    46501 acgagtgaaa taactatgaa cactatgtcc catttcgtgg acaagcgtaa atagctgatc
    46561 cagcgtatca tgccaattca ataaaatata tggatttgtg tcatagcttc ccgaagaata
    46621 agcaccgctt cgtttccctt tattttcgac aacatcgatc caacgttcag agaatgcttt
    46681 ttctacgatg gccatgtatt cttcacccat tggtttcagt gcttctaaag ctttttcttt
    46741 tgcttcttcg tacgtaaagg taattggagc ttcccctaag accggtgtat aaagatcata
    46801 catgtgcagt ttttccactt ctaataaacg tttccgtaat tccatgtatc gatgtaacaa
    46861 aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat acactttcag gaatatgatt
    46921 attgctcaaa gacgcttctc tggcagagct gtaattacgg actttcgctt taaaattatg
    46981 tccttttata tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt cgtaaacact
    47041 gtacaatccc ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc
    47101 atacacgcca tgagataatt ggactatttc accattttct ccttcaatcg ttggaaaaac
    47161 tagatctgca ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc
    47221 tccagcaaga agagattcct gctcagcaga taagacatgg gctcgattat ctactatttg
    47281 ctggatataa tggcgataga cttccaattt tggttcttct ttgaaatatt gccaaatctg
    47341 gtcatctgac aattgcaata tttctggttc aaaccaggaa acggcttcac taactttcga
    47401 aaacagactg cttgctcttg cataaagcgc ttggtaatct gtatttccag tgtcttgatc
    47461 gtttttaagg tgcgcatata cataaatgac ttcagtttgg cgataaaccc tcaatacgaa
    47521 ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga
    47581 ttgttttaac tcttctgata attccaaata tttctcatca aactcttggt cgcttgaaaa
    47641 gatcttggtc aagtcccaag ttaaattttc aggcaattct tctcgttttg gcaactgctt
    47701 tacttccatg aaatccactc cttttcttcc cttagctcta tctatcaaaa tagatcatag
    47761 tctaacatcc acatgtcaca gcatctaact tttctactca ttcatgtgtc tgcttccatc
    47821 aattttagca taactaaatc ttgttttaca tagaaaaccg attcctagcc tatttttata
    47881 cttactattt tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc tgatagacca
    47941 tctcttcatc aatcatagga aaaagccatt cccttttatg ttcttttatt tggcaaagcc
    48001 atgagttgaa atcaggaact ttctggtttt ccaaggcttc ttcgtacaac attcgataaa
    48061 gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt ttgatagatc cacgatgata
    48121 gatgcaaaag atgtttatga cgcaagtagc attgttcttg tatggatact gtattctttt
    48181 gcttgtgaat aagttggcgt gtcagccatt tcttcccatc ttcactcact ttttctattt
    48241 ttttcatttg gttcatttta atttcgttac tagtgaatat ttctttaagg tccctactag
    48301 aaaaagtcca gcagctactg caaaaggaaa tatgccctga tacagtctct gtaatatgat
    48361 gatacaaata aagcttttgt ttcttgagat cagctttcca gcaatgcact cctctttttc
    48421 tattatagta gcagcaactt ttttctatca atgagaattg cgattgtcca agaaaagaat
    48481 taccaagtat ccaccatact tggtaactgt tattcaaata attttgggtt ctttcaataa
    48541 atctctggtg agacagtcta gaacattgga tttctattgc tagattcgcc aacaaaatat
    48601 ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa tactggttct actgactgtc
    48661 ccagccaatc catgaagcat tcctttaact gtaagtgctc ttttgattct ctttcactaa
    48721 agaccgaaca gttatctgat ttttgatggg caaaatgagc acattttttc tttccctttt
    48781 tcaaaatcac tctttttcga cagccacgac aataatattt ctttcctttt tctgcatgtt
    48841 tagcaaaaca gcacttatta tattcattca ttgcaagcag catttttctc cctctgaact
    48901 tatttacgtc taagaattta tttacacgaa aaaagagggc gtgaaaaagc tattctgcat
    48961 caagtaataa gaacagctaa acaaaaatag ttcccaatat ttttcgtttg gttgggcttg
    49021 ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg acacaatttt
    49081 tgtcacagtc tcttactata atgctgattt atacaaaata aaatttcgtt cgctcgatag
    49141 cattacgcgc catcagacat tgtccatatt cattcaatgt ttctggtgta cgatcgctct
    49201 tttcagcaaa ctccaaaatt cgagcaatct gattctctac atccgcttgg ttgagatttt
    49261 ccatccagaa gtgaacgact aggtaatagc gctcttcgaa taaatacaaa tcagtccaag
    49321 cagactctag ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa
    49381 aagttgcttg tctttcctgt tcttctaggg cgcttccaga taattcttcc tccggatcgc
    49441 tgccaataat ctgctgacga agaaagtcac tcacatcttc tgaatcaaca gtagaatctt
    49501 ctgattgaga aatttcttca tccggaagat ttttactgat aaacagttct aatccgtctc
    49561 cttttggcaa aacttggaaa gtgacagctt cgcttcctcg gaactcctct tcgatatcaa
    49621 cttcttccaa aatactataa aagaaatttt cgatttcttt gtgattgcca agtaaatcca
    49681 aaaatgaaac gccccgttcc tctaaatctt catgaccaat tagtacacgg atcgtatttt
    49741 cattaatatg ttccatttcc attttgctac acctcacttt gccacaatgt ttaatataga
    49801 ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt
    49861 aataaaacca gcggattttt caacctttca agctatctta tttaactata acatttttgg
    49921 cagacgaata ctatatagcc gacttttcag ctaattttcc ctctctattt ttcgtgcaat
    49981 catcatcttc tactaccttt ttatacatct aatcaagaat actttttgta aaaacaaaaa
    50041 aacttggaaa aactatcttt gtaatagtct tt
//
LOCUS       DAZKOM010000009        48003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000009 DAZKOM010000000
VERSION     DAZKOM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.009"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..602
                     /locus_tag="ACG8NF_000554"
     CDS             231..602
                     /locus_tag="ACG8NF_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0471062.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(876..1568)
                     /locus_tag="ACG8NF_000555"
     CDS             complement(876..1568)
                     /locus_tag="ACG8NF_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0471063.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1571..2356)
                     /locus_tag="ACG8NF_000556"
     CDS             complement(1571..2356)
                     /locus_tag="ACG8NF_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0471064.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2593..3993
                     /gene="lacG"
                     /locus_tag="ACG8NF_000557"
     CDS             2593..3993
                     /gene="lacG"
                     /locus_tag="ACG8NF_000557"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0471065.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4018..4335
                     /locus_tag="ACG8NF_000558"
     CDS             4018..4335
                     /locus_tag="ACG8NF_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0471066.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4348..6021
                     /locus_tag="ACG8NF_000559"
     CDS             4348..6021
                     /locus_tag="ACG8NF_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0471067.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6035..7666
                     /locus_tag="ACG8NF_000560"
     CDS             6035..7666
                     /locus_tag="ACG8NF_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0471068.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8157..9722)
                     /gene="guaA"
                     /locus_tag="ACG8NF_000561"
     CDS             complement(8157..9722)
                     /gene="guaA"
                     /locus_tag="ACG8NF_000561"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0471069.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9781..10704)
                     /gene="coaA"
                     /locus_tag="ACG8NF_000562"
     CDS             complement(9781..10704)
                     /gene="coaA"
                     /locus_tag="ACG8NF_000562"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0471070.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10900..11505
                     /locus_tag="ACG8NF_000563"
     CDS             10900..11505
                     /locus_tag="ACG8NF_000563"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0471071.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11813..12829
                     /locus_tag="ACG8NF_000564"
     CDS             11813..12829
                     /locus_tag="ACG8NF_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0471072.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12970..14271
                     /locus_tag="ACG8NF_000565"
     CDS             12970..14271
                     /locus_tag="ACG8NF_000565"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0471073.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14317..14979
                     /gene="deoC"
                     /locus_tag="ACG8NF_000566"
     CDS             14317..14979
                     /gene="deoC"
                     /locus_tag="ACG8NF_000566"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0471074.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15000..15398
                     /locus_tag="ACG8NF_000567"
     CDS             15000..15398
                     /locus_tag="ACG8NF_000567"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0471075.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15464..16546
                     /locus_tag="ACG8NF_000568"
     CDS             15464..16546
                     /locus_tag="ACG8NF_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0471076.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16758..18320
                     /locus_tag="ACG8NF_000569"
     CDS             16758..18320
                     /locus_tag="ACG8NF_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471077.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18313..19446
                     /locus_tag="ACG8NF_000570"
     CDS             18313..19446
                     /locus_tag="ACG8NF_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471078.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19446..20402
                     /locus_tag="ACG8NF_000571"
     CDS             19446..20402
                     /locus_tag="ACG8NF_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471079.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20501..21667
                     /gene="deoB"
                     /locus_tag="ACG8NF_000572"
     CDS             20501..21667
                     /gene="deoB"
                     /locus_tag="ACG8NF_000572"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0471080.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21691..22509
                     /locus_tag="ACG8NF_000573"
     CDS             21691..22509
                     /locus_tag="ACG8NF_000573"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0471081.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22526..23233
                     /gene="deoD"
                     /locus_tag="ACG8NF_000574"
     CDS             22526..23233
                     /gene="deoD"
                     /locus_tag="ACG8NF_000574"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0471082.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23531..24217)
                     /gene="gpmA"
                     /locus_tag="ACG8NF_000575"
     CDS             complement(23531..24217)
                     /gene="gpmA"
                     /locus_tag="ACG8NF_000575"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0471083.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24389..24970)
                     /locus_tag="ACG8NF_000576"
     CDS             complement(24389..24970)
                     /locus_tag="ACG8NF_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0471084.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25103..25786)
                     /gene="rpiA"
                     /locus_tag="ACG8NF_000577"
     CDS             complement(25103..25786)
                     /gene="rpiA"
                     /locus_tag="ACG8NF_000577"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0471085.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26105..26518
                     /gene="rpsL"
                     /locus_tag="ACG8NF_000578"
     CDS             26105..26518
                     /gene="rpsL"
                     /locus_tag="ACG8NF_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0471086.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26602..27072
                     /gene="rpsG"
                     /locus_tag="ACG8NF_000579"
     CDS             26602..27072
                     /gene="rpsG"
                     /locus_tag="ACG8NF_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0471087.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27166..29253
                     /gene="fusA"
                     /locus_tag="ACG8NF_000580"
     CDS             27166..29253
                     /gene="fusA"
                     /locus_tag="ACG8NF_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0471088.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29425..30612
                     /gene="tuf"
                     /locus_tag="ACG8NF_000581"
     CDS             29425..30612
                     /gene="tuf"
                     /locus_tag="ACG8NF_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0471089.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31019..31327
                     /gene="rpsJ"
                     /locus_tag="ACG8NF_000582"
     CDS             31019..31327
                     /gene="rpsJ"
                     /locus_tag="ACG8NF_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0471090.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31358..31987
                     /gene="rplC"
                     /locus_tag="ACG8NF_000583"
     CDS             31358..31987
                     /gene="rplC"
                     /locus_tag="ACG8NF_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0471091.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32015..32638
                     /gene="rplD"
                     /locus_tag="ACG8NF_000584"
     CDS             32015..32638
                     /gene="rplD"
                     /locus_tag="ACG8NF_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0471092.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32638..32928
                     /gene="rplW"
                     /locus_tag="ACG8NF_000585"
     CDS             32638..32928
                     /gene="rplW"
                     /locus_tag="ACG8NF_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0471093.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32967..33800
                     /gene="rplB"
                     /locus_tag="ACG8NF_000586"
     CDS             32967..33800
                     /gene="rplB"
                     /locus_tag="ACG8NF_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0471094.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33843..34121
                     /gene="rpsS"
                     /locus_tag="ACG8NF_000587"
     CDS             33843..34121
                     /gene="rpsS"
                     /locus_tag="ACG8NF_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0471095.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34144..34491
                     /gene="rplV"
                     /locus_tag="ACG8NF_000588"
     CDS             34144..34491
                     /gene="rplV"
                     /locus_tag="ACG8NF_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0471096.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34505..35161
                     /gene="rpsC"
                     /locus_tag="ACG8NF_000589"
     CDS             34505..35161
                     /gene="rpsC"
                     /locus_tag="ACG8NF_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0471097.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35164..35598
                     /gene="rplP"
                     /locus_tag="ACG8NF_000590"
     CDS             35164..35598
                     /gene="rplP"
                     /locus_tag="ACG8NF_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0471098.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35588..35776
                     /gene="rpmC"
                     /locus_tag="ACG8NF_000591"
     CDS             35588..35776
                     /gene="rpmC"
                     /locus_tag="ACG8NF_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0471099.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35801..36067
                     /gene="rpsQ"
                     /locus_tag="ACG8NF_000592"
     CDS             35801..36067
                     /gene="rpsQ"
                     /locus_tag="ACG8NF_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0471100.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36125..36493
                     /gene="rplN"
                     /locus_tag="ACG8NF_000593"
     CDS             36125..36493
                     /gene="rplN"
                     /locus_tag="ACG8NF_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0471101.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36530..36838
                     /gene="rplX"
                     /locus_tag="ACG8NF_000594"
     CDS             36530..36838
                     /gene="rplX"
                     /locus_tag="ACG8NF_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0471102.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36866..37405
                     /gene="rplE"
                     /locus_tag="ACG8NF_000595"
     CDS             36866..37405
                     /gene="rplE"
                     /locus_tag="ACG8NF_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0471103.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37424..37609
                     /locus_tag="ACG8NF_000596"
     CDS             37424..37609
                     /locus_tag="ACG8NF_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0471104.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37645..38043
                     /gene="rpsH"
                     /locus_tag="ACG8NF_000597"
     CDS             37645..38043
                     /gene="rpsH"
                     /locus_tag="ACG8NF_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0471105.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38075..38611
                     /gene="rplF"
                     /locus_tag="ACG8NF_000598"
     CDS             38075..38611
                     /gene="rplF"
                     /locus_tag="ACG8NF_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0471106.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38772..39128
                     /gene="rplR"
                     /locus_tag="ACG8NF_000599"
     CDS             38772..39128
                     /gene="rplR"
                     /locus_tag="ACG8NF_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0471107.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39149..39649
                     /gene="rpsE"
                     /locus_tag="ACG8NF_000600"
     CDS             39149..39649
                     /gene="rpsE"
                     /locus_tag="ACG8NF_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0471108.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39664..39843
                     /gene="rpmD"
                     /locus_tag="ACG8NF_000601"
     CDS             39664..39843
                     /gene="rpmD"
                     /locus_tag="ACG8NF_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0471109.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39887..40327
                     /gene="rplO"
                     /locus_tag="ACG8NF_000602"
     CDS             39887..40327
                     /gene="rplO"
                     /locus_tag="ACG8NF_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0471110.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40327..41622
                     /gene="secY"
                     /locus_tag="ACG8NF_000603"
     CDS             40327..41622
                     /gene="secY"
                     /locus_tag="ACG8NF_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0471111.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41684..42331
                     /locus_tag="ACG8NF_000604"
     CDS             41684..42331
                     /locus_tag="ACG8NF_000604"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0471112.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42522..42740
                     /gene="infA"
                     /locus_tag="ACG8NF_000605"
     CDS             42522..42740
                     /gene="infA"
                     /locus_tag="ACG8NF_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0471113.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42773..42889
                     /gene="rpmJ"
                     /locus_tag="ACG8NF_000606"
     CDS             42773..42889
                     /gene="rpmJ"
                     /locus_tag="ACG8NF_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0471114.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42907..43272
                     /gene="rpsM"
                     /locus_tag="ACG8NF_000607"
     CDS             42907..43272
                     /gene="rpsM"
                     /locus_tag="ACG8NF_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0471115.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43300..43689
                     /gene="rpsK"
                     /locus_tag="ACG8NF_000608"
     CDS             43300..43689
                     /gene="rpsK"
                     /locus_tag="ACG8NF_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0471116.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43769..44707
                     /locus_tag="ACG8NF_000609"
     CDS             43769..44707
                     /locus_tag="ACG8NF_000609"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0471117.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44735..45115
                     /gene="rplQ"
                     /locus_tag="ACG8NF_000610"
     CDS             44735..45115
                     /gene="rplQ"
                     /locus_tag="ACG8NF_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0471118.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45387..46226
                     /locus_tag="ACG8NF_000611"
     CDS             45387..46226
                     /locus_tag="ACG8NF_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0471119.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46202..47071
                     /locus_tag="ACG8NF_000612"
     CDS             46202..47071
                     /locus_tag="ACG8NF_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0471120.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47061..47858
                     /locus_tag="ACG8NF_000613"
     CDS             47061..47858
                     /locus_tag="ACG8NF_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0471121.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15178 a         8774 c        10519 g        13532 t
ORIGIN      
        1 tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata atatttttga
       61 tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt tggtttttaa
      121 tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa gttggttacc
      181 ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata atgagagatt
      241 acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat gaattatctg
      301 ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac tatattgata
      361 tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata aaagctttta
      421 tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta gtaggaaaat
      481 taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct ttgtctatgt
      541 tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg gaaatctttt
      601 aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat gatttttaag
      661 attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga tagagatgaa
      721 cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt tcactaagga
      781 tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat
      841 ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga tgtggcagac
      901 aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt tagctgtgcc
      961 tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt tccgatcccc
     1021 gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca atcagccgga
     1081 gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt cccagggaaa
     1141 gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc gaaaatatca
     1201 aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg aatctgttgg
     1261 tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc gttttcattt
     1321 tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc gatctcagag
     1381 gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg accatgaata
     1441 ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat catatgcagc
     1501 caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc aaatataatc
     1561 cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt tgcagatcga
     1621 catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta atgagcagat
     1681 cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt ttattatcaa
     1741 cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct ccttcttcgg
     1801 tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat gcttttgtaa
     1861 actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg gtatttttcc
     1921 ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga atatcaaaaa
     1981 ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc gccaatagtt
     2041 cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag gaggctgctg
     2101 tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg tgagaaagtt
     2161 cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc agcttgcccg
     2221 atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct aattgattga
     2281 tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata gtaagcaatc
     2341 tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat ataaacggac
     2401 aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg tttgttacaa
     2461 tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt gttgacaaat
     2521 gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt atagatttga
     2581 ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc cacagcagcc
     2641 tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt
     2701 ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca tcaatatgca
     2761 aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc cattgcttgg
     2821 agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc tttttatcat
     2881 cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt
     2941 gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac aatagaagca
     3001 tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt gtggagtacc
     3061 tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt cccaccagga
     3121 atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc
     3181 cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt agttcactca
     3241 ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc ctttttagac
     3301 gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta ttcaactgaa
     3361 accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa
     3421 gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg gatcaatcac
     3481 taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca taacggtacg
     3541 ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat ttataaagaa
     3601 ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata tgacttgttg
     3661 ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga gaacgggatg
     3721 gggtataaag atcagtttga agatggcatt attatggatc agccgagaat cgactatttg
     3781 agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt gaaggggtat
     3841 tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa aaggtatggc
     3901 ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc ttactggtac
     3961 aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga agaaaagatg
     4021 aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg cgacgctcgc
     4081 agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca tgttgattcg
     4141 gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac aaaaatttta
     4201 gcggaagaag catctgggaa agagatggag atgggattca tttttatcca tgggcaggat
     4261 catttaatga ccactttatt gctgagagat ctgattcaag actttattgt actgtacaga
     4321 cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg
     4381 aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg tgacggattt
     4441 ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc tgcggttcct
     4501 gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa agtctataat
     4561 tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg cttggctgat
     4621 tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc
     4681 tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt ttcaacggaa
     4741 tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac agttaattta
     4801 tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt tcctggaacg
     4861 atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc ggccgtttta
     4921 attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat cattacggtg
     4981 ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat ctggggagcg
     5041 atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg
     5101 gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct
     5161 tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg aactggggca
     5221 acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt gaaggcagtt
     5281 gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct atttgcggca
     5341 cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt tgtaaacgtt
     5401 tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta cgttttgcct
     5461 tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat cttagctttt
     5521 gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt ttgcaaagca
     5581 tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa
     5641 ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt tatcagcgat
     5701 gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag tgggatgtta
     5761 gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt
     5821 gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt agcaccgcaa
     5881 gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat caagctggtt
     5941 gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc tgttcagttt
     6001 attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt ggttgcgtaa
     6061 agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc
     6121 atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata tggaaaaatc
     6181 tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt taaaaaataa
     6241 tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta atggagaaag
     6301 ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac gagcaaaaaa
     6361 tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg atcctgggaa
     6421 acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata cggcattgta
     6481 cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc ctgatatggt
     6541 tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt cttggggaga
     6601 aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc aagctgttcg
     6661 agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg gcgatacagg
     6721 agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg atttaattgg
     6781 agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca atatgaatgc
     6841 gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg ggcatacaac
     6901 agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg cggggggata
     6961 tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc ggattcaggc
     7021 agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt acaacggaaa
     7081 tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca ctatgggaaa
     7141 cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc
     7201 gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg aaagcgatgg
     7261 accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg ctaagggtac
     7321 gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt ggcaggatca
     7381 agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa cttacacatt
     7441 gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata tcaaaaatta
     7501 tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa aaattacaat
     7561 caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca aggcttcagg
     7621 aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg cggatcgttt
     7681 cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca ggaatcgaac
     7741 ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt taccaaaacg
     7801 agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc aacgatttcg
     7861 taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa
     7921 gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat agaaaaaagt
     7981 tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga aacaaattaa
     8041 cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa tcaagcattt
     8101 tagctattga agaatgttga tttataggga ttccccctcc atattatgtt ggctctttat
     8161 tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg
     8221 gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca
     8281 aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa
     8341 gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc
     8401 cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg
     8461 cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga
     8521 ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca
     8581 cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct
     8641 ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg
     8701 ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt
     8761 tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt
     8821 tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt gactttgatg
     8881 atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt
     8941 agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga
     9001 accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca
     9061 acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct
     9121 tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga
     9181 cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca
     9241 atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag
     9301 tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga
     9361 tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa
     9421 ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct
     9481 aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg
     9541 ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg
     9601 ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac
     9661 tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc
     9721 aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg
     9781 ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat
     9841 atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt
     9901 ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata
     9961 ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca
    10021 tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac
    10081 gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc
    10141 aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac
    10201 aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc
    10261 catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg
    10321 atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg
    10381 agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc
    10441 gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa
    10501 gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat
    10561 atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc
    10621 ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg
    10681 gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt
    10741 ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa
    10801 tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga
    10861 tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct
    10921 gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc
    10981 tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt
    11041 gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg
    11101 ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa
    11161 atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa
    11221 atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat
    11281 gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg
    11341 acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa
    11401 caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc
    11461 acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag
    11521 tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt
    11581 gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag
    11641 agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag
    11701 tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc
    11761 ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct
    11821 gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt
    11881 gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa
    11941 tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac
    12001 catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt
    12061 agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca
    12121 agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct
    12181 ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat
    12241 gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa
    12301 ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat
    12361 cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg
    12421 tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt
    12481 gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc
    12541 tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg
    12601 caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata
    12661 catgattccg caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc
    12721 acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga
    12781 aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat
    12841 attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag
    12901 tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag
    12961 gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg
    13021 aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa
    13081 gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac
    13141 ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata
    13201 aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc
    13261 ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca
    13321 ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa
    13381 gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg
    13441 gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt
    13501 ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt
    13561 ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg
    13621 gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca
    13681 gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc
    13741 gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc
    13801 caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa
    13861 gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg
    13921 ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag
    13981 cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct
    14041 gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga
    14101 ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc
    14161 tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt
    14221 ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa
    14281 gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac
    14341 catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca
    14401 aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa
    14461 caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac
    14521 acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc
    14581 gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat
    14641 atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca
    14701 tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct
    14761 gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt
    14821 ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac
    14881 gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga
    14941 gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca
    15001 tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg
    15061 ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc
    15121 aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt
    15181 ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca
    15241 cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc
    15301 ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg
    15361 cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg
    15421 actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt
    15481 atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa
    15541 cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat
    15601 cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga
    15661 aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc
    15721 ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac
    15781 gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac
    15841 aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc
    15901 aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga
    15961 aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc
    16021 tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt
    16081 gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat
    16141 gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt
    16201 ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga
    16261 ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt
    16321 gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga
    16381 cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct
    16441 aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat
    16501 catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag
    16561 ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta
    16621 tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc
    16681 aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt
    16741 taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca
    16801 aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa
    16861 ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg
    16921 gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa
    16981 gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg
    17041 atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc
    17101 ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag
    17161 tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt
    17221 taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga
    17281 cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt
    17341 cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag
    17401 ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat
    17461 tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc
    17521 ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag
    17581 ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg
    17641 atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg
    17701 gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac
    17761 atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat
    17821 tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc
    17881 tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa
    17941 caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta
    18001 tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac
    18061 tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca
    18121 aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg
    18181 ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg
    18241 aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg
    18301 gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt
    18361 tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc
    18421 atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa
    18481 acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca
    18541 gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc
    18601 tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc
    18661 ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt
    18721 ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca
    18781 tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg
    18841 atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc
    18901 gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag
    18961 aaaacgactt taggatttga aatccgttct gttggattga atccttttgc ttctgagtat
    19021 gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca
    19081 gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact
    19141 tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc
    19201 ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag
    19261 acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt
    19321 gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa
    19381 gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa
    19441 ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa
    19501 caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg
    19561 tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga
    19621 tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag
    19681 gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta
    19741 tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat
    19801 ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag
    19861 gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt
    19921 attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct
    19981 tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat
    20041 atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg
    20101 tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca
    20161 tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat
    20221 tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt
    20281 cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg
    20341 ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat
    20401 aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg
    20461 ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct
    20521 gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt
    20581 tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga
    20641 gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca
    20701 aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg
    20761 ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga
    20821 tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta
    20881 tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat
    20941 tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt
    21001 agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat
    21061 gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag
    21121 caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga
    21181 agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat
    21241 cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat
    21301 gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg
    21361 tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc
    21421 agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa
    21481 cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag
    21541 taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac
    21601 gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt
    21661 aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa
    21721 acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt
    21781 tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag
    21841 atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct
    21901 ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc
    21961 atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt
    22021 gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt
    22081 accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg
    22141 ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa
    22201 gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga
    22261 ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta
    22321 gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga
    22381 atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt
    22441 gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca
    22501 ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat
    22561 cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt
    22621 tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata
    22681 taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta
    22741 tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg
    22801 tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag
    22861 ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa
    22921 tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa
    22981 tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga
    23041 acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata
    23101 ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac
    23161 aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc
    23221 gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa
    23281 gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt
    23341 tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa
    23401 tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga gaagttggca
    23461 ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag tccctttttt
    23521 atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt aattcataaa
    23581 caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag ataccttcga
    23641 tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca gttttgttgt
    23701 ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct aatgtcactt
    23761 tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga cggtcatttg
    23821 ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa gaacgacgcc
    23881 agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc aaaccttgta
    23941 atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc caaagttgat
    24001 ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca gaagtgtaag
    24061 caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt gcttcttcga
    24121 ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat gcattccatt
    24181 cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct tttaagatag
    24241 gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga agagatttcc
    24301 atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa aaacgaacag
    24361 tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt tctttttcag
    24421 gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa gaaataaatt
    24481 ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt tttgcatcga
    24541 tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca actaatgtac
    24601 gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg aaatagattt
    24661 ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc ccgcgatctt
    24721 tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga tgaagttggc
    24781 aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa tgaacagcag
    24841 tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa ccaaagtcag
    24901 ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt gcataaccaa
    24961 taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa cagtaaaaat
    25021 gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt ctttttttat
    25081 tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc tcaacgcctt
    25141 tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt tctactaccc
    25201 caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact tctagatgca
    25261 gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg tctgtacgca
    25321 agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag ccataaggga
    25381 tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa gaatcaacga
    25441 tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa gcagcgccgc
    25501 cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt gtcaaatcga
    25561 ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct tgttcttttg
    25621 tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata cggcgaccaa
    25681 tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc atcccgtcct
    25741 ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt gcgtccacct
    25801 ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg gtaaaaagat
    25861 accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct tgttgacatg
    25921 ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg tgaagaaacg
    25981 tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt attttttatc
    26041 acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa ggaggaaaca
    26101 aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg tggaaaaatc
    26161 taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa ctaacgtaaa
    26221 ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta aaaaaccgaa
    26281 ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag ttactgctta
    26341 tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac gcggtggtcg
    26401 tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag atactgctgg
    26461 tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta aagcttaatt
    26521 ttaattatta aacaaatgaa acttgcttct actatataga agtaattact atgaacaata
    26581 tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg tgatgtttta
    26641 ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt aatggttgac
    26701 ggaaaacgcg ggattgcagc taacattatc tataatgcct ttgacttgat caaagaatct
    26761 acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca acctgttctt
    26821 gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga agttcgccct
    26881 gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt gcgcggtgaa
    26941 cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa cacaggtgct
    27001 tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc gtttgctcac
    27061 tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc agatagtgtc
    27121 cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac cagagaattt
    27181 tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg taaaacgact
    27241 gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga aactcatgaa
    27301 ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac gatcacttct
    27361 gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac accaggacac
    27421 gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc tgtaactgtt
    27481 cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca agcaacaact
    27541 tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc tgacttctta
    27601 tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat ccaattacca
    27661 attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa ggctgaaatg
    27721 tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga ataccgcgaa
    27781 ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga tgaagaactt
    27841 actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga aggtatccgc
    27901 cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt taagaacaaa
    27961 ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt agatatccca
    28021 gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc agacgacagc
    28081 gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg tcgtttaact
    28141 ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa cgcaacaaaa
    28201 ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg ttcagaaatc
    28261 tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac aactactggg
    28321 gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt cccagaacca
    28381 gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat gggtgtggct
    28441 ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt ggaaacaggc
    28501 gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga ccgtatgcgt
    28561 cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg tgaaactttc
    28621 cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa aggtcaatac
    28681 ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga atttgaaaat
    28741 gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac tggtttgaaa
    28801 gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa agctaaactt
    28861 tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt tgctgcttct
    28921 atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc aatcatggct
    28981 gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt aactgctcgt
    29041 cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt tcgtgcgatg
    29101 gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac tcaaggtcgt
    29161 ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt acaagaagaa
    29221 atcattaaga aaaacggcgg aaaagctgaa taatttcttg aaaccggata gtcctgcaaa
    29281 gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat tatcgatgat
    29341 atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa aaaatatatt
    29401 gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc taaaccacac
    29461 gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac agctgcaatc
    29521 acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca aatcgatggt
    29581 gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga gtatgaaaca
    29641 gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt taaaaacatg
    29701 atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc tgacggccca
    29761 atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc ttacatcgtt
    29821 gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt agttgaaatg
    29881 gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc tgtagttgct
    29941 ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat tcttgaatta
    30001 atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa accattcatg
    30061 atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac aggtcgtgtt
    30121 gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc tgaagaaact
    30181 tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta cgctgaagct
    30241 ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca acgtggacaa
    30301 gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga agtatacgtg
    30361 ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg tccacaattc
    30421 tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac tgaaatggtt
    30481 atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc tatcgaaaac
    30541 ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt cgttacagaa
    30601 atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga cgtaatcgct
    30661 cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag gatcaggaaa
    30721 aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta ttttctaaat
    30781 gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt gagtttatat
    30841 atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa tcagaatgat
    30901 ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac gaacgagcgt
    30961 gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag ggaacaagat
    31021 ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt tagatcaatc
    31081 agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg gtccaattcc
    31141 attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat acaaagattc
    31201 acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga acccaacacc
    31261 aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa
    31321 attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa tcttagggaa
    31381 aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag taactgttgt
    31441 agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg gctacgaagc
    31501 tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg cgaaaggtca
    31561 tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa atgttgagct
    31621 aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag gagacattat
    31681 tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac gccacggaca
    31741 aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt caatgggtcc
    31801 agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg
    31861 cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat
    31921 caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag ctgtgaaagc
    31981 taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat tattcaaaca
    32041 agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa ttgaacctaa
    32101 tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac aaggaacaca
    32161 cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc gtcaaaaagg
    32221 aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag gtggcgtagt
    32281 ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc gtcgtttagc
    32341 aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt
    32401 gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact tatctatcga
    32461 ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat cagcacgtaa
    32521 cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg ttgtatcaaa
    32581 tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc ttgcataatg
    32641 aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct tgccatggat
    32701 gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt aaaacaagcg
    32761 gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa
    32821 ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca
    32881 ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc actaatagat
    32941 aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg
    33001 gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca cctgaaaaat
    33061 cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc atcactgtac
    33121 gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag
    33181 ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca gctaacatcg
    33241 cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa ggactagaag
    33301 tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat gctcttccat
    33361 tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct ggtaaaggtg
    33421 gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg
    33481 tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa
    33541 tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct ggacgctctc
    33601 gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat gatcacccac
    33661 acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct ccttggggtc
    33721 aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa cttattgttc
    33781 gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag aggaggttca
    33841 ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg
    33901 aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag
    33961 ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa catgtaccag
    34021 tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct acaagaactt
    34081 atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg aggataaatc
    34141 caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg cacttcacct
    34201 cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca
    34261 atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt gatgtcagca
    34321 gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt aagcgaagct
    34381 tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct
    34441 ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata aggagggata
    34501 atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc gcgactggga
    34561 tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt taagaatccg
    34621 taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc
    34681 aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta ttggtaaagg
    34741 cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta aaagaatcca
    34801 tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag gtgaaggaat
    34861 cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg
    34921 ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc taaacggagc
    34981 agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata ctttacgtgc
    35041 ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag gagttaaagt
    35101 gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag gagggaaata
    35161 atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg
    35221 gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc tgttgattca
    35281 cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg ttacatgaaa
    35341 cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc caaagcaatt
    35401 ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc agtaaaacgt
    35461 ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt
    35521 ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga aatgggtggt
    35581 gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc ttgatcaaga
    35641 aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag gtcaattaga
    35701 aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg
    35761 tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag gaagaaatca
    35821 acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc
    35881 tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt ctaagaaata
    35941 caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa ttatggaaac
    36001 tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag aagcagttat
    36061 tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa ggaggataca
    36121 caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat
    36181 tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat
    36241 cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa
    36301 agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat atattaaatt
    36361 tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa cacgtatctt
    36421 tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt cactagcacc
    36481 agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta tgtttgttaa
    36541 aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag gcgttgtact
    36601 agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca
    36661 ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag cgccaattca
    36721 tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg gctacaaaga
    36781 agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag ataaataaaa
    36841 gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt
    36901 aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac ctaaagttga
    36961 aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa acttagataa
    37021 agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa aagctaaaaa
    37081 atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg
    37141 cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac cacgtgtacg
    37201 tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata ctttaggtat
    37261 taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag tacgtggtat
    37321 ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca
    37381 attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta aaaaatcaat
    37441 gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg
    37501 ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt gcttccgcga
    37561 acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa aagtaaattc
    37621 gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga ttttctaact
    37681 cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc atcaaaaatt
    37741 aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc
    37801 gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt
    37861 gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt caaagctgac
    37921 gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt
    37981 gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg
    38041 taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt
    38101 tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag ttaaaggacc
    38161 aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg aaggaaacga
    38221 agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg gaacaactcg
    38281 tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact
    38341 aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga acgttggtta
    38401 ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac cagctaacac
    38461 acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg caaacattcg
    38521 tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg gcgaatatgt
    38581 acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat agaagccagc
    38641 agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc
    38701 gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat aaaaataaag
    38761 aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag agacatcgtc
    38821 gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt tttcgttcta
    38881 acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta gcaagtgcct
    38941 ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa gctgtcggta
    39001 aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt gaccgtggtg
    39061 gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa aatggactag
    39121 aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac atttggaatt
    39181 agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct
    39241 acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtaggat ttggtacagg
    39301 taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga agaaaaactt
    39361 agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg cttttagtgg
    39421 tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg gtggaccagt
    39481 tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt taggctcaaa
    39541 tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga
    39601 agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag gaggacaaca
    39661 ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa
    39721 cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt taagccttct
    39781 aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt tgaagaaatt
    39841 taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga aacttcatga
    39901 attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc
    39961 tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg
    40021 tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac caaaacgagg
    40081 attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat tgaaccgctt
    40141 tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga
    40201 aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc
    40261 agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat caatcgaggt
    40321 gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag atcgaaaatc
    40381 ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac tgtaccaggc
    40441 gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat gttgaacttg
    40501 gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc accatacatt
    40561 actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg
    40621 tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta
    40681 gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata cgctcaatta
    40741 ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat tctaacagcc
    40801 ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat cggaaatggc
    40861 gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat cagagaaatt
    40921 tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc tatttttgtg
    40981 gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt ccaacaagct
    41041 gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac aagcagctat
    41101 ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag ttcgtttatt
    41161 gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga aaactggtac
    41221 aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat ttatacgatt
    41281 ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga gaaattagcg
    41341 gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa ggggactgaa
    41401 gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt ccttggttta
    41461 gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc aatcggatta
    41521 ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa acagttagaa
    41581 ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt gttgaggttt
    41641 cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc tcattttaat
    41701 ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa atcattgatg cttatggtat
    41761 tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa cagctcttgg
    41821 cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag taacaaatgg
    41881 aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat tagatggctt
    41941 tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa
    42001 aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg
    42061 ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc ctacaaaagt
    42121 tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag atgataaacc
    42181 tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa tcttggctta
    42241 ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg atgctgtatt
    42301 tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga cttgcattat
    42361 gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg acttctagag
    42421 aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt ccgccatttt
    42481 atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa gaagatatga
    42541 ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag
    42601 aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat tacatccgta
    42661 tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac cgtggccgta
    42721 ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta tcatgaaagt
    42781 aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt
    42841 tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat tggaggtgct
    42901 ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt agtagtttca
    42961 cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc tgatgctggt
    43021 gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc tatccgtgca
    43081 gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa
    43141 cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt
    43201 ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc
    43261 aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat
    43321 cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca ttctacattc
    43381 aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg gtcatcagct
    43441 ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca aatggcagca
    43501 gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt aacagttaaa
    43561 ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg tttagaagtg
    43621 actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc aaaacgccgt
    43681 cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga acaagacact
    43741 tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa gaatcgcaaa
    43801 aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa gagggtatgg
    43861 gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag gtgctgccat
    43921 cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag gtgttagaga
    43981 agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt atggccaaga
    44041 agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg gcgatattat
    44101 cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg tctctgaagg
    44161 agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc aagcagatga
    44221 aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct acacacctgt
    44281 tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag attttgataa
    44341 attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa tgagtttgtc
    44401 tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg aagcaaaaaa
    44461 tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat
    44521 cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag gaatcaatac
    44581 tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta acttaggacg
    44641 taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga
    44701 agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac taggacgcac
    44761 atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa tcaacgaacg
    44821 tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa aaatgatcac
    44881 tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga
    44941 agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt tacaaaaact
    45001 gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac gcattttgaa
    45061 gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg tctaaaatca
    45121 cactaataac atcactttat agatgattct tttagagtgt tatgatgttg gaagatattt
    45181 ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt aagcactcat
    45241 cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt gaaggcataa
    45301 aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag tgttagtagg
    45361 aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa aagataaatt
    45421 ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt acaattgata
    45481 aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca cttgccaaaa
    45541 cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat caaatattgg
    45601 atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa aatccagaca
    45661 atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa aatcaaggga
    45721 ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt agaatggctg
    45781 agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt gttgccattg
    45841 ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc agtatgctag
    45901 atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat
    45961 tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat cggatactag
    46021 tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt tctgctggtc
    46081 ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag agcgcactga
    46141 aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg gtggattggt
    46201 tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac catttgaaca
    46261 aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg cattagtagg
    46321 gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg ttaagccaac
    46381 aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata ataagaattt
    46441 aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac agctattcga
    46501 agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga
    46561 agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa gctatttgga
    46621 acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg ctggtgtgct
    46681 tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg atccccaggg
    46741 cagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga tcactattgt
    46801 attagttacg catttaatgg atgatgtagc caattttgca gattatgtct atgtgttgga
    46861 aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct
    46921 aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat taatggctaa
    46981 agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg ctatcgtcca
    47041 ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat atgcctggag
    47101 attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt tattttatcg
    47161 gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt ttcacattac
    47221 tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt cgcccattga
    47281 tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc ggagaaactt
    47341 attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc ctctttattt
    47401 tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact acaccgcctt
    47461 tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta cggttcccag
    47521 tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg
    47581 aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc
    47641 tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc agcagcttca
    47701 accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt ggggaaggaa
    47761 gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc attgcttttg
    47821 tattattaac tgtgctactg atctttttaa gaggataaac gttcagtggg ccgctatcta
    47881 ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac aagaaatatt
    47941 agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag aaagaacgaa
    48001 aaa
//
LOCUS       DAZKOM010000010        44880 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000010 DAZKOM010000000
VERSION     DAZKOM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.010"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8NF_000614"
     CDS             212..604
                     /locus_tag="ACG8NF_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0471122.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8NF_000615"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8NF_000615"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0471123.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8NF_000616"
     CDS             2698..2943
                     /locus_tag="ACG8NF_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471124.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8NF_000617"
     CDS             3165..4775
                     /locus_tag="ACG8NF_000617"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0471125.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8NF_000618"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8NF_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471126.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8NF_000619"
     CDS             5067..5936
                     /locus_tag="ACG8NF_000619"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0471127.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8NF_000620"
     CDS             6101..7372
                     /locus_tag="ACG8NF_000620"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0471128.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8NF_000621"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8NF_000621"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0471129.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8NF_000622"
     CDS             8763..9026
                     /locus_tag="ACG8NF_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0471130.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8NF_000623"
     CDS             9471..9920
                     /locus_tag="ACG8NF_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0471131.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8NF_000624"
     CDS             9977..11395
                     /locus_tag="ACG8NF_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0471132.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8NF_000625"
     CDS             11395..12255
                     /locus_tag="ACG8NF_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0471133.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8NF_000626"
     CDS             12429..13202
                     /locus_tag="ACG8NF_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0471134.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8NF_000627"
     CDS             13349..13714
                     /locus_tag="ACG8NF_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0471135.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8NF_000628"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8NF_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0471136.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8NF_000629"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8NF_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471137.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8NF_000630"
     CDS             15560..16216
                     /locus_tag="ACG8NF_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0471138.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8NF_000631"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8NF_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0471139.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8NF_000632"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8NF_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0471140.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8NF_000633"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8NF_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0471141.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8NF_000634"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8NF_000634"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0471142.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8NF_000635"
     CDS             21888..22202
                     /locus_tag="ACG8NF_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0471143.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8NF_000636"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8NF_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0471144.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8NF_000637"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8NF_000637"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0471145.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8NF_000638"
     CDS             23546..23875
                     /locus_tag="ACG8NF_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0471146.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8NF_000639"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8NF_000639"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0471147.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8NF_000640"
     CDS             24863..25687
                     /locus_tag="ACG8NF_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0471148.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8NF_000641"
     CDS             25680..26027
                     /locus_tag="ACG8NF_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0471149.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8NF_000642"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8NF_000642"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0471150.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8NF_000643"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8NF_000643"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0471151.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8NF_000644"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8NF_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0471152.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8NF_000645"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8NF_000645"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0471153.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8NF_000646"
     CDS             31684..32451
                     /locus_tag="ACG8NF_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471154.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8NF_000647"
     CDS             32441..34483
                     /locus_tag="ACG8NF_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471155.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8NF_000648"
     CDS             34764..34913
                     /locus_tag="ACG8NF_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0471156.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8NF_000649"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8NF_000649"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0471157.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8NF_000650"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8NF_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0471158.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8NF_000651"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8NF_000651"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0471159.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8NF_000652"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8NF_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0471160.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8NF_000653"
     CDS             39330..40412
                     /locus_tag="ACG8NF_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0471161.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8NF_000654"
     CDS             40412..41215
                     /locus_tag="ACG8NF_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0471162.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8NF_000655"
     CDS             41285..42025
                     /locus_tag="ACG8NF_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0471163.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8NF_000656"
     CDS             42036..43040
                     /locus_tag="ACG8NF_000656"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0471164.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44878)
                     /locus_tag="ACG8NF_000657"
     CDS             complement(43085..44878)
                     /locus_tag="ACG8NF_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471165.1"
                     /translation="MKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYT
                     KDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKIL
                     YQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLIS
                     FIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSE
                     DILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVL
                     LYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHC
                     LIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLL
                     EQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCS
                     FCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETH
                     AIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDV
                     ADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14426 a         7832 c         9343 g        13279 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
//
LOCUS       DAZKOM010000011        44870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000011 DAZKOM010000000
VERSION     DAZKOM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.011"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1054)
                     /locus_tag="ACG8NF_000658"
     CDS             complement(44..1054)
                     /locus_tag="ACG8NF_000658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0471166.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1234..1578)
                     /locus_tag="ACG8NF_000659"
     CDS             complement(1234..1578)
                     /locus_tag="ACG8NF_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0471167.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1571..2005)
                     /locus_tag="ACG8NF_000660"
     CDS             complement(1571..2005)
                     /locus_tag="ACG8NF_000660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0471168.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2143..2880
                     /locus_tag="ACG8NF_000661"
     CDS             2143..2880
                     /locus_tag="ACG8NF_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471169.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2873..4084
                     /locus_tag="ACG8NF_000662"
     CDS             2873..4084
                     /locus_tag="ACG8NF_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471170.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4288..5070
                     /locus_tag="ACG8NF_000663"
     CDS             4288..5070
                     /locus_tag="ACG8NF_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0471171.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5140..5802
                     /gene="trmB"
                     /locus_tag="ACG8NF_000664"
     CDS             5140..5802
                     /gene="trmB"
                     /locus_tag="ACG8NF_000664"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0471172.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACG8NF_000665"
     CDS             complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACG8NF_000665"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0471173.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7786..8715
                     /gene="comGA"
                     /locus_tag="ACG8NF_000666"
     CDS             7786..8715
                     /gene="comGA"
                     /locus_tag="ACG8NF_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0471174.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8672..9721
                     /gene="comGB"
                     /locus_tag="ACG8NF_000667"
     CDS             8672..9721
                     /gene="comGB"
                     /locus_tag="ACG8NF_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0471175.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9718..9996
                     /gene="comGC"
                     /locus_tag="ACG8NF_000668"
     CDS             9718..9996
                     /gene="comGC"
                     /locus_tag="ACG8NF_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0471176.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9971..10441
                     /gene="comGD"
                     /locus_tag="ACG8NF_000669"
     CDS             9971..10441
                     /gene="comGD"
                     /locus_tag="ACG8NF_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0471177.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10413..10760
                     /locus_tag="ACG8NF_000670"
     CDS             10413..10760
                     /locus_tag="ACG8NF_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471178.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10723..11148
                     /gene="comGF"
                     /locus_tag="ACG8NF_000671"
     CDS             10723..11148
                     /gene="comGF"
                     /locus_tag="ACG8NF_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0471179.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11120..11479
                     /gene="comGG"
                     /locus_tag="ACG8NF_000672"
     CDS             11120..11479
                     /gene="comGG"
                     /locus_tag="ACG8NF_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0471180.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11651..12658
                     /locus_tag="ACG8NF_000673"
     CDS             11651..12658
                     /locus_tag="ACG8NF_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0471181.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12683..13867
                     /locus_tag="ACG8NF_000674"
     CDS             12683..13867
                     /locus_tag="ACG8NF_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0471182.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACG8NF_000675"
     CDS             complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACG8NF_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0471183.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15992..16120)
                     /locus_tag="ACG8NF_000676"
     CDS             complement(15992..16120)
                     /locus_tag="ACG8NF_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471184.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16465..16608)
                     /locus_tag="ACG8NF_000677"
     CDS             complement(16465..16608)
                     /locus_tag="ACG8NF_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0471185.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16831..17310)
                     /locus_tag="ACG8NF_000678"
     CDS             complement(16831..17310)
                     /locus_tag="ACG8NF_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0471186.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17383..17667)
                     /locus_tag="ACG8NF_000679"
     CDS             complement(17383..17667)
                     /locus_tag="ACG8NF_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471187.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17745..17939)
                     /gene="ssrS"
                     /locus_tag="ACG8NF_000680"
     ncRNA           complement(17745..17939)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8NF_000680"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18011..19297)
                     /locus_tag="ACG8NF_000681"
     CDS             complement(18011..19297)
                     /locus_tag="ACG8NF_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0471188.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19435..20106
                     /locus_tag="ACG8NF_000682"
     CDS             19435..20106
                     /locus_tag="ACG8NF_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0471189.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20072..20560
                     /locus_tag="ACG8NF_000683"
     CDS             20072..20560
                     /locus_tag="ACG8NF_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0471190.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20573..21520
                     /gene="prmA"
                     /locus_tag="ACG8NF_000684"
     CDS             20573..21520
                     /gene="prmA"
                     /locus_tag="ACG8NF_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0471191.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21521..22285
                     /locus_tag="ACG8NF_000685"
     CDS             21521..22285
                     /locus_tag="ACG8NF_000685"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0471192.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22518..24731
                     /locus_tag="ACG8NF_000686"
     CDS             22518..24731
                     /locus_tag="ACG8NF_000686"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0471193.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24742..25188
                     /gene="dtd"
                     /locus_tag="ACG8NF_000687"
     CDS             24742..25188
                     /gene="dtd"
                     /locus_tag="ACG8NF_000687"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0471194.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25359..26618
                     /locus_tag="ACG8NF_000688"
     CDS             25359..26618
                     /locus_tag="ACG8NF_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0471195.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26707..27654)
                     /locus_tag="ACG8NF_000689"
     CDS             complement(26707..27654)
                     /locus_tag="ACG8NF_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0471196.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28048..29340
                     /gene="hisS"
                     /locus_tag="ACG8NF_000690"
     CDS             28048..29340
                     /gene="hisS"
                     /locus_tag="ACG8NF_000690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0471197.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29355..31124
                     /gene="aspS"
                     /locus_tag="ACG8NF_000691"
     CDS             29355..31124
                     /gene="aspS"
                     /locus_tag="ACG8NF_000691"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0471198.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31233..32108
                     /locus_tag="ACG8NF_000692"
     CDS             31233..32108
                     /locus_tag="ACG8NF_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0471199.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32374..34257
                     /locus_tag="ACG8NF_000693"
     CDS             32374..34257
                     /locus_tag="ACG8NF_000693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0471200.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34332..34616)
                     /locus_tag="ACG8NF_000694"
     CDS             complement(34332..34616)
                     /locus_tag="ACG8NF_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0471201.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34606..35340)
                     /locus_tag="ACG8NF_000695"
     CDS             complement(34606..35340)
                     /locus_tag="ACG8NF_000695"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0471202.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35469..36092
                     /locus_tag="ACG8NF_000696"
     CDS             35469..36092
                     /locus_tag="ACG8NF_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0471203.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36284..37405
                     /locus_tag="ACG8NF_000697"
     CDS             36284..37405
                     /locus_tag="ACG8NF_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0471204.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37412..40540
                     /locus_tag="ACG8NF_000698"
     CDS             37412..40540
                     /locus_tag="ACG8NF_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0471205.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40559..43753
                     /locus_tag="ACG8NF_000699"
     CDS             40559..43753
                     /locus_tag="ACG8NF_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0471206.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43899..44606)
                     /locus_tag="ACG8NF_000700"
     CDS             complement(43899..44606)
                     /locus_tag="ACG8NF_000700"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0471207.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14394 a         7966 c         9413 g        13097 t
ORIGIN      
        1 acaaaaaaag cgagacataa gtcttcgctt tttttgtttt cttttatttt gatgaatcag
       61 ttgtttcttc agttgaagat tcttttgttg aatcagttga ttttgtgtca gaactctttg
      121 ttgaagctgt agtttcactt gagtctttcg tacttgatga agttgttgtg aatgcactca
      181 acacattttc aaaggcatca tctttgattt ttacatttgc atcttttaat tcttcaccga
      241 ttactttagt tgtgaatgca ttgtcactta gtttagtttc agtagcgata tcttttagtt
      301 gatctttgta tttatccatg tcattgcctt tattttggtt tttcaccatt ttgacaacgt
      361 agtattctgt cgcataagaa gtaggatttg ttgtagtaat aacatcggag atctcaccgt
      421 ctttcaattt gaaagctgct tcttttactt ctgcaggaat agttgttgtc gttgagtcga
      481 atttaacttt accgccatct tcttttgtct ctgtatcagt tgatttgtct ttggctaatt
      541 tagagaaatc atctccatca tccgctgatt ttttcacatc ttttgcttca tcttcacttg
      601 ataatttgat gatttgtgct tctacttctg gatggaaaga tttccaagca gttttcaaat
      661 cgtcatctgt aatatctacg tgagctttca agccagcttc aaaagctaaa ttgttacgaa
      721 tatattcttt ataagtatct ttcgtatatc cagcagcttc taattgtgat tcaaaagtat
      781 cacctaatga tttcgcttgt ttgtcgtatt ctgcatccac ttgcttatcg gttactttat
      841 cgccatattt gttattgaat actttataaa tgatcatccg ttgtactaag gattggttcg
      901 atgattcaag ttttgcttca tcatagaaat cagatacagt gatcgttccg cctttcatcg
      961 ttgcgatatc tttattagta tcgcctgaac aagctgctag agttaaaaca gccaacgcac
     1021 tagtagctgc caaaataatt gatttttttt tcattaaaat ttgcactcct gtaattagtt
     1081 attattttta tatattaact ataccatatc ccatttcctt taaaaacgtt gtcgtggtag
     1141 tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa tacgcaaact tttctcactt
     1201 atatagttaa atctgttgag aaaataatca ttattatatt tgttcaggat actctggcag
     1261 atcagctttt agtttttcta cttgttcatt gatttgtgtg atacgtggtt cggcttggaa
     1321 tttaaaatct tcgatatctt ttttgattgc ttcttggaaa gcaggaatca cagattcagc
     1381 tgtttctttt gtttcgatca atgcgctttt aaaatgattc aggctgttat ttaaatcatt
     1441 tgtcagagtt gttgcttctt ccaattcttc gatcagtttc tttcttgttt cattcccgct
     1501 cctgggcgcc aataacagac ctccgacagt gccagcagca gctccaaata acagtccttt
     1561 gaaaaagttt ttaatcatcg caggacacct gctttacgat tgtttcttga atagctttta
     1621 gttcttccga tccataacta tcttgatgat tgacaaagtg aatagaaaaa tcatcctctt
     1681 tgctgtagcg agggactaaa tggatatgtg aatgaaaaac agattgatag gctagttcac
     1741 ggttgttatt caagatatta agacctttca tttctggaaa agctttttcc aatgcttgag
     1801 caatcttagg cacacgcgaa aaaacctctc ctgcaagttc gggatcatat tcaaaaatat
     1861 ctgctacatg acgtttaggc acaactaatg tgtgtccttt cgttgcttga gagatgtcta
     1921 aaaaagcata gaccttgtcg tcttcatata ttttatagct tgggatttct tgattgatga
     1981 ttttgcaaaa aatacagttt tccatataaa acatcctttc tataaagaat aaatacttca
     2041 gcttaacttt accataagtt aaagctattg taaggaaaaa aacaaaatac cgtcattttc
     2101 ttcgtggtat aataaataaa aataaatggc ggaggaaaaa gaatgagttt aattatcgat
     2161 catgtcacag gggggtacgg acatctccct gtgttaaaag atatcagttt cgatgtacag
     2221 cctggagaga tagtagggtt gattggactg aacggagcag ggaaaagtac gaccatcaag
     2281 cacattatcg gtctgctgga agcttcaaaa ggaacgatag aaatcaatca tctatcctta
     2341 aagaaaaata cggaagaata tcgcaaacaa attgggtata ttccagagac tccttcttta
     2401 tatgaagaat tgacattgag agaacatatc gaagtcactg cgatggccta cgatatacct
     2461 atggatatag caatgaaaag agcagaaaag ttgttggaga cttttcggtt atctaatcgt
     2521 cttgactggt tcccagctaa tttttcaaag ggaatgaaac agaaagtgat gatcttatgc
     2581 gcctttttga tcgagccttc tttatatatc atcgacgaac catttttagg cttggatcca
     2641 ctagctatca atgcattgct tgaattgatg gtagaaatga aaaaagaagg agccggtatt
     2701 ttgatgtcta cgcatatttt agctactgca gaaaaatatt gtgatcgttt tgtggtctta
     2761 cataacggag agatacgagc agacggttct cttgagatgt tgagaaaaga atttcagctg
     2821 ccggattctt ctttggacga aatctatatt gctttaacaa aggagcaggc acatgagtag
     2881 tttttttcaa aaacgtttag caagacatca aaagaaaatg atgaaatata tgcgttatgt
     2941 attgaacgat cattttgttt tagtttgtct tttcttgatt ggcggtgtgg gattatatta
     3001 ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt ggagggctca taatctctgt
     3061 attttggatg ctctgtttat tcgtgggtaa gatggctact tttgcagagg cagcagatat
     3121 tgttttttta cttcctaaag aaaaagaaat gcaaacctat ttggaacgag cttttcgcta
     3181 ttcttgcatt tttccgttga tctttttgat attggcatgc ggattggcca tgccattagt
     3241 ggtggttagt acggagcaat ccttttcgat gtttttcgtc tatctgatca tattatggag
     3301 tttgaaattc agtcatctgc aaatcaagag agcagaattg tttcgactag atcaacaagt
     3361 tattcgttat tggaaattag gctggttttt ttcttctctg atctttttgt ttctatcatt
     3421 ttattggcta atatgggcag gaatagcagg tgcattagta caggcaagtc tatattactg
     3481 gtttttgtgg aagcaacaag aaaaaggatt ggattgggag aaaatggtgc atgtagaaga
     3541 acataggttg catcagatct atcaatttat caatttattc acagatgttc ctgaaattac
     3601 ggcaaagatc aaaagaagac gctatttgga tcctatattg actcatatta aaagagaatc
     3661 aaaaaacact tatctttact tgtttgcaag aagattggca agaggttctg attttagtgg
     3721 tctttatttc cgattagtaa ttataggtgg gttattgatt gccggagtag tcgactttta
     3781 ttttagtctg attatcggtt tcttattttt atatttgatc ggtttccaac tgttaccttt
     3841 agcgaaccag ttccgctata tggttctcgt tcagctttat ccaatccata agaatcagaa
     3901 gaagcaggcg attcagtatc tactcacatg ggtattaatt atcgcgtcgg ttatttttgg
     3961 tttgattgct gcagtcgtat tagatgggca gcagcgatgg gttccgctag cggcttattt
     4021 gattgttact atgctctttg ttaaactata tgtaccaact cgtttgaaga agatgtcaga
     4081 ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa atagagcgtt gacagtaaga
     4141 atgctgttcg ttctattttt tttgagaaaa gctggttttt tgaatggcaa ttaaatgaaa
     4201 aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact agaataataa gtactgcaaa
     4261 acaaagagta agaggacggg agaatgcatg atggagtttc agttagacca agagtggcgg
     4321 ttacgtccca tcaaaggcga cacaggaaaa acatacatag gcatgcgtga tgaggacaaa
     4381 gtctttatta aacgaaacac gactccgatg ttggctgctc tttcaaggga aggaattaca
     4441 ccaaaacttg tttggacaaa acgaacgggg aatggcgata ctttgacagc acaagagtgg
     4501 ctggaaggtc gcttgctgac tcctaatgag attggtcaaa gaaacgacgt gattgatgtt
     4561 ttgtaccatt tacatcattc aaattctctt aaaaccatgc tgacccgtat cggtggtcgt
     4621 gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag aattaccgga agaactccag
     4681 aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg agcagatccc gtcaatagat
     4741 aaggaaaact tatcagtagt acacggagat gtaaaccatc gtaattggtt agtatgtaaa
     4801 aattatttat atctagtcga ttgggattca atcatgtttg cagaccctgc ggtggatatt
     4861 gcaacgattt taggaaatta tgtgccactg tctagctgga atcagtggct gatcagttat
     4921 ggcatacgcc cgactgacga aatgcttgaa aaatttcatt ggtatgcggc catgaatttg
     4981 ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc gacggatgaa cgaagaaatt
     5041 ttacaattaa aacgaatttt cagtggctga ggtttggccc aaactctgcg gagatttggc
     5101 cagacctttt taattagagg aaaggaatgg agataaataa tgcgtgtccg aaatcgtccg
     5161 ggagcagcgg aaatgttagc ggctcacccg aattttgtaa ttagtgatcc aacattgtgg
     5221 aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa tccacattga gattggtatg
     5281 gggaaaggcc agttcatcac tggaatggca aaagcccatc ctgaaatcaa ttatattgga
     5341 gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca agttgatcga acaaccgctt
     5401 cctaacctga aacttctaca tgtggatgga tcagcgttga cagagtattt cgctgacagc
     5461 gaagtagatc aaatctatct gaatttttcc gatccatggc caaaaaaacg tcatgaaaaa
     5521 cggcgattga catataagac ttttcttgct gtagatgaac agattcttcg tccaaatgga
     5581 gaaatccatt tcaaaacaga taatcaagga ttgtttgagt attctctggc aagtttttct
     5641 caatatggca tgatcctcaa acaggtatgg ttagatttac atcaaagtca atttgaaggc
     5701 aatattatga cggagtatga agaaaaattt tcttcaaaag gtcaacgtat ttatcgagta
     5761 gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt gaaactaact ttctgctttc
     5821 ataaaaacat taatatccat aaataaatcc cagaaaatga acattacaga tgtaatgtat
     5881 tttctgggat ttatttgatt taaaatgtat ttatttcatc ggtttttcca caaggtggaa
     5941 tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt gatcttcaat tcgggtatga
     6001 cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa ggttttagct gctcctagtt
     6061 gatgtgcttt agttattaat tcattcaact gttcatagac gatcgaaaaa ggttcctgtg
     6121 tcatgattcc gcctatagga agagaaacac aagctatttc ctgctgtttg ttgacaacgg
     6181 tcatcccgcc acctttttga atcaaaagat tagcagcata cagcatatcc tcgtcgtttg
     6241 tcccaacgac aactagatta tgcgagtcat gggcaatcgt tgtcgctatc gctccttgtt
     6301 tcagctgaaa tcctttcact atgcctaatc caatgtttcc cgtttgatgg tgacgctcga
     6361 tcaccgcaat ctttaattgg tctttttcga ttgaaggaat gaatcgttcg ccttgtcggt
     6421 ccactttttc taccaaatct ttagtcacta gactattcgg gataatttcg atcacatggg
     6481 cttgtgaaga agaaagaagc agctctaacg aatgtggatt taatggttga gcgtttagtt
     6541 ttggcagaca acacgtatat gaatttgaat gatcagcaaa taaatggtct tggagttgcc
     6601 cagattgaac aactaatgtt cccttataga aaacttcatg aattggcagc ttggtaagat
     6661 cgtctgttaa gaagaaatca gcttgatacc caggggcaat cgcacctagt gttttcaaat
     6721 caaaacattc tgctgcattc aatgttgcca tctgaatggc ttgtaatgga tcgattccaa
     6781 gtgcaatagc ttgacggacg atatgatcga ttgatccttc ttttactaaa tcattgatca
     6841 gtttatcatc ggtgacaaag aagcatcttc ttgaattttc aggtgtgatc gccggtagta
     6901 gcgatgccag atcttttgca acggtccctt ctcgaaccat taaatacata ccaagatcca
     6961 aacgttcttt agcttctttt gcagttgtag cttcatgatc cgtccgaata cctgctgcag
     7021 gataaacatt taactcttcc atgccgatac cagctgcatg cccgtcaatt tttttcttat
     7081 gctggtgcat caattggatc ttttctagga tgtctgtttc attatttgct actgcttgat
     7141 agttcatgac ttcagctaag cctaaaactt cgggtttttg taaaaaaggg gctaattttt
     7201 ttgcatttaa atttgcacca ttggtctcaa aaggggttac aggtacacaa gaaggtagca
     7261 taaaaaagat attcatgagt gtttgtcttg catcttctat cataaactca agacctgtac
     7321 tcccagcaat gtttcctatt tcatggggat ctgtcactac agtagtaact ccatgaagca
     7381 aggatacttt tgaaaattcg cttggtgata aaagtgagct ttcgatatgc acatggccat
     7441 cgatgaatcc tggcactaga tattgcccat tagcattatg tacgcttttt ccttcgtatt
     7501 caccgattcc tacgatcatt ccttcgcaga ttgctacatc tttttccatg atttctttcg
     7561 taaagacgtt gacgatctta gcatgcttga tcactaggtc agcaggcttt tttttacttg
     7621 ctacttcgat atatttcctt aatgttgttt ccaatggagt gcctcccaag ataaattttt
     7681 ataagtataa cataaattcg gtttttgcca atattttttt gtaaattcat tttatttgaa
     7741 acaaaaagat caaaaagacg tatgtttttt gtggagggga gaataatgga aataaaacga
     7801 ttatcggatc aaatgataag tgaaagtttt gccaaacatg cccaagacgt atatatattg
     7861 ccagcttcag aagtatttta catctatttt cgctatggaa aaaaacgaac attctggaag
     7921 aaagtctctt cggcagatgg acagcaactg atctcaagat ttaagtattt gggacagatg
     7981 gatatcggag aaaaaagaaa agctcagtta ggtgcattta cttattcttt agaaaaacag
     8041 gaagttcgtc tgcggttgtc ttctgtagga gattaccttc aacgagagag tttagtgatt
     8101 cggctattat acggtacttt tgaaacaccc catcgttgct ttagttctcg agattttgca
     8161 actattttac agaaaaccgt cagacgtgga ttataccttt tcagcggacc agtcggatca
     8221 ggcaagactt ctctaatgta tcggttggct ttggaagaaa aattgcaagt gattactatc
     8281 gaagatcctg tagagataga agaaccgttt tttctgcagc tacagatcaa tccgaaaata
     8341 gcacagactt atgatacact tttgagactc gcattacggc atcgtccaga cttgctaatc
     8401 attggcgaga taagagatga atcaactgca catgctgcgg taagagcagc actaacaggg
     8461 caccgggttt ttgcaaccgt acatgcacgc aatcttgaag gtacattggt cagaatccat
     8521 gaactgacaa agaaaaaaga agagctatct gaatgtttag ctggtgtcat ttatcaagaa
     8581 cttttggaag atgccaatca ccatccttct gttttatgga gttatcaatt tatggaggaa
     8641 gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag ctcaggaaaa tatttggcag
     8701 tcagaactat tctaaaggat taacaagaag ccagcaaaac cagtttgcac aaaccatggg
     8761 agaactgctt ttatctggtt ttagtctgca agaatccctt tatttattac tcacgatccg
     8821 ttcgtttcca cctgttattt tggcaaatat ccaacatcag ctaaaggaag ggcaagcgtt
     8881 tagtgaggta ctttcgcaaa taggatatgg agcagagcaa ctggtacaga tcgagttagc
     8941 tgaacgtcat gggaatctgc cgacaacttt gttggaaatt gccaaacagt ttcggcttgt
     9001 tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat ccgatgctat tgctcacctt
     9061 tttgctaggt atcttgttta gtatgcggtt gttcgttttg cctcagctta ttggttcagg
     9121 aatggtcatg caagagcatt ggggtattca gctgatacaa gtctttcatt ggtatgttct
     9181 actattcatc ctagcggtta ctctattggc tttactagta aggtatcgtc tgatgaagtt
     9241 gtctcctgtt gatcagagtg aatggataag cagacaatta ttgattggac cgatatattc
     9301 cttgtatcaa tcggcttatt tgtcactaga gattggaaag ctttttcatg aaggtttcga
     9361 attaaggcaa atcatagtct gcttaaagga aacgaaacaa gaaagtctga ttcagcatct
     9421 tgcctctaag ttgattcaag gattggaggc aggtatgtca ttggctgagc agttccagaa
     9481 ttatcgtttt ctatcagaag aattcagtaa aattgtttta cagggagagg caaagggaag
     9541 tttaggtaaa gaactgattt tttacagcga atggacaaga aaacagcttt tcaaacgtct
     9601 tcatcactgg atgcatttta ttcagccaat catttttttg ggcgtagcgg ctttgatttt
     9661 attagtttat gccgctgttt tattacctgt ttacggaagc atagaggagg ttttgccatg
     9721 aaaaagagaa aaatatctta ttatagcggt tttacattga tcgaaatgct aatcgtctta
     9781 ctgatcatca gtgtattagt gttgttgttc gtccctaatt tgtcgcgtta taggaatcat
     9841 gtagaccaag aaagtcgtga agcgatcatc cagttggtag acacacaaaa agagctctat
     9901 gcgcttcaaa ataatggaag agttcctact gtggaggaat tgttgaatga aggctatatc
     9961 aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt aaggttttag catcaggata
    10021 tacgttgttt gaatcactct tactgatttt attacttaca acgatccttt cttttccagt
    10081 tctcgcattt tctgcatgga agaatgaatt ggcagtctat caatttttcg gccaatttga
    10141 aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat cagatccaaa caggctttta
    10201 ttggaatagt gaagagaatc aaatcatttt tcatgttccg aatccagata aaataaattg
    10261 ggaagtatta gatattcctg atgagattac cttgaaacgc catgcttcta ttacttttgc
    10321 tgctagaacc gggaatgaaa gttcactaaa agcttatcaa ttttattggg aagaaaaaga
    10381 gcggacgatt acgtatcaat tccagatagg gagtggtcgc tatacaaaaa gaattgaata
    10441 aaacaggatt tgttttgttg gagtccctta cagcgcttat gatcgcttct gtctgtgtct
    10501 acttagtgag ctttggccag ctgtttttgc ttcaggcaga aaagaaaagt ggggaggaac
    10561 tgctatttat tcgaatgatt tatgaagata tcaagacgca gcggatgtat cacaaaagac
    10621 cagagcgaag ggaagaaatg atcccatacg atatccaaat tgtcgataat aatggagtaa
    10681 tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag tattgaaaaa acaagtaatt
    10741 ccagcattta cgctgattga atgtctaatt ggactgatga tattgagcag tctattgctc
    10801 acattcaatc ttttgattca gcaaaccatc caagtaagtg aatttttgca gcgtaaagat
    10861 caaaaagagt ggctaatatt tttagcacaa ttagaagaag aactcaagag cagtcacaat
    10921 gtctctgtga aaaatcagca actgcactac acgatcgagg ataaacagta tatcatcgaa
    10981 agatatcagg caatgatacg taaaagaaga acttccggcg gacatcaacc tatgttaaca
    11041 agtatcactg agcttcaatt attggaaaaa gaacacacaa tttcttttta tgtacacttt
    11101 gaaaatggag aagatggata tgccatatgg acaaaaaacg atcagtagaa aatgttataa
    11161 gggctctatt ttattttctg tcttgctttt atttctttta tgtacgtttt tattgatggc
    11221 agcattagaa agctaccgtt tgtcaatgga tcttaatata cgaatcaaaa attattatat
    11281 agcaaaaata atgaagcaga tggctttatc ggaacagtct acgcttgccg aaagttcgga
    11341 aggagttttt tactacacaa ctggatctgt aacatatcag tgggtacaac agtccctatt
    11401 cttagaggtg gaagtctcac cgtttatctt tcggttcaca gaagaagtaa aaaatgatac
    11461 agatactagt acagaatgat ggaatgttcg tgaaaacgcc ctcttttctg ttataacact
    11521 gtttttaata aaaaaatgtt tgccgtttca caaataaaaa acagtgaaaa cgattgaaat
    11581 tttgtcaatt gttcgataga attaaacagg tgaaaaaatt gttataatat atctagaaat
    11641 gaggtgcacc atgttaccag aaaaaatgga acaagggttt aatcaaaacc tggaagctat
    11701 tcagctttta cagaatgcat tgggaacttc ctttttagaa gcctatgtcg aaaatgcaga
    11761 aaatttaata gatgattatc aagtacgtgt ggtggatgga gtgccgacaa aagagacaac
    11821 gcaacggatc acagctcttt atgaagagtt gaagaaactt tctttcgagc cagaagaatg
    11881 gcgcagactg tctcaattac ttttattgaa gggcagccag acagaacatt tgcaggcgaa
    11941 ccatcaactc actcctgata gtatcggttt tttatttgtt tttcttattg aacaactata
    12001 cacaaataaa aaagcaccgg ttaagattct ggatattgcg gcaggaatgg gcaacttgtt
    12061 attaacagtt cttttaaacc tatcgaatgc ggggtaccaa acagaaggaa ttggtgtgga
    12121 tattgatgat acattgttgg ctgtcgcagc ttctacaagt gatttgacac aagcaaatgt
    12181 tcaatacttt catcaagacg ggttgcagga attattaata gatcctgtcg attttgcaat
    12241 cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa gcaaaagagt ttttaacaag
    12301 taccgaagaa ggccatagtt atgcacatca tttattgcta gagcaatcaa tgaaatatgt
    12361 gaagccggat ggttttggtt tgtttttgat gccgagtgga tttttagaaa cggatcaaag
    12421 tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt caaggaatga ttcaattgcc
    12481 agatgaattg ttccgtaata aacaatcgca aaaaagtatt ttgatcttac agaaaaaagg
    12541 accacaagca caacaagtaa aagaagtctt gcttgtgaaa cttgcttcat taaaagaacc
    12601 agagaaggtg acggaatttt tcaacgagtt taaaaactgg aaatcatctg ctctgtaatc
    12661 attcaacgag aaggagagtc atatgtctaa aacaattgca atcaatgctg ggagttcaag
    12721 tttgaaatgg caattatatc aaatgccaga agaaaccgta gttgcgaaag ggatcgtcga
    12781 acgtatcggt ttacaagatt ctatttttac catcaagtac ggcgataatc aaaaattcga
    12841 acaaatcatg gatatcgaaa accacgaagt agcagtaaaa atgctgttag atcagttgat
    12901 tgaactgcaa attttaagct cttatgatga aatcaccgga gtagggcacc gtgttgttca
    12961 cggaggagaa acttatgggg attcagtagt gatcgatgag aaagtaatgg accgcattgc
    13021 tgaattagca gaatttgctc ctttgcataa tcctgcaaac ttaatgggga tcaaagcgtt
    13081 taaaaagatt ttacctgaca ttttgagtgt ggctgtattc gatacatctt tccactcaac
    13141 aatgccaaag caaaattatt tatatagctt accggtgtct tattatgaag atttcaaagt
    13201 aagaaaatat ggcttccatg gcacaagtca tcgttatgtg tcggaacgtg cggcaaaatt
    13261 attgaacaaa ccgatcgaag aactaaaaat catcacttgc cacttaggaa atggtgtatc
    13321 gatcacagca gtcgatggag gtaaatccat ggatacttct atgggattca caccattagc
    13381 tggggtgaca atgggtacac gttcaggtga tatcgatcca gctgttctcc catatttaat
    13441 ggaaaaatta gagattgata ttgatgaaat gatcaatgtt ttgaacaaga aatctggtct
    13501 gctaggccta acaggattgt ctagtgatat gcgtgatctt gaaaagaact atgaaaaaga
    13561 acatattcgt ttagcttatg acattttcgt ggaccgcatc cgaaaataca tcggcggtta
    13621 tgtgacaacg atgaatggtg ttgatgcaat tgtctttact gctggtatcg gcgagaatga
    13681 cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg tttggttgtg aaatcgatcc
    13741 agcgttgaat gaattgcgtg gtgaagaagt agatatctct acaaaagaat caaaagtaag
    13801 agtattagtg atcccaacag atgaggaact aatgattgcc cgtgatgtgg agcgtctgcg
    13861 cggctaagcc aaagcattcg ttaggataaa agatgaaacg catgcaagag ggaattcttg
    13921 catgtgtttt ttttgcggag aagaccgcaa aatggtaatt taacttcaat ttcccttctt
    13981 ttttacagga tcttcttagg gaattttgct tggaaaagtg gtcaaaaggg aaaagactgg
    14041 cattttattt attgattttc aggaaaatcc caatatttta ttttcattca tctttataag
    14101 ggacattaca tatgtaaaaa gaaaaaacat ttttagatca gcctcgtggg ctgtcgactt
    14161 tattcttcgc tttatgttta gctagaaaac cgatccgaaa cattcattga tcgaataata
    14221 aaaacttgat tttccacctg tactttgcct gaatcgtgaa agcacaggta tgaaaatcaa
    14281 gttttttgtt gcgaaaaaac gtcctagtca atcagttggc cgagcggctg gcggctgatc
    14341 tgctcctgat caataacgag tgcttcttgg aagtgttgct gcaaaaagta gcggtcatgg
    14401 ctgactgcta ggagcgttcc ttgatagtct tgcagcattt cttcaatttc ttctcgagca
    14461 tagctgtcga ggtgattggt tggttcatcc aaaatcagga cgttgatttt ttctaaaaag
    14521 agtttcagca acgccaaacg gattttttca ccaccggata aatctttgat ccgtttaccc
    14581 acatcgcttt gataaaagcc acttttggct aattgccgtc gagcgacttc ttcgtttcca
    14641 gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat cggcaaactg cagctgctgc
    14701 ggcaaataac cgatttgcaa gctactacct aatttgatgg tgccaccgta aggcttttcc
    14761 tcaccgagta ataatttgag taaggttgat tttcctgcac cattgtcgcc gatgatggct
    14821 aaatgatctc cacgatagac tgcaaaactg ctgtctttga atagtacttg atcagcgtac
    14881 tgctggtgca agttttgaat gatgaaaact tctttgccag attttccacc attagacaag
    14941 ctttgcagcc gatttttcgg gggaggtggc ggtttgacga gtgtcagttt cgccaggcgg
    15001 cgttccagtt ctttggcttt tttgaagaag gactcattgt ctccttcatg tgcccactga
    15061 cgaaaacgac gaatcatgac tttcaaccgc tggacttctt tttgctgcag ttcgtaattt
    15121 tttgtcagct cagccaagcg agcttctttc aagactcgaa aacggctgta gtttccggga
    15181 tactcaatca atgcaccttc ttccagttcc catgtttttg tggtgacttg atcgagaaat
    15241 tgccgatcat gagaaacgac cagaaccgat ttttttgtat tctgtaaata gttttccaac
    15301 cagcgaatac tgttaagatc taaatgattg gtcggttcat cgagtaaaag taaatcggca
    15361 tcggctgcta aaattttggc taaagcgact ttaactcgtt cgcctccgct aagttctgtt
    15421 aaccgtgctt cctgacggtg tggaatccct aagccttgca agatcgctgc aatccggtct
    15481 tctaatacgt aaccgcctag ttcttcaaag cgatgctgca gctcaccgta acgggccaga
    15541 cacttctcta agtttgtatc tggttctgtc atcaatgctt cgtagcgact catttgctgt
    15601 ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt ggttgacggt cgcttcggaa
    15661 actgtaagtt cttgttcgac cgtcccgatc tttaagcctt ttttgcgact gataacaccg
    15721 ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg ttgatttgcc agttccgttg
    15781 ataccgacta aggcaatttt ttcaccagct tcaatttgac aggataaatc ctcaaataat
    15841 ggattcccgc cgattgtttt tttaatagaa attgcttgta tgatcatgat ctctctcctt
    15901 gagaccactc aaaaaggcca tggttatcca tggcctgtgt tatttaaact cagattatca
    15961 tgagaaaaat accttgagat ggccattgat tttacgtgaa ttcaactgtt ttggacaaac
    16021 ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct aaatgcgata tggtatcgtt
    16081 ggtaattttc attacttgtt attttagtca tgcgcatcat agtattttcc ctccttcgtg
    16141 tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt caactagcgc tgacatttac
    16201 ttaatattta tcgatgtgta attctgtcag agagcttttt ttatctgact gagggtaatg
    16261 tctcgtggac cacatcattg tattctgagc taaaccagcg ttcaggcgaa atcatatggt
    16321 gtttgataaa aaactttgtc tacatgactt ccttttgcca tttgcttaat accgttgcat
    16381 taaacagctc ttctctttgg aaatctcacc aacatcgcgt tcaggagtat cgttgtggtt
    16441 gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat ttaccgaatg tagcacttgc
    16501 cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg gcgataaaat cggtatccgc
    16561 catcgatact tcatattttt tcgtatcaga ttcatcggaa acttccacat tgacgaacca
    16621 ataagctttg gcatgcttag gtcgttttaa accacaccag ggcgactaat cgccctggtg
    16681 ttcacttttt tttattttac ttgaactcat tgactgtttt ccctttattt taaacattat
    16741 aaagaccgat gagtgcttgc cgcgtctttt gtttttatca cggttttttg tgcaaaaaaa
    16801 cctgccgata gttggcaggt cgtctacgtg ttaagctttg gtgtggtctt tagctggcaa
    16861 ttggttttct aaatcagttc ggacaacaag cacatcgcaa gctgcgtttc taataacata
    16921 ttcagaaaca gaaccaataa ataaacgttc tacagcattc aatcctgtgg ctcctaacat
    16981 aatcaaatct acttgattat cttctgggat ttctctggca ataatttgtt ttggtgagcc
    17041 atattctacg acagaggtta cattattcaa gccagctttc ttcgcattgc tttcataatc
    17101 tgccaatgtt tgctttgcca tttcagtagc ttgttctgca agcattccat caaaggatga
    17161 aacggtttgg aaagaacgtg tgtcaatgac atgtgctaat agaagttctc cgttatttcg
    17221 gatagctaca ttgaccgcct ttttaaaagc taattcagct tcatcagaac catctacggc
    17281 aaccatgatt tttttgtatt gttgtaacat aagcattcac tcctcaagtc aatcgtattg
    17341 tactctttta ttgtagaaca ctttagggaa aaagagaatt atttactgct ttggttcagc
    17401 aatttttttt gcaaatcgga tactgaaggt ggcagaaaca ataattacga tcagtaaaaa
    17461 gaccaaggca ataaagcgtt gattgaaaaa agctgttaaa atacccatta cgcctaaaac
    17521 agcatatgca gctccgtaaa attgtaggaa tccttgattt ttatcatttt tgttgatcaa
    17581 tacaaaaaag atatcctgtt tattaaataa ataatagcta acgaataata atactaaagt
    17641 ggcgataatc attaaaatcc taatcatgaa caaatcctcc aaaataagca acaaatagaa
    17701 aaagcaattt gccaagagca aattgcttta ttctttaaaa gatggtaatc tccgttaatg
    17761 ccgttgtttg tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc catggtcgta
    17821 gcttcgaact accaaatgat aaggcatgtt accagcagtg acacaagacg ggtcccaaga
    17881 tatcaataaa aatgttcggt caacacacaa agaggacaat tcgaacatca cagatttccc
    17941 acctctatag tagcatatga atttaaaaag agcaatgaga gagctcgcag aaacagaacc
    18001 gttttcttgt ctagtttttt ggaggatgct ttttccactc ttttatacgg taatactgtt
    18061 gacccagagc ctgttcatat ttccctgtat cttttggctg ataatactga gcattcttca
    18121 gtttatctgg taaatattgc tgattgaccc aagcctgatc aaaatgatga ggatactgat
    18181 aacccacgcc acggttgagt gatttcgcac ctttgtaatg gctatctctc aaatgatcag
    18241 gaacatctcc agctttgccc tctcgaatat cagcaagtgc tgcatctaat gccatgtagg
    18301 cagaattgga ttttggcgac aagcaaagat cgacgacgac gtctgctagt gggatacgtg
    18361 cctcaggcaa tcccaatttt tcagcagcta atacagcgtt cactgtacga gccgcagcag
    18421 caggattacc taaacctata tcttcataac cgataaccat cagacgccgg caaatacttg
    18481 ccagatcacc tgcctcaact aaacgggcga gataatgaag tgcagcatcc acatcacttc
    18541 cacgaatcga tttttgaaaa gccgaaataa cgtcataatg agcatctcca tttttgtcat
    18601 gggtaagagc ttttctttgg acacattctt caatgatcga cagtgtaaga tgaatccttc
    18661 cttctttatc tgaaggagtc gatagtgtag ccagttccag cccattcaat gcactccgca
    18721 aatcgccgtt cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac tggattgctt
    18781 gttgtccaag gccgcgttct ttatccctta aagcatgttc gactgccaac tggatgtctt
    18841 gttctgttaa tggtttgact tcaaagatct gtgtacggct gcggatcgct ggattgatgg
    18901 taatataagg attctcagta gtcgctccga tcaagatgat tcggccactt tctaaatgag
    18961 gaagcagaaa atcctgtttt gttttatcta atcggtgtac ttcatccaat aagaggatca
    19021 ctgtaccact catttttgct tcttccgcta caacttgtag atcctttttt gaatctgtag
    19081 cggcgtttag catgcgaaat gcataattag tagagccggc gatcgcactt gcgatactcg
    19141 tttttccagt accaggaggt ccatatagaa tcatggaaga gagcatgcga gcatcgacca
    19201 tgcggcgtat gatttttcct tccccgacta gatgctgctg accaacgact tcatcgatcg
    19261 ttcgaggtct catgcgataa gccaatggct gttgcatagg attttcctcc ttcttgtttt
    19321 tacgtccctt aattatatca tatttagcaa gatgcctaga aagaacagcc ttctgttgtt
    19381 ttgttttcat agcttatcca actaatattg tgtatgataa agtaggtgag aaaaatgacg
    19441 gataaacaaa agatagatga atttttttgg aataaaacga cgccagaagt ggcacgggat
    19501 ttattgggga tgtatttgga gcatgacaca ccagaaggaa gattagctgg atatattgta
    19561 gacgcggaag catacctagg tccagaagac gaggccgccc atagctatgg tttaagaaga
    19621 acaccgcgtg tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta cacgatgcat
    19681 actcatcgga ttttgaacat cattactcag ccagaaggga ttcctcaagg agtaatgata
    19741 cgtgcaatcg aacccgccgc aatgattgat caaatgagta aaaatcgtgg cggaaaaact
    19801 ggtccagata tcagcaacgg tccgggaaaa ttagtggaag cattagcaat tcctcaagaa
    19861 ctttatggtc agtctatagc agatagttcg ttacgcttag tttttgagaa aaaaaagaca
    19921 ccaaaaaaga tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt atggacagaa
    19981 aagccgttgc gttttgttgt gtcgggaaat ccttatatct ctttacaaag aaaaaaccaa
    20041 atcgagaaag attggggttg gaggaaagaa aatgaaaaag aaggatctat taacatattt
    20101 agatgaaata attgaaaaac aagtgacaga ctatgatgta gctttagatt gggatacaaa
    20161 aaatcatacg atcgagatcg ttgttcgctt gtttgcagaa aataaagcac aatttgagat
    20221 tgatgatgca gaagggatcg tttcgcaaga accaattatc gaatttgaag acggcgtact
    20281 tctttttaat cctcagaaat ctgtttttga tgaacgcaat tatctagcag taatccctta
    20341 tgaaggaaaa aaagggttgg caaaatcagt agctgattct ttagtggaat atctaaatga
    20401 agtattagct caagggcaaa gcgatttgct tgattttttg gatgaagacg atgacgaagc
    20461 tgtattcgag cttcactggg ataatcagca actagcagaa ttagtagaag taaaacagca
    20521 aaatgaagca gatacttatt taccgtaccc aagttattaa ggagtgacag atatgaaatg
    20581 gaatgaagta aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta atattttgat
    20641 ggaggcaggg gcgagtggtg tcgcgattga agattcttta gatgttgaac attttcagtc
    20701 ggatcaatat ggtgagattt tagataaaaa tgattttaca tcgctgaaag aaggcgcact
    20761 agtcatggct tattttccgg aaacgacttt cttaccggaa atcttgcctt ttatcaaaga
    20821 tcgtatcaca caattacctg agtttggtct agctattgga aaaaataaag taacagttag
    20881 tgaagtagag gaaagcaact gggcaacagc ctggaagaaa tactatcatc cggtgcgtat
    20941 tacgcgaatg ctgacggttg cgccaagctg ggaaaactac cagacagaag atcctctaga
    21001 aaaaattatc acactagatc ctggtatggc atttggcaca ggcactcatc ccacgacttc
    21061 cttgacattg acagcactag aaacagtctt gcgtggtggt gaaacattgc tagatgtggg
    21121 aactgggtcg ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag atgtctatgc
    21181 ctttgattta gacgaagtag ccgttcgttc ggcgaaagaa aatatggata tgaatgaaat
    21241 agccaaagat gtccatgtat cagctaacga tcttttgaca ggaatcgaga ttgaaagtga
    21301 tgtaatcgtt gcgaacattt tagcagatat catcttattg atgatcccag atgcttggcg
    21361 tttactgaag caaacaggca cgctgatcgt ttcaggaatc attgaagaga agaagcaaat
    21421 ggttctggat gccatgttag aacaagggtt tatcgtggat caaattttcc agcaaaaaga
    21481 ctggtatgca attattttga aaaaaccaga ggtggaatag atgcagcggt attttttaaa
    21541 agaatcatat gaatcaaaag actattttgt tttagaaggg gaaaactatc accacattgt
    21601 acgggttatg aggatggaac ccgaggatga agtttttttg gcattcaatg atcaaatagc
    21661 cattgttgca gaaatcacgg agattactga agaggctgtt tatctgaaag aaattaaaaa
    21721 agaagaaaat caaaaggaac tgccaagttc gattaccata gcgagtggct atcctaaagg
    21781 ggaaaagctg gattggatcg ttcagaaatc cactgaatta ggagcgcaag cttttcttgg
    21841 tttcccggca tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga aacgacaaca
    21901 acggcttgaa aagattgctc aagaggcagc ggaacaatcc catcggcaga tcgtgccatc
    21961 agtagagtta ttggaaacga aacaagattt atttgagcgg ctagatgatt atgacgtggt
    22021 cttggttgct tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag ccaaaattct
    22081 tagtgaaaca aaaccagata gtcgaatctt agctgtcttt ggaccagaag gtggattatc
    22141 ccgcgatgaa gtcgattatt ttgtagaaca caaagggata ctttgtggat taggaccaag
    22201 aatccttcgt acagaaacag cacctcttta tttattaagt gctgctagct atcaatggga
    22261 attaatgaat ccttctttca gctaacgttc ttcattaata aaagagtatc gacagaaaga
    22321 attgaaaaaa tagtcaacaa tttgtataat agaaacaatt atttatacaa tggatacgaa
    22381 aaatcgtttg aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt ataactgcac
    22441 ggattttggg acaattatca aacagatatt tgtcccgatt cccgttttac cgaaaaaaga
    22501 aagggggatc gattcctgtg acaaaagaag aaattttaac gggacccggt gtcataaagc
    22561 tcgttagtca atacatgggt cccgaacatg tagcatttgt tgagaaagca tgcgaatacg
    22621 cgactgctgc acatgacgga cagtttagaa aatcaggcga accctacatt attcatccta
    22681 tccaagtcgc aggtatatta gcagatttaa aaatggatcc ccatacagtg gctacaggct
    22741 tcttacatga tgttgttgaa gatacagaaa tcactttaga agatctgaga gaagaatttg
    22801 gcgatgacgt tgctatgtta gtggacggcg taaccaaatt agggaaaata aaatataaat
    22861 cccacgaaga gcagctagca gaaaaccacc gaaagatgct gctagcaatg gctcaagatt
    22921 tacgagttat catggtcaaa ttagctgata gactacataa catgcgtacg ttgaagcacc
    22981 tgcgagaaga taagcaaaga aggatcgctc aggaaacttt agaaatctat gcgcctcttg
    23041 cacatcgtct agggatcagc cggatcaaat gggaattaga agatacagca cttcgttatc
    23101 taaatccaaa acagtattac cgtatcgtcc acttgatgca gacgaagaga gaagaacgtg
    23161 aaaaatacgt aagtggtact gttgaagata ttcgaatagc gacagaagag ctggggattt
    23221 ttgcagaaat ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa atgaaagatc
    23281 agaaaaagca gttcaacgaa atttatgact tgctagctat tcgagtgatc gtagattcaa
    23341 tcaaggattg ttatgctgta ttaggagcaa tccacacaaa atggaaacca atgcccggca
    23401 gatttaagga ttatattgcc atgccaaaag ctaatatgta ccaatctttg catactaccg
    23461 tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa atgcatgaaa
    23521 tcgctgaatt cggggttgct gcacactggg cctataaaga aggaaaaaat gaaaaagtag
    23581 aaccagatgg tatgacgaaa caattaagct ggttccatga gatactcgaa cttcaagacg
    23641 aaagctatga tgcttctgaa tttatggaag gcgtaaaagg agatatcttt agtgataaag
    23701 tctacgtctt cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc ggaccattgg
    23761 actttgcata cagtatccat acagatatcg gtaacaaaac cactggtgca aaagtaaatg
    23821 gcaaaatggt gcagcttgat tacaaattga aaaacggaga tatcattgag atcatgactt
    23881 ctccaaattc atttggccca agtcgcgact ggttgaaatt agttgctact agcaaagcaa
    23941 gaaataagat caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg atcaaaggcc
    24001 acgaatccgt ggtcaaatgt attacagatc taggatttac gcctaaagat attttgacga
    24061 agaacaaact gcaagaagca ctcgatcgtt ttaattatca aacagaagat gatctctatg
    24121 cagctgtagg gtatggagaa gttagcccct tgacgatggc caatcgtctg actgaaaaag
    24181 aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc atgaatcagc
    24241 cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc attcaagggg
    24301 tagaaaactt actaattcgt atcagtcgct gctgtaatcc gattcctggt gatgatatcg
    24361 ttggttatat cactaaaggc agagggatat ccattcatcg tcgagattgt ccgaatgttc
    24421 agcctgacaa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa gatacatcga
    24481 atacacgaaa agagtatgat gcagatttgg aaatttacgg ctataatcgt tcaggcttat
    24541 tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc gttgaagcac
    24601 ggacgaataa agataaaatg gcgacgatcc atttgacggt tggtatccag aatttatccc
    24661 acctaaagag tatcgtggat aaaatcaaag cagtacctga tgtctacagt gtacgccgga
    24721 cgaatggata ggaggacaga tatgagagca gttgttcaac gggtaagtaa agctgctgtg
    24781 tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc tacttgtttt attaggcatt
    24841 catgaaaccg atacgcaaaa agatgtggac tatctggtaa aaaaaattgc acaaatgcgt
    24901 atttttgaag atgaacaagg aaaaatgaat attagtgtag aagatgtggg aggacaaatt
    24961 ttaagtatct ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg accaagcttt
    25021 gtggaagccg cacgaccaga aacagctatt cctttatacg aatcattcaa tgaaggaatc
    25081 agaagtcgcg gcatcacagt cgctaccgga caattcggag cagatatgtc ggtttctctc
    25141 gtcaatgatg gacctgtaac gatcattatt gattctaaaa acaaataaag taatctggtt
    25201 ttcactagta aaaactatga aacacgtcgc ttaagttaga ctgataaact ctaacttaag
    25261 tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa agttaagctt
    25321 aaattataca agttattttt tataggaggt tgctgaagat gaaagaagta ccagtagtag
    25381 atatcaaaat cacagattta aaaaaattgt atcaaaaagg atacaacatg ttggaggagt
    25441 tacgccatga agcggacgct cccgtcgtca aagcaaagat tttcaataaa gaagctatca
    25501 cgatctacgg atcatcagca gcaaaagtat tttatgatcc aaggaatttc aaacgaaaag
    25561 gtgctatgcc aaaattagta ttgaagacac tttttggtca aggtggagta cagactttgg
    25621 atggagctgc tcatcaccat cggaaaaata tttttatgga tctaatgaca ccggaaagaa
    25681 tggaagatta tcatcgtatc ttagataaga atctgacaca agcacttgaa gcacagcatg
    25741 gtcaatttga actatttgat ttaagtaaga tggttttctt tacatctatt tgcgagtggg
    25801 caggtatcaa tttatcagcg atttctaaag atgaggtcga aaaattagca gaatatcaaa
    25861 tctctatgat cagcggaacg tttacttctc cgattgatca cataaaaggt gtcgaaaatc
    25921 gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa aatcctgtgg
    25981 ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc cacagattta gatggtcagc
    26041 ttttacctct ggaagtggca gctgttgaat tattgaacat catccgtcca acggttgctc
    26101 taactgtgtg ggcggcattg atgggacatg cacttttcag tagaccagat ttgtatcagc
    26161 aattaaaaaa tgacttttcg acgttacaag atccgtttat tcaagaaatg agacgctatt
    26221 atcctttctt cccaatgcta cctgcgattt cgctgaaaga agtagaagta gatggttatc
    26281 gtataccaga aggcagttgg gttattttag atttatatgg aacagaccat gatgaacgaa
    26341 cagttgaagc gccagattcc tttatgatca aacggtatgt agggaaagca aaagatattt
    26401 cctataaaga agaatatgag atgattgcac aaggcggggg aaattttcga caaatgcatc
    26461 gctgtgcagg agaatggatc actcttcata gtctacgtgt gttttccgat cagctagtaa
    26521 ataagtttga gttctctgta cctgaacaag attggacgat tccattcaat cagtttccga
    26581 cttatccgaa tagccgagcg ctactttaca agaattagat tagcataaac ttatcgaaaa
    26641 aaaggaagat caaacagtgc tgatcttcct tttttcatat tactataaat tgataaggtc
    26701 aattgatcaa aatccacgaa atgcttcatc aatggtttga taatcttcat tggataaatg
    26761 caaatctaaa gcttggacat tgtcagatac ttgctctgga cgcttagctc caggtataac
    26821 tacagacact cgtggattct tcatgtacca agctaaaaca agttgcgcag gagtggcttg
    26881 gtatctttta gcaatggggc gaagtacatc gacagcggtt aaaatttctc tgaaacgttc
    26941 tccttggaaa tctggatttc tttttcgagg atctccttca ccaaattgtt tttcctcacc
    27001 aagttcatat tttcctgtca gcaaaccgga agcaagagga aaatagggaa caaaactgat
    27061 tttgtttttt tcaagataag ggaataattc tttttcagcc tgccgatgga tcaaactgta
    27121 tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt gttctaacgt
    27181 gaagtttgac acaccgactg ctcggatttt tcctgcttct tttaattcgt gcaatgtagc
    27241 aactgattca ttttttggtg tgctttcatc cggaaagtgg atataaaaaa tgtcgatgta
    27301 atcagtttgc agacgtttta gtgcatcttc caccgattgt ttgagaaact caggagaatt
    27361 atcaaatgtt cgccctttat ttggtacatg agcagctttt gtagcgatga cgacacgtga
    27421 gcgatcatat tctttttcct tcagtacctc gccaatcagt tcttcggaac ggccgtttcc
    27481 gtaagcataa gcggtatcga tcaaattgat cccgctattc aaagctgtac gaactacttg
    27541 ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt cctccaacgg catttgctcc
    27601 taatccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg ccatagctga
    27661 ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa acgtttttgt
    27721 gaggggaaat agtcagatat ctcttgactt tgagactggt ttttagtagg ttataagtaa
    27781 tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact ttacaagaga ggatcatcga
    27841 ggctgaaaat gatctaaagt ttcattagcc taaagacact ctggagatgg acgagtgaaa
    27901 agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag aaatctttca aaattaggtg
    27961 gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa caagggtgat ttttttattt
    28021 acatagctaa gaaagggaga gaaaactatg agtttccaaa gaccaaaagg aacaaatgat
    28081 attttgccag gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg tttattattc
    28141 agagattatc agtataatga aatgcgtaca ccgatttttg aacactatga agtgatatcc
    28201 cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga tgtatgattt ttatgacaaa
    28261 ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac caatcgttcg ttcttttgtt
    28321 gagcataaat tgttcggccc agagtatgcg aaaccgttca aaacttttta tatgggacca
    28381 atgtttcgct acgaacgccc tcaaaaagga cggttacgtc aattccatca aatcggagta
    28441 gaagcatttg gatcagaaaa ccctgcaact gatgtggaga gcatggtgat ggcacttgat
    28501 ttcttcaaac agttggggat cacgcaaatc cggctagtca tcaattcatt aggtgataaa
    28561 gaaacgcgtc aagcctatcg tcaagcattg atcgactatc ttttaccttt tgaatcagag
    28621 ctgagtgaag actctaaacg ccgccttcat gaaaatccat tacgcgtatt ggacagtaaa
    28681 gacaaacgag atcaaaaatt tgtggcagaa gctccatcta tcttagatta tctaagcgag
    28741 cctgctcaaa aacattttga taccgttgtt tccatgctgg atgcattggc tgttccttat
    28801 gaaatcgaca gtaatatggt tcgcggacta gattattaca cacatacgat ttttgaaatc
    28861 atgagtgacg caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg acgttatgac
    28921 aatctagtgg aagaactagg tggtccttca acacctggct tcggatttgc aatgggtatc
    28981 gagcgattac tcttgactat ggaggcagag gaagtagtta tccctgcatt taatgagttg
    29041 gatgcttacg tggtcgcact cggagacgaa acaaatatcg aagcattgaa agtggtccaa
    29101 gcgattcgaa actttggttt ttcagctgac cgcgacttca tgaaccgtaa agcaaaagcg
    29161 caatttaaga cagcagataa agcgaatgcc aaattagtat tggtgatcgg tgaagatgag
    29221 cttgctagtc aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa aagttttcca
    29281 ttagaagaga tatatgaaca ttttgatgaa gtctatgatg aaatgacttt attcgagtag
    29341 gaggaaaaaa ataaatggca caaagaacga tctattgcgg attagtttcc gaacaacaat
    29401 tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt gggggagtaa
    29461 tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat cctgaaatca
    29521 acaaagaagc atgggaaatc gcggataaat gtcgcagtga atacgtgatc gaagtgactg
    29581 gggaagttag aagacgcgga gaaaatgcag taaatcctaa aatggccact ggtgaattcg
    29641 aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aaccccgcca tttcaaattg
    29701 aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg gatctgcgtc
    29761 gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg attcgtcatt
    29821 atttagatga taatgacttt ctcgatatcg aaacacctta cctagggaaa tctacaccag
    29881 aaggcgcaag agactaccta gttccttcac gtgtccatgc tggacatttc tatgcgttgc
    29941 cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc tggattcgac cgttattatc
    30001 aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct gaatttacgc
    30061 aagtagatat tgaaacaacg ttcttaacag cagaagaaat ccaaacttac acagagggat
    30121 tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca tttcctcgta
    30181 tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca cgttttgata
    30241 tggaattgat cgatctaagc gatacagtca aagaagtaga attcaaagtc ttccaaatgg
    30301 ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca gatcgctact
    30361 cacgaaaaga catggatcag ttaggtcaat atgtaggaca attcggagct aaggggcttg
    30421 catggctgaa agtagaagaa gacggattga aaggtccaat tgctaaattc atgggagaag
    30481 caacagaagc aatcattaaa gcaacagatg caaaaccagg tgacttgctg atgtttggcg
    30541 cagataaaag tgaaatcgtc gctgctgcat taggtgctat ccggacacgt ttaggaaaag
    30601 aattaggact aattgatgaa tcgaaattca atttcttatg ggtaactgat tggccacaat
    30661 ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc acgatgccaa
    30721 aagaagaaga tatcccgtta ttggctgaag accctgcaaa agtctatgca gaagcttatg
    30781 atatcgtctt gaacggttat gaattaggcg gaggttctat ccgtattcat actcgtgagc
    30841 ttcaagaaaa agtactagaa actctaggct ttacaaaaga acaaatggaa gaacaatttg
    30901 gtttcttgtt aacagcttta gattacggat tccctccaca tggcggtatt gcattaggat
    30961 tggaccgttt tgcaatgtta ttagccggag aagataatat tcgtgaagtc attgcctttc
    31021 ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc aagtatggtt tcgcctttac
    31081 aattgttcga attgaatatt gatgtaacga atatagaaga atagccgata aaaaggtaaa
    31141 aaggatttgt cgaatacgct tcttttgaca accttgattt aattgaaaaa cactttataa
    31201 tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa aattgggctt
    31261 atcatgatta cacgaccaag ctttcggtat ctatcgttta tgcgttgatc gcatcagtcg
    31321 cattgaattt cttttatcag ccaggacata tttattcaag tggtattact ggtctagctc
    31381 agattttgac gactttgtca aaatcactga taggattcga agtaccggta tcaacaactt
    31441 tgtatttact gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg aagaagttta
    31501 caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt ttaccggaaa
    31561 cagtcctatc taccgatcca atcatctgtg cgttgtttgg gggggctgtg atgggactag
    31621 gtattgggct agcgttgaga aacggcctat catcgggcgg tctagatatc gtgagtatca
    31681 cgattcgtaa aaaaacagga aagtcgattg gttctatttc gatctacttc aatgcattga
    31741 tcgtttttgt tgccggaatt ctatttggct ggcaatatat gttttacagt acgttgtcta
    31801 ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaag atgcaggtaa
    31861 tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt atgcgtagag
    31921 ggatcacgat catcaatgaa gcagaaggtg cttataagca tgataagcag acagtattga
    31981 tgacgatcgt tacacgatac gaactgcctc tacttagaga agcaatgaaa gcttctgatc
    32041 cacaagcttt tgtaagtatt gcagaaaacg tccagatact tggtcgtttc tatgaggatg
    32101 gcatttagat cgagtttgac aaaaattcat gaaatactgc tgtagacaga cgttcgtttg
    32161 tctgcagttt ttttctacca tagtagaatc agcaaagaaa aactgctttt ttatttgaaa
    32221 ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa tttatgggat gatagaaaaa
    32281 aatagtagag aaaggacacg attctacttg ctattttcac ttaatacccg tatattacga
    32341 atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag gttaaaaaac
    32401 agccgctaca tcactgggtt tgatgggctc cgttcattag ctgtggtggg tgttatcttc
    32461 tatcaccttc tgccaactag tatgaaaggc ggctatttgg gcgttccgat ttttttcgtc
    32521 gtatctggtt atttgatcac ggatctgctg cggcaggaat gggaacaaaa tggaaaaatc
    32581 aatatttggc aattttatgt cagaagaatg aagagactct atccggcact tgtttttatg
    32641 cttgtgacag cttcggctta tatcactttg tttcagcgag ggctgttaaa caatcttcga
    32701 ggagttgtgg tcagcagctt actgtatgtc aacaactggt ggcaaatcaa taacggttta
    32761 tcttatttcg atcgatttgc taatgagtca ccgtttacgc atatctggtc tttagcagta
    32821 gaaggtcaaa actatttggt ttggccgatc ttgtttgtat tgttgatggt atttgttcgt
    32881 aaaagaaaat ggatctttta tactgtgtta ggtacatcct tcctctccgc catcttgatg
    32941 atgatcttgt atacaccagg aggcgatcca acccgtgttt attacggtac cgacactcgt
    33001 ctgttttcta tatggttggg aagtgcgctt gcgtttgttt ggccaagtac gcgtttaaag
    33061 aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat ctcgcttttg
    33121 gcactgattc tttgcttctt ctttttagat gatcattaca atttcatcta ttacggagga
    33181 atgctcctag tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca ccccggagca
    33241 agtttgaatc gatggctgac aaatccagta tttacttgga ttggcaaacg aagctatggc
    33301 atttatctgt atcagtttcc agttatgatt ttttatgaag caaaggtgac tgatattgcc
    33361 gatcatgttt tgttgcatac tttagtggaa atcacattga ttctaatcat cagtgaactt
    33421 tcttatcgat atgtggagcg tccgctagca cgttttgatt atggtcgtac attccaagtg
    33481 atgaaaggat ggttcagcaa accgatcatc agtaagagca aaccatgggt tgttccaggc
    33541 acattacttg tagcaattgc actcgttggt ttcgttacgg caccgaaaaa cgctgtaaca
    33601 gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta aaaaattagc agaagaaacc
    33661 agaaacaatt cagaaaacac cactagttcg gttgaccaag cgatccttga taagtacgag
    33721 ttgtccaagg cacaagggaa taaggcacaa aaattagaac taacggcatt tggtgattca
    33781 gttatgttgg atgctgcaac ggatctacaa gaactttatc caaaagtagt ggtagatgga
    33841 gatgtcggac gacaattata tgccagtgta ccgtacattg aaaaattaaa ggaacaagat
    33901 ttgttgaaag acactgttct gattggacta ggaacgaatg gctcattctc ggaagctcag
    33961 tttgatagtg tgatggatgc aatcggtgat cgaaaagtat actgggtcaa tgtccgagta
    34021 ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac gaaaaaatac
    34081 gacaatatga cgctgattga ttggtacagc tacagtaatg accatagtga ctggttttat
    34141 gatgatcaag tccatccaaa tcctgaaggg atgacacatt acattcactt agtcagcgaa
    34201 gccttgctag gaggaagttc aagtacgaac agcagtgaga caaacgtgac agaatgatag
    34261 ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga agttttttta tatttaccgt
    34321 gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg taaataacgt
    34381 tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc ttcgcttctt
    34441 gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag acgagtatat
    34501 ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt tgtatatcca
    34561 ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt tgccatagag
    34621 catctcacga atctctgcag tgtattctcc atccatattg taagtgatca atggtggagc
    34681 gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa tatttgcctc
    34741 ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat agtcttccat
    34801 gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc gtccatttgt
    34861 ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa tctcatgacg
    34921 ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt tgaaataagg
    34981 tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc tgtctttcag
    35041 atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac tacgtcttgc
    35101 catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg ttcgtttact
    35161 taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc cttttttagg
    35221 tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca ctttggaact
    35281 ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt cgtgaagcat
    35341 ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta ttaaaaagaa
    35401 ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag aagggaaagg
    35461 aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta gccttagtta
    35521 atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat tacatactag
    35581 ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca gcacccaagg
    35641 aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga tttatattaa
    35701 ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc attaaaacac
    35761 ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt gggacaagac
    35821 atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa gcgaaaatcg
    35881 tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt cagagagtga
    35941 cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat aaagaaggta
    36001 tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa gaagtgaacc
    36061 ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt gggcggtctt
    36121 ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga gagacgtcaa
    36181 gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat gtaatggatc
    36241 aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt ttttacatac
    36301 agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag accagcgtca
    36361 tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa tcgtgattgc
    36421 cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat acaatgaatt
    36481 gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag gaaatcacga
    36541 tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact attttttgcg
    36601 aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat ttttctttct
    36661 tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata tccgaacgat
    36721 caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt cagaaaaaaa
    36781 acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt cggaaacgaa
    36841 aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt ttgattatgt
    36901 agcactagga catttgcata atcaagctgc tttgagacag gataatgctc gctatagcgg
    36961 ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat ggatcgttga
    37021 cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg atattgaaga
    37081 aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg tcaatcggga
    37141 gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga tgaaccagct
    37201 tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag cagaacaaac
    37261 aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag aagcattttt
    37321 tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg gagaaacgtt
    37381 gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac aacttaaaaa
    37441 ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag caccgctttt
    37501 tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga tcaccttcgc
    37561 actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt ctttgtttgc
    37621 tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga tgagctatga
    37681 aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa caaaaaaaca
    37741 ggcagccaaa gtaacgttga ccatttttga tgggaaagga aaagaaataa aacaaatcac
    37801 aaaacgtaca gaagcagatc agttgatcag agaactgatg aatctcgatg cgaaacagtt
    37861 ttctcaaatc gttttactac cccaaggaga attccgtaat tttttggttt catcaagcag
    37921 tgataaagag actgttttac gtcatttatt cggtactcaa tttttccaac agttcaatga
    37981 gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg gatcatttag agcaagagct
    38041 gcatcttctg caaaaaaggt ttgttttagt caaagaagag gaaccagcag cagggtttga
    38101 agccgtttta gcccaatgga caaaccatca ggcaatactc gaaaaacaaa tcacggaaga
    38161 aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt cctactatcg
    38221 ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca agcaaaagga
    38281 attagctgct caaaaagacg aaatagagga aaagaaacga tggattgggt attatgaatt
    38341 tacgcaacga ttagtcgaac caatccagcg cctacaagaa ataaaagttg aaaaaataga
    38401 actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg atgactacgc agaattatga
    38461 agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca aagaagctgc
    38521 tcaaaaactg caaaacaaac agatgatggt gccaattgtc gaatccttac gtagtaaaaa
    38581 acaagacgca gaacaattaa caatttcatt ggataaagaa gaattgaatg ccgagaagct
    38641 gtatcaaaat ctcgctatct atcaggaaag aactcgaatt cttcaagaaa atttactgga
    38701 tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc tagatgaaaa
    38761 gcagcaagaa atagaaaaac aaaaagcaaa agacactggg tcgctcgcac agataagaga
    38821 agaactcaaa aaagcaactg aagaaataac aaaaaatagt cagctattga aagaacagac
    38881 agaacaagca gaacagctga aaagccaatg ggcaaagcag caaattgctc gtcttcagct
    38941 ttttttgcgt ccaggggagc catgtcttgt ttgtggatca acggaacacc ccatacaaca
    39001 attaaaacac gaacagccaa ataaggaaga tatcttaaaa gtagaacaac aactggcaga
    39061 agctgagtct gctgtagaaa gaactcagca aatcattgcc aaaagtgaag cacatgaaaa
    39121 taatcttcaa caacaattat cagatttaga acaaaaaatc atggcaacga atatacaatt
    39181 ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag gcatttcctg agataacaga
    39241 acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct ttagatagac gaaaaagcaa
    39301 gatggccaat gcaagaatag agctggaaga aataagacaa caagcagaaa agctcgaaca
    39361 acaattagct gtctacaaac agcagcagac agaaaaaaat aatcagctcc agcaactgca
    39421 aggagaaatc aaaagtttag aacaacaatt gcaagatacg gatagtaact gtttagaaga
    39481 ggagatacga cgacttgaaa aacagttaga agaagactcg aatcaactag ctgttgagca
    39541 gaaagcagga gtgcaattgt tgcaagatct tcatacgctt cggacacagc ttgatcttca
    39601 acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa gaatagctaa
    39661 aaagttagaa agccagtcgt atttttcaac aattgaagaa atctgtgcat ttgcacaaca
    39721 gcaagcacaa tataaagaaa accaacgtac agttacttct tatcatgaag agcaattagt
    39781 gacagctgac cggctgcgtc aattagaaaa tatccagcta actgagacat tcccagatac
    39841 agaagagatc agccaacagt tagaagaaat aagacgtatg atcgaaataa agcaagaaaa
    39901 gatatacggt ttacaagaac aaaaaattag taatcaaaaa atctacgatg aatgtctttc
    39961 gatttatcac acaagccaga cgaaattaga tgagcttacg caattacagc agctttcgca
    40021 aacattcaac ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc ttcaggttta
    40081 tcttcaagaa gtgttacaag tagcaaatga tcatctccaa cgtttaacga aaaatcgtta
    40141 tcaatttgag ctagcggata ccattggcag ttatcgaggg aaaaccggat tagaaatcaa
    40201 tatctatgat gatcaggcag gtatgtctcg aagtgcgcat accctgtctg gcggtgagag
    40261 ttttattgca gcactttctt tagcattagc tcttgccgat gtgatccaga cacaagctgg
    40321 tggtgtagca atcgatgcat tgtttattga tgaaggattt gggtcattag atgaagaagc
    40381 tttggaaatg gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa tgatcggcat
    40441 cattagtcat gtaagagaat tgaaagaacg agtattacaa caaataagaa ttgatacaga
    40501 aggtagcgga caaagcaaaa tacgctatca acttggataa aaagggagat tggtaactat
    40561 gaaatggagt attccagaaa aagtcattgc acgtggacgt caatacttgc aagaaggccg
    40621 agtgttatcg gttgtccctg accaagaaga aaacgtgtgg catgcagaag tcttgggcag
    40681 tgaattgtat atggttgatt tggatggtac agcaaaagag atagattact gccaatgtcc
    40741 ctattgggag gaacataaat attgtaagca tacagttgct gtggaacttt atttacggtc
    40801 gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg taaaagcaac
    40861 aagtgaggga gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa acaatcctca
    40921 agcggtgcaa cctttgatta ttgaatacca agtagaaacg attgagacta atcagtatca
    40981 cccagagctt tctattttgg cactttactt gcgtatcggt tcattggcat caccgaaaaa
    41041 aacatatatc gtaaaaaaca tctatgattt tttacaagct tacacagaaa aagaaactta
    41101 cactgttaat aaacagtacc agtttcgtct agataaacgg gcgtttcaaa aagaagatca
    41161 aaaaatactc acgcatcttg caggaagtgc tcagacgcat caattattag gtacgaacgg
    41221 cttacaagtc aaaggaaaac tagataaaag atatctgctt ctcccagtag aacaagcccg
    41281 cttcctgtta gagcagatga atcagactac acgtttgcat ctgcagataa atgaacaaaa
    41341 cgatcaagga atacaatttt ccgatcaaaa caaaccttta tcattcaaag tggaaaaatt
    41401 tggagaagat tatttgctgc aagcattgaa tgattttgat tttttcttta tgcattatca
    41461 gtggggagtt atccaaggga cattttattc tctgacgaaa tatcaacaaa cggtttatca
    41521 aacttggaaa caattgattc gccgattgga gaaaccagaa gtagtgttca agaaaaaaga
    41581 attaccgtct ttgtttaaag agattatccc tctgctgtca gagatcggcg acgtagctat
    41641 tgcccctgca gtagaagagg aagtagcaga tatccccgtc gaatttattt tctttttccg
    41701 aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc tatggtgaag tcatctattc
    41761 gactgacccg aagcatgagg tctcgacaga acaggctttt caggtgttac gtgacacagt
    41821 agaagagaaa cgagtacttg atctattcag aatgtatcgc taccagaaaa atgaaactgg
    41881 ttatgaacgt ccattgccaa gcggggaaga actatatgcc tttttccgca cagaactatc
    41941 tgtcttccgt cagtttggtg aagtcagact tggaagaaaa ttgcgagagt tgtatttaga
    42001 tgcgcataaa catcaaccga aaatcgaagt aaccgagacc ggttcatggc tcgatattcg
    42061 attcgatgtg acagggatag aggagcaaga aatcgatcat gtcctgcaaa gcttgctgag
    42121 aaatgatgct ttttatacat tagaaaatgg tcaggttctt tcttttgatt cagaagaatt
    42181 tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg atacgtacag aagaaggaac
    42241 gatccatgta cctaaaaatc aaggattgat cattcaaaat caattggaaa aaagcaatgc
    42301 tacttttagt gaatcgttcc aaacgatggt acaagatctc attcatccag aaagatacca
    42361 ggcacagctt cccaaaggat tgaatgcaac gatgagagat tatcaaaagc aaggttttcg
    42421 atggttgaag atgttaggtc attatcagtt tggcggtatt ttagccgatg aaatgggact
    42481 tgggaaaaca ctacagacaa ttgccttttt actttcggaa aaagaagaac ggaaaagctt
    42541 ctctgctttg atcgtagcgc ctgcgagctt aatttacaac tggcaggcag aggtgagaaa
    42601 attcgcccct tctttaagta tccaagtaat caatgggaat aaaaaagaac gtgaagaatt
    42661 actggctaaa gacacagata tccgagtgac ttcttatgcc agcttaagac aggatctagc
    42721 taattatcag tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa tggtcaaaaa
    42781 tagcagtacg aaaactgcac aagcattgag agaattagct gtacctcagc ggtttgcact
    42841 gagtgggaca ccaatcgaga ataatttaga agaattgtgg tcattgtttg caacgattat
    42901 gccaggattt ttcccgacta aaacaaaatt cagggaactt gccgcagaag aaattgcgca
    42961 aatgattcga ccgtttattt tacggcgaga taaacaaact gtgttgaaag acttgccgga
    43021 aaaaacagag atgaatctat attctgcact tacagaagaa caaaaaactg tttatttagc
    43081 ttacttgcgt caaatgcgtg aagaaatcac atcaatggat tctgaagcat tcaagaaaaa
    43141 ccggatcggg atacttgctg gtctgacaag actgcgacaa atttgctgcg atcctcgtct
    43201 gtttattgaa gattaccaag gcggttccgg caagctcgaa caagtaaaag atctactggt
    43261 agctgccaaa gagaacaaac gaagagtttt gctgttttct caatttacag ggatgctgac
    43321 tatcttgcaa gaagagttag ccgagctagg gatctcgaca ttttatttaa gaggcagtac
    43381 gaaaccacaa gatcggttaa gtatggtaga cgcatttaat gctggcgaaa aagatgtttt
    43441 tctgatttcc ttaaaggctg ggggaactgg attgaatctc acgggagcag atacggtcat
    43501 cttgtatgac ctttggtgga atcctgccat tgaagaacaa gctgctggac gtgcacatcg
    43561 gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa cgattgagga
    43621 acgcatggat tcactccagc aagaaaaacg cgaactgttc caaaaagtga tccaaggtaa
    43681 tgaagaacag ttgacgaaac tgactgaaga agacatccgc atgattttaa gtgtaggaga
    43741 aatcgaggag taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc ctttctaaag
    43801 aagcgattgt ttatttgatt taaaaaatag caaggaggct gggattccat tttatcccag
    43861 cttccttgct atcatttttt tatttctatt tttaaaggtt atacaaagat tgtcttcatt
    43921 gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc aaattggagt ctgttcaatt
    43981 ttgcgttctc gatggttgaa ccaaagcgct tgccagttag cactttttgc acctagaaca
    44041 tcattgtcgt aattatcgcc aacgtataag gtctcttctg gaagcatatg aaattctttc
    44101 tcagccagct gaaaaatctc tttttctggt ttttggaaac ctgttgcttg agaaatgagc
    44161 atattgcggg aaggaatcca attgttcaat tgaagttgct tcagtttttt tgtctgatgg
    44221 tcagtcggcc catttgtgat aattcctaat gggacattct tttccttcaa gaaatcgagg
    44281 gtttttttga cctcctcatg caagcagata ttatcaagtt cttcctcgta aattttttga
    44341 aataagaggc catcttcttc cgtaatatga gggtagtcta aatcttttag tgattggctg
    44401 atccgatgtg ctcgcatata ttctagtgtc caatctcctg ccattacttt tgggaagttt
    44461 tcatcactat gatggcggaa ccggatatat agtggatgca tatctgcatc tgaaacaagc
    44521 ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct gatcatacat cgtatcatca
    44581 acatcaaaca caactgattt aatcaaaatt ttacacgcct ttcggttttc attgattttt
    44641 tcacaaatcc ttgactattg taacagaaaa aattgtctaa aaaagctgat aaataaaggt
    44701 ttttcttact atatttctat ttccttgaca gaataaggtt tttatatttc agtttttttc
    44761 actaaaaatc gtgaaacacg tcaataagtt tgatgaacaa attaatcatt atggtagaag
    44821 tcgattgatt tattttaatg caatctctgt ggaattttcg aaagatttat
//
LOCUS       DAZKOM010000012        38658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000012 DAZKOM010000000
VERSION     DAZKOM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.012"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1454
                     /locus_tag="ACG8NF_000701"
     CDS             135..1454
                     /locus_tag="ACG8NF_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0471208.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1659..1946
                     /locus_tag="ACG8NF_000702"
                     /pseudo
     CDS             1659..1946
                     /locus_tag="ACG8NF_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2005..2445
                     /locus_tag="ACG8NF_000703"
     CDS             2005..2445
                     /locus_tag="ACG8NF_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471209.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            2457..3125
                     /locus_tag="ACG8NF_000704"
     CDS             2457..3125
                     /locus_tag="ACG8NF_000704"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0471210.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3189..3812
                     /locus_tag="ACG8NF_000705"
     CDS             3189..3812
                     /locus_tag="ACG8NF_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0471211.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            3868..4254
                     /locus_tag="ACG8NF_000706"
     CDS             3868..4254
                     /locus_tag="ACG8NF_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0471212.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            4467..5858
                     /locus_tag="ACG8NF_000707"
     CDS             4467..5858
                     /locus_tag="ACG8NF_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0471213.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            6469..6837
                     /locus_tag="ACG8NF_000708"
     CDS             6469..6837
                     /locus_tag="ACG8NF_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0471214.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7017..7958
                     /locus_tag="ACG8NF_000709"
     CDS             7017..7958
                     /locus_tag="ACG8NF_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0471215.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8027..8758
                     /locus_tag="ACG8NF_000710"
     CDS             8027..8758
                     /locus_tag="ACG8NF_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0471216.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            8902..11082
                     /locus_tag="ACG8NF_000711"
     CDS             8902..11082
                     /locus_tag="ACG8NF_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0471217.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11102..11584
                     /locus_tag="ACG8NF_000712"
     CDS             11102..11584
                     /locus_tag="ACG8NF_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0471218.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            11600..12361
                     /locus_tag="ACG8NF_000713"
     CDS             11600..12361
                     /locus_tag="ACG8NF_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0471219.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            12492..13826
                     /gene="murC"
                     /locus_tag="ACG8NF_000714"
     CDS             12492..13826
                     /gene="murC"
                     /locus_tag="ACG8NF_000714"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0471220.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14133..14618
                     /locus_tag="ACG8NF_000715"
     CDS             14133..14618
                     /locus_tag="ACG8NF_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0471221.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            14611..15294
                     /locus_tag="ACG8NF_000716"
     CDS             14611..15294
                     /locus_tag="ACG8NF_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0471222.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            15420..18065
                     /gene="polA"
                     /locus_tag="ACG8NF_000717"
     CDS             15420..18065
                     /gene="polA"
                     /locus_tag="ACG8NF_000717"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0471223.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18110..18946
                     /gene="mutM"
                     /locus_tag="ACG8NF_000718"
     CDS             18110..18946
                     /gene="mutM"
                     /locus_tag="ACG8NF_000718"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0471224.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            18910..19539
                     /gene="coaE"
                     /locus_tag="ACG8NF_000719"
     CDS             18910..19539
                     /gene="coaE"
                     /locus_tag="ACG8NF_000719"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0471225.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            19591..19923
                     /locus_tag="ACG8NF_000720"
     CDS             19591..19923
                     /locus_tag="ACG8NF_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0471226.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20235..20726
                     /gene="nrdR"
                     /locus_tag="ACG8NF_000721"
     CDS             20235..20726
                     /gene="nrdR"
                     /locus_tag="ACG8NF_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0471227.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            20749..22122
                     /locus_tag="ACG8NF_000722"
     CDS             20749..22122
                     /locus_tag="ACG8NF_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0471228.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22122..23048
                     /gene="dnaI"
                     /locus_tag="ACG8NF_000723"
     CDS             22122..23048
                     /gene="dnaI"
                     /locus_tag="ACG8NF_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0471229.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23177..23617
                     /locus_tag="ACG8NF_000724"
     CDS             23177..23617
                     /locus_tag="ACG8NF_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0471230.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            23761..24462
                     /locus_tag="ACG8NF_000725"
     CDS             23761..24462
                     /locus_tag="ACG8NF_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0471231.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            24459..25580
                     /locus_tag="ACG8NF_000726"
     CDS             24459..25580
                     /locus_tag="ACG8NF_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0471232.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            25595..26827
                     /gene="pepT"
                     /locus_tag="ACG8NF_000727"
     CDS             25595..26827
                     /gene="pepT"
                     /locus_tag="ACG8NF_000727"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0471233.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            26959..27867
                     /locus_tag="ACG8NF_000728"
     CDS             26959..27867
                     /locus_tag="ACG8NF_000728"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0471234.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            27902..28174
                     /locus_tag="ACG8NF_000729"
     CDS             27902..28174
                     /locus_tag="ACG8NF_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0471235.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28185..29231
                     /locus_tag="ACG8NF_000730"
     CDS             28185..29231
                     /locus_tag="ACG8NF_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0471236.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            29482..32091
                     /gene="clpB"
                     /locus_tag="ACG8NF_000731"
     CDS             29482..32091
                     /gene="clpB"
                     /locus_tag="ACG8NF_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0471237.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32242..32928)
                     /locus_tag="ACG8NF_000732"
     CDS             complement(32242..32928)
                     /locus_tag="ACG8NF_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471238.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(32906..33400)
                     /locus_tag="ACG8NF_000733"
     CDS             complement(32906..33400)
                     /locus_tag="ACG8NF_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0471239.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(33420..34640)
                     /locus_tag="ACG8NF_000734"
     CDS             complement(33420..34640)
                     /locus_tag="ACG8NF_000734"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0471240.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            34787..36622
                     /gene="lepA"
                     /locus_tag="ACG8NF_000735"
     CDS             34787..36622
                     /gene="lepA"
                     /locus_tag="ACG8NF_000735"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0471241.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            36818..37729
                     /locus_tag="ACG8NF_000736"
     CDS             36818..37729
                     /locus_tag="ACG8NF_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471242.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        13015 a         6727 c         8224 g        10692 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacgaac
      301 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
      361 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
      421 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
      481 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
      541 tcatccgtaa tgatacggaa ttaatcattg atttagattc gacccattca gatacatttg
      601 gtcaccaaga acaaacgaat tataatacac actaccaaac ctatggttac catccattgg
      661 tagcttttga cggattgact ggtgacttct taaaagctga actacgttca ggaaatcaat
      721 atacatcaaa aggcgtaaag gagtttctca cacctttatt agagcactat aatcactctc
      781 taccaaacac tgacatcttg gttcgtggag acagcgggtt cgctacacct ggtgtgtatg
      841 attcgtgtga atcaaaaaag agtcattatg ttattcgact gaagaataat cgtagactag
      901 gtcaaatagc tgagaaatca gtactttatg gcgataatca aaagtgggaa gaacgagaag
      961 ttcagtactt ctcgaccact tatcaagcac aatcctggtc acaaagtcgt cgcgtgtgta
     1021 tacgctcaac acgtgaagcg ggcgaactac tctttcgaca tgaatttatc gtgacgaatc
     1081 tatcagaaaa tgtttctcct gataccatct tttctatcta tgccaaacgt ggcacaatgg
     1141 agaacttcat taaagaagcg aaagctggct tttactttga caaaacagac agtcctcgct
     1201 ttttggagaa tcatgtccga atgatgctca gcttgatagc ttacaactta gtcaacttct
     1261 taagaacgat tggctttgaa gaagtccaaa agggaatgac cattcattct attcgattga
     1321 agtttctgaa agttgctggg aaattagtcc aaacgggtag acgagtctat ctcaaattat
     1381 ctagttatca tgtgtatcag aatgaattct acagggtctt tgctcgcctg aggcgagcca
     1441 gtcaatggat ctaatccaac aatctaacga tttttttact gggaagttat ccagggagaa
     1501 gtatgctcaa aatgccttac acccaaaatt tattcctcaa tatttttgaa cagtgaatgt
     1561 ttgaagtaac tttttagtca aaatcagttg atagggatat tagtgataca tatttcaata
     1621 aaatgtgcca aagaattaaa gctatgaatt attcaggtct aaaaagcttt ttaaaagtga
     1681 tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat ccaggtctgc
     1741 ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt atacgtatgc
     1801 aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga gtagtcaaca
     1861 gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat gtccttcacc
     1921 aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt gtcataataa
     1981 atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct aatacgattt
     2041 cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca gttgaatcgc
     2101 ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc ctatagaatc
     2161 ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct atcaaaaaaa
     2221 atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag agtcaaagaa
     2281 aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag ttatactttt
     2341 tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg acgaatacgt
     2401 gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag aaaagaatga
     2461 aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac tggacaggat
     2521 ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc ggtcaaaaaa
     2581 tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa cgtgcgattc
     2641 ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata ataaaaacat
     2701 ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat aagatgaaag
     2761 aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa gttccgccgt
     2821 taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat ttgatcccat
     2881 acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa gatcatctag
     2941 caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat agcatccgag
     3001 cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc gatatcccaa
     3061 atgcacaacc aatccagtat acattagcag atgatttgac aattctggac aaaaaaatac
     3121 tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag gaatggaaca
     3181 aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat acgtatcccg
     3241 caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg gacaaaaaag
     3301 aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa tcaaacttga
     3361 atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg gaagtttccc
     3421 gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc ggtcgtcatt
     3481 tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa catcgattgg
     3541 cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa cctgatccag
     3601 catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg atgatgatcg
     3661 gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact tgtctttacg
     3721 acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat ctgaatgaaa
     3781 tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag atatgttata
     3841 attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga agttgtagaa
     3901 gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt gaaagtaggc
     3961 ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat cgtaggagca
     4021 cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt gagtcagatc
     4081 aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga agaagtggac
     4141 gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt gacagagatc
     4201 gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga atagacagta
     4261 aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta ctaattgtaa
     4321 gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact gaaaaaaatt
     4381 ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc gatggacttg
     4441 tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta gtggttctag
     4501 tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca aaattttcaa
     4561 cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt caattgatct
     4621 ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt gtaggtcaag
     4681 gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg atcattggtg
     4741 cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact gtattgactt
     4801 ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca atgctgttcg
     4861 gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc tctgaactgg
     4921 caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta tcctttatcc
     4981 ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt ggagtcgtta
     5041 ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg ccaaaagaag
     5101 cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg atcgtgacgc
     5161 ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt ttggcaacaa
     5221 gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac gtagcattgc
     5281 ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat ccaatcaact
     5341 atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca agtgctggag
     5401 ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat gcaacagcta
     5461 atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc gtttatccag
     5521 ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt ttggaattta
     5581 ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga gtcggtgggg
     5641 gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta gcaatcgttg
     5701 ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta aacgtcagcg
     5761 gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat cgcgatgtca
     5821 tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt tgtgatagaa
     5881 gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa atttttgtga
     5941 aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag gttgtgaaaa
     6001 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttacgt
     6061 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg
     6121 agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt ctcatgtttg
     6181 ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac cagaggttgt
     6241 gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca cattatttat
     6301 tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt aaagaaaagc
     6361 gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt tcctatggta
     6421 aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat gtttacgaat
     6481 gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt ttggagagca
     6541 gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat tgaagtagga
     6601 gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga gtcccattca
     6661 cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat ctttggacta
     6721 tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct tggagaagaa
     6781 tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg taaataatag
     6841 aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta attcaaataa
     6901 taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa taaatcgtca
     6961 aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag aaatacatgg
     7021 attatcaagt actgctttat tacaattata caaaaatcga agaaccagag gaatttgcca
     7081 aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta gtagctgaag
     7141 aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat atgaaagcaa
     7201 tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc gaaggacatg
     7261 cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag ctagaagagg
     7321 acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt aaagaagcta
     7381 ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat gacttgggac
     7441 attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca gactggatcc
     7501 gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact ggtggcattc
     7561 gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc ggacagctgc
     7621 atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta tgggacggca
     7681 agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat aaacaaatcg
     7741 tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt agcaatcctg
     7801 agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta ggagcatgta
     7861 gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga atctctccag
     7921 aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt ttgattgtcg
     7981 accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga aatcattgat
     8041 ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta ttcactatta
     8101 tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag gtcctcgtac
     8161 aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca tttacctccg
     8221 gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact ggtatgcgcc
     8281 ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag gggcatcgga
     8341 ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag aactgattaa
     8401 atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg tccatgccgg
     8461 tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta tttggataag
     8521 ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg gacatacgat
     8581 tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg atcataaaat
     8641 cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt ttgacaagga
     8701 aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg gaatctgact
     8761 aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt atagagaata
     8821 ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag aagcaatttt
     8881 tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat tcagcttgta
     8941 caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa tttgttaaat
     9001 gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg aagtcttgat
     9061 gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt agaaaaacgt
     9121 aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga gttagaagca
     9181 gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc atacaaacaa
     9241 aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact cgcgaagtgg
     9301 ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat tgacaaagaa
     9361 aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat tgctgaacaa
     9421 gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa caaaggtatg
     9481 tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga aatgtattat
     9541 gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac taatcgtgga
     9601 gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat tttagcttac
     9661 ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt cagagaagcc
     9721 tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact gcgcaatgaa
     9781 ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct ccgtaatctt
     9841 ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc ttatcgtact
     9901 ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga agttatttat
     9961 cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt tatccaatta
    10021 attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag tcgggaatct
    10081 gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta cgctatcgtc
    10141 aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga atttccgcat
    10201 ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga tcctttagct
    10261 gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca tgacgtttct
    10321 caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa ccaagtaggt
    10381 gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt gaataaaaca
    10441 actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc acgaactcaa
    10501 ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg attcttgcga
    10561 gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag ttattctatt
    10621 gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac agaagaagca
    10681 gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc agtcggagaa
    10741 gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat gcgtgatgag
    10801 atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa accagggatg
    10861 gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga tatcggtgtg
    10921 aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa gcatcctaca
    10981 gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga tacgaaaaaa
    11041 ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg agtaacaaaa
    11101 attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa gcttccagct
    11161 tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg ggcgctatca
    11221 tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag acgaagagac
    11281 gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag ctggaagagg
    11341 gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag ggttgcgcta
    11401 tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc aagattgttc
    11461 tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg gaagatgtca
    11521 tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac aaaaaatcag
    11581 atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag actggaatca
    11641 gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga cggcacctac
    11701 acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac aaatgtattt
    11761 aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga ctagaatcct
    11821 ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg aaagtgaaat
    11881 ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc tgatagaatc
    11941 gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc attccttagg
    12001 agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca ggactcgagc
    12061 aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg tgccatttgt
    12121 gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac tgaatcatca
    12181 atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc tccatgacga
    12241 tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata tatcagagga
    12301 agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt ctcgtacttg
    12361 accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt tcaatcgttt
    12421 ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct agataagaga
    12481 agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc aaaggttcag
    12541 gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa ggatcagaca
    12601 ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg attctgccat
    12661 ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca tttcctgatt
    12721 cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc tatcacgact
    12781 ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct cacggaaaaa
    12841 caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca agttatctaa
    12901 ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt gaggcctgtg
    12961 aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc aatatcgatt
    13021 ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc caaacaatgg
    13081 ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta cgcaaattaa
    13141 aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc caagcacgaa
    13201 acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat gaatttgttg
    13261 ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta ggtgttattg
    13321 cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg ctgactttcc
    13381 caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta gtcgatgatt
    13441 atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa aaatatccag
    13501 ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt gcattgatgg
    13561 atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat atttttggtt
    13621 cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa atcaaaaaag
    13681 gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat gatgcagtag
    13741 taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa aaacttttaa
    13801 gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata aaaatagaaa
    13861 gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga taaacggtgt
    13921 tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag aagctgtttt
    13981 ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct gtaaactata
    14041 ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg tacaataatg
    14101 tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg agaaaactag
    14161 gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa tctattgaag
    14221 ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg tatatccaac
    14281 atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg atgctgatgg
    14341 ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat gttgtcaatt
    14401 tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt cagtttgagg
    14461 tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg gagaatacga
    14521 acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt cctgaattgg
    14581 aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc aatgtcaggt
    14641 atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc ccttagtgca
    14701 gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt tatcaataat
    14761 tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat ggttctaggc
    14821 gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta ctctcttttg
    14881 aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt aacgcaagct
    14941 ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac aacaaaaaga
    15001 gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat cattgcacta
    15061 gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc cctactgtta
    15121 gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa tatctatgtc
    15181 cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa gctgtatttg
    15241 gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa ctaaaaaaga
    15301 aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct ttttcttttc
    15361 gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga gttaagaaga
    15421 tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc gcattctttg
    15481 cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca aatgcaatct
    15541 atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact catgttctgg
    15601 ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag tacaaaggtg
    15661 gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt gatttgttga
    15721 caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat attatcggta
    15781 ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca ggtgatcggg
    15841 atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa ggtgtcagcg
    15901 atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg acgccaaaac
    15961 agattattga catgaaggga ctagccggag atgcctctga caatatcccc ggtgtgacta
    16021 aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc gaaggaattt
    16081 atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata aatgacaaag
    16141 agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct gtggatgtct
    16201 ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg ttttataaag
    16261 aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa gtaaaaatgg
    16321 atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact acggatatgg
    16381 cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc ggagtagcgt
    16441 gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa aaccgagcgt
    16501 tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg aaacgaactt
    16561 atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat gtcttgctag
    16621 gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata gctgctcatt
    16681 acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg aaaaaaggtt
    16741 taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct gcaatcaatg
    16801 cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat cttttccgga
    16861 aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg atcaaagtag
    16921 atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga gagatcgaac
    16981 aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag caattaggag
    17041 tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact ggatattcca
    17101 cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag gatatcctca
    17161 cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg aaagtgatcc
    17221 aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca ggacgattaa
    17281 gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg agaaagatac
    17341 gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac tattcacaaa
    17401 tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct gccttcctag
    17461 aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa aaggcagagg
    17521 atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc gtgtatggca
    17581 tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca cagcaataca
    17641 tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga atcgtacgcg
    17701 aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat ctgccagaca
    17761 tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc aatacaccaa
    17821 tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat aaacgattaa
    17881 aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt gtcttcgagg
    17941 taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg gaacaagctg
    18001 tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg tacgaagcaa
    18061 aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa tgccagaatt
    18121 accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa aaacgattca
    18181 aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca tctttgaggc
    18241 ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt taatttttca
    18301 tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc aatttacgaa
    18361 agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg atggaagcca
    18421 acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa aaggagcaag
    18481 tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata gcttcttatt
    18541 agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat tgctgctaga
    18601 ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat gggaagcgaa
    18661 gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac aattacgtct
    18721 atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga ttcgcagtta
    18781 tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct atcagcaaac
    18841 tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg cacaaagggg
    18901 gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt tgttttaggt
    18961 ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca gtctcatgga
    19021 tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg aacgcttgct
    19081 ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg gcaactagat
    19141 cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa acttgatcag
    19201 ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct aagttccaat
    19261 catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga ccactatatg
    19321 gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt gatgaaacgt
    19381 aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc aatcgaagaa
    19441 aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa acttgtggga
    19501 caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc aattttgcta
    19561 tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca aaaggataaa
    19621 gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa tctaatcgat
    19681 gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca agcaagaatc
    19741 gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt gactcaagaa
    19801 cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga ccacattccc
    19861 gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga agagaagtta
    19921 taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc atctgcttca
    19981 agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca gtaatcaagg
    20041 tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac gcatgaaagt
    20101 agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt gtgatttgtt
    20161 tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa atgcgatgca
    20221 agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga gtaatcgaca
    20281 gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag aattgttcgt
    20341 tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc aagaaaaacg
    20401 gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt tcagcagaaa
    20461 aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa aaccgcgtac
    20521 gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg atggaagatt
    20581 tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc cagttcaaag
    20641 atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa gaaaataata
    20701 aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt ggaaaatgct
    20761 tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc gctgagagaa
    20821 gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga agccttgagt
    20881 atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc tgaagggatg
    20941 catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga tgcaagaaaa
    21001 aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga tctgggacct
    21061 cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa agactctgtt
    21121 ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct tacccaattc
    21181 tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa atttttagat
    21241 gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga atcgctgaat
    21301 aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac attcgattgg
    21361 cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga tccaatcatc
    21421 aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact tggcgaactg
    21481 atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact gcaaaaagcc
    21541 attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc ggtttctgaa
    21601 aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca gctgataaaa
    21661 gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga aaaaggcgga
    21721 tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc aggtctttcc
    21781 agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa tgctagctta
    21841 aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat cgccacagca
    21901 gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc aaaacaaaag
    21961 aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct tcctgattgg
    22021 gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga gattgaccgc
    22081 cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac gtaggcaaag
    22141 aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa atgatcgctg
    22201 aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa gaattgacac
    22261 aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa aagcgtaaat
    22321 atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg acgttgaatt
    22381 ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg caaagacaag
    22441 aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa tcggcttctt
    22501 ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc ctagattttg
    22561 tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta gtaggaagtt
    22621 ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg attgctggat
    22681 tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag gcaatcggaa
    22741 aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg atgatcgatg
    22801 acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt gttatccttc
    22861 aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca atggatgaat
    22921 tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa gcgaaacgga
    22981 taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc aaccgacgca
    23041 atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag caggtcagcg
    23101 aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat gcagaaactt
    23161 aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg gtctattttt
    23221 tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt cgattgatgc
    23281 gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat ttgtcggaac
    23341 gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga aactcgtcga
    23401 tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg acggagtgat
    23461 cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa tcggtgacaa
    23521 agtccaattt acattagttg agatgccgat catgacgatg tctatcccac cgcaagttcc
    23581 tggagattcg attattgatg tttcattgaa caaataacca ataacatttt ttcaaaaata
    23641 agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg ggaatttttt
    23701 tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag gagtgaagct
    23761 atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt ccctgaatcc
    23821 gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt gatattaaga
    23881 ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga atcagcacgt
    23941 aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc aaatggtcta
    24001 gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat gggaggttca
    24061 ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg acaagaacgt
    24121 ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca agaaaatcag
    24181 tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga aatcattgta
    24241 gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt cgggccattt
    24301 ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct tcaacaaaga
    24361 aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca aaaacaaatc
    24421 gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc ggacgcactt
    24481 tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga gacccagtcg
    24541 gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca ttagacgttc
    24601 gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt gctaaacatc
    24661 ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa aaaatgtatg
    24721 cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg gatattattt
    24781 gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag agttatttag
    24841 taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg gatcatgcac
    24901 agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat tataccggta
    24961 caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca caaccagcga
    25021 ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt attttgccgg
    25081 aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat gaggaacctg
    25141 cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga cgagtaggtg
    25201 aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa gcgttccaac
    25261 tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag cgcattgcga
    25321 tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga gcagatgtct
    25381 atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct gggttgattg
    25441 caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc gagaaatttg
    25501 aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa acaaatacaa
    25561 acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt tgccacgttt
    25621 tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga ctccatcgac
    25681 tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt taggcttagc
    25741 tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa gcaatgttga
    25801 tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt ttaatgcagt
    25861 aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg tactagatgc
    25921 ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt atgaaggcca
    25981 cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat cagggattgc
    26041 ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc acggtaccat
    26101 caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat tcgatgttga
    26161 gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg agttgcagta
    26221 tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg tacatccagg
    26281 gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc ataatcagct
    26341 tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc atttgatggc
    26401 attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc atgatcgtga
    26461 aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga atcagcgatt
    26521 tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga aagaaatcat
    26581 tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc ttggtatctc
    26641 tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt acatgggtat
    26701 tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg aatttgtttc
    26761 tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat tgaacacaaa
    26821 gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga tggaatgtca
    26881 tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct tttttgaaga
    26941 gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag ttcagggaaa
    27001 gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa gcagccagaa
    27061 ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt agtcaagaaa
    27121 gcagaagcca tagaagctga tactttagta attataggtg gagacggcac gattcatcat
    27181 atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact tccaggtgga
    27241 actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga agctgctgac
    27301 gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga tgaagtcatt
    27361 atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag tcaagaagaa
    27421 aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt gctgaagaag
    27481 aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa atcccaactg
    27541 ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga ctcagcgacc
    27601 ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt ccgatttatt
    27661 ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat tacttatttc
    27721 acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg aacggatgga
    27781 gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt aacagtttat
    27841 tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg gaagaagagt
    27901 aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga atttttactt
    27961 attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc gtgttcctga
    28021 gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga tctctcgaaa
    28081 agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg caggtgtact
    28141 tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg tctaaaggaa
    28201 cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc attatttatt
    28261 atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc agtgttgttg
    28321 ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg cgctcaatca
    28381 aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc ttgacctcta
    28441 gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc tcggagattt
    28501 atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca gcactccata
    28561 tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc atccaattct
    28621 ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt atccttttga
    28681 cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt caaatatttt
    28741 ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg gtggatacat
    28801 gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa gacaggactg
    28861 cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat gtacttcctt
    28921 atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt tcgtttatca
    28981 gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt tcctttgaat
    29041 ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt tcttcgcttt
    29101 tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg tatgtgattg
    29161 ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag aataaagaaa
    29221 atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa aaacgttatg
    29281 gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc aattttgttt
    29341 ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata ttttgaccaa
    29401 aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg atcttacctt
    29461 taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg
    29521 attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat
    29581 gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac ggatgccgga
    29641 ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt
    29701 tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat
    29761 gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt
    29821 ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta
    29881 tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca
    29941 caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa
    30001 gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc
    30061 cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg
    30121 aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat
    30181 ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc
    30241 cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga
    30301 cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga
    30361 agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc
    30421 ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt
    30481 cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt
    30541 ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta
    30601 gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc
    30661 gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa
    30721 ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa
    30781 gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt
    30841 cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct
    30901 gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat
    30961 aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa
    31021 gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc
    31081 gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa
    31141 cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg
    31201 attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga
    31261 ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga
    31321 aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta
    31381 cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct
    31441 ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct
    31501 tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg
    31561 ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa
    31621 aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact
    31681 gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac
    31741 ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta
    31801 gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat
    31861 caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat
    31921 gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca
    31981 ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta
    32041 cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt
    32101 gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat
    32161 aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg
    32221 cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat aactttcaaa
    32281 gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat
    32341 cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt
    32401 gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc
    32461 ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa
    32521 ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc
    32581 aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat
    32641 actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa
    32701 caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa
    32761 aatcaatgca atccctaata aagattccac gataaatgtg attccttgag aagtcgaata
    32821 tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa
    32881 agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt
    32941 ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac
    33001 ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga
    33061 tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga
    33121 tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat
    33181 ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca
    33241 ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga
    33301 tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat
    33361 cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat
    33421 caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt
    33481 gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc
    33541 atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt
    33601 aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga
    33661 gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt
    33721 tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg
    33781 atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc
    33841 cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga
    33901 tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta gaacagaaca
    33961 cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg
    34021 aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg
    34081 gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata
    34141 ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca
    34201 gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata caaataagag
    34261 atccattgct tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat
    34321 ccatggttag catattctgg ataagttcca actccagtca caccacccat tttataacgt
    34381 gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg
    34441 tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct
    34501 cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa
    34561 agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct
    34621 ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa
    34681 aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt
    34741 cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa
    34801 aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg
    34861 gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat
    34921 gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt
    34981 aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat
    35041 tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga
    35101 gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt
    35161 ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc
    35221 cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag
    35281 tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat
    35341 cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt
    35401 tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat
    35461 ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga
    35521 ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg
    35581 ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt
    35641 agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta
    35701 ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa
    35761 actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt
    35821 tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga
    35881 acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa
    35941 aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat
    36001 tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg
    36061 tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga
    36121 tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt
    36181 cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta
    36241 tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt
    36301 aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact
    36361 gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa
    36421 aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg
    36481 tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat
    36541 gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga
    36601 tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa taaacctttt
    36661 tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc atttggttat
    36721 cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt tagctgtcag
    36781 tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt caagtggaag
    36841 ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag ctgatttagc
    36901 aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa tccctaaaga
    36961 cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag atgaggaaga
    37021 taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata agttacttaa
    37081 caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg tgttaaatag
    37141 aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat taagtaatga
    37201 aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat caaaagacga
    37261 taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag agacgcttga
    37321 aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta gatgggaaaa
    37381 aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg gtaaggtcac
    37441 tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag atgaaagagt
    37501 tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga aaattaggaa
    37561 agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa tttcaaaatg
    37621 gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa ttatccaata
    37681 tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa aatagtttac
    37741 gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata aaaggaggtt
    37801 tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg cagctttttt
    37861 ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat acaagaacta
    37921 actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca atttagtaat
    37981 cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga tttgtagtgt
    38041 ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag cgtcaggatg
    38101 ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg ttaatatgtc
    38161 agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct tatatgagaa
    38221 aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt tctggaaaag
    38281 taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa aaacggcatc
    38341 gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt
    38401 ctatttatct ttttacatct atcgtttttt caagtagccg caaattatat cagcattcaa
    38461 caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga
    38521 taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt aggttaggac
    38581 aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag taatttccga
    38641 ttattggagt ttttttat
//
LOCUS       DAZKOM010000013        37146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000013 DAZKOM010000000
VERSION     DAZKOM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.013"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG8NF_000737"
     CDS             complement(<1..384)
                     /locus_tag="ACG8NF_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471243.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG8NF_000738"
     CDS             complement(569..967)
                     /locus_tag="ACG8NF_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0471244.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG8NF_000739"
     CDS             complement(964..1737)
                     /locus_tag="ACG8NF_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0471245.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG8NF_000740"
     CDS             complement(1734..2417)
                     /locus_tag="ACG8NF_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0471246.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG8NF_000741"
     CDS             complement(2584..3471)
                     /locus_tag="ACG8NF_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471247.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG8NF_000742"
     CDS             complement(3468..4337)
                     /locus_tag="ACG8NF_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471248.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG8NF_000743"
     CDS             complement(4474..5760)
                     /locus_tag="ACG8NF_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0471249.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ACG8NF_000744"
     CDS             6033..6611
                     /locus_tag="ACG8NF_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0471250.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ACG8NF_000745"
     CDS             6611..7540
                     /locus_tag="ACG8NF_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471251.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ACG8NF_000746"
     CDS             7537..9273
                     /locus_tag="ACG8NF_000746"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0471252.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG8NF_000747"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG8NF_000747"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0471253.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ACG8NF_000748"
     CDS             complement(10169..10678)
                     /locus_tag="ACG8NF_000748"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0471254.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG8NF_000749"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG8NF_000749"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0471255.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ACG8NF_000750"
     CDS             complement(11979..13070)
                     /locus_tag="ACG8NF_000750"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0471256.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG8NF_000751"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG8NF_000751"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0471257.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG8NF_000752"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG8NF_000752"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0471258.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG8NF_000753"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG8NF_000753"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0471259.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="ACG8NF_000754"
     CDS             complement(17033..18007)
                     /locus_tag="ACG8NF_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0471260.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="ACG8NF_000755"
     CDS             complement(17988..19871)
                     /locus_tag="ACG8NF_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0471261.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="ACG8NF_000756"
     CDS             complement(19889..22114)
                     /locus_tag="ACG8NF_000756"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0471262.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="ACG8NF_000757"
     CDS             22229..23263
                     /locus_tag="ACG8NF_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0471263.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="ACG8NF_000758"
     CDS             complement(23330..24343)
                     /locus_tag="ACG8NF_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0471264.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="ACG8NF_000759"
     CDS             complement(24421..24609)
                     /locus_tag="ACG8NF_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0471265.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="ACG8NF_000760"
     CDS             complement(25049..25177)
                     /locus_tag="ACG8NF_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0471266.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG8NF_000761"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG8NF_000761"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0471267.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26664..27719)
                     /locus_tag="ACG8NF_000762"
     CDS             complement(26664..27719)
                     /locus_tag="ACG8NF_000762"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0471268.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG8NF_000763"
     CDS             complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG8NF_000763"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0471269.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG8NF_000764"
     CDS             complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG8NF_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0471270.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG8NF_000765"
     CDS             complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG8NF_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0471271.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG8NF_000766"
     CDS             complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG8NF_000766"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0471272.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG8NF_000767"
     CDS             complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG8NF_000767"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0471273.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35481..37040
                     /locus_tag="ACG8NF_000768"
     CDS             35481..37040
                     /locus_tag="ACG8NF_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0471274.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10262 a         8066 c         6697 g        12121 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagcta attacacaac taaaaccggc actgacctta
    26641 ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc tgctattttt
    26701 ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa ttctttgctg
    26761 acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat ctgatcggta
    26821 ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg
    26881 tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt ttcaacgaac
    26941 aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc gccattcagc
    27001 atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat gattaaagaa
    27061 aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc aacagagttc
    27121 gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc atacttcaag
    27181 cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag ataagcaaga
    27241 taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt caaatagcca
    27301 cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat ttttacggtt
    27361 tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat ttttaatgct
    27421 cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg gtgttcaagc
    27481 ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc cactactaga
    27541 tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg ttctggtgta
    27601 gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat atccgccaaa
    27661 ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt
    27721 ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac accaggattt
    27781 gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc acgatctttt
    27841 ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt aatgattggc
    27901 agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac ttcttgggct
    27961 tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga gctgatcgtt
    28021 ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt tgtctcagct
    28081 aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca gttagatgct
    28141 tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta
    28201 agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata gcgttcgcga
    28261 cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc gtcatcgatc
    28321 acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc ttctttgaca
    28381 cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct
    28441 cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg
    28501 aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc ttgtccataa
    28561 gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt cacatcgctg
    28621 acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc ttcttctggt
    28681 gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca
    28741 acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc taaatggaca
    28801 cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca tccgtcatga
    28861 ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg gtaaaagtaa
    28921 tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa agctgctttc
    28981 atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata accccagtcg
    29041 atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg agagaaaatt
    29101 tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc aaaattcatt
    29161 cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct tgttcaaacg
    29221 catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc tgcaatccaa
    29281 ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca gccaagttga
    29341 cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga
    29401 ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct gcaaagactt
    29461 tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag taagcgtcat
    29521 aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact tcttcaccga
    29581 atccttctga acgtgagttg acatatacat catctaatga tttcacattt tcagagcgat
    29641 aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat gaggcaatga
    29701 tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc acaacagcac
    29761 ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca ggggccacac
    29821 cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc
    29881 cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg tcataaccgc
    29941 tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt ccgatttggt
    30001 caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa gtaggtttca
    30061 tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta tcactaccta
    30121 aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa gagccaccag
    30181 gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt tctgtacttc
    30241 cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct gcttgataga
    30301 ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc ttagatgcag
    30361 cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga ataccagcaa
    30421 ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc aatgcttttt
    30481 cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt cgttcaaatg
    30541 ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga tgcagttctt
    30601 ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat cgatgattgc
    30661 gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt catcgcggtt
    30721 ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca cattcgacgt
    30781 aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga tgattttacc
    30841 taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg ctaatttggc
    30901 aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta aacgagttaa
    30961 aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta ttgaaatttg
    31021 tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg catctgccat
    31081 ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct tgctgccagc
    31141 atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca ctttcccgcc
    31201 aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt taccagtaag
    31261 gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat ttcgaagccc
    31321 tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat ggacttcttc
    31381 gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat caagcgcaat
    31441 aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat
    31501 ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc gctcgaccga
    31561 attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt cttttatttt
    31621 gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat ctgctactag
    31681 ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat tcatcgcatt
    31741 tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag gattgatgca
    31801 ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg gacaatgtgt
    31861 aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt gcgaaacttc
    31921 tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga tatccttcaa
    31981 cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg ctactcggac
    32041 tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc attcgatgtt
    32101 ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc atctcggtgc
    32161 tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga tgacgattcc
    32221 atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt atgaccacac
    32281 ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa ttttttctag
    32341 ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag ctactgtgta
    32401 taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac gcaaagaacc
    32461 agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc tgtcttggtt
    32521 caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga tgttcattgg
    32581 ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga tgttttctcc
    32641 tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat aacgcaaaga
    32701 aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc ccactgcttt
    32761 ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta
    32821 aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc ctactcgttg
    32881 agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta ctaattctgc
    32941 gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt agtattcgcg
    33001 agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt
    33061 catttcatgt tccatcattt accctccact actaagcttt ttcaattggc gcaaaggcag
    33121 ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga tccttcgacg
    33181 tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact ttatctccag
    33241 gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc gctgtctctg
    33301 gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta ggcgtaggac
    33361 gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga gaaggtcggt
    33421 tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac aacgcttctt
    33481 ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg ctttcttcca
    33541 tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat acaactggaa
    33601 attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta
    33661 aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt ttttcttctg
    33721 gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg
    33781 ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt tggattttta
    33841 agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc actgtcaagt
    33901 atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat tgctgtcctg
    33961 cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt ggccattcat
    34021 gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct cgtttgggcg
    34081 aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca ttcaaatacg
    34141 ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc attttataag
    34201 gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca
    34261 agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc atccggtcta
    34321 cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc ttgtttcctt
    34381 ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca atgacttggt
    34441 ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa attactgcag
    34501 catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg cgccagccgt
    34561 aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct gccaataaat
    34621 tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga atataatgga
    34681 atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga atcgtattca
    34741 tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg taagcagcat
    34801 aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg
    34861 tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga tcatattttt
    34921 tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat
    34981 cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt aaaatacgca
    35041 cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg agaatcgcat
    35101 tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct aggatgttcc
    35161 aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct
    35221 ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga acagcttctt
    35281 tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac aacatccttt
    35341 cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta gctagttcaa
    35401 aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg cgctaaatta
    35461 aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta gataagactc
    35521 agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc gtcgttagcc
    35581 tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat gaaatgagtc
    35641 gggaacagcc gatttactta attggcctag ctatttgctg catccttgca gcatttttcg
    35701 tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca caagtagtgg
    35761 gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa aagttcattg
    35821 gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct tctatacagc
    35881 ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct tcagaagaaa
    35941 agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat gctcctatcg
    36001 caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt gtttggctga
    36061 catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc ggcttgagac
    36121 ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt tccttagtga
    36181 tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg ctggctttgc
    36241 caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt cctttcttgc
    36301 tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat caaatcgttt
    36361 tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt gttcttcgat
    36421 tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc cttccgatat
    36481 tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat ttcgggatgg
    36541 atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc acagcgattg
    36601 gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct cttaatcatc
    36661 tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt ctcggcggta
    36721 atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc gcctcactaa
    36781 aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta gacggattaa
    36841 tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc agaggaaatc
    36901 aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg gttatcttga
    36961 ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct tctatcacaa
    37021 atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact attcatctac
    37081 actacttgat tctatatgat tctataaaaa aagaagaggc tgggaaaacc cagcctcttc
    37141 tttttt
//
LOCUS       DAZKOM010000014        36651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000014 DAZKOM010000000
VERSION     DAZKOM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.014"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..3308
                     /locus_tag="ACG8NF_000769"
     CDS             30..3308
                     /locus_tag="ACG8NF_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0471275.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3849..5756)
                     /locus_tag="ACG8NF_000770"
     CDS             complement(3849..5756)
                     /locus_tag="ACG8NF_000770"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0471276.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5886..6761
                     /locus_tag="ACG8NF_000771"
     CDS             5886..6761
                     /locus_tag="ACG8NF_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0471277.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6841..7815
                     /locus_tag="ACG8NF_000772"
     CDS             6841..7815
                     /locus_tag="ACG8NF_000772"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0471278.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7995..9140
                     /locus_tag="ACG8NF_000773"
     CDS             7995..9140
                     /locus_tag="ACG8NF_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0471279.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9277..9621
                     /locus_tag="ACG8NF_000774"
     CDS             9277..9621
                     /locus_tag="ACG8NF_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0471280.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9892..11178
                     /gene="efmA"
                     /locus_tag="ACG8NF_000775"
     CDS             9892..11178
                     /gene="efmA"
                     /locus_tag="ACG8NF_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0471281.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11316..11915)
                     /locus_tag="ACG8NF_000776"
     CDS             complement(11316..11915)
                     /locus_tag="ACG8NF_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0471282.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12319..13440
                     /gene="mnmA"
                     /locus_tag="ACG8NF_000777"
     CDS             12319..13440
                     /gene="mnmA"
                     /locus_tag="ACG8NF_000777"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0471283.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13686..15107
                     /gene="arcD"
                     /locus_tag="ACG8NF_000778"
     CDS             13686..15107
                     /gene="arcD"
                     /locus_tag="ACG8NF_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0471284.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15121..16239
                     /locus_tag="ACG8NF_000779"
     CDS             15121..16239
                     /locus_tag="ACG8NF_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0471285.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16299..17591)
                     /locus_tag="ACG8NF_000780"
     CDS             complement(16299..17591)
                     /locus_tag="ACG8NF_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0471286.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17741..18301
                     /locus_tag="ACG8NF_000781"
     CDS             17741..18301
                     /locus_tag="ACG8NF_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0471287.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18430..18807
                     /locus_tag="ACG8NF_000782"
     CDS             18430..18807
                     /locus_tag="ACG8NF_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0471288.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18740..20812
                     /locus_tag="ACG8NF_000783"
     CDS             18740..20812
                     /locus_tag="ACG8NF_000783"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0471289.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21023..21970)
                     /locus_tag="ACG8NF_000784"
     CDS             complement(21023..21970)
                     /locus_tag="ACG8NF_000784"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0471290.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22216..22680
                     /locus_tag="ACG8NF_000785"
     CDS             22216..22680
                     /locus_tag="ACG8NF_000785"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0471291.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22742..23785)
                     /locus_tag="ACG8NF_000786"
     CDS             complement(22742..23785)
                     /locus_tag="ACG8NF_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0471292.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24162..25127)
                     /locus_tag="ACG8NF_000787"
     CDS             complement(24162..25127)
                     /locus_tag="ACG8NF_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0471293.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25361..26206
                     /locus_tag="ACG8NF_000788"
     CDS             25361..26206
                     /locus_tag="ACG8NF_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0471294.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26397..27584
                     /locus_tag="ACG8NF_000789"
     CDS             26397..27584
                     /locus_tag="ACG8NF_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0471295.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27590..27946
                     /locus_tag="ACG8NF_000790"
     CDS             27590..27946
                     /locus_tag="ACG8NF_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0471296.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27948..28286
                     /locus_tag="ACG8NF_000791"
     CDS             27948..28286
                     /locus_tag="ACG8NF_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0471297.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28362..28618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28681..29715
                     /locus_tag="ACG8NF_000792"
     CDS             28681..29715
                     /locus_tag="ACG8NF_000792"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471298.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29708..30394
                     /locus_tag="ACG8NF_000793"
     CDS             29708..30394
                     /locus_tag="ACG8NF_000793"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0471299.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30418..31266
                     /locus_tag="ACG8NF_000794"
     CDS             30418..31266
                     /locus_tag="ACG8NF_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0471300.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31463..32236
                     /gene="sufC"
                     /locus_tag="ACG8NF_000795"
     CDS             31463..32236
                     /gene="sufC"
                     /locus_tag="ACG8NF_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0471301.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32249..33535
                     /gene="sufD"
                     /locus_tag="ACG8NF_000796"
     CDS             32249..33535
                     /gene="sufD"
                     /locus_tag="ACG8NF_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0471302.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33532..34767
                     /locus_tag="ACG8NF_000797"
     CDS             33532..34767
                     /locus_tag="ACG8NF_000797"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0471303.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34754..35224
                     /gene="sufU"
                     /locus_tag="ACG8NF_000798"
     CDS             34754..35224
                     /gene="sufU"
                     /locus_tag="ACG8NF_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0471304.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35229..36620
                     /gene="sufB"
                     /locus_tag="ACG8NF_000799"
     CDS             35229..36620
                     /gene="sufB"
                     /locus_tag="ACG8NF_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0471305.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        11975 a         6778 c         7861 g        10037 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1081 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1201 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1261 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1381 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1441 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1501 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1561 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1621 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1741 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1801 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1861 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     1981 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2041 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2101 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2161 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2341 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2521 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2581 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2641 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3181 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3241 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3301 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3361 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3421 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3481 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3541 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3601 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3661 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3721 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3781 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3841 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3901 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     3961 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4021 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4081 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4141 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4201 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4261 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4321 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4381 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4441 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4501 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4561 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4621 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4681 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4741 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4801 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4861 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4921 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     4981 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5041 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5101 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5161 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5221 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5281 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5341 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5401 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5461 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5521 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5581 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5641 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5701 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5761 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     5821 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     5881 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     5941 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6001 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6061 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6121 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6181 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6241 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6301 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6361 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6421 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6481 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6541 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6601 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6661 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6721 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6781 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     6841 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     6901 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     6961 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7021 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7081 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7141 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7201 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7261 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7321 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7381 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7441 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7501 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7561 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7621 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7681 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7741 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7801 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     7861 aaggagctgc gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc
     7921 tagacaaaaa tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca
     7981 ataatttgga agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc
     8041 ccatgcatcc acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc
     8101 cttcaagcat acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa
     8161 tcattgcaga cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag
     8221 aaggggataa tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc
     8281 taatcactac agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa
     8341 aggggtttga cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg
     8401 tcaaaaaagc gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa
     8461 tcggaaattt gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt
     8521 tccatacaga tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa
     8581 tcgatttatt aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat
     8641 ataaacgaga cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc
     8701 ggcgtgcagg tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc
     8761 tgtcacctga agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc
     8821 ttgatacatt agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac
     8881 tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc
     8941 ttgatcttaa tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc
     9001 cttcacacgt attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc
     9061 gtatcagctt tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag
     9121 ttcgtgctgt caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa
     9181 cgccttcatt ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta
     9241 taataaagag aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg
     9301 gtactgggcg caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc
     9361 gataatggat ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc
     9421 acgccgcaaa gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca
     9481 ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca
     9541 aaacagaaaa tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg
     9601 ttattcgaaa aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct
     9661 attgttagat gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa
     9721 tttaacacat gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt
     9781 taaattgaaa cgattgttat ttagggaaaa aggtattttt tgaaaaaaga atcccgtata
     9841 atgattgttg tgcaaacgct atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat
     9901 gaacagtcgg tcgttttaac gaattggaag agaaattatt tgttcttttt atcgggacag
     9961 tttttgtcag gaatcactag tatggtcgtc caatatgcga tcatctggta tttgacaaga
    10021 gaaaccggtt cggcgacaat tttgagtttt gctactttac taggaatgat tcccatggtt
    10081 ttactaagcc catttgtagg accgctagtg gatcgctggg ataaaaaagc tcttttgatt
    10141 gtcacggata ttattgtagc gattttcgcg cttattttag cagtagtcgg aacgatttca
    10201 gaatccttcc cgatttggct agtttttgtg tctttgttta tgcgttcggt cgcacaaaca
    10261 ttccaaatgc caacgatcca gtcgattatg ccgacaatcg ttccatcttc tcacataaca
    10321 aggacgaatg gacagttagg aatggtccaa tcagcgaatt tcatcattgc gccagctcta
    10381 ggtgcggctt tgttttcagt cgttcctgtc aattacctta ttttattaga tgtattgggg
    10441 gcagtttttg gtgtcggctt attgattttt gtgaagatcc caaaagtttc tcctgaaata
    10501 ttggaagttc cgcttactat ttttaaagat gcaaaatttg ggctgcagca attgatggat
    10561 aataaagggt tatggtacat aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca
    10621 gcaatcagtc tgtatccatt gatgacgcta gattactttg gtggttccgt tggacaggcg
    10681 ggagcagtgg aagtagtcta cgcagtcgga atgctcttag gcggtgcact gatcagtttt
    10741 ataggaacat ggaaagaccg aatgaaacct attattatag cctacatcat tatgggcctg
    10801 acgatcggtg caagcggatt ggttccaaac gatagtcaag ggttcttgta ctttcttatc
    10861 ttgaatgcag gagcaggttg tgcaacgcca tatttcaata cactgctgat ggcaatgatc
    10921 cagcagagtt atgaatcgaa tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg
    10981 aacctggcag gtccaattgg attattattt gcaggaccat tagctgatcg tttaggggta
    11041 gaaaaaatgt tcttgttttc cggaatcggg attttattat gcggaatcgt tttattttta
    11101 acgtcagctg cccgaaatta tgataaagaa ttacagaaaa aactggtaaa agaacaccat
    11161 gagcaaaaag acgaatagta tggaatggag ttgtgacaga agtcttgtta caactccatt
    11221 tctgatccta atgttcctgt ggcaataagc ctactcaaat gaaaaatatg agtaattatc
    11281 tttatttgtt taatacttga tatagggcag ttttttcacg acctatcgta cttcctcttt
    11341 gcttcataga tttctttgat ttgttgctca gataacggag tgccaaatgg tagtttttcc
    11401 tttaagagtt ctctttccgg tgcgtctacg cctacattga catcgttctc gataggtacg
    11461 ctgtaatgat ggatatgtcc atgaagattg cggatagtgt cgttaggccc gagcaacatc
    11521 ggataatgag tcaagtaata ttgttgatgg tcgaatttaa cgatggcacc tacatcataa
    11581 aaataaaatt tgggacttcc gtcagtaagc ctaggatcat ttttttctaa ataacggaaa
    11641 aaagcgcgac tgtcatggtt ccctttgatc aagtggattg ttccttgcag ttgagtgacc
    11701 agttccaaaa tttctgcatt tgctttttca tattgcggat gcatagcaag gtcgcctaga
    11761 tgataaacat ggtcggtttc ttttattact gcattccagc tagtgatcaa ctgttggttc
    11821 atttcatcca catttgcaaa gagccgcgga gcaaagtcgt tttttcctaa taagcgttca
    11881 tggaaaaagt gcatatcact gataaagtaa ttcatgtatc ttttgcctcc cttttttctt
    11941 agagtaaaca aaatcactta tctgagcaac tcataacatc taaaaagaaa aggttgtaaa
    12001 tgagacgtgc agctttaagc gataagaagc aattttgaaa aatagttttt cacaatttat
    12061 aaaattattc cttattgccg aaaggctggc cctttttatg agtatgccaa ttttttatat
    12121 cgaatgctca aaagatgagt gattttttgg ttgactatca tgtttgtgta ctgaataggt
    12181 ccagtagaac agtattttta cgacttgata attaagggat gaaattttca gaaatatgac
    12241 gtataatgta aaatactgaa aagttgcgac cttcaactcg tagatgtttc agaatctaat
    12301 tgaaatgatg gtgatatcat gacagacaac agcaaaacac gcgttgtcgt agggatgagc
    12361 ggcggtgtcg actcatctgt aacagcactt ttgttaaagg aacaaggata cgacgtgatc
    12421 ggaatattca tgaaaaattg ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa
    12481 gattataagg atgtagcgaa agtagctgct caaatcggga ttccttatta ctctgtgaat
    12541 tttgaaaaag aatattggga tcgtgtattt gaatatttcc tagccgaata ccgcgcagga
    12601 cgtacaccta acccagacgt gatgtgcaac aaagaaatca aattcaaagc attcttggat
    12661 tatgcaatgg atcttggcgc cgattatgta gctactggac attatgcgca agtcactcgc
    12721 gatgaaaacg gcgtggtcca catgcttcgt ggagtagaca ataataaaga tcaaacttac
    12781 ttccttagcc aattatcaca agaacagctt tcaaaaacga tgttccctct aggcggcatg
    12841 gaaaaatcag aagtacgggc aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa
    12901 gattcaacgg gaatctgctt tattggtgaa aagaacttca aacaattttt gagcaactat
    12961 ctgccagcaa aaaaaggaaa tatggtcact cttgatggtg aagtcaaagg acaacatgct
    13021 ggcttgatgt attatacgat tggtcaacgt caaggactag ggatcggtgg cggaggagat
    13081 tcgcaagaac cctggtttgt tgtaggaaaa gatctagcga caaacacatt atatgtgggg
    13141 caagggttcc atcatcctgc attgtatgcc acaagcttgg atgcaagtga aatccacttt
    13201 acgacgaatg aaccaatgcc aaaagaattc aagtgtacag cgaaattccg ttatcgtcag
    13261 caagacgttc ctgtgacagt tcgcttattg gatgataatc gtgcagaagt cgtattcgac
    13321 gaacctgtac gtgcaatcac tcctggacaa gcagttgtat tttatgatgg aatggaatgt
    13381 ctgggtggtg gtttgatcga ccacgcatat caagaaacaa aagttttaca atacgtttaa
    13441 gcgatattaa atgaatcaaa acagctatgg ctaacaaaag aaacaccttt tcttttggat
    13501 agcatagctg tttttttgca ataaaattaa tttttattca ttttatactt gaaatacttg
    13561 tgtaaaaagt tcataatatc caataatatg aataaagttg ctttttgtta agcaagagca
    13621 gttgcaaaat agaagggaat cttttgcaag tgatttgttc aattttataa gtgagggtga
    13681 tggaaatgga aaagaaatcg acgggtatta gcaaatgggg actggttgct ctggttgtca
    13741 gttcgtcaat cggaagcggt gtgtttggga tcacaagtga tctggctagt tctgcagctc
    13801 cagggccagc gatcctttca tggataatcg ttgggattgg tattttagct ttggtattat
    13861 cgctgaatca tttaggagaa aagcgtccag atctagatgg tggtattttc ggttacgcgg
    13921 aagcttcatt tggtaaatta ggtggcttca tcagtggttg gggttactgg ttgtctgcgt
    13981 ggctgggaaa tgttgctttt gcaacgatgc tgatgagcgc catgggagaa tttttcccga
    14041 tattcaaagg tggacaaaat gttccttcta ttttatttgc aagtattttt atttggggtc
    14101 tgactttatt agtaaacaat ggagttgaag gagcgagcgt catcaataca atcgtgacga
    14161 tctgtaaatt agtgccatta ttcctatttt taatatttgt atttatcgca tttaaagtga
    14221 atatattcac tgctgatttt tggggtaacg tatctgataa tctagtgaat ggaacagggc
    14281 agcaaggaac gatctggttg cagattaaag gttgtttgat ggttatgatg tgggtctttg
    14341 tagggatcga aggcgcaagt gttttagcca accgcgcgaa gaaacgttca gaagcccagc
    14401 aagcatcgat cattggtctg ctttgtttat tgtttatcta tatccttgct tcgatcttgc
    14461 catacggcgt cttatctcaa gaagagttag caactatttc acaacctgct atggcaaata
    14521 ttttaaaagg aatcgtggga aattggggag cagcgctgat caatatcggc ttgattattt
    14581 cgatcattgg ttcttggttg tcttggacga tgctcccagc agaaacaacg atgttgatgg
    14641 ctcgagacgg tctgttgccg aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt
    14701 attcgttagt tgttactgca atcttgacta atctattctt attgactttc ttagttactg
    14761 attatgccta tcaatttgcc tattctttat gtacagcggc aattttgatt tgttatctac
    14821 ttgtcgggat ctatcaagtc cagttttctt taaaagaagg aatcaaaaaa caagtagtga
    14881 tcggtgggat tgctgtagtt tttgaattga tgggcatcat tttagcagga ttttcttatg
    14941 tccttttatg tagtattgcc tatcttcctg gtttttgctt ctactggatc gcttgtcgtg
    15001 aaaccaaaca tcaaattacg ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg
    15061 cactgctttc aatcgggttg cttgctgtag gctggatcaa gatataaggg ggaaagaaac
    15121 atgaaaatcg caaatttcgg tgtagaagaa tggctaaata aatgggaaaa gaaagcggta
    15181 tatgatattg cacaaagctc gatcgaagca ttgacattag acgaactcgt tggcttagat
    15241 gggactagcg taagtaaatt tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg
    15301 atcgaaggat cagatgtttt caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg
    15361 gaaaacatct tacaaacgaa tggagcgact ggtgctaatc ttttagctct atacgcgtta
    15421 gtggaagcag gagaccatgt tgtttcgatg cttcctactt atcagcaatt atatgacatc
    15481 cccaaatctt taggtgccgc tgttgacttt gttcatttga aggaagaaga agattggcag
    15541 tttgatttag aacagctaga agccttagtc aaacctgaaa caaaaatcat ctgtttgaat
    15601 agtgcaaaca atcctacagg cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa
    15661 attgctcgta cagtagatgc atatgtattg atagatgaag tatatgcgcc tttaacggat
    15721 aaaggtgaat tcttgtcgat cgtagatgtc tatgaaaaag gtatagcaac caattcccta
    15781 tccaaaacct actccattcc aggtattcgt attggctgga cagctactgg acctgagttg
    15841 gcagaagttt ttcgaaagta tcgagactac acgatgattt gcggcggagt cctgtcagat
    15901 gatcttgctg tacatgcatt gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc
    15961 attacagaga accttgtgat tttaaaacaa tgggtagcca atgaaccgaa agttgaactt
    16021 gtcgctccca attacgtctc tacttctttc attaaattag ccatcgaaga agatgaccag
    16081 acattttgta tcaatttatt agaagaaaca ggagtactat tagtaccagg aagtgccttt
    16141 gatttaccga agcatgcccg actaggttac tgttgtaaaa aagaaacctt ggaaaaggga
    16201 ttattcctct tatctgagtt cttgaaaaaa caggcctaaa caaaaacact gcaaggacag
    16261 tctgtgacaa ctgttcaagc agtgtttttc atttacgctt attgtctatg agaaggagag
    16321 tgccctgaag ctatggagac tcctgaagct ttttcttctg cagtttcttg ctgatggatc
    16381 tcttgtgtat tgtgtcgtac aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga
    16441 aagacgccgc gataaccaaa tgcactggaa acactggagc cgatcatagg ccctactacg
    16501 ttaccagttg cttggaaaga ttggttataa ctgaagatcc gtccagcagc ttgatgaggc
    16561 gaatattttg tgatcaatgc ttggacagca gggagcagac aagcatcaga gataccaatc
    16621 aaaaaacgta aggcagctaa ttgccaaaca ttcgtgacga atgccattgg gatataaacc
    16681 agaatcgcaa aacccaaacc aatcgcaagt attctttcac tcccaatctt gtcacctaag
    16741 cgaccaaatc ttggagcggc tatcaaagtg gcaatcccag gtatcgaagc aatgattccg
    16801 ctgatcaacg taacattccc atgtccatgc agcaattggc gaatgtacag actgatgatt
    16861 ggactaatgg aattgttgga tgcttggatg atcatcgttg tgacgaacat accgatgacg
    16921 acgtgtggat atttcaagtc tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc
    16981 actgggacaa atttttcatg gacgaaaaac agactcaaca agaagacgaa aaataagatg
    17041 atccctgtaa tgaaaaaggt agggcgatag ccaaacgctg aagcagagat cccgccaaga
    17101 agcggtccaa gtaatgttcc tgtaacagaa ccagttgcta aggttccaag aacttgacca
    17161 cttttttctt ttggcgtccc agttgcgaca agggcagtgg cattactgat ataaccagaa
    17221 aaaatccctt gaagcagacg caatgcgact aattgataca cactagtcac gcaacccatc
    17281 aaagaaatga cgattgccat tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt
    17341 tggtcggcta attttcccca ccaaggagag ataagcgtag tcaccaagaa agtagatgaa
    17401 aaagtcaatc cgctccaaaa gttcaattgt gacgtactat aattacctaa cgtatctatg
    17461 taaagtgaca taaaaggcat gactaaacta aatccgatac cagccataaa tgttccaaac
    17521 cataagacaa acaagttttg ttcccaagtt tctcttttcc taaataattt ttcctttatt
    17581 cttgcagcca attagactcc ttctttctga aaattttcat ctacagctct ttattataaa
    17641 ctacaatatg tagtttatca atgaaagtct cttttttctc tttctttttc gtggtaaact
    17701 gaaagagtga aggatttatt ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc
    17761 tcaagagaaa atcatcgact gctttcgaga actagcagaa gagatggatg ttcagcaaat
    17821 aacctttcag catttagcca aacgcttgga tatcaaggct ccctcgctgt ataaccattt
    17881 caaaaatatt cgtgatgtca aaacagcgct aacagcgaaa cttctgcagg aacttaatga
    17941 ccagctgcga cgggctcttg tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga
    18001 aacttatcga tcctttgctt tttcaaatca agcagtgtat gagttactga tcagtgtacc
    18061 gcataccaat gaagctgttt tattagatgg aatccatgaa acgaatcaga tcattttgca
    18121 gatatttgag gcttttcaat taacgaaaga agaaaaagtt catcgaagca gggaacttcg
    18181 tagtatgatc cacggttatc tttccttacg ttttttagga tactttacaa aagaaccaac
    18241 aatcgatcca gaagacagct atttttggat gatcaataac tttatcgcta ctttgcctta
    18301 aacaatcaga ttcatttgta acaattttaa gatgttcatt aaatgaccct tttataagag
    18361 aaaattcttg caactttctt gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg
    18421 agcacgagta tgtatcaagt aattaccatg ttcggggaca atgagccttg gtggtttttt
    18481 gaagactggg aagaagatat tgaagaagaa gaaacattcg agtctttcga agaagcacga
    18541 caagcgtacg aaaaacaatg gaacgaaatc catcaaaatt atgaatatat caatgcaaaa
    18601 agtaactttc tcagcgcctt ttggaatgac ggagatgagc gctggtgtga agagtgtgac
    18661 gatgatttac agcaatacaa gggacttgcg ttgttgaaaa atcatcagcc catcacagta
    18721 gaaagcagaa aggaatttta tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa
    18781 agacctaagc aaggtgcttg gtgctaatca aaagcacaaa aattattatg aaggacccaa
    18841 ggtgatcgtt acttgggccc ttggtcattt attaggattg aaaatgccgg aagatctgaa
    18901 taaggaatgg cagagctggc aaatggacac gttgccaatg atccctaaaa aactaggaat
    18961 caaaccactt cctaaaacag gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa
    19021 agatgtctca gaagcagtca ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg
    19081 ctggatctta gagtatgtgc atttcaataa accagtgaag cggttatgga tctcttctca
    19141 aacagataaa gcaatcaaaa ctggattcaa gcaactaaga cctgcgaaag aatatgatgc
    19201 attgtatgaa tctgctcttg cccgggcaaa agcggactgg ctggtaggat tgaatgtgac
    19261 cagagcctta actgtgaagt acgcggataa tttatcagca ggccgtgtgc agacaccgac
    19321 actagcatta gtgagagacc aagaaaagaa aatcgagcag ttccgtccac aaacgtattt
    19381 cacgatcacg ttgcaagctg gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc
    19441 tttaaaaaca caagaagaag cacaaaaact tgttcaattg atcagtacaa gcaaaggaac
    19501 tgtcacagaa gtccaagaaa aattaaaaac ggaatctgcg ccattgcctt atgatttaac
    19561 agagatccaa agagaagcca atcaacgcta tggctattct gcaaagaaaa cattaagtct
    19621 tgtacaaagt ttgtatgaga cccataagat cgttacttat ccgcgtacag acagcaaata
    19681 tctgacaaat gacatgaaaa gtacgatgaa agaacgattg caagcagtca gcgactttgc
    19741 tcctgaggtc aagcagtatt tgaaaaacgg agccatcgtc caacagacaa aagtattcca
    19801 agacagtaaa gtgaccgatc accatgcact tttgccaact gaaaacagag cacgttatga
    19861 aaaattaagt aatgaagagc gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact
    19921 gtttgctcaa ccccataaag tcagccaaac aaaagttacg gttgaatttg gtaaagaacg
    19981 attcattttt cggcaaaatc gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga
    20041 gacggaaaca gtcaaatggg aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa
    20101 agaactatct gcaccgccta aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa
    20161 atacagtctg ggaaccccag ctacgcgtgc agagattatc gagaaactaa tcaaatctga
    20221 actgatggaa cgtacccctc aaggactgca agtatctcct aaaggtaagc aattattagt
    20281 attggtaaat ccctctttag taacaccaga tttgacagaa agctgggaaa aagaccttga
    20341 agctattgct gcaggaaaga aacaagcagg aaccttcttg aatggaatcg aaaaagaaac
    20401 aaaacgcttg gtgaatgaga tcaaatcaag caaacaagaa tatcaagatt tctcgattac
    20461 ccaaaagaaa tgcccagaat gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa
    20521 aatctatgtc tgcaccaatt cagattgcaa ctaccgcaga agaaaagatc caaaagtctc
    20581 taatcatcga tgcccgcaat gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc
    20641 ttatttccgt tgtaaatacg atggaacgac agaaaaattg cttgataaaa aagagcagaa
    20701 gaagaaaatg accaaacatg aagaacgacg cttgctgaaa aaatatgcac aagcagaaga
    20761 accagaagaa agcccattag cagcagcact aaaagcagca atgaagaagt aataagatat
    20821 gaaagaggtt gtgatagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg
    20881 cttctcaaat ttttgtgaaa tttcggctta tttccgaaga agctacttct ggaacaccat
    20941 ttattcggaa aaaaggactg tgacaagact tttgtcacag tcctttttca tttaaaaaaa
    21001 tgtttacttc atttgacgta tattattggt gaggcttcaa ttcatttggc ttcacttttt
    21061 ctgtttctct tatgatataa acaggacgct gtttcgtttc taagaagatt ttcccaatat
    21121 atttcccgat gattccaaga cataacaatt ggattccacc aatgaacagg aagatcgaga
    21181 ccatggaagg ccatcctgat gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca
    21241 aaattgcaat accagaaccg acacaagaca aagcgccgat aaatgaagca atcgttaacg
    21301 gtgcatccga gaagttcacg ataccatcaa tcgagtagtt gaataatttc cagaaggacc
    21361 aagatgtttc gccagccaca cgttcgcggt tttcataaga aatatattct gttttaaatc
    21421 cgacccagct aaataatcct tttgaaaatc ggttgtattc tgttaattct aggacggcat
    21481 ccaccatttg acgagtcatc aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc
    21541 tgattttatt gatcaactgg tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc
    21601 cttcacgagt cgaacgccgg gtgccgacac aatcgatatc agaagtagtg atccggttga
    21661 tcatctcagg aagcagttcg ggcggatctt gcagatcaac atccatcacg gtcactaaat
    21721 ctcctgtaga agcttgcaat ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa
    21781 atgagacaaa gcggacataa ggataagctg aggctagctt cttgattgct gctaacgtac
    21841 cgtcttttga tccgtcgtca ataaacaaat attcgaactc atgggcacct ttgaattttt
    21901 caacctcatc aaaaaaaaga ggaacagttt tttcttcatt aaaacatggg acaacaattg
    21961 atactttcat cgtttctatc ctttcaatcg tatgtttata tatggtttac attcaaaatt
    22021 taataaacct taaataacac ctttaaaatt tgattttacg taaaagaaca ggtattcttt
    22081 ttgtaaacta acgtatttag tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa
    22141 tttttattag ttggcaagta gttttggata gagaaaaagg aggaagttcc agtctattga
    22201 ctaggagcaa acaaaatgaa aatacacaca cctatcggct ttttatggtt agatgcagat
    22261 gaatccggat tgactgctgt atcttttcag ccaatttcag aaagcgaatc agcgaatcag
    22321 cgaattttaa cgcgcacaca agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa
    22381 tttgatgttc ctttatctat aaagagaggt acgccttttc agcaaaatgt ctggaaagct
    22441 ttgtcgacca ttccttatgg agaaatccga acgtataaag aagtagccgt tgctgttgat
    22501 agtccgaaag ctgtgcgagc gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt
    22561 attccttgtc atcgagtgat cgggcaaaat ggaaaactga caggatatat gggaaacgca
    22621 atagatgggg ttgaaatcaa acgccaattg ttgttgttag aaggatatct gcaaaattaa
    22681 aaacagttgt gattcggaca ttgttccatg cgagaatcac aactgttttt tcatgttcat
    22741 tctaaacttt gatcagccaa ataaatagta aagccatcac tgtttggaga agcccgacag
    22801 ctaatccata agataagaag cgtgtacctt ctttcatgaa ttttgcaaag tctaaacgca
    22861 gaccaattgc cgctaaagca gtgatctcaa accagctgct gataaaatgt gccgattggt
    22921 caataactac aggtaaagaa aacaagctgt tgaccaaaca aaagatcaca aaccctaata
    22981 cataccaagg aagcggtaat tttcgttttt tctgcgtatc agttggcgtt tcaaacagag
    23041 ctggttgttt cttgtgcatg aatttttcaa acagatagac aacaacaacg agcatcataa
    23101 tacgtagaat cttaaatagc atggcgaatt cgacaacaga attgtcaagt aagcttgccc
    23161 cagcgactac ttgaccaaca gattggagtg taccacccaa aagtgcactt ttttcaatga
    23221 gatttccacc aaacaactgt aatccaagaa aagggagagt caacatcatc acagtaccca
    23281 gtagattgac tagtgtgatg atctgtcctt tttcttcctc tttcgcatgg atggaagggg
    23341 cgattgaagc aatagcagaa gaaccacaaa cagcgtttcc tccagccatc atcaaagaag
    23401 aagtatcgct gaattgcagt tttttaccaa tcaagtaagc accgatgata gtgactgaca
    23461 tttgcagtaa cacaaaaaca attcctttta caccaatttg agcaatcgtt tgagctgtga
    23521 cagttgcacc tagtaaaaaa acagagaatt ctaatagttt gctttcagca aattttgtgc
    23581 cgattgctag aacaggctgt ttaaaaaaag tatttcctaa taaaataccc aataaaatag
    23641 caatagtcgc tccacctaac tgtggcagat aaatagcaag aagcctgctg aaggcagcga
    23701 tggcaaaaga aagcagtaaa ccgggtaaga tttcgcggac cgctgaatcc tttacagatt
    23761 ttattggatg tgacaatgat ttcatttgat cggttccttt cgtcaataaa tactaaaagc
    23821 atactcgaac atagtagcac taaaccagta ttttgacaaa agaacaagag gtcgtgacaa
    23881 aagtcttgta cgacctcttg tttcgaataa acggtgttcc agaagtagct tctacggaaa
    23941 taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    24001 aagctgacca cttcttttac gacctcttgg taaacggtgt tcaaaagctt gcatctgcgg
    24061 taataagccc aaccagtaca aaaacatgaa cagctgtttt tgtttggccg ttcttattac
    24121 ttgatgcaga acagcttttt cacaacctat tgaatcgatt attaagcgat ttttttagaa
    24181 acaaattttc catgtttgct atgttcttta attaagaaac gtacgtaatg agcaagaacg
    24241 tagaagacaa ctacacttgc aaacagacat tccgcaactt ttaagaaatc caatacgcta
    24301 ctatagatac ctaaactgtt gaagtaatga acagtagtaa atatagctgt tgcagagata
    24361 acaagcggga atgtaaaggc tgcatagctt ggataaaaac gaagcttgaa caaacgtgga
    24421 agcatagaca gcacaacgaa atacaatact tgagataaag cgaacaatgc tacgacaaga
    24481 taaaaattag gttcagcaaa gtttttaaga taacctgtta ggcaaagaga accaggtgct
    24541 gcaacgatcg taatcagcgg acgtgtcgga tgagccatat ttcttacgaa aaagacacgt
    24601 ttgatgatga tcggcaataa gaacgcatag cagatcaatg aaatccaaaa gaaaattttt
    24661 ccaataagtg ggaagaagtt cccggcagtt acagtgatga ccccaaatcc aacgtaaatg
    24721 ataaaccagc ttggataaat tgcttcaatt gaaagatcag ctttgataac atggtgataa
    24781 ttgaaataaa agacaaggat cacttggaag ataacagcgc acagccaaat gaatttgaca
    24841 attggtgcaa tactttcata tgaaacaaaa taagtacaaa taaccatgag tgacatcgta
    24901 aaagttggtg aaacggaggc gatgattgga tcatgcaacg tttgtttcgt gtgttcgaat
    24961 aagataaaca atttaacaaa aatgaatatc ataagaatca tagagatacc gccagttaga
    25021 ttgcctaaag caacatggtg atagtcttta aacagatttc caagggaagc taacccaagg
    25081 atcaacccgc agatcgggat cggaataatt ttcaaaaagt tcttcatata taatattctc
    25141 ctttttattt aatagatttt agtaccaaga tgaacgaata cattgtaaaa tgaataggaa
    25201 tgtatgatta cgagcaagca gacagctgat tgtcttcatc taagttacta gttggatcag
    25261 caactgtttt gttgtacatt tctattatag atggtttttt gtatatttaa atcaaatagt
    25321 ttttatagta attatcataa aaagttatgg aagtggtgcc atgtttaagt tattaacgac
    25381 ttttcgagca gtatatgaaa caagaaattt ttccagagca gcagaagtac tgtttttatc
    25441 tcaaccagca gtctcgaatc aaatcaaaca gttggagcgg gaattgaata tcgatctttt
    25501 tcaacgtaac ggaagaaaag agatgcttcc aactaagcaa gccgacttat tatatgaacg
    25561 gacactggtt ttgttagaag aatgggaaga aactcagcaa aaattgctca atgaacaaaa
    25621 tgaagaagaa gtatgtcgga tcgttgcttc ccacaccttt gctgtctatc ttttgcctca
    25681 gttgatgaaa catctgatca aaaggtttcc taaagtgcat tttgagatcg aaatcacgaa
    25741 ttctcatgaa tcattagaac aagtagcgaa acatgaatcc gattttggtt ttatcgaaaa
    25801 gccattagag acttccggcg tacaaagata ctctttgatg gaagaccagc tagtcatcgc
    25861 tggggaccca aaaagcaaat tgtggcttgt aagagaagca aactctggtg tttttcatta
    25921 cacgcagcgt tattttgaag agatgaatat ccaagataag aagatgatcg tgaaaagtaa
    25981 cgatgtaatt gttgcgttgc tgaaggaagg aatcggtcgc tcgattttgt ctaagcgctc
    26041 tgttccagaa aatgtgccaa tgactccgtt gaatttccat cgatattttt atttcatcca
    26101 acgaaccaat ctggtttctg atcagctgct agagattgcc gaagagatcc ttcgatacaa
    26161 tgaaagcaca gaaacttctc acagcgagga gaatctcagc tggtagataa ttgcatgtat
    26221 caaaaaaagt tctatgatac actaaagata agcagtaggc ttttgtactg catttgctca
    26281 agcaactgca caaacggaaa gttaattttt cccaaatgtc tatggaaaga ctttccatta
    26341 tctaactgga ccgctataat aatcagcagt atggttttta agaaaaggga tgaaacatgg
    26401 aaaaaaaatt aaaagcttcg aacgataatc gtattgatta tggcgtgatt ttaccagtct
    26461 ttctgttatg tttgattggt ctgctctcat tatatgtagc acttagccat gatccgaaca
    26521 atccagcagt cttgaaaggc gtagggatgc aagttgtctg gtatttagtt ggagcggcag
    26581 caatcgttgt catcatgcat ttgaattcca aatggatctg gaaactgact ccttaccttt
    26641 atggtttggg gttagtggta atgggactct tgctcaaatt ctatgatcag aatttagctg
    26701 cttcaacagg gtctaaaaac tggtttcgat tcggtgcgtt tactttccag ccagcagagt
    26761 tgatgaagat tgcgtacatc ttgatgatgg cactgatcgt aactaaacat aatacgcaag
    26821 taaaagaacg gacgatgaaa tcggactttt ggctgattgg gaagctcttg atcgtgacaa
    26881 taccggtttt agcattgatc atggcccaag atgactttgg aacgatgctt gtctttcttg
    26941 cgatctttgg cgggattttc ctgatgtctg gaatctcttg gcgcatcatc gcacctgtag
    27001 taatcttggc agtagtcgtt ggagcaggaa cgatcttctt ggttacgact gaaggcggaa
    27061 gagacttgct ttataaagtt ggattcaaat cttatcagtt tgcacgtatt gattcatggc
    27121 tggacccttt ccatgatacg tcaggaatga gttatcagcc ggcccaagga cttttggcaa
    27181 ttggtaccgg tggcttgttt ggtaaaggat tcaacgtaag caatatctat gtgcctgttc
    27241 gtgagtccga tatgattttt accgttatcg gtgaaaactt cggctttatc ggtggtgcat
    27301 tcgtcatttt cttgtatttt attttaatct atcgtatgat tcgggtgtgt ttcgatacga
    27361 ataatgaatt ttacgcctat atcgcaagcg gtttgatcat gatgctgctg ttccatgtct
    27421 ttgagaatat tggagcaaat atcggattac ttcctttaac agggatccca ttgcctttca
    27481 ttagtcaggg aggatcctcg attttaggga acatgatcgg tatcgggttg atattatcga
    27541 tgagatacca aaatgaagca cctgcaagat cttcaaggag gtaagaaaat tgtacacctt
    27601 ttactggtac ccgaaatgtt caacatgcaa aaaagccaaa gcgtggttag accagcatgg
    27661 cgttagttat caaaccgttg atatgatcaa agaagtaccg aaggcctcaa cgattgagac
    27721 ttggctagaa acgagtcaag taccaatccg tcgcctgttc aacacaagcg ggatgaaata
    27781 tcgggaactt ggactgaaag atcaagtagg cgagtttacg gcttcaaaag cgagccaagt
    27841 cttatcgaca gatggtatgc tgatcaagcg accactgatc ttacgagagg accagcttgt
    27901 tgcaattgga ttcaatgaaa aaatttatga aggagaactg atataaaatg gcaggcaagc
    27961 atttgaagaa aaaggacaac ctttggatct tatttaatgg aaaagaatac tgtgttggtt
    28021 tgacaaatga agcgcaagaa gaacttggag acatcacgtt tgccaatctg ccaaaagcag
    28081 gacaatcgct aaaaaaagat gaacctctga tcgaagttga agcggaaaaa gcggtcaatg
    28141 aatttgccag cccgctaacg ggtacagttt cttctgttaa tgataaaatc ggtgaagata
    28201 tcaatatctt gaatgattca gatgaaatga atgcgtggat cttaagtttc aaagatgttg
    28261 atccaaaaga atttgacgaa ttataaaatc agaaaatcac ttgaaaaaag ttgacactga
    28321 ttcttccagt tgctataatc ataagcaatc ctatatgaaa cgagaaagct ttgaaaagaa
    28381 gagtacatgt cacggacgtt tgaagagagc cctgtttgct gaaaatgggt aacgaaaatg
    28441 agatggaaga tggtcttaga gcagagtaag cgagcgtaag caaacttact acgggagtgc
    28501 ccgttacagc gcgacagtat acggaaagaa gctaagtcca agtactggaa gaggctgtta
    28561 ttgcgaagta acggtaaatc aaggtggtaa cacggaagtt caagctttcg tccttttgct
    28621 taaggcaaga gggcgaaggc ttttttgttt gaaaaattat aagagaaaga tgtgaatggc
    28681 atgtcattaa tccaactaac gaatgtcaaa aagacattca atgggaaaaa cggaaacatc
    28741 caagccgtca atgacgtttc actaaacgtt gaaaaagggg atatttatgg aatcgtcggc
    28801 tattctggcg caggaaaaag tacattagtc cgtctcctga atggacttga gcttcccacg
    28861 agcggcgaag tgatcgtaaa cgatcaagat attacaaaat taaaaaacaa agaattacgt
    28921 acattccgaa agaaaatcgg tatgatcttc cagcatttca acctgttatg gtcaagaact
    28981 gtacttgaaa atatccagct gccgctggaa ttagctggcg taccaaagag caaacgtaaa
    29041 gaacgggcag aagaactgtt gcgtttagtc gggttagaag gacgcggaca agcatatcct
    29101 tcacaacttt ccggcggaca gaaacaacgc gtcggtatcg cacgagcatt ggcaaatgat
    29161 cctgagatcc tgctttgtga tgaggctact agcgcattgg acccgcaaac aacagaagaa
    29221 gtattagact tattgttagc gatcaataag aaattgaatt tgaccatcgt cttgatcaca
    29281 catgaaatga atgtgatccg caaaatctgc aacaaagtcg ctgtgatgga gttggggaaa
    29341 gtcgtagaag aaggagacgt attgactgtc ttcagacaac cgaaaaaaga agtaaccaaa
    29401 cgctttgtac aacaagacat cgaaccagaa gctgattcga cagaactatt agctgaactc
    29461 atcaaagaaa atccaagcgg ccgtttggca actttgacct ttagcgaaag aaatgccaac
    29521 gagccagtca tttcccaagc gattcgacgc tataacatcg atgtcaacgt tgtgtatgga
    29581 aagatcaaac aagtgaaaga aggttccttt ggttcattga ctgtcttgat gactggtgaa
    29641 accaaagaat tagatcaagc agaagtgttc atcaaagaac aaggagtagg aattgaggtg
    29701 atccatcgtg gataaaagct tttcagaaac atatctagat tttaaacaaa tcaacccgga
    29761 aacgatgaat caggctatcc ttgatacact atatatgaca ttaatctcaa tggtcttagt
    29821 gacgatcatc ggattagttt tgggattgat cctttactct attggacgca aaaacaaacc
    29881 gtatgctcga atcatttatg gaatcgtatc gatcatcagt aatattttcc ggtcaacgcc
    29941 atttatgatc ctgatggttt tgatcattcc atttactaaa gcaatcgtag gcactatgct
    30001 aggtgcgcaa gcagccattc cagctcttgt tttatccgca gcaccatttt atgcccgtct
    30061 agtagagatt gcttttcgtg aagtcagccc tggtgtactg gaagcagcag atgcaatggg
    30121 agcaagttac tgggaaatca tctggaaggt cttgatacct gaaagtattc ccgcattgat
    30181 ttctggatta acagttacaa cgatctcgat gatcggattt actgcgatgg ccggcgcgat
    30241 cggcgcaggt ggacttggag gtcttgcttg gcaggaaggc taccagcgtg gtaatctaac
    30301 agtgacattc gttgcgactt taattatttt actaatcgta tttatcgttc aaggaatcgg
    30361 tgatttctta actaagaaaa cagacaaaag ataataggat atagggggat gtaggatatg
    30421 aaaaagaaaa tttttggatt tgcagcagcg cttttgctga ctgtaggatt agcagcatgc
    30481 ggtaacaaca gcgacagcaa agattcagca aataaagcag acgacacgac attgaaagtc
    30541 ggcgcttcac cgacaccgca cgcagagatt ttggaacatg tgaaaccatt attgaaagat
    30601 gaaggaatcg atcttgaaat cgtcaaattc gatgactatg tattaccgaa ccaatctttg
    30661 gaagaaggcg acattgatgc caattacttc cagcatattc cttatttgaa caaagaaatc
    30721 aaagaaaaag gatatgactt tgtcaatgcc ggtgctgtcc acatcgaacc aatgggcttg
    30781 tactctaaaa aagtcaaaga tgtttccgaa ttaaaagatg gtgcaacaat cattacttca
    30841 aattctgaat cagactgggg acgcatcatc acaattctac aagatgctgg cttagtcaaa
    30901 gtcaaagatg gagtagatct ggaaacagca acattcgatg atattgaaga aaaccctaag
    30961 aacttgaagt tcaaccatac gatcgaccca tcacttttag cttctacgta ccaaaatgat
    31021 gaaggtgacc tagttgcaat caacgcaaac ttcgcttacg gcgcagggtt gaatccatta
    31081 gaagatgccg tattgcttga aaaggacaac tctccttacg tgaatattat tgcgactcgt
    31141 aaaggtgatg aagacagtga caaaatcaaa aaactgattg aagtgcttca ccgcaaagaa
    31201 gtccaagaat ggatcacaga caaatggggc ggctctgtta aaccagtaaa ggctgatgca
    31261 aaataaggta tggaacaaaa aagatgagaa ataagtctgt aatggcttat ttctcatctt
    31321 ttttatacct attctaattt agaaatcaaa attgtttaat aataaataaa agatatttga
    31381 ttttagtaag gaaggtcatt gcgcttcatt ctcaattagt ctaaaatgag tgagaacgaa
    31441 agagaatttg ggggtataat gcatgtctgt cttagaaata aaaaatctac atgtgtcgat
    31501 cgaggaaaaa gaaatcctta aaggcgtaaa cctaacaatg aaaaccggag aaatccatgc
    31561 catcatggga cctaacggca caggaaaatc taccttatct gccgctatca tgggcaatcc
    31621 aaactatgaa gtaacagaag gcgaaatttt gtttgacggg aaaaatgttt tagacttgga
    31681 agtagatgaa cgtgcgcgac ttggcttatt tttagctatg caatatccaa gtgagatacc
    31741 aggtatcacg aatgcggaat tcatgcgtgc agcgatcaat gcgaaacgtg atgaaaacga
    31801 caaaatttct gttatggcat ttttgaaaaa gttagatgaa aaaatggctt tactaaatat
    31861 gccagaagaa atggctgaac gttatctgaa cgaaggtttt tctggcggag aaaagaaacg
    31921 gaatgagatc ctgcaattgt tgatgttaga acctacattt gcaattttag acgaaatcga
    31981 ttcaggtctg gatatcgatg ctttgaaagt cgttgctaaa ggagtaaatg aaatgcgcgg
    32041 agacaatttc ggtgcattga tcatcaccca ctatcaacga ttattgaact atatcacacc
    32101 tgatgtcgtt cacatcatga tggaaggccg agtagtcatg acagggaatg ctgacctagc
    32161 taaacgtctg gaagcagaag gctacgctgg tatcagtaaa gaattaggaa tcgactataa
    32221 agaagaagaa gcgtaagggg gagcaagaat gaaagaaaaa aactggctcg attatcttga
    32281 cgcggtaaat gatttttctt taagtaaagg cgaacctgat tggatgagaa ccttccgtca
    32341 agacgcacta gcgaaagcag atgaactgcc attgccgcat atcgatcggg tgaaattcca
    32401 tcgctggtcg ttatttgatg tgaaagaaac acaaaccatc agtgaaacag gcacgattcc
    32461 ggcatttgat gcaatgaaag acaatcctgt gcttgtccaa caaggttcat ggacgatttt
    32521 tgaacaactg cctgtggaat tagcagaaaa aggagtcatt tttaccgact tgttcactgc
    32581 aatgatcgaa tatccagaac tagtccaaga atattatatg aaaaaagcag taaacatgaa
    32641 cgaagaccaa ttgactgcac tccatgtagc ttttatgaac agcggtattt tcctgtatgt
    32701 gcctaaaaat gtcgtgatcg atgaaccact cgagtcctta ttcattcaag atggcgcatc
    32761 cgatgagcac ttcttcaagc atgtgttgat cgtggcagat gaacatagtg aattttctta
    32821 tctggaacgt ttccagacaa ccaaagaaca agtagcaaaa tcatcaggta atatcatcgt
    32881 ggaagtcatc gctaaagctg gttcaaaaat caaatattca gccgtagatc aattaggaga
    32941 aaatattact tcttatatga atcgccgtgg gcacatttta cgagatgctt ctgtcgactg
    33001 ggcaattggt gtcatgaatg acggtcatgt gatcgctgac tttgactcag atttagcagg
    33061 agaaggtgca cacgcagaag tcaaaatcgt ggcaatcagc tcgggcagac aaatccaagg
    33121 aatcgataca cgagtaacca ataaagcccc tcatacgatc gggcatattt tgcaacacgg
    33181 agtgatccga gaaaaaggaa cattgacctt taatggtatc ggccacatct tgaaaggggc
    33241 aaaaggtgca gatgcccaac aagaaagtcg tgtcttgatg ctttctgata aagcacgtgg
    33301 tgatgcgaat cctattcttt tgatcgatga aaacgaagta actgctggac atgctgccag
    33361 tgtaggacga gttgatccag aagaaatgta ctatctgatg agtagaggat tgcataaaga
    33421 agaagcagag cgcttagtga ttcgaggatt cctcggttct gtcttaacag caatccctgt
    33481 agaacaagtt cggaaagaat tagtcgaagt aatcgaaggg aagttgaatg gatgatcgat
    33541 gctaaaaagc tacgcaatga ttttcctatc ttatttcaag acgtcaatga tgaaccttta
    33601 gtttatctgg ataatgcagc gacgacccaa aagccaaaac aagtactcca ggcattagcg
    33661 tactattacg aacacgataa cgccaatgtt catcgcggtg tccatacgtt agcagaacgc
    33721 gcgacccatg attatgaaca agcaagagaa aaagtacggg cgttcatcca tgcaaaagaa
    33781 acagcagaag tgctgtttac tcgtggtacg acgacaagct tgaattgggt agccagaacc
    33841 ttcggcgacc gctatatcaa agcaggagac gagatagtgc tctcttacat ggaacatcat
    33901 tctaatgtta tcccgtggca gcagttggca aaacgaacag gtgctgtctt gaaatatatc
    33961 gaactaacag aagaaggctt cctggatatg gaggatgcag cgaaaaagat tacggacaaa
    34021 accaaaatcg tctccattgc tcatgtatcc aatgtcttag gtgtcttcaa tcctgttcaa
    34081 gaattagctg aacttgtcca tgaaaacggc ggggtcatcg tagtagatgg tgctcaatct
    34141 gccccgcaca tggccattga tgtccaagca ctagactgtg atttctttgc tttcagcgga
    34201 cacaaaatgt gcggaccaac aggtattggt gttttatatg gtaaacgaaa atggttagaa
    34261 gaaatggaac ctgttgaatt tggtggcgaa atgatcgatt ttgtcgaatt atacgacagt
    34321 acatggaaag aactaccttg gaaattcgaa gcagggacac caaatatcgc cggcgctatc
    34381 gcattaggcc atgcaatcga ttatttgcaa aaactcggta tggacaatat ccatcgatat
    34441 gaagaagaac tagccgctta tgtgttgcca aaattacaag caattgatgg attgactgtt
    34501 tatggtccac aagacccgaa ttttcggaca ggagtcgttg cattcaattt ggatgggtta
    34561 catccccatg atgtcgcgac agctttagac atggaagggg tagccgtcag agcaggtcat
    34621 cattgtgcgc aaccgctatt gaagtacctt gaagtatcag cgactgctcg cgcgagcttt
    34681 tatttctaca acacaacgca agacgccgac cgtttagttg aagcaatcat ggcaacaaag
    34741 gagtttttcc aacatgggac tatctaaatt agacaattta taccgtcaag tgatcttgga
    34801 tcattcttcc catccgcatc atcgtggaag cttgactgac tcgacaagca agatcgaatt
    34861 gaacaacccc acatgtggag atgtgatcga attgcaagtc gatgtaaaag acggaatcat
    34921 caaaaatatc gcctttgacg gcagtggctg ctccatcagt acggcaagtg cctcgatgat
    34981 gacagacgcc gtcatcggaa aatcaataga agaagcagaa catttaactg tagactttct
    35041 acagcttgtc caaggaaaag aagtcaaaga tcccgaccaa ttaggcgatg ctgctatgct
    35101 tggtggtgta gctaaatttc ccgctcggat caaatgtgcg acacttgcct ggaaagcact
    35161 aggaaaagca atggaaaacg atggtaccgc agctttccat cattaccaca aggaagagga
    35221 gtgagaacat gggcgtacca gaattagaag aatataaatt cgggtttcac gatgatgtcg
    35281 aaccggtttt cagtacagga gaaggcttga cagaagaagt gatccgtgag atgtcaagaa
    35341 tcaaaggcga accagaatgg atgcttgaat tccgtctgaa atctctagaa acatttaaca
    35401 aaatgccaat gcaaacatgg ggccccgacc tttcagacat cgatttcgat gccatcaaat
    35461 attaccaaaa accaagtgac aaacccgctc gtgactggga agacgtacca gaaaaaatca
    35521 aagatacatt tgaaaagatt ggtataccag aagctgaacg agcttatcta gccggtgctt
    35581 ccgcacaata tgaatcagaa gtcgtgtacc ataatatgaa agatgaattc gaaaagttag
    35641 gaatcgtctt tactgacact gactctgctt tgaaagaata ccctgaatta ttcaaagagt
    35701 acttttcaaa acttgtaccg ccaaccgaca acaaattagc agcgctgaat tcagccgtat
    35761 ggtcaggtgg gacctttatt tacgtgccaa aaggcgtcaa agtcgatgtt ccattacaaa
    35821 cgtacttccg gatcaatgcg gaaaacacgg ggcaattcga acgtacgctg atcattgttg
    35881 acgaaggagc aagcatccac tatgtcgaag gttgtacggc acctacttat tcaagtaaca
    35941 gcctgcatgc cgccatcgta gaaatcttta caagaaaaga tgcgtattgc cgttacacaa
    36001 cgatccaaaa ctggtcagac aatgtgtaca acttagtaac caaacgagca aaagcatacg
    36061 aaggcgcaac cgttgaatgg atcgacggaa acctaggtgc aaaaacaacg atgaaatacc
    36121 caagtgttta cttagatgga aaaggtgcac gaggcaccat gctctccatc gcctttgcag
    36181 gagcaaacca aatccaagat acgggtgcaa aaatgatcca caatgcacca aatacatcta
    36241 gctctatcgt ctctaaatca atttcaaaag acggcggaga agtaaactat cgaggacaag
    36301 taacctttgc caaaaacagt gcgggttcga tctctcatat cgaatgtgac acgatcatca
    36361 tggacgaatg gtcgaaatcc gatacgatcc cattcaatga gatccacaac agccaagtct
    36421 ctttagaaca cgaagccaaa gtctcaaaaa tctcagaaga acaactctac tatctgatga
    36481 gccgaggcct gaccgaatct gaagcaactg aaatgatcgt catgggcttt gttgaaccat
    36541 tcaccaaaga acttccaatg gaatatgcag tcgaactcaa tcgattgatc agttatgaga
    36601 tggaaggcag tgttgggtaa acctgttata ggagctagaa cccttgatat a
//
LOCUS       DAZKOM010000015        35903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000015 DAZKOM010000000
VERSION     DAZKOM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.015"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..458
                     /locus_tag="ACG8NF_000800"
     CDS             60..458
                     /locus_tag="ACG8NF_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0471306.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            695..1237
                     /locus_tag="ACG8NF_000801"
     CDS             695..1237
                     /locus_tag="ACG8NF_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0471307.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1320..1871)
                     /gene="lepB"
                     /locus_tag="ACG8NF_000802"
     CDS             complement(1320..1871)
                     /gene="lepB"
                     /locus_tag="ACG8NF_000802"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0471308.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2023..2250)
                     /locus_tag="ACG8NF_000803"
     CDS             complement(2023..2250)
                     /locus_tag="ACG8NF_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0471309.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2247..2765)
                     /gene="msrA"
                     /locus_tag="ACG8NF_000804"
     CDS             complement(2247..2765)
                     /gene="msrA"
                     /locus_tag="ACG8NF_000804"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0471310.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2785..3498)
                     /locus_tag="ACG8NF_000805"
     CDS             complement(2785..3498)
                     /locus_tag="ACG8NF_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0471311.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3501..4397)
                     /locus_tag="ACG8NF_000806"
     CDS             complement(3501..4397)
                     /locus_tag="ACG8NF_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0471312.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4555..5397)
                     /locus_tag="ACG8NF_000807"
     CDS             complement(4555..5397)
                     /locus_tag="ACG8NF_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0471313.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5529..6182
                     /locus_tag="ACG8NF_000808"
     CDS             5529..6182
                     /locus_tag="ACG8NF_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0471314.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6245..6772)
                     /locus_tag="ACG8NF_000809"
     CDS             complement(6245..6772)
                     /locus_tag="ACG8NF_000809"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0471315.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6792..7739)
                     /locus_tag="ACG8NF_000810"
     CDS             complement(6792..7739)
                     /locus_tag="ACG8NF_000810"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0471316.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7751..9634)
                     /locus_tag="ACG8NF_000811"
     CDS             complement(7751..9634)
                     /locus_tag="ACG8NF_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0471317.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(9797..11875)
                     /locus_tag="ACG8NF_000812"
     CDS             complement(9797..11875)
                     /locus_tag="ACG8NF_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0471318.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11888..12163)
                     /locus_tag="ACG8NF_000813"
     CDS             complement(11888..12163)
                     /locus_tag="ACG8NF_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471319.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12525..12980)
                     /locus_tag="ACG8NF_000814"
     CDS             complement(12525..12980)
                     /locus_tag="ACG8NF_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471320.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13134..14342)
                     /locus_tag="ACG8NF_000815"
     CDS             complement(13134..14342)
                     /locus_tag="ACG8NF_000815"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0471321.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14454..14786)
                     /locus_tag="ACG8NF_000816"
     CDS             complement(14454..14786)
                     /locus_tag="ACG8NF_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0471322.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14934..15809
                     /locus_tag="ACG8NF_000817"
     CDS             14934..15809
                     /locus_tag="ACG8NF_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0471323.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15891..16472)
                     /locus_tag="ACG8NF_000818"
     CDS             complement(15891..16472)
                     /locus_tag="ACG8NF_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0471324.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16478..17737)
                     /locus_tag="ACG8NF_000819"
     CDS             complement(16478..17737)
                     /locus_tag="ACG8NF_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0471325.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17739..18785)
                     /locus_tag="ACG8NF_000820"
     CDS             complement(17739..18785)
                     /locus_tag="ACG8NF_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471326.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19020..19295)
                     /locus_tag="ACG8NF_000821"
     CDS             complement(19020..19295)
                     /locus_tag="ACG8NF_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0471327.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19538..20848)
                     /gene="der"
                     /locus_tag="ACG8NF_000822"
     CDS             complement(19538..20848)
                     /gene="der"
                     /locus_tag="ACG8NF_000822"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0471328.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(21030..22259)
                     /gene="rpsA"
                     /locus_tag="ACG8NF_000823"
     CDS             complement(21030..22259)
                     /gene="rpsA"
                     /locus_tag="ACG8NF_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0471329.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22394..23074)
                     /gene="cmk"
                     /locus_tag="ACG8NF_000824"
     CDS             complement(22394..23074)
                     /gene="cmk"
                     /locus_tag="ACG8NF_000824"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0471330.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23123..23758)
                     /locus_tag="ACG8NF_000825"
     CDS             complement(23123..23758)
                     /locus_tag="ACG8NF_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0471331.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23824..25227)
                     /locus_tag="ACG8NF_000826"
     CDS             complement(23824..25227)
                     /locus_tag="ACG8NF_000826"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0471332.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(25214..26245)
                     /locus_tag="ACG8NF_000827"
     CDS             complement(25214..26245)
                     /locus_tag="ACG8NF_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471333.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26276..26497
                     /locus_tag="ACG8NF_000828"
     CDS             26276..26497
                     /locus_tag="ACG8NF_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0471334.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26662..27399)
                     /locus_tag="ACG8NF_000829"
     CDS             complement(26662..27399)
                     /locus_tag="ACG8NF_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0471335.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27552..28892)
                     /locus_tag="ACG8NF_000830"
     CDS             complement(27552..28892)
                     /locus_tag="ACG8NF_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0471336.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29129..30652)
                     /locus_tag="ACG8NF_000831"
     CDS             complement(29129..30652)
                     /locus_tag="ACG8NF_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0471337.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(31030..31641)
                     /locus_tag="ACG8NF_000832"
     CDS             complement(31030..31641)
                     /locus_tag="ACG8NF_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0471338.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31764..31889)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31971..32687)
                     /locus_tag="ACG8NF_000833"
     CDS             complement(31971..32687)
                     /locus_tag="ACG8NF_000833"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0471339.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32693..33262)
                     /gene="scpB"
                     /locus_tag="ACG8NF_000834"
     CDS             complement(32693..33262)
                     /gene="scpB"
                     /locus_tag="ACG8NF_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0471340.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33246..34016)
                     /locus_tag="ACG8NF_000835"
     CDS             complement(33246..34016)
                     /locus_tag="ACG8NF_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0471341.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(34013..34384)
                     /locus_tag="ACG8NF_000836"
     CDS             complement(34013..34384)
                     /locus_tag="ACG8NF_000836"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0471342.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34403..35290)
                     /gene="xerD"
                     /locus_tag="ACG8NF_000837"
     CDS             complement(34403..35290)
                     /gene="xerD"
                     /locus_tag="ACG8NF_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0471343.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35386..35859)
                     /locus_tag="ACG8NF_000838"
     CDS             complement(35386..35859)
                     /locus_tag="ACG8NF_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0471344.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT        10089 a         7513 c         6090 g        12211 t
ORIGIN      
        1 tgacagcgtt tcctaaataa gagatgatag acgaacaaaa cagaaaggag acgaaacaaa
       61 tgaatagtat ggtttttgtt aattttcctg ttgctgatgt caatcgttcc gttgcttttt
      121 atgaaaagct gggttttaaa aaaaatgaag aattttctac tgaagaagct ggttcaatgg
      181 tatgggatga cactttttgg ataatgcttc tctctcatga tttttataaa aaatttataa
      241 aagataaaga aatagctgat aataaaaaaa caagtggtgc tttgatttcg tttagtgttg
      301 aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa agaaaatggt ggtactttcc
      361 acgaagtaga tatgggaatg cctgaagaag aaatgttttc attagaagtc caagatctag
      421 acggaaatac attagaaccc gtatggatga agatgtaagc agataactaa tctgacgttt
      481 tttataacaa aaaaacaaag gcggatgata cgataagtat tctccgcctc tatttttttt
      541 attcgtacaa tctctttttt acagtttata aaagcagtct tctcaccctt tttttaattg
      601 ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca cattggtgat tttatggtat
      661 ccttttatta ttaactgaca aaggagagaa tattttgaac actttgattg caaaagacgt
      721 ttttagattg cccgaaatga atgcaagatt gttgtcgacc tctgctcttt tattagccct
      781 tgatgtcatc ttatacaaga tagcgattgg tcctgcttat tttcaaatca cattcggatt
      841 tttaagtatg gcgcttatgg gttatctata tggaccgatc tgggctggtt tgcttgaaat
      901 gttgtcgaac actttaaatt tcacgatatt tggttcagga acgtttcaag ctgcttttct
      961 tatcacggct ttccttggtg gatttattaa tggcttattt ttatatcata aaaagataaa
     1021 cctgccttcc gtcatcgctg cccaattatt gattatgatc ccagtcagcc tttttatcaa
     1081 ttcttggctg atgtctgttt tgtttggtgc agatttcaaa gtaatttttc taggtagatt
     1141 catcaggaat atgatccaaa tccctattca aataatggtt gtttattcat ttatcaaaac
     1201 tttagaggct agaaaagtgt ttaaacgagc tgtttgattt tacgtactaa ggtagtaaac
     1261 tgattttgga ttaaacaata aagcgtgcag tctcaacaga ctgcacgctt tattgtttct
     1321 cactggatcc attttatttc atcaagtgga taatagacaa atcttgcttt acctagaata
     1381 tcagttttat tgatcgtgcc aaacgaacga ctatcttttg aaactcttcg attatctcca
     1441 agtacaaaat aattatcttc cggaagcttg gattgtcccg ttagctcttg tagtgtaaag
     1501 tccgtcgtat aggatgcatt tgcatgatct gatttcaggt ttttagttag ataaggttcc
     1561 ttaactactt tcccattgat cttcaattga tcattttggt agctgacatt ttctcctgga
     1621 agaccgatta ctcgtttgat ataaatcgtg ccatctgcta attgaaaaac aacaatatca
     1681 aatcgtctga tctccgaaaa tttttccatc acaaccatat caccttgctt caacacgttt
     1741 tccattgaat tgccttccac tggtaccggg atgaataaaa atccccgcaa catgaacgca
     1801 aggaatacag aaacaagtaa gtatttgaat acaagccaga agcgattaat atagcgttgt
     1861 ttttttgtca atctctatca atccatccct ctttttttca ttctttcttt attataagaa
     1921 aaatatcaaa aaaatgcgac tagatagctg aaataaaaat aattctttca attaggctat
     1981 tggacaaaaa acttttttga tcataaagta caaaactatc tcttatatgt catgattatt
     2041 ttctaaatat ttttcccatg cttcgtcaaa gatatccatg gacggcaaat agtcgacatt
     2101 cagttccaga taattagaaa tttcttcgta gctagagctg tgcttaggaa attggatgtc
     2161 tttaccggct tctgtagcga aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt
     2221 cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc tccaattttc ttcgataaag
     2281 gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat gctttttata atactcttga
     2341 tggtcttctt ctgctagata aaatggttgg gctggttcga tttttgtaac gattggctgt
     2401 tcgaaacgtc cactttcagc taactgtttc ttactttttt cagcaatgat ccgctgttct
     2461 tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa
     2521 gcgtcagttg gatcagtttg ctgccagtaa agttctacta attgttcata tgaaatcaat
     2581 gttggatcat agataatctc cactgcttct gtatgtccag tcgtttggct ttttacttgt
     2641 tcataagtcg gatctggtac atgccctcca gtatatcctg acatcactgt atggatacca
     2701 ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc ctcctgcaaa tattgctcgt
     2761 tccattatga ttccccctct aatactactc atccgatgat tttttatttt cttttgtctt
     2821 gctttcagta ctgctggtgc tagttttgtt ggtatttgag ttgtttgaag catcagaggt
     2881 atcttttggc aaatatagac taaaccgaat gtcatcattt actaagtcga ttttatctgc
     2941 tttaatatac atcccgtttt gcattcggaa ttgatccaac ctaagcatga tagtcagatc
     3001 atctgtattg acttcgatcc aatctggtaa tttatagttt ctcttgatca tattcatcac
     3061 atctttgatt ggcaaactca atgttccgat agaaagactt ctagctttta gttggacatt
     3121 tccgttttcc atcacatacg gatcaaaata taaatagaaa tcgataggaa aacctagtac
     3181 ttcgaaagta ccattgagta gtgcttcatt ttccaaataa aatttatatt caatatcact
     3241 atctttttga tactcactaa ggaaaaagct gatgatttga ttgacttttt ctttatttga
     3301 attgatcgtt aagacaggat ctccttgtct ttccgtaaga gctggtattt ctgggagttc
     3361 tggttcccga ttagcaaaaa tacggctgcc taaaaatacg gtcgaaccaa gtactacccc
     3421 aagaaggata aggaaggcga atttccagta atttgttttc tttgttttgt ttgaagatac
     3481 gtcagatctt gttttcatgt ttattgatca tcctccttct ctaaccattg gttttgtgtc
     3541 tcgatcagct gatctctcac tgcatttgcc atcaattgat aacctaaatt atttggatgg
     3601 aaatgatctt cttcatagag cacattattt ttgatgccat ttagattttc tgtcgtgcta
     3661 gttgtttgat ctgttccttc ttctgtgaca cctacttcct gattcaatcc ttgataaagc
     3721 aaatcattga ttgggataaa gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct
     3781 ttgttccaat tgttaacaat tgtctgcatg tcagtgatct caggaaagtt caaataaaat
     3841 ggattgtaga tgcctaaaat gtaaataggt gcggttttat tgagggatcg aaattcttcc
     3901 aatagttctg tgacattttc ctgatagttt ttcagtggct ttttaaagga attgaccgtt
     3961 aacccgaata aattattttg gataactttc atcaaatcat taccgccaac agtcagtgtg
     4021 ataaaatcag ctgttttgat attgtttcga atttcttctt ttgtttttac tcgctttaag
     4081 atctggtcac ttcgttcacc atttacacca tagttttctg tttcaaccgc ggtcaaatta
     4141 taacggtctt ttagatcatt tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct
     4201 cctactcctt cagtcagcga atctccgata gctatgtagt gaagtttttc ttttttattt
     4261 ggatcttctc tcacagtagt tactggtttc attagtgggc cagctttcgg tatagaagca
     4321 gctaaacctg catagatgat gatcgcaatt aataatggga acaaaacgag gatcaaatgt
     4381 cgtttcgtta atttcatttt ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa
     4441 ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat tattttccta attttataaa
     4501 gaaaaagaga ataagctgca agcctttcag gctgagggct tatcctcttt tcgactaatc
     4561 tacataatac atgattgcaa acgcgtcttt accagtatgc gtagcgataa ccggattcgt
     4621 atgcagtaca gggatatgca tgtccgggaa taattcttgc aaaccctctt tgaattcttg
     4681 cgccaattct tttgcatccg catgtgaaat accgatttgt cgcacatttg gcaattcttt
     4741 cagttcattt ttcaaattat taaaccattt atcgaacgtt ttctttcctc gccccttagt
     4801 tacagggatt aattcagaat gggcgaaatt catcaccacg cgtatgttca aaacactaga
     4861 cagcaaacca gttgttcggc tgattcttcc accttttacc aagttatcta gtgtggatat
     4921 tccgatgtat aattttgtat tgtcttgtac ttttttgact tcttcaagga ttttctctac
     4981 tgttttacct tcttttgcta attcagctgc tcgaattacc tgaaaagata atccttggtc
     5041 agtggtatca ctgtcgataa ccgttacttt cgtttttgtt agattactgg cttgtcttgc
     5101 tgcttctact gttccactca agccttttgt catatggatc gaaatcactt cactgccgtc
     5161 ttctcctagc tcatcataca tcgagatgaa ttctccaatt ggtggttggc ttgttttggg
     5221 cagatttttt gctttcgcca tcatttccat aaatctttcg ccgtctagtt ggtcatcatc
     5281 gggatatact gttccgtcga tcatgatgga tagcggcatg acacgaatgc ctaaagcatc
     5341 gcgtacattt ttttccatcg tacatgaaga atctgttacg atttttacat ttgtcattat
     5401 tttctctctc tctttcttta acaagatgcc ttattcatgt taaactacat gtaagacaat
     5461 ttctattttt agtataacag acaaataatg atttttcatt aattacacct agtaaaggag
     5521 ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta
     5581 atgcagtcac ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag
     5641 taaaaggtgc ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga
     5701 tgatgattct tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa
     5761 aaaaggtgtt ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata
     5821 ctcctttttg tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct
     5881 ggttgtttgc gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag
     5941 tatcaaaagt atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg
     6001 ttcctcttta tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt
     6061 cttacacgat aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt
     6121 ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt
     6181 aataaaaaaa ataaagctat ggatgggaca gctctaaata acagctagtc tatccatagc
     6241 ttttttatat cgtcaattcg cctgtcccgc tttttacagg attctcatta cgctgatacg
     6301 tttcaaattg atgcggataa cgattttttt catccatttc tccttgacta gtattgatca
     6361 gtgaccaatc agaccagtca acaggtggaa aataagcatc tccatcgaat acatgatgga
     6421 tgactgtacg gtataatatc gtacaaaatg gcagaaattc ttgataaaca gctgaccctc
     6481 cagcgatgaa aaagataccg tctgtttctt tttccagagc aagcacgtct tccacactat
     6541 gcatgacatg cacgccttct gctttatagg aagaatctct cgtcaaaaca atcgtctgac
     6601 gattcggcaa cggacggcta cccatccctt cgaatgtttt tcgtcccatc actagtgtat
     6661 ttgcttctgt catttgtttg aagaatttca gatcatttgg caaacgccaa ggaagacggt
     6721 cttcttttcc aatcaatccg ttttcatcct gtgcccaaat tgcagctaac attttctcat
     6781 cctttctgat tctaaacagc gataggagct ttgatcgccg gatgtgggtc atatccttct
     6841 aattggatat cttccatctc aaaatcaaat actgactgct tttcctgatt caaaactaaa
     6901 gtcggaaatg cgcgtatttc tcggctcaat tgttctttca tttgttcgat atgattgata
     6961 taaagatgag catctcctaa tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt
     7021 gcaattaggt gagtcagcaa cgcataactt gcaatgttga acggaacgcc tagaaacaca
     7081 tccccactgc gttgatacaa ttggcagctt agccgtccct cttgaacata aaattgaaac
     7141 atcgtatgac aaggaggcaa agccatgcta ggaacatctt ccggattcca tgcagaaacg
     7201 atcaatcttc tagaatcagg agtctttttg attgcttcga tcacttgttt gatttgatcg
     7261 ataaagccac cgtcttttgt ttcccagtga cgccattgat agccgtagat gtgtccaaga
     7321 tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc
     7381 tgctttttat attcttcatt gaattcagga tctgtcaaac atctatggcc aaaatcagtc
     7441 atatctggtc cattatagtc tggacttttg atgtagcgtt caaatgccca ttcatcccaa
     7501 atatggtttt tattttgtaa tagatagcgg atattcgtat ctccttttaa aaaccaaagc
     7561 aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt
     7621 tgcagatcaa accgcatttg gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct
     7681 tctttacgat gcccttcttc caacaatttt cttcctaagt caagatacgc ttgttccatc
     7741 ctttttcctc ctaatcttct acccattcac tcaagtattc ccaccgtgcc attttttctt
     7801 caagttgtgc ttctgtttca gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat
     7861 ctccttgatg attcatctct tcttggagca atgagatttt ttcttcaagt tctgcaatct
     7921 catcttcaat cgtttcccat tctttttgtt ccatgtaaga taatttcttc ttttcagcag
     7981 gttccttgct ctgtattttt ggtttcgctt tttcagcagg ctgctgggtc tctttttgct
     8041 tcagtaaata actggacatg tttccgtaat aaagttcttg ttttccgttt ccttggaata
     8101 ctaataactt atctgctacc ttgtccaaga aataacggtc atgggaaaca gtgatcacag
     8161 cacctggaaa acttctaaaa taatcttcca aaatcgtcaa tgttgctatg tccaaatcat
     8221 tggtcggttc atccaataat aaaacattag gttgctgaat cagcagtttc aacaaaaata
     8281 accggcgttt ttctccgcct gaaagtttgc ctatcagtgt cccatgcata aatcttggaa
     8341 ataaaaatcg ttcaagtaat tctgctactc cgatttgact gccatctgct gtttttgctt
     8401 cttcagcagc ttcttgtaag taagcaatca tccgtttatc tggtgccatt tcttcatttt
     8461 cttgtgtata atacgcaaga tgaaccgttt caccgataga cattgtaccg ctatccaggg
     8521 gaattctgcc agctagaata ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa
     8581 tacctagtct ttcccgagat tgaatcaata aatctaaatg ttctagcagt gtttggtcat
     8641 ttatacggta actgccatct ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta
     8701 ggttcagctc taaatcatct tcttggttta cttggtacaa gttctccttt agatcttcga
     8761 aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag
     8821 ctaattcctg tttataaagc cgcttgcgct tttcttcttg ttctacttct acacgatcac
     8881 gttctgcttt ttctaagacg taagcttcat aatttccttt atactcgtat aattttccaa
     8941 atgaaagttc gaagattcgg tttgctactc tatctagaaa ataacgatcg tgggtaacca
     9001 tcaatagtgc gccacggtat tgtttcaaat agttttccaa ccactcgatc gcttcataat
     9061 ctaaatggtt tgttggttcg tctagtaata gtaaatcggg ttcatcaatc aacacttgcg
     9121 ccagactaac tcgttttttc tgaccacctg ataaagtact gattttttta tctaattcac
     9181 tgattcctaa tttttgcaaa ataatctttg cgttggtatc tgtagtccaa gcatcttctt
     9241 tattcatttg ttcttctgct ttagcataac gtttttgcac atcagggtct tctccgtttt
     9301 cccctagttc gatcaatgct tcctcatatg ctcgaacagt ttggatcaag gggctgtttc
     9361 cttgaaagac tgcttggaga acagttaatt catcggaaaa ttcagctgct tgagaaagat
     9421 aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga
     9481 taccggcaat aatatttaag agacttgttt tacccgtccc gttcgtccca atcaacccga
     9541 tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa caaatctttt tcaccataag
     9601 tttttttgag gtcagttact tttaattctt tcaacatgtt ccctccttat cttggatatt
     9661 gtatcataaa aatagtgaac ttcaggaaaa tttttttggt atttgttctt tttgtgttct
     9721 caaaaaaaga acaggaccga ttcgatcctg ttctgatatg ttttgttttt tataaaactt
     9781 gaaaattttt acctttttat tttattggac tcgttttttt tgactttgct cgaaacgcaa
     9841 atcggaaaga aaagttatca gatggtttgt tcatcgtata agattctaac aaattgatat
     9901 tcctgcgata gctgttcgca gaaaacgtcg tgattttttc agcaacttcg tattcatcaa
     9961 tgatttttct ttcaagataa taacctctta gcattttttt cgtaaacaga ctatcttgct
    10021 ggatcatcca cgaagaaatc aaattccccg agttcatttt tattccttca agtttgcgtt
    10081 gctggataaa aaattgaacc atcgtttcat cataaatatc agtcaagtta tctaaacttt
    10141 caataatcaa ctgagtattt ttttgatatg tctctctgat ttcttggata tcttcatcac
    10201 taataaatgt ctgacgatca gcatatcttc tttgccaaaa cattttcgga tggagaacga
    10261 ttcgcattaa cctacggaca acaatgaacc aaaagccgat aaaagacaaa atctggcttg
    10321 tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa acgataacca ttagaagaaa
    10381 gcttgcccga acgataactt tcatccaatc catcccgttc gatcgacaaa atcagtgctt
    10441 gtatttcttg tacttcttgc ttttcatcat cggttaaatc ttccaagtac agatcacgta
    10501 tccgttcttg ataattttct atcactgctt cagtggctaa aaattctttt gtattacgat
    10561 caatttcctt tatttctttt cgcaaaactt caacgacctc ttctaaaatc atcaaagcat
    10621 tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat aggcaagaca atgatcccta
    10681 tgaccaaagt cactaagatg acacccgccg tcagaaacaa aagcaaagac cgctggtaaa
    10741 acatcatatt attgattgaa aatggcaaaa taaaaatcgt agccaaactg actgttccct
    10801 ttacaccgcc aaatgttaag atcaagatct cgttcaactg ttttttgaac tttttactgc
    10861 catctttgaa tacataaaaa agactgatag aaatgaatct gatcacaaaa agcatgacag
    10921 agattaatac gataatcgct aataaaagac cgtttggata taaaccgtct ccccatactg
    10981 gcgagaacac ttgcgtcaat tcgattccta aaaatataaa tactaatgca ttcagtgtaa
    11041 aagcaatagt tgtccatgta ctgtgggaca agctcgacaa ttctgcatca aacacagaga
    11101 ttttcctaaa accagaagct tggagaatac ctgctgccac tgcagcaatg attccagata
    11161 catcaaatgc ctctgccaat acataggcta gaaaaggaag caaaagttcg atcagcagat
    11221 accctgtgac atcttgtgct gatgcttttt cgatcaaatg aattattttt tgtttgaacc
    11281 agacaagcaa aaagccgatc aaagctcccc caatactaga aactactaga ctcatccccg
    11341 cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag agcaaactgg aaggcagtaa
    11401 caccagatgc atcattcaaa agaccttctc cttccaaaat atgcatcgcc tttttaggga
    11461 tattcactcg tccagaaaga gaactgactg ctaccgcatc tgtcggacct aacgctgctc
    11521 caaaagcaaa gcaggcagca agtgggacac ttggcaacag gaatgataac gtcgcaccta
    11581 ccccgacaag ggtcaaaatg actcctccaa atgccaagaa caaaattgtc ccgaaatttt
    11641 tgagaatcga agaaatatct gctttttctc cttctctaaa taacaatggt gcaataatca
    11701 taactaagaa caattcaggt tcaaaatcga ttgattgtcc ccactctgtc agacctaata
    11761 ccactccaag gaaaatttgg atcagtggag ctggaatcat tggcaaaatg cggctagcta
    11821 tgttcgataa ggtgatcgtt actgcaaata caataactaa ctcagcaaat tccatcgtcc
    11881 atccccttta ttttttattt tgtgtaagca tttttaaaaa ttgttctttt ttctttaaaa
    11941 ttttggtttc gactacttca acctgtgttt ggttcccctc taattcacac tgtacacgtt
    12001 ttgactccaa acgaagcaga tcatgtttca gacgttccaa ctgacgatac atcaatatat
    12061 ttcttttacg caacttacaa agtatttgac ggcggtcatc catcgaaagg gcgccatagc
    12121 gatcatgcaa cagttttttt tgttgttcaa aactctctct cattttccag atctcctata
    12181 cacaaacttc catcattata gcaaagttct ttttggatga aaaatatttc attgtttcat
    12241 cgtttattgc tagttagaaa tttccataaa tttttcaacg cctcattcta ttccaaatag
    12301 tatcagatat caagtaaaac ttaagaaaga taaaagtttc tgacaaaagg atgtttactt
    12361 tcccattcct gcctttcttg ttcttttatc acaacaaaaa aatggggctg cgacaaaaat
    12421 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
    12481 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
    12541 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
    12601 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
    12661 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
    12721 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
    12781 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
    12841 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
    12901 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
    12961 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
    13021 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
    13081 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
    13141 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
    13201 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
    13261 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
    13321 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
    13381 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
    13441 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
    13501 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
    13561 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
    13621 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
    13681 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
    13741 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
    13801 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
    13861 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
    13921 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
    13981 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
    14041 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
    14101 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
    14161 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
    14221 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
    14281 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
    14341 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
    14401 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
    14461 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
    14521 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
    14581 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
    14641 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
    14701 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
    14761 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
    14821 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
    14881 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
    14941 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
    15001 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
    15061 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
    15121 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
    15181 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
    15241 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
    15301 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
    15361 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
    15421 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
    15481 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
    15541 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
    15601 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
    15661 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
    15721 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
    15781 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
    15841 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
    15901 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
    15961 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
    16021 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
    16081 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
    16141 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
    16201 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
    16261 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
    16321 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
    16381 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
    16441 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
    16501 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
    16561 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
    16621 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
    16681 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
    16741 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
    16801 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
    16861 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
    16921 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
    16981 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
    17041 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
    17101 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
    17161 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
    17221 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
    17281 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
    17341 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
    17401 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
    17461 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
    17521 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
    17581 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
    17641 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
    17701 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
    17761 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
    17821 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
    17881 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
    17941 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
    18001 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
    18061 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
    18121 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
    18181 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
    18241 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
    18301 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
    18361 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
    18421 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
    18481 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
    18541 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
    18601 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
    18661 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
    18721 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
    18781 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
    18841 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
    18901 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
    18961 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
    19021 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
    19081 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
    19141 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
    19201 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
    19261 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
    19321 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
    19381 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
    19441 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
    19501 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
    19561 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
    19621 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
    19681 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
    19741 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
    19801 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
    19861 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
    19921 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
    19981 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
    20041 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
    20101 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
    20161 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
    20221 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
    20281 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
    20341 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
    20401 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
    20461 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
    20521 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
    20581 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
    20641 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
    20701 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
    20761 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
    20821 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
    20881 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
    20941 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
    21001 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
    21061 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
    21121 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
    21181 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
    21241 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
    21301 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
    21361 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
    21421 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
    21481 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
    21541 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
    21601 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
    21661 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
    21721 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
    21781 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
    21841 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
    21901 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
    21961 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
    22021 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
    22081 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
    22141 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
    22201 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
    22261 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
    22321 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
    22381 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    22441 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    22501 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    22561 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    22621 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    22681 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    22741 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    22801 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    22861 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    22921 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    22981 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    23041 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    23101 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    23161 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    23221 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    23281 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    23341 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    23401 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    23461 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    23521 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    23581 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    23641 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    23701 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    23761 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    23821 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
    23881 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    23941 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    24001 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    24061 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    24121 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
    24181 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    24241 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    24301 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    24361 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    24421 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    24481 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    24541 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    24601 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    24661 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    24721 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    24781 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    24841 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    24901 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    24961 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    25021 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    25081 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    25141 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    25201 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    25261 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    25321 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    25381 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    25441 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    25501 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    25561 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    25621 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    25681 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    25741 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    25801 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    25861 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    25921 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    25981 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    26041 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    26101 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    26161 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    26221 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    26281 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    26341 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    26401 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    26461 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    26521 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    26581 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    26641 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    26701 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    26761 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    26821 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    26881 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    26941 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    27001 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    27061 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    27121 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    27181 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    27241 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    27301 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    27361 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    27421 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    27481 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    27541 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    27601 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    27661 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    27721 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    27781 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    27841 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    27901 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    27961 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    28021 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    28081 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    28141 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    28201 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    28261 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    28321 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    28381 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    28441 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    28501 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    28561 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    28621 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    28681 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    28741 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    28801 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    28861 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    28921 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    28981 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    29041 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    29101 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    29161 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    29221 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    29281 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    29341 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    29401 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    29461 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    29521 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    29581 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    29641 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    29701 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    29761 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    29821 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    29881 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    29941 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    30001 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    30061 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    30121 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    30181 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    30241 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    30301 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    30361 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    30421 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    30481 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    30541 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    30601 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    30661 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    30721 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    30781 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    30841 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    30901 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    30961 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    31021 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    31081 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    31141 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    31201 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    31261 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    31321 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    31381 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    31441 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    31501 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    31561 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    31621 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    31681 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    31741 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    31801 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    31861 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    31921 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    31981 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    32041 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    32101 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    32161 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    32221 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    32281 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    32341 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    32401 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    32461 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    32521 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    32581 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    32641 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    32701 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    32761 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    32821 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    32881 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    32941 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    33001 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    33061 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    33121 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    33181 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    33241 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    33301 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    33361 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    33421 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    33481 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    33541 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    33601 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    33661 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    33721 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca
    33781 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    33841 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    33901 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    33961 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    34021 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    34081 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    34141 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    34201 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    34261 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    34321 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    34381 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    34441 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    34501 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    34561 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    34621 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    34681 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    34741 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    34801 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    34861 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    34921 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    34981 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    35041 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    35101 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    35161 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    35221 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    35281 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    35341 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    35401 tcttttttct ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt
    35461 tactaaaaaa tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc
    35521 tacttcctcg atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc
    35581 ttcttttctc aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt
    35641 caattctgtc aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt
    35701 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    35761 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    35821 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    35881 taataattac aaataaaaaa aga
//
LOCUS       DAZKOM010000016        35882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000016 DAZKOM010000000
VERSION     DAZKOM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.016"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8NF_000839"
     CDS             <1..1152
                     /locus_tag="ACG8NF_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0471345.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8NF_000840"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8NF_000840"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0471346.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8NF_000841"
     CDS             2728..3003
                     /locus_tag="ACG8NF_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0471347.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8NF_000842"
     CDS             3009..3776
                     /locus_tag="ACG8NF_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0471348.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8NF_000843"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8NF_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0471349.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8NF_000844"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8NF_000844"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0471350.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8NF_000845"
     CDS             7920..8222
                     /locus_tag="ACG8NF_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0471351.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8NF_000846"
     CDS             8225..9388
                     /locus_tag="ACG8NF_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0471352.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8NF_000847"
     CDS             9414..12842
                     /locus_tag="ACG8NF_000847"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0471353.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8NF_000848"
     CDS             12863..13987
                     /locus_tag="ACG8NF_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0471354.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8NF_000849"
     CDS             13968..14414
                     /locus_tag="ACG8NF_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0471355.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8NF_000850"
     CDS             14398..14736
                     /locus_tag="ACG8NF_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0471356.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8NF_000851"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8NF_000851"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0471357.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8NF_000852"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8NF_000852"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0471358.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8NF_000853"
     CDS             15893..16936
                     /locus_tag="ACG8NF_000853"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0471359.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8NF_000854"
     CDS             17095..17823
                     /locus_tag="ACG8NF_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0471360.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8NF_000855"
     CDS             17939..18184
                     /locus_tag="ACG8NF_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0471361.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8NF_000856"
     CDS             18181..18399
                     /locus_tag="ACG8NF_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0471362.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8NF_000857"
     CDS             18535..19074
                     /locus_tag="ACG8NF_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0471363.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8NF_000858"
     CDS             19067..21340
                     /locus_tag="ACG8NF_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0471364.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8NF_000859"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8NF_000859"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0471365.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8NF_000860"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8NF_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0471366.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG8NF_000861"
     CDS             complement(23167..23436)
                     /locus_tag="ACG8NF_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471367.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG8NF_000862"
     CDS             complement(23698..23826)
                     /locus_tag="ACG8NF_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471368.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG8NF_000863"
     CDS             complement(23906..24361)
                     /locus_tag="ACG8NF_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0471369.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG8NF_000864"
     CDS             complement(24499..26427)
                     /locus_tag="ACG8NF_000864"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0471370.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACG8NF_000865"
     CDS             complement(26624..27457)
                     /locus_tag="ACG8NF_000865"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0471371.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACG8NF_000866"
     CDS             complement(27649..28353)
                     /locus_tag="ACG8NF_000866"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0471372.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACG8NF_000867"
     CDS             complement(28376..29002)
                     /locus_tag="ACG8NF_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0471373.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACG8NF_000868"
     CDS             29328..31703
                     /locus_tag="ACG8NF_000868"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0471374.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACG8NF_000869"
     CDS             complement(31727..32218)
                     /locus_tag="ACG8NF_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0471375.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACG8NF_000870"
     CDS             32405..33325
                     /locus_tag="ACG8NF_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0471376.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACG8NF_000871"
     CDS             33479..33823
                     /locus_tag="ACG8NF_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0471377.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACG8NF_000872"
     CDS             complement(34013..34513)
                     /locus_tag="ACG8NF_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0471378.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACG8NF_000873"
     tRNA            35041..35124
                     /locus_tag="ACG8NF_000873"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="ACG8NF_000874"
     CDS             complement(35448..>35882)
                     /locus_tag="ACG8NF_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0471379.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7404 g        10348 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAZKOM010000017        32706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000017 DAZKOM010000000
VERSION     DAZKOM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.017"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8NF_000875"
     CDS             33..656
                     /locus_tag="ACG8NF_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471380.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8NF_000876"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8NF_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0471381.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8NF_000877"
     CDS             2152..3033
                     /locus_tag="ACG8NF_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471382.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8NF_000878"
     CDS             3036..3806
                     /locus_tag="ACG8NF_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471383.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8NF_000879"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8NF_000879"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0471384.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8NF_000880"
     CDS             5117..6280
                     /locus_tag="ACG8NF_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0471385.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8NF_000881"
     CDS             6397..6807
                     /locus_tag="ACG8NF_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0471386.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8NF_000882"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8NF_000882"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0471387.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8NF_000883"
     CDS             9380..9664
                     /locus_tag="ACG8NF_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471388.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8NF_000884"
     CDS             9824..10060
                     /locus_tag="ACG8NF_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0471389.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8NF_000885"
     CDS             10057..10455
                     /locus_tag="ACG8NF_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0471390.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="ACG8NF_000886"
     CDS             complement(11122..12066)
                     /locus_tag="ACG8NF_000886"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0471391.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="ACG8NF_000887"
     CDS             complement(12362..13306)
                     /locus_tag="ACG8NF_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0471392.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13526..14740
                     /locus_tag="ACG8NF_000888"
     CDS             13526..14740
                     /locus_tag="ACG8NF_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0471393.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14762..16777
                     /locus_tag="ACG8NF_000889"
     CDS             14762..16777
                     /locus_tag="ACG8NF_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0471394.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16860..18233)
                     /locus_tag="ACG8NF_000890"
     CDS             complement(16860..18233)
                     /locus_tag="ACG8NF_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0471395.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18259..18543)
                     /locus_tag="ACG8NF_000891"
     CDS             complement(18259..18543)
                     /locus_tag="ACG8NF_000891"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0471396.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18545..20560)
                     /locus_tag="ACG8NF_000892"
     CDS             complement(18545..20560)
                     /locus_tag="ACG8NF_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0471397.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20739..21044)
                     /locus_tag="ACG8NF_000893"
     CDS             complement(20739..21044)
                     /locus_tag="ACG8NF_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0471398.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21133..22163)
                     /locus_tag="ACG8NF_000894"
                     /pseudo
     CDS             complement(21133..22163)
                     /locus_tag="ACG8NF_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            22370..23245
                     /locus_tag="ACG8NF_000895"
     CDS             22370..23245
                     /locus_tag="ACG8NF_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0471399.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23397..24239)
                     /locus_tag="ACG8NF_000896"
     CDS             complement(23397..24239)
                     /locus_tag="ACG8NF_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0471400.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24331..26208)
                     /locus_tag="ACG8NF_000897"
     CDS             complement(24331..26208)
                     /locus_tag="ACG8NF_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0471401.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26205..27626)
                     /locus_tag="ACG8NF_000898"
     CDS             complement(26205..27626)
                     /locus_tag="ACG8NF_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0471402.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27629..31018)
                     /locus_tag="ACG8NF_000899"
     CDS             complement(27629..31018)
                     /locus_tag="ACG8NF_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0471403.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31275..32666
                     /locus_tag="ACG8NF_000900"
     CDS             31275..32666
                     /locus_tag="ACG8NF_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471404.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
BASE COUNT        10104 a         5919 c         6267 g        10416 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa atgtctaatt
    13321 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
    13381 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
    13441 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
    13501 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
    13561 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
    13621 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
    13681 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
    13741 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
    13801 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
    13861 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
    13921 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
    13981 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
    14041 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
    14101 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
    14161 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
    14221 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
    14281 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
    14341 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
    14401 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
    14461 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
    14521 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
    14581 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
    14641 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
    14701 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
    14761 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
    14821 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
    14881 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
    14941 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
    15001 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
    15061 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
    15121 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
    15181 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
    15241 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
    15301 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
    15361 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
    15421 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
    15481 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
    15541 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
    15601 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
    15661 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
    15721 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
    15781 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
    15841 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
    15901 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
    15961 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
    16021 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
    16081 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
    16141 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
    16201 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
    16261 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
    16321 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
    16381 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
    16441 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
    16501 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
    16561 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
    16621 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
    16681 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
    16741 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
    16801 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
    16861 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
    16921 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
    16981 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
    17041 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
    17101 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
    17161 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
    17221 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
    17281 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
    17341 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
    17401 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
    17461 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
    17521 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
    17581 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
    17641 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
    17701 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
    17761 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
    17821 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
    17881 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
    17941 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
    18001 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
    18061 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
    18121 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
    18181 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
    18241 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
    18301 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
    18361 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
    18421 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
    18481 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
    18541 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
    18601 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
    18661 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
    18721 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
    18781 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
    18841 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
    18901 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
    18961 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
    19021 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
    19081 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
    19141 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
    19201 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
    19261 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
    19321 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
    19381 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
    19441 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
    19501 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
    19561 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
    19621 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
    19681 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
    19741 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
    19801 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
    19861 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
    19921 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
    19981 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
    20041 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
    20101 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
    20161 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
    20221 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
    20281 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
    20341 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
    20401 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
    20461 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
    20521 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
    20581 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
    20641 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
    20701 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
    20761 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
    20821 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
    20881 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
    20941 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
    21001 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
    21061 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
    21121 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
    21181 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
    21241 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
    21301 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
    21361 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
    21421 ttttgtttgt ttcacgaaca tccctttttt ccgtaaacga cgaaacttgg acgttctttg
    21481 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    21541 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    21601 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    21661 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    21721 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    21781 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    21841 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    21901 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    21961 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    22021 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    22081 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    22141 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    22201 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    22261 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    22321 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    22381 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    22441 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    22501 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    22561 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    22621 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    22681 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    22741 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    22801 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    22861 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    22921 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    22981 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    23041 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    23101 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    23161 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    23221 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    23281 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    23341 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    23401 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    23461 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    23521 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    23581 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    23641 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    23701 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    23761 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    23821 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    23881 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    23941 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    24001 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    24061 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    24121 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    24181 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    24241 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    24301 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    24361 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    24421 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    24481 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    24541 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    24601 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    24661 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    24721 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    24781 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    24841 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    24901 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    24961 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    25021 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    25081 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    25141 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    25201 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    25261 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    25321 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    25381 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    25441 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    25501 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    25561 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    25621 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    25681 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    25741 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    25801 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    25861 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    25921 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    25981 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    26041 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    26101 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    26161 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    26221 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    26281 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    26341 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    26401 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    26461 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    26521 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    26581 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    26641 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    26701 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    26761 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    26821 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    26881 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    26941 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    27001 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    27061 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    27121 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    27181 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    27241 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    27301 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    27361 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    27421 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    27481 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    27541 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    27601 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    27661 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    27721 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    27781 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    27841 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    27901 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    27961 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    28021 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    28081 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    28141 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    28201 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    28261 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    28321 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    28381 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    28441 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    28501 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    28561 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    28621 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    28681 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    28741 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    28801 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    28861 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    28921 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    28981 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    29041 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    29101 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    29161 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    29221 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    29281 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    29341 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    29401 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    29461 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    29521 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    29581 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    29641 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    29701 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    29761 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    29821 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    29881 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    29941 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    30001 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    30061 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    30121 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    30181 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    30241 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    30301 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    30361 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    30421 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    30481 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    30541 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    30601 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    30661 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    30721 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    30781 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    30841 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    30901 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    30961 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    31021 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    31081 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    31141 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    31201 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    31261 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    31321 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    31381 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    31441 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    31501 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    31561 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    31621 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    31681 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    31741 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    31801 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    31861 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    31921 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    31981 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    32041 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    32101 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    32161 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    32221 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    32281 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    32341 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    32401 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    32461 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    32521 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    32581 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    32641 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    32701 ataaaa
//
LOCUS       DAZKOM010000018        32497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000018 DAZKOM010000000
VERSION     DAZKOM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.018"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8NF_000901"
     CDS             complement(<1..563)
                     /locus_tag="ACG8NF_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0471405.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8NF_000902"
     CDS             complement(750..1400)
                     /locus_tag="ACG8NF_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471406.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8NF_000903"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8NF_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0471407.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8NF_000904"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8NF_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0471408.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8NF_000905"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8NF_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0471409.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8NF_000906"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8NF_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0471410.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8NF_000907"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8NF_000907"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0471411.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8NF_000908"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8NF_000908"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0471412.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8NF_000909"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8NF_000909"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0471413.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8NF_000910"
     CDS             12268..13770
                     /locus_tag="ACG8NF_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0471414.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8NF_000911"
     CDS             13772..14509
                     /locus_tag="ACG8NF_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471415.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG8NF_000912"
     CDS             complement(14680..15873)
                     /locus_tag="ACG8NF_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0471416.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG8NF_000913"
     CDS             complement(15952..16158)
                     /locus_tag="ACG8NF_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0471417.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG8NF_000914"
     CDS             complement(16151..17293)
                     /locus_tag="ACG8NF_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0471418.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG8NF_000915"
     CDS             17805..18440
                     /locus_tag="ACG8NF_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0471419.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG8NF_000916"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG8NF_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG8NF_000917"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG8NF_000917"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8NF_000918"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8NF_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0471420.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACG8NF_000919"
     CDS             21086..21253
                     /locus_tag="ACG8NF_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0471421.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACG8NF_000920"
     CDS             complement(21313..21666)
                     /locus_tag="ACG8NF_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471422.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="ACG8NF_000921"
     CDS             22170..22589
                     /locus_tag="ACG8NF_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0471423.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="ACG8NF_000922"
     CDS             22615..22821
                     /locus_tag="ACG8NF_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471424.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="ACG8NF_000923"
     CDS             complement(23049..23222)
                     /locus_tag="ACG8NF_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471425.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23234..23863
                     /locus_tag="ACG8NF_000924"
     CDS             23234..23863
                     /locus_tag="ACG8NF_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471426.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG8NF_000925"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG8NF_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0471427.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG8NF_000926"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG8NF_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0471428.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG8NF_000927"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG8NF_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0471429.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG8NF_000928"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG8NF_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0471430.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG8NF_000929"
                     /pseudo
     CDS             complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG8NF_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(30257..30484)
                     /locus_tag="ACG8NF_000930"
     CDS             complement(30257..30484)
                     /locus_tag="ACG8NF_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0471431.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31575
                     /locus_tag="ACG8NF_000931"
                     /pseudo
     CDS             30968..31575
                     /locus_tag="ACG8NF_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            31748..32461
                     /locus_tag="ACG8NF_000932"
     CDS             31748..32461
                     /locus_tag="ACG8NF_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471432.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DAZKOM010000019        30309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000019 DAZKOM010000000
VERSION     DAZKOM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.019"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1860)
                     /locus_tag="ACG8NF_000933"
     CDS             complement(<1..1860)
                     /locus_tag="ACG8NF_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0471433.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELL"
     gene            complement(2072..2968)
                     /locus_tag="ACG8NF_000934"
     CDS             complement(2072..2968)
                     /locus_tag="ACG8NF_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0471434.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2961..3641)
                     /locus_tag="ACG8NF_000935"
     CDS             complement(2961..3641)
                     /locus_tag="ACG8NF_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0471435.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(3642..4424)
                     /locus_tag="ACG8NF_000936"
     CDS             complement(3642..4424)
                     /locus_tag="ACG8NF_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0471436.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(4417..5334)
                     /locus_tag="ACG8NF_000937"
     CDS             complement(4417..5334)
                     /locus_tag="ACG8NF_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0471437.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(5498..5752)
                     /locus_tag="ACG8NF_000938"
     CDS             complement(5498..5752)
                     /locus_tag="ACG8NF_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471438.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            6609..9836
                     /gene="fss3"
                     /locus_tag="ACG8NF_000939"
     CDS             6609..9836
                     /gene="fss3"
                     /locus_tag="ACG8NF_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0471439.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            9949..10263
                     /locus_tag="ACG8NF_000940"
     CDS             9949..10263
                     /locus_tag="ACG8NF_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0471440.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            10276..10650
                     /locus_tag="ACG8NF_000941"
     CDS             10276..10650
                     /locus_tag="ACG8NF_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0471441.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            10660..10995
                     /locus_tag="ACG8NF_000942"
     CDS             10660..10995
                     /locus_tag="ACG8NF_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0471442.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            11078..12418
                     /locus_tag="ACG8NF_000943"
     CDS             11078..12418
                     /locus_tag="ACG8NF_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0471443.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            12496..13170
                     /locus_tag="ACG8NF_000944"
     CDS             12496..13170
                     /locus_tag="ACG8NF_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0471444.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            13163..13327
                     /locus_tag="ACG8NF_000945"
     CDS             13163..13327
                     /locus_tag="ACG8NF_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471445.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            13403..14545
                     /gene="dcm"
                     /locus_tag="ACG8NF_000946"
                     /pseudo
     CDS             13403..14545
                     /gene="dcm"
                     /locus_tag="ACG8NF_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            14535..14825
                     /locus_tag="ACG8NF_000947"
     CDS             14535..14825
                     /locus_tag="ACG8NF_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471446.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            15084..16268
                     /gene="mobT"
                     /locus_tag="ACG8NF_000948"
     CDS             15084..16268
                     /gene="mobT"
                     /locus_tag="ACG8NF_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0471447.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            16265..16402
                     /locus_tag="ACG8NF_000949"
     CDS             16265..16402
                     /locus_tag="ACG8NF_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0471448.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            17148..19058
                     /gene="ltrA"
                     /locus_tag="ACG8NF_000950"
     CDS             17148..19058
                     /gene="ltrA"
                     /locus_tag="ACG8NF_000950"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0471449.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            19162..19386
                     /locus_tag="ACG8NF_000951"
     CDS             19162..19386
                     /locus_tag="ACG8NF_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471450.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            19399..19902
                     /locus_tag="ACG8NF_000952"
     CDS             19399..19902
                     /locus_tag="ACG8NF_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0471451.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            19962..20351
                     /locus_tag="ACG8NF_000953"
     CDS             19962..20351
                     /locus_tag="ACG8NF_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0471452.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            20338..22785
                     /locus_tag="ACG8NF_000954"
     CDS             20338..22785
                     /locus_tag="ACG8NF_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0471453.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            22790..24913
                     /locus_tag="ACG8NF_000955"
     CDS             22790..24913
                     /locus_tag="ACG8NF_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0471454.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            24910..25914
                     /locus_tag="ACG8NF_000956"
     CDS             24910..25914
                     /locus_tag="ACG8NF_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0471455.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            25933..26844
                     /locus_tag="ACG8NF_000957"
     CDS             25933..26844
                     /locus_tag="ACG8NF_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0471456.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            27031..27287
                     /locus_tag="ACG8NF_000958"
                     /pseudo
     CDS             27031..27287
                     /locus_tag="ACG8NF_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27504..27719)
                     /locus_tag="ACG8NF_000959"
     CDS             complement(27504..27719)
                     /locus_tag="ACG8NF_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471457.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(27968..28186)
                     /locus_tag="ACG8NF_000960"
                     /pseudo
     CDS             complement(27968..28186)
                     /locus_tag="ACG8NF_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(28481..29920)
                     /locus_tag="ACG8NF_000961"
     CDS             complement(28481..29920)
                     /locus_tag="ACG8NF_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0471458.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT         9830 a         5417 c         6190 g         8872 t
ORIGIN      
        1 taataactca tgattcgaag cgatttcatt gaatgtttca taaattttat tcaattcagg
       61 ttttactttt tcattaagtg caaaaaagaa aacagaatga acaagtgtcc catcccaatc
      121 aattccttct ggattaacgt agacacagat tactggcttt tttataaaat ctacttcgat
      181 atgcggcata gcaaaatcat tcatcgctgt tgaagatagt ttttctcttt tgattgctcc
      241 ctctagtatc ccttctttcg cgtaattttc ttttaccaat tttttggtaa ttatatttaa
      301 aacgttcact tgattatcct ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa
      361 atttagaaaa gcatcttctg tttgttcatt taactgtaga tactgtttta cttttctgac
      421 atctgcccct aacatcaaag gactaacaac aataacagga aaatttgtat tttcaatcgg
      481 aatcgtgctt ataacaagtt catcagtgac ttttctatct tgtagttcat tgatgcttag
      541 tacatcttct atctcaacca tatctgcaaa accgttattt attctttgct ctaataattt
      601 tgatgttcca tatccgctac tacaaactaa tatcactttc gttttgtcag gtttgtaacg
      661 atccaaaagt gattgaatat ggagtgttac gtaagcgatt tcatcttcat taaaatgtat
      721 acagaatctc tcttcgattt cctctaataa atctacactt atcataaatg accttttaaa
      781 agaatatttg attttgtccg tataagggtt atagatattt actcccaatt tgagtctttt
      841 aactgctgca ttgagatgta atactagatt attgatcaat tcttcatcag gataaaattc
      901 atttaattta attcttacta actcttgtag ctcaacaccc atatctaaat aggaaagctt
      961 cgtttgatct aagtcgtgat tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac
     1021 ataagcttgt tcatctattg gcacaataat tgaaaatgct ttttcaatca ttttgattaa
     1081 aataagtgta ttctttgaga tttcttcttc agaattatta ctacgtgata agaaattgct
     1141 tgtttcaatc ctttctatcg caatagcaag gtggatagct aaagtttgaa attcataatc
     1201 tgtaaatttc aattgtgtct tattcatgta tcgattaagc aattcggcta cttttttcaa
     1261 tatgtcctta tttaagaaat tattcgcttc accaatgaaa ttgatattca agtctaagag
     1321 atctgtttta ctgttagatg ctgtaaagcc accccaataa tagttcacaa cttctgacat
     1381 caaccttctt ttactttttt catcagcttt gatgcgcacg ccatttcgat ctgaaactaa
     1441 ggacatccca tgcttgttta gtctttttct aacatctttc attgtgcttt ctaccgtcga
     1501 cctgctcacg tataattcgt cagcaagatt ttgaatcttt agagaatcat tagtattcaa
     1561 taatttagca aacacataag ttagacggtc ctccggagag tctgcgatat tgattttatg
     1621 actggataat aaatttcgaa gtgtttcttt ttttccaata agcgaaacgc cttttttagg
     1681 ttgaaccaac atttgtacat ctggcatttt ttggattatt tctttcaaaa ttaaaatatc
     1741 tttgctaatt gttttcgtag atacatataa ttgttctgat aattctttgt aagttgtgtg
     1801 ctcattttca atcaaaaagt atagtatttt catttgccgt tcgctaaatt cgatgcccat
     1861 tatcttcacc tcctgaatac agcttaagtg tacaattatt atctctattt tcatacagaa
     1921 gaaagttctt gattaatgta gaagtttcta atattcttga ttatttcaaa tcaataaaaa
     1981 agcctgcaat atttatatca caggcttttt tctctattaa atatatataa gataatacat
     2041 ttttctacta gttctcacta atcttgtata attaaaccct ccgcaacttg acggagatgg
     2101 agaagatgcc cccgtctaat ctagcggtca tctctcctcc cattccttcc accaattttt
     2161 ttgacaggta aagcccgatt ccgctagatt ccgtatttgt gcggctggta ttttcggtat
     2221 aaaaacgctg gaagactttt tcaacagagg tttgcgttcc tggtttgata ggattggcca
     2281 ctgttaatcg aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat
     2341 aacgcaataa attctgtatg atattttgca gcactcgcag caacatgagc ggatctgcta
     2401 atacagttgt atttatcgag tgttcagcga acgctacctc gatatttttc tcagaaaatt
     2461 gttcataaaa agagaaaaat acttcttcca cctctttaga aaaagaaagc gtatggagat
     2521 ccatttcttt ttgcttcgtc tctattcgag caagatcata aaactgatcc gtcatactga
     2581 gtaatcttgc aacagattgt ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct
     2641 ctacctgttc cagtaattgt acataaccgt taatagcagt caaaggagtt ctgagatcat
     2701 gagaaaggcc gacgatcgat agtctgatgt tttcttccat ttcttgaaat gcttgtcctt
     2761 tttgttcatt ttcatccaac catgtattca gtgcatctac taggtccatt agattctttt
     2821 ctcgaaattc gactgaaaga cgaccaccat attcttgtcg ttcagaaaga gtggcgattt
     2881 tcttagttaa tctcttgatt tccctttgca ggaagaaaat aaaacttccc ataaagataa
     2941 caacgagaag caacagccat ttaatcaaat ctaaacccca ctccccacac cgtatgaata
     3001 tacacagcat gtgcctttgc tagcttccct cgaagattgg aaatatgcat attcactgtt
     3061 ttctcactat caaaatatgg ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat
     3121 acttttttcg gatgttctaa aaaaagcttc agcagatcga attctcttcc tgtcaaatgg
     3181 acagccacac cattcacatt tacatccctc gactctttgt ccagtaagat ttccttgtaa
     3241 tagatgcgtt gcgacagatc ttttacttgg acagaagtct gatgacgaag ttggatgccg
     3301 attcgtaaca aaagttcttc gatatcaaat ggtttcgtca catagtcatc cgctcctgct
     3361 gccaacaagg aaactttatc tctttgatcg ctttttgctg aaagaacgat gatcggaaca
     3421 ttcgatgtct gtcggatttc tgctaacaat gcttcacctg aaagccctgg aagcatgaga
     3481 tctagaagga tcaaatcgac aggatatgtg cttaacaata attttccttc cgtacctgaa
     3541 taagcatcaa gaagtttgta tctctgttca agaacctcct ccagtaagga atgaacagct
     3601 tcatcgtctt cgataatcaa aatagtttcc agcaggacca cctacttaat gtcagatttt
     3661 tggaaaataa acattcctag agctgatgat accacgattg ttactagact gaccagaatc
     3721 attttcggtg cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt
     3781 tccgtaaacc actccgttag aaattccaag cctttgattt ttgcgaaaac accgggaatg
     3841 aaggccgttc ccatgatata aagaaaagaa agaagcaaac tgacactggt acttctagtg
     3901 atgaatgtca aagccactgg aacagataat gtccctaatg tcagcagaag caatacagga
     3961 aatactttta gaacataatt tccatagttt ttgattccct caacttcacc aaatccgaaa
     4021 gcaagtgtgt aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag tgttgtgatc
     4081 aaaagcgtaa cgagaagttt cgacacataa taagtcattc gattccgtcc taaagataac
     4141 gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc aaatgctgcg
     4201 aaagccagaa gaatccatgc agccattttt tctaccatta aaatccctgt tgcaccgttg
     4261 atgactgtat ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat ataccattcg
     4321 ttccaagaat aaaaaagcgg aagtacgacc atcagtccta gtgagaccca cattttacgg
     4381 ctcttccata cttttatttt ttcagcagtt aatagattaa acatgtggat tgcctccagt
     4441 taattgtaag aaataatctt ccagtttttg cccaactagg tgaataccat ccacaggaac
     4501 ttttgcttct accaatgctt gattgatggc tgctacttgg tcaattcctt caaaaagctg
     4561 gataacacgg ctagattgta caaaataatc cctataacca agagagtcga gaaccgttac
     4621 tgcttgttcc gtcgccgaag tgtcaatttt tataaattga cgagattctt gtgccaattc
     4681 ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa ataccatagt gagtggcgat
     4741 ctgtgacaat tcatctagca agtggctaga aagcaagacg gtgatgcccc gttcttttac
     4801 caaacgttgg atgatttctc gcatttctgc catccctgct ggatccatcc cattcgtcgg
     4861 ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca tttgcaatag ctaaacgctg
     4921 gcgcatacct aaagagaaat tctttgcttt ctttttccct gtattttcca atcgcatcat
     4981 tttcagcagg tcttcgattt ctctatccga cacaccgccg ttcgctgctt ggatacgtag
     5041 gttatctctt gcagttaatt ctggataaag agcaggggtt tcaatcgatt gacccatacg
     5101 tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt catcaatcga
     5161 gatcaaaccc ataatagttc tcataaaggt agacttaccc gctccatttt ccccaatcaa
     5221 tccatagatc attccacgtt tgatttcgat tgatacttta tctaatgctt tggcagcacc
     5281 gtattttttg gttataccag ttgctttcaa gaccgtttca tttccaaaat ccatacttat
     5341 ccttctttct tattgatgag tgaagtatag ttgacgggta taaagaaagc agccagaaat
     5401 gtataaagaa agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg acaatacgtg
     5461 tctttcctat tatcaggcag tttgttatac gcttgtatca gtttcctgat tctttctttt
     5521 cgaccaaagt cgtttctctg tattcctctt cactgaaaat ttttgcttct ttcagatcaa
     5581 gcttgagtaa ttttaccaga ccggttataa ggataactat agcaagactc cctcccagaa
     5641 aaattagact gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc gcaccaaaca
     5701 gtgctaatcc aagtggactg gccaccatgg taatcgttgt tatcgttgac ataagtcgtc
     5761 cttgtatatg cgttggaact tctcgctgaa gccagaccat ataaaaagca aaagaaaaca
     5821 ttaatcctta aaaacggact tatgaaaaat aatacatgcc gtttgggatt tacaattagc
     5881 taccaaataa gaggttatga caaaagactt ttcacaatcc cttcttccga ataaacggtg
     5941 ttctcctgcc aactcctcgg tcacaagtca caatatttat caaacatgag aagcaatttt
     6001 tgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca gaagcggata
     6061 aataatattg tttgatgata ctttatcttt ttcccgtttg tttatagatc gaatgatatt
     6121 atagaccaag aatcctacct attaacatag ctaaaaacac aaaaaaggca ctttccaaat
     6181 tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg ctcagcattg cttcgcccag
     6241 tacgattctc gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg
     6301 aagctttacg ggctttctta agacctggtt ttttcttcgt atcattgaat aagtttgaaa
     6361 aaccttgatt tataaaggtt ttcccgtaaa tatattttat tatatttgat taaatttgaa
     6421 atgtttgtgt tctaaaatgt gttctaattt aaaggcagta tatttcgtat tttagattaa
     6481 atttttaggc tcattcattc tcaaatccat tctattcagt caataaaaat tttaaaaaga
     6541 ctttataaac aaagatgttc aagcgagcat cttatttttt tgtgcttaat ttgaaaggag
     6601 aacatttcat gtataaaaaa caaaaaaaga aattatgtgt attaactatt ttgagtttgg
     6661 ctgttcaagc aattgttgga gcaattgctc ctacgattac ttttgctgat gaaattaccc
     6721 atccacaaac ggtaaccgta gaactagatt tagcacatca atatgctgtt gaaggaactt
     6781 ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg tacaacggct
     6841 ttaaacaaga tattttctgt attgaaccag gagtcccgat ttacaatgag ttcactcctg
     6901 gctatgagaa aaacccgtta ccagatatgt ccgataaagc caaattagtt tccgttttat
     6961 ggaaaaaagt aggtacagat gcagatactc atattgtggc acagaagatg atctggcaag
     7021 aagttaatgg gtataccctt cactcaataa aacgatcaga tggtagtgcc gtaaatatcg
     7081 cagccattga ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa ccaaccttcc
     7141 ataatagtac ggctaaaaca gtattagggc aatcgactac tgtgacggat acgaataatc
     7201 tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc aaacattgat tatcgtgtga
     7261 atggtaatca gttgataatc accccaaatg ccaattcaaa agaaagtggt gtgctgacgc
     7321 ttaagaaatc tgctggtacc ggaacacctg tcgcctataa aaaggctggc caacagactt
     7381 taatggccgg ggcgattgat aaacctaata cctacacaat caaaattgat gtggaaacgg
     7441 agggttcttt aaagatcaaa aaagtcgata aagaatcggg tgatgttgta ccaggaacgg
     7501 ttttccattt agactttgga aaaactttac ctgcaaaaga cgtgactact gacaaagaag
     7561 gcattgctac attggatgag attcctcatg gtacaaaagt aaccattacc gaaaaatcag
     7621 taccggcgcc ttatacgatt gataccaccc ccatgactac caccattaaa gcaggcgaaa
     7681 ccatttacgt gacttcaaaa aacgcacggg aaaaagggca aatcattctg gataaaagcg
     7741 gagttgaaac agggagtgat ctttggaatg acaattattc cttagctggt aatacgtttg
     7801 ccattcgcaa agacagtcca accggtgaaa tcgttcaaga aatgaccacg gatgaaaacg
     7861 gtcatgcgga aacaccaaaa gagattgcta atgcgttgga attgggaacc tattatgtga
     7921 ccgaaactaa agctagtcat gggttcgtga atacgttcaa acctgtaaaa gtcgaattga
     7981 agtatgccaa tcaaaccgta gcccctgtta ccagtaacgt aaaagggcaa aatcaagaag
     8041 tcactggcga aaccactttg acgaaagaag acaaagatac tggggataaa gcacaaggta
     8101 aggcagtctt tgagggtgct gaatacacct tgtttactgc taaggatgga aaagctgtta
     8161 aatggagtga ggcttttaaa ccagagttag cgaagggaac gaaaggttct gatgaaacgg
     8221 tcactttgac tttagacgaa aagaaccaag ttgccgttaa acatttagct atcaacgagt
     8281 attattggca agaaacaaaa gcgcccgaag gatatagcct tgatgaaaca aagtatcctg
     8341 tatccatcaa aaaggtggat gatagcgaaa aaaatgccgt gattacccga aatgttacgg
     8401 caaaagaaca agtgattcgc tttggctttg atttctttaa atttgctgga tcagctgcgg
     8461 gcactaccga aactggcttc aatgatctga cctttaaagt gtcgccattg gaagggacca
     8521 atgaaatcac aggtgccgaa gatgaagcga ccacagctta taacgagcaa ttagggtttg
     8581 atggttatgg caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagaag
     8641 ccccagaagg ttttcaaaag attacgccat tagaaatccg ttctactttt aaggaaaaca
     8701 aagaagactt tgtgaagagt gagtatgtct ttaccattac cgaacaagac caaaaacaac
     8761 caattaaaac ggtgaccgtt ccttacgaga agctgacgaa caaagcattt tctgttagtt
     8821 tgaaccgttt aatgctctat gatttgcctg aggaagaaga tagtttaact tctcttgcga
     8881 cttggaaaga cggaaacaaa gaattaactt ctctggattc taccgagcta gttgataaat
     8941 tgagttacaa tctacatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg
     9001 tggatgcgac aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtgattgcag
     9061 aaacaagcgc aacgttggcg aacaaagaga aaacaggaac ttggaaaatt cagcataaat
     9121 taaccgccga acaagtttta aataaaacta ttgtcttatt caactatgtg tatgaaaata
     9181 aggaagcttt tgaagcaggc gataagccag tagcgaagga tgttagcttg aataaccaag
     9241 cccaaactgt cagttgtaca gtggaacacc atgtttctat ccaaacaaag gcccacctag
     9301 aaaacggttc tcagaccttt actcatggtg atgtggtgga tatgtttgat gatgtgtcga
     9361 ttactcatga tgtactggat ggctcaaaag aagctttcga aacaattctg tatgctttat
     9421 taccggatgg tacaaacaaa gaaatttgga aatctggcaa gattgattat gaagtaaatg
     9481 ataaagaatt caccaaaact gtgcttgcga aaaaagtaga taccggaaaa tatccagaag
     9541 gaaccaagtt tacttttgcg gaaatcaatt acgataaaga tgggaatatc aatggaaaac
     9601 acaatgaaga tttgaaagaa aaatctcaaa ccttaacacc aaaagaagtt ccaaccatac
     9661 taagtacgcc gaaacaaccg gaaacaccaa ctgttccaag tggctctcaa gaatctagtc
     9721 ccacagtgaa gaccttccct caaaccgggg agaaaaattc caatgtttta ctgttcattg
     9781 gttttacttt gatttttgcg acggcgggct attatttctg gaatcgccgg aactaaggtg
     9841 atgagatgat acaagtaaat cctaagaacc aacgtgcgcg accgcctcct aaaaaagtag
     9901 tcgcgctatt tttgtatcca aaactaacaa tgaaaagagg aaaagaagat ggaattgaaa
     9961 tttgtcgtgc cagatatggc tgaaaccttt ggaaagttaa gttatgccgg tgaaggagaa
    10021 attataacag aaggatatgg acggaatact acggtgattg gtcgcagtta tcatttgtat
    10081 tccagtaagc aacgggccga tgatattgag gtggtagtag ctgcggaagc cggtgaaaag
    10141 gattttgatc aagaccaacc gctaaaagcc gtgaatcccc atttggttgc caaaggctat
    10201 gagattgaga atcgtgggtt tactgattac gtgttgtatg ttgatgattt agtgaaagca
    10261 taggaggaaa agaagatgag attaacagaa ggtattgtcg tagattcagg tttaacattt
    10321 gggaagttgc gtttttccgc attacgccga gaagtacgga agcaaaatga ggatggaaca
    10381 attggtaatg aggtaaaaga acgaacctat aatttaaaat cctctgcaca agggcgaatg
    10441 attcaagtta gtatcccagc taatgtaccc ttacgcgagt ttgcttatga tgcagaggta
    10501 gagttggtca atccaatcgt ggataccgtt gccaattatg tttttcgaga aggaactacc
    10561 gttaattggt tcattaaggc ggatgattta gtcttgaagc ggcagccgaa tcaaggaaat
    10621 cctacgaacc aaaatgaaag aaagaaatag gtgaagatta tggaaattaa tgttgaacat
    10681 attttagatt gtctcgatca atatggaaaa ggcgagctga ctgaggaaca actcgttaaa
    10741 gcattaacct ctgatgaaaa aatgtttctg attatacatc aaggtttact tggagaaaat
    10801 agcgacagtg aagaagactc tgatgtacta gactggttag gagaagaaga gtctttcttt
    10861 atagttgtcg aagtaaatga aaacttatgt cggcaggcag aatccatttt ggaagaaatt
    10921 ggtgtggaga tgccggatgc tattgaaggc tttttaaatc aattggttga aacgaaacaa
    10981 ctcccctcct cgtagcagta aatgactagc ataacttttt gaaattgcct aatgaaatcc
    11041 tattgggctt tttcgattgg aggtggaaac catcaaaatg tacaaaggac atcgcatacg
    11101 cgcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat
    11161 cggttggatg agcgtctttt attttcaaga gtttagacaa tttgatattt ctaagctatc
    11221 actttctact attgaaatcg tttggtctat aaaagattta gtatgtctgc ttggaagtct
    11281 cgctttttca ggagctatga tattgcttta tattcacttt tttcttgatc attggcgaag
    11341 cttgtggcat cgacaaaagc tagctcggat gattctagag aaccattggt atgaagtgaa
    11401 acaaactcaa agtgaaggct ttttcaaaga tttgaatagt agccgaacca gagagactat
    11461 cagttacttc cccaaaatct gttatcgaat gaaagatgga ttactgtcta ttcgcgttca
    11521 aatttcactt gggaaatacc aagatcagct tttaaaattg gaaaagaagt tagaaagcgg
    11581 actgtattgt gagttagtag aaaaggaact caaagactcc tatgtggagt atactttact
    11641 gtatgatatg atcgccaatc gaattgggat agacgaagta gtggcagaaa atgggactct
    11701 gcgattgatg aaaaatcaag tatgggctta tgattcttta ccccatatgt taatcgctgg
    11761 tggcacgggt ggagggaaga cctatttcct cctcaccatt atcgaagcac tattgaagtc
    11821 agatgcagaa ttgtttatcc tcgatcccaa aaatgcagat ttagctgatt taggtacggt
    11881 gatgcctcat gtttattcac aaaaggaaga aatttctgct tgtgtggaag atttctatga
    11941 acgtatgatg gctcgtagca aggcaatgaa agaaatgtct aactacaaaa caggagagaa
    12001 ttatgcctat cttggacttc cacccaactt tttaatcttt gatgaatacg tggcttatat
    12061 ggagatgtta acgacgaaag aaagtgcggt gattttgaat aagctcaaac aaatcgtgat
    12121 gttgggtcgt caatctggtt tctttctgat tttggcttgc caaaggccgg atgcgaaata
    12181 tttaggagac ggaatccgcg atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga
    12241 actcggatat tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa
    12301 aggtcgaggt tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt
    12361 accaaaaggg tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt
    12421 gatcttcaat actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga
    12481 aaggggataa ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag
    12541 gataagttta tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt
    12601 tttatggcag ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat
    12661 tatgataacc atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat
    12721 ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga
    12781 tatgtcttag atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct
    12841 gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat
    12901 caagacccta aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat
    12961 gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta
    13021 aagagtaaac atccagatgt attgcttcat atcataataa aagattatac gaataatatt
    13081 tccgaagttc attatgctac ggataatcta gaattatttt taccactagg gtatagtagg
    13141 aaagatttgg aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa
    13201 atacaataag agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt
    13261 gattaaatat gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa
    13321 aaaatagtag gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc
    13381 tttgcaatga atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt
    13441 tcgtttaggt atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt
    13501 tgctagacgt agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat
    13561 cacaagtgta tcagatgagt ttattcaatc cctcggacca gtggacatcc tttgcggcgg
    13621 atttccgtgc caagcttttt caattgcggg aaagcggcaa agattttccg atactcgagg
    13681 tactttattc tttgaaatcg ctcggttcac cactcttctc cgacctaagc ttttattcct
    13741 tgagaacgtc cgaggattgc tcaatcacga aggaggggct acgttcgaga cgatcctccg
    13801 aacgctggat ggattggggt atgacgtgga gtggtaggtg cttaactcaa aggcctacat
    13861 tccccaaagc cgggagcgga tattccttat cggacattct cgagacgcat gtaccgaaca
    13921 agtatttcct atcattggac cgtcttcaac atctgatcaa aacatcagaa atttggtaaa
    13981 tatcaatcct tccaatcgag gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc
    14041 aaccttaaca ggtgatgaag gaatcaaaat cgcactgcca gcaagggatg gaatctctgt
    14101 agcaggacta ttaccgggaa agtttgaaca aggtaatcga gtttatgaag ttactggaac
    14161 cgcccccacc ttgtcaacga aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac
    14221 agaatatcaa gaggtgaagc cagaagacag tgtgaatctg gcttttccta attccacaag
    14281 tcgccgaggt cgtctgggaa aacagagtgt tcatacctta ctaacaggag accaacaagc
    14341 gatcgtcacg gatcagtatc aaattcggaa gctaacacct agagagtgtt ggcgacttca
    14401 gggctttccc gattgggctt ttgatcgaga ttctcaagtc aattcagata gtcagttgta
    14461 taagcaagcc ggaaactctg taacagttcc ggtcattttt gatattgcca gaagattaaa
    14521 agagttggat gtacatggtt tatgatgagt tgattggaga aatttattgg gtgattggaa
    14581 agattcagtc agacccagaa ttagaagaac aattgcgacg gttaaaattt gatattcgca
    14641 agaatggtgt caaagtcgct ggcgatccat atttaatgaa tgaggagacg gatgctcgaa
    14701 ttgaaataaa tcaagtaatt gcagaatttg agcggattgc ggaccaagca aaagaaccag
    14761 atattcgtca atacttgtta gaaatgaaag cagagttgga agtaaatggt attacagatg
    14821 agtaacctaa accaacacaa ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag
    14881 aaatcctccg tggtgctgtg cctgttcatg gacggcgtta gccgaccatg atgtctcacc
    14941 cccgtatcta acaggggggt acaaaaacac aaaaaataag taagcaacca ttaagaatgc
    15001 ttgttgtatc aaagatagta agtaatttgt ataggtgtta actcatttga aaaagttaac
    15061 accttgaatt tggaggtgag aacttggcac aacggaattt agattatcgg ctattaaaag
    15121 accgacgcaa cgaatacggc gtttcacaaa ataaattggc tacggcttgt ggacttagtc
    15181 ggccgtactt aaatcagata gaaaatggtg gcgtaaccgc atctataaaa accatgagga
    15241 aaatttttga ccaactggaa agctttaatc ccgatttgcc tttgacgtta ctgtttgact
    15301 atgtaaggat tcgctttccc acaacggatg cacgaaaaat tattcaagaa attctccatt
    15361 tgaaatttga gtatatgctc catgaagatt atgcctttta ctcctatcaa gaacagtatg
    15421 tcatgggtga tattgtggtg atgttgtctc atgaagaaga taaaggtgtg cttttagaat
    15481 taaaggggcg aggttgccga caatttgaaa cttttttact cgctcaaaag cgtagctggt
    15541 acgacttttt cgaggattgt ctaaaggccg gtggcgtgat gaaacgcttg gacttggcaa
    15601 tcaatgatcg agtggggcta ttggatatac ctgagctaac gaaaaaatgt cagaaggaag
    15661 aatgtatctc cttgtttcgt acctttaaaa gttatcgttc cggggaactt ttaaaggctg
    15721 atgaaaagga cggcatgggc aataccttat atatcggcag tctcaagagt gaggtttatt
    15781 tctgtttgta tgaaaaagac tatgaacaat atatcaagtt agggattcca ttagaccaaa
    15841 ctgagacgaa aaatcgtttt gagattcgac tgaaaaatga ccgtgctttt catgcgattc
    15901 aagatttgtt aaaaggtcgt agcattgaga gcaccacgtt ttccattatc aatcgttact
    15961 tgcgatttgc ggataaagta gaaggtaaaa ggcgaaccaa ctggcccttg aatgaacaat
    16021 gggggcgttt tattggacgg aatcggaaag agattcaatt aacttccgag ccaaaaccgt
    16081 ataccattga acgaaccttg aattggctag gccgacaagt cgcccctacg tggaaaatgg
    16141 ctaaggagtt ggatcgattg aatcggacaa cctatatcca ggatatggta cagaatgcac
    16201 gcttatcaga ccgacataaa aagattttgg agcaacaaag tatggcaatt gaaaatttga
    16261 ttatatgaaa agaggaatcg tcatgggaat tgtaaaagat atttttttgg tatttggtgg
    16321 cagtatgctt ggtgttactg tgatgtgctt gatgaatgca agtgcagcag cggatcgagc
    16381 aatggaaaag aaaggaaagt gacaagatga atttcggaca aaatttatgt gcgactcgtt
    16441 ccttagtgaa aagctaaggc actaataaaa gaacgaaaaa gtttggttat taggagaact
    16501 gataatctga caggtggaat gatgaagtaa cgctctgaaa cgcctgccct gagcctccga
    16561 caagcattgt atgaggaaac tcagaatgtt tgaagctcgg taaagacggc tgaaagatac
    16621 cgtaatagtt gatgaacata tatcttctat cgaaaactat ccagaggtgg gtagcgtatc
    16681 caacaggtcg ggggtctata aaatatctat ggttagaatg ttctataagg aactgacgaa
    16741 acagcgaatg tacgggtcta aatttgaact ttgtagaaat gcaaaggtac gagaaatcgt
    16801 agctggtgtg gcaaagtaag aatataggtt atgaaatgcc atataacgtt acaggcggta
    16861 tcaagccagc aggcttatag ctagtgacct aaggatatat atgtatagat aagattatcg
    16921 gaacgaggaa aggtattggg ttctcatcaa gagaataaac tgacgaagaa caataagcag
    16981 ttgaactaat gctgaaaagc agagggcgaa cctaagaaat tcttgtaatg agaatggagg
    17041 aatgccctca agtcgtgtat atattagaaa agtattatgt tgtttcaata aggattacga
    17101 gtaagataaa aaagctcact caatttcaag taaaggagtg aagccttatg cctaaaaata
    17161 aaaatgaaac attgtgtgta gaagatttaa gacacgctga atattacgaa atgcaaaaca
    17221 cctttgatga tttatatgcc aaaagtaaga atggagatat tttcactcat cttatggata
    17281 ttatcctatc gagagaaaat atattactgg catatcgaaa tatcaaagca aatgcaggca
    17341 gtaaaacagc aggaacagac ggtacaataa ttaaagatat tggcaaattg ccagcagaaa
    17401 cagttgtcaa aaaagtaaga tacattgttg caggcagtcc acatgggtat cgacctaagc
    17461 cagtgaggcg aaaagaaata ccaaaaccaa atggtaaaac cagacctcta ggtattcctt
    17521 gtatgtggga caggcttata caacaatgta taaaacaagt attagaacca atctgcgaag
    17581 ctaaatttag tgaaaacagc tatggtttta gacctaatag gtcagttgaa aatgcaataa
    17641 aagcaaccta taaccgccta caaatcagcc agctacatta tgtgattgaa tttgatatta
    17701 agggtttctt tgacaacgtg aaccattcca agctgataaa acagatatgg gctatgggaa
    17761 tacgggacaa gcacttgata ttcattttaa aaaggatatt aaaagctcca atcaaaatga
    17821 ctaacggtac aataacatat ccagagaaag gaactccgca agggggaata atatccccac
    17881 tactagcaaa cattgttctt aatgagttag accattgggt agaaagccaa tggcaggaaa
    17941 atcctgttac aaaaaactat gtggttcata tcaataaaag cggtagccca tgtaaaagca
    18001 acgcttacaa agaaatgaag aaaacaaagt taaaggaaat gtatatggtt cgttatgcag
    18061 atgattttag agttttctgc cgctacaagg aaagtgctga aaaagccaaa atagcaatca
    18121 ctcaatggat tgaacaacga ttaaaattgg aagtatcaca agaaaaaaca agaattgtaa
    18181 atgttagaaa aagatactcc gactttcttg gtttcaagat taaaatgata ccgagaagaa
    18241 agaagttagt tgttaaatca catataagtg ataaacaatt caaaaaccaa aaagacaagc
    18301 tggtatcaca ggcaaagaaa attgcttctc cctcaaaagg gaaaacggaa ttagaagaaa
    18361 caagacttta taattcaatg gtattaggta tgcaaaatta ttatcagatt gcaacgaatg
    18421 taaatctgga ttgtatgaaa ctgaatagag ctgtaatggc tatcttcact aacagattag
    18481 gtacaaacaa aaggggcaga ttgagaaaaa caggcagaca acttacgaca aaggaggcaa
    18541 caagatatgg taaatctaaa atgcttcggt atgttgctgg cgagggcgaa cctattttcc
    18601 ctgttggata tatccaacac agaaaaccaa tggcaaaggt gtatttagcg aattgctaca
    18661 ctctagaggg aagaaaattt atccacacca atctaagtgt tgataaatta ctgatgtacc
    18721 agttaatgaa attatctttg gaaaatcgca caatagaata cgcagacaat cgaatttcgc
    18781 tattttctgc tcaacatgga aaatgtgcta taactctgga agaatttcaa caagctaatg
    18841 aaatacattg tcatcatatt gtaccaactg gagctggtgg aaatgacgat tataaaaatc
    18901 ttattttggt aaaagaggcg gtacaccgtc taatacacgc taaaacagaa gaaacaatac
    18961 aaaaatatat agctctattg aagctaaata aagtccagct cataagacta aataaatata
    19021 gagtattagc tggcaatcaa gaattaaact tgatataggt ctactacata aatacttttc
    19081 aatataaaca cgatggaacg cggagtgcgg tgaaagtcgc acgctccgtg tgaatggggg
    19141 gaaaagtttg agatatttat aatatcagga acttacctat ccatatatca atggttttta
    19201 accaatgccc aatccttagt cttactggca attgtggtca ttggtttgtt tttaggattt
    19261 aagcgggagt tttccaaact gattggcttt ttggtgattg ctttgattgc ggtcggattg
    19321 gtctttaatg cttctggggt gaaggatgtc ttactcagtt tgtttaaccg aattgtcggt
    19381 gcgtaagggg gaagcacgat ggaacagatg cgtgtttata ttgcgaacct cgggaaatat
    19441 aacgaaggtg aattggtggg ggcgtggttt accccacctg tcgattttga tgaagtgaaa
    19501 gaacgaattg ggttaaatga cgaatacgag gaatatgcaa ttcatgatta tgaacttccc
    19561 tttgagattg atgagtatac gccaattgag gaaattaatc ggttgtgtgg cttagcggag
    19621 gaacttgaag gaacgccagt tggtgaagtt gcttctgaga ttcaacatgc tttcttcaat
    19681 tcttttgagg agatggtaga gcatgtggat gatattatct attatccaga ttgcgatgat
    19741 atgagtgatg tagcatattt cttgattgta gaagctggtg attttggaga agtaccagaa
    19801 cacttgaaga cgtatattga ttttgatgcg tgtggtagag acttggagat tggaggcaat
    19861 tatttggtaa cgagtcgagg tgtttttgag tatccatatt aacaaaatta ttgaagggta
    19921 gttgatttct aaactatcct tctttttaaa ggaatgattt gatgaagaaa ataaaaagct
    19981 acaccagtat ttgggcagtt gaaaaggtca tttatgcaat taatgacttt caactgcctt
    20041 ttcctgtgac ttttaaccag atggcttggt ttgttttatc cttgctgttt gtcatcgtat
    20101 ttgcccatgt gcctccacta tctatgatag agggggcctt tttaaaatac ttaggtatac
    20161 cggtggcagt tacttggttc atgtcgcaaa aaacgtttga cggtaagaaa ccattgggat
    20221 ttttacgatc ttttattagc tatcacttac gatttaaggt cactttcgca ggaaagaaag
    20281 tcaaagaaca gaagaaacga tgggacgaac ccattactct ggtaaggagt gtgaactatg
    20341 tacccgatta agtatattga aaataatctt gtctttaatc aagagggaga atgttttgcc
    20401 tactatgagt taatacctta caattactcc tttttgtcac ctgaacagaa atatcaagtc
    20461 cacgataact ttcgtcaatt gattgcgcaa aatcgtgaag gcaagattca tgccttacaa
    20521 attgccacag aaagtagtat tcgtgccacg caagaacgat caaaaaagga aattaccggg
    20581 cgattaacag aagtagcgaa acaacgaatt gatttacaga cagaggcatt agtatccatg
    20641 attggcgata gtcagattga ttatcgcttt tttattggct tcaaactgat tgcgacagat
    20701 gaagaggtca atctaaagaa tctcaagaaa tctttcttct ccggctttca agaatttgtc
    20761 tatggagtga atcatcattt gatgggcgat tttgtttcct tatccaatga agaaattcgc
    20821 cgatatacca agcttgaaaa actaatggaa agtaaattgg ctcgaagatt taaagtcaga
    20881 cgagtcacac cgagtgatct gatgtatttg attgaacata tctatggcga aaagggtaca
    20941 ccatttgaag aatatgaatt tcagctacca aagaaaaagc tgaagtcgga aactttggtg
    21001 aaacgttacg acttattacg tcccagtcgt tgtttgattg aagaaaagcc ccggtactta
    21061 tgcatggaac atgagaatca tgaatcatat gtagcgtact taacaattaa tacaattgtg
    21121 ggggaaatgg agtttccctc ctcagaactt ttctattacc agcaacaaca atttacattc
    21181 ccaattgaca ctagtatgaa tgtggagatt gtgacgaaca aaaaggcact tgctaccgtt
    21241 cgcaataaga aaaaggaatt aaaagacttg gacaaccatg cctatcaatc tgacaatgaa
    21301 acaaattcca atgtcttaga tgcattggat tccgtagatg aattggaaac taccttggat
    21361 cagtccaaag aatccatgta caaactgagc tatgtggttc gagtaagtgc cgaatcagta
    21421 gatgaactaa agcgccggtg tgatgaagtg ctagattttt atgatgatac caatgtaaaa
    21481 ctagtccggc cttttggcga tatgatgggg ctacatgaag aattcttacc ttctagtaag
    21541 cgatacatga atgattatat ccagtatgtt acgtctgatt ttctcgccgg acttggtttt
    21601 ggcgccactc agatgttagg ggagttggaa ggcatttact ttggttataa cgtagatacg
    21661 gggcgcaatg tttacctgaa accggcattg gcctcacaag gggtcaagga ttcagttacc
    21721 aatgcgctag cagcggcatt cctcggttca ctcggtggcg gaaagtcctt tagtaacaat
    21781 ctcttagttt attacgccgt cctttttggt ggacaagctg tgattgtcga tccaaaaggg
    21841 gaacggggtg gctggaaaga aaccttgcca gaaattgcgg atgaaatcaa tattctcaac
    21901 ttaactagta agccaaaaaa tcaaggatta cttgatcctt atgtgattat ggagaaaaag
    21961 aaggattctg aaagtctagc aattgatatt ctgacctttt taacaggaat ttctagtcga
    22021 gatggggaga agtttccagt gttgcgtaag gccattcgga cggttaccca aagcaatcaa
    22081 cggggattat tacaagtaat taatgagctt cgaaaagaag gctcaccagt tgcggaaaat
    22141 atcgcggatc acatcgagtc ctttaccgat tatgattttg cccatttgct tttctcagat
    22201 ggttcgatta cccagtcgat tagtttagat aggcagttaa atatcattca agtagcggat
    22261 ttggttttac cagatgcaga aaccactttt gaagaatata ccaccatgga attgcttagt
    22321 gtttccatgc taatcgtcat ttccactttt gccttggact ttatccatag taatcgagaa
    22381 atcttcaaaa ttgtagactt agatgaggcg tggagttttc ttcaagtagc gcaagggaaa
    22441 acattatcga ataagctcgt tcgtgctggg cgggctatga atgccggggt ttactttgtc
    22501 acgcaaaata gtgatgattt actggatgaa cgcttgaaga ataatatcgg gttgaagttt
    22561 gcgtttcgca gtaccgatat tcatgaaatc aagaaaacct tggaattctt tggcttggat
    22621 cgagaagatg aaagcaatca aaaacgccta cgagatttgg aaaatggcca atgcttgatg
    22681 caggatttgt atggtcgggt aggcgtggtt caggtacatc cagtctttga agaactcttc
    22741 catgcctttg atacccgtcc acctgttcaa gagagaatgg agtgagggga tgaaaaagaa
    22801 actttttcgg attgtgggaa tcgtttttat tagcatactc actctgatag ttctattgag
    22861 tttagttggt acggtagccg aggcgacagg gctagttgat gatacagttg aagcaggaaa
    22921 tctatattcc aaatattcac tgaacaacta tcaactagat ttctttgtgg atagctcttg
    22981 ggattggttg ccttggaatt ggggtgatgg cttagggaaa agcgtgatgt atggtcttta
    23041 tgccattact aatttcattt ggacggtgag tttgtatttg tcgaacgcca cgggttatgt
    23101 ggttcaagag gcttacaaat tagatttcat ttcggatacg gctgaaagta ttggaaagaa
    23161 tatccaaaca ttggctggta ttactgaaaa tggactccag acctccggat tttattttgg
    23221 gtttctatta ttgatgattt tagctctggg ggtgtatgtg gcgtataccg gattattaaa
    23281 acgagaaacc acgaaagcag ttcgagccgt gattaatttt gtgatgattt ttctactatc
    23341 gggttcgttc attgcttatg cgcctaccta tatcacaaaa atcaatgact ttagctcaga
    23401 tgttagtgaa gcggccctta cccttggaac cgagatcgtt gtacccaatt cagaaagcca
    23461 aggaaaagat agtgtggatt tgattcggga tagtttgttt tcgattcaag tccaacaacc
    23521 ttggttacta ctgcaatttg atgattccaa catagaagaa attggtgaag accgggtcaa
    23581 caagattcta tctgtgagtc cagatgaaaa caaaggcaaa gatcgggaag aagcggtcaa
    23641 ggcagagatt gaagacaatg acaatgcgaa tctcagtatt accaagacca tgaaccgttt
    23701 agggaccgtg gtctttttgg tattgttcaa tattggcatt tccttctttg tttttcttct
    23761 aacagggatt atgttgttct cgcaaattct cttcattatc tttgcgatgt ttctgccaat
    23821 tagtttctta ttgtccatgt tgccgactta tgaaagttta ggaaagaagg cgattattcg
    23881 tttattcaat acgattatga tgcgagcggg tgttactttg gtgattacga ctgcatttag
    23941 catctccacg atgtttttca atatttcggc aacctatcca tttttcatgg ttgcttttct
    24001 gcaaattgtg acctttgccg gtatctattt caaattgggc gacattatga gtatgttcaa
    24061 cctccagagt aatgatagcc agtcaatggg aagacgcgtc atgcgaaaac cgcaaatgtt
    24121 aatgaatcga aaattgcggc aactcaatcg aaatgtggga cgagctttgg cttttggtgg
    24181 tggtgcagca gtcggaaata agttggcaaa ggaacaatca aaatccaaat ttaagccatc
    24241 cggttcttct ctacgaaaga attcacgatt accgaacgat cacgaaatac tatctgactt
    24301 aactaaggag aatccagttt caaataataa gaaacaatcc cgaatgaatt tggctgggag
    24361 aaaaatcggc aaagttttgg atacccaagc tttggtgaaa gataagacta aacaagtaaa
    24421 ggatcaggta aggaatacgc caacgaattt gaaatacaat ctccataaag gaatcgaaaa
    24481 gtcgaaaaaa gcaccagaag aattcaagcg cggactgttc caagaaaaag cggatcgagt
    24541 ggatttgcga gaaaaacaac gacaacgaag aaatgcgaaa atggacgaaa aacgcaagat
    24601 actgagtgaa gcaacaaatc gtcagggaaa aagaagggcg aatattccaa tagataaagc
    24661 agctcaacct cagaaagata tgatgctgaa aaaagaagca ccaaagcgga ttgtgagagc
    24721 aaatctgaat cccgaaatca aacgaaaact aactagtcaa gaaatcattt caaaagggaa
    24781 aagccaatca gcaagtaaga aaaattcttt ccaacaagtg aaacagaggt ccactctgcc
    24841 aaagcagacc aatcaaaaag tacaaaaaag aatgaaccat cgtccaaaat caaggtcagg
    24901 tgagaaaaaa tgaacttgaa gaaagtgggt gttatttcat taacttttct gaccatctct
    24961 tttatgggta ttttgatgtg tgtgggtttg ttgtttggtg aagacagtga gggaaatggt
    25021 agttctggca cttctcctgg cggaactagt gtctcagaag aagtattgaa acaccgtgca
    25081 atggtggaaa agtatgccaa agaatcggat ataacagagt atattccaat tcttttggcg
    25141 attattcaag tggaatctgg tggcacaatg gaagacgtta tgcaaagttc tgagtcagcc
    25201 ggattaccac ctaattcatt aagtaccgag gcatccatca aacaaggctg tctctatttt
    25261 gcgtctttgg ttaagcgttc caaagaacta gggtgtgatc aagatagtat tattcaggcc
    25321 tataactatg gtggcgggta tctagatcat gtcgggaaaa acgggaagaa gcatagtttt
    25381 gctttagctg aatctttttc aaaggaaaaa tcaggtggtc aaaaagtcga ttaccccaac
    25441 ccaatcgcga ttaaggaaaa tggtgggtgg cggtataatt atggcaatat gttttactgt
    25501 ttattagtga agcaatacct aactacaact cagtttgatg acaaaatagt ccaagaaatt
    25561 tttgatgagg catttaagta tgaaggaacg gcctatgtgt ttggtggctc aagtccagaa
    25621 acggggtttg attgtagtgg cttgacccaa tggtgttatg ccaaagttga cctgaaactt
    25681 cctcgggtgg ctcaggatca atatgatgcc atgactcata ttgatctaaa agatgccaaa
    25741 ccaggtgatt tagttttctt tcattcaacc tatgatgccg gtacttatgt gacccatgtg
    25801 ggcatttacg caggggagaa tcgcatgttt catgcaggag atccagtggg gtggacgaat
    25861 ctaacggaaa actattggcg gcagcatctt atcggagctg gccgatacaa ataagagaaa
    25921 agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa aggaaaagag cccaaaaaag
    25981 aaaaaagaac gagttgtatc cgtgggtaag catcgaaaaa tggtgcttgc cctttggctt
    26041 ttattgtctt gtagtcttgc ttttggcgtt tataaaaatt tcaccgccat tgatcaacat
    26101 acaactcatg aaaaagtggt tataaaagag aaggtagtga atacttctgg cgttgaaagt
    26161 tttacagagg attttgcgaa agaatatttt tcatggaaaa ataacaaaga agcaattgaa
    26221 aagagaatga gtaatctcga acagtacctt acagaaaacg gtctcgcttt aatccaagat
    26281 atggttcgag cagatattcc cacgagttcc gaagttcaat ctgtaaaaat tctagatgtt
    26341 gaaaaacact cggaagaatt tgtcgtttca tttttagtgg agcaacatat tacagaggga
    26401 aaaaagacgc aatcaatttc ttctgcatat cgagtgacaa tctttgaaga tgaaaacagg
    26461 aatcatttag taactagttt gccaacaatg attggaaagc cagacaaggc aaaatatgaa
    26521 gcaaaacaag tagaaaacga ttcagaaatt gatgccaaaa caacggaaga aatcaccgat
    26581 tttttagaaa cattttttaa actttatcca tcagcttctg aaaaagaatt ggagtattat
    26641 gttgaaaatg gtgcgatgcg accaatcaat attgacttaa agttttcgga gttagagaat
    26701 ccagtttatc tacaaaacgg aaaaaatttt ctagtgagta caaagataaa ataccttgat
    26761 aatgtatcaa aaaccactag ctactttcag tacaaattag tattacaaaa aaatggaaac
    26821 tggaaaatcg ttgatgttaa ataataatga ctcaatctaa ttttattaga agttataagt
    26881 agtgtaactt atttcaggca gaagtcgccc atcctctata ggtgggtgat gaatgccatt
    26941 cggtaactcg aaaaccgaca attaagacat agtgcctttt tattttaatc catgtcataa
    27001 tcagtctata taggaaatgg ttgatatacc atggaattag atacaaataa tcattcagtt
    27061 tttcttcttg gttaccccct tattttagta gtgaaacatt gtaaacatgt cattgatgat
    27121 gtaatgtcag cttatgcaaa aactgccttt gaaagaattt cagaatcaca tcacattacc
    27181 ttagatgaat gaaatcgtga taaagaatac tttcatatta tgctcaaagc acaacctaaa
    27241 accgaattaa caaaattcag caatgcctaa gctcacgtct gataaaatga gactttctaa
    27301 gggtcaaaaa tttcctttga atacgattat tgcttagata aaagtaaaag ccttagaact
    27361 acggggatag cttggttaat aagagatatc gttgttagta aagacatagg cttgcaagta
    27421 tgctctattc ccaagaatct cccccttcaa gcaccaagga tcgttaaatt cagctaagtg
    27481 gcaagtaatt cactagacga taattatctc catatttcaa tctcagcttt tccatttttc
    27541 atagcaaatt ccgttatttt tatagcatct aaaagattca gcttttccac tgatctttca
    27601 ccacttttca gcttttttat tgtgcttaaa ttgataccag taccttttga tattgaattg
    27661 gaagtaaaat cattaagggt tttttttatt aaattcatat ccgcaattcc agcgttcata
    27721 agaatgaaga ttctcctctc atagactact ttgaaaatgg tactacagtg gctgtttata
    27781 tatttcagta ggttactgta gtggtcatct tattataatt agaaaaggaa cctaaatcaa
    27841 aaatgctaaa gaaaattttc atagtattaa gcctctcatg actaaagcca cggaattctt
    27901 gataaaagag tatacttgca agcctaaaca gcggtatctc ttattaacca agctatcccc
    27961 gttgttccta aggttcttga tgttttctaa ggcaatgctt gtattcatag cccttggtca
    28021 aaatattgat actatcgttg acgtttcgat tatgatgtat acggcaaata ggacaagtcc
    28081 attctcgaat tgtgagtgac ttcttaccat ctgtgtgtct acattctgaa ccaatttgac
    28141 tagacggaaa tcatttatct atcttaatga ttttttgaca gaagtatcga aaatctttac
    28201 ataatctggg tgatgtattc ttatgcagat gagagcgttg gctccgtatt cataaccagt
    28261 agcctctaaa acgtagtcca tcggtgggat acctagcttg cggttgccag cggctttcgc
    28321 agtcttcttg tgatcaaatc aagcatctca ttgagcacta gtattttgaa gataatactt
    28381 gagtttttcg gcttttagaa gttcacttca tcttccatca tccatggaaa tcccactcac
    28441 ccatacgcag gcctccaaaa attcttcaac agtggttttt tcagacattt ttctgaatac
    28501 ctccttgttt tttgatacgc cagatagtca ggaaaaaggc aacaaaacac aaaacaaagc
    28561 tgcctaaaaa ggtaatcttc attccatata aaaagacatc cggccgattt gcgatatagt
    28621 tagttacttg ctggcccatc ttagcagaca tcgcttgata taagaccgtc gtagaaagcg
    28681 aaatcccgct gaccatcccc aaatttcggg caaaggaatt catcgagcct gccagtccca
    28741 agttttcgat agtgaccgag ctcataacca tcgtattatt gggggactgg aacaaggcat
    28801 tgccggcacc catgatggca gtagcgacaa tgtatagcca caaaggagag tccactgaca
    28861 aaaacatata catgacctgg gttaccgaca ataagcccaa gcctactaat accaaaatct
    28921 ccgggccaac ccggtcggtc aggtagccgc ttaaaggcgc cccgacgacc atcatcaaag
    28981 gaaagaccat catcaacagc ccagcatagc taggactcaa cccgcgagca ttttgcagat
    29041 aaaaaggaat cactacattg acaaagaagt tggaagaaaa aatcaacaag gctgtaatca
    29101 agctcatggt aaaaatcttg ttcttaaaaa taccgaaaga aatcaatgga gaggctgctt
    29161 tcttttcaac ttttaaaaag ctgacaaagg caatcactga aattacgaac agtacaaggg
    29221 ggactggatg gaaaaagcca ctttcttgcc ccaaaaagat tgctccaaaa aagctgataa
    29281 tcgtagccgc aatggcagca aagccccctc catcgattgt atggccgctc atggtgatat
    29341 cttttggtaa gaatttttca gcaatcaaca tcgttacgat tcccaccggt acattgatcc
    29401 aaaaaatgta cgcccaagaa aattgggaca aaatcaagcc gccaagacct ggtccagcaa
    29461 tcgagcctaa tgaaacaaac gcacctattg ctcccaatgc ccgaccacgt tcatgtatgg
    29521 gaaagacttc cgtgatgatt ccggcatttg tagccatagt catactagca ccaatggctt
    29581 gtaccacccg tgcgaacaat aaaataccta gcgaatgatt gaacccacac aacagcgatc
    29641 ccaagacaaa gatcagcgta ccaatccgaa atactttgat tttcccacgg ctatcgccaa
    29701 ttttaccaaa cagcaacaaa caagaacaga caatcatcag ataaacggac actacccatt
    29761 ccgcttgatt cattggtatg cccatatcat ctgagatggt aggtagtgca atattgacaa
    29821 tactgccatc caatgtggac atgaaggtaa acatagatat agcaatcaaa atccaccagc
    29881 gatttttttg gacttttgca tcttcttgat aactatacat aataggattt cctttctaat
    29941 gatcgtgtag ctaagaacaa tataacctat gtctactgcc caacaacttc tcgcgactct
    30001 agccatagga agcttgagta atttcgattt gatatcctca tgtaaccatg taagttggag
    30061 atgctttatt tgaacttact ctatagtaac tagtgataat tcgctttact ttcttctagc
    30121 aatttctaaa agtcgacttc attgttcttc agattaatag tgtgagctat ctcgtgagac
    30181 gtaagcaaat ttccattcta ctttttccca attttttcat atttatggtg ttgcacctat
    30241 ttgttttcca atttgctttt caaatacatt gctatgatat tcaattccat aggattatac
    30301 taaatatca
//
LOCUS       DAZKOM010000020        30024 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000020 DAZKOM010000000
VERSION     DAZKOM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.020"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..1326)
                     /locus_tag="ACG8NF_000962"
     CDS             complement(16..1326)
                     /locus_tag="ACG8NF_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0471459.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1338..2696)
                     /locus_tag="ACG8NF_000963"
     CDS             complement(1338..2696)
                     /locus_tag="ACG8NF_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0471460.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(2898..4466)
                     /locus_tag="ACG8NF_000964"
     CDS             complement(2898..4466)
                     /locus_tag="ACG8NF_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0471461.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4470..4880)
                     /locus_tag="ACG8NF_000965"
     CDS             complement(4470..4880)
                     /locus_tag="ACG8NF_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0471462.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(4877..5800)
                     /locus_tag="ACG8NF_000966"
     CDS             complement(4877..5800)
                     /locus_tag="ACG8NF_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0471463.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5797..6675)
                     /locus_tag="ACG8NF_000967"
     CDS             complement(5797..6675)
                     /locus_tag="ACG8NF_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0471464.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6662..7999)
                     /locus_tag="ACG8NF_000968"
     CDS             complement(6662..7999)
                     /locus_tag="ACG8NF_000968"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0471465.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8002..9105)
                     /locus_tag="ACG8NF_000969"
     CDS             complement(8002..9105)
                     /locus_tag="ACG8NF_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0471466.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9179..10687)
                     /locus_tag="ACG8NF_000970"
     CDS             complement(9179..10687)
                     /locus_tag="ACG8NF_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0471467.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10653..11345)
                     /locus_tag="ACG8NF_000971"
     CDS             complement(10653..11345)
                     /locus_tag="ACG8NF_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0471468.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11367..12341)
                     /locus_tag="ACG8NF_000972"
     CDS             complement(11367..12341)
                     /locus_tag="ACG8NF_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471469.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12641..13513
                     /locus_tag="ACG8NF_000973"
     CDS             12641..13513
                     /locus_tag="ACG8NF_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471470.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(13971..16169)
                     /locus_tag="ACG8NF_000974"
     CDS             complement(13971..16169)
                     /locus_tag="ACG8NF_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471471.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16401..17393
                     /locus_tag="ACG8NF_000975"
     CDS             16401..17393
                     /locus_tag="ACG8NF_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0471472.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17453..18874
                     /locus_tag="ACG8NF_000976"
     CDS             17453..18874
                     /locus_tag="ACG8NF_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0471473.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            18867..20366
                     /locus_tag="ACG8NF_000977"
     CDS             18867..20366
                     /locus_tag="ACG8NF_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0471474.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20405..21799
                     /gene="uxaC"
                     /locus_tag="ACG8NF_000978"
     CDS             20405..21799
                     /gene="uxaC"
                     /locus_tag="ACG8NF_000978"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0471475.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            21958..22641
                     /locus_tag="ACG8NF_000979"
     CDS             21958..22641
                     /locus_tag="ACG8NF_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0471476.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22643..23479
                     /locus_tag="ACG8NF_000980"
     CDS             22643..23479
                     /locus_tag="ACG8NF_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0471477.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23490..25244
                     /locus_tag="ACG8NF_000981"
     CDS             23490..25244
                     /locus_tag="ACG8NF_000981"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471478.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25520..25861)
                     /locus_tag="ACG8NF_000982"
     CDS             complement(25520..25861)
                     /locus_tag="ACG8NF_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0471479.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25929..28100)
                     /locus_tag="ACG8NF_000983"
     CDS             complement(25929..28100)
                     /locus_tag="ACG8NF_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0471480.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28311..29168
                     /locus_tag="ACG8NF_000984"
     CDS             28311..29168
                     /locus_tag="ACG8NF_000984"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0471481.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29239..>30024)
                     /locus_tag="ACG8NF_000985"
     CDS             complement(29239..>30024)
                     /locus_tag="ACG8NF_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0471482.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         8974 a         6203 c         5328 g         9519 t
ORIGIN      
        1 gaccttatta taatttcaac gattgtcaaa agtattgttt gcagctgtgt tgccagtttc
       61 tatagataaa tcacagcttt tgaaacataa gttttgtact gcttcgattc gcccgaattc
      121 ttttgcagtt atttggcaat gagaaaagca gacatcttct ataggctttt caggaaatgc
      181 tactaaatca aaagctcggg aaggaagtga gtaacctgga gaaaggcatg cctgtacgtt
      241 tttgatgtgt atgtctttga ctttcgtcat ttgctttgat tcaggtattt cttcagccac
      301 tgcttgccaa ctttctggca tttgagcaag gtcattgcta ttttttctat tgtattcatt
      361 atgccagtcc attatccaag aaaaaggaaa ttgtacatca atcatattca gattttctac
      421 cctgatattt tcgatcactc cccctctttc ttttgaagat ttcattcgaa taccacagtc
      481 tgactgaaaa aaatcgatat cgtgaatata tacgtcacta actccagcac taacttcgct
      541 accaatagtt acaccgtatc ctgaacggat tttacatcta gcaacctcga ttcttgatga
      601 ttttcgattg actcttgcac cattgccatc tctgccagac ttaatagcaa tacaatcatc
      661 accacaggcc aaatcacatt catagacacg cacatttgtt gaagaatcaa tatcaatccc
      721 atctgtactt ggtccatcat tgttgcggat gattactttt tctactaata catcgttaga
      781 ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa atcaaatcac gaacaacaac
      841 atgttctgca tgatacagta aacaagcgcg tggacgttta atagcataat ccgcaatcca
      901 acgaagtccc ttttgatcgt attcagctct tgtcccgctt ttttgatctt ttccccaata
      961 cagctcccac caatggtctc cttgaccgtc gattattcct tttccttcta taagaatatc
     1021 tttcgccgag agaacattca aaatcgctgc tggccatttc atttctacgc cggctactcg
     1081 tgtatcgatt tctggaaatg cttcgatatc tgttctcccc ttgaggactg caccttcttc
     1141 aaagataaga tgactcttat cttttaaaaa aagggctccg actaaataaa tgccttttgg
     1201 aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt gctttttgca gtattattgt
     1261 atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca ttgataatcc ctttttttgt
     1321 ccgcaatacg tttctcccta tctttcattg atttttttca attcttcacc aataaataca
     1381 gtttttgaac gattcagtgg gtaacctact agcaaaatga ttgcggatac aaataagcag
     1441 aatgctggta acaagtttgc catcgtataa atcctttctt gtactaaaac tgattggatg
     1501 gctccacctg ttgtagacga ttggtaacca atgagtgcca gcataaatcc gcctaccgct
     1561 cctgcacatg cttgtcctac ttttctcgca aaagaattta ctccatatac tgttccatct
     1621 tctcttaagc ctgtcttata ttgatgataa tcaatcacgt cagtaatgaa tgcccagacc
     1681 atcaaattga ataaaccaga tcctaaagta gcgataaata aaagaatcag atatacccat
     1741 gcatttgtta tatgaaggaa atacatcagc aagtacatac atgaagaagc aagtaacgca
     1801 aatacgcttg cttccttttt accaaataat tttgtcagct ttgtagcaaa tggtgctaaa
     1861 actactactg ttccgtaagt aaataaaaga gcaacagaca ttgcttgttt attatggaag
     1921 taatcattga ataaataagt catattcgtc ccagctaaaa tctgattgat cacaatgaaa
     1981 atgtcaacaa tgactaaaat aatcagtgct cgattagcta acaaaccttt taccaactga
     2041 gaagcagata ctttttcgtt tttgacaact cttactcttt cagtagtcaa tttacaagtt
     2101 aacatatatg cgacaaatgc tattgcagca catccgattg aaatccaaaa gaaatgattc
     2161 cctgaaacga cttgttgtcc actcttgtta tgggtataca ttaaaatcgg aatgaagaat
     2221 cctgtagttg atccgccaat cgcagaacca atgcttcgga acgtggacaa cgaggtgcgg
     2281 tcattaggat ttgagctaat tgctgctgcc attgctccat acgggatatt gattgttgag
     2341 agaaaaatcc cataaacaat gtatgaaaca aaaatataag caatcttcac cgtcattgga
     2401 agatgttgga cgaatggcaa aaacaaaatg acagtcacta agcaaaatgg atacttcatt
     2461 tttctgatcc aaggaataaa tcggccagtt tctgttaact ctgagacatc acataatctt
     2521 ccaaccatca catcagcaaa cgcatccaaa attctcgaaa tcaagaataa cgtacctacg
     2581 attgcccctg aaacgccaag aacattcgta taataaatca tcaagaaact attgactaaa
     2641 cctaaaataa aacagttacc tagatcaccg aacatgtagc ctatcttatc ggaaatccca
     2701 aattcctttt tttcttgttt ttatattcac aatgttggct ttttattgca ttatcttgac
     2761 agataaaaaa caaaaaataa tctaaaaaca ttgatgcata agattgataa ctttttttac
     2821 ataacaatat atacaaacaa aaaaattcac taactagtga aaataagtta gtgaacctca
     2881 tttattaaag aattaaatta cagatgatct tttccatcat acttgaaaaa ctcaaaatca
     2941 gcataacttc ctcttttttt atttagatca tgcactgcta tcccgataaa atttccagta
     3001 aaccctcctg agagtataca catgtttttt tgcggaccta ctttcttcca ttcttcagtt
     3061 gatacgtctt tcaaatgaaa ctgtgctgtc tctttcttta cagtgacttt caaaaaagtc
     3121 ttcttttctg tgagtggaat cgtccaatct tctatttgtg ctactccgtt ttctgattct
     3181 atcatacgta ggcattttcc cttgtcctca ttccaagtaa cataacaata aatataattg
     3241 gattcattta agtacagtac cagtccagcc atctgattaa aattattagg catgtattca
     3301 aatgctgtct ccgcatcaaa agtaaaatcc gtttgccgaa tagctaacaa atgatgttcg
     3361 aaggtagatt gaagagattc accggaaatc agacgtaaaa aacctggtct acattttgta
     3421 tcacaccaat cagcatttgg caaaatgcgt ctggcattcc agttcttctt tagctctcct
     3481 tcaaatgtat ccataaactg atgatctttt tcttgttgta cctcttcatt tgtctcaatc
     3541 attatttctt ccatcggagt tgtctggcta tctttaagtc ttaaccaatc gtcggacgtc
     3601 caatacactt cttgaatagc ggtttctctt cccaaaatag ccgcaccgtt taatggtctg
     3661 gtacaaagat atgtcatgta ccatcttcct gacggggttt gtactaagct cccatgtcct
     3721 gtacattgca atgtagaagc aggtttgtca ctagctgtca gcataggaaa accaggatca
     3781 agctcgtaag gaccagtaat attctttgct cggcaaactg taactgaatg tcctcttcct
     3841 gtaccgcctt ctgccgtcat caaataataa tacccgttac gaaaatacaa atgtggtgcc
     3901 tccgttttcg ctaattctgt acctgcaaat attttataga tgggaccatc aagcttcttc
     3961 ttctctgcat catattcttg taatacgata ccgctggatt tattcggtgt gtccagacga
     4021 taatcccata gttcattgac aagccatttt tttcccgttt caagatcatg gaacaaagaa
     4081 ggatcaaagc cgttactatt taaataaatt ggttcggacc aaggacctgt gatctcagat
     4141 gcagtgatca aataattgtg gacatcttta aacggacggc ctgcactttt gacatctgtg
     4201 taaacaagat aaaacaatcc atcagcataa ctgatctgtg gcgcccatat acttccattt
     4261 gtaggatttc cttgcaaaga tacttgagtg gtcaaaatat ccgttgtatg tgaccagttg
     4321 actaaatttt tcgactcata tactcggatt cctggaagcc attcaaaaga ggagacaata
     4381 atgtaatacc gctctttcac tcttaatata ttaggatctg gattgaatcc tcttaaaata
     4441 ggatttttca ctttgatagt ctgcatgatt caggctcctt ttctcattct tactcgatat
     4501 aaactgaatg ccccagctgc gaaataaaaa aatgcaaaag gaaacgcaat aaactgagtg
     4561 ccaatcaaca atatgaatcc tgttataaaa tacaaaccag tggcagttgt taagtatttt
     4621 cctttagcag tcaaaaacaa tgctactgct aaaaaagcaa gtgaaataaa caacacaacg
     4681 gcaaacagac cagtcatgtc ctgtaataat ccaaatgtat tttctaaaga caaagttgaa
     4741 tgatctacag caagcgactg ataacttttt ttaaactctg acgcagacat attacttaaa
     4801 gtaactgcta acccgccaaa gaaaatcaaa gatagactgc caccgaccat tcctagcaat
     4861 agttcccatt ttcttttcat ttctgaaccc cctgcttttc taaatgtatc cattcaggtc
     4921 ttttgatcga tgtttcataa ttgttattac gttcagagaa tttgacagtc tgttcatttt
     4981 tcttgtcatc ccaattatcc caaccagttg ggtgaacatg cccacctatc ttacacgctt
     5041 caaataagac atttgcatac ggacgccacg gtcttcctaa atagacgttt ctagtatcag
     5101 gttctgcagt caaggtacag ctttcaaaac gataccctgt tttttgattt ttcggcgtag
     5161 atgctgctgt gatgtatcca ggatttttac tggctaagct atggatatga cactgatgaa
     5221 aaacggcatc tgccccacca aatatgaaat ccaccgttcc ttcaatccag caatattcat
     5281 aggtctgacg acaatactca ggattggcag aagccggtgt acaaaaagga gtaccatctt
     5341 tttgcaaatc aggcaacgga cccgtgcata aggtatcctg ataaccagat agtcgacagt
     5401 tttttaaaga tacgcgatct cctgaattga ataaagcaac agcctgtcct accttttctc
     5461 ctggacctgc tgtattttcg atgtgaagat tttctaatac gacattcgat ccttctaaga
     5521 aaaaagtagc acttcggaaa gtccctaact ggctaccgtc aagatactcc tgttttgcaa
     5581 aacgattacc ggagataact actgttcctg ctcccacaag tttaaaattg gataatctgc
     5641 tttctatata ttcatcatat cttccttctt ttactatcaa gacctttgga tggctatccg
     5701 gcaggcctgc caaataatcg attccttctt ggatagtttc aaaatccgtc ggggtatcta
     5761 tagcattctt tccaattatc agtttgtctt tcatcctcat aaatgctccc ttatgaattg
     5821 tttccaaata tttctcattt cttgtgtttc catatgaccg cctgttaaag aaaaagaaga
     5881 aaagttttct ggactaccca ttgattgata ctttttgtcc agcccttttc tcagcttcag
     5941 tacgccttcg tccaagcaca tcgtatcgtt ttttccaact aagctaagac gagggcgcgg
     6001 agcgattttt tcttggattg ctaacgttgt ataagctgtc aatagatttg gcacatagta
     6061 ataaaaccca tgatgatcta ggcggcgatg tagcaaaagt atttcgatgt ccacctgtgc
     6121 cgctagatca atacaaactt tgacacgaga atccaatgct gtcacccacc agcttaataa
     6181 gcctcccata gaaagaccaa tcgttgcgag ccgttgagta tctgtgtctt cccttgtctc
     6241 caaataatcg atcaagctga tcacatcata aatacgcatt ccccataacg tttttcctgt
     6301 taataaaaat tgtttgaaca actcgctttc agaaatacct ccgcgttctt caaatcccca
     6361 agcatcaatc gaccaaacag catatcccat tcctgtcagt acttctgcaa aagatggaga
     6421 agctaagtaa ggagcaccat ggatcaattc acttttcccg actgaaaaat ctccgccgtg
     6481 agaatgcagg taaaggaccg gcggaaacgg tccttctttt tctggataag catagagggc
     6541 tggtactgtt tccaattcat ttaagtacaa ttgatagtat tcaagcataa aaccgttttg
     6601 tttttctcgg tataatcgtt ttccactagg ggtatgtctg ctagatggag cacctaaaaa
     6661 atcattcaac tgcataagca aagtttttac gaacaaccag ttcatccata ttttctgatt
     6721 gatttgattg gaatttctca atactgattt ctttagtatt ttctaataga aatgctggtc
     6781 cctcgtgatt ttcaatcgac atctggttaa aggaaacctt acccgcaaag ccaatataaa
     6841 atccttgtct gctcatttcc tctattcctg tcatcatagc aggaacaccg ggaactgcat
     6901 cttgggccat cgagacatcg atattgtcaa atgtgatttc ttgtatgaat tgttccgcta
     6961 atccataaat aaaacctgca gaagcatgga cattccttgc cgtgatattc gaaaaatgga
     7021 tccttctgaa tgctggtgtc cgttcatcta tcggataagc ctttttttcc catacatacg
     7081 gttctttccc tcgcggacca cagaaataat aaagattcag tataaatgga cacatcacat
     7141 tatccatgac tatattggat actcgaatat cttcaacgat cccgcctcga cctcgtctag
     7201 atttcaatcg tattcctcga tcagtttctt ggaagataca atttgaaatg gtaatattac
     7261 gaatactacc actcatttca cttcctaata cgacaccgcc atgtccatgg accatcgtgc
     7321 aatttgtgat cgtgatattt tcgcaagcga tcctttcgta cgtatcttca gtccctgcct
     7381 tgattgcaat acagtcatct cccacgtcaa tatggcaatt actaatacgc acatttttgc
     7441 atgattctgg atcaattccg tctgtatttg gtgaatccgc tgggttcaag atcgtcaagt
     7501 tatcaaacgt tgcattactg caaagaatcg ggttgatcgt ccaacttggt gactggatca
     7561 atttaatatc tttcacagta attcgatgac agttatggaa actcatcaat ttaggacgag
     7621 gataagcgag attatctggt tcatttctaa aagtatgcca ccattttttt ccatttccat
     7681 ctaatgtacc gaaaccagtg acagagatgt tctctacatt ttgagcatag atacaggaag
     7741 cataaacttt tcgatggact ccttcccaac gtgaatgaac aacaggataa tcttttggat
     7801 cgtcagagaa tttcagaacc gctcctgcgc ttaaatgaag ttcgacattg ctttttaaaa
     7861 ataaagctcc tgttagaaat tcacctgctg gaacaactac ggtcccgcct ccgtcacttg
     7921 ctgcagcatc aatggcttgt tgtattgctt cagtattcaa ttcatcaatt gatgcgccaa
     7981 atgtcaaaat atcatacata tttataatgc ctccacttca atcaatgctt gcaagaatgc
     8041 accaacgcct ttttgatcat tacaaataat tggttcactg atgtagtaag catatgaacc
     8101 atctcgctga tctgctccgc ctaaccctgc tacttgacag tttttattca agttcagcca
     8161 gccttctttt gtttccagaa caaattcatc aagtaatcct tgatgtgatt tcttcacttg
     8221 tggaagccat tcttctttgt tcagcagtcc caaacgtatt cctttagcca ttgcgtaaac
     8281 aaacatacta ctgccggaag cttccagata atttcctttc cgttttgttt ctgttgttac
     8341 ttgataccat gtcccgctgt tttcttcttg atatggaagt aatgcagcca gtgtcttttc
     8401 tagaatttct ttcagcaaag aacaatctgt ttcttcatga agtaattcat aggtatctgc
     8461 tgcagccatc aaataccagc caatcgatcg gctccaaaaa tgatgggata gacctgtttt
     8521 tggatcagcc catggttgga ccttcttttc atcccatgca tgaatcagca aatttgtttt
     8581 tggatcgaat agatgtttat aactgatttc aaactgtctc aaaacatctt ggtaatcttt
     8641 ttcttcacta aaattctgga taaattcagc ataaaaagga gaacccatat ataagccatc
     8701 caaccaaatt tgataaggat aaatttcttt atgccaaaaa gctccttcgg atgtgcgtgg
     8761 atggtttgcc aactgctggg aaaggagttc tgctgctttt ttatattttt cttcgcctgt
     8821 ttctttgtac aataaaaaga aaagctttcc tgtatttaca tggtcgatat tgtattcttc
     8881 aacgctgtag ccacggatcg ttccatcttc ttgcacgaaa tagtcgatat tgtcttttat
     8941 gaaatcaaaa taaatttttt cttgggttcg ttcccaaagt aattggaacc ctttaagtac
     9001 gacgccatag tcataagacc attttccttt ataccacttt tcttcatata agcgaggtgt
     9061 tctttccatg atactttttg ctaatttctt tgaccagttt tccattattt gctctccttt
     9121 aaaaattaaa ataggatttg ggacaaatac caagaagtat ttgtcccaat cgtctatatt
     9181 actgtgccgc tgctttgtat gcttcctcgt actcttgtgt gattcgatcg cctccttgtg
     9241 ctttccagtc ttctacagct tttttgaatc catcttcgtc aagctgtccc atgatgtatt
     9301 gcaccgtagc atcaatgacg attttttcaa tttctgatcc tacttcattg ttcgtcggtg
     9361 aatccaatgg cacagaagga tccattacag caaattcttc attttctttg atcaattgat
     9421 tagagaattc tttttcaggg tctgtatctt tgaaagagaa cgtcacttca ctaggacgag
     9481 agcttgataa cggttgcacc tcttgttgcc aaagatccat atcgataaat tcgatagaac
     9541 catcatcatt caattcataa tgcgttcctt cgataccgcc agtcatcaat ttatacacat
     9601 cttcttctaa aagatcatca acaaattgaa gtaatttctt caattgtgct tcgtctttta
     9661 cttgagattt gggaaatgct agcaagccgc caataccatt tccttctgac caaacatgat
     9721 aaccgccgcc ttcaccatct gagatcttgt ttacaggcac taattccata tcatcttgaa
     9781 taccttgacc catcgtcttc agattcttga tatccaccat tccagtgtag attccagttc
     9841 ttccttgtgc aaattgttct tgctgatcag tcttttgggt aactgcaaag tcttgtgcca
     9901 aatagccatt ctcaaataga tctcttgaaa aattcaaagc tttcatatac tcttctgtat
     9961 caaattctgg agtgaatttg ccattatcgt ctacagccca gccatttggt gctccaaaat
    10021 aagaagtgaa aagtttaaag ctggtataac gaatatcact aggcgcacga tcgccaaaac
    10081 caactgtgtc gtccacacca tttccatcag gatcgttttc tgtaaattgt ttagctactt
    10141 cataaagctc atccatggta gttggaacat ctagtcctag attatccaac cagtcttttc
    10201 gaatgactaa gccagcacga gcgtagtctt tttgtatcgg tacgccgtac aaggcaccgt
    10261 cgatactcgc agcttcgatt ctatcttcag aaatttgact taaattttta tagtctttta
    10321 aatattctga tacatcccag aatacgcctg attttaacgc attgcggaca gaagagtttt
    10381 tcaacatcgc aatttgaagt gtaacgatat ctcctagttc atcagaagcc aatgcagttg
    10441 ttaatctttc ttcttttgaa gcatcaggaa tccagttgaa tgtcagttcc gtatcggttt
    10501 tttcttcgat caagttcaca atcgtgtctg taggcggtga agcatgatgc aaaatattta
    10561 accaagtgat tttagctttt ccatcttctg tctctgaaga actgttactt gaactgctgc
    10621 cgcatgcggc aagtgctaga attcctgcaa aactaagacc taattttacg agcgttgctt
    10681 ttttcataaa ttttctcctt cactgtttta ataatatttt tgttttcttc tgttttttct
    10741 gtatatgagt tcttccattc caaatggcaa aatatagaat ttgctgtcca cgtgactgcg
    10801 gaaaagaaga gactgccacc gataaaggtt aaaacacctg gcattgtttg gatgatcatc
    10861 agataaatcc ctaccatact taatatcaac actaatgagt aatgaagatg tgagaatccg
    10921 aataaaaatg ccattttcat tttgaaaaaa tatcctttcc aatcataatg agccatgatt
    10981 gggaaaatat agagtgctga gagcacatat atgaacgcag caatatacaa agcgatccta
    11041 agataccaat tgtgtatgaa agaaagatcc acaagtaaaa caatcccgat ggcgccatat
    11101 atccagctca tcagcaaggt ttcacgatag ttttctttaa aatatgtgaa gtacgttttc
    11161 gtcaaaggaa acgtttcttt tgagcgcagc cattttctaa tcactgcaac catcgcatac
    11221 gatgcaggac cgaaagtaaa aactcccaaa cctgcgatga ctaaaccgcc ccacagaaaa
    11281 ttcaaataaa ccaactgtaa aatacgattt agccttccgt ttattttatc taatgactgc
    11341 agcatatctt tccctctctt tctatcttag taaacgccgt cttctcctag ctttttggca
    11401 atcttattag ctaaaattac cataaacaat cctacgaccc ctttaaacaa tcccattgct
    11461 gtactgaaac ttaactgtcc attcttcaat ccagctgtat aaatataggt gtcgaaaatt
    11521 tctgcaacgc ttcgattcaa tgaattcaat aacaagtaca tatgctcgaa ccctaagtct
    11581 aatgtctgac cgatttttag aatcagcaag gtaatgatta ctggacgaat tgccggcaat
    11641 gtaacatgcc atgttttgcg taagcgtcct gctccatcca tttctgcagc ttcatacaac
    11701 tgtgtatcga ctgcagttat cgctgaaagg tagatgatcg tagaccagcc caactctttc
    11761 cagatcactt gtccgatata tactgtgcgt gtccattcag gggatgttag aaaactgatt
    11821 ttttcaaatc ccattgttgc aaggaattca ttcaataccc ctccgtcaac attcaaaaat
    11881 acataagtaa tcgaaacaat gattacccaa gacatgaagt gagggatgta aatgatcgtt
    11941 tgaatcccgt ttttcaatcg tttgttcgtt acttcattta atagtaaaga caaaatgatt
    12001 ggcatcggaa aaaacacgaa gatatttaat ccgaacaaga tcaacgtatt tcttaataat
    12061 gtaaagaatg tcggttctgt aaacaaacga atgaaatgtt tgaaaccgac ccaaggactt
    12121 cccattattc ctaaaaatgg ttgataatct tgaaaagcaa taacgagtcc ccccatagga
    12181 agatacctaa aaatcaagaa ataaaggact cccggcagaa tcatcacata taaaaattta
    12241 ttcgttttga ttcctgcaat tatttttttt cgtcttctga gcttttcttc ttttttcgat
    12301 agcgtcagtt gaaatggttc ttgtttcttt acactttcca taagcccctc cttagttttg
    12361 tgaatgtttg ctgcttatga cttcatgata gataactatc tgaattacgt atataatcat
    12421 ttttaccgta agtaaaaacg cttacatagc aatgattttt gtgctataca aaacgataaa
    12481 gtgtattttt tcacaaataa actacgcata gcaaaccttc tatttttttc tatctgacac
    12541 ttagcatagg aaatgattat atacgtaatc tttcaccgat gataaattga acatatagaa
    12601 acagaaacat ttaatttatt ttgaggagga gcctatctat atgaaaaaga aagtctctat
    12661 aggagaaaga agttttacac ttttcaacaa tacgtttcta attttgctag ctttgatctg
    12721 tatcgttcct tttttaaata tcatcgctac atcttttgct tctacacaag aagtcgtcgc
    12781 gaaaaagttt atcctttttc ctactacctt ttctttagac gcgtatcgct acattctttc
    12841 tacgccgacc atttttagag cacttgctgt ttcgattggt gttacaggtg tcggaacgat
    12901 cgtcagtatg tgtgcaactt cactgatggc ctacggtctt tccagaaagt atttgtttgg
    12961 tcgaggtttc gtcaactttc ttgttgtatt ttctatgttg ttcagcgggg ggatgattcc
    13021 aacgttctta gttgttcgtt ctctagggct ggtcaattct tactgggcca tgatcctgcc
    13081 tgtggctgtt aatgcaatga atatgatcat tatgagaaac tttttccaag ccttgccgga
    13141 tagtttggaa gaatcagcaa aaatggatgg ttgcacagat tttggtgtat ttttcaaaat
    13201 tatgctgcca ttagcgttac cgtctattgc tactatctcg cttttttatg ctgttactta
    13261 ctggaacact tatatgactg ctattttgta tatcaacgat tcatctaaat ggccgataca
    13321 gattctttta cgccaaatcg tcattgtatc aagtgggatg caagcagaaa gtagtgccgt
    13381 tgatgtgatt ccaccagcac aaacagttaa aatggctgtt atcgttattg caacagtgcc
    13441 tatgctgatt gtttatcctt ttgttcagaa atattttgtc aaaggagcac tcgtaggttc
    13501 tgtcaaaggg tgattatgta ctgaacgcaa aatggtagtt gtgtcagaag tggtcagctt
    13561 caagaaataa gaaggcattt acgaaaattg cttcttaaat ttttgtgaaa tttcggctta
    13621 tttttcgaag aatctactcc tggaacaccg tgtatcagag gttgtgaaag agacgttcag
    13681 ccttgagcga tgataaaaac tctcacaaac attgcgacga gtaacccaca ggagcaataa
    13741 gaagcgattt tgaaaaatag cttcccatat ttcacaaaat tatgccttat tgtcgatagc
    13801 ctgtctcttg aacgccattt attcgtaaaa aagaactgtg acaagacttt tgtcacagtt
    13861 ctttttgcgt gcttagcctt gaaatttaac tatccataaa tacaaatact agggttattc
    13921 taacttcttt tattgcttga atcagtccac aagaaaaaca gcctcttgat tcacaaatat
    13981 ttttttcgat attctcccgg cgtaatcgat tccttctttt taaagaaacg aataaaattt
    14041 tgcggatttc gatattgcaa tctttctgca atctctttta cggagaggtt agtctctttc
    14101 aaccattttt ttgctacctc taaacgatga ttttgaacaa aatcaccaaa tttttctcct
    14161 gtttcctttt taaatacatt acttaaatag ttagggttat aatgcaacct atcagcgatc
    14221 acatctaaag acagatcacg atcatattct gtgtagacga tatgcataat attctccgac
    14281 aaatttttaa attcatgatc agtgattgct tgagagcatt ctgtgattgg cagaactaat
    14341 tgttctaaaa caagtttttc tagttctttg ggatgataat tgttcagtgc ttttacataa
    14401 atttgtttca tgttatcaaa gttttttgaa tctactccca ataactgtcc taattgaacg
    14461 agttcattga tcaagcggac cgaaactact tcccgactca aaggattttt attttgtgca
    14521 aacaatgcat caatcaattc atgtactaaa ttcttcactt cctgatttcc agaacgaatg
    14581 gcttcaaaca atcgattttg ttcttctatc ggatatctaa taagtgtttt ttcatgagca
    14641 gcaggaacga tttcctgata aaaaataatt gaacttggac cagtatttac acgataatac
    14701 agggcttctt ttgcccgatc aaccgattgc ttgctttcat tcagcgtttc gaaccggtta
    14761 ctgatgccta tactgatagt taaattcaag tattctttta cacgtttttg aatgagtcgg
    14821 caatactcca tgactttttt gcttgcttct gcatcatctt tatccattcg ataaatagtg
    14881 gcttgcattt cttcattcaa aacgatcggc agcattcgat tttcctttgg cacaatttcc
    14941 gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct ccccaccgtg actatcttct
    15001 aacagatcga tttgaaccaa agctgtataa tagcagtcat tttgagaact ataaccaaat
    15061 tgctgcaacc gctgatctag ttctcgcctt tctactcgat ttctaaataa actaagtaca
    15121 aatagcgttt ccagctgtgg cttttgggta actaagtgtg cacttaacgc ttctttttct
    15181 cctatgatcc gactgactga ttgagcgacc aaatctagtt ctttcccact gaatcctttt
    15241 gatttaagat tcagacgttc ctgtatttga gaaatcggac gggcaaatct gtccgaaaat
    15301 aagtaggaaa gaagaccaat gagtcctacc agtgttaaag acactgcaac tagcccgatt
    15361 ctcagattgc ggatcattgc gccaattgca ctagagtcaa cttctaatct gtaaatccaa
    15421 ttattataat ctgatttcag tactatatac gttttatctt tttctcttat cactttcagc
    15481 tgattgtaat ctagatccac gtccgttaat acagttggtt gctctttatt ttttactgtc
    15541 tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa gtatgtcttc tccactttca
    15601 ccaacaactt gttctattgt atgtttctct atcgtagcaa ttcccaatgc atatttttct
    15661 ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt tgtttttctt ttgccaaaat
    15721 aaattatttt tgttatttat gtacttctgt tgatactttt caatttcctc ttcggtcagt
    15781 tgactcaacg atccattgat tactccccat ttaccagcca aactgactaa atcataatta
    15841 ttatcatcca taacaatcgt ttctataaaa tttagttctt tcctgatttc agaataagtg
    15901 gagtaatctg tatatgtcag cggatgcttg aatatgtccg agaaattttt ggtcgaacca
    15961 tacgtagaaa acgaatattc aatcgttcta attttccatt caacgttagt tttaacttgc
    16021 ctgagcaaat tctttttgtc tgttaaataa ctcgattcga tcgattcata ggtattctgg
    16081 taaatgaata aactaaatcc tatgacaagt acggctccta ataaaaacat cggtaaaaat
    16141 attttataaa atactgtact tttcttcatt ttcttcctct cctcctttta aaataaaagg
    16201 ctttcatttt tctgtaaaat tgatactcat ctaattaaat gagcattccc cgctttcaag
    16261 aatagcataa ttaaaaataa aaatccaagt aatttttaac gtgtgcaatc ttttcatatc
    16321 aaagatctat tgacttatcg tagttatcgt aatatgatga atgcgttaac aaaaagtgaa
    16381 aaagtaagga aaatgatgga atggtaacga tcaaagatgt cgctaaagca gctggcgttt
    16441 ctcatacgac agtttctcgt gctttgaata acagcagttt aataaaaaat gaaacaaaac
    16501 aaaaaatacg acaactagct aaggaaatga attattcgcc aaactatagt gcaaaaggtt
    16561 tagtgaatca aaaaaaatat ctaatcggac tatttttctc aagtttgcat aaaggaactt
    16621 catcaaattt cctagcagat tccattgcag gcatccattc agtattagac acaaattaca
    16681 gtttatctgt tgaaagtatc gatgttgttg atttaacaga aatcaacctg caacgctacg
    16741 atggactgat cgttatgagt caaagtgatg atgatcagcc tttcatcgac tatctcaaag
    16801 aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa tgatccagcg gttatcaacg
    16861 tcactgccga tgatgcggcc ggtgttatgc aggcagtaga ttacggtatt ggatgcggac
    16921 atacgaagat cgcttatatt ggcggaaaga aaaattttcg ttcgacaaaa gaacgggaaa
    16981 aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt caatccagcc tattttttaa
    17041 caggagatta cagcttagaa agcggcctcc aaaacatgga atacctatta tcactttctg
    17101 acctgcctac tctggttttt tgcggcaacg acgacatagc gattggtgca ttgagagctg
    17161 tacatttagc tggcctccgt gttccccaga acatttctct gatcgggttc gatgattctc
    17221 cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg taagcctttg aaagaaatgt
    17281 gtcaagaagg aacaaagctt ttactagatt tgatcgaagg caggacgatc gaacaatcta
    17341 aaattgagct ctccaccttg ttgatccttc gtgagtcgat caattacttg taaactacaa
    17401 gtaattttat ataaaaatgt taacgtgtgc ataacacaga aaggaagttt atatgaaaaa
    17461 actgacaaaa gaattagtga aaaaaaacga agcacctgta aaagttcttc agtttggtga
    17521 aggcaatttt atgcgtggat ttattgattg gcagattcag cagctgaata accaacaatt
    17581 gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa ggtcttggaa atatgatgaa
    17641 ccagcaagat aatttatata cggtcatttt acaaggactc agcaatggcg aaatcgttga
    17701 tacttccgag attatcaccg tagtagaaca aatgatcaat ccttatgatg aatgggatgc
    17761 ttatctatct ctagcagaaa atgacgactt ggcctatatc gtctccaata caacagaggc
    17821 aggaatccaa ttcgtggaga ctgataattt aacagatact cccccacaga gcctcccggg
    17881 caaactgaca gcctttttat accgacgttt caaattaaac aaagctggct ttaccatcat
    17941 tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa gaaatcattt tacgctacgc
    18001 agaaaaatgg gatttagaac ctgcttttgt tgagtggtta gaaaaagaaa atgttttctg
    18061 ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc gaagatgcag gtgttttcaa
    18121 tcaacggcat aactatgaag atcaattgat ggtcaaagca gaaccattca tgctttgggt
    18181 gatcgaaggg cctaaagaat tggagaatca actaccattc aaacaggctg gcttgaatgt
    18241 catcatcaca gatgatatga ctccttatcg tgaacgtaag gtgcatttgc ttaatggacc
    18301 gcatacagcg atggtccctc ttgctttgtt ggcggaattg gaaactgtag aagaagtaat
    18361 gaaagatcct ttattctccg catatgttga tcagttattc aatctagaat tgattcctat
    18421 gctttcttta cctaaagatg aattagccat ttatgctgac caaatcaaag aacgtttctt
    18481 gaatcctttt gcccatcaca aattagaagc gatttcatta aatagtgttt caaagttcag
    18541 cacccgtctg cttccagtgt tcaagaagta tatcgaagaa caaaatcagg taccaccgtt
    18601 aatcactgtt tcactagctg cgctacttct tatgtatcga ggcgatcaag taaagccaca
    18661 tgatgatgaa aagacgataa gcgaatttac tgatgcttgg tctgataatg gaacagctat
    18721 tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat ttgtctcaaa taccgaatgt
    18781 gacagataca gttcaagaaa tagctgcaaa gatcgatcaa aaaggtgtcc ggtcagtatt
    18841 acaagaaatg aaaggaacga tggtccatgc ttgaaaccct gcaaccaaca atgaaattaa
    18901 atcgacgaga cagtgtggtc gtcgcattaa cccctattcc aaaacaaaca caactgacga
    18961 ttgatcagca aacgatcacc actcttgaag atatcccgca aggtcataag attgcattgg
    19021 gtgatttgaa agctggagac aacgtgatca aatatggcta tccaatcggt catgtgacga
    19081 cagacgtaac tgccggtcaa tggctgcaca ctcataatgt aaaaaccaat ctttctggtg
    19141 aattggatta tcgatatgaa aaagatgtcc atccaagtca ttaccttttt gagaaccgaa
    19201 catttcaagg ttacttgcga aaaaatggaa aagtagggat tcgtaatgat ttgtttttag
    19261 tacctaccgt tgggtgtgtg aatggcattg ctgaactgat cgtcaaacag ttcaaggaaa
    19321 aacatccaga tctgggacag tttgaccata ttacgatttt aaagcatcct tatggctgtt
    19381 cgcagcttgg aaaagatcat caaaatactc gagaaatttt agcggacgca gtaaatcatc
    19441 ccaatgccgg aggagtttta gtatttggat taggttgtga gaacaataca gttcctgaat
    19501 tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa atttctagtg gctcaagatg
    19561 tttacgatga gatcgaacaa ggtgtggctc tattagaaga gcttttaaca gcggcggaaa
    19621 atgaccagcg catatcagtc ccgctctcta agttgaatgt cggtttgaaa tgcggcggtt
    19681 ctgatggtct atcgggaata actgctaatc ctttacttgg tgcattttct gactacttga
    19741 ttgctcaagg aggcagcacg atcttaacag aagtgccaga aatgtttggc gcagaacaag
    19801 tattgatggc acgagcggaa aatgaagaag tttttgactc aatcgttgac ttgatcaatg
    19861 acttcaaaca gtatttcttg tcttacgggg aaccagttta cgaaaaccca tctcctggta
    19921 ataaagcagg tgggatcact acattagaag ataagtcttt aggctgcacg caaaaatcag
    19981 gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa aatcagaaaa ccagggctta
    20041 gtttattgca agctcccggg aatgatcttg tagctgcctc tgctcttgct tcatccgatt
    20101 gccagttggt cttgtttacc acaggaagag ggactccgtt tggctcatat gtcccaacgg
    20161 tcaaggtatc tacgaataca actctttttg aacgcaaagg tcattggatg gattttaatg
    20221 caggcgtctt attagaacaa ccaatggaag taatcttaga atcgtttgtt caaaaaatca
    20281 tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa tgacgttcga gaaattgcca
    20341 tttttaaaaa cggagtaaca ctttgatttt cataaaacag tataaaaaga aagaaggaag
    20401 atttatgttt ttatctgatg attttttatt aaaagacgat tgggcgaaaa agctttacca
    20461 ttcctacgca aaaaacatgc ccattattga ttatcactgt cacttagatc caaaagaaat
    20521 ttacgaaaac aacaatttca gcaatctgac agaagcttgg ttaggaggcg atcattacaa
    20581 atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa atcacaggaa atgcttctga
    20641 ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa attatcggaa atccgcttta
    20701 cagttggtca catcttgaat tgaagcgttt cttcggtatt gacttgttga taaatgaaga
    20761 aaatgcagag aaaatttgga cacaagcaaa tgaaattttg gcagctcctg attttcgccg
    20821 tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt acaacagatg atcccgcaga
    20881 cgacttgcac tatcatcagt tgcttgctca tgaagaacca gatttgaagg ttctaccttc
    20941 attccgtcca gataaagcac tcaatattga aaaagaagga tttaatgcat gggtcacact
    21001 actggaagaa gcagctgacc aaacgatcac cacctatcaa gaactgatag aagtcttagg
    21061 gcagcggata gaatacttcc atcaacatgg atgtcgtgtt tccgaccacg ctttagacat
    21121 cttacgctat aaagaagcgg atgaaacagt attagaagag atttttcaaa aagcacgagc
    21181 taacaaacta ttatcttcag atgaaatcga tgcttatcgt acagaaacac tgatacattt
    21241 gacccacttc tatcattctc ataattggac gatgcagctg cacatccatg cttatcgtaa
    21301 ctgtaataca caaatgttgg aaaaattagg gcccgatacc ggctatgatg gaatgaatga
    21361 tctttctttg actattcctc tgcaaaaatt attgaaccgt gcagaagaaa cggatcagtt
    21421 gccaaagacg atcctttatt cattgaatcc gaatgactat cctgcgattt tagctttgat
    21481 gggctgtttc caaaaagaac agaatggaaa actgcagtta ggttctggat ggtggtacaa
    21541 cgatactcga gctggtatgc gcgatcaatt gacacaattt gctgatggta gtgctttggg
    21601 gaattttgtc ggcatgctga cagattcaag aagtttcctg tcctacacta gacatgaata
    21661 tttccgtcgt gttctttgtg aatttttagg cgaaatggtc atgcgaggag aagcacctga
    21721 agatgaacaa cttttaggaa gtctagttca agcgattagt tatacaaatg ctaaaaatta
    21781 ctttggcttt tttgcctaat tgattaatag taacagtaac gaatcaaaaa caggatagtg
    21841 aaataacgga atccgtttat ccacgtttta gagaagaaga cggaccaaat ttgctccgtc
    21901 ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag agataacatg
    21961 cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa caagcgtccc
    22021 gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca gatcaataac
    22081 caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt agcccgtcta
    22141 gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa cgaccagaaa
    22201 atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct gcagatattt
    22261 tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt gacgcgacga
    22321 gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc aaaagcgatg
    22381 cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag atcacaagaa
    22441 ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct tatacctgat
    22501 acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc agaaggcgct
    22561 gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc tcttgctaat
    22621 tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg cccacgattt
    22681 agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact tgtcccatat
    22741 ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca tgacacttgg
    22801 gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt ccattttagg
    22861 atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat tggcaacttt
    22921 caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta cggaaaccgg
    22981 tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc agatcgccag
    23041 aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg ttgctgttga
    23101 agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa tcgatgaagt
    23161 agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata aagaaaatca
    23221 tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt atattacagc
    23281 gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac ctgttggcaa
    23341 gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc ctctaatgta
    23401 cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac acttgcagtt
    23461 tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa agttatcaaa
    23521 aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat tattggcgtt
    23581 ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta atgccgatcg
    23641 ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg caggccattt
    23701 atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg aattattttc
    23761 atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga gcgaccctta
    23821 tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct ggacgaatcc
    23881 agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa gtatttgtca
    23941 gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt tataaaagtc
    24001 ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt actctctttt
    24061 tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa gagacttctg
    24121 caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg ttagaaaaac
    24181 acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga ttccacttag
    24241 atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata tttcagctga
    24301 tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc ggcgatgtcg
    24361 tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg atcacgctga
    24421 ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg ttgattgctg
    24481 gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga aattttcgtt
    24541 ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat catttttctc
    24601 tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct ggaaaatcta
    24661 cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt tttctagatg
    24721 atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc gttgttcctc
    24781 aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc ttgaaaaacg
    24841 tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca ctgattgacc
    24901 agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg tctggtggcc
    24961 aaaagcaacg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc attttgctag
    25021 atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa gccctgaatt
    25081 acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg atcaaagagg
    25141 cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact tacgaaaaac
    25201 tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa ataggagcta
    25261 tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct gccaactcct
    25321 cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat tgttccttgt
    25381 aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa gagacagcct
    25441 atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt cacaccctct
    25501 gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc atgatgatac
    25561 ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag tcattgatta
    25621 cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct tcgtcgctga
    25681 actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg aagtctttga
    25741 acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc aacgctttga
    25801 attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag atgtttgaca
    25861 tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt tattcgtttt
    25921 caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc accaattccc
    25981 tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc ttgttcacca
    26041 tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc tgtgtttcca
    26101 acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac actaaatcct
    26161 gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt cccgacaaca
    26221 tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc catccatgtc
    26281 gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt tgtggcatcg
    26341 aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg attcgcagca
    26401 gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc ggctgtttcc
    26461 tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc tccagtcgaa
    26521 tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc aaatgcactg
    26581 taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag ggctaagcca
    26641 taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc atatcctttt
    26701 tgtaaaccga tctcatgcaa aagccataca gtaggcaaat tcaaactttg tgcaacagct
    26761 tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc gtaataactt
    26821 tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc tggtgtatat
    26881 acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc aaagctgaat
    26941 cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc tgtattcgga
    27001 tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc tgggaataat
    27061 gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa agctgtatag
    27121 attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt aactgcttca
    27181 tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt atcgtatgtg
    27241 tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc ttttgtgatt
    27301 tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc attttttggg
    27361 tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt agcagcctca
    27421 gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc ttggacaccc
    27481 cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc ttctttgctg
    27541 tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc aaacgtttgg
    27601 tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc tccgccccct
    27661 gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt gatatcaaac
    27721 ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg gatataggga
    27781 gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc agcttcttcg
    27841 tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt ttcgacattt
    27901 gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat caaaccaatc
    27961 agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca tcgatggaat
    28021 tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt tatcttttct
    28081 aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat cgtcttattt
    28141 taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg tttttttacg
    28201 ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt cttaaatatg
    28261 ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag gagtacactt gtgaaaatca
    28321 cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt gaaaaggaat
    28381 ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt tggatcaatc
    28441 aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca ttgttattcg
    28501 aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac atccgtgttt
    28561 tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag actcatccaa
    28621 atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc gacactaaac
    28681 agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc gttttatttg
    28741 caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa atgatctatc
    28801 ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc accaataaaa
    28861 ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat ggtcagagcg
    28921 ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc tattgtgttt
    28981 tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga catccgatcg
    29041 ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat gcattagaac
    29101 tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta cctggattat
    29161 gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga ctctgttctt
    29221 tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca aactaccgat
    29281 aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt gaggtgtttc
    29341 catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga gatgatcctg
    29401 attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg tatagttgat
    29461 cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt gcggcagatc
    29521 ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc cttctaaaca
    29581 atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt tcacccaaat
    29641 atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca cgacacctgc
    29701 accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg ctgctccttg
    29761 cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag gaatattgta
    29821 gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg gagttgccaa
    29881 aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg agccaatccc
    29941 gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg tttcttctgg
    30001 tgttgtggta gggatactta attt
//
LOCUS       DAZKOM010000021        29057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000021 DAZKOM010000000
VERSION     DAZKOM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.021"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            315..635
                     /locus_tag="ACG8NF_000986"
     CDS             315..635
                     /locus_tag="ACG8NF_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0471483.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            789..1694
                     /locus_tag="ACG8NF_000987"
     CDS             789..1694
                     /locus_tag="ACG8NF_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471484.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1691..2464
                     /locus_tag="ACG8NF_000988"
     CDS             1691..2464
                     /locus_tag="ACG8NF_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0471485.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2645..4069)
                     /locus_tag="ACG8NF_000989"
     CDS             complement(2645..4069)
                     /locus_tag="ACG8NF_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0471486.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4280..4726
                     /gene="nrdI"
                     /locus_tag="ACG8NF_000990"
     CDS             4280..4726
                     /gene="nrdI"
                     /locus_tag="ACG8NF_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0471487.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4791..6773)
                     /locus_tag="ACG8NF_000991"
     CDS             complement(4791..6773)
                     /locus_tag="ACG8NF_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0471488.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6787..7683)
                     /gene="murQ"
                     /locus_tag="ACG8NF_000992"
     CDS             complement(6787..7683)
                     /gene="murQ"
                     /locus_tag="ACG8NF_000992"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0471489.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7686..8747)
                     /locus_tag="ACG8NF_000993"
     CDS             complement(7686..8747)
                     /locus_tag="ACG8NF_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0471490.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8958..9485)
                     /locus_tag="ACG8NF_000994"
     CDS             complement(8958..9485)
                     /locus_tag="ACG8NF_000994"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0471491.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9552..11054)
                     /gene="eat(A)"
                     /locus_tag="ACG8NF_000995"
     CDS             complement(9552..11054)
                     /gene="eat(A)"
                     /locus_tag="ACG8NF_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0471492.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11312..12433)
                     /locus_tag="ACG8NF_000996"
     CDS             complement(11312..12433)
                     /locus_tag="ACG8NF_000996"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0471493.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12559..13500
                     /locus_tag="ACG8NF_000997"
     CDS             12559..13500
                     /locus_tag="ACG8NF_000997"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0471494.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13650..14546)
                     /locus_tag="ACG8NF_000998"
     CDS             complement(13650..14546)
                     /locus_tag="ACG8NF_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0471495.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14815..16155
                     /locus_tag="ACG8NF_000999"
     CDS             14815..16155
                     /locus_tag="ACG8NF_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0471496.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16373..16816
                     /locus_tag="ACG8NF_001000"
     CDS             16373..16816
                     /locus_tag="ACG8NF_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0471497.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17320..18738)
                     /gene="nhaC"
                     /locus_tag="ACG8NF_001001"
     CDS             complement(17320..18738)
                     /gene="nhaC"
                     /locus_tag="ACG8NF_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0471498.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19141..20364)
                     /locus_tag="ACG8NF_001002"
     CDS             complement(19141..20364)
                     /locus_tag="ACG8NF_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0471499.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20395..21234)
                     /locus_tag="ACG8NF_001003"
     CDS             complement(20395..21234)
                     /locus_tag="ACG8NF_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0471500.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21251..21931)
                     /locus_tag="ACG8NF_001004"
     CDS             complement(21251..21931)
                     /locus_tag="ACG8NF_001004"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0471501.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21933..23006)
                     /locus_tag="ACG8NF_001005"
     CDS             complement(21933..23006)
                     /locus_tag="ACG8NF_001005"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471502.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23062..23300)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23343..23576)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23653..24324)
                     /locus_tag="ACG8NF_001006"
     CDS             complement(23653..24324)
                     /locus_tag="ACG8NF_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0471503.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24411..27059)
                     /gene="mgtA"
                     /locus_tag="ACG8NF_001007"
     CDS             complement(24411..27059)
                     /gene="mgtA"
                     /locus_tag="ACG8NF_001007"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0471504.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27401..27575)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27748..28185
                     /gene="ndk"
                     /locus_tag="ACG8NF_001008"
     CDS             27748..28185
                     /gene="ndk"
                     /locus_tag="ACG8NF_001008"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0471505.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28207..29055)
                     /locus_tag="ACG8NF_001009"
     CDS             complement(28207..29055)
                     /locus_tag="ACG8NF_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0471506.1"
                     /translation="MKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSFVTLRSF
                     LERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHFKKTEDL
                     YYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFPWIFSDR
                     FIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNEDMKRSLA
                     HKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSLEKKLLA
                     IAEPSN"
BASE COUNT         8388 a         6101 c         5162 g         9406 t
ORIGIN      
        1 aatttattgc ccgataatat atagaaataa ggtctagtta ctgttttttt taaatgatta
       61 ataaataaca acttctttct atgaaattgc aaagcattca tcttcttttc cgctaacgat
      121 atatccgtca gtgcttggta cactttacta aaaagtatga gattgccgat atctggatag
      181 gtgtgctata ctacagaaaa agatatacaa tctgccaccg ggtaggataa agaggatgat
      241 cattccatta ggtctttgaa gggatgattt tcctcttttt gtgtattttt ttatagttaa
      301 ggaggaatgt aagtatggca tggatggaat taatcatagc aggaattttg gaagtattct
      361 ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag
      421 tgattggcat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga
      481 gtctctctta tcctatctgg acagggattg gcgcagttgg ttcggtcata attggtgtgg
      541 tcttgtttca tgataagctg aatctttcca cttggttctt tgtggggatg ctactcatca
      601 gtatcattgg catcaagatt actagcggcc actagacact tggaataagt actgatcaga
      661 aaaaataagc ctttttattt aatattcaat ttttttatga aaaaataaga aataagtcca
      721 atgacctagc gacttctcca ctcatcaggt gtatgcttgt actatctata gaaaaggagt
      781 gaatattttt gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag
      841 ccctcagcga tgtcagtttt agcttatccc ctggcaaaat cgttggatta gtaggtccta
      901 atggcgctgg aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagatg
      961 gaacaatctg tattggtgaa aatgagattt ctccctctag tcataaaggg ttggaacaag
     1021 taggagcatt gatcgaatac ccgggtatct atccattctt aacaggctat gatcatctga
     1081 agttattctc tgaaaccaac gatgtatctg ctatcgacac tatcgtgaat cagctcaaga
     1141 tggaaaagta cattcacaaa aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg
     1201 gaattgctct tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg
     1261 gtttggatcc acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg
     1321 gcacctcttt cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc
     1381 tgatcctgat caatcaagga aagatcatca aacaatgtac aatggctgaa ttgaaccaaa
     1441 cagcgcaaga tttcatcgtt atctctacaa caaatgatct agctgctcga acgatcttga
     1501 cagaagctgg atatactttg gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag
     1561 aattattagg aaccgtgatc cagctattgt ctgcacaaaa tatccatgtg acagacgtcc
     1621 agcatctgac gaatgatttg gaatcttctc tattggaaat tctggattca gtaaaggggg
     1681 aagaagaaaa atgacattgc ttgtaaaaca agaattattt aaattgatca agaaaaagtc
     1741 aactgctgtt ttatctgttc tattagttgt tttgctcata ggaacagcac ttctagccaa
     1801 aaaatacacg acgatcattg atccagttga aatgactgct cagcttttta gtgccacatc
     1861 ttggattgtc tttatcatga ttgcagctgc aagcacgatc atttcgatgg aagcacaata
     1921 tggtacattg aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagcaa
     1981 atggatcaca ttagttattt attctgttta tttatacctt ttagctatta ttgtcactgt
     2041 gttgatgaaa ctgattcttt tcccttcgat ttccttcacg gagaaggttt ctactggtca
     2101 aacactgatc caaagcttgt tcacttatac tttgggtagt tatatcggtt tatggctgat
     2161 tttaagttta gtgttgatga ttgcttgctt tatcaatagt tccggcgcat ccatctcagc
     2221 aggaatcgtc ttttattttg cttcgtcaat catatctggt atccttattg cactcatcca
     2281 aaaatgggag tggatcaaat ggaatccgat cagtatgttg aatctgcaaa accaaattgg
     2341 aaacgaagag atcatgaagc aattgactca tctttctact aatcaaatgc tattcggtaa
     2401 tttagcttac atcgttttgt tcttagcatt aggatatctc gtatttaagc gaaaaaatgt
     2461 atgatgactc atcaaaaaag aggctgtgaa agagacgttc agccttattg ccgattggct
     2521 gtctcttaaa caccgtttat tctgaaaaag gagctgtgac aacatttgtg tcacagctcc
     2581 tttcctagta tgtttttgaa taaacgacag gctcacaacc tctctttata cttcttttat
     2641 acttctatac gtgaagcatc ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg
     2701 tcacacctat aagtaaaatc ttatctaagt ggatatgttg tacaggctcg ttttgctcct
     2761 tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag aacaagaaca agcattactt
     2821 cattaaaggc tacgactagc atagcccata cttgaaaggc acttgctaaa agccctacta
     2881 aaaattggac ccattctttc cgctgataag aaagtttcat ttggtacaat cctaagaaaa
     2941 gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag
     3001 tgaataataa tgaaagtaaa aagacattcg tacataacgt cgtcaatacg atagcataag
     3061 ttggtgcccc atttttgttg agttttcccc atgctttagg caaaagtccg tcttcagcca
     3121 tgtttcccat tgtttcaatc ggtaagatcg tccatgagag ccaaacacca actaaagaaa
     3181 tgatcaaacc gccgttgatc acgactgctc cccatttacc aaccatcatt tcaaacacat
     3241 agcccatagc aggctgcgcc ttgatcgttt gcagctgatt catcgtcatt gtcccatatg
     3301 gaagcatgga aaccaagacg taaataataa tcaagccgaa tatccctaga atcgttgctt
     3361 tcccaacatc tgattgtttt tttgctcgtg aacctacgac agaggctcct tcaataccaa
     3421 caaataccca aagcatcacc atgatgcaac tcaatacttg actggaaaac gggatcggct
     3481 gtccattcgc attcaatata atgtcgctag aaaagttatc aatgaattgt tggatcgaaa
     3541 acgtgctgat agctactaca ataaataaaa ataatggcac taatttacag aatgtgacga
     3601 tagcattgac gaaactagca ttttcgactc cccgattcac tagccatgca tatccccata
     3661 aaaagactgt actcatcacg atcgatgcca gattttgccc accttcaaat actgggaaga
     3721 aatatcctaa tgcgctcatc aacaatgttg caaaagctac gttgcctaac cagcatgtga
     3781 gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac
     3841 taaagatccc gccttttaaa tctggacgtt ttttattgat attattgatc gacaaggaga
     3901 gcatcaacat tgctgttcct acgagtaccc aactgaaaat agctgggccg ccagccgatc
     3961 cggagatgtc cgtcataata ccaaatacgc cacctccaat cgctgaacca attactaagg
     4021 cagcaagtgg aattaaacca attccttttt tctgttgctc tgtatccatg taagcatcct
     4081 ccaaaataaa accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta
     4141 ataaaaactt taatagaaat ataattctac cggctttttt ctcattatac aagcaaagcg
     4201 cctagtccct ttccaaatta caccaccaaa aaacttaaaa aagatgctat actaaaagga
     4261 aagaattgga ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc
     4321 ctttgccaaa catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc
     4381 tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt
     4441 ttttactttc gttcctactt atttagacgg cggaaatggt ttagataacg gtgatacaga
     4501 aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa gacaatcata aactttgtct
     4561 tggtgtagtc ggtagcggaa ataaaaactt caataatcag tattgtctaa cagccaaaca
     4621 atatgcacaa acatttggct ttcctttctt ggcagattat gaattacggg gaacaccatc
     4681 tgatgtgaca cgaatttatc agattttagc agaaaacaat aaataataaa aaagaagaag
     4741 ctgaaaaata gatttcagtt tcttctttcg tttctattgt ttctatcata ttaagctttg
     4801 acatccatca caggtgcttt tgcttttgct ttacctgttt tttccaagac aaagtttttt
     4861 acacgatcca tattggttac caagacgatc atggatgttt ctttgcctgc tgctttgatc
     4921 gcttctaagt cagcttctgc tacaagcgta tcaggtgtca cttgctgccc ttctgttact
     4981 ttcacatcaa atggcgtacc gcctaagtca actgtgttga ttcccatatg aagcaagatt
     5041 tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg ttgggaagac agagctgact
     5101 accccagtaa ctggagaata gatatgtccg ttttctggtt ctacaaagta gccttcaccc
     5161 atcatctttt gtgcaaaaac tggatcactt gctttatcta aagaaacaac tgttccgtca
     5221 gcaactgatg agaggatagt cacactttca gctgaagttt gaggtactac tttttcaggc
     5281 acttcattta ctgtggctgt ttcagtcgct aattcttctt tttctaaacg gtctttagga
     5341 ataccgaaga agaatgtagc aacgaagcca ccagcataag ctgccaataa tccaaggatg
     5401 tagcctaacc atttaccgtc agagatcaat gggatcaaag cggctcctga aggtccaata
     5461 gcgatagccc cgacatttcc gatcattccg atcactgccc cgccgatacc tccaccgata
     5521 caagcagtaa taaatgggcg tcctagaggt aatgttactc cgtaaatcaa tggttcgccg
     5581 attcctagga tcccgactgg taaagctcct ttgatcattt ctgttaattc tttatctttt
     5641 ttgcagcgaa tccataatgc caatgctgca cccacttgtc ctgcaccagc catcgcaaga
     5701 attggaagca atagcgtacg accagtttca tcaatcattt gcacatggat tggagtcaaa
     5761 atttgatgca gtccgaacat aaccatcggc aagaataatg tgcctagaac gaagccggca
     5821 aatgctccac caacgccaag tacccagttg atcccaccga ccatgccatc agaaatgaaa
     5881 cctgctaaag gcatgatgaa gaaaatttca atcagaccga tggcaatcaa tgaaagagtt
     5941 ggtgtaacga tgatatctac cgcatcaggt actactttat gcagtttttt ctcaacgatt
     6001 gacaatagcc aaacagcgaa gatgacacct aggatcccac cttgtccagc tgcaagagct
     6061 tcaccagtaa ataagttttt cagtggtgct tccggattca tccctgtcag catcacaacg
     6121 gccccgataa ctccaccaag agttggtgtt gcaccaaaaa cttgtgcagc attgatacct
     6181 gtatagataa ctaggtagct aaacatcccg tttttcaaga tgttcatcac atcgatatat
     6241 tgttgccatg ttgctgcatc aagtgttccg gctgtgacta agtttgctaa aactgcggca
     6301 atccctgcca cgatcccagt accgacaaat gcaggaatca ttggcacaaa gatattagaa
     6361 atgtctttta ataccgattt gattttagat ggtttttgtt tcgatttttg tgccgctttc
     6421 atttcttgtg cccgttcgtt tacacggtct tttccagatg ctgatgaatt tgctgtagct
     6481 gtacttacag aagaaagttt ttctcctaat ttcacgcctg ccaaatcaac catttgctgt
     6541 gctactttgt tcactttccc aggtccgatg atgacttgaa gctgttcatc ttctactaca
     6601 ccaagcactc ctggaatcgc ttttaattca gagacattta caagatcatc attgatgatc
     6661 ttcatacgca cacgagtcat gcagtggaca atgctttcta cattgcccat gccgcctgcc
     6721 cctttataaa tttgttttgc cagttcttcc aaagtcagtt cgttttttgc catggtcttt
     6781 tccccctcat aaggtccctt ttacaaatcc gtttgcttca gctaattttg cttgagcttc
     6841 ctctttatca ctatttgtca aaatcatgac gatagccagc ttaacatttt gatctgcttc
     6901 ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc gcttgcatga tgattcgttt
     6961 tgagcgttcg attagttttt catttgttgg tttgacatcg accatcaagt tattgaatac
     7021 tttacctata ccaatcatac tgatagtaga aatcatattc aagataagtt tttgtgctgt
     7081 tcctgacttc aatcgagtag agcctgtcaa aaattctgga cctgcatcga cttcgattgg
     7141 gaaatccgcg tgtttactga tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc
     7201 accgatcgat tttgcgtaat ctaatccacc gatcacatat ggtgtacgac cgctagctgc
     7261 gataccaata actatatcat tttttgtcaa attcagcttg attagatctt cttgccctag
     7321 ctcataagaa tcttcggccc cttcgacagc tacagtcatt gcttcttctc caccagcaat
     7381 tagacctatg accatttccg gttctacacc gaatgtcggt acgcattcag cagcatctaa
     7441 cacacctagt cttccacttg ttccggctcc catataaatc agacgtccac cttttttaaa
     7501 ggactcaatc gtgttagcaa tcactggttc gatacttgtc agttcttttt caaccgcagc
     7561 ggccactttc tgatcttctt tgttcattag ttgaacggct tgagaaatac tcatctcatc
     7621 taatccaaaa gtttcttggt ttctgcgttc cgttgttagg ttctctaaat tgattttgct
     7681 catttttatc ttccttcttt tcttataaat tgatagttca taccagcttt gatttggttg
     7741 atcaatggta gctcttcttt gataacttta gctactacat tcactttttc atccgctggc
     7801 aaatcatatt tggttaattg gatctcaccc atatagcgca aatacttttc gttatctaaa
     7861 gtaatagacc ctttcatacg cgtagctgta tttcttgctg ggataggcgg aatctctcga
     7921 aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt cttgccgatt cgtatgcttc
     7981 cctaatatat attcgtaaaa ttcttcatct acaggttccg tatggagcaa gactgtctgt
     8041 tctttttgga aagaagcaaa ttgctcctga acgttttctg acagacctgc atctccgatg
     8101 taaacaatat ccgtgttact tgctaataga tctagagcgc ctgcaagagg atggatccct
     8161 cgatgttctt ccaaagttgg caatccttga tatagtgggc ctctcaactc tgtatctcct
     8221 ggtacaaaac cttggacaag gagaccttga attttgagcc attgattttt tttctgatac
     8281 cattctttgt ccagccctgt ttcgggacgt ggataataat tatgccaagc ttcaatctgt
     8341 gagaaatcag cttctgcttc tttcaattca tcgatatctt taggcgtgat cgtgctagca
     8401 ttcaaactga ttttcaattg atgtgaccag tcggcaatct gctgattgga aatatgataa
     8461 tccattctta aacctgttac accaattgct ttcaaaggcg ttagatcttc taatgaaaaa
     8521 cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca attctagttt attctttttc
     8581 gcttcgcttc ctagttcgat caatcgcttt tgatatgccg aaacatcatc ttctggtata
     8641 tgcatggaag tgaaaatacc gacaaagcca ttggatgcca tttggcggat atactgtttc
     8701 ttctcttctg acaaagaatc gttcataaat attgaaaagc caaacatatt atccctcttt
     8761 ttctgtatcc gtcttcattt ctatgactag agtatatatc taaagaaaca aaatttcaat
     8821 acttttatta aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat
     8881 tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa
     8941 ataacacaaa aacataatta ctcataagaa accaattctg tattatctac catgaatgta
     9001 aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta
     9061 tggcaaacta acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg
     9121 atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa
     9181 cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt
     9241 tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct
     9301 gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct
     9361 actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct
     9421 gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa
     9481 agcatgtgat tctctccttc gttggcaaag ttccccttaa ccataccttg ttgttcttga
     9541 gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag
     9601 gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat
     9661 tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct
     9721 ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact catctgctcg
     9781 atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct
     9841 tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa
     9901 ttttggcttg cataactaat gctcagatgt tttggctggt atttttctcc tataaccttg
     9961 ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct
    10021 gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt
    10081 tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta
    10141 ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc
    10201 tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca
    10261 cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt
    10321 gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc
    10381 ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt
    10441 ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt
    10501 gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag
    10561 ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga
    10621 ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg
    10681 acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct
    10741 gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt
    10801 aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct
    10861 tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt
    10921 gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga
    10981 tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt
    11041 tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg
    11101 ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc
    11161 caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct
    11221 ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat
    11281 tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc
    11341 taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt
    11401 ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca gaaagtcctt
    11461 ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa
    11521 gcactgctac accgtatgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc
    11581 ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta
    11641 aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg
    11701 ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag
    11761 caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc
    11821 ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga
    11881 taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag
    11941 ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc
    12001 ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt
    12061 gaagcagtgc atctttaata agttctccta aggcataact ggaagcaagt cggatagttt
    12121 gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg
    12181 tgttttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataagagat
    12241 ccatcccatc aatggtacgc catgttcccc tgagcgcgta atagattgct tctgcagtat
    12301 tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat
    12361 ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg
    12421 ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt
    12481 ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga
    12541 ataaaggaga gaaaaaacat gaaaatcgca atagctgggg ccggagcaat gggcagccga
    12601 atcggattga tgctacatca aagcggaaat gaggtcctct tgatcgatcg ttggcctgct
    12661 cacattgaag ccattcgtac aaatgggttg attgctgact ttaacggtaa agaagtggtc
    12721 gcaaaactgc caatctattc accagaagaa atcatcgaaa gcaacgaaca cgtggacttg
    12781 atcgtagcat taacaaaagc caatcaatta gatgatatgt tttgttcgat ccaatccatt
    12841 attactgaca atacttatgt tttgtgttta ctaaatggat taggtcatga agatgtactg
    12901 gaaaaatatg tgccaaagaa aaatatctta tttgggatca cgatgtggac agctggtcta
    12961 gctggtcccg gaaaagtcac attgttaggt gatggagaaa tcgaactaga gaacttggaa
    13021 ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat
    13081 ccgatctata gccataacgt tcgttattcc atttggcgaa aagcttgtgt aaatggaaca
    13141 ttgaatggtt tatgcacgat tttggattgt aatattgctg aactaggcgc gcaaaaagcg
    13201 gcagagagca tggttcgaac aatcgtatct gaatttgctt ctatcgcagc aaaagaagga
    13261 atcatactag atcaagaaga agtgtaccaa catattgctt ctacttatga tcctgacaac
    13321 atcgggttgc attacccatc tatgtaccaa gacctgatca aaaaccatcg tttgactgaa
    13381 atcgactaca tcaatggtgc catttggcga aaaggacaaa aatatgatat tgctacacca
    13441 tattgtgcct ttttgaccca attagtccat gcaaaagaag gaatccttgg cgcagaataa
    13501 aaggctctct gatgagaatc agctgaatca gcagctggtt ctcttttttt aagcccaata
    13561 aaagatcaaa aagaaaacac aactctcatg atgccataag aattgtgctt gtttgtttca
    13621 gtacataatc tcttcaaaat gtaggttttt tatttgtttt caacagagga gaggtcatat
    13681 tcgtattgct cataaatagt ggaatcgtta gtgataaatc gttttgccca attttctgca
    13741 tcataaaatt ccaatagctc tgataggatt ggcttaaatg gtgtatgcag aaattccggt
    13801 aattcatcta aatcacgttc tcgaagcaaa aaaatatttt caggacgata aaaatgataa
    13861 tgagcaatcg cttgattatc cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc
    13921 cgcaaagtca ctccttgctg accctcccgc aacagttcca gaatcccgcg cgattcccct
    13981 gtttttttca ggtactcttc ataagtaaga tatccaggtt gttcattccc tcgatgtgga
    14041 agaatgaaat agttggtcgt aacatctctt tttataaatt cctgcttata gctttctgct
    14101 cgttcttttc tcccattatt cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa
    14161 gaaaaattca tatgtttcga ataaaatgaa ctattatgtt tcagcccaag ttcttttgct
    14221 tccaaaaaat caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc
    14281 atcgcctcgt gttctgcttt gaagtgattc caaaaaaata acacaatttt tgcattggta
    14341 aatgggcgca cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc
    14401 tgataatttg ccaactgttg tctagctgat ttaatgaatt ccgactgtag taaatctttt
    14461 ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat aaagtgattt attttttgca
    14521 actagcagta cgtcattttt gttcatcttt tccgcctcac tttctctgtc actttttcct
    14581 atgatagcct gtttaaatag taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat
    14641 ttaacatttg tatgaaaata tgatgaaata aatcattctt ctattaaaat tcgcctaaaa
    14701 gttttctgaa tagttgtaat tgtctgaaaa tttgttatca ttagtcattg ttaaatattg
    14761 ctatttattc ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa
    14821 gataaaaaat tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga
    14881 gcaatcggtg caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct
    14941 gtattgattg cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga
    15001 aaaatcgttt taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat
    15061 ctaggtgctc attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat
    15121 atcatcgctg aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca
    15181 gcctgggtat ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta
    15241 cggttattcg cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta
    15301 ctgatcattt ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct
    15361 acccttcagc aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc
    15421 agttctttgc ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta
    15481 agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga
    15541 atcatttcct tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact
    15601 ttagcaggca cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt
    15661 gcagcggata tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt
    15721 gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga
    15781 aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt
    15841 gttctttatc ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg
    15901 gcaggttccc tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt
    15961 gtactggcac taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct
    16021 gtcttaggat tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg
    16081 actggcctgc tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta
    16141 ttgacgaatg aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct
    16201 attgaagaaa ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc
    16261 tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc
    16321 catgctcatg acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa
    16381 gtattggaag tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta
    16441 cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt
    16501 gatgatgggt gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa
    16561 caaaaagata aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt
    16621 tatggtacga agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca
    16681 tcattctatc gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa
    16741 attagcggaa gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat
    16801 caaagaaatg gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat
    16861 cagcttcaag aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa
    16921 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt
    16981 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa
    17041 aatcagcgtt ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg
    17101 ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg
    17161 aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt
    17221 gtataaattt ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg
    17281 attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt
    17341 cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc
    17401 actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag
    17461 tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta
    17521 gttgattact gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc
    17581 tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc
    17641 aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt
    17701 cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa
    17761 tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc
    17821 tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc
    17881 gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac
    17941 tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag
    18001 taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac
    18061 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
    18121 gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg
    18181 tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga
    18241 catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa
    18301 gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
    18361 actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg
    18421 gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca
    18481 tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat
    18541 gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa
    18601 catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac
    18661 gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg
    18721 tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
    18781 ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa
    18841 agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    18901 tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    18961 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg
    19021 tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    19081 atattttttc atttttctga aaattatttt tataactttc acacacaaga ttatttttta
    19141 ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat
    19201 tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc
    19261 tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt
    19321 gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg
    19381 gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc
    19441 atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac
    19501 gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat
    19561 cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt
    19621 tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc
    19681 tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt
    19741 gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc
    19801 tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc
    19861 tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa
    19921 aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg
    19981 ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc
    20041 ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa
    20101 agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc
    20161 atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa
    20221 ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg
    20281 gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg
    20341 gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt
    20401 tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt
    20461 gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc
    20521 ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa
    20581 gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac
    20641 tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc
    20701 tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc
    20761 cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc
    20821 attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga
    20881 ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc
    20941 tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc
    21001 tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt
    21061 ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc
    21121 tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc
    21181 caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc
    21241 tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa
    21301 aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat
    21361 accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag
    21421 tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt
    21481 ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt
    21541 cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa
    21601 caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga
    21661 aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat
    21721 tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg
    21781 tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa
    21841 aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat
    21901 tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata
    21961 cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa
    22021 tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac
    22081 ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg
    22141 ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact taattccacc
    22201 agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct
    22261 tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg
    22321 aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg
    22381 ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac
    22441 tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca
    22501 ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct
    22561 actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa
    22621 ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa
    22681 tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc
    22741 tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc
    22801 agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca
    22861 cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt
    22921 tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat
    22981 acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca
    23041 agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca
    23101 agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg
    23161 cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata
    23221 gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact
    23281 taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc
    23341 agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc
    23401 aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct
    23461 acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac
    23521 cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt
    23581 ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta
    23641 ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc
    23701 ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa
    23761 actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac
    23821 taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt
    23881 cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc
    23941 caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt
    24001 cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata
    24061 tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc
    24121 tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg
    24181 tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg
    24241 ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc
    24301 taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt
    24361 ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt
    24421 tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg
    24481 actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca
    24541 ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac
    24601 atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc
    24661 actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa
    24721 atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata
    24781 acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa
    24841 accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta
    24901 tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa
    24961 agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac
    25021 atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt
    25081 tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg
    25141 tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact
    25201 ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta
    25261 cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg
    25321 tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc
    25381 aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc
    25441 ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt
    25501 gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct
    25561 ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt
    25621 aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt
    25681 aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc
    25741 gcaccttttg tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga
    25801 cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct
    25861 aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga
    25921 tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc
    25981 atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac
    26041 acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg
    26101 gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt
    26161 gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta
    26221 agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg
    26281 gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct
    26341 gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca
    26401 ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg
    26461 tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg
    26521 gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct
    26581 gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca
    26641 ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg
    26701 gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg
    26761 gctacaccga aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa
    26821 ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct
    26881 acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa
    26941 cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct
    27001 tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc
    27061 tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt
    27121 catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc
    27181 ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac
    27241 taagtaattt taattcatga cttccaatca tttttcgaat agttattttt ccaattagac
    27301 cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa
    27361 tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag
    27421 ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct
    27481 tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta
    27541 tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa
    27601 ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca
    27661 cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag
    27721 aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac
    27781 ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc
    27841 attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac
    27901 ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt
    27961 tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca
    28021 aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa
    28081 aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc
    28141 ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact
    28201 gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg
    28261 attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc
    28321 ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat
    28381 cactgtactt ctttctttat gtgctaagct ccgtttcata tcttcattca cactacgtgc
    28441 ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag
    28501 atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata
    28561 attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc
    28621 aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg
    28681 taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac
    28741 caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata
    28801 aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat
    28861 tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc
    28921 taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac
    28981 tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac
    29041 tcgaagtttc ttgatca
//
LOCUS       DAZKOM010000022        28603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000022 DAZKOM010000000
VERSION     DAZKOM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.022"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG8NF_001010"
     CDS             237..1877
                     /locus_tag="ACG8NF_001010"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0471507.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG8NF_001011"
     CDS             2031..3353
                     /locus_tag="ACG8NF_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0471508.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG8NF_001012"
     CDS             complement(3415..3846)
                     /locus_tag="ACG8NF_001012"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0471509.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG8NF_001013"
     CDS             complement(3910..4746)
                     /locus_tag="ACG8NF_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0471510.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG8NF_001014"
     CDS             complement(4936..5238)
                     /locus_tag="ACG8NF_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0471511.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8NF_001015"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8NF_001015"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0471512.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG8NF_001016"
     CDS             complement(5692..5958)
                     /locus_tag="ACG8NF_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0471513.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG8NF_001017"
     CDS             complement(6212..6937)
                     /locus_tag="ACG8NF_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0471514.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG8NF_001018"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG8NF_001018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG8NF_001019"
     CDS             7490..8242
                     /locus_tag="ACG8NF_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0471515.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG8NF_001020"
     CDS             8258..8737
                     /locus_tag="ACG8NF_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0471516.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG8NF_001021"
     CDS             complement(8988..10568)
                     /locus_tag="ACG8NF_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0471517.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG8NF_001022"
     CDS             complement(10561..11304)
                     /locus_tag="ACG8NF_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471518.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACG8NF_001023"
     CDS             complement(11480..12256)
                     /locus_tag="ACG8NF_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0471519.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACG8NF_001024"
     CDS             complement(12504..13340)
                     /locus_tag="ACG8NF_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0471520.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACG8NF_001025"
     CDS             complement(13351..14280)
                     /locus_tag="ACG8NF_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0471521.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACG8NF_001026"
     CDS             14472..14825
                     /locus_tag="ACG8NF_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0471522.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACG8NF_001027"
     CDS             complement(14899..15555)
                     /locus_tag="ACG8NF_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0471523.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACG8NF_001028"
     CDS             15781..17715
                     /locus_tag="ACG8NF_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0471524.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACG8NF_001029"
     CDS             complement(17801..19024)
                     /locus_tag="ACG8NF_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0471525.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACG8NF_001030"
     CDS             complement(19018..20889)
                     /locus_tag="ACG8NF_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0471526.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACG8NF_001031"
     CDS             complement(20910..22028)
                     /locus_tag="ACG8NF_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0471527.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACG8NF_001032"
     CDS             complement(22111..22944)
                     /locus_tag="ACG8NF_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471528.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACG8NF_001033"
     CDS             complement(22937..23854)
                     /locus_tag="ACG8NF_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471529.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACG8NF_001034"
     CDS             complement(24081..25205)
                     /locus_tag="ACG8NF_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0471530.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="ACG8NF_001035"
     CDS             25388..26296
                     /locus_tag="ACG8NF_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0471531.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="ACG8NF_001036"
     CDS             26437..28050
                     /locus_tag="ACG8NF_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0471532.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DAZKOM010000023        27847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000023 DAZKOM010000000
VERSION     DAZKOM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.023"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            854..2023
                     /locus_tag="ACG8NF_001037"
     CDS             854..2023
                     /locus_tag="ACG8NF_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0471533.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            2496..3050
                     /locus_tag="ACG8NF_001038"
     CDS             2496..3050
                     /locus_tag="ACG8NF_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0471534.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(3223..3660)
                     /locus_tag="ACG8NF_001039"
     CDS             complement(3223..3660)
                     /locus_tag="ACG8NF_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0471535.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3708..4655
                     /locus_tag="ACG8NF_001040"
                     /pseudo
     CDS             3708..4655
                     /locus_tag="ACG8NF_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4926..5361
                     /locus_tag="ACG8NF_001041"
                     /pseudo
     CDS             4926..5361
                     /locus_tag="ACG8NF_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            5377..6210
                     /locus_tag="ACG8NF_001042"
     CDS             5377..6210
                     /locus_tag="ACG8NF_001042"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0471536.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            6455..7480
                     /locus_tag="ACG8NF_001043"
     CDS             6455..7480
                     /locus_tag="ACG8NF_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0471537.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7618..7743
                     /locus_tag="ACG8NF_001044"
     CDS             7618..7743
                     /locus_tag="ACG8NF_001044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471538.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7768..8883
                     /locus_tag="ACG8NF_001045"
                     /pseudo
     CDS             7768..8883
                     /locus_tag="ACG8NF_001045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8966..9862
                     /locus_tag="ACG8NF_001046"
                     /pseudo
     CDS             8966..9862
                     /locus_tag="ACG8NF_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9878..10702
                     /locus_tag="ACG8NF_001047"
     CDS             9878..10702
                     /locus_tag="ACG8NF_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471539.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10734..12044
                     /locus_tag="ACG8NF_001048"
     CDS             10734..12044
                     /locus_tag="ACG8NF_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0471540.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12192..13451
                     /locus_tag="ACG8NF_001049"
     CDS             12192..13451
                     /locus_tag="ACG8NF_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0471541.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            13461..14333
                     /locus_tag="ACG8NF_001050"
     CDS             13461..14333
                     /locus_tag="ACG8NF_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471542.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            14345..15175
                     /locus_tag="ACG8NF_001051"
     CDS             14345..15175
                     /locus_tag="ACG8NF_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471543.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            15215..17398
                     /locus_tag="ACG8NF_001052"
     CDS             15215..17398
                     /locus_tag="ACG8NF_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0471544.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            17459..18679
                     /locus_tag="ACG8NF_001053"
     CDS             17459..18679
                     /locus_tag="ACG8NF_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0471545.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18676..20136
                     /gene="gtfA"
                     /locus_tag="ACG8NF_001054"
     CDS             18676..20136
                     /gene="gtfA"
                     /locus_tag="ACG8NF_001054"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0471546.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            20213..21319
                     /locus_tag="ACG8NF_001055"
                     /pseudo
     CDS             20213..21319
                     /locus_tag="ACG8NF_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21641..21850
                     /locus_tag="ACG8NF_001056"
     CDS             21641..21850
                     /locus_tag="ACG8NF_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0471547.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21843..21992
                     /locus_tag="ACG8NF_001057"
     CDS             21843..21992
                     /locus_tag="ACG8NF_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0471548.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22146..22811
                     /locus_tag="ACG8NF_001058"
     CDS             22146..22811
                     /locus_tag="ACG8NF_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0471549.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23095..24180)
                     /gene="xerS"
                     /locus_tag="ACG8NF_001059"
     CDS             complement(23095..24180)
                     /gene="xerS"
                     /locus_tag="ACG8NF_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0471550.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            24514..24747
                     /locus_tag="ACG8NF_001060"
     CDS             24514..24747
                     /locus_tag="ACG8NF_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0471551.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24744..25151
                     /locus_tag="ACG8NF_001061"
     CDS             24744..25151
                     /locus_tag="ACG8NF_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0471552.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26288..26806)
                     /locus_tag="ACG8NF_001062"
     CDS             complement(26288..26806)
                     /locus_tag="ACG8NF_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0471553.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27173..27421
                     /locus_tag="ACG8NF_001063"
     CDS             27173..27421
                     /locus_tag="ACG8NF_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471554.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9536 a         4217 c         5099 g         8995 t
ORIGIN      
        1 aattttctac gttttccttt tcatatatat taaatattac ggaaaattat ataaactaat
       61 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
      121 taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc tttcgtactt
      181 aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata
      241 tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct
      301 ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta
      361 tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga
      421 ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt gataataaag
      481 tactacctat taaaggtagt actttattta atttagtata aattaacata ctactatatt
      541 ttaggtttca tattatataa attaataata tactaccatt tgttggtagt ataatttaaa
      601 ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa gtttaataag
      661 agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat
      721 aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta
      781 gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata
      841 ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg
      901 tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc
      961 tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa
     1021 ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt
     1081 aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac
     1141 caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga
     1201 aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa
     1261 tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag
     1321 atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa
     1381 agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt
     1441 tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga
     1501 ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag
     1561 tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa
     1621 tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt
     1681 ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga tagataaatg
     1741 gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat taacctttta
     1801 taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg aaggttttaa
     1861 caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag aatctctgga
     1921 gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc gagttcataa
     1981 aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc aacagaatgg
     2041 gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct tgcgtaaagt
     2101 tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg ttattcatat
     2161 gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa acttaaattt
     2221 catataattt aattatgtaa attagctatt ctaagaactt ctacggataa ctgcccgtct
     2281 aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt tgtacaaaaa
     2341 agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc ccttgatttt
     2401 attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat atacataatc
     2461 attaactcag aatattttta agaagggaaa caatagtgag ctataatgta caaccgttaa
     2521 ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt aatccggaaa
     2581 gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca gatattatag
     2641 gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa cataaaacag
     2701 gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta tatactgaag
     2761 gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat ataacaaggg
     2821 atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat gatataggaa
     2881 cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga gacattgcta
     2941 ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac attggtataa
     3001 ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa tttaaaaatt
     3061 tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat tttatttcat
     3121 aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt gttatataaa
     3181 ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa agagaatcaa
     3241 tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact caacgtttag
     3301 cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt tgaccttctg
     3361 caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc
     3421 ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt taatatctgt
     3481 atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga gcagactaca
     3541 atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt taatgatgat
     3601 tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg atgaaaacaa
     3661 aagttgttca ccatctttta taggtttgat tctcattttt agatattatg cccgcacgta
     3721 ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt tatcataata
     3781 tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc
     3841 tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt gaagtacaag
     3901 aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta aaagttcctt
     3961 ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt tgccattatg
     4021 gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat gcaacacgat
     4081 tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg tatgtcacag
     4141 accagatccc tacagagcat gccattttag gaattgatgc cattcaacta cgcgtacaat
     4201 attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa gaatacgtca
     4261 gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact cagttctatc
     4321 aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg attggtagcg
     4381 tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc gacaaacaat
     4441 tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt gtatcacttt
     4501 attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga aacctagatg
     4561 caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt ttaccgcagt
     4621 acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc catgacgaag
     4681 agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag
     4741 aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta ataaaactac
     4801 ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt aaactaaaca
     4861 tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat aaaggggagt
     4921 accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa aaatgtcagt
     4981 ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc aggaaaatca
     5041 tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga cacctatgat
     5101 agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga gattgataat
     5161 ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta
     5221 gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct
     5281 agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg ttctgtctgt
     5341 cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc ggctattgat
     5401 aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg aattgaggaa
     5461 caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc agaagatgtt
     5521 aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa
     5581 gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga tacgcatctt
     5641 tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt aaatacaaat
     5701 gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca tgagaaaata
     5761 gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga acgacatcaa
     5821 gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg gatatataac
     5881 aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat tgttgttact
     5941 ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa acgagtgatg
     6001 gaacaattat cgattattgg atttgatgat attacttttg cagaattaat ggatcctgcg
     6061 ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca ccaactgatt
     6121 ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg agaattgatt
     6181 tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt atagttaaca
     6241 ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt
     6301 cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt ttttaattat
     6361 gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga ttatatgtta
     6421 gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg atgttgcaaa
     6481 acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc cgacactttc
     6541 agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc attatgtcaa
     6601 aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag tttctgaagt
     6661 agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag tcgaagcaga
     6721 acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt tggagcaaga
     6781 cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt ctgtgattga
     6841 agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta ttgaagctga
     6901 agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag atcgattgta
     6961 taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt tagatagcca
     7021 aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga cgtggatgaa
     7081 acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca cacttgacgg
     7141 aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc cgactgctat
     7201 catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct
     7261 acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg ctgaattttt
     7321 aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac tagcagttcg
     7381 catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga ttgtttctaa
     7441 taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa
     7501 gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa aacattaagt
     7561 aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg aattaagatg
     7621 aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac attactcgca
     7681 gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag ctatgcgagt
     7741 tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga aaaacaaaat
     7801 cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt taaattagaa
     7861 gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag cgaatcctat
     7921 aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga aaatgatatt
     7981 gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga cgaactatac
     8041 gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt atttgataat
     8101 gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca agctgcaatt
     8161 gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg aacagagact
     8221 ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga caatacaaaa
     8281 tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga tattgcactt
     8341 gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt aagtcgtttt
     8401 caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg atttttgaaa
     8461 aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa agttaatgca
     8521 acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc agaagaagtg
     8581 aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc ggaaacgggg
     8641 gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc agatacaatt
     8701 gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata caataaagat
     8761 ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc tggtgaagta
     8821 actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt gagcggaaat
     8881 taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg tatattaatg
     8941 gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat atcgtaaaga
     9001 tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa ttttattaaa
     9061 tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag ggttcggtga
     9121 tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg aagtttttca
     9181 gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg ttgtattatc
     9241 cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt atcggacaat
     9301 ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc gctggatgtt
     9361 caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg atggaccagc
     9421 atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag tttggagtgc
     9481 tattggttat aacatggtgt tattactagc cggcttataa gaaattccta aagattatta
     9541 tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca ttacgttacc
     9601 attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg ctttccaagt
     9661 atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta aatcacaatc
     9721 attagcctat ttattctata aacattcatt tattttaaat gacaagggat atggttcggc
     9781 aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa tgaagttgca
     9841 gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat ttttttcaaa
     9901 tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac cttttatttg
     9961 gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac ctacaatttt
    10021 ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac cattcatggc
    10081 tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg ttttattttc
    10141 agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata tccttttcag
    10201 cttagtactt attcaaatga tgattcccgg ttccattttt attactccac agtatttatt
    10261 agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag gggtagttag
    10321 tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg aattggagga
    10381 agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta tgttgccgct
    10441 tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat ttaaagattt
    10501 aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc cgggattagc
    10561 aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt cgttattagc
    10621 aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg aaggaattgc
    10681 tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag tacatgacaa
    10741 aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga
    10801 tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat gaaaaacgac
    10861 taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg
    10921 atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt gttatcaatg
    10981 ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt cctaaaaaat
    11041 atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt agaaccctac
    11101 ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt ccaaatgctt
    11161 ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta aaagcgacga
    11221 gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc
    11281 atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt gcaggcatta
    11341 atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat ccaacaattc
    11401 gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt gaattgttga
    11461 aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac catccatact
    11521 ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg gatgaatact
    11581 tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac attgtaaata
    11641 atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg gattctatta
    11701 cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta atcacaaact
    11761 taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa
    11821 cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat atcaatgttc
    11881 aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat caagcagcgt
    11941 atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat ttagtggatg
    12001 atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa gctgaaagag
    12061 taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat attgacaaaa
    12121 cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg
    12181 aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa
    12241 cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag
    12301 agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg
    12361 aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa
    12421 ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta
    12481 tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt
    12541 tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca
    12601 taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg
    12661 ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc
    12721 aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc
    12781 aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct
    12841 cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag
    12901 atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata
    12961 cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc
    13021 cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg
    13081 aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt
    13141 ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc
    13201 agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc
    13261 aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt
    13321 tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca
    13381 atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg
    13441 acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca
    13501 tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag
    13561 gtgcttttta cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt
    13621 tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt
    13681 taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg
    13741 cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg
    13801 tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa
    13861 aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg
    13921 gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca
    13981 ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg
    14041 gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt
    14101 caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt
    14161 taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg
    14221 cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa
    14281 ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt
    14341 cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg
    14401 cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga
    14461 tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt
    14521 gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt
    14581 agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg
    14641 tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca
    14701 agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg
    14761 attaatctta ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt
    14821 tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca
    14881 ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat
    14941 tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg
    15001 tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa
    15061 cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta
    15121 tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat
    15181 aaattattct cattataaag gagttcataa acttatgatt atatttgaca atcacacaca
    15241 aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta
    15301 tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc
    15361 ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt tattatcttt
    15421 agacacatta ccattagaat ttccaggata tggacatggt gattttagag aaccggcgca
    15481 taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata gctacgaaat
    15541 tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag atgatgatga
    15601 agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt tacagttgtt
    15661 ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag taaataatag
    15721 tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc caaatcgtcc
    15781 actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa
    15841 aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc atcaacaaaa
    15901 tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg catatggatt
    15961 taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat ttgaccaaac
    16021 acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa ctggcgactc
    16081 atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata cgttatcaca
    16141 gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt taaaagaacg
    16201 tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag aaaaaatcat
    16261 gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag atgatggttg
    16321 gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa cagaaggcaa
    16381 acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga tgaaatttgg
    16441 gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag cacatccaga
    16501 ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga
    16561 tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga tttttgatcg
    16621 tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag tctattctgt
    16681 cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag ggttatatga
    16741 atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt gttcgggtgg
    16801 aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga cgagcgacaa
    16861 taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt atccaatttc
    16921 aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag aaacgagttt
    16981 aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag atctaacgca
    17041 attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata aaaagcatcg
    17101 ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag gcaatgatac
    17161 tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt tccgagtatt
    17221 agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag aagaagcaac
    17281 ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg gtttttatat
    17341 tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac agatatagta
    17401 gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg aagaaaatat
    17461 gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa atgaagaagt
    17521 acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag gcggatttag
    17581 aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg aatctgatgg
    17641 agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca ttgaagtgtg
    17701 gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg tagcagaagc
    17761 cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt attcgatggt
    17821 ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt ttaaagatag
    17881 ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag ttacgcaaga
    17941 taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa ttaataaata
    18001 tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc tttttatgtt
    18061 actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca acatagaaat
    18121 ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac gggcattaga
    18181 atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc gggataattc
    18241 tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt ctgataatgt
    18301 atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt taaccaatca
    18361 acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata gtgtatacaa
    18421 agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac atattttgct
    18481 ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta cagcaacaga
    18541 acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt atgaagatgt
    18601 tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg tattagctaa
    18661 ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta tgcagatagt
    18721 ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca aggtgttatt
    18781 ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt tgcgccgagt
    18841 gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc attaggtgaa
    18901 gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc aaaatttttc
    18961 caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat ccgtattcat
    19021 gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa
    19081 gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat
    19141 acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt
    19201 cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt
    19261 gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa
    19321 cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc
    19381 catgagcatt attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc
    19441 gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat
    19501 tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt
    19561 gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg
    19621 tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat
    19681 atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg
    19741 ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta
    19801 ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat
    19861 cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt gattcaacaa
    19921 ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt
    19981 gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt
    20041 gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga gtctaaagat
    20101 ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc
    20161 tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat acatggataa
    20221 gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag gaaaactttc
    20281 tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac tgtcttttga
    20341 agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt
    20401 gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat cgattagtca
    20461 tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag aaatttttca
    20521 acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc atctgtatct
    20581 gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga ctttccgtca
    20641 aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata ctacgatgta
    20701 tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac taccagtatt
    20761 ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact ttgaaaagat
    20821 ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa acaccaaagt
    20881 tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag acgagctggt
    20941 tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg ttcaagataa
    21001 gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc
    21061 tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca aacaacacat
    21121 gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac
    21181 gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg aaaaatcagt
    21241 gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat gttgtctaga
    21301 aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt tttcagttga
    21361 ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag ttttttttga
    21421 cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca acactagtga
    21481 tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc aagtatacaa
    21541 agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact atttttagta
    21601 gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta taacaaaaaa
    21661 tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac gacaggcaaa
    21721 agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag gactagtacc
    21781 tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg attttcagaa
    21841 tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct agtttgggat
    21901 tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa cagttaaaga
    21961 agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga aacttctcaa
    22021 agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta tcaagggaaa
    22081 gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt tcttatttct
    22141 ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa aataatttat
    22201 tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag atgtcattag
    22261 atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt ggaaatgtga
    22321 agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa gttatttcaa
    22381 ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg ttacttttta
    22441 tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt gattttgaaa
    22501 ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc tatgagtttg
    22561 tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat acaattacct
    22621 ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt gtactttctt
    22681 ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga catgcaacga
    22741 tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct tctttgctta
    22801 tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa
    22861 cgactttagg aacttatggt catttgtatc taaattcatt gagagagata acaaatgatc
    22921 ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt tacgataata
    22981 aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag aaaaactata
    23041 atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt atactcataa
    23101 agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag ttgttgtatt
    23161 ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct gttggtagag
    23221 tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa agccttttga
    23281 atattttgct accatccgtt cgattgcaat accggatata cgcttggctt ctccttggta
    23341 aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt tccgaatagc
    23401 tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat ctcttttatt
    23461 tccttttcta gttactacca cattattctt atctaaagat aggtcatcta aattaatatt
    23521 tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg ccaggtctct
    23581 ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt tatattcatt
    23641 ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt tctcaatggc
    23701 attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat ccataggatt
    23761 attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat atttaaatag
    23821 aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta agttattatt
    23881 atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa
    23941 atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg tgatagaagt
    24001 atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat tttttagata
    24061 ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa aatcttttat
    24121 ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg tatttttcat
    24181 gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga ctataccaat
    24241 tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc aaaaaaagca
    24301 tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata gggaaggaaa
    24361 tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga aagaacaatc
    24421 tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca agcgtatata
    24481 ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag aaaatttagg
    24541 gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga aaatgggtta
    24601 aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa
    24661 caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga atatgatact
    24721 acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa gatattataa
    24781 aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt gtaaaggatg
    24841 cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt gaagaggttt
    24901 atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag aaacatcctt
    24961 ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg aaaatcaatg
    25021 gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat attgctacgt
    25081 atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag acacatacgc
    25141 agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct atagcgaaat
    25201 aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa acgtaaattt
    25261 tctatatagt tggattaaga taacgactat ttattttatc atatttttac tataccctat
    25321 acttttactt ggtatattta tgaataatcg caaatttacc cctatactac tagtttgtaa
    25381 gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc tacaacttat
    25441 aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag taaagtttgt
    25501 tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc tgaaaactta
    25561 tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac taagcaataa
    25621 aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat tctgataaat
    25681 gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga tctcttcagt
    25741 atcagtaata ggataggcga taggccacat accctttgat ggttagaata aactgctcaa
    25801 aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa ttataaacca
    25861 taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca gaagatataa
    25921 ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta aagggaattg
    25981 tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc ccctgctagt
    26041 ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga agtgagtcat
    26101 ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc tacggcattc
    26161 taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga acttatgtag
    26221 tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct tagcagtaaa
    26281 cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa gtcccaaatg
    26341 gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt ttggttgcct
    26401 cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt gaatcaatca
    26461 tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc aatggtagat
    26521 gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat ttaaattgat
    26581 cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt agaattaaat
    26641 tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact tcctcaactc
    26701 ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta gtataagtcc
    26761 tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata ttacctccat
    26821 aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt ttaatccgtt
    26881 ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga tttataaaat
    26941 atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa aatacgaata
    27001 tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta aatatcaaaa
    27061 tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta agaactgtta
    27121 agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga aagtggtaaa
    27181 actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg cgttgggtaa
    27241 aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc agatgaaaca
    27301 gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc gattgccata
    27361 ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc atccttttta
    27421 ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga tgttttcacc
    27481 agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga aaattcaccg
    27541 cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt ccaaaattaa
    27601 gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt ctttttaaac
    27661 aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt ttatgacttt
    27721 cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg gacgtttacg
    27781 agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg aactaccccc
    27841 acttagc
//
LOCUS       DAZKOM010000024        27490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000024 DAZKOM010000000
VERSION     DAZKOM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.024"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG8NF_001064"
     CDS             275..1753
                     /locus_tag="ACG8NF_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471555.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="ACG8NF_001065"
     CDS             2030..4090
                     /locus_tag="ACG8NF_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0471556.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="ACG8NF_001066"
     CDS             4173..4742
                     /locus_tag="ACG8NF_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0471557.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="ACG8NF_001067"
     CDS             complement(4917..5723)
                     /locus_tag="ACG8NF_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471558.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="ACG8NF_001068"
     CDS             5861..7375
                     /locus_tag="ACG8NF_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0471559.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7778..8761)
                     /locus_tag="ACG8NF_001069"
     CDS             complement(7778..8761)
                     /locus_tag="ACG8NF_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0471560.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8766..9413)
                     /locus_tag="ACG8NF_001070"
     CDS             complement(8766..9413)
                     /locus_tag="ACG8NF_001070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0471561.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9498..10130)
                     /locus_tag="ACG8NF_001071"
     CDS             complement(9498..10130)
                     /locus_tag="ACG8NF_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0471562.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACG8NF_001072"
     CDS             complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACG8NF_001072"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0471563.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10694..11431)
                     /locus_tag="ACG8NF_001073"
     CDS             complement(10694..11431)
                     /locus_tag="ACG8NF_001073"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0471564.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11428..12417)
                     /locus_tag="ACG8NF_001074"
     CDS             complement(11428..12417)
                     /locus_tag="ACG8NF_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0471565.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12675..13217
                     /locus_tag="ACG8NF_001075"
     CDS             12675..13217
                     /locus_tag="ACG8NF_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0471566.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13234..14319
                     /locus_tag="ACG8NF_001076"
     CDS             13234..14319
                     /locus_tag="ACG8NF_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471567.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14331..15137
                     /locus_tag="ACG8NF_001077"
     CDS             14331..15137
                     /locus_tag="ACG8NF_001077"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471568.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15138..15965
                     /locus_tag="ACG8NF_001078"
     CDS             15138..15965
                     /locus_tag="ACG8NF_001078"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471569.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15962..17035
                     /locus_tag="ACG8NF_001079"
     CDS             15962..17035
                     /locus_tag="ACG8NF_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0471570.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17463..20159
                     /locus_tag="ACG8NF_001080"
     CDS             17463..20159
                     /locus_tag="ACG8NF_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0471571.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20224..20724
                     /locus_tag="ACG8NF_001081"
     CDS             20224..20724
                     /locus_tag="ACG8NF_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0471572.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            20736..21230
                     /locus_tag="ACG8NF_001082"
     CDS             20736..21230
                     /locus_tag="ACG8NF_001082"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0471573.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21251..22060
                     /locus_tag="ACG8NF_001083"
     CDS             21251..22060
                     /locus_tag="ACG8NF_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0471574.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22057..22878
                     /locus_tag="ACG8NF_001084"
     CDS             22057..22878
                     /locus_tag="ACG8NF_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0471575.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22929..25028
                     /locus_tag="ACG8NF_001085"
     CDS             22929..25028
                     /locus_tag="ACG8NF_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0471576.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25025..26143
                     /locus_tag="ACG8NF_001086"
     CDS             25025..26143
                     /locus_tag="ACG8NF_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0471577.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26182..27060
                     /locus_tag="ACG8NF_001087"
     CDS             26182..27060
                     /locus_tag="ACG8NF_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0471578.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27206..27403
                     /locus_tag="ACG8NF_001088"
     CDS             27206..27403
                     /locus_tag="ACG8NF_001088"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0471579.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8793 a         4712 c         5750 g         8235 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     7501 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     7561 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     7621 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     7681 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     7741 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     7801 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     7861 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     7921 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     7981 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     8041 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     8101 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     8161 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     8221 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     8281 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     8341 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     8401 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     8461 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     8521 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     8581 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     8641 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     8701 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     8761 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     8821 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     8881 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     8941 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     9001 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     9061 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     9121 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     9181 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     9241 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     9301 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     9361 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     9421 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     9481 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     9541 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     9601 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     9661 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     9721 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     9781 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     9841 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     9901 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     9961 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
    10021 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
    10081 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
    10141 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
    10201 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
    10261 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
    10321 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
    10381 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
    10441 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
    10501 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
    10561 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
    10621 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
    10681 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
    10741 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
    10801 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
    10861 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
    10921 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
    10981 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
    11041 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
    11101 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
    11161 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
    11221 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
    11281 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
    11341 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
    11401 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
    11461 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
    11521 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
    11581 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
    11641 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
    11701 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
    11761 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
    11821 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    11881 aaaggtaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    11941 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    12001 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    12061 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    12121 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    12181 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    12241 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    12301 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    12361 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    12421 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    12481 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    12541 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    12601 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    12661 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    12721 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    12781 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    12841 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    12901 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    12961 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    13021 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    13081 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    13141 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    13201 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    13261 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    13321 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    13381 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    13441 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    13501 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    13561 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    13621 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    13681 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    13741 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    13801 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    13861 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    13921 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    13981 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    14041 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    14101 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    14161 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    14221 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    14281 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    14341 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    14401 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    14461 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    14521 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    14581 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    14641 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    14701 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    14761 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    14821 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    14881 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    14941 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    15001 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    15061 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    15121 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    15181 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    15241 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    15301 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    15361 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    15421 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    15481 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    15541 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    15601 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    15661 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    15721 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    15781 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    15841 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    15901 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    15961 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    16021 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    16081 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    16141 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    16201 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    16261 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    16321 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    16381 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    16441 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    16501 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    16561 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    16621 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    16681 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    16741 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    16801 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    16861 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    16921 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    16981 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    17041 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    17101 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    17161 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    17221 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    17281 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    17341 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    17401 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    17461 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    17521 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    17581 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    17641 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    17701 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    17761 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    17821 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    17881 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    17941 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    18001 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    18061 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    18121 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    18181 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    18241 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    18301 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    18361 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    18421 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    18481 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    18541 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    18601 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    18661 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    18721 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    18781 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    18841 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    18901 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    18961 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    19021 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    19081 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    19141 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    19201 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    19261 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    19321 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    19381 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    19441 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    19501 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    19561 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    19621 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    19681 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    19741 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    19801 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    19861 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    19921 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    19981 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    20041 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    20101 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    20161 gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt
    20221 aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac
    20281 agaaagaaag gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct
    20341 tcaggctttc ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt
    20401 aatgcttatg tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg
    20461 aacgagcagg atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag
    20521 attaccaaaa cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg
    20581 ccaattctgc tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa
    20641 ctggtaaata aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta
    20701 aatgaagagg aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg
    20761 aacgattaat tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt
    20821 tagtagtagc aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg
    20881 ctgcaccatc gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa
    20941 aagatccgcg ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg
    21001 taaaagttgc gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga
    21061 aaatgacacc gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt
    21121 cagaaattca agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta
    21181 caccggcgat ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag
    21241 gagaaaaatt atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg
    21301 acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg
    21361 agcgttgatg ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt
    21421 tatgggtgtc gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct
    21481 ggctacagcg ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt
    21541 tccactagca gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc
    21601 gatggctgct aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct
    21661 gcacttcttt atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt
    21721 tttatttgga gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa
    21781 cggattaaca gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat
    21841 gctttggagt aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct
    21901 cgagttacca ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca
    21961 aagagatcta caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa
    22021 tgcaacgctt gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct
    22081 aaagatgaga aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg
    22141 gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat
    22201 catttctaca aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt
    22261 tcgacgacca ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa
    22321 aattctgaaa aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg
    22381 ggaccattgg ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg
    22441 ggactagcga tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt
    22501 atgttcaata tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta
    22561 ggctctaaat atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct
    22621 gctagtatct tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat
    22681 accgttttga gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat
    22741 agtattttta aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat
    22801 aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca
    22861 ttacttggaa tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga
    22921 gggaaaagat gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg
    22981 agttaaacga cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg
    23041 atgtaaaggg attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt
    23101 gcgggacgat tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag
    23161 gtgtttgggt caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa
    23221 acgggattgt ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag
    23281 actgtttgcc tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg
    23341 ctcccatact ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg
    23401 aaacacacga aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt
    23461 cggatcagca aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg
    23521 aagtggcaag cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt
    23581 ttcaggaatc tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat
    23641 ttgggcagct gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc
    23701 aaagcttttc tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca
    23761 gctatcctgc tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca
    23821 ccatgtttga accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta
    23881 agtttcctgg ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca
    23941 gtgcgttact ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt
    24001 gtccgcaggt tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt
    24061 caggggaacc aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt
    24121 atcataccgg cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg
    24181 aagccctaga acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg
    24241 aatcaatttg taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa
    24301 tcggtgatga ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc
    24361 aagggttaat ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg
    24421 atggagaaga atcagacact acgttgatgc ctttttatca atttttagat aataaagatc
    24481 atttatatca gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg
    24541 aaatcaaagg catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg
    24601 ttttaatgag cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt
    24661 gtttggcaga tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag
    24721 gagaagtcgt gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca
    24781 gcctaatgat cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc
    24841 ctgttgataa tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga
    24901 ttgaatgtac gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa
    24961 gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa
    25021 aaagatgagt atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac
    25081 tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc
    25141 aagccgcaaa ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga
    25201 tattactgga acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt
    25261 tggcagtttt gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc
    25321 tgctgttgaa agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt
    25381 gaactcgttg ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc
    25441 tttccaaaca tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt
    25501 tttttatacg ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca
    25561 cgcaaattgg catcgggaac gcattaccaa attacaggag gattataagt taattgatcg
    25621 attaaagggt caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta
    25681 ttggtggggc gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt
    25741 aacctcaaca ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga
    25801 gttcggacgt ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc
    25861 aaatagagat gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt
    25921 tggtgacgac gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg
    25981 ttttaccgaa gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt
    26041 tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa
    26101 tactaaacgt catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat
    26161 attcgaaggg aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt
    26221 gtctgtgcag tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca
    26281 actccagttg aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg
    26341 gcaggcatcg gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc
    26401 ggtcatatca catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta
    26461 aagcaatatt tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt
    26521 gaatatgttg ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca
    26581 ggtatcggag gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa
    26641 atggggcata tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc
    26701 caccaagatt gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta
    26761 aaaggccaga atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc
    26821 caatgtgcat acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt
    26881 gtaatgaaac aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat
    26941 ggatatgttg aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat
    27001 gccggaataa tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa
    27061 ttaatgaatg tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca
    27121 aatgaagttg gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt
    27181 gttttcatta aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc
    27241 gctgtatacc ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta
    27301 ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt
    27361 tgacttcata atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt
    27421 ataaaataaa tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa
    27481 aaaagaaagt
//
LOCUS       DAZKOM010000025        25902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000025 DAZKOM010000000
VERSION     DAZKOM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.025"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..647)
                     /locus_tag="ACG8NF_001089"
     CDS             complement(195..647)
                     /locus_tag="ACG8NF_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0471580.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(660..1070)
                     /locus_tag="ACG8NF_001090"
     CDS             complement(660..1070)
                     /locus_tag="ACG8NF_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0471581.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1345..1557
                     /locus_tag="ACG8NF_001091"
     CDS             1345..1557
                     /locus_tag="ACG8NF_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0471582.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1560..1772
                     /locus_tag="ACG8NF_001092"
     CDS             1560..1772
                     /locus_tag="ACG8NF_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471583.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1912..2067
                     /locus_tag="ACG8NF_001093"
     CDS             1912..2067
                     /locus_tag="ACG8NF_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471584.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2064..2288)
                     /locus_tag="ACG8NF_001094"
     CDS             complement(2064..2288)
                     /locus_tag="ACG8NF_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0471585.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2366..2554
                     /locus_tag="ACG8NF_001095"
     CDS             2366..2554
                     /locus_tag="ACG8NF_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471586.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2551..2724
                     /locus_tag="ACG8NF_001096"
     CDS             2551..2724
                     /locus_tag="ACG8NF_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471587.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2693..2821
                     /locus_tag="ACG8NF_001097"
     CDS             2693..2821
                     /locus_tag="ACG8NF_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471588.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2880..3215
                     /locus_tag="ACG8NF_001098"
     CDS             2880..3215
                     /locus_tag="ACG8NF_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471589.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3448..4389
                     /locus_tag="ACG8NF_001099"
     CDS             3448..4389
                     /locus_tag="ACG8NF_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0471590.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4391..5281
                     /locus_tag="ACG8NF_001100"
     CDS             4391..5281
                     /locus_tag="ACG8NF_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0471591.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5324..6124
                     /locus_tag="ACG8NF_001101"
     CDS             5324..6124
                     /locus_tag="ACG8NF_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0471592.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6139..6990
                     /locus_tag="ACG8NF_001102"
     CDS             6139..6990
                     /locus_tag="ACG8NF_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0471593.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6987..7190
                     /locus_tag="ACG8NF_001103"
     CDS             6987..7190
                     /locus_tag="ACG8NF_001103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471594.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIIY
                     RDTERFLTVLGNVYENPELLEVE"
     gene            7191..7652
                     /locus_tag="ACG8NF_001104"
     CDS             7191..7652
                     /locus_tag="ACG8NF_001104"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0471595.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7678..8130
                     /locus_tag="ACG8NF_001105"
     CDS             7678..8130
                     /locus_tag="ACG8NF_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471596.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8236..8430
                     /locus_tag="ACG8NF_001106"
     CDS             8236..8430
                     /locus_tag="ACG8NF_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471597.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8461..8754
                     /locus_tag="ACG8NF_001107"
     CDS             8461..8754
                     /locus_tag="ACG8NF_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471598.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8685..9224
                     /locus_tag="ACG8NF_001108"
     CDS             8685..9224
                     /locus_tag="ACG8NF_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0471599.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9299..9766
                     /locus_tag="ACG8NF_001109"
     CDS             9299..9766
                     /locus_tag="ACG8NF_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0471600.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10513..10743
                     /locus_tag="ACG8NF_001110"
     CDS             10513..10743
                     /locus_tag="ACG8NF_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471601.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10902..11735
                     /gene="terS"
                     /locus_tag="ACG8NF_001111"
     CDS             10902..11735
                     /gene="terS"
                     /locus_tag="ACG8NF_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0471602.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11728..13143
                     /locus_tag="ACG8NF_001112"
     CDS             11728..13143
                     /locus_tag="ACG8NF_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0471603.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13155..14684
                     /locus_tag="ACG8NF_001113"
     CDS             13155..14684
                     /locus_tag="ACG8NF_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0471604.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14602..15654
                     /locus_tag="ACG8NF_001114"
     CDS             14602..15654
                     /locus_tag="ACG8NF_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0471605.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15656..15973
                     /locus_tag="ACG8NF_001115"
     CDS             15656..15973
                     /locus_tag="ACG8NF_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471606.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16100..16723
                     /locus_tag="ACG8NF_001116"
     CDS             16100..16723
                     /locus_tag="ACG8NF_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0471607.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16738..17628
                     /locus_tag="ACG8NF_001117"
     CDS             16738..17628
                     /locus_tag="ACG8NF_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0471608.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17651..17866
                     /locus_tag="ACG8NF_001118"
     CDS             17651..17866
                     /locus_tag="ACG8NF_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471609.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17878..18219
                     /locus_tag="ACG8NF_001119"
     CDS             17878..18219
                     /locus_tag="ACG8NF_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471610.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18219..18590
                     /locus_tag="ACG8NF_001120"
     CDS             18219..18590
                     /locus_tag="ACG8NF_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471611.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18583..18978
                     /locus_tag="ACG8NF_001121"
     CDS             18583..18978
                     /locus_tag="ACG8NF_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0471612.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18980..19357
                     /locus_tag="ACG8NF_001122"
     CDS             18980..19357
                     /locus_tag="ACG8NF_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0471613.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19358..19966
                     /locus_tag="ACG8NF_001123"
     CDS             19358..19966
                     /locus_tag="ACG8NF_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0471614.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19966..20307
                     /locus_tag="ACG8NF_001124"
     CDS             19966..20307
                     /locus_tag="ACG8NF_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471615.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20549..23338
                     /locus_tag="ACG8NF_001125"
     CDS             20549..23338
                     /locus_tag="ACG8NF_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0471616.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23328..24068
                     /locus_tag="ACG8NF_001126"
     CDS             23328..24068
                     /locus_tag="ACG8NF_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0471617.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24065..>25902
                     /locus_tag="ACG8NF_001127"
     CDS             24065..>25902
                     /locus_tag="ACG8NF_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0471618.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9352 a         3713 c         5269 g         7568 t
ORIGIN      
        1 tcattttttt gttctaattc ataagcttct ttttttattg tgttagcttt cgacagttta
       61 gtgattccta taattaataa aataagtcca atcaataata acattcaaaa ctcctcattt
      121 ctttgatata attattgtag agtttactca gaaatgagga aagagtccat gtgaaagcat
      181 gggctttttt atttttaatg ttgtaatact aaatgttcaa gattatgagg aagccctaaa
      241 tattcaataa tttggaactg actcatatca gataaatctt gttcatctat tgttctagtt
      301 aacatttcaa ttgcaaacat attagcttca gcttctatac cataaatcat acaactaagg
      361 ttgtttgctc taaaagctgc tgtgcaaata cctttatgga gtctaacgtg ggctatttca
      421 tgccataata caaatctttt catagaaggt gataggtttt gattaattaa tatcacataa
      481 catcttttat tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt ttcaattaaa
      541 tctatatcag aatatttgat tattttatat ggattaatcg catcagatcc aaactctaca
      601 aataattttt ctatttcttc taatatccag tatttgttta aatgcatacc aaagcccacc
      661 tattgacgat attttttagg agtgaatttt ttctttgctt taactttggt taaggcaagt
      721 gcaagttgaa tggcatcgta taatttttcc ttatcttcat cgtctaaagg ttcaccataa
      781 taatttgttt cagcatctga attcaagcct tcaagtaatt tttcagcttg taaagcaata
      841 tcttgtttat ctttttctgt taaatcataa taatggcgtt tatcagtacg tcctaaaaga
      901 taatcagttg atacattaaa gtaatctgca actttttgta ttttatctcc agatggagta
      961 cttttattcc atctagacat gcttccattt ccgaaattta actttctttc aagttctgcc
     1021 aacgtcattg aacgttcttt taataagatt tttatacggt ctaatagcat aggatatgca
     1081 ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa attatctatt tacaaataga
     1141 gaatttgcag ttatactagt tttgtaagca agatatttac ggcatacaaa gactaaaagc
     1201 aataaactaa tagtctcccc agacatcagt aagctcgctt tttgtgcgct gtattttgta
     1261 tgcttacata atagcatatt tgcagttttt gtcaaactta ttagacaaaa atattagaaa
     1321 atattcgata agggggtatg aagcatgact ttaaaacagc aagtattaat tgctttagta
     1381 aagaaaggtt ggagtcaaag agagttagct cgacgaatgg gtatatcaat tacttattta
     1441 cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat cgaagaactt
     1501 ttggatatca agttagaagt agattctaat gatcagccat cagaacagga ggcgtaaaaa
     1561 tggtacaacg tttgagcaaa gcggagttta aagagaaata taagtattct gaaagtactt
     1621 atcaaagaag aatgaaggaa tttaaaaaat cacgttttag tgaggggtat aacgcagtta
     1681 cttccaaaga aataacaatt gatgtcgatt tatatgaaca gtttagaaca tggcgttcta
     1741 aaaatcgatt tagagttaga gctgtttgtt aatagctaaa aaatctaaat tcaccacttc
     1801 tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa gtccacgttt tttcattttg
     1861 gcaactcctc ttcttggagg tggtgacggc gagagacgaa ggagggatga catgaaggaa
     1921 gaatcaaaaa tagggatttt gaatgagaac aattacacaa gattaattat tgaaacagaa
     1981 gaagggaaaa aagtagcaga agttacattg accgatgcaa ctcctgctga cggttatcgt
     2041 attcgattaa ctcctaaata tgattaacct tttggtgggt gtggatcgtt tccgtgacta
     2101 tcacggcttt gaatttttcc atctctgcca tgaataaata gctcagatcc ttgattttta
     2161 gaaatctctc tagcgatttt tactgcttct gattttgtat tagtatgtgc agtcgcttta
     2221 gaatttccag cacctttgac attccaacca ccattggaat taggtacaac atgttgattt
     2281 tttcccatag ttttcacctc catttctata gaaatatttt atcaaataga aatggagaaa
     2341 ttcaataaac gaaaggagaa gtgatatggc atataccttg caacaagaac atcaaattct
     2401 cggtttgatt aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc ttagaaaagc
     2461 cgatgagcta tcagataggc aagctgaact aattgcttct gaacttgagg atttgagaat
     2521 gctagaaata aaaaataggg agattagatt atgaagaaga cagacacact ttttatagga
     2581 ttcattttgg ggttattagt gattgtagcg caccaaagta ttatcggggg aagcttgttc
     2641 gcagcattga tggttttaat caatctgctt gattcaaaag aaaggagcaa ctatggcacg
     2701 agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa atagccgccc
     2761 tattatttta agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg
     2821 actctgccag ccagcaaaga gtcacataca aatttataaa actaggagaa ttatatcaca
     2881 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
     2941 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
     3001 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
     3061 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
     3121 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
     3181 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
     3241 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
     3301 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
     3361 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
     3421 aagcaccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
     3481 gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac aattttagga
     3541 ttgaataaat ggaaatctcc ttatcaacta tggctagaaa aaacaggaca agtagtactt
     3601 gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt ggttgctaaa
     3661 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
     3721 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
     3781 gaatgcaaaa cagccaatca atttctcggt aaagaatggg aaggcgaaga ggtaccgctt
     3841 agctatctct gtcaagttca acattacatg aacgttctaa acaaagacta ttgttacatc
     3901 gctgtcttaa tcggtggtca aaaatttatc tggaagcgga ttgaacgaga tcaagagctg
     3961 atcgatacaa tcactgaaca attagtagag ttttgggaaa caaacgttct tggaggtatc
     4021 gagccaatta ttgatggtag tcaagcgaca gctgatttct tgaaagagaa gtatgcagat
     4081 gttgaagacg ttcaaacgac gttaccaatt catttcgatg aactagttga acagaaaaac
     4141 gaactcaaac ggactaagaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt
     4201 atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt
     4261 tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat
     4321 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaagggag
     4381 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa agccacagaa
     4441 acaagttgca ccaggtcaat taggacttaa agctttgatg aatacaccaa caatgagaaa
     4501 gaagttcgaa gaggtgcttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
     4561 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
     4621 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
     4681 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
     4741 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
     4801 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
     4861 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
     4921 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
     4981 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
     5041 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
     5101 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
     5161 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
     5221 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
     5281 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
     5341 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
     5401 atggaacttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
     5461 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
     5521 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
     5581 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
     5641 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
     5701 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
     5761 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
     5821 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
     5881 tctgcaaagt tgggcagatg acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
     5941 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
     6001 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
     6061 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
     6121 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
     6181 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
     6241 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
     6301 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
     6361 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
     6421 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
     6481 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
     6541 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
     6601 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
     6661 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
     6721 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
     6781 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
     6841 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
     6901 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
     6961 ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc
     7021 gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga
     7081 gtcgaatggt tcggaagata agtaagctaa ttatttatcg ggatacagaa agatttctca
     7141 ctgttttagg taacgtctac gagaatccag agttattgga ggtagagtaa ttgccaaaaa
     7201 tattagatgc atgttgcggt agtagattgt tctggtttga taagaacaat tctgacgtaa
     7261 catttatgga ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg atcaatgttg
     7321 atccagatgt ggtcgcagac tttcgcgata tgccttttga agatgatgtg ttcgatatgg
     7381 ttgtatttga cccgccacac ttaagacatg tcggcgaaaa ctcatggctt gccaaaaaat
     7441 atggaaaatt agacgaactt tggccagaag acatcaggca aggttttgca gagtgtatgc
     7501 gagttttacg accttcagga acattaattt ttaagtggaa cgaggaacag ataccacttt
     7561 ctgatgtgtt agaggctatt ggagaacagc cattgtttgg taataaacga agcaaaacac
     7621 attggcttgt gtttatgaaa aaattcagct aacggaggaa acaaaggagg aaaagtaatg
     7681 aagattgtat taaataaatg ttttggtggc tttgggttga gccatgaagc aaaaatggaa
     7741 atttttaaaa gaaaaaatat tgaagtattc ccatacatca ataactttag ctatgactca
     7801 gatgatgaat acacgagata cacaggccaa aagctaggtt cgatggattt tatctactat
     7861 ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt atagcgaaat tgaccgactg
     7921 tacggaattg ctgatgattc cagtttttca agtgattcaa acagaggtga taaggacctc
     7981 gttgctgttg tcgaaaaact aggaagtgag gccagtggac catatgcaag tcttaaagtc
     8041 gttgatatac ccgatggggc tgagtgggaa ataagtgatt atgacggcgt tgaaactgca
     8101 cattatggtt ttcaaacagg tagtatttag tccactaatg acaactaaag aggaggcaga
     8161 aggatgagtg aagatgaata ctttgaacag tagtctatta acgacagaga tttttaactg
     8221 gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata aaattaatag tctcaaagag
     8281 gttttcggag acgtcgaagt agtgataaac gcacaaggct atgacaacga attttttaag
     8341 cagattaccg acattagcat tcagcaagga atggaagacg aagacggaaa ctttatcgat
     8401 gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg aggaagcgga
     8461 atgaaactaa aagacggatt ttactccagc ggccacggca tcggcggttt aatgctagat
     8521 atgccgacaa agaatcctaa aacacgtaag aaaccaaaat tcaaagtcgg tgacatggtt
     8581 cgctgcgaag cagaaggatt catctatcca tttcgtggat atgtagaaca cgtctataat
     8641 cactcagcaa tcattcgcat tgaaaacacg atggaatgtg ataagtggtt agcgaaaagc
     8701 aaagagaatt tagcagtggt gagattggtg gacatggaag ttataaaatc ttaattttgc
     8761 ttttttacaa ctatcctaca acaatgagcg ttattccaca actggaagga gaagaggcaa
     8821 ttgtgaacat tttagagaat atagatatta agcaaacaag gaaaaatgct agacgattac
     8881 taaaaagata cagaaattta gaacgtttgg tcggaccagt gaaaatagac ttttccgtga
     8941 tgactgttac caaaaatttg aaattcacaa ttgacagtca aaacgaagaa atcattgaag
     9001 cgataagtac tcgagattcg gttatcgaag cattaacacg gctaagtaga atccatttcc
     9061 aggtacttta ttatagttat tgttttccta ataagatgtc aatgtatcaa ataggagaaa
     9121 aattaggata ttccgataga actatcgaga gaatgaaggc agtagctttg gttgaatttg
     9181 ctgaggcgta taaatcagga gaactcattt cacgtacaaa ataaaaaaag tcggattcct
     9241 ccgactatga ataatatttc cgacataagt attatatcat aattggggga atcagaggat
     9301 ggtacttttc gatgtaaaga aatatgagac accaagcgcg aaagatgttg atatggaacg
     9361 cacaaaacat aatgtcgctg tgtttctttc agcatatcta tcagctagat gtagagtagg
     9421 gcaacatcga gaaccaaaag tgacagcatc ttattccttg gttccacctt ctacagctga
     9481 tcatatattt gaagcagaaa gaatgctgat cgataaagaa gaagcacagg aagaatttga
     9541 gtatctgcat aaattgtttg ttcgaggcta ttcagcgata cagcatccgc ataagcctga
     9601 tgtgactgaa aggcgcaaga agatattcta tgatcgttat atcaatggtc tgcccattta
     9661 tgtaactgct caaaggaata atactagcga agaatcggtt aaagtagaat caaacagaat
     9721 tatcatccaa tttgcttcat cgttagaact ggttgctttc aagtagccag tttttacact
     9781 ttttataccc ttttattacc aatttggttt ccattttata ccttttttat accaatcact
     9841 tacctattca acgttgtatt atgatagtgt cgaaaaatta ggaaacagga tcgacaaaat
     9901 aaaatgtaag ggaggaaatc tccctcatcg tttaattaag cttcgatagg cagcaacgga
     9961 aatattaaga ataaggatgt gaatttcaac tccttctaaa ttgttcttat tatctatcat
    10021 ccgttgctgt ctattgtttt tttaattatt cacacgataa ctaaaggtaa gtgaagagaa
    10081 atgattccat taataatttc aatttttgcg ctttgtctta atgtatatat gattggattt
    10141 aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg tcactgtggc
    10201 ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca tgcaaggttc
    10261 gattccttgc cagtgacttt ggcaaccgag gcatcggcgg tttaaaaata taggggtgcg
    10321 caatttcgta cgcgttttgt gcatcgtgta ggttgctatt acataactgg ttaggttaga
    10381 ttgagatttg ggattcggta caaatgaatc gtcaaatgtc tcaagcacag gatcggaaac
    10441 gtccctgcct gtgcattaca tattagatca ctctttgagt ggtctttttc tttacataaa
    10501 ggaggctaca taatgagaaa ctactggtat gtatcgctaa ctaatgaata tcctcgaacc
    10561 attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt
    10621 gtcgaaatgg ggagagaagc agaaccatgt gagattgatg cgtgcaagct cgtttattgc
    10681 ggtcatggtt tctatgatga accaaacatt caaaataata ttaacaagaa tttgagggat
    10741 tagaatgcaa gaaatggctt aaaaatctcc aatcaggaca aatcaagtta gacaaagttt
    10801 ccgatttaaa gatattgatt gaagctgatt taatgctaaa agatattgaa aattagaaaa
    10861 caaaactcaa cctaaacaga atgtgaggtg gtgtgtattg aatggcaaga caacgtgatc
    10921 caagacgtga tgaagccaaa agaatttggt tagaatccaa cggagaaaag cagttaaagg
    10981 aaattgcatc tgaattaaat gtttcagatt ctcaggttag aaaatggaaa tcgcaagaca
    11041 aatggagcgc tgaattgatt agtaacgtta ccaatggcaa aagtaacgtt actaatcaag
    11101 gtggcgctcc tattggtaat caaaatgcta aaggtaacaa aggaaatagc cgagcttctc
    11161 cgccagtggg taataaaaac gctttgaaaa caggtgagta tgaaacaata ttttttgaca
    11221 cgttaaccga taatgagaag gacatctatt ctagtttgaa tgatgatcct tcttttgctt
    11281 tgtccgaaga aatacgactt cttaaaataa gacaacttcg aatgatgaaa agaatccaac
    11341 aagctgaagc tggactaaat gatgaagaag tcgaacgatt gcagcagcta agaaagatta
    11401 aaactccgat tgaaaaaaat ggtaaaaagc tagaaatcaa gcgtgaggtt atgcaagatg
    11461 tgcagattag cagaaaaaaa catcgcaaaa ttgatgatat tctttcaatt gaagattcat
    11521 tgactcggat tagcaaccag ttagctaaag ccatcaagca aatgaatgaa ctttatatga
    11581 atgaatacag aactgattta attaaagctc agactgataa gatccaagct gagacaaatg
    11641 aaattggcgg aaataattca ggtgaagaaa tagaagaatg gaaacaggca gttttaaatg
    11701 ccgcaaacaa acgggcggtg aaagaaaatg aataatgaat ttattccttt tgctgatatt
    11761 ggttctgcca ttgattatta ctatgataaa ccagtagctt tttgccaaga tattttgcat
    11821 ttgaatcctg atgaatggca agaaaatgtt ttaaatgatt tagctgaatt ttcaaaggtt
    11881 tctgttcgtt ctggtcaagg agttggaaaa acagcattag aagcaggagc aatactttgg
    11941 ttcttaacgt gtcgacccta cgctaaagta atagcaacag ctccgacaat gaagcaactt
    12001 tacgatgtac tttgggcaga ggtagctaaa tggttaaatg atagcttgat caaaaactta
    12061 ctgaagtgga caaagaccaa aatttatatg gttggtgatt cagagcgttg gtttgctacg
    12121 gctagaacag cgactaaacc agaaagtatg caaggttttc acgaggacca tatgttgatt
    12181 gtggtagatg aagcttctgg tgtgtctgat ccaattatgg aagctattct tggtacgcta
    12241 tcaggttttg ataataagct gttgatgtgt ggaaacccca ataatattga aggtgtcttt
    12301 tatgattccc ataattcaga ccgtgataaa tacagagttc ataaagtatc aagctatgat
    12361 agtaaacgta caaacaaaga caatatagaa atgattctta aaaaatatgg aaaagaaagt
    12421 gatgttgctc gtgtccgtat ttttggagaa tttccgaaag gtgcgttgga ttcatttatc
    12481 agtcttgaaa cagttgaatt ggctacagaa aaacaaatta gtgattcttt agtcaataaa
    12541 acaaaggttg ctcatattgg tgtcgacgta gctcgatatg gtgatgattc tacgattctc
    12601 tttcctagaa ttgctaccag ggcattggag tatgagaagt attcaaaacg tagcaccatg
    12661 gaaacaacag gatatgtcat caacatggcc aagaatctaa tgagtcaata tccgagtatt
    12721 gataaagtga tgattaaagt cgatgacact ggtgtcggag gtggtgtaac cgaccgccta
    12781 gaagaactta tagaagacaa acattatcct tttgaggtgt ttggagtgaa taacggttca
    12841 acatcagaag acgattttta cgataattta ggtactcaac tatggggaaa catcaaggaa
    12901 atgttagaag aaaatatgac agcaaatctt aacggagaac agcctgttat tgaattgcct
    12961 tctgatagtt cgttaatcaa agaattaagt actcgcaaat tcaaaatgac aagtagaagt
    13021 cgtatacgtt tagaaagtaa agatgatatg aaaaagcgaa atattggtag tcccgatatt
    13081 gctgacgcac tggctttagc gttttatgag ccaccaagtc actatcaatt tattcaattt
    13141 taggaggtga gcttttgatt agtacagtat tatctttaga gagatataag aagctacgtg
    13201 ttaaatatgc aacacaaatt gaagatggaa tgtttgatcc gaatggtttt atagaagata
    13261 tgaaaccatt ttttgctgat cgcgaaagaa aatatctagc ttatactagc gaaaagaatg
    13321 aaatagataa tagaccaaag cctaacactg atattgtaaa agttaataat aaacttcatg
    13381 ctggtatgta ttcaattgtc gtagaccaag cagtcaatca ttttactggt atacctatca
    13441 aatgggatta cgatgtatcg gaacaaaaaa gaacactcat tcaaagatta aaagataaat
    13501 tcttaaaaaa tgatatagaa cttccgacag ttccagaagc ttttaacaaa ttaacaagca
    13561 atcttgattc catgagattc gcaatgcttg attctgaaac tgcaactttt caaggagctt
    13621 gtggagtagc tttccgattg ctagagcctg tggaagaaga tgatggttgg aagttaagag
    13681 caagtaatat tgaaccttgg agagcagaaa gatatggaaa tgccggaatc tatatcaaag
    13741 aaaagtatga ctcttaccag aaaaaatttt ttcaagaaat gaaagttatg acaagaaata
    13801 aaattctgac atatgcttgc tatggtgatt tgaattttgt tacaagcgga acgttcaaaa
    13861 aaattgatga gaccgataac cctttgggga cgattacctt ggcagaattt aaaaataata
    13921 cgaatcgtta ttgtgatttt gaagtagctg aggaaattgg agatgcaatt gatcgtgctt
    13981 tgtctgatca acaaaacgaa atcgaacaat ttaagcttgc gtacatgctt gttactggca
    14041 caacaatgag caaaggcact gctaaagaaa tgatgaatca gctagggatt atcaacttaa
    14101 aagcccctac tgcaaaagct gaatatgtca caaaaaattt agcaaaagat ttcaatgaat
    14161 atcatatgga tctattgaaa aaacagtttt acaccatttg taaagcaatc gattttaacg
    14221 atgaggtgtt taaatcaaac agttctggag aagctcgcaa gtggcaaatc attagcctag
    14281 aagcgaaaac aaatactaaa gagcaatatt ttagagaagg attgaaagaa tgcgcagaaa
    14341 caattgctgc ttttcttaag tttcatgaca aagtagaaat tgagcctgaa aaaattattt
    14401 ttactttctc tagatctttg ccaactgatt taagttattt agctgaagct ttacctaaac
    14461 ttgcaccata cgtatccaaa cgaactattc agagccaaat tccatttgta acagatgttg
    14521 attatgaaaa tgaaatgatg gaattggaaa gtggaagtgc ttatccagat agtgaataca
    14581 attttggcgg aggtggcaat agtgacgata gaaacgaagt attggaccaa acgtcgagaa
    14641 ctggaggatc aagcaaggct caaacaagaa aatcagacac ttaaaaaatt aactagtgta
    14701 tttcctgaag cactgaaaga gatacaagca aaactattat cacaagctga cttacacaat
    14761 atcacttatc cagaaatgat ggagttttac agtacaagta atcagaaaaa atatcgtgaa
    14821 tatgtggaaa aaaattataa gtcattaaaa atgtatgatg caaaatacaa agagtttatc
    14881 gatgaatttt ttccaccatt tgactatgca aaagtcaatc gcttattaca aatacgatca
    14941 gatgtattta aaattcttgc agaatatgcg atggatgcag atgtgaatca atatttttct
    15001 gatcgcttag aggaaattct tcaaagaaca tattcttcta atgctaatgt ttttgttcaa
    15061 cttttaaacg ttgatatacc aaattattta ccagaaaatg aaattaaaca ttatttgaac
    15121 tatccatggt caggaaagac attttcaaga agactttggg gaaatatttc atcgcttgaa
    15181 cagaaactct ctaatgctat tgtaaaaagt gttgctagtg gagaaggcgt tatacacgca
    15241 ttaaacacca tgagattaga ctcagaaatt tgtgacatgt ttaagttaga agaatcaaag
    15301 tataacaaag cgatagaaaa tctcgttcga acggagtatg caaaatttgc acaagatggt
    15361 attgaaaaat catatttaga aacaggtatt gaggaataca acgtattgac tgcaaaagat
    15421 gagagagttt gccggatttg tggaggaaag gcaagtaaga atccctataa actgaaagat
    15481 gctgtcatag gtgaaaatcg agcacctttc catagtcgtt gtagatgtac ggatgttcct
    15541 aatttaccaa aattaggaaa ggatattgat gaagaatatg accgtttatt tggcgattta
    15601 ttagatgagt ttgcacatga ttcttttgga attaatttga aacggaggaa gtagaatgaa
    15661 agattttttt gaagcagtac taacaattaa tgtaaatgct gatattgcag aagcctacaa
    15721 aacagctatt gagtctgaga accatcctaa tggcttgaga gaccattgga atggcaatta
    15781 tgcctacgtg gttattggcg atcaaactgt taattatcaa gataatactc cagttgataa
    15841 gaataccgtt aatttaacga ttcaattatt atctcattca ttaccaaatt taaaagaaac
    15901 agttgattgg tatgaaaaga tgggatgtat tgttgttaga actgactaca aagaaggaaa
    15961 gtctagtaat taggcttttt tattttgtcc gaaatgacgt taaactagcg caatactggg
    16021 cttggttgaa tggtggggcg caactattaa aactcaaagc aatgcggggc gtgaaaacga
    16081 atcgtggggc gaaaggagaa tgagcatgaa acacaaatca ttaatgccaa tgaatttgca
    16141 atactttgcc gaaggtgacg atcaaaagtt ttcttttgat gacttcaaat cttttgtgga
    16201 atccaatgaa gaagctcaaa aatttattca gtcacaatct caaagtgttg ccgacaaaca
    16261 attggaagct tggaaacaaa ataacttaga taaaattaaa caggatacca tcaaggaata
    16321 tgaggaatct aagaaaaaca agtcacctga gcaaattcaa ctggaaaaac tacaagctga
    16381 atttgaagca gaaaaagcgt tgcgtgtgac aagtgataat aaagcatttg ttgcagaaca
    16441 aattgctgga ttagaactag atggagagtt aaaagagtct atttctcaat ttatgctaaa
    16501 taatcttgtt agttcggata cagatttcac taagaatgct gttgaaggtt ttacaagtgt
    16561 tttgaatgca attaatgaga aacatgcaga tgcattaaaa gaactacaaa tgaagtctgc
    16621 atttggtgga actcaacaat cgaataacca ggttcagcag aacaatgaaa cattaacaaa
    16681 tccagaagaa caattaggac aaattcttca acaatttaac taggagagtg aaaaattatg
    16741 aaaaaaacat ctttaaataa tttagagtat ttggatattt caccagcgat taatgctatg
    16801 caagtaccaa atacaccttt tttaagctat ttacttggtg ctggaaaaac agagccagca
    16861 aactcgacag aaattaaatg gcgtgaatat gatatcaaca acgatgattc ttctgaaaaa
    16921 cttgagggcg gagaatatcc agatgctgaa tcaggtcgaa cttggtttaa caactatact
    16981 gaaattttta gaaaatcaac ctctgtatct ggtacattag atgctattaa tgtgaatgga
    17041 gtcggaaatg aattaactaa tcaagtagca ctacgtggta tggaaatgaa aattgatttg
    17101 aaccgaaaat tgattactgg tgtaaaagct gatgaaaatg gttctaaagg tcgtcgaatg
    17161 aatgggattt tgaacttgat caattcagca aataaggcag aaacagccac tgcgggtgca
    17221 gtaacaagaa aagatatcga tactttattt aaattgatgt atgaaaaagg ttatatggga
    17281 gaaaaactat gcttgatttc tccagatatg caggagttaa tgactgatga gttagatgga
    17341 aaatcaacaa aaattgttca gttcggggaa agagtaactt ttggattgca aattggaaat
    17401 atcgtgtcta attacggtac aggtattgct ctactagaac catcattgcc aaaaggaaca
    17461 attgccgcaa tcgatactaa ttatgtgaaa ctacgtccat tacgtgaatg gagagcagaa
    17521 gaacttgcaa aaacaacgga ttctagacgt atcggtcttg taggtgaata ctctcttgaa
    17581 tacaatgctt caaactctgg ggcaatttta aatttaaagt ctgaataaaa gggagttagt
    17641 actccctttt ttgataggag gaattacagt ggtaaaaaaa gatgaaacta aaaaagatga
    17701 agtcgtgaaa tatagagtag gtaaaactaa aaattttgtt gggtttgttc atcctaaaac
    17761 tcgtagattt atcacagcag attcaaataa cgaatttatc atttctatag atgataaaga
    17821 agcaattgca attttggaag atgcaattga tgttaatgaa atttaggaag tgatctgatg
    17881 gatgaatcgc taaaaacgga aatcattgag tctacaaaag aagattttcc agatttgagt
    17941 gaagaacgca taactaattt attagaaata attttgctag aaattgaatc atacaacact
    18001 tgtaaaaatg atatttcatg ggaaaagtta aaaagcgtga ttagtgaagt gttgtataaa
    18061 ataataaaaa atgaatcaga aaaaacagta tcttcagtta gacgtggcga tacaacgatt
    18121 agttatgctt caacaacaaa tgatgttagt gagttacttc taggctatgg tgatttgata
    18181 cgaagagtta ttggttgtgg aggattggag tttttttaat gaatgaagca gatattttag
    18241 aaactactta tgaagatagt tgtattattg aaagactgac ggacattgaa gattctaata
    18301 caaatattac tattcaagat tacaaaaaag tatatgataa tcctatttct tgcgctcttt
    18361 cacaaggtca aatcgatgga ctagcagtca tagaagatgg agagatggta aatgtttcaa
    18421 ctgacacata taaattattt gttcatccta agattaaact aaaaaaagga gatcgaataa
    18481 caataactca aaaggccagt ggcttaattt tttctctatt tgctactaag cctttttact
    18541 atcctagtca ttgtgaagtg aatttgatag gaagtgagaa aaatgggtaa tctcaaatat
    18601 gaatcaaatg cagaaaagat cattgagaat tttaaaaata tgactgtaat tgctcaaaaa
    18661 gaaggaatat ccttcgttaa cgattcaatg aataaagtcg ttagtttagc taaaccatta
    18721 actcctgtaa aatcaggtaa tttaaggcga ggatatcgtg tagtaaaagc tagaaaacta
    18781 tcaagcggtc gaattgttgg agaagttata aacaatgaac attattttaa atatgtagaa
    18841 gaaggtcata ggactaaaaa tggtggcttt gtcaaaggga aattcatgtt aactcgtgca
    18901 acgaatcttg caaatatgtc ttatattcct cgaagattta aacaaatggc aataaaaatc
    18961 gttaagaaag gaaagtaaca tgaaagatga aatcattgct gcaatcagta gcaaattaaa
    19021 agaaatctat cctgatggga caatatatct tgattcagtt atgcagtcaa ctaaagattt
    19081 ttatttcgtt ttatcagtaa tggaatctgg aactgaaaat gtaggaattg atattcaaaa
    19141 tgtttctttc ttaattgata ttgcattgat tgataataac cctaatagaa atttaattaa
    19201 tgaattagtc tcacgttgtg ggactttttt taatacgatt acaatcgatg agcaaatatt
    19261 atttccaaaa gcctatttac ctgatgaagc agatggtgtt caacatattc gttttacatt
    19321 agaatttcca caatatattg aatggagtga aagataaatg ggagaaaaaa gaagtaaaac
    19381 cggaattatt tctgtcgaaa aacctacttg gtttcctttg gaagatgaga cagggaattt
    19441 tccggtatat ggtactgcgt tgccaatggg aacagcagta agtatcaaac caacagctaa
    19501 ttatgaaaca acacaagatt atggggattc agtggttcaa gatcaattta cagcttttgg
    19561 tggtgcagaa gttgaactgg aagcaaatgg atatactcat aaagttttat ctacaattac
    19621 tggagggaaa attgttaaag gtggtgcctt gcgttctgga gaagatattg cgcaagatgg
    19681 tgcgtttgca tatagacgta aaaaatcgaa tgggaaatac cgttatacgg tcttttataa
    19741 aggacagttt gctctagatt cagatgaaac atcaacgatt gaaggaagta aagtaagttt
    19801 tactcatcca acttggaagg gttcatttgt agatgtacca ggacttggat atatgtattc
    19861 agttgatgaa gatgatgaag gcgtggataa agcaatgatc ggaaattggt ttacaaaagt
    19921 agcaattcca attgaagaag cagaactttc aggaggtaca gaataatgtc aaaatatcaa
    19981 acaacgatta aattaacgaa aaaagacgaa gaaggtaaat atgagcaagt acaatttaaa
    20041 tctgctgaat ttttaccagg aacagttgta gaagatgctg caggagttat ggaagaaatg
    20101 caaacagcga ctgataagca atcagttaaa aaagctttaa gtcgtgctta ttcatttatt
    20161 gcagatactt tatttgaggg acaatttact ggtgaagatt attgcaaagg aattgatgct
    20221 cgtgagattg cctcattgac aggaaaatta ttgaagtctg ttactgcagg ttttgatgaa
    20281 acttatacag agacgaaaaa aaagtaagtg aggcgctcaa gtcaccttca tttaagtatt
    20341 cgattactta ccgagaatta gatataaaga cgcaattact cgaagcaggt tggacgttac
    20401 cagaaattga acgtactgac ttggatgagt taatgcgtct ttttgctttt agagatgcgg
    20461 taaaagagca tgaagatgtt gagtactatg ataatttcac tcaattttag gaggtgatac
    20521 tttgaataac gatgacctaa ttctgaagat gatactagat gaatctggtt ttactgccgg
    20581 tatgaataat gctgtcaaaa agttgaactc ttttgatgaa acaatcgaaa gaaaaagtag
    20641 aaatagtggc agttcattag gtagtatttg gaaaatattt gctggaagtt ttctagctag
    20701 cggagtaact agaatcgtag gagctggctt tgatctaatc aagggttcca taagtggagc
    20761 ggttgatcga gtagatacga tgaataacgc tctacgaaat ttccaaaata tgggatttag
    20821 taattcagaa attatgaaga atatcggaaa gaatgggctt ttatctcaag gtattcaagg
    20881 acttcctact gctttgaatg atgcgataag tcatgttcaa cttcttgctt cttctacagg
    20941 tgatttaact cgttcaactc aaatatttaa agctttaaat gatggaattc ttggtttcgg
    21001 tggttcaact gatcaagtta atgaagctgt tattcaatta tctcaaagtt tctcaaacgg
    21061 aaaagtagac gcacaaactt ggaactcaat gatcaacgct caacttggac ctactctttc
    21121 tgctattgct aaaaagatgg gaattacaat gggagatctg aaagaaggtt tatctcaagg
    21181 taagatttct gttgaagaat tccaaaatca attaatagaa atggatacca aaggtggcgg
    21241 aggacttaaa tcattaagtc aaatcgctaa agattctact aagggaatta aaacctctat
    21301 acaaaatgca aaaacagctg ttacacgtgg tgttggtgag gtaatagaag gattaaataa
    21361 agcattagta gattcagatt taggtggatt taagggaatt attgataaag tgggcagttc
    21421 aatggaatcg ttcttaaaag taattgctgc aaatattcct acagcagtat catttttaag
    21481 taatttattt aacgaaattc aaaaatttgg ttctgctttg aaattcatga tgccctttct
    21541 tgttcctgca gctaccgctt ttggagcgtt catgtttcaa cttaaaggta taccagcaat
    21601 aataaaaagc ttcaataatt ttaagaatgc cataattggc gttggaaatt cattaaagat
    21661 tatgggggca atagctgctg ctaatccatt tgttttaatt attggtgcag tcgttggtgc
    21721 aatagctgtt ttcggttact ttatggcaac caatgaagag cttagaaaca aggttatatc
    21781 tatttggaat gatgtgaaag attccgtaat tggtgtatta aaaaatataa aggactgggg
    21841 aattgatact tggaattctg ctaaagaaat ggcatcaaat gcagttgagg gtgtcaaaga
    21901 tgcttggtca ggaacaaagg aatggttttc taacacctgg aaaggtatta aggatggggc
    21961 cacaggttta tttgataaaa cagtagagac ttcaagaaat gcagtcgata gtgtaaaaaa
    22021 tgcatggtct aatatgaaga aatggttttc tgatacttgg caaagtataa aagattcagc
    22081 cagagaaaaa tggaatggaa tcaaaggttc tattatgtca gttgctggtc cattgattac
    22141 aggtataaag aatgcatttt tacatgttac tttttatcta gagaccttat ggaataattt
    22201 agttgagatt ggtaaaaacg tctttgagat tttaaaaaat gttatacttg cgccggtttt
    22261 gtttattacc tccatgattt ctggtggatg ggaagaaaca aaaaataata tgattgctgt
    22321 ttggaataat attaaagaaa gtgccataaa tatttgggaa tctataaaga atatatttgt
    22381 aagttatttt acaaatattt attttgctgc actcaatatt tggacagggt tcaagcttac
    22441 attgataaat atttggaatg aagtggtaaa tcaagctaaa tcgatttggt taaacataaa
    22501 gtattttttt attaatcttt ggattgatat aaaatatttt gcaattcaga aatggattga
    22561 attgaagttt tctattattc aaacttggat cgatttaaaa tataatgcca ttactacttg
    22621 gaataatatt aaacagtttt tcaaagatac ttggaaaaat attaaagata cggcatacaa
    22681 tacgtggatt tctataaaaa ataccatgat taatacatgg aataacatta aggactcttt
    22741 ctggaatatt gttactggaa ttgttaattc tgctgaaaat gcatggacaa atctaaaaaa
    22801 tggtgtttca aatgctatta atcgggtaaa agaaatcttt aattcgttaa gggaagtcaa
    22861 tttatttgaa attggcaaga acatcattga tggacttata aatggtgtaa aagaaaagtg
    22921 gaatgctttg aaagatacta tcaaaggtat tgcaggtaat atcaaaaatt ggattaaagg
    22981 agctttagat attcattctc catcaagatg gatgcgtgat atggttggta aaaatattgt
    23041 tcaaggtatt atcgttggta ttgataaaga gcaaagtaag ttagatcaaa ccatgacaaa
    23101 tttagtaaaa acaccgtctg ttcaacctgt agtaaatgga ataagtcacc taccaattac
    23161 acaaacaaag caaaatatat ctgctagtga aacaaaagaa attcatttac atttaaatgt
    23221 ttatggagat ttacctgatt caatggttcg gaagattacc acaaaaatta aaacagaatt
    23281 gacaagacaa atgaaacgag atgctggtgc agtaggagga acattatatg caacttaaga
    23341 gaggacaatt ttttattaat caacattatt cttctgaatt taatgtgtat attcaaaaca
    23401 ggcctgcttc tgtttcagct agtcgagtaa ttgaattgag agagcgagaa ggaaatgatt
    23461 ctatcattat tgataaaggc tattacaaaa atgtgactag aaagattgag tgttactata
    23521 aagcaccttc aattgatatg gtgcaggagt gggaagatcg aatcactgaa tggttagata
    23581 tgtcgtctta tagtgatttt attctttact atgatgaaca atatatttat caagctgtag
    23641 taatagaagg acctgaattt aaaggaacaa gaaaaacagg aaatattgtc ccttttgaat
    23701 ttacagtgag tattcgacca tttaaggaaa attacaatgg tagatttact attcgacaaa
    23761 cagaaacttt tgaaatttat aacccagaga agtattcttc aaaaccgctt attaaattga
    23821 gtggttctgg agatgcttct ttttatatta ataaggataa atatgatttg aaatcgttgg
    23881 acagagaatt atttatagat tctaaattag aagaggctta tcgaaaatta gatggtaatt
    23941 tagaacatca ggatcaggtc accttatttt tagattttcc atttttatat cctggaaaaa
    24001 atgaaattaa atggacgaaa aatattcatt catttgaaat aatgcctagg tggtggagaa
    24061 aagtatgaaa ccaagaatat atagtcctac tgaaacagat ttctcaacga atggcttagg
    24121 aattttaaag gattgtacaa gatgtggaat atatgaagta gctaatggaa aatatgaatt
    24181 agagttggat tatcctttag gaactagatt tgatgaatat tttgaaaatg actatcaaat
    24241 aaaagcaaag ccaaacgatc aagaagagta tcatatcttt tttattgatg ataaagatat
    24301 agatactttt ttaaatacag taactattta tgctcagagt cgtacaaatc gacttggaag
    24361 acgggtagtc actcttgtgg aagtggactc taaaactggt caagaagcga tatcaattat
    24421 tgaaactaaa atggataaaa aatctgacat acgactttat tctgatatta cggctgtttc
    24481 tagcacaatc tttgaagcga gaaatgtttt aaattgtatt gctggtgaac aaggatcatt
    24541 gcttcaatat tggggcggag aaattaaacg tgaaccattt aagctttctt tgttaaagcg
    24601 aagaggtcgc aataatgttg gaacgattcg gtatggaaaa gatatgtctg gtttgaaggt
    24661 caaattagat tggacaggta taaaaacaag aattattcca tatgctgatc ctcaaagtga
    24721 agtaggtacg actaatcgaa tttatggttc gccagtagat agtgcctata ttaataacta
    24781 tcctgatgtc tatacagaac atgttcagtt tacagaagaa caaggagtaa aagacattaa
    24841 gagcttaaat aaaatagcta aaaattactt taaaacgatc aatcctggct gtgataagcc
    24901 taaagtttct attacggttg aatttgataa gttgactgat agtgaagaag cgaaagaatt
    24961 tgcgaagatt agaaattatg gtctgtttga tacatttaaa atatatcata aaaaatataa
    25021 tatttatctt gaatcaaagg ttagtggact acaatacgat tctttatctg aaaaaacttt
    25081 gaaattagaa gcaggagaca ttcaagttgc tttttatcag caacaagctg taactattca
    25141 ggataaatta aaagattatg caacgaataa ttatatgagt gattttaatg attatgtttc
    25201 ttcaatgatt acaggtcaag gcagtgcagg aggatatgta gttttatggc caaaagaaaa
    25261 accttccaac atctttatta tggataatcc agatttagaa aaggcaagag aagtactaag
    25321 aatgaataaa aatgggattg ctttttctaa gaaaggatgg cgggggcctt ttaattcagc
    25381 ttggacatta gatagtatat ttaatgccaa ttttatacaa actggattaa tcaaagcaga
    25441 tatctttcaa aattctttta acaagactgg agatgtattg aaattagtta atggactact
    25501 ccaaatttgg aacaacaaaa agaaaataat ggagttaact aaaaaaggaa tggaattttg
    25561 gaattctaag ggttcaattg gaacgattgg aacaactgat tctgttggta atccttttcc
    25621 tggggcttct actccaaccc ctattgaaga taattcttta gttattcgta caaatggaga
    25681 cggcaaatat attttgattt ctcctaaagt aggaaaagga ttagttttat taggaaatgg
    25741 taaagcaatt tattttggag acttagatgt acaaggaaaa cttacagtta aaggaaaaga
    25801 aataattcct ggacaaggtg gtggtccatc gggaggtgga gaatctactg gtgggtatcc
    25861 tagtgaatta aaaacagatg cagaaaagag agcttggaga at
//
LOCUS       DAZKOM010000026        25463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000026 DAZKOM010000000
VERSION     DAZKOM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.026"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8NF_001128"
     CDS             133..3660
                     /locus_tag="ACG8NF_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0471619.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8NF_001129"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8NF_001129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0471620.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8NF_001130"
     CDS             7448..7789
                     /locus_tag="ACG8NF_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0471621.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8NF_001131"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8NF_001131"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0471622.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8NF_001132"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8NF_001132"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0471623.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8NF_001133"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8NF_001133"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0471624.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8NF_001134"
     CDS             13000..13737
                     /locus_tag="ACG8NF_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471625.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8NF_001135"
     CDS             13751..14566
                     /locus_tag="ACG8NF_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0471626.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8NF_001136"
     CDS             14579..15226
                     /locus_tag="ACG8NF_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0471627.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8NF_001137"
     CDS             15242..15898
                     /locus_tag="ACG8NF_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0471628.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8NF_001138"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8NF_001138"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0471629.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8NF_001139"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8NF_001139"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0471630.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8NF_001140"
     CDS             18240..18758
                     /locus_tag="ACG8NF_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0471631.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8NF_001141"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8NF_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0471632.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8NF_001142"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8NF_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0471633.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8NF_001143"
     CDS             20691..21743
                     /locus_tag="ACG8NF_001143"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0471634.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8NF_001144"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8NF_001144"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0471635.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8NF_001145"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8NF_001145"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0471636.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG8NF_001146"
     CDS             complement(23383..24144)
                     /locus_tag="ACG8NF_001146"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0471637.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACG8NF_001147"
     CDS             24333..25427
                     /locus_tag="ACG8NF_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0471638.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8443 a         4420 c         5378 g         7222 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa ata
//
LOCUS       DAZKOM010000027        25358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000027 DAZKOM010000000
VERSION     DAZKOM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.027"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8NF_001148"
     CDS             complement(132..509)
                     /locus_tag="ACG8NF_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471639.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8NF_001149"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8NF_001149"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0471640.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8NF_001150"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8NF_001150"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0471641.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8NF_001151"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8NF_001151"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0471642.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8NF_001152"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8NF_001152"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0471643.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8NF_001153"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8NF_001153"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0471644.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8NF_001154"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8NF_001154"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0471645.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8NF_001155"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8NF_001155"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0471646.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8NF_001156"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8NF_001156"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0471647.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8NF_001157"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8NF_001157"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0471648.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8NF_001158"
     CDS             10661..11575
                     /locus_tag="ACG8NF_001158"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0471649.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG8NF_001159"
     CDS             complement(11708..13273)
                     /locus_tag="ACG8NF_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471650.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG8NF_001160"
     CDS             complement(13266..14717)
                     /locus_tag="ACG8NF_001160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0471651.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG8NF_001161"
     CDS             complement(14731..15747)
                     /locus_tag="ACG8NF_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0471652.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8NF_001162"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8NF_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0471653.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8NF_001163"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8NF_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0471654.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8NF_001164"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8NF_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0471655.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8NF_001165"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8NF_001165"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0471656.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG8NF_001166"
     CDS             21340..22764
                     /locus_tag="ACG8NF_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0471657.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG8NF_001167"
     CDS             complement(22806..23063)
                     /locus_tag="ACG8NF_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471658.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8NF_001168"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8NF_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0471659.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG8NF_001169"
     CDS             24337..25314
                     /locus_tag="ACG8NF_001169"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0471660.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8201 a         4317 c         5696 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcgccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZKOM010000028        24319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000028 DAZKOM010000000
VERSION     DAZKOM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.028"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..816)
                     /locus_tag="ACG8NF_001170"
     CDS             complement(<1..816)
                     /locus_tag="ACG8NF_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0471661.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTRE"
     gene            complement(1040..2980)
                     /gene="thrS"
                     /locus_tag="ACG8NF_001171"
     CDS             complement(1040..2980)
                     /gene="thrS"
                     /locus_tag="ACG8NF_001171"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0471662.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3317..3496)
                     /locus_tag="ACG8NF_001172"
     CDS             complement(3317..3496)
                     /locus_tag="ACG8NF_001172"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0471663.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3543..5366)
                     /locus_tag="ACG8NF_001173"
     CDS             complement(3543..5366)
                     /locus_tag="ACG8NF_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0471664.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5644..7923
                     /gene="helD"
                     /locus_tag="ACG8NF_001174"
     CDS             5644..7923
                     /gene="helD"
                     /locus_tag="ACG8NF_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0471665.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8016..8663)
                     /locus_tag="ACG8NF_001175"
     CDS             complement(8016..8663)
                     /locus_tag="ACG8NF_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471666.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8842..9561)
                     /locus_tag="ACG8NF_001176"
     CDS             complement(8842..9561)
                     /locus_tag="ACG8NF_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0471667.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9692..10843)
                     /locus_tag="ACG8NF_001177"
     CDS             complement(9692..10843)
                     /locus_tag="ACG8NF_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0471668.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11060..11800)
                     /locus_tag="ACG8NF_001178"
     CDS             complement(11060..11800)
                     /locus_tag="ACG8NF_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0471669.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11804..12127)
                     /gene="rsfS"
                     /locus_tag="ACG8NF_001179"
     CDS             complement(11804..12127)
                     /gene="rsfS"
                     /locus_tag="ACG8NF_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0471670.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12184..12777)
                     /gene="yqeK"
                     /locus_tag="ACG8NF_001180"
     CDS             complement(12184..12777)
                     /gene="yqeK"
                     /locus_tag="ACG8NF_001180"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0471671.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12761..13411)
                     /locus_tag="ACG8NF_001181"
     CDS             complement(12761..13411)
                     /locus_tag="ACG8NF_001181"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0471672.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13421..13732)
                     /gene="yhbY"
                     /locus_tag="ACG8NF_001182"
     CDS             complement(13421..13732)
                     /gene="yhbY"
                     /locus_tag="ACG8NF_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0471673.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13748..14857)
                     /gene="yqeH"
                     /locus_tag="ACG8NF_001183"
     CDS             complement(13748..14857)
                     /gene="yqeH"
                     /locus_tag="ACG8NF_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0471674.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14854..15381)
                     /locus_tag="ACG8NF_001184"
     CDS             complement(14854..15381)
                     /locus_tag="ACG8NF_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0471675.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15561..16346)
                     /locus_tag="ACG8NF_001185"
     CDS             complement(15561..16346)
                     /locus_tag="ACG8NF_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0471676.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16339..17208)
                     /gene="accD"
                     /locus_tag="ACG8NF_001186"
     CDS             complement(16339..17208)
                     /gene="accD"
                     /locus_tag="ACG8NF_001186"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0471677.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17209..18588)
                     /locus_tag="ACG8NF_001187"
     CDS             complement(17209..18588)
                     /locus_tag="ACG8NF_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0471678.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18596..19015)
                     /gene="fabZ"
                     /locus_tag="ACG8NF_001188"
     CDS             complement(18596..19015)
                     /gene="fabZ"
                     /locus_tag="ACG8NF_001188"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0471679.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19040..19516)
                     /gene="accB"
                     /locus_tag="ACG8NF_001189"
     CDS             complement(19040..19516)
                     /gene="accB"
                     /locus_tag="ACG8NF_001189"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0471680.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19519..20754)
                     /gene="fabF"
                     /locus_tag="ACG8NF_001190"
     CDS             complement(19519..20754)
                     /gene="fabF"
                     /locus_tag="ACG8NF_001190"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0471681.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20773..21510)
                     /gene="fabG"
                     /locus_tag="ACG8NF_001191"
     CDS             complement(20773..21510)
                     /gene="fabG"
                     /locus_tag="ACG8NF_001191"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0471682.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21522..22439)
                     /gene="fabD"
                     /locus_tag="ACG8NF_001192"
     CDS             complement(21522..22439)
                     /gene="fabD"
                     /locus_tag="ACG8NF_001192"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0471683.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22546..22770)
                     /locus_tag="ACG8NF_001193"
     CDS             complement(22546..22770)
                     /locus_tag="ACG8NF_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0471684.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22823..23785)
                     /locus_tag="ACG8NF_001194"
     CDS             complement(22823..23785)
                     /locus_tag="ACG8NF_001194"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0471685.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23795..24235)
                     /locus_tag="ACG8NF_001195"
     CDS             complement(23795..24235)
                     /locus_tag="ACG8NF_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0471686.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6647 a         5424 c         4239 g         8009 t
ORIGIN      
        1 ttctcgtgtt tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa
       61 aatgatatcg atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt
      121 gatttctttt ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt
      181 tccaaattgc gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc
      241 gactacatct gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt
      301 gctgacaagt tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac
      361 acgagaccat ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa
      421 aaaaacagtc tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag
      481 aatatctact gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga
      541 gtaaaaggac tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc
      601 tgttgaaatc gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg
      661 tacaactgtc ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg
      721 gaccttctca atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc
      781 ttccatcata ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat
      841 agtctatgta agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca
      901 aaaaaacaat ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca
      961 tctgtgtctt tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt
     1021 tttacaggaa atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag
     1081 catcaataaa gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt
     1141 tcactgttgc atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg
     1201 tttgagaggc acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca
     1261 accccttcat ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag
     1321 aaacagggat gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata
     1381 cttctgttaa ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta
     1441 ctggacgatg cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca
     1501 ataagaagtc taattgtata gttgataatg tttcttctgt accaagagct gttttgactt
     1561 gaacatctag ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt
     1621 ctaattcatc catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat
     1681 caaaatattt atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa
     1741 tattgaagtc agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga
     1801 tttgatcagg acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta
     1861 aacctgacaa agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa
     1921 ttggtaattc acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt
     1981 tcattgggcg aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt
     2041 cttgataatg atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg
     2101 tatatacatg ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc
     2161 gtcggattgt tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa
     2221 ccatgaacaa gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac
     2281 ggagacggat atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt
     2341 gcatcatttg attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa
     2401 atacttgtat acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc
     2461 cttgttgata agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt
     2521 acggatcact tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg
     2581 gcagattttc tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag
     2641 ttacttggtt ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa
     2701 cgccaaagtg gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg
     2761 aatgacggac taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat
     2821 cttcctcgat tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt
     2881 tttttgctaa gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa
     2941 attctttgac tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat
     3001 gttttgattg atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag
     3061 agatactagg acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca
     3121 agcgttgtaa cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt
     3181 ggttttctcc tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt
     3241 cagttggctt tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc
     3301 aagtgggaaa ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca
     3361 tctcattgat gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt
     3421 ccgtgatatc tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga
     3481 catgtacgaa tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc
     3541 tatcatacta tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct
     3601 ctcaaggcac tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat
     3661 gcggggatta gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc
     3721 agtacagact gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca
     3781 cgcaagagat catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca
     3841 tgagccccca ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg
     3901 tctcctacca tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta
     3961 tcttgaggca agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga
     4021 gcaatggcag atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc
     4081 tccttgattg tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca
     4141 aaagatcgat caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa
     4201 acaaaagagg cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga
     4261 ccggtcactt ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact
     4321 aacgaacgag ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc
     4381 tcttcttttt ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta
     4441 agcgttcctg ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt
     4501 ccagttttta caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg
     4561 ggcgcagcta gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa
     4621 gggtcttttg agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg
     4681 actgcataac gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact
     4741 agtttaataa tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc
     4801 gatccgtctc catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg
     4861 cgggactcac ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca
     4921 tctgcgggta caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg
     4981 attactatat cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct
     5041 aacaaagact gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg
     5101 cccgtaagca tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca
     5161 aggattgcgg taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc
     5221 atttccaaaa acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca
     5281 agggccggct ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta
     5341 agtaaaaatt ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg
     5401 ttagtaataa atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt
     5461 taaaacaata ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag
     5521 ttctacaaac aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc
     5581 ttttttcgaa aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat
     5641 gcgatgaatg agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag
     5701 atcgaacaaa atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg
     5761 acaaaagaca cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct
     5821 gctgttgaat atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca
     5881 cagcaaaaac gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc
     5941 gactttaaag aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat
     6001 ctggcagaag aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa
     6061 ggtgagattg gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag
     6121 ttgaaacgac aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa
     6181 gtaatcaatg atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa
     6241 aacatcgtct ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa
     6301 gtaatgctga ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt
     6361 gcttttctgt tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca
     6421 cccaaccatt tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc
     6481 gtgcctacac agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta
     6541 gatgaacagc aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg
     6601 aaaagcggat tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac
     6661 ggtcccttgt tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt
     6721 cgccaatggt ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta
     6781 caaacgaagc tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg
     6841 gtcaaagatc tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa
     6901 tatacagaac aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa
     6961 agattccgtc gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa
     7021 tatctccact ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag
     7081 accaggtggc aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt
     7141 caagaggatg ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca
     7201 cacaaagctc gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca
     7261 ttgctgcaag ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat
     7321 gtctttggaa atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc
     7381 actcgtttcc aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat
     7441 cattttattg tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg
     7501 ctcatccaag gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg
     7561 actcagggaa aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc
     7621 gaagctcttt atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat
     7681 gagagtgact ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg
     7741 gagtttgacc gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac
     7801 aaattgatcc tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc
     7861 aataatgaaa gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg
     7921 taagtaaaaa gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata
     7981 attgcagaaa aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct
     8041 gtttcaggat attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct
     8101 ggatattctg ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg
     8161 taaattcgat tccattcaat gaattggtca attgattgga aattgctgat tggatagccg
     8221 ccacaatcca aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt
     8281 tttttcgtgt ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag
     8341 ctccactcta atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag
     8401 gctgaatcag aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt
     8461 ggtgcgcggt ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt
     8521 cctaatcgat agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca
     8581 gttacgttca cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt
     8641 tctaaagtga tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat
     8701 ttcacatgat acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca
     8761 cacaaaaaag caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata
     8821 cttttcgtta aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac
     8881 atcttctaga tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga
     8941 tagtgttacc tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc
     9001 tttttctttt aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat
     9061 ttgtccttct tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag
     9121 gatctcgtca gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga
     9181 aaccgctccg gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc
     9241 tgctgttcga ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc
     9301 aaatccttca tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat
     9361 tgctcggtca ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg
     9421 tagtttttgg tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat
     9481 cccaaatttt gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat
     9541 atttgcccat ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc
     9601 aaaaacgtcc tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct
     9661 tccttcattc ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat
     9721 tctgttccgg aatattgcct cccaattgat agatttgatc acttctaact aacagttccg
     9781 cttcttgtgc ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta
     9841 agaatcttct tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga
     9901 caggaatctc ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg
     9961 tccatgtata tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg
    10021 cagctgctgt tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt
    10081 ccagcaaagt agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct
    10141 gcagcgaaca agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt
    10201 ttccttcttc taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga
    10261 ttttttcatg atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat
    10321 acttggcatt ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa
    10381 gcgtaatatc gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg
    10441 tttgatcaga cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc
    10501 gtccgaaact gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac
    10561 agtccagtgt ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg
    10621 cccattcgac gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc
    10681 gatgccattt atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag
    10741 caatgacgac ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat
    10801 tgtgaaaagg attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata
    10861 gttatcgata aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc
    10921 gaactatata aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag
    10981 agcaatgcct atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta
    11041 actcgagcag gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct
    11101 ctgatgctgc ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt
    11161 ccagcattct taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac
    11221 gaataaatcg accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc
    11281 tgtgtttctc gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat
    11341 gatagctata ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa
    11401 atcggccatt ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc
    11461 tatttttcat atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt
    11521 ctgataaatc gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc
    11581 gctggctggc aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat
    11641 cttttgcaaa ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg
    11701 tgtcttcttg taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca
    11761 tcacttcatc gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac
    11821 taagatcgac tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg
    11881 gcgcatggaa aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt
    11941 cttttccttc aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt
    12001 ctgtaattgc gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca
    12061 acaaggaaac atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg
    12121 cttcaatcgc aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc
    12181 agcttatttt tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg
    12241 ttcttgcgca agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag
    12301 atcagcccat gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat
    12361 atagtctgct acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg
    12421 cacccggatt gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga
    12481 agcgccaaca agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc
    12541 gtctcttacg attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt
    12601 caatgcagca atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc
    12661 tgtttcttct actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt
    12721 ttgcatcagg ttttctctag aataagcagt atattcatta ctatagtcca tattcataca
    12781 acccttcatt ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc
    12841 cttctttgat cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc
    12901 aaattacagg atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga
    12961 ccatagaagt cagctcatcg attttatacc attttggcaa atattctacc atatccccac
    13021 cgataataaa gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat
    13081 aggtgtaact tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt
    13141 cttccaccgc taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat
    13201 caacatgagg tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc
    13261 cagcttgttc agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga
    13321 tccctacttg ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac
    13381 gaacaaatgt cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt
    13441 ttgatagcct ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa
    13501 tgatttggac gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa
    13561 tctcatctgt attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt
    13621 caatttgaac gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa
    13681 gatgatgcgc tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc
    13741 ttcttcctta aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg
    13801 caattactac tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt
    13861 tttctttcac agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac
    13921 gaggaggctg taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt
    13981 tcgttcgatg aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc
    14041 tgataaaatc aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat
    14101 aaactttagg tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt
    14161 agtgcgccat ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat
    14221 ctaaaggaat ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat
    14281 cttgtactcc cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg
    14341 ttactccgac gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt
    14401 ccgtcatctc atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg
    14461 cttcgtaagc acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta
    14521 atatatccac tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc
    14581 ctgggattag tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt
    14641 tttgccctaa ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg
    14701 tctcattata atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac
    14761 ctttttccaa agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa
    14821 cggcaccaca gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat
    14881 atcagggtgt tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg
    14941 tgtcttccaa ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg
    15001 attggctcca cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc
    15061 taaatccaat tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc
    15121 aacatacaaa agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga
    15181 aacaacaaca acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc
    15241 tgttccatct ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt
    15301 gatgcctaga ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt
    15361 tggtttgtaa tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa
    15421 gtactcctca accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt
    15481 cgtctttcag aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa
    15541 aatttagaaa caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat
    15601 ttatgctgat ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg
    15661 tgtgcgactt tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca
    15721 acgattccta actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg
    15781 gcgcgactac cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa
    15841 atcgtttctt ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt
    15901 ccaccttcac caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga
    15961 tttctggcaa tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct
    16021 gctgtattga tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt
    16081 aaagcttttc gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc
    16141 agatcccgtc ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt
    16201 ccaccaacaa tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa
    16261 tcgaagatct cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga
    16321 acgacatcat gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag
    16381 aagcgtatgg attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg
    16441 agataataag aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc
    16501 gatgacgcgt cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc
    16561 ttccattgca aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata
    16621 aaaaagacct tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag
    16681 agaaaagatt ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag
    16741 actttccttt gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat
    16801 acttcccatg ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt
    16861 tgctttacca gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt
    16921 ttccgtatat tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc
    16981 aaaacttttt tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta
    17041 tccgcaatga gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca
    17101 atttggacac tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc
    17161 tctattagga ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc
    17221 tatctccgtt tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta
    17281 atcacctgct atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat
    17341 gattccttct gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg
    17401 cgtatttcca tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata
    17461 tcccgggtaa actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt
    17521 ttggatcttc cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca
    17581 ttcgattgcg tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc
    17641 tgctatctcc acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg
    17701 ttccacttgg attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag
    17761 aaactcgatc gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc
    17821 tcctagtgct tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt
    17881 ttgattgttg cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg
    17941 atcacctaag atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat
    18001 atcatcattt ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag
    18061 ctcttctttt gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag
    18121 catcactgga taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac
    18181 accagtactt cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat
    18241 cttatttccc attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc
    18301 ttcacacatg gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc
    18361 ttcggctttt gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac
    18421 ggcttttgcc gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc
    18481 atctgcttct gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat
    18541 acgtacagcg atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc
    18601 caatcataaa ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt
    18661 tgcctattcc ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc
    18721 ccggtgtaac tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc
    18781 ccttgaattg ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa
    18841 tcaatacccc tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga
    18901 tcgttacatt tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa
    18961 tcaaaagcat tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt
    19021 tccctctttc ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga
    19081 caactgactc gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct
    19141 cattcatcag tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac
    19201 ctactttttt gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg
    19261 gcgaaacaac ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag
    19321 cagttgttgc ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg
    19381 tttgaaccat attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac
    19441 cttcacgcaa atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga
    19501 tttcattgat atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc
    19561 gccaaaccct aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt
    19621 gacaactgtc aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa
    19681 ttgatgttct aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa
    19741 atgtccagtc atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc
    19801 gatttggatt gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt
    19861 gatatatccg acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt
    19921 agatgcacca gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc
    19981 gtatccaacg atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc
    20041 aagaactaga acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg
    20101 aatcgaacca cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc
    20161 aatccccatc tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc
    20221 atgtttgatg ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac
    20281 gatagctgta caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt
    20341 ggcaatcgcc attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg
    20401 aataacttgg ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa
    20461 ccggtcttga ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc
    20521 gtagattcca tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt
    20581 atcgtaaggg aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa
    20641 tttagtgatc ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc
    20701 gtcatttcca ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt
    20761 gctctccttt tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt
    20821 gatatatgga ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa
    20881 acgctgcatc ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt
    20941 catatcagtt gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc
    21001 ttctcgtgcc acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc
    21061 ttgaccaata tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg
    21121 ctgcttcatc atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat
    21181 atccagacat tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat
    21241 tccggcattg tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat
    21301 ttgtcccgct ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa
    21361 tgcttcgatt tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt
    21421 tgctccagct tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt
    21481 gataaataca ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt
    21541 tcatcaaatg tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc
    21601 ataaatccgc ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt
    21661 tttaacttat ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt
    21721 cccgcgattg tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa
    21781 ggttcagaga attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa
    21841 atagctgtat gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct
    21901 tttaatagta aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga
    21961 ggtgtattat aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc
    22021 tcgatcgttt caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca
    22081 gcttctgtca tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc
    22141 gcattactag ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg
    22201 atgattccat gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc
    22261 tgtgtatatt gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg
    22321 tagccgagaa tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa
    22381 tcttttccca taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg
    22441 tttttctcca atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca
    22501 ttcctatttc aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt
    22561 ttctacgtaa gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac
    22621 gtcaaattca tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa
    22681 gtcttcttgg atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc
    22741 gatgatttct tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt
    22801 tttattttta taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc
    22861 cgccatagcc tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa
    22921 ctgcttcatc caataagata gggatgcttg cagcagatgt attcccatat ttatccatat
    22981 tggtcaggaa cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga
    23041 ggtttgcctg atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat
    23101 tgtttgcaac gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat
    23161 acgaaccttc tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca
    23221 atgagctact tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat
    23281 tcgcttctaa gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag
    23341 accaatcgat tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc
    23401 agcggatcat tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga
    23461 tatcaaaagc gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg
    23521 gtgtcgtgta atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta
    23581 gatttttctg ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat
    23641 ttgttacaca tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat
    23701 ccatcatctt ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg
    23761 cagtaatcat tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt
    23821 gcaagttttt cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga
    23881 tgttcttgac catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt
    23941 tcgttagtcc caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc
    24001 ctttttttac taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt
    24061 tggctacttc tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct
    24121 ctgtgattga cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa
    24181 tatcattgaa gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc
    24241 accgtccact aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt
    24301 tttcaaaatg caaccttaa
//
LOCUS       DAZKOM010000029        24258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000029 DAZKOM010000000
VERSION     DAZKOM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.029"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8NF_001196"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8NF_001196"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0471687.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8NF_001197"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8NF_001197"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0471688.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8NF_001198"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8NF_001198"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0471689.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG8NF_001199"
     CDS             complement(3374..4681)
                     /locus_tag="ACG8NF_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0471690.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG8NF_001200"
     CDS             complement(4684..5265)
                     /locus_tag="ACG8NF_001200"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0471691.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG8NF_001201"
     CDS             complement(5837..7129)
                     /locus_tag="ACG8NF_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0471692.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG8NF_001202"
     CDS             complement(7126..7749)
                     /locus_tag="ACG8NF_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471693.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG8NF_001203"
     CDS             complement(7752..8588)
                     /locus_tag="ACG8NF_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471694.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG8NF_001204"
     CDS             complement(8605..9492)
                     /locus_tag="ACG8NF_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0471695.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG8NF_001205"
     CDS             complement(9508..10791)
                     /locus_tag="ACG8NF_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0471696.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG8NF_001206"
     CDS             complement(11474..12157)
                     /locus_tag="ACG8NF_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0471697.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG8NF_001207"
     CDS             complement(12175..12792)
                     /locus_tag="ACG8NF_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0471698.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG8NF_001208"
     CDS             complement(12802..13626)
                     /locus_tag="ACG8NF_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0471699.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG8NF_001209"
     CDS             complement(13619..14179)
                     /locus_tag="ACG8NF_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471700.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG8NF_001210"
     CDS             14353..15309
                     /locus_tag="ACG8NF_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0471701.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG8NF_001211"
     CDS             complement(15614..16570)
                     /locus_tag="ACG8NF_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0471702.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG8NF_001212"
     CDS             16674..17276
                     /locus_tag="ACG8NF_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0471703.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG8NF_001213"
     CDS             complement(17364..18863)
                     /locus_tag="ACG8NF_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0471704.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG8NF_001214"
     CDS             complement(18879..19403)
                     /locus_tag="ACG8NF_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0471705.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG8NF_001215"
     CDS             19701..20450
                     /locus_tag="ACG8NF_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0471706.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG8NF_001216"
     CDS             20441..21226
                     /locus_tag="ACG8NF_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0471707.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG8NF_001217"
     CDS             21195..21911
                     /locus_tag="ACG8NF_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0471708.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG8NF_001218"
     CDS             complement(22013..23032)
                     /locus_tag="ACG8NF_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0471709.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACG8NF_001219"
     CDS             complement(23051..24052)
                     /locus_tag="ACG8NF_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0471710.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4224 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcgactga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAZKOM010000030        24219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000030 DAZKOM010000000
VERSION     DAZKOM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.030"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            345..509
                     /locus_tag="ACG8NF_001220"
     CDS             345..509
                     /locus_tag="ACG8NF_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471711.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            506..736
                     /locus_tag="ACG8NF_001221"
     CDS             506..736
                     /locus_tag="ACG8NF_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471712.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            921..1466
                     /locus_tag="ACG8NF_001222"
     CDS             921..1466
                     /locus_tag="ACG8NF_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0471713.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1563..1784
                     /locus_tag="ACG8NF_001223"
     CDS             1563..1784
                     /locus_tag="ACG8NF_001223"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0471714.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1941..2105)
                     /locus_tag="ACG8NF_001224"
     CDS             complement(1941..2105)
                     /locus_tag="ACG8NF_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0471715.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2207..2728
                     /locus_tag="ACG8NF_001225"
     CDS             2207..2728
                     /locus_tag="ACG8NF_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0471716.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2715..3155
                     /locus_tag="ACG8NF_001226"
                     /pseudo
     CDS             2715..3155
                     /locus_tag="ACG8NF_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3195..3353)
                     /locus_tag="ACG8NF_001227"
     CDS             complement(3195..3353)
                     /locus_tag="ACG8NF_001227"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0471717.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3446..4207
                     /locus_tag="ACG8NF_001228"
     CDS             3446..4207
                     /locus_tag="ACG8NF_001228"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0471718.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4394..5746
                     /locus_tag="ACG8NF_001229"
                     /pseudo
     CDS             4394..5746
                     /locus_tag="ACG8NF_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5762..6982
                     /locus_tag="ACG8NF_001230"
     CDS             5762..6982
                     /locus_tag="ACG8NF_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0471719.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7017..7694
                     /locus_tag="ACG8NF_001231"
     CDS             7017..7694
                     /locus_tag="ACG8NF_001231"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0471720.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7691..8884
                     /locus_tag="ACG8NF_001232"
     CDS             7691..8884
                     /locus_tag="ACG8NF_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0471721.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8899..9225
                     /locus_tag="ACG8NF_001233"
     CDS             8899..9225
                     /locus_tag="ACG8NF_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0471722.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9229..9564
                     /locus_tag="ACG8NF_001234"
     CDS             9229..9564
                     /locus_tag="ACG8NF_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0471723.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9561..9956
                     /locus_tag="ACG8NF_001235"
     CDS             9561..9956
                     /locus_tag="ACG8NF_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0471724.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9953..10324
                     /locus_tag="ACG8NF_001236"
     CDS             9953..10324
                     /locus_tag="ACG8NF_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471725.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10340..10900
                     /locus_tag="ACG8NF_001237"
     CDS             10340..10900
                     /locus_tag="ACG8NF_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0471726.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11025..11336
                     /locus_tag="ACG8NF_001238"
     CDS             11025..11336
                     /locus_tag="ACG8NF_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471727.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11360..11551
                     /locus_tag="ACG8NF_001239"
     CDS             11360..11551
                     /locus_tag="ACG8NF_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471728.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11551..15399
                     /locus_tag="ACG8NF_001240"
     CDS             11551..15399
                     /locus_tag="ACG8NF_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0471729.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15400..16272
                     /locus_tag="ACG8NF_001241"
     CDS             15400..16272
                     /locus_tag="ACG8NF_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0471730.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16269..17318
                     /locus_tag="ACG8NF_001242"
     CDS             16269..17318
                     /locus_tag="ACG8NF_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471731.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17322..18146
                     /locus_tag="ACG8NF_001243"
     CDS             17322..18146
                     /locus_tag="ACG8NF_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471732.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18164..20224
                     /locus_tag="ACG8NF_001244"
     CDS             18164..20224
                     /locus_tag="ACG8NF_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0471733.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20241..20390
                     /locus_tag="ACG8NF_001245"
     CDS             20241..20390
                     /locus_tag="ACG8NF_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471734.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20387..20833
                     /locus_tag="ACG8NF_001246"
     CDS             20387..20833
                     /locus_tag="ACG8NF_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471735.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20835..20972
                     /locus_tag="ACG8NF_001247"
     CDS             20835..20972
                     /locus_tag="ACG8NF_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0471736.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21007..21249
                     /locus_tag="ACG8NF_001248"
     CDS             21007..21249
                     /locus_tag="ACG8NF_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0471737.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21265..21462
                     /locus_tag="ACG8NF_001249"
     CDS             21265..21462
                     /locus_tag="ACG8NF_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0471738.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21473..22489
                     /locus_tag="ACG8NF_001250"
     CDS             21473..22489
                     /locus_tag="ACG8NF_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0471739.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22604..23812
                     /locus_tag="ACG8NF_001251"
     CDS             22604..23812
                     /locus_tag="ACG8NF_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471740.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            23796..23966
                     /locus_tag="ACG8NF_001252"
     CDS             23796..23966
                     /locus_tag="ACG8NF_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471741.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT         8452 a         4061 c         5289 g         6417 t
ORIGIN      
        1 agaaatgatt ccattaataa tttcaatttt tgcgctttgt cttaatgtct atatgattgg
       61 atttaaaaat gggcaaaata aaagatagta gtaactaaga aagaaatttt tatattgtca
      121 ctgtggcgga aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaactatgc
      181 aatgttcgat tcattgccag tgactttggc aaccgaggca tcggcggttt aaaaatatag
      241 gggtgcgcaa tttcgtacgc gttttgtgca tcgtgtaggt tgctattaca tattagatca
      301 ctcgttgagt ggtcttttta ttttacataa aggaggtaac aacgatgtat agaccacaat
      361 acttagaaca gaagtatgaa gtaatcactg tgcaaaacgg taacggtgag atagtacgaa
      421 agtatagaag accaataaag agcgatacat ataaacgaaa ggaaaacaat gaagttattt
      481 cttttcgcag aaggagaaaa gccaaatgag aaactactgg tatgtatcgc taactaatga
      541 atatcctcga accattgatg attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg
      601 gaagtactct attgtcgaaa tgctaagaga agctacacca aacgaagtgg ataaatgcaa
      661 gctgatatat tgcggtcatg gctattggaa agacgagtat atccaacaaa acattgagag
      721 gtggatagat agatgagtta cctcgaacat ttgaaacgtt gctacatgca ttctaagaat
      781 aaacttcctg acagctacac tgtagatgat gttgctatac atgtattgaa gactgagagc
      841 catagcagtc ctgatggtag cagtaaggag cagacgctcg cgtggttcaa gttctttaag
      901 tggattaagg aggaagagta atgcacatgc acataggaga aaagatatat aaattgagac
      961 ggcttaataa cgaaacaatg aacgacttag ttaaacaaac tggagtgtca aaatcatggt
     1021 tatctgatat tgagtctgga aaaaagcaac gcgttgattt taataagata tgtgcaatag
     1081 ccaaacatta taaagttagc ttagaatact ttcgggatga ttatttggat agtgatgaac
     1141 acgaggatat tattcctttg acaggcgaaa caagagttaa atcagtgaaa tcgcttgaac
     1201 aaagcctaaa gtatctgaat aacaaacaag aacctaacct tctagagatc aaactaaaag
     1261 atactgactc agtaccagag gtttggtaca agggtgagag gttggatgaa ttgcctaaag
     1321 gattagtaga tgtctcgtat cattggaaga ctgatgattt tactaatgat gatagaggag
     1381 cgaacgacat cacgattcaa tacttttcta gctttaatga taagtatcca gataagaaaa
     1441 caatcggaca caagagagat atgtaaatga aagaagttag acctagagac gaaatagaca
     1501 aactatacaa gaccaaacga tggcgagacc taaggcaagt agtaatagct agggacttcg
     1561 gcatgtgcca agagtgcaag cgtcgagggc ggaacacaag gggcacgatc atccatcaca
     1621 tagtcgaggc gagggaagac ctgtcactgt tctggtccgt agataacctt gaatgtatct
     1681 gtgtagcttg tcacaacaga gagcatccag agagatcagg cgggaagaag aaaccaaaac
     1741 ctaaatcaca tatcgttaaa atgtattcaa ctcctgaaag ataagtttgc agcgaaatga
     1801 aggtagcccc cctactctaa aagattaaag agtaaggctt gagaagaacg gtgctgtcct
     1861 tccttcgtaa aaagaccgct tttcaagttt tttggagaaa aaggaaaaag ccgaccaatt
     1921 taagccggct ttggacgaag ctatttctta gtccatttgt ttcctttttg agaagtaggg
     1981 ggtaatcggt cgcctggatc aatttttact tcttgtccgc cttggacatt tccaccacga
     2041 ggacccactt ctttgtaggt tccttttggt ttgttgtctt cgccgggttt atagagttct
     2101 cccataggaa tccctcctta acaaatttcg gcacagcact gccgataacc taattataag
     2161 gattgtgata acgatttcaa tctatctttt gaaagaaggt gatattatgc cgcaaccagc
     2221 gaagagtgca aaattacaat tattaaacgg aaacccaaat aagaagaata ccgaagaact
     2281 ccgcaagcga gcggccgcag aagacaaatt aaaaatggct actgacaaaa tcaaaccgcc
     2341 gttatggcta gattcgctag gaaaggatac ctttgagttt atcgccgatg aattgctgtc
     2401 tgtggattta atcagtaatc cggacgtcca tacaatggct ctatactcca attggtattc
     2461 gcaatacgtt tctttagaaa aacagcttcg aaaactacaa cgagagtaca agttgaacta
     2521 tgcgcttgcg aaaaaggagg cagaggcgag aggtgagccg tttaatgaac ctaatgaatt
     2581 aattggtaat ccgctctctc ggcagatgga tacagcgtct cggaatctcc gttcttttgg
     2641 cgctgattta ggactatcac cagcagccag agctaagtta gctattaaga tggctgatga
     2701 tggtggtgat gacgatgacg acttctaata ttttggatat gtcttacaca gaacgtgtgg
     2761 actattggca aagctatctt gaggagcaag cttcttgggg tggcttttta aaatgtccat
     2821 atccggaatt gttaactact tggtatgcgg aacgattaat cgatggaagc ataccagcca
     2881 gcaaagaaaa tattcaagct gctaaacggc atatgcgtga tttgcagcgc caaggaacag
     2941 atgattttcc ttggatcttt gacgaagaaa aaggtcaccg gcctattaga tatatcgaaa
     3001 aaaaatgtaa accaactgaa ggcgactttg gttcgtttgt tttgcaacct tggcagcatt
     3061 tcataattgg atccatgtac ggatgggtac atcgtgatac aggagagcgt cgcttccgcg
     3121 aggctcttat ttttgttgga cgtaaaaacg gtaagttttg ccgtttaaaa atcgggcaaa
     3181 atcggtgaaa acctttattt ttggctttct tttagtaata attcgatagc tttatcaagc
     3241 aatttagaca taggaaccat agtttcttcc gacatttttt ttaatccttc gtataaatct
     3301 ttatcaattg cgttagaaat tctaattctg ttttttagtc cgtatttgtc catgtgtgac
     3361 actccttttt attttactat aacatccgat tccaccgctt gcaactacca tcaattgatg
     3421 gtagtatata aaagaggtga gtagattgaa aaaatacatt atttacaaac acttaaataa
     3481 aataaatggc aaaatttata tcggtgtcac aaatgatatt ggcagaaggt ggaggagcgg
     3541 tgggattgaa tataaaccac caaagaacga aacccaacac cacagaagtt tttggaatgc
     3601 aatacaaaaa tacgggtggg aaaattttga tcatttgatt attgaagaag acttaacgat
     3661 gaaagaagct tttgagaaag aaaagtttta tattgaactt tatgactcaa ctaataagaa
     3721 aaaaggttat aacatcgcaa aaggcggaaa tggcggaata atatataaag ttcaccccaa
     3781 aggaatgcta gggaaaaaac aatctaaaga attttcttca aatcacagta aatgggcaaa
     3841 gaaccataag aataattgta tgacaaacgg tgatgttgta tggggtgtta ctcacgaaca
     3901 cccaaaaggc atgttgggaa agcaccacag caaagaatca attatgaaaa agaaagcata
     3961 tagtggtgaa aatgctgtaa cctcaaaacc aattgtcgca attgaacggg atggaactaa
     4021 aagagaattt cacagcgcta agctttgcat ggcttactat tcgattagta catgtgtttt
     4081 ttaccgactt ttaaaagatg gcgcccctta tgtgataaat cctaaagcga actacagaaa
     4141 taaagaaaaa atattagcta tcgaaggaat tatgttcaaa cataaagaag ataccgaggt
     4201 aagttagtag attgcgaaag gttactaact accgtaacgc gtaggagttg aataaatata
     4261 atgctcccaa gagtgtccga cagcagataa tttgctgaaa atgtacgcta aactgggcca
     4321 gaactgactg accgatgaaa atgagggtga cctccagagt gcgagataaa aagctcgcag
     4381 ataataacaa tcgaaaacat cactaatttc aggcctttcc acatacatgg tcgcttatga
     4441 tgatgaacaa ggcgccaacg tttacgtatt ggcaaatgct cgtgatcaag caagcttgtt
     4501 gtttgataag gccgcagaaa tggtcaaaca atcgccggcg ctctttaaga aatttggtaa
     4561 gcctaaacga tcaagtatta attatgctcc cgccttttct aaaatggaac cacgcgcctc
     4621 agatagccgg aaattggatg ggctaaacac tcactttggt atttttgacg agatccacga
     4681 gtttacgaat tacaatctga tcaacgttat caagaaatca agaggaacca gaaaacagcc
     4741 tctgatagtt tatatcacaa ctgctggata tgtattagat ggtccgttga tgtcttattt
     4801 tgagcaaggt gtggactgtt tggaacattt ggaagatgac atcgatgaac ggactttcta
     4861 ttatctggca aaacttgaca gtgcggaaga ggctgatgac ccaagattat ggatcaaagc
     4921 caatccgaat atttgtctaa tgaattttgt tggcatgcta gatgactatg ttaaggataa
     4981 aaaagatcca aaagaatatg ctgactggat taccaagcaa tttaacttgt tttccgatat
     5041 cgatgagctg tcatttgtcg atatgcctac cattaaacga aacaataaaa ccatcgatat
     5101 tgaaacgctc aaaggtaaga agtgtgtcgg tggttttgac ttgtccgaaa cggaagactt
     5161 taccgcagcc gttttagaat ttccgcttga aacaggcgag gtattcattt tgcaacacac
     5221 atggatccca caagctagat ttgatcgaga taacaatcaa gagcgtatca aagcgtggga
     5281 gaaggtggga gatctaacga ttattcctgg tgattacgtc aattatgaat acgtcttaaa
     5341 ttggtttgta gaaaattcga aaatctatga cattgtaaaa atcaattatg acaaggccaa
     5401 ggcgctacga ttaaataaag aactagaaaa tgcaggattt gaaaccaacg agattcggca
     5461 agggtttcta tcattaggtg ggccaatgca aaacttcaag gaaatgctat tggacggtaa
     5521 ggtgattttc aacaattcca agctttaccg atggtatcta tccaacgtca agctggtgat
     5581 ggatcgcaac tcaaactgga tgccgtctaa gcagtccaag agtagaaaaa tagatggttt
     5641 tgcagcaagt ttgaacagcc acgccgaagt gttgaatatg ttggttaatc ctgtcggaac
     5701 cgggaaagta acctattact cgatttccga tttaatgaat atgtaagaaa ggtgtggagg
     5761 aatgagtatt ttagatcgtt tgcgttcttt tggccgagcg aagccgaaag cgagcaaaca
     5821 agagtatttt ttgaatgacc cgggattgat accgtattta gtcggaaaag atgaaatatc
     5881 agaagggatt ttttccgtaa ttagccgtgt atcgaacgtt tttgcgtctc tccctctcaa
     5941 aatgatagat gtggagtttg gccaaccgga cgactgtcct gcatacaact tgttgagcga
     6001 aggccctcga tattttacaa agtttgattt tttccgggac gtggaagttt tgagaaacta
     6061 ccaagggaat gcgtatgtgc agattttccg aaatatcaat ggagaagtag cagatatggc
     6121 gttagtaaaa cctggtgctt gccatccagt gattgatatg gatagcgggg agctttacta
     6181 ccaagtaact gcgactgaca aaggcagtta caagcaagtt atctatgtac attacatgga
     6241 aatgcttcac tttaaacaac cgaggtttgg cggcttggaa ggtacagacc ccacaaaagt
     6301 attaacgaat accctcggat atgatcgaga agtccgaaaa atctctttaa gtcagcttaa
     6361 aggaagtaat gaagggctaa aagttaagtt tgctagcaat atggatgaag aagctaaaaa
     6421 agctacagtt aaaaacattg ctgattttta tcgacaaaac ggtggactac ttgtggaaga
     6481 aaacggtgta gaaatcgaac gtttacaacg agagctggta gacagcaagc ttttagatac
     6541 tgataaagta tctcgctcca gaatcgctat ggtctacaat gtgccggagc atttcatcgg
     6601 gaataaccag tcgagttact cctctcagga acagctcaat atggaatttt tgacgtacaa
     6661 tctagtaccg accgttaatc aatatgaagc ggaactaaat aagaaaacac tatcgagaac
     6721 cgaaaaagca aaaggttatc gatataagtt taatgtcgca agtttgctaa gagctgacac
     6781 tcaggcaagg ggacaattct atcaaatcat gcgacgaggc ggagcatact cagcgaatga
     6841 tgtccgaaga tttgaggact tgcaaccgat aaataaaacc ggtatggatg attaccatat
     6901 ttccggagac ctatatccaa tcgatatgga tccaacatta agaaaaacaa cctcgtctaa
     6961 aagcgtagcc gaaaacggtt aggttttttt agtttgcacc gaagggaggt ggaaaaatga
     7021 aaaaagtgac gttaagcggc gatgtcgtgg ataacgatac cgcgtggctt tatgactggt
     7081 ttgggatcaa ttgtatctca ccagggaaaa tttctgccgc tcttacagaa gcagcggggg
     7141 atgaagtaga acttgatatc tcatcgaacg gtggggatgt cctagcggca agcgaaatat
     7201 ataccgctat ccgtgcctat ccgggaaagg tatccggaaa tgttgtgagt attgcagcaa
     7261 gcgctgcaag tgtaatcgct tgcgcttgcg aaccgcttag aatctcacct acggcacaca
     7321 tcatgattca taacgcatgg gtgaccacta gtggcaacgc tgaggaatta aaagccaatg
     7381 cagaaatgtt aagcagtgtg gatgagtcta ttgttaatgc ttacgagatc aaaacaggac
     7441 tcgatcggaa aaaacttgct gatttaatgg cgaaagatac ttggttaaat gctcaaacag
     7501 cagtagcaga agggtttgca gatgaaatta tgtttgcaga agcaccagta acggtactca
     7561 atgcctctca accggttatt ccaaaaaacg cagtaactaa gttgaaaaat ttaatactca
     7621 aagcggaagc accgcaagaa gaaacactct tacagaaaaa actaaaagcc ttaaatggag
     7681 ggaaaaacga atgaatttag aacaattaaa aaatgcgtgg gtcgaggcgg gaagtaaagt
     7741 ctctgactta aatgcacaac tcaatgcagc attggttgac gatgaaaaaa cagaagaaga
     7801 tgtagtaagt ttgcaagcac aagtaaaagc agcacgggct aaacgggacg gattgaaaga
     7861 gcaagtggca aatatggaag ccgaacaagt cttaaacgtc aaaaaagaac cattagataa
     7921 aaaagatgaa aacttgaaaa acaagtttat caaagacttt aaagcgatgg tcaatggtga
     7981 tcctgctatt atggctactt tgacatctaa tacggatgaa tctggtaatg ctatcggatt
     8041 gactattcct gtagatgtgc aaacgactat tcatactttg gttcgtcggt ttgactcttt
     8101 gcaagaatac gtaaacgttg aaaaagtgac cactaccagc ggttctcgcg tttatgaaaa
     8161 atggtctgat attacaccgt tgactgcttt ggatactgaa gacggtgaaa tcccagcaaa
     8221 tgatgatcct gcactttact tgatcaaata cttgatcaaa cgctatgcag gtatttccac
     8281 agtaactaac agcttgctaa aagatactgc cgaaaacatt ttggcatggt tgtctaaatg
     8341 gatcgcgaaa aaagtagttg ttactcgcaa tacaaaaatc ttggcagcta ttgatggaat
     8401 caaagcggca caaaagaaag atgttacaga tgttgatgga attaaagata tcgtaaacgt
     8461 ccaacttgat ccagctatcg aagctacatc tatgtttatt acaaaccaag atggcttcaa
     8521 tgttttagat aaagtgaaac gtgctgatgg atcttacttg ttacaaaaag acgtagcttc
     8581 tgcaactgga tataccttct taggtaaacc aatcaagaaa attgcttctc gtttcttgcc
     8641 aaacaaaggt acgcaagcta ctcctaaata tccactgtac attggtgatc tgaaagaagc
     8701 cgttacattg tatgatcgcg aaaacatgag cttgctgaca acgaatattg gtggtggagc
     8761 ttttgaaaca gacaccacta aagtacgcgt catcgatcgc ttcgatgtgc aactagttga
     8821 tgatgaagcg gttgttttgg ctacttttac aactattgcg aacgagacac cggcgaaagt
     8881 ttaaggagct gattccttat gattcttgat cctaaaatgg atttaggcga aatcaaaaac
     8941 gcactaaaga ttgataccga tgatgacgat gtggaagtaa gccgtgcggc acaagctgca
     9001 attgcataca ttagaggggc tatcgggaat gataagccct ctttttataa gcaagaaaat
     9061 gaaacggttg atctgattaa tttagctatc ctgcaattag cagatcatta ttacaacgct
     9121 cgatcagcaa ccgtgagtgg gaacttgcga gagtacgatt taggttttac aagcctaatc
     9181 ttgcaactca aagcaagtta tttgcttttt gtggaggagg agtagcgtat gccccttatc
     9241 caaacaggaa atttaaatca acgcatcaag tttgtccgag atacgacggt taaggatgag
     9301 gacgggcaag ttgtcccgac ttctacaacc attcttactt gctgggcaag tgtgcagaca
     9361 caacgcctga acgatattaa gacgtcgatt ggtacggctt tggaaggaac actgacgttc
     9421 attatccgct accaacaaaa atcagagctg accaatgata tgaaagtgcg ttggaatgga
     9481 aaaacgtttg aaatcattac gattacgaaa ggcgagtttg cgaaggactt cacgacaatc
     9541 attgcaaaag aggtttcaaa atgagtgtag aagtcgatgc aaccgaagtg tacaaagcgc
     9601 ttagggaagt aaaagcaaac gttcaacgag tggaaagccc agcacttaga aaagctgggg
     9661 agtacgctca agaagagtta cgacaaaaca caccttactg ggatggaacg aagtcaaacg
     9721 gtaaacgtgg ttcgtatatg caagaacatg ctaagaacca tgtggttaca agctcgataa
     9781 aaaacggatt ggtagaagtc ggctatgaca aagatgtttc ttggcggatg cactttatcg
     9841 agttcggaac aatcaaacaa cgtccaaaag gtttcgtaca aaaaacacaa aagcaaatcg
     9901 aaaaacaagt aacacaaatc attgctgacg aagtaaaaag gaggctagga ctttgaaaac
     9961 ggcagtatca caagtctatt caattctgaa tagcaatgaa aaaacaaaga acattgattt
    10021 ttacaccaat agtgttccgg aatcagctca aacagtacct agccttccag ttggcagaat
    10081 tacagagata tccggcaact atgaagattt tgcaagcaac aatcctttga ccattcaatt
    10141 taacgtacag gtagatgtat gggtgtcaac catgaaagag gttgatgcct tttattttgc
    10201 ccttgatgag gttatgaggg ggaatggttg gcaatgcgca tacacggaac aaacagatga
    10261 cgaggacttg gaaggtgcaa agcggattat caaacgatat gtagcaaata tttcactaaa
    10321 ttaaaaggag agaaaataga tggcaacagt aggattcgag agcgtcattt ttggcgtaaa
    10381 aacaggtgca ggcggcactc taaaagaatt agtagcagat aagtcgaaag gcggagcgat
    10441 cgaagctaaa attactggat taggcgcaac ttctaacaca acatacgctt caaacgtacc
    10501 gttcttcatt gcaagtaaag gggtttcgtc gccaaaagtt acgcttgacg tggcagactt
    10561 aatggataac ggcatttaca gcgaaatcat tggtgctaaa accgtggatg gtgcaaatgt
    10621 aattggttca gaaactgaag cgccttacgt gtcggtagtc atggttacag cgaacaaaga
    10681 aggaaaacgc ttgttcatgg gattgacaaa aggaaaattc agtcatccag atatcgacat
    10741 gaaaacagct gaagacaaag gggtagaatt gcaaaccgat tccatcgaag gggaattcat
    10801 ttctgatgaa cgtggctatg tatacttaac agccgtagaa tcagaaggaa tgaccttaca
    10861 aaaattcaag gacttggtaa ataacaaaga gggggagtag ttaaccctgc atctacacca
    10921 acgacagata cagggacacc aaaagaacca gaaccaaaaa ttgatacaca aggttagcca
    10981 tttttggcta gccttatttt ttgtaaaaac aaggaggaaa acaaatgatt gaattgcaat
    11041 tgaaacttga cggaaagaaa aaaacattca aacaacaaga tatttccgca cgtgcaatgc
    11101 gtgagtgtat caaattttac gagaaagcgg aaaaagcaga cctaactgat ttagaagcga
    11161 ttgattcaat gattgcaatt acagcagata ttttccaaga tccagcagtt acatttgatg
    11221 ctattttaga cggtttgact gcgagcgagt tagtaccggc attagaaagt gtttttgaac
    11281 aaataaatga actgggaaac aatgaaaaaa agcagacggc gagcaaaaag aaataagttt
    11341 ttctgaagct aggaaagcaa tggatcaaat ctacaaagat ttaatcgaag caggttggac
    11401 gatgagagat gtggacgaag ccgactatca ttatttgtta cacctttttg gagaagtgga
    11461 gagtggcgaa gaatatgtag atggtgctga tttcatcaaa caatttttat cggctgaaga
    11521 cttagtaaaa cttgaggaag gaggtaaata atggcaggaa aaggacaacc ggcaggaaat
    11581 atcaagctag ggattagttt agatagcact agttttggta acacgctgga cgaaatcaat
    11641 gcgaaagtca aacaagctga gtcgaatatg cgtgccaatc taaaggctta tgattcagca
    11701 ggacgttcat acgaagcact tagtcaaaag acgaaagact tgtctacggt tatggaaggg
    11761 caaaacgcca aagtaagaga attaacaaag cgccgtgatg aagcgattag caagtatggc
    11821 gaggaatcga aacaagttgc taaccttaac acacagataa acaatgctac cgcaaaatat
    11881 aatgcttaca gtcgccagtt gaacgacaca aaaaaagaat tggtgtattc caaaacagcc
    11941 gtcaatgatt tatctaatga aatcaaagaa aatgaacgac aaatgaacgc cgaagtaaaa
    12001 gcgttgaaag ccgctggtga tgaatctggt gcttttgaag caaaacaaaa agggctagcc
    12061 aaacaaacgg aattatccga gaaagctatc gaagaacagc gcaaagttgt gaaactgatg
    12121 gctgatgagt ttggcgattc agcaaatgaa accgaagatg caaaaagggc attagaaaag
    12181 ttagaacgac aaagccaaat atctagcagg caattagaag cactcaaaag ctccagcgat
    12241 caatcaggaa aagaaataga agattttggc gacaagtcca caaggtcagc taggaaactg
    12301 gacggactaa aagacaaatt aagctcgcta aaaagcgcat tttcgtttgg tgcagttgct
    12361 ggattagcgc ataacgctat tagcagtgta gtaagtggcg tgcaaggttt ggttggcgaa
    12421 gcagtaaacg catcggattc attgatgaag ttttccaaaa ccatggagtt tgctaacttt
    12481 gggaagtcac agatagaaag ctcgaaaaaa gaaatgaaag actacgccga taagacggtt
    12541 tatggtttag aagaaattct gaacacaacc gcacaattgg catctaatgg gattcctaac
    12601 tatacagaac taaccaaggc ggcaggtaac ttgaatgccg ttgcaggcgg ttctagtgat
    12661 acattcaaat ccgttgccat gatgctaacg cagacggcag gagctgggaa actaacaact
    12721 gaaaactgga atcaattagc agatgcgata ccgggtgctt caggattgtt acaagacgct
    12781 atgttgaaaa acggagctta tacaggaaac ttccgtgatg caatggcgca aggtcaaatc
    12841 acttccgacg agttcaacca agcaattgta cagttaggta tgaatgacgg agcagttaag
    12901 gcagccactt ctacagacac attgagcggt tcttgggagc agatgaaatc cactgtaata
    12961 aatgggctac aaagtattat agaaaaaata ggcgttgaaa atatcactgg tttcatcaac
    13021 agagtaacaa aagggattga aaattctatt cctaaaatta ctcaatttat aggttggttg
    13081 agagatattg gaacgtggat cgttgaaaat agagagccac taacatggat tgtcggaatc
    13141 ataggcggaa ttacattagc agtaaaagca ttgaacgtag caagtatgtt gctggcaatt
    13201 actggcggaa cattggcagc cccttttgtg gcgattggtg tagcattagg cgcactagca
    13261 ggtgctttgg tgatagctta tacaaaatct gaaacattta gaaatatagt caatgcggct
    13321 tttacagctg tgaaaaacgt agttatgagc gttatcaata atttggtgga atactacaaa
    13381 atgttgtgga gcgtgttgca gtggctttgg gaaaaaataa aagaatgggc ttcatggatt
    13441 ggcaataaat tcattgaaat gaagaacagc gttgtgaata cggttcagaa cctatggaat
    13501 ggcgtgaaaa acttcttcag caatggcgtt ggagatacgt ggaataaggt agtcggttgg
    13561 gtaaaaaaca ttttcaacaa agcaactgaa ttgaagaaca acgtttctga tgtaatcggt
    13621 aacctgtgga atggtatcaa agacacattc cgtagaggta tcgatacggt attcaattgg
    13681 ttttcagaac taccaacgaa gatgaagaat gccattattg gcggtaaaaa cgccattgtt
    13741 gatgcgttca aaagtatttt caacgcagca cttaaagcga taggtaaacc agttaacgca
    13801 atcatccatg gagcttcatg ggtactagaa aaattgggcg cagaacctct acaagaatgg
    13861 gatgtaccac aatacgctac aggtacacca gcaggtgggc acccaatcaa tggtccaatg
    13921 atggttaatg atggacgtgg agcagaaaca gttatcacac cagatggtag agccttcatt
    13981 cctaaaggac gcaatgttgt tctaaacgca ccaaaaggaa cgcatgtctt gacagcagaa
    14041 gagaccgcgc aacttcaagg ttctaaagct cctaagtatc gttacaaaaa aggtactaac
    14101 ttctttggta acatgtggga tagtgtgaaa aaggttgctg gtaatgtagg tcacacaatt
    14161 aaaaacgtag taggtgacgt gtgggacttt atttcagacc cgggagcgtt agctagaaaa
    14221 gtacttgggg ggttagatgt attaggtgga ttgacaaagt atccattaga agtaggtaaa
    14281 ggcatcctat ctaaagcaac aagtgcactg actgaaaaaa tcactgggtt gttctcatct
    14341 ggtaacttag atacctccgt aggaacaaat ggcgtctata aatatttggc tgatgttgct
    14401 aagtctgtga tgaagaaatt cccaggattt gtggcaacta gtgggtatag accaggtgac
    14461 ccttattcac atggtaaacg taatgccatt gatattgcat taccaggcgt cacaggaggc
    14521 tcacctagat acacagaagc agcaaactac gcttttgaca aattcgcttc caaaattggt
    14581 tacgtaatca ctaatgggaa agttcgtgac cgttcaggac aatcaggtca accagcaact
    14641 ggtgcatggg agccatggcc cgatggagat cactatgatc atgtgcattt aaacggtgtg
    14701 aaagacccac aaaacactca aatttcagga gatagcgtgg gaggcagtgg ggtagaaaga
    14761 tggcgcaatg tagcaattag agcgttgaaa atgaccggtc aatacagtac tgcaaactta
    14821 aatgcattac taaatcaaat gcgtacagag tcaaatggta atcctaatgc agttaacaat
    14881 tgggatatta acgccaaaaa tggaacacca tcaaaagggt tgctccaagt gattgaccca
    14941 acattcagac agtatgcaat gccaggattc aacagcaata tttttgaccc actatctaac
    15001 atcttagctt caatcagata cgcactatca agatatggct cactaacaaa tgcctatcgt
    15061 ggagttggtt acgcaaacgg tggaattgta aaccaacatc aaattgcgga aatcgcagaa
    15121 ggaaacaagc cagaaattat tattccgtta gataaggcta aacgatcaag agcgatgcaa
    15181 ttattgtcta tcgcaatgga caaactagga gtttctccta aaacttatgg aaatgcgaca
    15241 actgtttcca gtgattacga aatcctagaa gccattgaaa aacaggcggc aacaacgaat
    15301 cagctgttat cgttattgct ggcatttttc aaaggaaata gccgaactga tagagattta
    15361 gctttagata ttcagaagat cttggttagg aggatgtaga tgcgaactgt attactgaga
    15421 aatagaataa acgaagaaat tgatttgtcc acagaagact attttgctac tgggttgagt
    15481 aatatggggt ttgaagtaaa aaaagaacat gtgggacagt gggggaattt cagagaaagc
    15541 agtgagagcg ttgaaatatc tgaatttcag tcatctgtaa tcatttctgt gcatgggttt
    15601 cgagaaaaag aactgtacaa ttcacttgtg cagttcctat cggaaggtcc gtttgaactg
    15661 gaatttgctt ttgacggcga aacaatggta agaagatgca gtctaaaatc tttaagtaag
    15721 actgaaatcg atccgaaaac atcgttacta acagacactt tagaactata tttcacatca
    15781 aactggtatt ccgtaaaacg cgaaaaatta attcagcgac cgaatgtagt gaaaacacgt
    15841 ggtaaggttt ttccgtataa aagatcttat atctacactc aaaacttgtg ggagaaaaaa
    15901 ggtgtattca aattcaataa taattctgta tacctaacga atagcaagga acggatgtcc
    15961 ccgctaaaaa ttcgtgtgat tgggaagtgt tcaaatccgt attgggaagt aatccaaaac
    16021 tcacaaatca tcgcaacgga tggatacttc atagatatga ctgaaacaca gactctagaa
    16081 gtatcaagcc tttttgaaga tacaacggcg attttaaaag atattgcagg ggtagaatct
    16141 tctgtctacc aacaacagga ttatacaaaa actaattttg ttcaggcccc aactggagaa
    16201 ttcagcattg tgtttcatgt tgggggggca gacgtagaga ttgagttata cgaggagcgt
    16261 gatctgtttt gattttagca gtcacgcttt ttcatcgtga tttaatgctt tataacgaac
    16321 atttattctc taacagtttt gaatttggtg tggacgagat caacgaagag gctagtagct
    16381 ttacgatgga taagtacgtt cctgtaaaaa caggcgattt tctactagca aaatatattc
    16441 ctagtggaaa atttgcatat tttggtgtaa ttacgtcgca ggaagacgaa aaaatcagtt
    16501 gtaaaagctt acttagttta gctgatagtg agataccgac tgcacgtgtg tcaggagata
    16561 actacgaaga gcatatccgg cggttgattg aatactattt gctgaatgat ccaacgaaac
    16621 aactaaaaga tattttagac gtcaaagcag aaagtgcgac ctctcattcg tatcaagcta
    16681 ccgatacgaa taaacacaag ttaagtgcgt atattctcaa tggtttcaaa aaatacaacg
    16741 taaaatggta tttcaaggga attcaaaaca gaaaaattta tacaggcata cgtgctgtaa
    16801 atgaatcaat ttacattaaa gacaattctt ctgaatttag cgattgggat gtgtttgttc
    16861 aggcgccggg cgctggaaac gagaacaagc tattaatcgt tgataaagca atgaaagata
    16921 tagagaaacc gataatactg tcaacatggt atttggacga agaaaacaat ttgacacaag
    16981 atggatcgaa agaaaatatc acgaaaccaa ctgtcaattt ggttaatatc tacgatcaaa
    17041 ccgcagaaga taaagcatcc tacgaagatg tggcaaaatc agagttgaaa ggcaatacgt
    17101 attcgcatga aatcaaagtg aacgttgtaa gaaatgcaaa aaatttgaat gtcgaaacga
    17161 ttgaaacagg gatgtttgcc acgatttctt ataaaggaaa gatatacaag tcggttttga
    17221 cagcttggcg aatatcaagt gataaggagt ttgtggaatt gactttcgga aacataagaa
    17281 gtcgttttat ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg
    17341 gatatcaatt tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt
    17401 tatataatcg gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa
    17461 gcggattaac agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg
    17521 ttcgacaaga agaaagtata acagttccag caaactcaag tggttatata gcattaacag
    17581 tggatttgac acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat
    17641 ggactaataa tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca
    17701 atggtgataa ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt
    17761 caatctcaaa gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga
    17821 gggggactgc gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga
    17881 cagtttcagg ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata
    17941 gatatgtaac tacgcctttt tataaggagc gtgtaacttt tactaatgtt ttaggagaaa
    18001 ttgtttcagg atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt
    18061 cgaatactaa tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg
    18121 tgcttaggta tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat
    18181 taaattatct gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg
    18241 tgatgggggg ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt
    18301 tgtaattaat ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac
    18361 cgagggagaa ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa
    18421 gtcaaatttc agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa
    18481 cggcaaaaaa gtaacttttc agaacttcat catagacgtg ttagaacgag tggatattaa
    18541 ttcggagcaa gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac
    18601 cactttcaat cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata
    18661 taacgaatta gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgatca
    18721 aaccaatcag caaatcggcg atctcggcaa gctgaaaaag atgtacagta acagcatcga
    18781 ctttgggggc tatgattatt cggggaatcc gaatttgtta agtaagctat catacgactt
    18841 aattgaaaat caaaatactt cagctggaac actttctaag ggtgaaaact cgtttaaata
    18901 taataagata tcagctgaag tggaaggtgg agtagagtta tattataaac gaagaggtat
    18961 agctaactgg ttaccctcta ataaaacgct tgtaatgact gttaagctta gagctggagc
    19021 ggactatagt ccagttgacg gaaaacttat actgattaga tataggtatg ttgacagtgg
    19081 aactggcaag attgttttag acttacctat taacagtaat tcgataactc aggaatggaa
    19141 agagtttagt attactggaa ctactccaac atttagccca caagcatacc atccttggat
    19201 acaatttagg gctcaagatg gggtacttgg ggaaatagaa atgagctatg acatcaaaat
    19261 cgaagaaggc tctacagcta caccatatca gcctaactta cttgatgact cttactggct
    19321 aggtaaaaca ccgttgggtg agaatttgat aacgaatgcc aagttcccaa ttataacacg
    19381 aagcaatcct atttctggtt ttgatatttc agaagaatta ataataggtg aaacttatac
    19441 ggtatcttta aaagggacta agccagctac taaagaattc cacttatact acggagaagc
    19501 aaattaccag cagttgcagt atcaggcaac tttacttcct gtggaaggat taacagatgt
    19561 gtggagtgcc acttttacag ccagaaatac tagtgaaacg acaaatttac ttagagtagc
    19621 gttatggcaa aaacctaatt ctgcaacata tgatactgtt caaattgact ggctcaagat
    19681 tgaaaaaggc gacacacgca caccaaatat cagtcagttt aaatactttg gtgaaggctt
    19741 gaaagacagt aacaatccga acgactacag ctgggatgtc acaccagagt atactgaaaa
    19801 aagcttgaat aatacggtta gtctgactga accgcaatcc atcgaaggat tgaaaaattt
    19861 cgaagatggt attcaatcaa aaggaaaatc tgtattgaca tcagacgaca acaaatatga
    19921 agtcgtaacc ttaacagtta caaacgggaa taccggatca gcaaagcttt atcgtgaagg
    19981 aaaaaccgtc actatttatt tttttgcgtt gaatgggaaa agcagtggtg gaaatgattc
    20041 aacgatacta acaattccag aaggctatcg gccaccaatt agttttgagc aactggttgg
    20101 ctcgatagac cgttctactt tgaacagtgc tcagttatct attggtgcag atggagccat
    20161 taaatggcga agaaactcaa gttatggatc ggattatacc tttgcaatta cttacacgat
    20221 ttagaaaggc gtgaatcgat atgaaggcag catatagacc aattgaacct tacggattcg
    20281 agcaaatcat tgtgaatgat gaagaacatt taccggaaga atgcacagaa gtcgaaccac
    20341 cgattccaaa ttggaaaccg aaattcaatt actgggaggg aaataaatga aaaacatttg
    20401 gaaatatggc cgtactggcg gagagtatgc aggaaaagta ttggacgaca tgcttgtatc
    20461 cgttccttac acagatcagc caccgctcga aggggttcgt tcagatggcg aaccgctaac
    20521 gattgctgat cagatgtttg atcctaaatt gaaccaatgg attattttag cgaacgcgtt
    20581 agatcacaac gatttaaaca atctcaaagc gatgtacgag gctcttgaac atgaaaacgg
    20641 caacctaaaa cagctaaatg ccaaactcat gctaagcgat gtagcgatta aacaggaaaa
    20701 tactgcattg aaagaaaaag ctgacagttt agcacaaatc aattcaaaaa tgatgcttgc
    20761 ttcgttacaa aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa
    20821 gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    20881 ttactgacga tcaagttcgt ctattcgttc cactatgtat cacagatgaa gaagcagata
    20941 gaattattag caaagaagag agcgcatctt aagtgatgcg tttttttgtt ggaaagttgg
    21001 tggaacatga aagaagaagc gctccaagac gttgtggaga gattagtaag aattgaaaca
    21061 aaattagaca actacgaatc acttagagaa aaagctgata gtgcaaaaga tttggcagac
    21121 aaagcctact cagtagcgct aaacaacgca gaagatatca aagaaatgaa agccaataat
    21181 aaatggtcgt ggggttacat gatcggttta ggcattacga tcattggcta tttcttgact
    21241 aaattgtaaa ggagttaaga agaaatgatt ttacccgata agtattatca agtcattaaa
    21301 tggacggttt taacagtatt accagctgca tctgttttag tagccacgtt aggaaaagcg
    21361 tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc aacattttta
    21421 ggcgttatca ctggtgtgtc tgcatataac ttaaaaaaat aggaggaaaa aaatgaagaa
    21481 gaaaatcatt ttatcattga gcctaataat ggctcttttt ttattgccaa ttaacgggtt
    21541 cgcctatacg atcaacaatg aatttaattt aggtgtaaat gaaggtagct cacaagtagc
    21601 aaataaccaa tacatcctat tgcatgaaac agcaaacgaa actgcgacag gacgaaatga
    21661 agcgcagtac atgaaacgtt cttggtacaa tgcctataca gcgtatattg ttggcgatgg
    21721 tgggatcgtt taccaagttg gacaacctgg ttatgtacaa tatggcgctg gttcatatgc
    21781 taatgcaaat agtcctgtgc aaatcgaact gcaacatacg cacgataaag taacgtttga
    21841 aaagaactac aaggcatatg ttgaattagc gagagattcc gctaataaat acggtattcc
    21901 acttacatta gacacaccgt ataatcaacc aggaatcaaa tcacatttat gggtaactca
    21961 aaacatttgg ggcgatcaca ctgatccgta tggatactta tcagaaatgg gtgtaagtaa
    22021 ggaaaaactg gcttacgatt tagctcatgg atttacagac gaaaacccaa cgacttctga
    22081 gaacaagcct gtcattgatc caacccgagc aggtgcagca aatccaactt tatcagatgg
    22141 aacgaaccat tctcacattg atcagtttgg agaaatcgaa aatgcgaact tacacgtcgc
    22201 tggatggcac atcgctaact ataaatacga gtatattttc attatggact acaatactgg
    22261 gaaagaatta gctcgagtaa gagctgatgg aatttataga ccggatgtaa atcaagctta
    22321 taatacttct ggaaacgttg gctatcatgt atcttttaac atgcgcaatt tccctaataa
    22381 gaaagtatac gtcatgatgc gggcaacgaa tgatccagaa ggaaacacta aaggcggtgc
    22441 gcaagatttc catgacaaac gttggtattt aaatattcct aaacgataaa aatagctcct
    22501 cgttgaggag cagtacatag aattgaaaat taacgttaaa tcaaaaaaat atttacaaga
    22561 tgtttattta agtagataat aaaagaaaaa agcaggtgaa attgtgacag cagaaattgg
    22621 tattatgaat aaaagtggaa tcgtgttagc ttcagattca gcatctacga ttggagatag
    22681 caaagtatat aatactgcaa aaaaattatt tactttggat tccatgcatt ctgtaggtat
    22741 catgatatat ggtaatgctg aatttaatgg tattccttgg gaaataatca ttactcaata
    22801 taagaaaagc attggtagtt ctgtttttaa tactttggaa gaatacgcgg ataattttat
    22861 agaatttgtc aaaacagcat cttttattag aagtgaacag accgagcaag aacaaatgat
    22921 tggggttttt caaaaaataa tttcaggttt atttgagagt atagaagtag atattaactt
    22981 tttaataagc caaggtacac aaatagacaa agatgtccta gtgaagttat tacaaacaaa
    23041 aatgaatacc aatttgtctc aacagtccca gacattcatt ttagatattg aaaaggcgtt
    23101 atttcttagt aactatggag aaatattaaa agatattctt aatagtattt ctacaatgga
    23161 aggtgtttca gaagctatat cagaggaaat tcaaagttac gtttatgaaa ttataattag
    23221 agacgatgtt tattcttccc ctacaggaat agttatagca ggatatggaa gaatggatat
    23281 tttccctaaa ctatattctt ataatatgtt tggatttgtt atgaatattc tgaaatactc
    23341 agaatatgaa agtgctcaaa taggaaatga caatggttca ttaagatcta ctatattacc
    23401 atttgctcag tctgatgtgg taaatactgt agttcaaggg gtagatccac aaattactaa
    23461 ttatctgtcg agtcaagtcg atagttttga cgataacgga aaaaatacct atattaatat
    23521 tataaagaat atttcagagt ttcaacaaaa tcaattcata tttccgttac taaacatgat
    23581 tgctttactc ccagttgaag aaacagcaat tatcgcagag actttattaa atttaaccag
    23641 ttttaaacga aaatatacta cttctgtaga aacagtaggt ggcccaattg atgttttagc
    23701 tattacacct aatgatggcc ctatttggat taaaagaaaa cattattttg atattgataa
    23761 taatattgga tacagattgc gaaaggagag agcgaatgat tacaataatt aaacctcagg
    23821 aaaaatcccc tgttttgaaa tttaatgaga gcaatctagc ccataataaa atgagtataa
    23881 ctgatacagt atttgatgta atggttgagc aacaaaaaaa aattaataat aacaatagac
    23941 aaagaaaaga aattaaccaa tattaatgtt ttttctgttg cgttttttgc agtcataatt
    24001 taaaacctcg ctcaatcttg ggcgaggtct ttttttattt ttctgaaaag ttaaagtaaa
    24061 taatagcata aaataatata ttttacaaag aataagtaca atctagtttt ttgctattaa
    24121 atgtgtaata ataaatatgc catcacaaca tgaagaatga aatccattat tatctagtct
    24181 agtccattct ttttgtttgc agtatttgtg atggctttc
//
LOCUS       DAZKOM010000031        24038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000031 DAZKOM010000000
VERSION     DAZKOM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.031"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..790)
                     /locus_tag="ACG8NF_001253"
     CDS             complement(56..790)
                     /locus_tag="ACG8NF_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0471742.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="ACG8NF_001254"
     CDS             1117..1527
                     /locus_tag="ACG8NF_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0471743.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="ACG8NF_001255"
     CDS             1520..2221
                     /locus_tag="ACG8NF_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0471744.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="ACG8NF_001256"
     CDS             2221..3807
                     /locus_tag="ACG8NF_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471745.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="ACG8NF_001257"
     CDS             3826..4047
                     /locus_tag="ACG8NF_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0471746.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="ACG8NF_001258"
     CDS             4044..4805
                     /locus_tag="ACG8NF_001258"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0471747.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="ACG8NF_001259"
     CDS             5185..5694
                     /locus_tag="ACG8NF_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0471748.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="ACG8NF_001260"
     CDS             complement(5769..6308)
                     /locus_tag="ACG8NF_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0471749.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACG8NF_001261"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACG8NF_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0471750.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="ACG8NF_001262"
     CDS             7321..8172
                     /locus_tag="ACG8NF_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0471751.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="ACG8NF_001263"
     CDS             8200..8835
                     /locus_tag="ACG8NF_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0471752.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="ACG8NF_001264"
     CDS             8855..9628
                     /locus_tag="ACG8NF_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0471753.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="ACG8NF_001265"
     CDS             10036..10833
                     /locus_tag="ACG8NF_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0471754.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="ACG8NF_001266"
     CDS             10811..11224
                     /locus_tag="ACG8NF_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0471755.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="ACG8NF_001267"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="ACG8NF_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0471756.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="ACG8NF_001268"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="ACG8NF_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0471757.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="ACG8NF_001269"
     CDS             16001..16561
                     /locus_tag="ACG8NF_001269"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0471758.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACG8NF_001270"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACG8NF_001270"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0471759.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="ACG8NF_001271"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="ACG8NF_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0471760.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="ACG8NF_001272"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="ACG8NF_001272"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0471761.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="ACG8NF_001273"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="ACG8NF_001273"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0471762.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="ACG8NF_001274"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="ACG8NF_001274"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0471763.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="ACG8NF_001275"
     CDS             21908..22486
                     /locus_tag="ACG8NF_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0471764.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="ACG8NF_001276"
     CDS             22538..23020
                     /locus_tag="ACG8NF_001276"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0471765.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="ACG8NF_001277"
     CDS             23137..23511
                     /locus_tag="ACG8NF_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471766.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23886..>24038)
                     /locus_tag="ACG8NF_001278"
     CDS             complement(23886..>24038)
                     /locus_tag="ACG8NF_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0471767.1"
                     /translation="YSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTL
                     VSMFTE"
BASE COUNT         7844 a         4317 c         5153 g         6724 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttgggagcg tcaataattt tgtgtaaata aattgtcctc ctgcaaaata
    23881 attagttact cagtaaacat tgaaactaat gtatcggtta cctgttgaaa acctttatgg
    23941 cttctgttta gaaatttttg attgtatgta tcaaaaatgc tgactagaaa gcgttctagt
    24001 gattcttcat tttgaaactg ctcttttcta cggctgta
//
LOCUS       DAZKOM010000032        24031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000032 DAZKOM010000000
VERSION     DAZKOM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.032"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..679
                     /locus_tag="ACG8NF_001279"
     CDS             110..679
                     /locus_tag="ACG8NF_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0471768.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(857..1297)
                     /locus_tag="ACG8NF_001280"
     CDS             complement(857..1297)
                     /locus_tag="ACG8NF_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0471769.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1455..2219
                     /gene="map"
                     /locus_tag="ACG8NF_001281"
     CDS             1455..2219
                     /gene="map"
                     /locus_tag="ACG8NF_001281"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0471770.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2251..3174
                     /locus_tag="ACG8NF_001282"
     CDS             2251..3174
                     /locus_tag="ACG8NF_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0471771.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3250..4389
                     /locus_tag="ACG8NF_001283"
     CDS             3250..4389
                     /locus_tag="ACG8NF_001283"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0471772.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4382..5182
                     /locus_tag="ACG8NF_001284"
     CDS             4382..5182
                     /locus_tag="ACG8NF_001284"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0471773.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5182..6009
                     /locus_tag="ACG8NF_001285"
     CDS             5182..6009
                     /locus_tag="ACG8NF_001285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0471774.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            6014..6721
                     /locus_tag="ACG8NF_001286"
     CDS             6014..6721
                     /locus_tag="ACG8NF_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0471775.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            6821..7687
                     /gene="rfbA"
                     /locus_tag="ACG8NF_001287"
     CDS             6821..7687
                     /gene="rfbA"
                     /locus_tag="ACG8NF_001287"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0471776.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7701..8273
                     /gene="rfbC"
                     /locus_tag="ACG8NF_001288"
     CDS             7701..8273
                     /gene="rfbC"
                     /locus_tag="ACG8NF_001288"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0471777.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8295..9323
                     /gene="rfbB"
                     /locus_tag="ACG8NF_001289"
     CDS             8295..9323
                     /gene="rfbB"
                     /locus_tag="ACG8NF_001289"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0471778.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9421..10272
                     /gene="rfbD"
                     /locus_tag="ACG8NF_001290"
     CDS             9421..10272
                     /gene="rfbD"
                     /locus_tag="ACG8NF_001290"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0471779.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10306..12339
                     /locus_tag="ACG8NF_001291"
     CDS             10306..12339
                     /locus_tag="ACG8NF_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471780.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12383..13663
                     /locus_tag="ACG8NF_001292"
     CDS             12383..13663
                     /locus_tag="ACG8NF_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0471781.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13873..14679
                     /locus_tag="ACG8NF_001293"
     CDS             13873..14679
                     /locus_tag="ACG8NF_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471782.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14691..15911
                     /locus_tag="ACG8NF_001294"
     CDS             14691..15911
                     /locus_tag="ACG8NF_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471783.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15901..17487
                     /locus_tag="ACG8NF_001295"
     CDS             15901..17487
                     /locus_tag="ACG8NF_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0471784.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17526..19664
                     /locus_tag="ACG8NF_001296"
     CDS             17526..19664
                     /locus_tag="ACG8NF_001296"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0471785.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(20032..21015)
                     /locus_tag="ACG8NF_001297"
     CDS             complement(20032..21015)
                     /locus_tag="ACG8NF_001297"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0471786.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21393..22784
                     /locus_tag="ACG8NF_001298"
     CDS             21393..22784
                     /locus_tag="ACG8NF_001298"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0471787.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22800..23840
                     /locus_tag="ACG8NF_001299"
     CDS             22800..23840
                     /locus_tag="ACG8NF_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0471788.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23859..>24031
                     /locus_tag="ACG8NF_001300"
     CDS             23859..>24031
                     /locus_tag="ACG8NF_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0471789.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGR"
BASE COUNT         7698 a         3998 c         4933 g         7402 t
ORIGIN      
        1 cagcttgtga agaaaatcaa aaactaattt tgcatatcaa aaaaaacatg ttacaatgaa
       61 attaatcaat ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa
      121 aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg
      181 atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat
      241 ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga
      301 tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc
      361 ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga
      421 agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt
      481 gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg
      541 gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac
      601 tgatgaacag attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga
      661 accaatcgaa atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat
      721 gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga
      781 actcttccat gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc
      841 ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt
      901 cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc
      961 ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag
     1021 tatcaccaga tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt
     1081 cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta
     1141 cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga
     1201 cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc
     1261 ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt
     1321 atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac ctagagacac
     1381 ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa
     1441 aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat
     1501 ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca
     1561 caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg
     1621 cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat tagtataaat gatgaaattt
     1681 gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt
     1741 gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta
     1801 cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc
     1861 aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag
     1921 gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg
     1981 aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca
     2041 tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta
     2101 acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg
     2161 ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc acttattgat
     2221 ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat
     2281 gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt
     2341 acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct
     2401 tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga
     2461 gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca
     2521 caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa
     2581 agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat
     2641 tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt
     2701 ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta
     2761 caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat cacattggta
     2821 gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg
     2881 aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg
     2941 tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt
     3001 tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc
     3061 atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca
     3121 agccgaaaat ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa
     3181 gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta
     3241 ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta
     3301 tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa
     3361 ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt
     3421 atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt
     3481 gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat
     3541 gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt
     3601 gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc
     3661 tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg
     3721 attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga
     3781 atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt
     3841 cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta
     3901 ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg
     3961 aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca
     4021 gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat
     4081 aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg
     4141 acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga
     4201 agaatcggaa cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa
     4261 ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg
     4321 aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata
     4381 gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa
     4441 aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat
     4501 ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa
     4561 atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa
     4621 tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt
     4681 ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat
     4741 gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct
     4801 ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga
     4861 caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg
     4921 gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt
     4981 tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc
     5041 aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc
     5101 gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa
     5161 gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag
     5221 caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa
     5281 attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa
     5341 gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt
     5401 gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg
     5461 ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct
     5521 gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat
     5581 atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca
     5641 atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct
     5701 gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa
     5761 actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa
     5821 gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat
     5881 gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc taaacagttc
     5941 ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg
     6001 aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag
     6061 agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga
     6121 tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg
     6181 aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca
     6241 gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa
     6301 agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag tagtttctga
     6361 tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa
     6421 ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc
     6481 tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga
     6541 tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct
     6601 gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa
     6661 aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg
     6721 ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga tgagataata
     6781 acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc
     6841 tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc
     6901 gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa
     6961 agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa
     7021 cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc
     7081 acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg
     7141 ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga
     7201 tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga
     7261 atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa
     7321 ttacgctgta actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat
     7381 caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa
     7441 aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca
     7501 tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa
     7561 agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct
     7621 ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc
     7681 tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg
     7741 tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa
     7801 aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg
     7861 aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga
     7921 ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga
     7981 cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac
     8041 ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt
     8101 gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag
     8161 cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc
     8221 cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa
     8281 ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta
     8341 atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac
     8401 tgacttatgc aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg
     8461 tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag
     8521 ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg
     8581 tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac
     8641 gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc
     8701 caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa
     8761 gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt
     8821 ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg
     8881 aaaaatttat tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg
     8941 gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc
     9001 tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag
     9061 ataataagac cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg
     9121 accatgtaaa cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac
     9181 gggaagagtt aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga
     9241 tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag
     9301 catatgccaa aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca
     9361 tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat
     9421 atgtttttat taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat
     9481 gaaaaaaatc tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag
     9541 aaaacaatgg cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac
     9601 acagcagtag ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat
     9661 ggtacaagaa atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt
     9721 acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg
     9781 aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt
     9841 ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc
     9901 gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag
     9961 tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa
    10021 aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc
    10081 gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt
    10141 ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta
    10201 ggatttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa
    10261 atgaataagt aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca
    10321 agaagtgcac gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt
    10381 gacctgtttc taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact
    10441 tttaatttaa acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat
    10501 gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg
    10561 gtgcagacac ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat
    10621 tattcattaa acggacaaaa catgatcatt gcgtataatt ctcctgtaaa ggatattacg
    10681 gttatcggaa aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc
    10741 ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata
    10801 ttaacgaaga gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca
    10861 gcagtccagt ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg
    10921 atgactgctt tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg
    10981 atgcttttgg tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa
    11041 gtgatgctgg cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag
    11101 aaagtttcat gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc
    11161 ggagtcatgg ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc
    11221 ttatatccag gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct
    11281 ttccttacaa attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg
    11341 gccctgttct atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc
    11401 aaaaaagata gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct
    11461 atcttttgga ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat
    11521 gttcctccat taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg
    11581 tttatcgctt atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg
    11641 gcagtaaatg catggtctta tttctatgcg attacgcatt cgaaaatgga taattatttt
    11701 tcggattttg aaaccatcgc agttgctgtg ttgggaacag tgattttagc ttttgcatta
    11761 tttggctgga gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt
    11821 ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa
    11881 atccaaaaaa tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat
    11941 aacttcaccc caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata
    12001 gctgcatgga agaaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct
    12061 catacaagag cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg
    12121 ttttcggtca cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt
    12181 aaacgaccat taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga
    12241 ccggataaag acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca
    12301 caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc
    12361 tgaaatgtta gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa
    12421 caaaatgagt gtcggtttct tgcttttgtt actgacgcta gtcttttttg tttggacagc
    12481 agggttaggt tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt
    12541 tagtgtggct attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta
    12601 tctaggtgtt gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga
    12661 gtctttattt ggagacttac tgtttccttt gattatttta ttttgtttag taattaaatg
    12721 gatcgagtgg acaaaatggg atcgcggtat ttatctgatt gtcttttcgg tcttcctcgg
    12781 aatcacgtta tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc
    12841 tccaggcaat tcgctttctg atatttggat caatacgaat ataatcggaa gttctttgat
    12901 gactcttgct ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat
    12961 catcggtgca gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg
    13021 tgcattagtc gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg
    13081 tgtcattcgt gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc
    13141 ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca
    13201 taaattctat cagacgctag gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt
    13261 tatcttccaa cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa
    13321 tatctatgga ttgataggat ttgggattgc tgctttgctg cttatccttt ttattggtcg
    13381 cttaacgttg aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc
    13441 ggtgatgatg cagtcattta tggaggatac attgacttca tacccatggg ttccgattat
    13501 ttatatcctg ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt
    13561 ctatgttatg gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca
    13621 accagatagc gctcattctt ccaggatcca acgctatcat tgataggcac aaaatacgag
    13681 agaggctgtg acaaccgtcg cagccttttt tggtttattg caattgtcta gcatcttctg
    13741 taaaatagaa acgttagaga aaatcttgta tcatagaaaa ttaagaaaga aataagaacg
    13801 cagctcggta ccaaaaatat ggtaaaatgg cacttgactg taattcactg aaaatcgctt
    13861 ggaggaacac aaatgtttaa agatttgtat ttatttatga aggacattta tcagaacaga
    13921 aaattattgc tacagttttc gatcaatgac ttcaaatcaa gatatgcagg atcgttttta
    13981 ggaatcttat gggcgtttat caatccgcta tttactgtat tgatttattg gctggtattt
    14041 ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc catttatcgt atatttagtt
    14101 accgggatga ttccctggtt cttcttctca gatgcatttg catcaactac ggttgttttt
    14161 cgggaatata cttatttagt aaagaaagtc gtattcaata ttcgtatctt gcccacgacg
    14221 aaaatcctat ccaatctgta cacccactta ttcttcattc tgattggcat agctgtcgcg
    14281 cttataaatg gtatatatcc ttcagcaatg tctttacagc tgatttatta tttattttgc
    14341 atgtgtgctt tcttgacagg attgacttgg ctgacggcat cgatccaacc atttttacct
    14401 gatatcatgc aattaattac gatcgtgctg cagttgatca tgtggacatt accgatatta
    14461 tggagcccta atcagtttga ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac
    14521 tatgttgttc aaggttatcg tgagtcattt ttaagtgaag cttggttctg ggagcatgga
    14581 acatatactt tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg
    14641 ttccgtagat tgaaaccaca tttttcagat gtgctgtaag gagaatagct atgacagaat
    14701 atgcaattag actaaaagat atcacaaaat cttataatat gtacgcaaaa ccttcggatc
    14761 gatttcgtga agccctcaat ccttttaaga aatcctatca tgatgtattt tatgcgttga
    14821 aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat
    14881 cgggaaaatc tacaatgttg aaaatcatta cgggagtact gacaccaaca tccggcacga
    14941 tggaaatcga aggaaaaatc tcagctttgc tagaactagg atcaggattc aatcctgagt
    15001 attctggcta tgaaaatatc tatttaaatg gaatggttct aggattttca agagaagaag
    15061 tcgatgaaat ggtcgatgat atcattgaat ttgctgacat cggagaccat atttatcaac
    15121 ctgtaaaaac ttattcaagc gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg
    15181 tgaatcccga tattttgatt gtggatgaag cattggcagt aggcgatctt gaatttcaac
    15241 taaaatgtat ggaaaaattt acagaaatca agaactcggg aaagacgatt ttattcgttt
    15301 ctcatgatat cagttcgatt cgtcgttttt gtgatcgttc ctattggttg aaaaacggag
    15361 aagtcgtcga atatggcgat acgatggaag taacgacgaa ttatgaaaac tttttaaaga
    15421 aaaaatcagt taaaacagtg gatcggaata aaaaccaagt agaacggttc gccgctccag
    15481 atatcgtcga cgtgacgaaa gcggaattgt tggataaaga caaaaatccg gtggatatgc
    15541 tggaacaagg agaaaaacta tacgttaaag taacttatga agtaaaagat gatacaatca
    15601 aaaatccggt tttaggaatt gcgctacgaa cagtcgacaa tttttatgta tgcggactta
    15661 atactctact agatggtatc aagattcctt ggaaaaaagg agagaatatc ttttatctgg
    15721 agtatgaaaa gttaggtttg ttagctggtg aatattattt tgacgttgcg gtctttgaag
    15781 aaaatgcaac ggttcctttg gtatataaga caaagtacat gaacttgttt gtcagcggtt
    15841 cttatatcgg agaaggcatc gtggtcttag accataaatg ggaagaaggg actcatagca
    15901 atgaaatatg attttgaaat ggacctagat gaacaaagca gtgtgggaaa aattgccgca
    15961 cagatcaaac caggaagtaa agttttagag tttggtcccg gaaacggccg attaacgaaa
    16021 catttaatcg gagcaaaaca atgtgaagtg agcattgttg agttagataa ggaattgttc
    16081 gattttgtca gcgaatttgc tcaagatgga ttttatggtg atatcgaaag ttttgagtgg
    16141 gcaaactatt atgctggaca gacatttgat tacgtcttgt ttgcggatgt tttagaacat
    16201 ttggtagatc ctggaaaaac attgaagaaa gtcagagaat ttttgaatga agaaggagag
    16261 atcctgatta cattccctaa cttggcgcat aattctgtca tgatcgattt gttcaataac
    16321 caattgccgt gggcttctta tggtctccta gatgaaaccc ataattcttt ttatactcat
    16381 gatggcttcc aaaaagtttt tgaaaaagcg ggattattca ttaatattga agattatttg
    16441 tacttagctg taggtgatac agaattaaaa tcaacttatg aagaattgcc ggaagctgta
    16501 cgctatgatt tcaaaatgcg accatttggt gaagtctacc aatacttttt ctcattgatg
    16561 aaacatccag tagcacaaag ttccattgca gagccacaaa attctaatta tgtaaaagtg
    16621 ttagaagtaa cccagcaaac gaaacaagat gaaacaatcc aacaaatacc attcaataat
    16681 ttcacagggg aaaatgagac actaagtttc cccgtatcag aaacaacaga aagaatggtg
    16741 tttcgcttcg cgcaacaacc tagctttatc gaattcagcg cagaagctgc cggagaaaaa
    16801 ctaacattta tcgattcgaa tgctgtagtc aaaacagtaa atgattgtta tttgtttgat
    16861 gggaaagaat taccagagtt cgttctaacg gatatttctg ggaaagaagt gactatccat
    16921 tgtcactatc gctttatcgg ggaattgaca ccaacaatga aagaattgtt ggaaacgatt
    16981 cgtcccatgg ctgaagtcac aaagcagctt tctgagaaaa atgacgaatt agaaagagaa
    17041 aatcatcatc ttcttgagga aaatacaaga ctagaccata cgttaaaaac aaccacaaat
    17101 cgctattgca cattgttgga aagtgatgaa tggacaataa aacatagact aggacgacgt
    17161 aagaaagaaa cttcgaaaaa aatccaagaa aaagaacttt ctatatgtat tgacgagaaa
    17221 atctgggatg cagaaacaaa aatattaaaa atcatcggat ggggaatcgc taattcggat
    17281 cgccagccct tgtcctataa gttatcagct gaccaatcgc catttttcga agcaattcct
    17341 gtttcacgag aagaagtcaa tcaagcagaa cagctcgcaa aaggaacaga agcgggattt
    17401 gagttacgaa tcctttgcga gcaagaacgt tcattcttag tggaagcagt agctcaaaat
    17461 ggccaatcat ggatcattga aatgtaaata atgattaact tataaagata aggagtaaac
    17521 gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca aatcactcgt gaacgggcca
    17581 ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa atccctacga
    17641 taaaggtaga aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga ttggatatca
    17701 atcatttata taatcttgat gttaagaccc aaagcggttt taaggtacgt ttatcaggga
    17761 aaatgagagg aaaagccatc ttagacttcc aaacagcgag acatcaaaat ggcatagctg
    17821 tgaaattaaa cggtaaatac ccttatgatg acggcattga atcttcatgg gaaagaaaaa
    17881 aaagattact gaaaaaaggg ataaattatg caaggactca tggcgtaaaa aaagcgatcc
    17941 gtcgggtcaa attggaattg aagccaggat cgatcgacta tgcagagtgg atcagtcgtc
    18001 atgagaaacc agatttgaaa gagcaaagga aagaagcaca aggatttgcg taccgtccat
    18061 tgatttccat acttatgcct gtctataacg ttgaaatcaa atggctcgaa aaatgcattg
    18121 attcggtact ggatcaaacg tatgatcatt gggaactatg tatcagtgat gatgcttcta
    18181 ctgatccagc tatccgaaag tgtctggaat cctatcaagc aaaagatgat agaatcaaag
    18241 tcgttttccg aaaagaaaat ggacacatca gtctggcaac taattctgca ttggaaatag
    18301 cagaaggaga atttatagca ctcttagata atgatgatga attaccgcca tttgcattat
    18361 atgaagtggc aaaagtgcta aacgtacatc cagaactgga tgtgatctac agtgacgaag
    18421 ataaaatcga tgcagatggc aatcgctttg atccgcattt caaagcagac tggtcgcctg
    18481 atacattgat gggaaataat tatatctctc acttaggcgt atatcgaaca agcattgtta
    18541 aagaacttgg cggtttccgt aaaggctatg aaggttctca agattatgat ttggtccttc
    18601 gtgtgacaga acaaatccct gcagatcata tttaccacat tgaccgagtc ttgtatcatt
    18661 ggcggacgat cccaggatca actgcaagta atggcgaagc caaaagttat atctatgatt
    18721 ccggagtgaa agcattgaca gatgccctgt ctagaagaaa catcaaagga agcgtacgtc
    18781 ctggcagaat ttctggattt tatgagattg cttatgacgt cttgcaagag gatttggtca
    18841 gtgtgatcat tccaacgaag aatggctatg aagatctaaa aacttgtgtg gattcgatca
    18901 tcgaaaaaac gtcttattca aattatgaaa tcattattgc ggataacgga agtacagatc
    18961 caaaaatgca agaattattt gcagaataca agcatcagct gaaagaccgc tttatcgtag
    19021 aattgattga cattccattc aattattctc gaattaacaa tttagcagca gaaaaagcaa
    19081 acggcaaata ctttttattc ttgaataacg atacagaagt gattgagcca gattggatga
    19141 cggtgatggt ttcttatgca cagtttgatc gaatcggctg tgtaggagca aaactgtttt
    19201 atccagatga tacaacgcag catgcaggag tattattagg aatcggtggt gttgcagggc
    19261 atgcattgaa taactatgat cgaacacatt gtggttattt cggccgattg gtcattgacg
    19321 tcaattactt agcagtcact gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg
    19381 ttaacgggtt tgatgaaaca ttagaagtag cattcaacga tgttgacttg tgcttgaaag
    19441 tttatgaact gggtcgttac aatgtctatg cacatcaagc agaattgtac catttcgaat
    19501 ctaaatcaag aggttatgaa gatactccag aaaaacaaag acgttttgct ggtgaaatca
    19561 aaaaaatgca agataaatgg ccagcatata ttgcgcatga tccattttac aatgataatt
    19621 taacaaaaga aggaatcggc gacttttcgt tgagaccaga ttaaactata ataaaaagct
    19681 gttatgttgt gattcgctcg cacataacgg ctttttatag ttttttgtca aaaaaaggca
    19741 gaaggtgccg actcaaaaaa ataagtcgag gttgggacag aagtgtttaa ctccgagaaa
    19801 taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcggct tatttaagaa
    19861 gaagttgctt ctgctcccac cgtttattcg tttttaaaga atgagacaaa agtgtttctt
    19921 acttttgtcc cacgctctta ttcatgctca gagcgccaat agatacaaaa tcatagccaa
    19981 agtttcacga accctagctg tcaaagaatc gatattttat tttaatatag gctattttaa
    20041 aataaatggt ttaaggtatt cctctacaaa agccactcta tcaattatca tgggtttaaa
    20101 ataagaagtg atggagataa ccagattgtt cttagcgtca atataaagaa catttccact
    20161 attaccaatt gctgaatata tttcgttctc agaatctaat acccaccata aatagccata
    20221 tctcatattt tgtaagtctt ctaaagcact ggttctaggt ttgaacatct cgtttatcca
    20281 catgggtggt acgatttgtt ttcctttata catcccttta tttaggcata atacaccaat
    20341 ttttgccaga tcacttgccg ataaacacaa accatatcct gccgtcccga tatttagtgg
    20401 atcacaaaac cagacattgg ttttaggaga cttatccatt gtaaactgct tatgttcttc
    20461 agctgtttca gcataataat tgacatggtt tggtatccct ataggatcaa atagatactt
    20521 attggcaaaa tcaacagttg ttagcttact tgtctgatac aacaagccag ttaatatgtg
    20581 taaacagaca gtttgatagt tgaattcgcc ggttatacct tttcttcctc ccaataaatc
    20641 tagtgatgtt tttgtccaat cttcacttga acagactttt gtccatggat cgcctttaca
    20701 tttgtagggt gctttcattg ttagcaagtg cttgattgta atagcgtaaa tagttttctc
    20761 cccgcgtttc actttatact ctggaaaata atctaatact ttatcatcga tacttttgat
    20821 taatccttgc tctaaagcaa tgccaatcag caaagataca atacttttcg ttgctgacat
    20881 gatatgacaa gtatcgtctt ttttataacc attccattca tctgtataaa cttctacccc
    20941 atccttatat gcgacaattt gacatatatt tggttgtcga aaaccaataa aatcatgcaa
    21001 ctcttgttta ttcataagat attacctcac tttcttctat aagcataatg tcattaaaaa
    21061 tcgatttcaa ttttttcgat tcttcacgta atacaggtat tcttatggac tagacagcgc
    21121 tttgggaaga tggattctct gaaaaatata aaggttcact ttatgagtgg ataaaaaaag
    21181 cagcaaatac ttcgacttct ttaatttacc aacattagtg aaaatcgatt catctggaga
    21241 tttgtaattt agagtccttg tcttgattag aggtgtataa agctttttat tagacatttt
    21301 taaattccat tacaatttgc ttataaacta ggtttttaaa ggtgactatg gtaagataat
    21361 agagatatta ttttgtgatt aggagtgtat atatgaataa aaatggagaa tggagtgctg
    21421 cacgacggat attcattata ttaatggatg ttattgtata caatgcagca atatatagta
    21481 gtttcttact gaagtttcgt ggagagatcc cattacgaaa tttagaaact ttccaacact
    21541 cagcaattat tatttcagtg atttttattg cgttgaacat tttgttgggt gcatatgttt
    21601 tttataatcg aatgatcagt gacatcgtgt ttgtcacagt catcggtcag gtattgatga
    21661 cactagggat catggtagtg acttttgcgg gccgctggtt tgcttttcca cgtcttgtta
    21721 tcttactttc atttgttctc ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata
    21781 aactttattt acgcgtaagt cgagataaaa aggtcgtcat tattgggtta gaaaaggatg
    21841 tcgcaccagc aatccaaaat tttggttcaa agaaaaacaa caaacataaa gtcgaagcag
    21901 tcattcttga taactattat gcaaacatca aaaatatgat taaagacatc gatattgtct
    21961 atcttgcttc tcatatcgaa gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa
    22021 atgaaaaaaa attgtttttg aatacaacat ttgaaaactt gacattgatc aatccaaata
    22081 ttatgaattt cgaagatgaa agtattattg aagcatctgg atttagaatc caacctgaat
    22141 atgaactatt caaacgtttg tttgatatca tcgtttcact tgttttatta gttattgctt
    22201 caccatttat gttattcaca gcaattttag tcaaagtgac atcaccggga ccaatcatct
    22261 ataaacaagt gcggatcaca aaaaatcaaa aggaattttc aatctataaa ttccgctcga
    22321 tgaccgccac agccgaagca aaatctggac cagttttagc taagagcaat gatgcacgag
    22381 taacgcctgt aggtaaattt attcgagctg tccgctttga tgaattacca caaatcttca
    22441 atgtgctgaa aggcgatatg tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg
    22501 atcagttcaa tgcagaaaac ccttattact acttacggca taatgttcgt gctggtatca
    22561 ctgggtatgc acaagtctat ggaaaatatg tctctgatta caacagtaaa ttacgttttg
    22621 atttacttta tatcaaaaaa tattcattga tgcttgattt aaagatcttg ctgcaaacca
    22681 tcaagatcct atttgataaa atgtcctctc aaggacttga agaagacgaa aatgtgatct
    22741 cattggcaga aattgctttt ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa
    22801 tgaacaatcc gaaattcagc ataatcgtac cagtatacaa tgcagaagca actattggtc
    22861 gaacgattga tattctaaaa gaattggatt atgaagagta cgaagtcgtt ttagtaaatg
    22921 atggatcaac agatcaaaca caggaaatca ttcaagaact gattgaaaat gatgaacgtt
    22981 ttgaattgat tactacgacc aatcaagggc ccggattagc aagaaacgaa ggaataagac
    23041 agtcaaaagg agaatatctc ttattcttcg atgcagatga cacgccagta aaaacgatct
    23101 tgtcagatta tgatcatttg ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt
    23161 ttgtttttcg tacaatggac gagggaagaa tcgtttccga aaaagaaaac ttagttacag
    23221 aatgtcacta tctaactcac gaagagttta taaaagacat gtatcagtta atgaatgaac
    23281 agttgatgta tgttgtatgg aacaaatgct atcgtagcga tattctgaaa aataaccaaa
    23341 ttctatttaa aacttataat agttgtgagg atcgaatatt taacttacat tattatgaat
    23401 actgtcatca agtgatcatg aacccaaaag tagaatatat ttatgaattt gaaggtggga
    23461 aggggatcac taatcaatac cgacctaaca agttcgaaac atttaaagaa ttctattcat
    23521 tagcaaatac tgtaacagac gatattaata aagacggtat ggcggcgctt cttttaaaag
    23581 ggacaacttc agttattttt tctatttata gtacctctgc aagaacttca aaagagaaga
    23641 aagacgaagc aaagcagata ttgagtgatc cagtgatccg agaagccaag caaattgcta
    23701 agacggattc ttcagcaaaa aaaataacaa agttgcttta caatatgcca gctccactat
    23761 ttttatctgc tgtaaaagtt ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg
    23821 ctttgaagcg agtatattag ttaatggagg aaaatataat gaaaagaatt ttgcattttc
    23881 agggacgaat gggtttaggt ggagcagagt ctttcatgat gaatttatat cgtaaaattg
    23941 atcgtacaaa ctatcaattt gattttttga tttatgaaga ttatgcagat gttcaagact
    24001 atcattcaga gattgaaaga ttaggaggcc g
//
LOCUS       DAZKOM010000033        23772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000033 DAZKOM010000000
VERSION     DAZKOM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.033"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8NF_001301"
     CDS             complement(164..388)
                     /locus_tag="ACG8NF_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0471790.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8NF_001302"
     CDS             complement(586..2166)
                     /locus_tag="ACG8NF_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0471791.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8NF_001303"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8NF_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0471792.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8NF_001304"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8NF_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0471793.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8NF_001305"
     CDS             5331..5765
                     /locus_tag="ACG8NF_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0471794.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8NF_001306"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8NF_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0471795.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8NF_001307"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8NF_001307"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0471796.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8NF_001308"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8NF_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0471797.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8NF_001309"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8NF_001309"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0471798.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8NF_001310"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8NF_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0471799.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8NF_001311"
     CDS             11001..11471
                     /locus_tag="ACG8NF_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0471800.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8NF_001312"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8NF_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0471801.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8NF_001313"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8NF_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0471802.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8NF_001314"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8NF_001314"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0471803.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8NF_001315"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8NF_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0471804.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8NF_001316"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8NF_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0471805.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8NF_001317"
     CDS             16164..16967
                     /locus_tag="ACG8NF_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0471806.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8NF_001318"
     CDS             17145..18092
                     /locus_tag="ACG8NF_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0471807.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG8NF_001319"
     CDS             complement(18160..18453)
                     /locus_tag="ACG8NF_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0471808.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG8NF_001320"
     CDS             complement(18481..19146)
                     /locus_tag="ACG8NF_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0471809.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG8NF_001321"
     CDS             complement(19286..19918)
                     /locus_tag="ACG8NF_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0471810.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8NF_001322"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8NF_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0471811.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8NF_001323"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8NF_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0471812.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8NF_001324"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8NF_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0471813.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8NF_001325"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8NF_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0471814.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         7051 a         4830 c         3825 g         8066 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aa
//
LOCUS       DAZKOM010000034        23749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000034 DAZKOM010000000
VERSION     DAZKOM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.034"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..654
                     /locus_tag="ACG8NF_001326"
     CDS             22..654
                     /locus_tag="ACG8NF_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471815.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(788..913)
                     /locus_tag="ACG8NF_001327"
     CDS             complement(788..913)
                     /locus_tag="ACG8NF_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0471816.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1047..1940)
                     /locus_tag="ACG8NF_001328"
     CDS             complement(1047..1940)
                     /locus_tag="ACG8NF_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0471817.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2122..3048)
                     /locus_tag="ACG8NF_001329"
     CDS             complement(2122..3048)
                     /locus_tag="ACG8NF_001329"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0471818.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3133..3894)
                     /gene="pflA"
                     /locus_tag="ACG8NF_001330"
     CDS             complement(3133..3894)
                     /gene="pflA"
                     /locus_tag="ACG8NF_001330"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0471819.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4138..6369)
                     /gene="pflB"
                     /locus_tag="ACG8NF_001331"
     CDS             complement(4138..6369)
                     /gene="pflB"
                     /locus_tag="ACG8NF_001331"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0471820.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6646..9096)
                     /gene="parC"
                     /locus_tag="ACG8NF_001332"
     CDS             complement(6646..9096)
                     /gene="parC"
                     /locus_tag="ACG8NF_001332"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0471821.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9108..11153)
                     /gene="parE"
                     /locus_tag="ACG8NF_001333"
     CDS             complement(9108..11153)
                     /gene="parE"
                     /locus_tag="ACG8NF_001333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0471822.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11319..11753)
                     /locus_tag="ACG8NF_001334"
     CDS             complement(11319..11753)
                     /locus_tag="ACG8NF_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0471823.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11880..12518
                     /gene="plsY"
                     /locus_tag="ACG8NF_001335"
     CDS             11880..12518
                     /gene="plsY"
                     /locus_tag="ACG8NF_001335"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0471824.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12650..13525)
                     /locus_tag="ACG8NF_001336"
     CDS             complement(12650..13525)
                     /locus_tag="ACG8NF_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0471825.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13607..14398)
                     /gene="codY"
                     /locus_tag="ACG8NF_001337"
     CDS             complement(13607..14398)
                     /gene="codY"
                     /locus_tag="ACG8NF_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0471826.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14416..15816)
                     /gene="hslU"
                     /locus_tag="ACG8NF_001338"
     CDS             complement(14416..15816)
                     /gene="hslU"
                     /locus_tag="ACG8NF_001338"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0471827.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15830..16378)
                     /gene="hslV"
                     /locus_tag="ACG8NF_001339"
     CDS             complement(15830..16378)
                     /gene="hslV"
                     /locus_tag="ACG8NF_001339"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0471828.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16415..17320)
                     /gene="xerC"
                     /locus_tag="ACG8NF_001340"
     CDS             complement(16415..17320)
                     /gene="xerC"
                     /locus_tag="ACG8NF_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0471829.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17518..18894)
                     /gene="trmFO"
                     /locus_tag="ACG8NF_001341"
     CDS             complement(17518..18894)
                     /gene="trmFO"
                     /locus_tag="ACG8NF_001341"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0471830.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18863..20941)
                     /gene="topA"
                     /locus_tag="ACG8NF_001342"
     CDS             complement(18863..20941)
                     /gene="topA"
                     /locus_tag="ACG8NF_001342"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0471831.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21063..21920)
                     /gene="dprA"
                     /locus_tag="ACG8NF_001343"
     CDS             complement(21063..21920)
                     /gene="dprA"
                     /locus_tag="ACG8NF_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0471832.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21976..22743)
                     /locus_tag="ACG8NF_001344"
     CDS             complement(21976..22743)
                     /locus_tag="ACG8NF_001344"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0471833.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22736..23596)
                     /gene="ylqF"
                     /locus_tag="ACG8NF_001345"
     CDS             complement(22736..23596)
                     /gene="ylqF"
                     /locus_tag="ACG8NF_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0471834.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6180 a         5125 c         4079 g         8365 t
ORIGIN      
        1 tcaatttaat ttggagatat tatggaaatt caacgtagaa ccactaattt tttacattta
       61 ggctcttttt ttgcaacttt gacgatctta ttttttctat gttattcata ccttaagacc
      121 tcacatgcgc cttctagtgc attaggctgg ttgcttttgg atcattctga tatcacagaa
      181 gccgaagaaa atccgttttt tttgattttg tcttctcttt gcggcttact gttttctgtt
      241 tatttcggtg cctatttttt tcatcgacat cctggtacgt taaacgaagc ttcaagcgta
      301 ttgattgcat ttaaaacgtt ttctttttta gcaattcttt tttattcgtt taatcacaat
      361 acgcttttct tctttatcat gaatggttta gtcgtgattt tatccatttt tacttatttt
      421 attttttcac tcatttttag ccaattgaaa tgtccgctct tctttcgatt agttcctgtc
      481 acctgtttta tttacagctt gttttccttt tttgtccttg ctttaatgcc actgaccaac
      541 ccttcgacga tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt cagtctaggt
      601 gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc ctgactatta
      661 cgacttcata aatgcttttt tagttttaaa cacttcaatc taaaagaata acccaagatt
      721 ggataaatta ttaatgactc gccattcgtt tcttagtgat tctggattat caaatggact
      781 tcctaatcta atcagctagt tcttcccata aaatgccata cttgactgaa tcataatatt
      841 gattatttaa aaatctaact tttcttatca ctacttcctt tgtcattcca catttctcac
      901 catttttttg cattcctttg tttccagacc atgtagtgaa tctaataggt ggtagatagg
      961 caaaatactc aaataattct tttaaccata aaggaagaga aaggctcctc ctgcaagagt
     1021 cagtataatt tttgcttact gctctcttag gttttactca ctaaaactgt tccatattct
     1081 ttgatataag aaaatgcatg cggtataaat ttcttgctca gctcagaaaa gatttgtcgt
     1141 ttaagaaagg ctatttcctc cattgtcaat gaaggatcca atgttggtaa ttcaatctca
     1201 aaattgtttt tttcgacaat aggtaattct aatgtgaatg attcttcttc ctttatagta
     1261 ttcgtcacaa gtagagactg atggaaaata ctaggccggc gatttttata tttattctta
     1321 cgtatcctgt caacgataac ttctatttga tcaaaaaagt acgattcatt tacgtctcca
     1381 ataagttcgg ctaattcttt gaatgccttc gttacaggaa gctgattctt ctgtacaaaa
     1441 aagtaattag ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat aaaacggtac
     1501 ttctcatttc ctgcagttgt cctcagcaat tctgtctctc ggctgtttct tactccataa
     1561 aaatacgctt ctttccgttc atcttcgaaa caatacgccc aaataacttc tcccatcgct
     1621 aatttttgtt catcaacaga gagtctcttc aattgttcag ctatggtttc agcagccgaa
     1681 gtggcttgtt gcaagtaatc tttaggatca aatattggaa agttcaaatg atatcttcgt
     1741 tcttccctct tgattaatcc tgcatcgatc aataaatcaa gaaaatggga aaatttattt
     1801 tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt gttctcgatg ttcatataga
     1861 taatcggtca atgaagaaaa aagcggatca tcggtcagtt tttgataaaa gttttcctta
     1921 ttttttgaat aatagttcat atatgttctc ttttctatcc gttttatttt acttaaaagg
     1981 cttttacgta aaaaggagga agaacaagag tcataattga ctgttattct cctccatttc
     2041 ttcggccttt agtatgttct atgtttcttt ttatatcaat cgcttatcca ttcattttga
     2101 atgcttcata ctaccatttg attagctaaa tacagccgtc aattgaggta cgacttgttt
     2161 tttacgtgaa acgacacctt taagcaaagc acggttgttg gtcaaagttg tttggaaagc
     2221 ttcttctact ttttcttttc ctttaccagc aacaagtaat tctgaatcac tgtctaagat
     2281 gtttgtgaca actaatacga acagatcgta gccatttgct ttcatttcat cttccattgc
     2341 tgcttctagc tctgcttgtc gatccatcac ttcattaagg tccactgtat tgacttgagc
     2401 gatacgaacg ttggaactgc ccattgggaa actttttgca tccaaatcaa gaagaaccgc
     2461 agctgattta tcacttagat ttgttcctgc tttcaacatt tcaagtccat aagtgtttag
     2521 atcgacttca gcgattgctg caagttcttt tgcagctgct tcatcttcag gcgtacatgt
     2581 tggtgatttg aacaacaatg tatctgaaat gatagctgag agcatgattc ctgcgatttc
     2641 tttaggaatt gttacttggt tttctttata tagttttaaa ataatcgtac ttgtacatcc
     2701 aactggttct gcacgatagt acaatggatt agctgtttca aagtttgcaa tacgatggtg
     2761 gtctactaca gctaaaatat ttacttcgct gatatctgaa atactttgtt ggaattcatt
     2821 atgatccaca agcatgacat ctgcattttc tgctttagct gactctacta ctcgtggtgc
     2881 agatagctga aagtggtcta atgcgtattg tgtttcttca ttaggtgtac ctagagcaac
     2941 tgcttctgtt tctttaccta attcattttg caagtgtgaa aacgcaatgg ctgccccgat
     3001 tgcatctgta tctggatttt ggtgaccaaa aactaaaatt tttgacatgt cattcgctcc
     3061 tttgtgattt ctcctattta ataatagcaa aaaaacggaa agaagcaata cttatcttct
     3121 ttccgatgcg acctatcgcg ttaaatattt tgtatactct tctgtatgaa gcaatcgttt
     3181 agcattttcg acacgttctt ttgtaggtgg ttcgatccct tctaatggat atttcaaacc
     3241 aagtgtttcc cacttgtatt ttcccatcgt atgataagga agaatctcta ctctgtctac
     3301 atttgacaag gtttgaataa agttgtctag tcggatcaaa tattcatcat aatcacttcg
     3361 ctcaggaact agtacatggc gaatccaaac cggtttgttg atttttgaca aatattgagc
     3421 catttctaaa atattttcat tgtcccaatg cgttagtttt ttatgctctt cattatcgat
     3481 gtgtttgatg tcaaataaca gtaaatctgt atacttcatc aattcttgaa aacgtgaaaa
     3541 gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat gcatcttccg
     3601 ctcttttgct tttttaaata gttcgatcaa aaaatcgatc tgcaatagag gttctccacc
     3661 actaacagta ataccacctt tatctcccca gtaaggtctg aagcgttctg ctttatctaa
     3721 aagttcatct gctgtgtatt ctttccctcc gccaatattc caagtatctg gattgtgaca
     3781 aaattggcaa cgcatgcggc atccttgcat aaacacgaca aagcgcagtc caggtccgtc
     3841 tactgaaccg aaagtttcga tagaatgaac atagccgatt gttttttctt ccatttaagt
     3901 tcacatcttt tctttgattt cattttaagt acaagttagc tgtcgaagcg aagctttctg
     3961 aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga cggaacatac
     4021 aagtcctgcc cctgtaaatc gtttctttca aataagcata ctgattattc gattttgatg
     4081 cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac gaacttttta
     4141 cattctgtcg tgtgaagttc tcatcaagac atctaattgt tgttctcttg tcaagtcacg
     4201 gaatttaact gcataaccag atacccggat cgtcaagttc ggatatttca ttggattttc
     4261 cattgcatca cgcaataaat cattactgaa tacattgaca ttcaaatgat aagctccttt
     4321 tgagaagtat ccatctaaga cattacgcaa gttttcgatt cttgtatctt gttcttttcc
     4381 aagacctgaa ggattgattg tttgggtatt tgaaattcca tccaatgcac agccgtaatt
     4441 cattttagct gttgagttca ggctagctaa taacccactt tgttccgcat tgtaacttgg
     4501 atttgctcct ggcgcaagtg gttttccagc ttcacggcca tcaggagtat ttcctgtatt
     4561 tttaccgtaa acaacatttg aagtaatcgt caataatgaa gttgttggtt ttgagttgcg
     4621 gtaagtttta tgacgtttga tttgtcccat gaagtattcc aagatccatg aaactaattc
     4681 atctgcacgg tcatcatcat ttccgtattt agggaagtcg ccatccactt caaaatcaac
     4741 tgctattcct ttttcattac ggattgcttt tacttttgca tatttgatcg ctgacaagct
     4801 gtctgccgca tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca agtcagaatc
     4861 catcaaagcc agctgagctg cttcataaga atatttatca tgcatataat gaatgatgtt
     4921 caacgtattg acatatagtt ctgctaacca atccatcata ttcatgaagt tatgtttcac
     4981 ttcttcaaag tctagtattt ctgatgtgat tggacgatat tctggtccaa cttgggcgcc
     5041 agtcacttca tcgactccgc cattgatggc atataatagt gttttagcca agttagcacg
     5101 agcgccaaag aattgcatgt cttttccgac aactgttcct gatacacagc aagcaatcgc
     5161 acaatcatca gaaccccatt gttcacgaag ctgattatca ttttcgaatt gaatcgaaga
     5221 actttcgata gcgattctgc tagcatatgt tctgaatcct tctggaaggt gttcagaata
     5281 caaaacagtc aagtttggtt ctggtgaagg tcccatattt gttaatgtat gaagcagacg
     5341 gaaatcattc tttgtaacta atgaacgacc atctaatccc atacctgcaa tggataaagt
     5401 agcccagata gggaatcctg agaataattg gttatattca ggtgtacgcg cgaatttgac
     5461 catacgtaat ttcattacta agtggtcgat caattcttgt gcttcttttt ctgtgattac
     5521 tccattatcc aaatcacgtt ggatatagat atcaaggaat gctgagatac gtccgattga
     5581 catcgcagca ccattttgtg atttgattgc tgctaaatat ccgaagtata gccattggac
     5641 agcttctttt gcatttgcag ctggtttaga aatatcatag ccatatttcg cagccatttc
     5701 tttcatttgc gctaatgcac gatattgttc agtgatttct tcacgtaaac gaatcacttc
     5761 ttcactcatt gtatgataac cagtcatatc atgatctttt ttcttttgtt ccatcaaata
     5821 gtcgattcca tacaatgcca cacgacgata gtcaccgatg atacgtccgc gaccgtaagc
     5881 gtctggaagc cctgtaatga ttttgttttt acgagcagca cgcatttcag cagtataagc
     5941 gtcaaataca ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg tcaattcatc
     6001 gtttggtgta tagccattgc tttttagcgc attgttagcc atattgattc cgccgaacgg
     6061 catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt ccaaatcttt
     6121 atccaaatag cctgcatcgt gtgaagtaat agttgatacg atttctgtgt ccatatcata
     6181 gacaccgtta cgctcgaatt ggatatcatt taacttttgc aattcatccc ataatttttc
     6241 tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg cgatattcag tgtagttatt
     6301 ttgaataaag tctctagtgt ctacgccttc tttccatttg gtacctttaa atccattcca
     6361 ttgctccatt gtatggacct cctaaatttc tgttttatgt aacagttttt acgtgattag
     6421 tataacacag caccctatct ttgcaagggt tttcttgttt tttatttaac aatttttatg
     6481 ttttatttac atatatttaa atcaacgttg acataccaac atttaagcat tgtgaacact
     6541 ttcatctttt tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa gagacgccac
     6601 ctgtattaat acagatggcg tctcttcatt aaaatactac aacagttacg actcgatgaa
     6661 aatctcagga gcatcgataa cttcgatgac ttctccacct tttttctcat ccatcgcaaa
     6721 tgatccattc gatgttcgat cacctatagg gaaatcagtt gggtcaatcg ttaactgctg
     6781 ccctttttga ttcatcaagg ttatttcttt tgatcttcct tccgacatat agataatacg
     6841 gtgcggattt tttttcagct cacgcatgac cgtcactccc cgcttcgcac gtgaagtttg
     6901 gctgatttct tgagcaagca tgcgtttaac agctcctcgc tgagtaacga ttacgatcgg
     6961 cgtatcacct tctgctaaca caagcagccc gttgataatc ctatctccat ctttcaaatt
     7021 catagacttc actccagctg cctttgctcc aacaacggga acatctgtta aaggatagcg
     7081 caaacccatt ccttgatgcg ttactaagaa aacatctaag cctggctgat cttctttcag
     7141 atacacagca atcagttcat cttcttccgc ctttagcttc atacagctga gcggacggct
     7201 cttataggtg cgccatggtt cgaactctgt cattcgtgtt tgcttgatca atccattctt
     7261 gctgatgaat acaaatgttt tctctgcttg aattttttga tatgggaaaa cagctaaaat
     7321 cgattcgtct acagacagat tggagattgt ttgagaaatg tgctcaccag catctttcca
     7381 tttcaaatcg ggtagttcat ggacagggcg gtagatcaca tttgctttat ttgtgatcag
     7441 aagaacatgg tccaatgtac tcaattcacc tgagtatagc aagtagtcgc cttctcgcat
     7501 cccgatttct tctggtttgg aagcactata agaacgaagg cttgttcgct tcacatagcc
     7561 ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact aagacttgcg tatcaatttt
     7621 gatttcttcg atttcgtctt cgattgtcgt tagacgagcg gaagaataat tttttttcac
     7681 ttctcgcaat tcttttttca taacagagaa aagctctttg tcatttgaaa ggattttatt
     7741 caactctgtg atttgcgcaa tcaagctttc ggattccttt tgaagctctg tgatatccgt
     7801 gttcgtcaaa cggtataatt gcaatgtgac aattgcttct gcttgttctt ctgtaaattg
     7861 aaagacgtcg actaaattct ttttggcgtc ttttttgtct ttactttcac gaatcgttgc
     7921 gattacttca tccagaatcg ataaagcttt catcaatcct tctacgatat gctgtctttt
     7981 ttgagctttg gctaaatcga attgactacg ttttgttatg acttgctttc gatgctcgat
     8041 ataacttctt aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt cgatagcgac
     8101 catattgaaa ttgtaattga tctgtagctc agtgtttttg aacaaataat tcaaaattcc
     8161 ttgagcattg gcgtcttttt tcaattcgac gacaatttgc agacctgttc tatcactttc
     8221 atctcgtact tcagctattc catcgatttt cttattcaaa cggatctcat ccattttttt
     8281 caccaaggtc gctttattta cttcatatgg gatttctgtg atcacgattt gctgtttatt
     8341 acccttcatt gcctctatct ttgttttcga acgaagaata acttttcctc gtcctgtttc
     8401 ataagctttt ttgatttcat cttttccttg caagatccct cctgttggaa agtcaggtcc
     8461 aggaatatat tccatcaatt tttcaaggct cgcttgtgga tgatcgatca tatagattgt
     8521 gccatcgatc acttcagcta agttatgagt cgggatttct gttgcatatc cagcagatat
     8581 acctgtcgat ccattgacga gaaggtttgg gtatcgagct ggaaggactg ttggttcttt
     8641 ttctgtatca tcaaaattcc acacgagatc gactgtttct ttctcgatat ctgctagcat
     8701 ctctccactt aacttggata atctggcttc agtataacgc atcgcagcag gtggatcacc
     8761 gtccatactc ccgttattcc cgtgcatttc gatcagtact tcccgcaact tccagtcttg
     8821 gctcatgcgg accatcgctt cataaatact gatgtcacca tgaggatgat aattccccat
     8881 gatatttccg acagattttg ctgatttacg gaatcctttg tcatacgtat tgccatcttt
     8941 gttcatggca aataatatcc ggcgttgcac tggcttcaat ccatcacgga tatcaggcag
     9001 tgcacgttct tggataatat atttggaata acgtccgaag cggtcaccca tcacttcttc
     9061 taaagttaat tcttgtactt cttgtcgatt ttccatcagg tgacctccta ttctacttca
     9121 aataactgat tgttttcatt tttatctgct cgttcttcat ctaataaatc attagaaact
     9181 gaaggagaga tttcagtatc ctctttttta tctaagatac tgccatcttc ttcgagcgta
     9241 aattgcacgt gattttcgat ccattttctt cgaggttcta ctttgtcacc catcagtgtg
     9301 gttactcgac gttcagcttg cgcagcatca tcgatacgga cgcggatcaa cgtacgggaa
     9361 gtcggatcca tcgttgtttc ccacaattgt tccgcattca tctctccaag tcctttatat
     9421 cgctgcagca tatatccttt gccgactttt ttaatcattg ctgccaattc gtcatctgtc
     9481 cacgcatatt caatgacttg cttctttcct tgtcctttgg atactttata taaaggcggt
     9541 aacgcgatat atactttccc agcttcaatc agcggtttca tataacgata aaagaaagta
     9601 agcagtaata cttgaatatg ggcgccgtct gtatccgcat cggtcatgat gattactttg
     9661 tcgtaattac agtcttcgat agaaaactca ggaccgactc ctgcaccaat cgtatagatc
     9721 atcgtattga tttcttcatt tttcaagata tcttgcattt tagctttttc ggtattgatg
     9781 acttttcctc gtaaaggtag aattgcttgg aactttctgt ctcttccttg tttagcagat
     9841 ccgccagcag aatccccttc gactaaatac aattcatttt tcttagggtt tcgggattgt
     9901 gcgggtgtca attttcctga aagaagagat tcgccttttt tacgcttctt cccatttcgg
     9961 ctttcttctc tggctttacg tgctgcttct ctcgcttctc tggctttgat tgctttacgg
    10021 accaacatct ggctcatttc gctattttcc tgcaaataaa agcccatttg ttcactgatg
    10081 acgttatcta ctacggtacg agcaactggc gtaccaagtt tttcttttgt ttgcccttca
    10141 aattgcagaa gattttcagg tacacggata gaaagtacag cagctaaccc ttctctgaaa
    10201 tcactacctt ccagattctt gtcccgttct ttcagcaaac caacttttct cgcatattca
    10261 ttataagatt tcgtcatcgc cgctttcata ccagcttcat gggttccccc atcttttgtc
    10321 cgaacgttat tgacaaaaga taaaacattt tctgaataac cgtcattgta ttgataagcg
    10381 acctctactt cgataccttc tttttctcct gaaaaataaa caactggtgt caatgtatct
    10441 ttttcttcat tcagataatc aacaaattct ttgattcctt cttcgtaatg aaaaatttcc
    10501 ttgactggtt cttctccacg aagatcagaa agagagattt taacgccttt tagaagaaag
    10561 gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt actgaatatt
    10621 gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt tcctatcttt
    10681 ttcaaagtac cgtcaggttt accgccgttt ttaaatttct gctgatactc tacgccatct
    10741 ctaacgatcg ttacaattag ccatttcgat aaggcattta ccacactggc tcctacaccg
    10801 tgcagtccac cagaagtttt gtaccctcct tgaccaaact ttccgccagc atgcagcaca
    10861 gtaaaaatca cttcaactgt tggtattccg gaagcatgca ttcctacagg catcccacgt
    10921 ccgctatctg caacagttat actattgtct tcatggatgg tcacatcaat ctcactgcca
    10981 taacctgata atgcttcgtc aactgcatta tcgacgattt catagactaa atgatgcaag
    11041 cctcggctgt cagtggatcc aatgtacatc cctggtcttt ttctaactgc ctctaatcct
    11101 tccaatactt ggatggacga gtcattgtat tcgttgttta ctttttttgc caaggcaaac
    11161 gctccttcaa taatactttg cggcaaaacc gcaccacgat tatcatacta gaaaaggtcg
    11221 tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat ctcctaaccg ctttttcgac
    11281 attcccctct atcgatcatc tcttctttgt ggtcattttt atttttggtt ttctttcaac
    11341 ttaccaagtt caatcttaat acaacgatcc attacgatat ctgtacgtcc tgcttttctt
    11401 agcatctcag ctgcttcttt gttttcaatc cctaattgtg cccagaacac tttagcatca
    11461 gttttcaaaa aatcacctgc tatttcaggt aaaaattcac tccgtctaaa tacatctacg
    11521 atatcgattg gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc tagaatttct
    11581 tcaccagcca ataatggatt tactggtatg attttatatc cattgtcttg caatacctgt
    11641 gcaaccatgt aacttgttcg ttctggttta ttgctcaagc ctacgacggc aatatttttt
    11701 gcttcttcca aatagttgaa aatcgtttct tgtgttggat tttttacaga catttctgcc
    11761 accccttgct tttcttttta ttccatctat tatacttgta tctttcattt tttcaattgt
    11821 tcctgttacc tattcatgct aaaataaagg atagttttca acttgaaaga gggtaaaaaa
    11881 tgaaattgat cttattactt attttagcat atcttttagg ttctattcct tccggtgtct
    11941 ggattggcaa attgtttttc aaaaaagata tccgacaaca cgggagcggt aatactggaa
    12001 cgaccaatac atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg cttatggata
    12061 ttttaaaagg tacattggct acttgtttgc cgatgatctt ccacctttct gggattaacc
    12121 ctctttggtt tggggtatgc gccattcttg gccatacatt tccgattttt gctgggttca
    12181 aaggaggaaa agctgttgcg acaagtgcag gaatgctttt aggatttaat cctgtatttt
    12241 ttgtttattc ttgtatcatc tttatcgtta gtttattttg tacaagtatg gtgagtttga
    12301 gcagtatgat cagtgcagta ttgatcactt tatctacgat cgtccttcct tatgtagctc
    12361 ctgtgatttt ggctaagccc aattggttac tgacgatcat tgccttttta gtttcttcct
    12421 ttatctttta tcggcataaa gacaatatca agcgtatcag aaaccatact gaaagtcgaa
    12481 tcccgtttgg tttgaatagc agcaagaaaa aatcctgata ctttttcatt ccaaagagat
    12541 aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt acttacagga tagctttttt
    12601 acacaccttg gtaaacggtg ttccctgcta acttctcggt cacaacctct tacttcactg
    12661 tgatggaata ttttgtcttg aagatttcat gggcacctag ttgattgatc cctacttttt
    12721 ccatcaagtt tccactactg ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa
    12781 cgaaaggcgc ttcttttgga taaggagacc agatccctac ataaggcata tctttatagc
    12841 ttaaagttac gctatgggaa gatttttcac ttcgaatggc gtacgcattt agaccccttg
    12901 tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg
    12961 tctgacctaa cgtcttttga tccatatcga tgaatgggcc tttcaatggt aggcgtaatc
    13021 ttgatttcaa tggagaaaat gccagataga aatcttcaaa tgacaatcct tcttcaagag
    13081 gtacattgaa agcaggatgt ccaccgatag aaaagaacat ttctttttca ccggtatttg
    13141 ccacttgata atgaaccgtg atgccgtctc ctcctaattc atagctgatc actaattcaa
    13201 aatcaaatgg atacttttct aatgtctccg gcgtactcct taagatgaaa ctagccattt
    13261 cttgtccatg ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt tgccccattt
    13321 gatatgtttt cccttgataa acatactgat catcttttaa acgtccaaca aaagggaata
    13381 aaaccggtgc atgtctgccc caatacgctg gatcaccttg ccagatatac tctaaaccat
    13441 gttcttttga tttcagactg atcaattccg caccgtgttc agcgatttcg gcgatcagct
    13501 ggtcattttg aataataaca gtcattgcca ttcctccaac ttcgacttac tttttaaaat
    13561 gggaaggctg gaacaaacat ttctatctgc cccagtcccc ttacttctaa taactgtgtt
    13621 ttgctaactc gtctttaaaa cgggaattca atacttttaa ataagttccc ttcatgccta
    13681 acgaacgaga ttcaatgatt ccagctgatt ccaattttcg taaagcattg acgatcacag
    13741 aacgagtgat gccgatacta tcggcaatat tagaagcagt cagacgacct tcatctccat
    13801 ccaatgcttc aaagatagct tgtacagcct tcaattcgct ataagataat gtgttgattg
    13861 ccatttggac agccgttgca ctccgtacat ctgcttcgat gcttcgagac ttctgataaa
    13921 ggatctgcat accgacaacc gtagctccat attctgctaa gactagatcg tcatctgcaa
    13981 acgattgttc catacgagat aagataatcg tacccaaacg ttcgccagct ccaaagatcg
    14041 gaacgacagt cgtcagacca aatggatatt tctctcttga ctctaccgga aatgcagtaa
    14101 tatcactagt aattgggata ttagcttcag ttttcatcaa ctgtgccact gtatcagtat
    14161 aagattcagg aaattgtttc tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt
    14221 gacgttcatt ataacctagt aatactccgt cacttcctat gatataggtg ttgctgtcaa
    14281 gaataccacc tagtgtgaca gccattttat cgtaaggcaa atctgcttgc atgtcaaaag
    14341 tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag tctgtcattg
    14401 tcgtcctccg attttttata aaatatagcg acttaaatct tcatttgccg caattgagcc
    14461 aagtttgtca tccacatatg cttctgtgat cgttatttct cccatttgca tatctggtgc
    14521 ttcaaataat agatcttcta gtagtttttc caaaatggta tgcagtctgc gtgcgccgat
    14581 attatctgtg tcacggttga catcgaaagc gatttgagcg atccgttcaa ttgcttcttt
    14641 tgtaaacgta actgacacat tttctgtacc gatcaacgcg acgtattgtt ttactaaagc
    14701 attatttggt tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat ctaattctac
    14761 ccgtatcggg aagcgtcctt gaagttctgg aattagatca cttggtttag acaaatggaa
    14821 agctcctgaa gcaatgaata ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac
    14881 ttgtgaacct tcgacaattg gcaatatgtc tctttggacg ccttctctgg atacttctcc
    14941 agattgctgt gatttagaag tgatcttatc gatttcatcg ataaaaataa ttccactttc
    15001 ttccgctaat ttgattgcct cactatggat atcagcatcc ttgactaatt ttgctgattc
    15061 ttccttgatc aataactcta aagcttcttt gactgttacc gtccgttcca cttttttctt
    15121 cggtgtcaaa gcacctagag tttcatttag gtcgatcccc atttgctcca atccattgtt
    15181 catcattggt gcaggtttct tttggtcttc aacttcaatg gttacttcac gactatccag
    15241 taaacccttt tgtagttgtt ccaagactgt acggcgattg attcgaatat cttctgtcac
    15301 ttcttcttgc ggttcagcgg tattttgctg actagcatta aaaagattca tcatttgttc
    15361 aaattgatta gtggattgtt tctgttcttt tttgatacca ggtaccaaga tttttaccaa
    15421 acgtttttct gcattttttt tagcacttgt gtatacacgg ctgtactgct ctttttctac
    15481 gatttggatc gcattttcta ctaaatcccg aaccattgat tctacatctc ttccgacata
    15541 accgacttca gtaaattttg ttgcttcgac tttgacaaat ggtgcattga cgatttttgc
    15601 caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt ccgatcatca gcatattttt
    15661 tggtgtaata tcttgttgca ttttttcatc taactgcaaa cggcgatagc ggttacgcaa
    15721 agcaacggca acagattttt ttgctgcgtg ctgtcctacg atatattcat ctaattcact
    15781 tacgatttgt cttggtgttt gttttaattc atacatatct actcacctct tacagttctt
    15841 ctacgataat attatgatta gtaaagatac agatatctgc ggcaatattt aacgcatttt
    15901 ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt tttcattgct cgggctgcag
    15961 ccaacgcaaa attccctcct gatccgatag ctaagatacc atcatctggt gtgataactt
    16021 ctcctgtacc tgaaacaaga agcatttctt tatcattcat tacgatcagc atcgcttcaa
    16081 gtttttgcat agattgctgt gtccgccatt cttgagctaa ttcgacagct gcgcgttgta
    16141 gattcccgtt atattcgttt agtttgcctt caaatttttc ttctaaagta aacgcatctg
    16201 caacacttcc ggcgaaacct acaacaactt ctccattgta gatacgtcgt actttctttg
    16261 cagtgccttt catgacaaca gattcaccca ttgttacttg gccatctcca gccatagcaa
    16321 atttcccgtc tttttcgacg gcacagatag tggttgaatg aaatggtgat tcgaccatgt
    16381 ttgtttcctc ctaaatttgt tcttttcctt atttttacgc tcttggatgg aaagaacgat
    16441 agtttttttg taaactttct tttgttacat gggcatagat ctgggtagtc gataaattgg
    16501 catgtcctag caattcttgg actgtccgca aatctgcccc attatttaat aaatgagtag
    16561 caaatgtatg gcgcagcatg tgaggatgga tatcactatc caaactgctt tttttgatca
    16621 tttgattcaa gacatattca attccagtag gcgtgattgg ttttccatga tgattcacaa
    16681 aaacgatatc atgctcttcg tgatattttg tcatcaatac ctggcggcct tccctgaaat
    16741 attcttgcag agcatcttga gcaaaagaac ctagaggagc atatctctct ttgttccctt
    16801 taccatggat caacatgacc tctgaatcaa aatcaatgtc tgataccgac aaactgctac
    16861 actcacttaa acggatgcct gacccgtaaa ggacttccag taaagcgcgg ttcctcaaat
    16921 ccaaaggact atctcctgct acgctgtcaa aaagtgcttg catttcattt tcataaaaaa
    16981 aacgtggcag tctcaaattt ttctttttga gatgaacgta agaaaatgga ttttctttta
    17041 tcacttcttc tttgagaaga tactgataaa acgaacgtag gctggctatc ttgcgactga
    17101 tagaatttct gctatagcta ggacgatgat cagctaatgc actcaaatag acacgaaccg
    17161 taaaatattc cgccttcagc agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat
    17221 ggagaatgtc ttcttcatat gcttctttcg ttttttctga atatcctctt tcgacaatca
    17281 aataacgcaa aaatttttgt tcccacgtgg cttctgtcat tttctcacct tccttttgtc
    17341 ccagagaata atttagcaca gcgtttataa aaagacaaat aaattgtcag aatttaacca
    17401 atatttctaa gtttgttcac aattggcgca attcctcaca ttttgtttga gaaattctta
    17461 agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg agtttcctta
    17521 ctcatttggg attgcagttt tttctaattc tgtttttatt gcctcattag cttttaaagc
    17581 tcgttgtgca attgcttcat atcttgcttt tttgtctcga atgcgctctg gcagttcagg
    17641 aaacagacca tagttggcat tcatcggttg gaaatgtttt ccttccgcat gagtaatgta
    17701 gtaagccata cttccaatag ctgtttcttg tggaaactcg actggttcca tttcttttgc
    17761 taagcgtgcg gcgttgattc cagcaagtag tccgctcgct gcactttcaa catagccttc
    17821 tacaccagtc atctgacctg caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg
    17881 tttcagcaat tctggcgaat tcataaagct gtttcggtgc atcacaccat agcgaacgaa
    17941 ttcagcattt tctaacccag gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa
    18001 gtgagtctga aagcccacga tgttgtataa agaagctgca gcattgtctt gacgcagctg
    18061 gataacagca taaggacgtt tgccagtttt aggatcttcc aatccaactg gtttcatagg
    18121 tccgaaaagc atcgttttga atcctcgctg tgccattact tcaattggca tacatccttc
    18181 aaaatatttt tctttctcaa aagatttaag ttctgccact tcggcattga ttagtgcctc
    18241 atggaaagct ttgaattcct cttctgtcat cggacagttc aagtaagctg cttctccttt
    18301 gtcatagcgg gacttcaaat atactttatc catatcgatc gtagctttgt ctacgattgg
    18361 tgcagctgca tcatagaaat aaaaaccatc tgaaccattg aatgctttga tgctttctgc
    18421 taatggatga gaagtcaaag gtcctgttgc tactacggtg attccttccg gtatttcagt
    18481 aatttcttcg tttatcactg ttactaatgg atgctctttg acttttcggg taattgtttc
    18541 agaaaaagaa tcgcggtcaa cagctaaggc accaccagct ggcactgctg tttcatctgc
    18601 cgaagtgatt actaccgaat tcaatcggcg catttcttct ttcaataacc ctactgcatt
    18661 agccaaactg tttcctctta atgaattgga acatactagt tcagcaaaat tacttgtgtg
    18721 atgcgcctca gtcgatttga ctggacgcat ttcatataaa cggacaggga ctcctgcttg
    18781 tgcgatctgc caagcagctt cacttccggc aagccctgct ccaacaacat ttaccatttt
    18841 caatgaaaga acctcttttc tattattttt ggatgtcttc ttcgtaatct ccatttacac
    18901 agactacttg tttaccgcct ttgactttct tctcgaccag atatccgtca cattttggac
    18961 aatttctgcc cactggttta tcccaagaag tgaattcaca ttctgggaaa cgggaacaac
    19021 catagaatag tctgtttttc ttagattttc gttcaatgac ttgtccttca tgacatactg
    19081 ggcaagtcac accgatttct tttacgattg gtttcgtatt ccgacaatca ggaaaattac
    19141 tgcaagcata aaacttccca tatttcccta attttatcac catcggatgt ccacagacat
    19201 cacaatcgaa tcctgctggt tcatctttga tttgaatttt ttcgattttt tcttccgcat
    19261 tcgtcagttc tttttcaaac ggtttataga aacgatcaac cactttgacc cacgcttccg
    19321 tgccttcttc aattttatcc aaatctcctt ccatttctgc tgtgaaatgg atatcgacga
    19381 tttgagggaa gaattcacag attaatgaat tgacaatttc tcctagttca gtcggctcaa
    19441 atcttttttg tgtcaatttt acatagtagc gtctttggat cgtatcaagt gttggtgcat
    19501 aagtggaagg tcgccctacc ccgttttctt ctagtgcacg aatcaacgta gcctcactaa
    19561 aacgtgctgg tggttgagta aaatgttgtt ttggttcaat atcaacagat tggactacat
    19621 cgccttcttc aagttctgga agaatatttt ctttctcttc tttgccgtca tctctacctt
    19681 ccacatacac ttgcatgaat cctttgaatt tgatttttga accgttcgca atgaagatga
    19741 caccattttg ttctaatgtc actttcattg tatctaaaat agctggtgtc atctgactag
    19801 ccacaaaacg tgaccagatc aatgtataaa gtttcaattg atctttatct aaatattgtt
    19861 tgagttcatc tggtgtacga agcacactcg ttggacgcac agcttcatgg gcatcttgtg
    19921 cgccttgagc atttttgatc tttcggtgat catgtctaga aaattctttt ccatacgtat
    19981 cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc aatccgggta gaatcagtac
    20041 gcatgtatgt aatcaagccg actgttcctt gtttacccaa agtgattcct tcatataatt
    20101 gctgtgctac catcatggtt tttcttgttc gaaagtttaa cttacgggca gcttcttgtt
    20161 gcaggctact tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc ttttctactt
    20221 ttttaacttc gaagtctgtt ccatcaattc tagcagtgac ttcttttaca ctttgtgcat
    20281 ctggtaattt ctttttctta ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag
    20341 cttttttaaa attaccgtcg atgctccaat attcttctgg aacaaattct cggatctctt
    20401 tttcacggtc aataataatt tttaaagcga cagattggac acgtcctgca cttaaccctt
    20461 tcttgacctt acgccagaga ataggactga tcgaataacc aacgattctg tctaaggtac
    20521 gacgagcttg ctgtgcatct actaaatcta catcgatcgt tcttggttcc ttaaaagctg
    20581 ctttgactgc gtcttttgtg atttcattga agaccacccg atttttttct ttcagatcca
    20641 acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg
    20701 ccaagtaaac tttttgagct ttttttgctg cggctttcag gcttttgatg acatcgcctt
    20761 ttccgcgtat agaaatataa tggggttcat aattgttttc aaaatcgata cccattttac
    20821 ttttcggtaa atctcttata tggccaacac tggccattac tttatagttt cgtccaagat
    20881 atttctcaat tgtcttggct tttgcaggtg attctacaat cactaaatat ttatacgcca
    20941 ttactgaatg gactcctttc cattgtcgtg ttcctactat taaaagagga ttcatacaaa
    21001 tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg
    21061 tgttattctt taaattctgc caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct
    21121 tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa
    21181 actgttcttc caagatccaa tgcttgctgt gctgttatca gagagccgct ttttttcttg
    21241 gcttcaaaga cacatgttcc tttagacaaa gctgcgataa tccgatttct cattggaaaa
    21301 caatgctttt tcgggccgga ttgaggagga tattcactca aaagaagatg atcatttgca
    21361 atcacctctt gcaagtcacg attttcagca ggatagactt gctctatccc tgttccaatg
    21421 actgcgattg tccgtccacc atgagagatt gctgcttggt gaccgtatgt atctatccct
    21481 ttcgcgcagc cactgacgat cacatatcca gcttctatca ctggttttag taaataataa
    21541 actgtttttt tggctaaagc agaagtgtgt cttccaccaa caacgcttaa catgttacaa
    21601 gaaagtaaag atagatcacc attataaaac agtacaattg gcggataggt caagtgaagc
    21661 agctgagggg gatatttggg atcaaagcaa gtgataaacg actgtttcga ttggattttg
    21721 ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg tttggttatt
    21781 ttacctatct taatgatgtc ttcaaatccg atttctttat aaggatactg catcatccag
    21841 ttagcaagtg cccattttcc ttgactactg attccttttg tataagcaag tttcaataaa
    21901 ttttcttcta tttgatacat cataaaaccg cctttcgtct ctctttctaa taagatacgt
    21961 aaaagcggct gttttttatt gtaaatattt ttttacaggt gcaaaacttt tacgatggat
    22021 tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc tttgttccat aacctgcatt
    22081 atgcgagaaa ccatatccag gataaagcac gtcatattct ttcatcaatt ggtctcgata
    22141 tactttagca atgatacttg cagcagcaat cgacacagat cgtgcatccc ctttgattat
    22201 tttttcctgc tgaataggca aatccaaggt catcgcatcg atcaacaaat agcttggtac
    22261 aatggacaat ttttctactg cttggatcat cgctaattta cttgcttcat aaatattcac
    22321 atcatcgatt gtctgctgat caacgatccc gatcccgatt gccagggctt tttcttgaac
    22381 ttgttgataa agaagttctc gctttttctc tgataattgt ttggagtcat tcagccctaa
    22441 tatgatctca tttttcggca agataactgc agctgccacg acaggtccag ctaatggtcc
    22501 tcttcctact tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc
    22561 aaagcaaaac atttcttcat atttttcctt aattaatgct tcctttttca atcgtcgttc
    22621 aaaagaagcc agtgcttgct gcacgccttt gcgtggatca tttcttaatg attctagtgt
    22681 aggatcatct attctctgta tgcttttcaa tcgttctttg atactttgga tggattcagt
    22741 catcactttt ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca agttttcctt
    22801 gacgaatatc atgcacgact aattcgctgc cacgatcata gtcttcttta tacccacgtt
    22861 ttgctgtaat atccatcagt aattccggtg ccaatttttc ttcttgctcg attgataaac
    22921 cataacgatc gctgattctt cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg
    22981 ctaaatcatc taaatggaca agctggtctt tgatcgcgcc tgtcagcgct agttttttgc
    23041 cgatttcttc atcttcaaat ttgggccata aaatccctgg tgtgtctaac aattccaact
    23101 cattacctaa acgaagccac tgctgacctt ttgttacgcc aggcttattt cctgtctgtg
    23161 caattttctt gcctactagt cgattcatca acgtagattt cccaacattt ggaataccta
    23221 agaccattgc ccgaatagcg cgaggcttga tcccccgagc agcatctctt gcccgttttt
    23281 ctttcaatgc ttctttagcc gcaggtacta gacctttaat tcctttactt tcttgtgccg
    23341 tgatagctaa tgcaagatgg ccttgtttac gaaaatattc ttgccacagc tttgtctgtt
    23401 gtggatctgc cagatccgct ttattcaata aaacaaggcg aggtttttgc tgaatgatct
    23461 gatctagcat tggattacga gaagataaag gtaatcgtgc gtctaccaat tcaaatacga
    23521 tgtccacata tttcattttt tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa
    23581 accattggat ggtcattgtt ctctccattc ctttcatacc ggaatattcc ccggctttac
    23641 ttgttttttt ctttttcaat ataaattcac ttttttatta taaagaaatt ccatctatta
    23701 gttaagtgaa agtgaaccag tcctcattct atcacatctt cactttttt
//
LOCUS       DAZKOM010000035        23657 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000035 DAZKOM010000000
VERSION     DAZKOM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.035"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..767
                     /locus_tag="ACG8NF_001346"
     CDS             66..767
                     /locus_tag="ACG8NF_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0471835.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1376..2392)
                     /gene="tsaD"
                     /locus_tag="ACG8NF_001347"
     CDS             complement(1376..2392)
                     /gene="tsaD"
                     /locus_tag="ACG8NF_001347"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0471836.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2389..2853)
                     /gene="rimI"
                     /locus_tag="ACG8NF_001348"
     CDS             complement(2389..2853)
                     /gene="rimI"
                     /locus_tag="ACG8NF_001348"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0471837.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2860..3402)
                     /gene="rimI"
                     /locus_tag="ACG8NF_001349"
     CDS             complement(2860..3402)
                     /gene="rimI"
                     /locus_tag="ACG8NF_001349"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0471838.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3386..4108)
                     /gene="tsaB"
                     /locus_tag="ACG8NF_001350"
     CDS             complement(3386..4108)
                     /gene="tsaB"
                     /locus_tag="ACG8NF_001350"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0471839.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4299..5279)
                     /locus_tag="ACG8NF_001351"
     CDS             complement(4299..5279)
                     /locus_tag="ACG8NF_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0471840.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5303..6787)
                     /gene="galT"
                     /locus_tag="ACG8NF_001352"
     CDS             complement(5303..6787)
                     /gene="galT"
                     /locus_tag="ACG8NF_001352"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0471841.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6799..7788)
                     /gene="galE"
                     /locus_tag="ACG8NF_001353"
     CDS             complement(6799..7788)
                     /gene="galE"
                     /locus_tag="ACG8NF_001353"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0471842.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8036..8203
                     /locus_tag="ACG8NF_001354"
     CDS             8036..8203
                     /locus_tag="ACG8NF_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0471843.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8265..10076)
                     /locus_tag="ACG8NF_001355"
     CDS             complement(8265..10076)
                     /locus_tag="ACG8NF_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0471844.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10073..10438)
                     /locus_tag="ACG8NF_001356"
     CDS             complement(10073..10438)
                     /locus_tag="ACG8NF_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0471845.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10601..10996)
                     /locus_tag="ACG8NF_001357"
     CDS             complement(10601..10996)
                     /locus_tag="ACG8NF_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0471846.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11014..11976)
                     /locus_tag="ACG8NF_001358"
     CDS             complement(11014..11976)
                     /locus_tag="ACG8NF_001358"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0471847.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11976..12188)
                     /locus_tag="ACG8NF_001359"
     CDS             complement(11976..12188)
                     /locus_tag="ACG8NF_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0471848.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12209..12907)
                     /locus_tag="ACG8NF_001360"
     CDS             complement(12209..12907)
                     /locus_tag="ACG8NF_001360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0471849.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12927..13469)
                     /locus_tag="ACG8NF_001361"
     CDS             complement(12927..13469)
                     /locus_tag="ACG8NF_001361"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0471850.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13601..14605)
                     /locus_tag="ACG8NF_001362"
     CDS             complement(13601..14605)
                     /locus_tag="ACG8NF_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0471851.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14602..15591)
                     /locus_tag="ACG8NF_001363"
     CDS             complement(14602..15591)
                     /locus_tag="ACG8NF_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0471852.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15588..16394)
                     /locus_tag="ACG8NF_001364"
     CDS             complement(15588..16394)
                     /locus_tag="ACG8NF_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471853.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16560..17516
                     /locus_tag="ACG8NF_001365"
     CDS             16560..17516
                     /locus_tag="ACG8NF_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0471854.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17593..18111)
                     /locus_tag="ACG8NF_001366"
     CDS             complement(17593..18111)
                     /locus_tag="ACG8NF_001366"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0471855.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18199..18348)
                     /gene="rpmG"
                     /locus_tag="ACG8NF_001367"
     CDS             complement(18199..18348)
                     /gene="rpmG"
                     /locus_tag="ACG8NF_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0471856.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18576..19022
                     /locus_tag="ACG8NF_001368"
     CDS             18576..19022
                     /locus_tag="ACG8NF_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0471857.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19217..21112)
                     /gene="asnB"
                     /locus_tag="ACG8NF_001369"
     CDS             complement(19217..21112)
                     /gene="asnB"
                     /locus_tag="ACG8NF_001369"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0471858.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(21437..22411)
                     /gene="bsh"
                     /locus_tag="ACG8NF_001370"
     CDS             complement(21437..22411)
                     /gene="bsh"
                     /locus_tag="ACG8NF_001370"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0471859.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(22745..22832)
                     /locus_tag="ACG8NF_001371"
     tRNA            complement(22745..22832)
                     /locus_tag="ACG8NF_001371"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22798..22800),aa:Ser,seq:cga)
     gene            complement(22977..23543)
                     /locus_tag="ACG8NF_001372"
     CDS             complement(22977..23543)
                     /locus_tag="ACG8NF_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0471860.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         7131 a         4989 c         4032 g         7505 t
ORIGIN      
        1 aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact
       61 ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt
      121 ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt
      181 ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga
      241 ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact
      301 atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt
      361 atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa
      421 aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag
      481 ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa
      541 aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca
      601 ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa
      661 ctgctggaca agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc
      721 gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat
      781 aagcagtgac cctgataaat atcagggtca ctgcttattt tcctccctaa aacttctgaa
      841 agatccggca gttttagatc actggacaag actcaaagat tttctatgtg tgtttcagtt
      901 atgtaatatt cactgtttgc aaaaaatact gttagattaa ctagatgaag cgtcttgtcc
      961 tcatccgatc tagattattc gatattcctc gacaataaac gattactcag tgagggcaat
     1021 cagaggaacg gataatgata aataagctgt aaaaaatagc aaaataacaa taatgattaa
     1081 aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca gccctatttc gattaaacgg
     1141 tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt
     1201 tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac
     1261 ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac atgagaagcc
     1321 gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttatt
     1381 cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt cttttttgca
     1441 tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt cgaagagggg
     1501 gaatcactag ttctacctca gggagtttag ttgccaaagc atcctgcaat ccttcacgca
     1561 acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga taattttgac
     1621 aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag ctagctgcta
     1681 aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa ttgataaatg
     1741 cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc gctcgcggaa
     1801 aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg cttgggtaag
     1861 aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca tctcttgtct
     1921 ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta tgtcctccgc
     1981 taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct gcatagatat
     2041 gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca aaagccttag
     2101 ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg acagcgactg
     2161 ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag cataaggtga
     2221 tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa cgtttatgac
     2281 tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta acgaccgcta
     2341 cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc atttcatcgt
     2401 ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc gataatatga
     2461 tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg ctgcttcatt
     2521 agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg taatcagtaa
     2581 ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag caatatttgt
     2641 gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct ggttatcttc
     2701 agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact gcattttcgt
     2761 ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt ctgccaagta
     2821 cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt ctaaaagttt
     2881 tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga tcgttgttgc
     2941 ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac gcacttctaa
     3001 tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa taagcagcga
     3061 accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat ggcaatctaa
     3121 accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga tacctagata
     3181 tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc atggcaattc
     3241 gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg tagtcaattc
     3301 tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa tcattttatc
     3361 ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg tctccaggtg
     3421 tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga ggaagaaaag
     3481 cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt acaccacttg
     3541 gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc tttatttcag
     3601 agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc ttttgtagtt
     3661 gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct aatctatttt
     3721 ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac atgggcacaa
     3781 taatattatc cacaccgatg caattagccg cgatggtttt cagactagat actcctacta
     3841 gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata cgaagccctg
     3901 tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa
     3961 gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg cgtttgatat
     4021 ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc aaagtttggt
     4081 ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg acattctcgc
     4141 cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca aaactagtac
     4201 aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc
     4261 tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga ttctcgaata
     4321 atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt acttaattga
     4381 agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac agtagaaagc
     4441 gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga aatatcttct
     4501 ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta
     4561 gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatactgctt cactgcttga
     4621 tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta gcctttttct
     4681 gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg gtttttcttc
     4741 gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac aaagtgttca
     4801 atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc ttgagaagtg
     4861 ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa taactgagct
     4921 tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat ggaatcccac
     4981 gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc taggcgaata
     5041 gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat caggcgaagc
     5101 gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg ttctgctgct
     5161 tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata gttgagaact
     5221 cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat tgttgccatt
     5281 tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc
     5341 ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa acctgaccta
     5401 cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta
     5461 attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct aataagaaat
     5521 gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc cccattactt
     5581 cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga tgaaaaatcc
     5641 catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac tcaaagtaat
     5701 tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag
     5761 ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact gagttagctg
     5821 cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc ggccatttca
     5881 cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct atcggtgctt
     5941 ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga atcgatcctc
     6001 caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc
     6061 gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa
     6121 tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag ctttcaccgt
     6181 ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag cgacctctat
     6241 atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc gcctcgcgtt
     6301 ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg atttccagtt
     6361 caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt gttttgatgt
     6421 agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg cttttcgaat
     6481 agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt
     6541 gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt tctcttgcct
     6601 tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt acttcaatgc
     6661 tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat aagcggtcca
     6721 tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga
     6781 ttgacatatc tcattcccct attcttcata accatttgga tggctgacat gccaatccca
     6841 tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca gaatctctct
     6901 cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg gtgctatttc
     6961 tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct tgactgagta
     7021 tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca agtattccaa
     7081 agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga tacatgttcc
     7141 atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac caagagctac
     7201 ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga tagatgcatc
     7261 tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc catatgcttg
     7321 gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg gatttttagg
     7381 attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag ttgctgcagt
     7441 agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta agatttgcat
     7501 accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc ctactaaaga
     7561 gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg aacgtagaaa
     7621 ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg cttgtttgtg
     7681 acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta gctggtctac
     7741 agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa aaaacactcc
     7801 tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa gtttactttt
     7861 atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca
     7921 tttagtaaac attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct
     7981 ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa
     8041 aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt
     8101 ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga
     8161 agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat ttaaaaatac
     8221 gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt gtcagtcaat
     8281 ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct
     8341 actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac
     8401 tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct
     8461 gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc
     8521 aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca
     8581 atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt
     8641 tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct
     8701 aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag atgagtaaac
     8761 catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc aatagcctgc
     8821 cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa
     8881 ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc ttctgaaaag
     8941 ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct ataagggatt
     9001 agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa
     9061 tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata aaacaactca
     9121 acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat
     9181 tgctccaggt tttcatttgt tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga
     9241 tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc
     9301 ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc tcctcttgat
     9361 actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt
     9421 tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag ctgatacagt
     9481 aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc
     9541 cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa
     9601 agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa ctgaatagaa
     9661 gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat
     9721 cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt
     9781 ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg
     9841 ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt
     9901 tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca
     9961 ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga
    10021 atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc
    10081 atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg
    10141 caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag
    10201 cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa
    10261 atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca
    10321 accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat ctatccacga
    10381 gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag
    10441 ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag cacaaaataa
    10501 aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat
    10561 gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga
    10621 tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt
    10681 tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa atatagacag
    10741 gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg
    10801 cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt
    10861 ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt ttggccatta
    10921 ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa atgatattaa
    10981 ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc
    11041 agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct
    11101 tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc
    11161 tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat tccccaagtt
    11221 tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc
    11281 gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt tgtctagcat
    11341 tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta ggcgtaccac
    11401 acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc
    11461 ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc cgtgaatcaa
    11521 atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga atacaacgtc
    11581 tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcgtcatt
    11641 atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc
    11701 tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg
    11761 ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg
    11821 attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc
    11881 gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt
    11941 tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt
    12001 tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca
    12061 atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg
    12121 cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag
    12181 ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca
    12241 gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta
    12301 tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa
    12361 ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg
    12421 tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa
    12481 aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta cttgcaccac
    12541 cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga
    12601 acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa
    12661 aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg
    12721 tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat
    12781 aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta
    12841 aaaaagcata ggtccataac ggtcgtctta accatacacg caattttatt tgctgttgat
    12901 aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca
    12961 aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt
    13021 ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg
    13081 gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc
    13141 ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc
    13201 atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt
    13261 tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc
    13321 gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc
    13381 tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc
    13441 cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt
    13501 ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa
    13561 gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt
    13621 tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc
    13681 ctgtaggtac ttccccactt agtagaatca ctctagcaac catatctgca ctaagcagaa
    13741 aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta
    13801 gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc accgctacac
    13861 taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa
    13921 ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa
    13981 gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc
    14041 tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata
    14101 attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca
    14161 ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc
    14221 aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt
    14281 gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata
    14341 tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa
    14401 gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca
    14461 gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa
    14521 aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat
    14581 agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg
    14641 aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct
    14701 cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac
    14761 ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa
    14821 tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac
    14881 aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg
    14941 aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg
    15001 aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc
    15061 aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga
    15121 actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg
    15181 caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac
    15241 agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc
    15301 cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa
    15361 tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg
    15421 aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca
    15481 aaaatctgct tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa
    15541 aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc
    15601 tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc
    15661 gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct
    15721 tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa
    15781 cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg
    15841 atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt
    15901 tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg
    15961 gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca
    16021 tccaacgccc actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga
    16081 taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta
    16141 gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt agagatatca
    16201 gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat
    16261 aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc
    16321 gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct
    16381 cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt
    16441 tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt
    16501 cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa
    16561 tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga
    16621 gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat
    16681 ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc
    16741 ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta
    16801 tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg
    16861 atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt
    16921 tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa
    16981 tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag
    17041 atgtcggcaa agctttggca aaagaaaaag aggcagaaaa gatcatttcc gattatcaaa
    17101 aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag
    17161 tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa
    17221 tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag
    17281 ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca
    17341 tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc
    17401 ttaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata
    17461 acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt
    17521 aactaaaaaa agcatcttca cgattagtcg tgatatgctt tttttcttat taacaaacta
    17581 gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg
    17641 aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc
    17701 gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa
    17761 tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga
    17821 aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat
    17881 tacttccgca ttttctttcg tgcgaaaatt tggctgcgtt gtcaacacga tgatatggcg
    17941 ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg
    18001 atccattgta tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc
    18061 atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc
    18121 tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa
    18181 ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg
    18241 gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata
    18301 gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc
    18361 catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt
    18421 ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac
    18481 ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat
    18541 atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc
    18601 ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg
    18661 cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa
    18721 aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc
    18781 caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg
    18841 tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa
    18901 gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca
    18961 aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt
    19021 gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa
    19081 tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg
    19141 ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat
    19201 tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt
    19261 catctacaaa gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt
    19321 gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa
    19381 aaaattcttt tgcaaaatct gcttcgaaca atgctttgac ttgttcgtag ccgtgttctt
    19441 ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc gaccattttt
    19501 ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata
    19561 aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc
    19621 ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca
    19681 tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt
    19741 tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta
    19801 acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg
    19861 caggagcaag tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt
    19921 tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac
    19981 ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg
    20041 ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca
    20101 aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg
    20161 ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg
    20221 ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag
    20281 aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag
    20341 aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga
    20401 ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat
    20461 attgctgaat gtctattttt ccatctttat acgtataata atggccttca ggcaaacgat
    20521 agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt
    20581 tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt
    20641 ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt
    20701 ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc
    20761 cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg
    20821 tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt
    20881 aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct
    20941 gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat
    21001 cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa
    21061 taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt
    21121 cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat
    21181 agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc
    21241 tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta
    21301 ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa
    21361 aaaagacacg acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa
    21421 cggatctatt cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac
    21481 tgaccaattc atgacgatcc aagtcttctt tattcatatt tacagcagta atttgactgt
    21541 cttcatacgt acgataataa taaatccctt tatcaacgtt acagcaagaa gaataaatcg
    21601 tatattcgta ttttctttct ccagtatcgc atactccttt ttgttgctcg actgagccta
    21661 gtatatgaaa aaattgactg atactttcag attctgaatc acctgatgca gcattcattt
    21721 tagtaaatgt ggcttttaca aatcgagaaa cagaagagag atctccaggt aagcccaatc
    21781 ctcccatccc acgactataa acattcaagc tgatctggtt tgaaaaatga ttttctggtg
    21841 ttccatttga tagcgagcga taattgttca gattaaataa ttgataatca aatggcggat
    21901 tatttgtcaa cacaccgaca ggattatcat ataaatgaag tccatctttc atactttcaa
    21961 ttactatcga tttttcttta tcagctaata accagtgtaa aggtgacaag ggtagttttt
    22021 cactaaaatc gatattgact agattgatag tagccaataa ttctttagct tcgtctatgg
    22081 ttgagcattg ccctagtatc catggaataa actcaaatgg tgcaacgtta tcttttcctt
    22141 cttgtaattc tttataatca gcattgccag gaaaattcag tcctgccata ctcaaccctt
    22201 tttcatttgt agcctcataa tagagtggat agttggatac cccagctgct attcctatca
    22261 ttgcataatg tttatctaaa ttttttacct ttctaaaatc gaaacgataa tttcttggag
    22321 taatagtgac cacttcatta taagacatct cataatcaaa attccttcca aaataatggt
    22381 ctttcgttgc ataagtgatt gcggtacaca tcgtttcccc tcctaaaaca aatcgttttt
    22441 acacttttat cttacatcta gtattttttt tatcaaaaaa aggccaaaga agattgattt
    22501 actagcattt ataaaaaata gtactcaggg ggaatcaaaa aatattgtag gttaaaagta
    22561 caacaataag gggctgtgac aaaagtcttg tcacagcccc ttattccgaa caaacggtgt
    22621 tcaaaagctt gcatctgcgg taataagccc aatcaaacga aaaaatagga aaagctattt
    22681 ttgtttggcc gttcttatta cttgatgcag gacagcttct tcacaacctc taatctttga
    22741 tataaggaag gtacaggatt tgaacctgcg cgccggtatt agccggttcg ccggatttcg
    22801 agtccggtgc attaccactc tgccaacctt ccgtaagtac tatttttaaa cttttttcac
    22861 gaaatgtcaa gcctatttct aatagataaa taacagaaat cgcttttttt agcaaaaaag
    22921 tccattgaca gtttgcagcc aacggactta ccgatcaaat tcatactgat agaacactat
    22981 ctgattattt caggattatc taatgctaaa atcgagtcaa ttgtccgttt gatcagtgca
    23041 atgatttgtt cttctgttgg atgcatgatg tttgatgaca tcaatgacgc taagccagtc
    23101 actgctaccc atgtaccagt atgcagagca tcgacatatt catccgataa ttgattgtat
    23161 ctttcatctt ctttaactat ttcacggaaa taattgtatg aaaaatcatg cattttcttt
    23221 ccgtccccat gttcatcaat gtacaatgcc atgtaaagac tatgatgctt ttttgcaaag
    23281 tagatatagt tcaaaccgag atcaatgatt ttatcaccag tacgttcaat agggaaaaca
    23341 tcttctttta gttgttcaaa gatagaatcc agcaacgtat tttttaaatc ttccatgttt
    23401 ttgaattcca gataaatcgg ctgtgtcgaa atccccatct ttttagcaat atttctcgca
    23461 gtaaatcctt taaacccttc ctttgcaaca acatcataag cagcttttag aatttgatcc
    23521 ttggtataga cttttcttct cattaaaact cccccacttc tttactgcag tatatgttgt
    23581 tttattgtac cctattttta gcattttttg caaacatttt actcaaaacg ttatatttta
    23641 aatttttttt cagtctt
//
LOCUS       DAZKOM010000036        23642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000036 DAZKOM010000000
VERSION     DAZKOM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.036"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8NF_001373"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8NF_001373"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0471861.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG8NF_001374"
     CDS             complement(1171..1866)
                     /locus_tag="ACG8NF_001374"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0471862.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG8NF_001375"
     CDS             complement(1879..2595)
                     /locus_tag="ACG8NF_001375"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0471863.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG8NF_001376"
     CDS             2925..3533
                     /locus_tag="ACG8NF_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0471864.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8NF_001377"
     CDS             3858..4181
                     /locus_tag="ACG8NF_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0471865.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8NF_001378"
     CDS             4363..5733
                     /locus_tag="ACG8NF_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0471866.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8NF_001379"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8NF_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0471867.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG8NF_001380"
     CDS             complement(7518..9509)
                     /locus_tag="ACG8NF_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0471868.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG8NF_001381"
     CDS             complement(9653..9919)
                     /locus_tag="ACG8NF_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0471869.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8NF_001382"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8NF_001382"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0471870.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8NF_001383"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8NF_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0471871.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG8NF_001384"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG8NF_001384"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0471872.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG8NF_001385"
     CDS             complement(14079..14600)
                     /locus_tag="ACG8NF_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0471873.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG8NF_001386"
     CDS             complement(14603..15445)
                     /locus_tag="ACG8NF_001386"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0471874.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG8NF_001387"
     CDS             complement(15438..16163)
                     /locus_tag="ACG8NF_001387"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0471875.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG8NF_001388"
     CDS             16339..16635
                     /locus_tag="ACG8NF_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471876.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG8NF_001389"
     CDS             17203..18795
                     /locus_tag="ACG8NF_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0471877.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG8NF_001390"
     CDS             18808..19548
                     /locus_tag="ACG8NF_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471878.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG8NF_001391"
     CDS             19716..21065
                     /locus_tag="ACG8NF_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0471879.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG8NF_001392"
     CDS             complement(21193..21783)
                     /locus_tag="ACG8NF_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0471880.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8NF_001393"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8NF_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0471881.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4761 c         4502 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aa
//
LOCUS       DAZKOM010000037        23519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000037 DAZKOM010000000
VERSION     DAZKOM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.037"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1804
                     /locus_tag="ACG8NF_001394"
     CDS             143..1804
                     /locus_tag="ACG8NF_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0471882.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1874..2221)
                     /locus_tag="ACG8NF_001395"
     CDS             complement(1874..2221)
                     /locus_tag="ACG8NF_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0471883.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2305..3246
                     /gene="opp3b"
                     /locus_tag="ACG8NF_001396"
     CDS             2305..3246
                     /gene="opp3b"
                     /locus_tag="ACG8NF_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0471884.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3247..4290
                     /locus_tag="ACG8NF_001397"
     CDS             3247..4290
                     /locus_tag="ACG8NF_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0471885.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4296..5366
                     /locus_tag="ACG8NF_001398"
     CDS             4296..5366
                     /locus_tag="ACG8NF_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471886.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5356..6309
                     /locus_tag="ACG8NF_001399"
     CDS             5356..6309
                     /locus_tag="ACG8NF_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471887.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6321..7331
                     /locus_tag="ACG8NF_001400"
     CDS             6321..7331
                     /locus_tag="ACG8NF_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0471888.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7616..8437)
                     /locus_tag="ACG8NF_001401"
     CDS             complement(7616..8437)
                     /locus_tag="ACG8NF_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0471889.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8534..8812)
                     /locus_tag="ACG8NF_001402"
     CDS             complement(8534..8812)
                     /locus_tag="ACG8NF_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471890.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9080..11089
                     /gene="metG"
                     /locus_tag="ACG8NF_001403"
     CDS             9080..11089
                     /gene="metG"
                     /locus_tag="ACG8NF_001403"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0471891.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11568..12155)
                     /locus_tag="ACG8NF_001404"
     CDS             complement(11568..12155)
                     /locus_tag="ACG8NF_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471892.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12330..13652
                     /locus_tag="ACG8NF_001405"
     CDS             12330..13652
                     /locus_tag="ACG8NF_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0471893.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13807..14583
                     /locus_tag="ACG8NF_001406"
     CDS             13807..14583
                     /locus_tag="ACG8NF_001406"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0471894.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14576..15145
                     /gene="rnmV"
                     /locus_tag="ACG8NF_001407"
     CDS             14576..15145
                     /gene="rnmV"
                     /locus_tag="ACG8NF_001407"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0471895.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15138..16022
                     /gene="rsmA"
                     /locus_tag="ACG8NF_001408"
     CDS             15138..16022
                     /gene="rsmA"
                     /locus_tag="ACG8NF_001408"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0471896.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16473..17102
                     /locus_tag="ACG8NF_001409"
     CDS             16473..17102
                     /locus_tag="ACG8NF_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471897.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17226..18335)
                     /locus_tag="ACG8NF_001410"
     CDS             complement(17226..18335)
                     /locus_tag="ACG8NF_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471898.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18544..18966
                     /gene="mgsA"
                     /locus_tag="ACG8NF_001411"
     CDS             18544..18966
                     /gene="mgsA"
                     /locus_tag="ACG8NF_001411"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0471899.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19153..19251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19289..19825
                     /locus_tag="ACG8NF_001412"
     CDS             19289..19825
                     /locus_tag="ACG8NF_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0471900.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19865..21628)
                     /locus_tag="ACG8NF_001413"
     CDS             complement(19865..21628)
                     /locus_tag="ACG8NF_001413"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471901.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21631..23346)
                     /locus_tag="ACG8NF_001414"
     CDS             complement(21631..23346)
                     /locus_tag="ACG8NF_001414"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0471902.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7590 a         4301 c         4778 g         6850 t
ORIGIN      
        1 tccttgaaac ctcacgaaaa aatcattatt ttatgaaaaa gacaaaaaaa agtgttgatt
       61 ttttaataat tattcattaa actcttaaac aacttcagaa aatattaatt attctgaaaa
      121 ttccaataaa ggggtacgta aaatgaaaaa gaaatttaca tttggtttgg ttgcggtatg
      181 cggccttgta cttgctggat gttatggcgg cagttcagcc acaagcagta gttcagcaaa
      241 aagcggttca gcagatggag gaggcgtatt taacttagtc gtgcctcaag aaatgcctac
      301 agctgactta tcagttgcaa cagatacaat cagttttaca gcattgaata acgtttatga
      361 aggaatttac cgtcttgatg aagacagcaa accacagccg gcaggagcta gcgagttggc
      421 agaagtcagt gaagacggat taacttacaa aatcaaatta cgagaagacg caaaatggtc
      481 aaatggtgat cctgttacag cagcggatta cgtttatggc tggcaacgaa cagtagaccc
      541 agctacagct tctgaatatg cttacctgta tgcgccagtt gaaaatgcag aagacattac
      601 agctggaaag aaagataaat cagaactagg gatcaaagca gttggtgact atgaattaga
      661 aatcaaattg acgaaacaaa caccatattt ccaatatctg cttgctttcc catcattttt
      721 cccacaaaaa caatccgtag tggaagaaaa tggggatgcc tatgcatctg caagtgataa
      781 agctgtttat aatggaccat tcacattaga agattttgat ggaccaggaa cagatacaga
      841 atggacttat aagaaaaatg atcattactg ggacaaagat acagtaaaac tttctgaaat
      901 caaagtcagt gtagtaaaag aatcttctac tgcattgaac ttattcaaag atggccaagc
      961 agatgacgtg atcttgtctg gtgagttagc acagcaaaac gcaaacgatc cagcttatac
     1021 ttctgttaaa gaagcgcgga caagctatat cgaattcaat caacgtgaaa agaattcacc
     1081 attcaacaat gtgaacctaa gaaaagctat ttcttattct atcgatcgtg aagctttagt
     1141 aaaacaagta ctaggtgacg gatcagttgt ctctacagga ttgatcccat cagatatggt
     1201 aaaaagtcca acaacgaacg aagactttgc aaaagaagct ggaaaattag tttcttatga
     1261 caaagataaa gcaaaagaat attgggaaaa agcgaaaaaa gaattgggcg ttgattcttt
     1321 agaatttgat ttgatggctt ctgatgatga ttcatcgaaa aaagtaatcg aatatgttca
     1381 aaactcaatc caagaaaact tggatggcgt aacagtcaaa ccaacacctg ttccattctc
     1441 tgtacgtctt gaccgctcaa cttcgggtga ttttgacgca gtactaggcg gatgggctgc
     1501 tgactatgca gacccaagca gctttactga tttgtttgtc acaggtaatt catacaaccg
     1561 tggtcgctgg agcaatgcag aatatgacga agcagttaaa gcagcttcat caacagacgc
     1621 tggcgatgaa caagcccgtt gggatgattt ccaaaaagca atcaagatca tctctgatga
     1681 tatgggtgtc gttccggtgt accaaaaagc agaaggtcac ctagtgaatg aaaaaatcaa
     1741 aggtatcgtt caccatgcgg caggcgcttc atgggattac aaatggacat acgttgaaga
     1801 ataatcgtta ataccgtgag catgagaaaa aacgaggctg cggcaaatgt cgcaacctcg
     1861 ttctcatatt cacttatcct aacaaagaaa aaagcatgaa aatagcagaa agagcaatta
     1921 gtattccggc aatgatcagc caaaagctgt acataccgta gctggcagac gagagcgagg
     1981 aaaattctgg ttttttcttt cggttccttg ttcgtgcaag atggaccgat cgctgaaaat
     2041 gatcgaagaa acccgaagaa aagacccata aaaaaccgcc gataatcaga aacggcaatg
     2101 accataagaa tagatcatta gataattgaa tgagtgtaaa ggaatggttg atgagatttt
     2161 ttattaaaat aaccagaatc agtagaaaag ctactgcgta ggggatagat ttttttttca
     2221 tttgattgct cctttatttt tggttcatta tctaataatg tagcgaaaca tctgtcagaa
     2281 gtcaaaagct agggggaaac attagtgaat agttatatca aatatgtatt aaaacgtgtc
     2341 tttttcatga tcatcacgct ttggttaatt gcaacaatca cctttttctt gatgcaattg
     2401 ctgccgggca cgccatatac gaatcaggaa cgtttgagtc ctgaaacaat cgagatgcta
     2461 aataaacaag tcggtttgga taaaccagtt atcgtacaat atgggattta tctttctaat
     2521 ttgcttcaag gagattttgg aatttccttc caatttaaaa accagccagt cgcacatttg
     2581 ttagctggaa gaatcggacc atccttgcag ttagggttac aggcaattat ctttggaaca
     2641 gtcctaggga cgattttagg aacgatctct gccatgaagc aaaatacatg ggcagataca
     2701 tcttctacac tcgtagcgat attaggtcgt tccattccaa actttgtatt tgctgtttta
     2761 ttacaatata tctttgcaat caaattacaa gttttgccaa tcgcaaaatg ggatggcttt
     2821 gtgtacacaa tcttaccaac aattgcgttg gccatgtcgc cgcttgctga ctctgctcgg
     2881 tttatccgta cggaaatggt ggaagtactg catagtgatt atgtggaatt agcaaaagca
     2941 aaaggattga gccgttggga aatcgccttt aaacatggtc ttcgaaatag tttgattcca
     3001 ttgatgactt tattaggacc gttagctgta gcattgatga ccggatcact ggtggtagaa
     3061 aatatctttg ctattcctgg tatcggggaa caattcgtta agtcaatcac aacaaatgat
     3121 tatcctacga tcatggctgt aacgatcctt tactcattta tgctgatttt tgttattttg
     3181 atcgtggatc tattgtacgg tctagttgat ccaagaattc gtgtttcaga agggagccga
     3241 ggataaatgg aaatgatacc aaagcgttat gaaacagttg cagatatccc tgcggatgaa
     3301 tttcttccat tacaaaaaaa taccgaagaa gaacgtgaac agattactgc accttctttg
     3361 aattttatcc aagattcatg gcgccgttta aagaaaaaca aagctgccgt tatttcaatg
     3421 gttttactat tggtcatcat tttgatctct gtgatcacaa tctttgtttc tcctcatgat
     3481 ccaactgccc aaaacgtggc ttatatcaat ttgcctcctc gtattccggg aatcaatatc
     3541 gatggattga atggaaaagc aatggttggt ggtgaacttg ttgataaata tgctcaagca
     3601 aacgtccctt caaacgtaaa tttcttttta ggaacagacg gtttgggacg tgatgtatta
     3661 agccgcttat ttatgggaat gagaatctcg ttgctgatcg catttattgc tgcattacta
     3721 gatgtaacga ttggtgtagc ttacgggttg gtttctggtt tgttaggcgg acgtgtcgat
     3781 aacgtgatgc aacgtttttt ggaaatctta tcaggaattc caaacctcgt tgtcatgatt
     3841 ttgatgttgg tcgtttttga accaggaatt ttttcaatcg tggctgctat ggccatcaca
     3901 aactggatac caatggcacg tatcgttcgt gcacaaacaa tgaaattaaa agatcaagaa
     3961 tatgttttag ctggattgac attaggtgaa tcaagaatga aaattgcatt caaacatatt
     4021 ttgccgaata tctctagtgt cattattatt caaatgatgt ttagtattcc atctgctatt
     4081 ttcttcgaag cctttttaag ctttatcggt ttaggtctta cgcctccgtc agcttcatta
     4141 ggaacgatgt tgagcgaagg gtacaagaca ttcctgtatc tgccttatct attatggatt
     4201 ccggcagtaa ctttatcggt tatcatgatt ggctttaact tacttgctga tggattgcgt
     4261 gatgcctttg atcctaaaat gaaagagtga agacgatgac gaaaatttta gaagtgaaag
     4321 acttagaaat ctcttttgat acgtatgctg gaaaggtacg agcgattcgc ggtgtcaatt
     4381 ttcatttgaa taaaggagaa acattggcga tcgttgggga atctggaagt ggaaaatcag
     4441 tgacgaccag aagcatcatg cgcttgctgt caagcaacgc caatatagat gctggtcaaa
     4501 tcctctttaa aggtaacgat atcgttggaa aatcagaaag agagatgcaa aaaatccgtg
     4561 gtaaagaaat agcgatgatt ttccaagatc caatgacctc tttagatccg acgatgccca
     4621 ttgggaagca agtcgctgag tcccttagaa agcataataa agtttcaaaa aaagagggac
     4681 aaaaagctgc attagaattg cttaaattag ttggaatacc cgaagctgag aaacgtatca
     4741 acagttatcc gcatcagttc tcaggaggtc agcgtcaacg gatcgtgatc gcgattgcac
     4801 tggtatgtta tccggaaata ctgattgctg atgaacctac aacagcgctt gatgtgacta
     4861 ttcaagcaca gattttggag ctgctgaaag aaattcagac aaaaatcgat acatcaatta
     4921 tctttatcac tcatgacctt ggtgtagtag cgaatgtcgc tgatcgtgtc gctgtcatgt
     4981 atggaggaag aatcgttgaa gttggaacat cggaagaaat tttctacaac ccgcaacatc
     5041 cctatacttg gggactgctt ggttcaatgc cgacattaga tagtgcgaac gatcgattgt
     5101 atgctattcc tggttctcca ccggatttat tgaacccgcc aaaaggagac gcattctatc
     5161 caagaaatga attcgcgatg aaaatcgatg cagaacaaga accaccattt tttgaactat
     5221 caaaaacaca caaggcagca acttggctac ttgctccgca ggcacccaaa gtgacaccgc
     5281 cagaagaaat ccaacgtcgc tgggctatct ttaaagagaa acaaaaacat acttacggtc
     5341 aaggaggaat tgctgatgcc aaataaacta cttgaagtta aaggattgaa acagtatttc
     5401 aatgtaggaa aaaagaatga agtacatgct gtaaatgaca tcagctttca catttatgaa
     5461 ggagaaacat tcggtttagt tggagaatct ggaagcggaa aatccactac tggccgtacg
     5521 atcattcgct tgaatgaacc tactgatggt gagatattat tcgacggtca agatataacg
     5581 aagatcaaag ataaaaaagg tttgacgaaa ttccggcatg atgttcagat gatctttcaa
     5641 gatccatacg cttcgttgaa tcctcgtatg aaagtcagag atatcattgc cgaaggaatt
     5701 gatgttaatg gactagctaa atcatctgaa gagcgtgcaa aaaaagtcga tgatctattg
     5761 agaactgttg gattgaatcc aagccacggt actcgttatc ctcatgaatt ttcaggtggg
     5821 caacgccagc gtattggtat tgcacgagca ttggcagtaa aaccgcgttt tatcatttgt
     5881 gatgaaccaa tttctgcact agatgtgtcg atccaagccc aagtggtcaa tttgttgcaa
     5941 gatctccaaa aagaacatca attgacctat ctatttattg cacatgattt gtcgatggtt
     6001 aaacatatta gtgaccgaat cggtgtaatg cataacggct tgttattaga gatgggaaca
     6061 agtgatgaaa tctataatca cggtgttcat ccttatacag aaagtttgtt atctgctatt
     6121 cctctaccag atccagacca tgagagaaaa cgcagaagaa tcaaatatca acctgaacca
     6181 gatgacggac aagtacgtca acttcgtgaa atcgcaccag aacattttgt ctatgcaact
     6241 gagcaggaag tcccatatta cgcaaaaaaa ctaaaacgac aaaaggaatc tcttctggtt
     6301 gctaactagg agggacatag atgctaaaag cttttaaatt ccgtatgtat ccgacagaag
     6361 aacaaaaaca gcaattgatt cgtacttttg gatgtgctcg atttacctat aaccatttgc
     6421 tgaaaaagcg gcagaaaagt tggcaacaaa caggagtagc caatttttcg ctcacacccg
     6481 ctacattaaa aaaagaatac ccattcttaa aagaagtaga cagtcttgct ttagcaaatg
     6541 cacagcttaa tttggatcgg gcttttcgca attatttcaa gggacgtgcc agcttcccaa
     6601 agcttaaaac gaaaaaaagt atgtggcaat cttatacgac caacaaccaa acccgtacga
     6661 tctatctgga aaatggctac ttaaagttac caaaacaaaa agaactaatc aagataaatc
     6721 agcatcggcc agttgaagga tcgattcgct ccgctacgat ttctgctcga tataatgaag
     6781 aattttatgt cgcactactt tgtgatgttt cgccagtaaa aaaagaatct ttagctaaat
     6841 ggatcgggat cgcctatcat ccgaagacat tgatccaaac atctcgacct ctagaagtaa
     6901 ctttaccaaa atttcaccag acagaagaaa agctgcaaca tgcacagcgg aaattgaatg
     6961 tgaaagtccg ctccgctcac catagaaaga tcaggttaga tcaggcaagc aattatcaga
     7021 aacaaaagag aaaagtaatg gatctttatc tgaaacaaaa gaaccagcga gaagattatc
     7081 ttgaacaatt atctgggaaa ttggtcaaac aatatgacta cttatttgta gaatcttttc
     7141 caaaagaaga ggcacatgct gatttttcta ttcacgactg gcataaatta ataacaaaat
     7201 tgcgttataa gtcccaatgg tataataaaa aatttctttt gatcaatacc gacggagcag
     7261 aagaaagcaa ttccgtaaga aagagccaag tagtagaaaa aatgggcaga cattcggtta
     7321 tcaagggata aaaaatgaat gaaactgata ggagcgttag ggttagcctt gttaataaaa
     7381 agtcttaatg acttttgttt ctaggaattt ttcttttgaa gaaaagctca ctaattatga
     7441 caaaccaata aaacaatagc agaaaaagtt cgtacgagct ttttctgcta ttgttaattt
     7501 tttattcaga aagtttacga atacatcaaa attctgaaac aaaaaacgta actcacaaag
     7561 agttgttttt gcgttttttc ttgaatgatt aggtctgaat cttttgctca caaccttatt
     7621 catggtgccc cggcttgagc tgatagattt ttgaaggacg gccgattcct acaggacgtt
     7681 caccgatctc atcgaaatat tggagcatag ctttcttaaa attggaatga tcgatcgttt
     7741 tataatcgat ccctaagaat ttagcaaata cttttcgagc ttccgtaatc gtgaaatctt
     7801 tacctaaaac ttgtagaact tgcggctcgt gttccatttt attgactaca cggttgaatg
     7861 ctttcaagat gatttgacta tggtcaaaag ccaaagtatc tttgccagaa gactctccag
     7921 tgactaaatc aagcgagata gccacttctc cgctagataa atggatcttg ttactatgac
     7981 gttctagtgt gaaccagcgt acttccttgg catcatctcc ggcgattagc ggttcttctc
     8041 caataaaggc taagtagctg actgtgacga cccagcctcg cggatctcga ttaggcgtac
     8101 tgaatgtatg cagttgctca atattttctt ttgaaataac cacaccagtt tcttcctttg
     8161 tttctcttaa gacgctttct ccagttgact catccttttg gacaaagcca ccaggcaatg
     8221 cccatgaatt tcgataagga tgcccttttc tttggatcaa caaaactttc agctgatctt
     8281 cttctttgtt atagcacatc agtacgatat cgacagtcaa agagggcttg tcatattctg
     8341 gacgttcctg tttttgatac cagttaagaa attcttcttc acttgcttta tgttcataat
     8401 ataatttttc ttcttcttta gaagcaaaac ggttcatctg accacctcca ttgacagtaa
     8461 aagtactttt taccttattg tagcagactc tgaaaaaaaa ccaaggattt acttgaaaaa
     8521 tctaggttat catttaaata tgaaaaataa atggaagtat gagattgaat aaaaagaaaa
     8581 gcagataaaa cggcaatact ttataccgct cttcacgaat ggcaaagaag ccacaaataa
     8641 cgatatatac gtcaatgata aaacgcaaaa tccaagctgc tgtcataaaa ctgttcaaat
     8701 gcccatggag gttaaaataa ccatacatac gaatataaaa atagctgaat attaaatgtg
     8761 ctaatgaaag aaaagcaata ctaagtgtaa aaaaaagatg tttcttctgc atgattgttc
     8821 cagtccctta ctaagataga tagtctttat tataccacgt gtgaggggag aaataaaaag
     8881 ccgtttttta gtagatatga aacaaaacaa aaacccgcat ttattcaacc tattcatcct
     8941 tttaaaggat agattgaacg tagatagaag tcgtttatcc agtctttttc attttgaaaa
     9001 aaagctaaca gcatgctttt tttatggtat actagcaagg aaacgaattc gcatcatgcg
     9061 ataaaggagg aagagccccg tggcagaaaa ggaaactttt tatatcacta cgccaatcta
     9121 ttatccaagc ggcaaacttc atatcggcaa ctcgtatact acgattgcat gtgacgcaat
     9181 tgctcgttat aaacggttga tgggctttga tgtgttttat ctcactggag tggatgaaca
     9241 tggtcaaaaa atcgaaacaa aagcagaaga actaggtgta aaaccgcaag aatatgtaga
     9301 taaaatggcg gctgatgtca agaaattatg gaaaacgctt gatatcagtt atgataaatt
     9361 cattcgcacg acagatgatt accataaagc agctgttcaa aaaatatttg atcgtttatt
     9421 agaacaggga gatatttact taggtgaata tgaaggctgg tactctgttt ctgacgaaga
     9481 attctttaca gaaacacaac ttgcagaagt gtacaaagat gacgaaggta acgtgatcgg
     9541 tggtaaagcg ccaagtggac acgaagtaga attggtaaaa gaagaatcat acttcttccg
     9601 catgagcaaa tacgcagacc gtctattgga atactatgaa gaacatcccg aattcatcca
     9661 gccagaatct cgtaaaaacg aaatgatcaa caactttatc aaaccaggat tagaggatct
     9721 tgcagtttca cgaacaactt ttacttgggg agtaccagtc aagagtgatc cgaaacatgt
     9781 ggtgtatgta tggatcgatg cattatccaa ttatattact gcactaggat atggctccga
     9841 agatgaaagt ctattcgaaa aatactggcc tgcagatgtt cagatggtcg gtaaagaaat
     9901 cgttcgcttt catacgatct attggccaat catgctgatg gcccttgatt tgcctctacc
     9961 taaaaaagta tttgggcatg gctggctatt gatgaaagac ggaaaaatgt ctaaatcaaa
    10021 aggaaatgtc gtttatcctg aaatgcttgt ggaacgttat ggtttagatg cactccgtta
    10081 ttatttgctt cgtgctgttc cgtttgggtc agatggtgtc tttacgccag aagattttgt
    10141 ttcccgtgtc aattatgatc tggcaaatga tctagggaac ttattgaatc gaacgattgc
    10201 tatgatcaac aaatattgtg atggactcgt tcctgactat gcctcacttg tcactccatt
    10261 tgatagtgaa ctgtctacga cggctgctaa cgttgtcgga cgttaccatg atgcaatgga
    10321 aaaaatggaa ttcaatacag cattggctga aatctgggtg ctgatttctc gtgcaaacaa
    10381 atacattgat gaaacagaac catgggtatt agcaaaagac gaagaaaaga aagctgaatt
    10441 gaatagtgtc atgatccatt tagctgaatc tttgcgtatc gcagcgatat tacttcagcc
    10501 aatcatgaca gaaacgccag agaagatctt caatcaacta ggattggact ctgaaacaat
    10561 gaatcttgaa ggattacact ttggcgaatt tccttcagga acaaaagtag ttgcaaaagg
    10621 tacaccaatc ttcccacgtt tagatatgga agaagaagtt gcttttattc aagaaaaaat
    10681 gtctgaaggt actcaaacaa acgaagatac agtcaaatgg gatccagaag aaacagaact
    10741 tgtttcaacg aaagaaaaac aaatcaaatt tgatgtattt gaaaaagtag aactaaaagt
    10801 agcagaagtc atcaactgtc aaaaagtaga aggggcagat aagttgctgc aattccgact
    10861 agatgctgga gacagccaag accgtcaaat cttgtcagga atcgctgaat tttatcctga
    10921 tccaagcgaa ttgatcggga aaaaagtcgt cattgtagca aatctgaaac cacgaaagat
    10981 gcgtggacaa atcagtcaag gaatgatcct ttctgcagaa gcaccagatg gctctttaca
    11041 agtaatcgaa gcaccaaaat ccatgccaaa tggctcagaa atcgcataga aattcaaagg
    11101 gggtatgaca ctagttgtgt cataccccct ttttccgaaa tttgagaagc aattttcgta
    11161 aattccttct tattacttga agccaatcat ataatacaga aacagagaac tatccaaaat
    11221 aaatcctaga aaaagaagta cgagcttcgc tgatttttgt taatttagaa taatactaat
    11281 aggaatcgta aaaacagaaa aaaagcataa tgataaaggg tcgggcagat tttaattgat
    11341 tcatctggta ctctttttta ttgtccttac attcataaag tttgaatgca atgactttat
    11401 tacatctata tttatatcta acagtagcgt tgatgtgtta attataagtt ctatcacttt
    11461 atgttcaatt agtttgtgct cttatatgtg tgattgaaaa tatagataaa aaaacaatca
    11521 tcggctttga cttcgatgat tgttttcttt attttgtaaa aatcctatca cttgatttta
    11581 tggtaacctt ttatttttgt cttcaaacgg cttggtaaga gagcagtaag cagtgcaaat
    11641 gggatcgaaa agacatacca gaaaatcgta cgtccgatac cataatgttg atagagcttt
    11701 gtcaacatca cgtgtaacaa gatcaaccct aaaagcaagg agccagctat aaaccaatga
    11761 ttatcccaat gcatgacttc actaagtgcc atcactccaa aaggatagaa ataaaaatag
    11821 atcccgacta aggtcatccg ccagttcttt gtaaatgttc cgatattcaa taaaaagaaa
    11881 atcgctgaaa gcaaaatacc gataggcgga aaaagcccaa ccaaaaagga atacaacatc
    11941 ccccacaaga tcatcacagg acctttgatc aatgcaacga ttgcgatgaa caaagtcagc
    12001 aatattagtt tttgtgataa cgttgcaaca gtcattaatc caaataaaat taatgtgaac
    12061 caatgccaat gggactgctg ttgttcttta cgggaaaaat cgacccggtt ggataaataa
    12121 tctgctaatt tcggataaga acgcttaaaa tccataaact gttcctcctt cgtggaaaag
    12181 gtaggtttag tatacgaaaa ataaaaaaga aatacatccg tcttaagagg gagtttttca
    12241 ctgaaagtgg agaaaaaaga atggtaaatt aaaatattct cagttatact gaaaattata
    12301 ccaaatttca aagaagaaag aggatgggaa tgaaaggcaa actaaaacga gaaatcaatc
    12361 tgtttggcgc attagctacc gttatgggaa ccgtgatcgg tgcaggcgtc ttcttcaaaa
    12421 ctgctgctgt tactgctagt actcagtcta caagtttgac attgctagct tggcttttgg
    12481 gtggtttctt aacgatctgt gctggattga cagtggctga attagctaca gcaatcccgg
    12541 aaactggtgg agctgtaaaa tatattgaag ctgcgtatgg aaaattgccc agctttttat
    12601 taggatgggc gcaaagcttg atttactttc ctgctaatat tgctgcatta tcgattatct
    12661 ttgcgacaca attgacgaac ttattacaat tatctaccga ttatcttttg ttgattgcag
    12721 tgatcaccgc tgtttctgtg acagggttga atttgttagg gacaaaagtc ggtgcttctg
    12781 tacaatcagt gacgttgatt gttaaattaa taccaattgc tgtgattgtc atatggggat
    12841 tattgacccc aggacaagga actgtccagt tatttccaat cgaagctgga aaagatgtta
    12901 cttttgtcga aggattgagc agtgcgttgt tagcaacgtt gtttgcttat gacggctggt
    12961 taggtgtcgg tgcaatggca ggggaaatga aacggccgga aaaagatttg cctaaagcaa
    13021 ttattcttgg tttgagtttt gtaacagtcg tttatctatt gatcaatttt gtttttttga
    13081 aaactttgcc gattgatcat cttgccggta atttgaatgc agcttctgaa gcatcagacg
    13141 tcatttttgg aggaatcggt ggaaagctgg tcactattgg gatcttgatc tcggtatacg
    13201 gtgcattgaa cggttataca ctaactggga tccgcgtgcc ttatgcgatg gctttagaag
    13261 acgagctacc tttcagtaaa cagctgacaa agctttcaaa gaagtttacg gttccttatg
    13321 ttcctgcggt tttccagtta gcagtagctt gtattatgat gagccttggt tctttcgact
    13381 ttttaacaga catgttgatt tttgtgatgt ggctattcag tttactgatt tttatcggtg
    13441 tctttatttt aagaaaaaaa gcgccggaat tgccacgtcc atataaagta ccactttatc
    13501 caatcgttcc gattattgcg attctcggtg ctattttcat tttaggtatg acgatgatga
    13561 cgcaaacgaa attagcattg atcggtatag gtgttacctt gataggtatc ccagtctatt
    13621 atcaaaaaaa gaaaaaacgc tcagaagagt aaaatatttc aacaacagac agaaaaagta
    13681 cgaactaggt cctctagatc ttcgtgcttt ttctatttca ataaaacaag ttcttctatt
    13741 ttgatttttg ctacttctgc aagtagtgct tatgtgctaa agtaagaatg aaaaggagag
    13801 ataaacatga tatttgattc tcatacccat ttaaatgctg aacagttcaa tgaagatatt
    13861 ccagaaacga ttcagcgagc acaagaaatg ggggtaacga aaatggcggt cgtgggcttc
    13921 gatacgccaa cgattgaaaa gtctttgcaa ttaagccatg actatccaaa tatatacagt
    13981 attatcggct ggcatccgac agaggctggc agttatacga aagaaatcga aaataaatta
    14041 caagaacaat tgactttacc aaaggttgta gcattagggg aaattggctt ggattatcat
    14101 tggatggaag acccgaaaga agtccaagat aaagtatttc gcagacagat tgctatcgca
    14161 aaagaaatga acctaccaat cagtattcat actagagaag cacttgaaga cacgtatcaa
    14221 atcctaaaag aagaagatgt tcgagatatc ggtggaatca tgcatagttt cagtggtgat
    14281 tatgaatggg caaaacgatt tcttgactta gggatgcata tttcatttag cggtgtcgtc
    14341 actttcaaaa aagcattaga cgtccaagag gctgcagcaa atgtaccaat cgaacgatta
    14401 cttgtggaaa cagatgcccc ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa
    14461 ccaggctata ctcgttatac agtagaaaag attgctgaac ttcggaatct tccgacagaa
    14521 gaagtggctg cccaaacatg gacaaatgcg caccgattat ttggtctaag cgaacatgga
    14581 taagatgaag atccaagaga ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg
    14641 ggaagtagtt gatgcagata cgatagaaac aatagggtct gcaatcaatg aagagatcct
    14701 gatgcaaatt gaacatgccc aagagactcg tggagtgatc gtttttaccg atcctgattt
    14761 ttcaggagaa aaaataagaa aaatcattat ggaagctgtt cctcaggcaa aacatgcttt
    14821 tttgccgcgc acacaagcta aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag
    14881 tgatgctgct attttagaag cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc
    14941 tgtttctgaa atcactagac aagacctttt agactatggt ttaatagctg gggcacaagc
    15001 aaaagaaaaa cgagaaaaac taggtgacga gttgcgcatc ggttacacga acggcaaaca
    15061 attggaaaaa cgtttgaaga tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa
    15121 aatagtggag gaacaacatg agtgaatatc gtgaaattgc gacaccctct agaacaaaag
    15181 agattttatt gaaacatggt ttttcattta aaaaaagttt aggacaaaat tttctgactg
    15241 aaccaaatat tttaaggaaa atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga
    15301 tcgaagttgg accaggaatc ggtgcattga cggaacaatt agccaagcat gcaaaacaag
    15361 tcgtagcatt tgagatcgat gatcgtttga tccctgtatt ggctgatacg atgcaaccct
    15421 atgacaatgt gactattgta catcaagata ttttaaagac cgatttgtca acagctgtta
    15481 gagaaacatt tcatgaggag cttccgttaa aagtcgtagc gaatttacct tactatatta
    15541 cgacaccgat catgatgcat tttcttgaat cagatttggt tgtggatgaa ctagtcgtca
    15601 tgatgcaaaa agaagtagct gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt
    15661 cattatcgat tgctgttcaa tattatatgg aagccagttt agcattcatc gtaccaaaaa
    15721 cagtatttgt ccctcagcca aatgtagatt cagcgatttt gaaactgaca agaagagata
    15781 taccggcagt cgaagtgaca gacgaaaaag catttttccg attgacaaaa gcggcatttc
    15841 agcaaagaag aaaaacgctt tggaataact tgcagcattc gtatggcaaa gatgaccaga
    15901 caaaagcatg gttagcaaaa agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg
    15961 agactttgtc attacaagaa ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt
    16021 aaaaaaagag gttgtgacag aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    16081 gccaaacaaa aatagttcct catgtttttc gtttgtttgg gcttattacc gcagatgcaa
    16141 gcttttgaac accgtttaag aaagaggttg tgggcctgcc gtttaactct aagtcacaag
    16201 gaacaatttc aaaaaacagc ttctcatgtt tttgagttag ttggtcttat tacttgatgc
    16261 aagcttttga ccactattat gccgaacaag gagatgtgac acaaattttg tcacatctcc
    16321 tatttttgtt tcttgatcaa aaaattctta tacttgatac tcacactcat tttgtatgga
    16381 tttagaaaag aatatgtgtc ttgacagtta ttagtaagtg agttatacta agtcgacatt
    16441 tatgtgtcaa gacatattag gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa
    16501 cgatttctgc tttattgatt gggatggggg tcattatccc aatggtcatg cctaaaatca
    16561 tgattccacc agcttccttt actttggcgt ctcatgtgcc attatttatt gcgatgtttt
    16621 ttacaccagg agttgcagtg gctgttgcgc taggtacgac atttggtttc tttttaacaa
    16681 cgccggttat catcgcattg cgagcattgt cacatcttgt gtttgcgttg attggggctt
    16741 ggtacttgca aaagcatccg agtatcgttt taaaaaatgg acagtttact tttgctaatt
    16801 ggcgtttcca agggttcaat tttgtgatcg gtgtgattca ttctttagca gaaatgctag
    16861 tagtcagcat cttttacttt attggtaata tgcctgaaac atattattct caaggatatt
    16921 tttatacagt attcatcttg atggggattg gtgggcttat ccacagttta gttgattata
    16981 atattgctta ttttatagca ggaacactca gcaaatattt tgatatcccc gtatttacgg
    17041 cagcaaaaaa agaaaatcgg aaagagtcga ttcaagggta cgcgaagaaa acgacatatt
    17101 agacatgatc tctcaaaaaa aaaactctca tctagtaatg gataataaat cctctaagat
    17161 gagagttttt tgtcctaaaa agagctgcac caacatcttc tggcacagct ctaacagggg
    17221 gcaatttatg gaagtttgat gacttgttct gtttctttat caaagaagtg gcctttgctg
    17281 atgttgaagg caaggtcgac tatttcgcct ggtttatgga aatctcttgc atctactttt
    17341 gagatgaact cagtagaacc agtttttgtg taaagcatcg tttcagctcc aagtaattcg
    17401 gaaacgacaa cttctgattt cactacggct tcaggtgaag cttcaagggc tacttgttca
    17461 ctgtggatgt cttctggacg aataccgaag ataacttctt ttccttcata acctttttca
    17521 actaatactt tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc atctgtgata
    17581 acaccatttt tcaatgtcac attaaagaag ttcatggcag gagatccaat aaagccggca
    17641 acgaacatat tgacgggtgt atcataaact tctttcggtg taccgatttg ttggataaat
    17701 ccgtctttca tgataacgat tcggtcagcc atagtcattg cttctgtctg atcgtgagta
    17761 acatagatgg tcgttgtttc aagacgctgg tgaagtttgg caatctcagc acgcatggct
    17821 acacgtaatt tagcatctaa atttgataat ggctcatcca ttaagaagac ttttgcatcc
    17881 cgaacgattg cccgtcccaa tgccacacgt tggcgttgtc caccagataa agcagctggt
    17941 tttcgtttca agtattctgt cagacctaag atctctgctg cattgtccac acgttttttg
    18001 atgtctgctt tatcatattt acgtaatttc aagccaaaag ccatattatc aaaaacagtc
    18061 atatgaggat acaatgcgta gttttggaaa accatggcaa tatcacggtc tttaggtgcc
    18121 acgtcattca tgacttttcc gccgatattt agttctcctt cagagatatc ttccagtccc
    18181 gcgatcattc gcaaagtagt tgactttcca caaccggaag gaccaacaaa aacgataaat
    18241 tcacggtctt tgattgatag attaaaatca gttacagaat aattttctgc attgtcatat
    18301 tttttatata cgtttttcag agccatttct accataatac gttccacact ttctttttta
    18361 gttttattaa ctatttaaag tataaatgaa agcgttaact aacttcaatg tcatgttgaa
    18421 caagaatttt ttcttttttt tgtcaatgtg ctagttttaa gctttaataa atatccataa
    18481 gaaataaaaa ctgatacact ggagtaaata agtatgttaa caatatcaaa aggagtgtct
    18541 cttatgaaaa tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt gaaattagca
    18601 acagcgtata aaacaatatt ggaaaaacat gaactttttg ctaccgggac gactgggcag
    18661 aggatcacgg aagcaaccgg cttgccgatc catcggttta aatccggacc gttaggcggg
    18721 gatcagcaaa ttggagccat gatttcggag gatcagttag atttggtgat ttttcttcga
    18781 gacccgctgg ctgcacagcc ccatgaacca gatgtaacag cacttatccg tttatctgat
    18841 gtctatgaga ttccgttagc tacgaatatc ggaacagctg aggtgctact aagaggactg
    18901 gaagccggct tcctagattt tcgaagaatc gtccatgaaa tagacaagca tccactttca
    18961 ttttgatgga taagcgtatg gaaaaataac gtttaatgtt cgcctatatg tagctataaa
    19021 agctaaacaa actgataata tgcgatataa tacgaacgta ttggataata taaacaataa
    19081 tattagtttt tcgtatttgc agtaaatgtg aaaatatgct atagtttctg tgggaaaaga
    19141 tcccaaaaat aatacagagt agaaaataaa gcgttaagtg ctaagaggat gggatgttgc
    19201 ctcttggacg aaggacgtaa tgttcgcggt tttattttcg cattcgctgc atgatacgat
    19261 gtagcagctc ttaaaggaga tgctaaaaat gaagaaacac cggttagacg cacgtatgat
    19321 tgctattatg ggcttattga ttgcattgat ggtcactttg tcccgactgg tagcaatcga
    19381 gaccccattt attaaaatca gtgtcacatt tattccacaa gtgatcatgg gcattctttt
    19441 tggtcctttt tggtcaggga taggcgcagt tttagctgat ttagtaggaa tggccctctt
    19501 ctcgaaatct gcattcttta ttggatttac attgaatgca tttattgaag gagcaatcta
    19561 cggattcttt ttctatcgaa aagagatcac ttggaaaaat gccattctgg ctacgcttag
    19621 tgtcacgctg atcatcaact tatttttgac accattatgg cttgcgttga tgtaccatgt
    19681 gccattgttc agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc
    19741 gattcaatca attgccattt attacgtagg acgtagtatt ccttacaaaa aaattttaag
    19801 aagtttagcg atccacgcta aataaaaaac aagcagtctt cccatcccag aagactgctt
    19861 gtttttattg tgctgtgaat tgtgcttgat agagccgtcg gtaataacca tctttttgaa
    19921 gcagctcatc atgggtgccc atttcgacaa tagctccctg atccatcact aatatctggt
    19981 ccgcattttt gattgtggac aggcgatggg caatcacaaa actggttttt ccttccatca
    20041 ttgctaagaa ggcttgttgg atttttttct ctgtcaatgt atcgactgag ctggtagctt
    20101 catctaagat caacataggg ggattactga tcatcgttcg agcaatcgtc aacagttggc
    20161 gttgtccatc tgatatcttt aacccttgac cgccaatctt agtatctaat ttttccggca
    20221 aacgttctac aaattcatac atataagagg cttttagggc atcattgatt tctgcgtcac
    20281 ttgcttccgg attcccaaaa cgaagatttt ctcgcaatgt actgtcaaat agccaagtat
    20341 cttgtaacac cattccaaaa cttttacgta atgtatcacg ttggatagag cggatgtcat
    20401 ggtcatcgac tgtaattgtt ccttttgttg tgtcataaaa acgcatcagc aaattaacaa
    20461 gtgtggattt tccggcccct gtttttccta ctatagcaat ggtttgacct ggataagcag
    20521 taaaattgaa atcttcgatc aaacgctgct tggggtcata agaaaaagaa acatgatcaa
    20581 aagagacttt tcctttgata tttttgggtt ccaaggcata ggcatctggc aaatcaggct
    20641 tttcttctgg ctgatcgatc aaatcaaatg ctcgttctag accagaaaaa gcggtttgca
    20701 gttgtgtcgt gattccagaa agttcgataa atggtttaga aaactgactt gagtagatcg
    20761 taaagctgga aatcacacca acagtgattg acgaaccact cttcaatgcc aataggccgc
    20821 caactaaacc aatcgataaa taagcaaggt gatctacaaa acgagaagac gggtttgtca
    20881 aggaagaaga aaattgtgcc cgttgtcctt tttcatatag atcagcattt agcagttcaa
    20941 attgctgttg actagcttct tcttgctgaa aagctttcac gattttctgg ttaccgattc
    21001 gttcggtcac aaagcccgag atatccccaa taatcctttg ctgtctatcg aaatttgttt
    21061 gtgaagcacg agcaacgatc caactcacaa ggaaaataat tggtgtagaa caaagaacga
    21121 ccaatgtcag aagtggactt agacgcatca taaaaataaa cgaaagaatg atgattgcca
    21181 ctccagaaaa taattgattg aaggccgcag aaattgcaat ggaaatattg tccatatcat
    21241 ttgtaaatcg actgacgata tttccgtggg agttttgatc ataataacga agtggcaatc
    21301 gattcagatg agcaaatgca tcttttctta attttgcaac ggataagtaa gctaaccgat
    21361 tgcttaacac ttggatgagc cattgactga ctacgttgat taaaacgata ccaccgaata
    21421 gaataagtaa atggaacagt cgattgaagc ggacctgtcc ttctccaatc atttgatcga
    21481 tactttgacc aatttgtaac gtcatcacaa ctgttgcaat cccattgatg attcctagta
    21541 tcacagctag ccaaatgtct tttgcatagg cagtaaggta aggaatgaat cggcggagcg
    21601 tctgcaagga taattttttt actttcataa ctattcttcc tcctgtgatg cgacgatttc
    21661 ctgatattct gctgattgag caagtaattc ttcatgggta ccctgagcag caatcttacc
    21721 ttgatccatc actaagatat gatgtgcttc ttgaatagag cggacacgtt gggagataat
    21781 aatcactgta ctgccaagat aagtctgtag cgccttacgc aaatccaagt ctgtttgata
    21841 atccaacgca cttaaagagt catctaaaat cagtaatgct ggcttggcaa tcaaagctct
    21901 tgcaatcgtc agtcgctgtt tttgtccgcc agagaaattt ttcccatttt ccatgacttt
    21961 tgtatctaaa ccatttggaa gctgacggac aaattcttct gcctgagcga ttttcagagc
    22021 ttcccaacat tctttttctg tggcgtcttt cttaccccat tgaaggtttt cacgaatagt
    22081 cccggtaaat agtacagaag tttggggaac aagagcaatt tgctgacgga gatgatccag
    22141 ctgccaagaa cgaacatcca ttccgtccat tataactgta ccggaactga catcatagaa
    22201 acgcggaatc aattgtgtca aagtcgtttt cccgcttccg gttggaccaa tgattccaag
    22261 gactgtattt cgcttcagct gaaatgtaat attttggaga gaaagaccag aatcaggagt
    22321 ataacggaag tcaacatggt caaaagcgat aattgcttca gaagaaaaat cgggagtagt
    22381 actgacctca gcttcaagaa tcgaattttc tgtgtctagc acctctttca cccgatttcc
    22441 tgaagcttcg gcacgtgtga agatcacgac gaggtttgaa acaacgatca gtgccaaaag
    22501 catttgattc atataattga tcaaagcaag aacttgtcct tgttgcaagc taccaatatt
    22561 tactttgatc ccgccaactg tcagcaaaca gatgatcccg acattcatga tcaaggttgt
    22621 agcaggtgat aatagggcag agatattgga aacacgtaaa taattgttcg ctagatcatt
    22681 tgtgctttta tcgaacttct ctgtttcttt ttttgtctgc gcaaatgcgc ggatgacacg
    22741 aacaccgcta agattctgac taacgtgttc attgagctga tctagttttt cttgcacttt
    22801 ttggtagaga ggaacggttt ttttgatgat aaaaaataaa atgattgaga aaataggcaa
    22861 taatgctaag aaaaacagac ctacttccca gtcgatcaca aaagccatga ccactgaccc
    22921 gatacttaaa aacggtgctc tgatcaataa acgaatgacc attgctaaag ctagctgaag
    22981 ctgattgatg tcgtttgtca ttcgggtgat caaggtatct gttccaaaat gattcaactc
    23041 gttgtgagag aaagtattga tttttttgat tagctgatta cgtaactctg taccaaatcc
    23101 ttgtgaagct actgaagcat agtattggca aatgatagca caaatcagac caatcacaga
    23161 catagcgacc atccataagc ccatttcaat gatttttcct cggtcattca tttttagtcc
    23221 attatcgatc aaggaagcca tcattaaagg caggacaagt tcgaagacag cttcaagaaa
    23281 tttgaagaac gggcccaaaa tgatttgctt acgatagtct tttgcataac gtaataactg
    23341 aatcatatga ttcctcctat tatttatttg tccacaattg aaaaaacaag tactaccaca
    23401 ttgtagctta ggaaatttag aatgaaaaga gaacaagcct tttcataaat gaaagtttta
    23461 ttatgaagaa gctttagaaa ttgaatagac tatttttttt aaaaaaaaga aaatgagaa
//
LOCUS       DAZKOM010000038        22986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000038 DAZKOM010000000
VERSION     DAZKOM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.038"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..727
                     /locus_tag="ACG8NF_001415"
     CDS             476..727
                     /locus_tag="ACG8NF_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471903.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="ACG8NF_001416"
     CDS             761..1039
                     /locus_tag="ACG8NF_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471904.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1636..1893
                     /locus_tag="ACG8NF_001417"
     CDS             1636..1893
                     /locus_tag="ACG8NF_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471905.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2006..2617
                     /locus_tag="ACG8NF_001418"
     CDS             2006..2617
                     /locus_tag="ACG8NF_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471906.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2796..3353
                     /locus_tag="ACG8NF_001419"
     CDS             2796..3353
                     /locus_tag="ACG8NF_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471907.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4052..4573)
                     /locus_tag="ACG8NF_001420"
     CDS             complement(4052..4573)
                     /locus_tag="ACG8NF_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0471908.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4772..8464
                     /gene="nifJ"
                     /locus_tag="ACG8NF_001421"
     CDS             4772..8464
                     /gene="nifJ"
                     /locus_tag="ACG8NF_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0471909.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8653..9540
                     /gene="cdaA"
                     /locus_tag="ACG8NF_001422"
     CDS             8653..9540
                     /gene="cdaA"
                     /locus_tag="ACG8NF_001422"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0471910.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9537..10706
                     /locus_tag="ACG8NF_001423"
     CDS             9537..10706
                     /locus_tag="ACG8NF_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0471911.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10730..12085
                     /gene="glmM"
                     /locus_tag="ACG8NF_001424"
     CDS             10730..12085
                     /gene="glmM"
                     /locus_tag="ACG8NF_001424"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0471912.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="ACG8NF_001425"
     CDS             complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="ACG8NF_001425"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0471913.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14371..14661
                     /locus_tag="ACG8NF_001426"
     CDS             14371..14661
                     /locus_tag="ACG8NF_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471914.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14674..15699
                     /gene="sppA"
                     /locus_tag="ACG8NF_001427"
     CDS             14674..15699
                     /gene="sppA"
                     /locus_tag="ACG8NF_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0471915.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15702..16361
                     /locus_tag="ACG8NF_001428"
     CDS             15702..16361
                     /locus_tag="ACG8NF_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0471916.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16518..17450
                     /locus_tag="ACG8NF_001429"
     CDS             16518..17450
                     /locus_tag="ACG8NF_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471917.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17604..18893
                     /gene="tig"
                     /locus_tag="ACG8NF_001430"
     CDS             17604..18893
                     /gene="tig"
                     /locus_tag="ACG8NF_001430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0471918.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19140..20390
                     /gene="clpX"
                     /locus_tag="ACG8NF_001431"
     CDS             19140..20390
                     /gene="clpX"
                     /locus_tag="ACG8NF_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0471919.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20473..21060
                     /gene="yihA"
                     /locus_tag="ACG8NF_001432"
     CDS             20473..21060
                     /gene="yihA"
                     /locus_tag="ACG8NF_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0471920.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(21134..21289)
                     /locus_tag="ACG8NF_001433"
     CDS             complement(21134..21289)
                     /locus_tag="ACG8NF_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0471921.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            21425..22753
                     /locus_tag="ACG8NF_001434"
     CDS             21425..22753
                     /locus_tag="ACG8NF_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471922.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            22764..22973
                     /locus_tag="ACG8NF_001435"
     CDS             22764..22973
                     /locus_tag="ACG8NF_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471923.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         7644 a         3912 c         4910 g         6520 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agtttattga taataatgta tgagatacag tataatgatt taaaaggtaa cgtgttgtga
     1501 ccgaattaaa tgaattaatg cctatttttt atattcctta gtggacaaaa atggagacag
     1561 tgaagttagg agcattgtat cctttgttgc gtagttcaaa aaagtaagcg atttctataa
     1621 tggtcagggt aaacggtgag ggctgtccac gtaaatccta ctattgaaat cacaatgatc
     1681 ggacattcaa aaggtggtgc tgaagcagca aattcagtag ctataaatac gaatgcgatt
     1741 atatttaatc cagcgacagt aaatttatct gcttatgaat tagactcaaa cacatataca
     1801 gcaaacatgg tagcttatgt tgtcgaggga gatgcattaa atgttgtttt tggaggagta
     1861 tacacctatt ggagaattaa tcatgttaga tagacaatat gatgcacctt agtggagaat
     1921 gatatctcgc ataggtaact cagtaaaaaa tcaaggtatg gaagcaatta tctctgcatt
     1981 ggaagatcga tatagatagg cggatatgaa tagtaatagg gggaataaaa tggaatacaa
     2041 tggatcaatg agaattgtac aaattatatt ttggattaca tcgggaattg tgatcatagg
     2101 aggagttttt ttgatgttgc caagcttaat atttcctttc tttgcattaa tctcacctaa
     2161 aatacccgaa ccggaaatca cctatggaga atttccattt aggattgaat acgaactaga
     2221 tggacagatt aacataattg aagatactgt catagctgag tttaacgggt gtgagttcag
     2281 cgcaggttct atgaaaagag aacgaagatg gagaagtaga ttagctagtg gtagagaaga
     2341 tctacctttt ggggacgatc taggaatcta ttttagtcga ggaagtgcgc aatattatat
     2401 gggggaaaat gtacaatcaa tgtcattaaa accacatata gcccttagaa atctcgagga
     2461 aggttttaaa agagaggtag actttatatt aggagaaagt ggatacataa gaactgtact
     2521 taatttcgga gaagcgcaag aagttttgac acaatacgga attaccttaa tcaactggga
     2581 aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga taaatagaaa
     2641 caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac gagtagtagg
     2701 aattcatagc gaatattatt acgaccagta gatattttga tattaaagga gtattacgaa
     2761 ctaatatgtg agaatgtaga ggtgatgtgc gaataatgag aaatttatca aatagaaaca
     2821 aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat gcggttttaa
     2881 gtagaataat attgggtcct aagccaccaa gaccagaaat tacccgaggg gagtttgatt
     2941 ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca attgttgcat
     3001 tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg tggcgtctac
     3061 atttagcaag tgatagacga tcacgaaata tcttattaga tgaattagag gatggtcgaa
     3121 gaatatatta tgtcccagaa agtgctaact attctatggg agatgtacag aaaaaaagag
     3181 agcctaatcc taattggtat ccatttaatg gcgtaacaat agaatatcca cgcaataaaa
     3241 cacctgaaat aggtgcaaaa tttataagtg gtttagaaga tctgtacgat aggtttggaa
     3301 tccgtcttat tagttgggaa cacgatccac caatagaaaa tcgctttgaa taatcgaaat
     3361 ctactaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
     3421 aaaccattga aatactaggc tgtgatcagc aaaaatgaat ctgaataaaa aagagagcaa
     3481 ctattagctg agaggagtta atagttgtta gctttatacg aatcaaagtg gcaaatacag
     3541 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     3601 gaaacaactt gtttaaaata aacgacctag ctcaacaaat gaccatctaa tcaactaaca
     3661 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     3721 ttgggatact agtgagaatc accgtacata aatattactc gttaagggaa attgagaaat
     3781 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     3841 tggtggagat aatttgctta gcttgtcata agaaatgaga taatctggca gatgcaagct
     3901 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     3961 aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4021 tctttacttt tgtcccacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     4081 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtt ggaacaaatc
     4141 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     4201 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     4261 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttgg ccatcgctga
     4321 ttgtgagata ccggtgcatc acgttccctc gctgtaagct ttctttttct ttatcgaaca
     4381 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     4441 cttcaaacca ttgtcggcaa tacggacaag ctaagttgat gaattcgacc atttttttag
     4501 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     4561 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     4621 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gatacatgta
     4681 aaatattttt ataaataaaa gaaaaaaatg gcatgtttta cgttataatg gaacagataa
     4741 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
     4801 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
     4861 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt
     4921 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
     4981 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct
     5041 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt
     5101 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
     5161 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
     5221 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt
     5281 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
     5341 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
     5401 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
     5461 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
     5521 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
     5581 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
     5641 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
     5701 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
     5761 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
     5821 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
     5881 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
     5941 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
     6001 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
     6061 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
     6121 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
     6181 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
     6241 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
     6301 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
     6361 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
     6421 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
     6481 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
     6541 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
     6601 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
     6661 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
     6721 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
     6781 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
     6841 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
     6901 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
     6961 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
     7021 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
     7081 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
     7141 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
     7201 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
     7261 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
     7321 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
     7381 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
     7441 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
     7501 ggaagactgg ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact
     7561 gcaagctgcg atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag
     7621 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
     7681 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt
     7741 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
     7801 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
     7861 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
     7921 aacgatcaag gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta
     7981 tacgccttgt atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa
     8041 agaagctgtt tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa
     8101 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
     8161 catgaggaaa caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc
     8221 tttatttgaa aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac
     8281 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
     8341 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag
     8401 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     8461 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     8521 aataaaacgc atacttgcaa ttttcttgaa aattatgcgt ttttctcaag aaaaatacga
     8581 gcatatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     8641 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     8701 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     8761 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     8821 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     8881 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     8941 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     9001 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     9061 tatatgtcca agcgaaaaat cggtgcgttg atcactatcg aacgtcatac tggattagat
     9121 gagtatattg agacagggat tgctttagat gcagatatca ctggggagct gctgatcaat
     9181 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     9241 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgatccctaa agaattcggg
     9301 actcgacacc gggcagctgt cggtataagc gaagtcagtg acgcgattac catcgttgtc
     9361 tctgaagaaa ccggggacgt cagcatcacg ttagataatg aactgatggc tggcttatcc
     9421 cagcaagaat atttggcaat cttgcgtaga gaactaattg ctgaagagaa aacaaaaaac
     9481 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     9541 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     9601 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     9661 ataatcaaac agttacgggg gtgccgatca agatttctta tgattcaaag cgctactata
     9721 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     9781 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     9841 gcgaaggaac acatgaagtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     9901 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     9961 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
    10021 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
    10081 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
    10141 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
    10201 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
    10261 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
    10321 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
    10381 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
    10441 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
    10501 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
    10561 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
    10621 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
    10681 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
    10741 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
    10801 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
    10861 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
    10921 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
    10981 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
    11041 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
    11101 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
    11161 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
    11221 attccttgta caaacgatta acggtgacct ttccggtttg accatttgtg tcgatgcagc
    11281 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
    11341 tacaatgggc acaaatccag acgggttaaa tatcaatgac ggcgtaggct ctacccatcc
    11401 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
    11461 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
    11521 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    11581 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    11641 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    11701 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    11761 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    11821 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgcg taagtgataa
    11881 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    11941 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    12001 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    12061 caaagaagaa atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc
    12121 agtgtcagat gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc
    12181 taaattacgt ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa
    12241 ttgagtcggt acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag
    12301 ctggtcactt tccttttcta atccctctga gacaataact agtgttttag cgccacggct
    12361 ttcgacttct ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc
    12421 gatcactggt gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc
    12481 aaaaccttct gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac
    12541 gttgtagtca gcaccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc
    12601 tgccaactct tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa
    12661 actcaattca tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat
    12721 agctttggct aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac
    12781 tgcgatttcg ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga
    12841 accagcaaca tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagcac
    12901 ttggcggctg tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga
    12961 aagaagaggt gtgttatagc caaattcact ggctaaatga acctcaacag gaatctgtgt
    13021 caatttttcc aaagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac
    13081 gatatagatt cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg
    13141 attgtcttct gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat
    13201 ttctttcagc atataaaaag gataggtacc tttctcgata tctgaagcat ctacttgtgc
    13261 ttcgaatgaa gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga
    13321 tgtgacggta accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat
    13381 agccattgca tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact
    13441 tttattttta gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta
    13501 agaacctttg atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc
    13561 gaaagtttca attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt
    13621 tgctaaataa gcaattttaa gttcttcaaa gttttcgatt acgccgttgt gaacgagcac
    13681 gaagcgtcca ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt
    13741 tgcccatcgt gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt
    13801 caaattttga attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa
    13861 gataccagct gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg
    13921 tgttacattt tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc
    13981 tataatgaat tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca
    14041 gacatttttt gtggattttc tgtagagtgc agtccctagg gccatccgcc gaatctttcg
    14101 ataaaccctc tcctcgtcat ctgtttacac agacctggcg ctatcctaaa aggtataact
    14161 atcctccttt ttgtttttta tctggaaaca tttaaaatca taaccttttg cgaatgttct
    14221 tgtcaacaaa aaaagaataa aaaataaatt ggtatatatc aaattctttg tcaaatgtcc
    14281 tttttatgtc aaaaaataat gggtttttat ctatcatctt tttcaggagt tcggtacaat
    14341 agaaagtaat cggatagagg aggagtaaaa atggattcaa aaattgagat catgacactg
    14401 ggaatgttaa aaaaacaatt gtccaaattc gaagcatcag ctggtgtatc agatgatacg
    14461 aaaatatttt tagatactgg ctgggatagc atccaagaaa tcgcacctga tgccttggaa
    14521 gttgctcaag ctcgtgagtt tacggtagaa gatgaattaa caaaagagtc gttttccggg
    14581 tatgcgcggg aagaaaaagc agagcggttc gacacttctg agcagtcaga aactgtgatc
    14641 gtcataaaaa acttgtatta gaaaggagaa agaatgaata aaagacgttg ggcagcagtt
    14701 ctgattgctg caggattatt tgtggtttcg ttagtttcag ctagtttgac taagcctcag
    14761 gaaaacgatc aaattgaggg attgaacagt tggttatatg gggatgaaga actagctcca
    14821 aatgtgttgg aagagggaga cagtacgcaa aaaatcgtaa aattatcagt agatggaacg
    14881 attgctgcgg gagggtcttc aggtttattt gcttctgaag gttacaatca taaaaatttt
    14941 atggaacaac tgaaggcgat tgaagaagat ccaacagtca aaggtatctt tttagaagta
    15001 aattctcctg gtggcggtgt ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt
    15061 cgtaaagaac atgatatccc aatgtatgtc agcatgaaaa acatggcagc tagcggtggc
    15121 tactatattt ctgctcaagc agataaaatc tttgcaacag aagaaacagt gaccggctca
    15181 attggtgtga tcatgtcagg attgaattat tcaggacttc tagaaaaact gggtgtggaa
    15241 gatacaacag ttaaaagcgg agcgttgaaa gatatgggtt caggaacgcg accagagaca
    15301 aaagaagaag aagcggtgct tcaagcatat atcgacagcg catatagtcg ttttgtagca
    15361 atcgtcagcg agggacggaa taaatctgaa gaagaagtca aaaaaattgc tgatggaagg
    15421 atttatgatg gtgtccaagc aaaagaagta ggtttagtcg atgaattggg atttcctgaa
    15481 gatgctttaa aagctatacg aaaggaacaa cagcttgaag atgcggaact agtagaatat
    15541 tcttccacgc catctgggtt tgctagtaca tggttaggaa gtaaactagc agaattccag
    15601 ggattgaagc cttctgagac tagtcagatc ttatccgttc ttgagaatct agggacagcg
    15661 gaatcaccaa aagcaatgta ttattacgga ggtgagtaag catgtcagag ctttcaaatg
    15721 aaaataagga acaagaaaaa gataagcaac agcagcctgc tgtaggacct gactccaact
    15781 tttcccaaaa ggtactgagc tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa
    15841 agaaacaacg taaatggaaa aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg
    15901 attttcctaa gtatttctat gcaggttttt ttatccgaat gtttgccttc ttggtcgatc
    15961 ttttgtgtat cggagctatt acgcgtttaa cacttggtat tgccaaaaat ctaggttggc
    16021 ttaatttttc agattcttat ctgagtctct acggattcac agccttactt atttatctag
    16081 cgtattttat tttattgacg aaattcaatc atggccaaac gattgggaaa atgatctttg
    16141 gtattcgagt catcagcttt gatgaagaag aacttagctg gtcaaccgtt ttagttagag
    16201 aaggtgcttg ccggttcatt ctaaaatttc ctttgctgat gttagggtat cttccagcag
    16261 tctttaatca gaaaaagcaa catctgggcg attttttcac taatacaagt gttgttacga
    16321 tcaatctgat caaagcgttc aaccaggaag tcaacagctg atgagcaata aaaagcagta
    16381 acagttgaaa gcaccttcta aaagtcagat ctaaaatcta gcttttagag ggtgcttcag
    16441 ttatttttaa ttgtcatgct ttttttgttt tgctactatt gaacatgagc tgaatatgag
    16501 caagaggagg acactgcgtg aagccgattg attttccaga aaaatatgag aatttaatgc
    16561 gagtggcaca acaagcacta gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac
    16621 gagcttacga gttgaaagaa agctttgagg ctaatagcct gcttgttttt tgtctttacg
    16681 agttggacga gaaaaaagaa gcattgaagc aagcactctt gcatgaaaaa cagtatcttc
    16741 aaaatgagga gtttgctgaa ttttattttg acttgttaat cagtacacaa gattttttat
    16801 atgctagaaa attgatagca agtacagatt tttatgaagc tttcgagcag cgggtcattg
    16861 aaaaaatcca atttgcagaa gaattatccg gacaaatgga gaggcagaag atcaaggcac
    16921 tccacaataa atcggaggaa cttccttcgt tggaacctgc acgtcagctt gcgtctattg
    16981 atgagatcga acagcttcct tatcatgagt ttatccaaac agcgagtaaa cttattgtat
    17041 cgccagaggt ccatatttta gcaagggcaa aattactgga gacacttagg caattgaacg
    17101 aatgccatca agttttctat ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag
    17161 atctgccaaa gccgcagcag caggcaagtt accagcaatt atgtgtattt tcagatcgtt
    17221 acgggaatga agatgcgctg cttagttccg tgttaaaaga agaattcacg ttgcaatctg
    17281 caattgttta tcctgtctat gatacataca tcgaagaccc gaagcgctgg tttcaactga
    17341 ctgtagaggc gtgtacagga aaaacgatgt atgaaggtac agaagatgaa aaacaagatt
    17401 ttttaaaaaa aagagaaaaa attttgcagc aaatgatctt cttccattag attttatcag
    17461 tgaaaaagat aaaaatgtta tgaaaaacaa ggattctatc cattcaatat gtttacaatt
    17521 tgaaagggtt agtgtactat ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa
    17581 tttctaaact cggagggaat taaatgactg caacatttga aaaaaaaggc acgaatgatg
    17641 gtgttttaaa atttacgatt gcgcaagacg aaatccaaaa aggattaaca actgcgttta
    17701 ataaagtgaa aaaaagcttg aacgtacctg gattccgtaa agggaaagtt tctcgtcaag
    17761 tattcaaccg tatgtacggt gaagaagctt tatatgaaga tgcattgaat gctgtattac
    17821 cagaagctta tgaagctgct gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa
    17881 tcgatgttga aagcatggaa aaaggtcaag actgggtaat cactgctgaa gtaacagtga
    17941 aaccagaagt aaaattagga caatataaag atctagttgt tgaaaaacaa gaccgtgaag
    18001 taacagatgc tgacgtggat gcacgtatca aacgtgaaca agaagcacaa gctgaattag
    18061 ttatcaaaga agatgctgca gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct
    18121 ttgtagatgg cgaagcgttt gatggcggaa aaggcgaaaa ttactcactt gaattaggtt
    18181 caggctcatt cattccaggt tttgaagatc aattagttgg tcataaatca ggtgaaagtc
    18241 tagatgtaaa tgtaactttc ccagaagatt atcaagcaga agatcttgct ggaaaagaag
    18301 cagtcttcaa agtgacaatc catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg
    18361 aattcgcaaa agatgttgat gacgaagtat caacattaga cgaattgaaa gaaaaatatc
    18421 gcaaagaatt agtagaaaca aaagaaaaag cagcggacga tgcaaaagat gaagcagcga
    18481 tccgtatggc tgttgaaaac gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg
    18541 aagtacatcg ttcaatggac gaattcttga acaacatgca acgtcaaggt atctctcctg
    18601 aaatgtacta tcaattaact ggttctacag aagaagactt gcataaacaa ttcgaaggtg
    18661 aagcagaaat gcgcacaaga acaaatcttg tgatcgaagc aattgcagct gctgaaaatc
    18721 ttcaagcaac agaagaagaa atcaatgcag aaatccaaga attatcagaa acatacaaca
    18781 tgccaatcga acaaatcaaa cgcgtgttga cagaagatat gctggaacat gatgtgacaa
    18841 tgaaaaaagc aatcgaattg atcacaggta cagcgacaga agcgaaagaa tagaaatcaa
    18901 gtaataaatc atttctgaag aagctacttc tagaaaattg tttattcgaa ataagggact
    18961 gtgacacgat tttgtcacag ttccttattt tttgttaaga gtaaaaaatg caaaggtata
    19021 ggtcagttga tcctttgtta aacacttttt ctttttacta aactatgtta tgatagtaaa
    19081 agactgtaaa gtctttgata gtttcttgca tactttttgc aagttaaggg gtgaagacca
    19141 tgtatgacaa tccaagtagc aatgagaccg tacgctgttc tttttgcggg aaaacccaag
    19201 aagaagtgaa aaagatcgta gctggtccag gggtctacat ctgtaatgaa tgtatcgacc
    19261 tatgtaaaga aatcattgat gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag
    19321 tccctaaacc actagaaatt cttgaagtat tgaataacta cgtgattgga caagatcgcg
    19381 caaaacgctc gttagctgtt gccgtataca atcactataa acgtgtcaat cagcaagcac
    19441 aagaaaacac agatgatgtt gaactgcaaa aaagcaatat ctgcttgatc ggacctacag
    19501 ggtctggtaa aacattttta gcacaaacat tagcaaaaac attgaatgta ccttttgcaa
    19561 tcgcagatgc gacaagtttg acagaagctg gttatgtggg ggaagatgta gaaaacattt
    19621 tgcttaaatt gcttcaatct gctgattaca atgtggaact tgcagaaaaa gggatcatct
    19681 atattgatga gatcgataaa attgcgcgta aaagcgaaaa tgtatctatt actcgtgacg
    19741 tttccggcga aggtgtccaa caagccttgc tgaaaattct agaaggaact gttgcaagtg
    19801 tgccacctca gggcggacgt aaacatccac atcaagaatt tattcagatt gatacgacga
    19861 acatattgtt catcgtaggt ggtgcatttg atggcatcga aacgattgtg aagaatcgta
    19921 tgggcgaaaa gacgattgga tttggtacac aaaaccaaaa actttctgaa gacgaaagtg
    19981 tcatgcagca tatcattcct gaagatttac taaaattcgg attgatccct gagtttatcg
    20041 gacgattacc tgttatggca gctttagaga aattgacaac agatgatctg gtacggatct
    20101 tgactgagcc gaaaaatgcg ctagtcaaac aatatcaaaa acttctttct ctagataata
    20161 ccgaacttga attcgaacct gaagcgttga gagccattgc gaaaaaagca atcgaacgca
    20221 ataccggtgc tcgaggactt cgttccatca ttgaagaaat catgatggat gtgatgtttg
    20281 atatcccttc aaatgaaaac atcgaaaaag tgattatcac aaaagaagca gctgaattgt
    20341 ctggtgagcc aacagtaatc tataaagatt cagagaaaaa agcgggttga tgaaaagagg
    20401 ctgccgtaaa agtcattttg gcttttgcga cagccgcttg ctttattcat caccttgtta
    20461 aggagagaga ccatgaaagt ccatcaagct gaaattatca taagtgcagt ggcaccaaaa
    20521 caatatccag atacacaatt gccagaaata gctctggcag gacgatcaaa tgtagggaaa
    20581 tcttctttta tcaacacatt gattgatcga aagaatttag cccgtacttc cggtaagcca
    20641 ggaaagaccc agacattgaa tttttatttg atcgagaacg cattacattt tgtcgatgtt
    20701 ccgggatatg ggtatgcaaa agtatccaaa actgagcgag caaaatgggg aaagatgatc
    20761 gagacatatt taacgactag agaccagcta cgtgcggtcg tttctttagt agatttgcgt
    20821 cataagcctt ctcaagaaga tatccagatg tatcaatttt tgaagtatta tgatatccct
    20881 gtaattgttg ttgccactaa ggcagataag atccctcgtg gtaaatggaa caagcatgag
    20941 agcgaaatca aaaaagcgtt ggattttgat cgcaatgaca cgtttattct cttttcttcc
    21001 gttacaaaac agggaaaaga agaagcgtgg gcagcaatcg aacaagcaat tgaatcataa
    21061 aaagaataaa aaagacttac cagtcaaact ctttcataaa agtttggctg gtaagtcttt
    21121 ttttgagctt aacttacttt tgtttcttgt cttttttctt ttcattttgt ggctgtggtt
    21181 ttagaaatcg atcgatctct tcttttggtg caactgtttt ttccggatcg acagcaaatg
    21241 attcgaaaag tttatctaaa ttatctgtac gttcaaattt caaccccatt gtaaacacct
    21301 ctttgtttct ttttacttag tctatcatgt aatcgtccaa attggtttct aagacataaa
    21361 gttttagttt ttctttatca ttggttataa ttaaattata aaaagatttg aaagaggtga
    21421 gaaaatgatt ggcgaagata aattattgat gactatccag ctcacagact taaatgaaga
    21481 agaaaaaaaa caggaaataa gcgattgggc tattttgaca cgtctgttga tccagccaac
    21541 atttatccgc tctttcacac aacaagcgga tccgtatgat cttcgaaagc ttcaagaaaa
    21601 aataatgcta gcatcggtaa gagacgaaag ttggttagtt gtaggaaatg acgagaacga
    21661 atgtagtttt cgcttggaag ataatcaatt attgataaaa aatgtattgt ctatctctgt
    21721 tttcaaagaa aagcaatttt tgattcgaga cttcattcag aaaaaaatga ttgctcatgg
    21781 ggtgttcgcc tatatgcgag cttatagcga gtttatctat cacaatacaa aacagatctc
    21841 tcaacggcta ttgtttgaga aaaaggatga aatcgaacat ttacctaaaa tgaaacaaca
    21901 aaacggggaa gtagtagtcg attgcaacca atttccagga tacgatcttt tttaccaagg
    21961 tttatgtttc acttcgtgtt gggaaatgta ttatagtcgc tattatcatc aaatcatccc
    22021 taaaccgatt tttctcgaca tccaacaagt agaaaaagtg aaggaattag aaaacgaagt
    22081 catatgtatc cagctttatc gagacccctt taactggcaa aagccaaaca atcaaatgtt
    22141 tcaatcctat tttagggacc agcttgggtt tgatcatctt gcttgggaca atggtgtcgg
    22201 tttattgaaa ccgccattcg ttgaatacat ttacactgac catacgatcc aatctgtcca
    22261 gtatcagaat gcacaaatgc aacctgttcc taaaaagaat gcttcatttt ttgtaacaaa
    22321 aagttatgat atccaacaag gagattataa agaaaggcgt gttcgcggaa cactaaatgc
    22381 tcaggcgtat tttccttggg ttgacgagaa tcgatcacgc atgatgtgct ataaagttat
    22441 cgatccgact gttgctttgg ataatggaat cgaagcttat tgttattata ttagggaata
    22501 tctagaagta gaggtaacag atgaaaaata tcaagagtat cttttatcct taagaatcta
    22561 tgttccttca gaatccctgt cagaattacc gttaaaggaa atcaaacata gactctctga
    22621 tgttaccttc aaacgaataa agaaaaagcg gcgaagtatc caattcgatg tcaagaaagg
    22681 tgagcagcat ctgcgtgtgc aatttttaga ttaccgagaa ttagaacaat tgattacttt
    22741 acaaaaaata tgaggtgaag aaaatggata aacaactcta ttatcgaata atggttccgg
    22801 catcattatt atccgtctgt ctcttttttg ttcctaattt tcctattcga tcagtcatcg
    22861 gttttttggt catgtttggc gggatgggac tttattatta tcttcttcat aaggaaagaa
    22921 aacctgcttc tgtttatatg aaaaaaacaa gaagcgaaaa taaaaaacga taaaacggct
    22981 tgatga
//
LOCUS       DAZKOM010000039        22399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000039 DAZKOM010000000
VERSION     DAZKOM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.039"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8NF_001436"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8NF_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0471924.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8NF_001437"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8NF_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0471925.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8NF_001438"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8NF_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0471926.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8NF_001439"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8NF_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471927.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8NF_001440"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8NF_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0471928.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8NF_001441"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8NF_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0471929.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8NF_001442"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8NF_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471930.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8NF_001443"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8NF_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0471931.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8NF_001444"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8NF_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0471932.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8NF_001445"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8NF_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0471933.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8NF_001446"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8NF_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0471934.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8NF_001447"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8NF_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0471935.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8NF_001448"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8NF_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0471936.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8NF_001449"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8NF_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0471937.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8NF_001450"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8NF_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0471938.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8NF_001451"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8NF_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0471939.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8NF_001452"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8NF_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471940.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8NF_001453"
     CDS             13967..14695
                     /locus_tag="ACG8NF_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0471941.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8NF_001454"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8NF_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0471942.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8NF_001455"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8NF_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471943.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8NF_001456"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8NF_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471944.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8NF_001457"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8NF_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0471945.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8NF_001458"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8NF_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471946.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG8NF_001459"
     CDS             16394..16606
                     /locus_tag="ACG8NF_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471947.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG8NF_001460"
     CDS             complement(16639..16833)
                     /locus_tag="ACG8NF_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471948.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG8NF_001461"
     CDS             complement(16846..17028)
                     /locus_tag="ACG8NF_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471949.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG8NF_001462"
     CDS             complement(17025..17165)
                     /locus_tag="ACG8NF_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471950.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG8NF_001463"
     CDS             complement(17655..18020)
                     /locus_tag="ACG8NF_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471951.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG8NF_001464"
     CDS             complement(18060..18362)
                     /locus_tag="ACG8NF_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0471952.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG8NF_001465"
     CDS             complement(18362..18661)
                     /locus_tag="ACG8NF_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471953.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG8NF_001466"
     CDS             complement(18654..18818)
                     /locus_tag="ACG8NF_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471954.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG8NF_001467"
     CDS             complement(18824..19093)
                     /locus_tag="ACG8NF_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471955.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG8NF_001468"
     CDS             complement(19105..19854)
                     /locus_tag="ACG8NF_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471956.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACG8NF_001469"
     CDS             complement(19851..20543)
                     /locus_tag="ACG8NF_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0471957.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACG8NF_001470"
     CDS             complement(20549..21220)
                     /locus_tag="ACG8NF_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0471958.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACG8NF_001471"
     CDS             complement(21213..21554)
                     /locus_tag="ACG8NF_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471959.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..>22399)
                     /locus_tag="ACG8NF_001472"
     CDS             complement(21732..>22399)
                     /locus_tag="ACG8NF_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFQ0471960.1"
                     /translation="AVTTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPD
                     SYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDM
                     MIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKK
                     VFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGD
                     DNE"
BASE COUNT         6184 a         4739 c         3485 g         7991 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagctt
//
LOCUS       DAZKOM010000040        22172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000040 DAZKOM010000000
VERSION     DAZKOM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.040"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..2077
                     /gene="tdc"
                     /locus_tag="ACG8NF_001473"
     CDS             242..2077
                     /gene="tdc"
                     /locus_tag="ACG8NF_001473"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0471961.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2382..3860
                     /locus_tag="ACG8NF_001474"
     CDS             2382..3860
                     /locus_tag="ACG8NF_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0471962.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3826..5157
                     /locus_tag="ACG8NF_001475"
     CDS             3826..5157
                     /locus_tag="ACG8NF_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0471963.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5271..7823
                     /locus_tag="ACG8NF_001476"
     CDS             5271..7823
                     /locus_tag="ACG8NF_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0471964.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7830..9269)
                     /locus_tag="ACG8NF_001477"
     CDS             complement(7830..9269)
                     /locus_tag="ACG8NF_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0471965.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9244..9426
                     /locus_tag="ACG8NF_001478"
     CDS             9244..9426
                     /locus_tag="ACG8NF_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471966.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9615..9977
                     /locus_tag="ACG8NF_001479"
     CDS             9615..9977
                     /locus_tag="ACG8NF_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0471967.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10164..11021)
                     /locus_tag="ACG8NF_001480"
     CDS             complement(10164..11021)
                     /locus_tag="ACG8NF_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0471968.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11141..12052
                     /locus_tag="ACG8NF_001481"
     CDS             11141..12052
                     /locus_tag="ACG8NF_001481"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0471969.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12059..13705
                     /gene="mdcA"
                     /locus_tag="ACG8NF_001482"
     CDS             12059..13705
                     /gene="mdcA"
                     /locus_tag="ACG8NF_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0471970.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13807..14106
                     /locus_tag="ACG8NF_001483"
     CDS             13807..14106
                     /locus_tag="ACG8NF_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0471971.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14096..15772
                     /gene="mdcD"
                     /locus_tag="ACG8NF_001484"
     CDS             14096..15772
                     /gene="mdcD"
                     /locus_tag="ACG8NF_001484"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0471972.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15776..16369
                     /locus_tag="ACG8NF_001485"
     CDS             15776..16369
                     /locus_tag="ACG8NF_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0471973.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16393..17469
                     /locus_tag="ACG8NF_001486"
     CDS             16393..17469
                     /locus_tag="ACG8NF_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0471974.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17494..18363
                     /gene="mdcB"
                     /locus_tag="ACG8NF_001487"
     CDS             17494..18363
                     /gene="mdcB"
                     /locus_tag="ACG8NF_001487"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0471975.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18474..19238
                     /locus_tag="ACG8NF_001488"
     CDS             18474..19238
                     /locus_tag="ACG8NF_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0471976.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19727..21820
                     /locus_tag="ACG8NF_001489"
     CDS             19727..21820
                     /locus_tag="ACG8NF_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0471977.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21845..22147
                     /locus_tag="ACG8NF_001490"
     CDS             21845..22147
                     /locus_tag="ACG8NF_001490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0471978.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3787 c         4724 g         6484 t
ORIGIN      
        1 tttgtttgtt cgcttttttt aggttatcta ttttagaaga ctgatttatt gaaaaattgt
       61 tcgctttttt gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa
      121 tctacgtgga tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg
      181 tgttacagat aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa
      241 aatgaaagat atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt
      301 ttataaaatg ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat
      361 tccttcagat atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac
      421 ccgcgaccat atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat
      481 cccatggcat tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc
      541 tttattagct tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc
      601 tatggcaact tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat
      661 gacagacgga tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg
      721 gtacgcccgt tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt
      781 gaaaaaaatg tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac
      841 cgaaagcttt acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa
      901 aaatatccaa agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa
      961 ggcgttagat atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga
     1021 ttatcgaatg gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat
     1081 cccaattcta ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt
     1141 agataaaatc gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca
     1201 tgttgatgcc gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt
     1261 tgtgccgtat gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt
     1321 aacgattgac aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac
     1381 gatagatcct cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca
     1441 taaaaatatg agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa
     1501 ggctccagat atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc
     1561 cgcagtctgg acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat
     1621 tggggcttca atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac
     1681 tgttaaagga aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa
     1741 ttgggtattc aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa
     1801 aatgttcgat cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc
     1861 tcatactaca tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat
     1921 gggtctaacg aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat
     1981 catgactcct tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa
     2041 agcaatggaa aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat
     2101 aacaagagac atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc
     2161 ctgtttttca tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga
     2221 tcgttcgttt catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat
     2281 actgggtttt gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa
     2341 gaaaacaaaa acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat
     2401 caaagcaaat cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac
     2461 gaaatattcc agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag
     2521 tattattgta cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc
     2581 aaaaaggtgg tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg
     2641 tctcatggct gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg
     2701 ctccgttgtt tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat
     2761 tagttgtcat tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg
     2821 caaaaatcgg cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc
     2881 tcttattagg gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca
     2941 gtttttcatg ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg
     3001 cgccgatcat gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg
     3061 aaaatccagt taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg
     3121 tcaatatttt gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga
     3181 ataatatcgc gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg
     3241 tgaatctgtt tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct
     3301 caggaccagc aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca
     3361 atttttggaa gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca
     3421 ttatctcaat ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga
     3481 tgctcgtaaa ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag
     3541 ttttgagatt gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag
     3601 agaaaaagag caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat
     3661 tttctgtcgg actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca
     3721 tcagtgtcat tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg
     3781 aacaggaagt cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca
     3841 atgaaacgga ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga
     3901 acaggattat ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc
     3961 gtttatttga tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt
     4021 ttagcaaata gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag
     4081 tgggcatttt ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa
     4141 ttaacagcaa taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc
     4201 ggaggtagcg cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt
     4261 gaattggaat cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg
     4321 ggtataggat tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt
     4381 ggacagatcg atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg
     4441 gttttattca gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat
     4501 ccgcaaaaga atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat
     4561 ataggaacga ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa
     4621 agtcctttag tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat
     4681 ctaatgattt tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg
     4741 attttatatc aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag
     4801 aaaggcgtac ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt
     4861 ctacattttt ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg
     4921 agttgttttc tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa
     4981 aagaaacgga acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca
     5041 attgctttca gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc
     5101 tggtttgtat tcttaaattt tgtatataaa agaataagca atagaaatat cagttaggcc
     5161 cagatacatt ttgcaaaacg aattatttca gacccccctg aaataatcga tacgcctgaa
     5221 aaaacttccc tgcgtataat aacagtatca attaaacagg aggggatagg atgaactggt
     5281 acagattgac ggtagaagat gtattgaaaa actatcaaac atcaacagaa ggcttgtcgc
     5341 aagaagaacg acagaaacgc ctgactgatc aaggatataa tgaaataaaa gcaaggcagc
     5401 agacaaaacg atggaaaaaa atagcaaagc attttactga tctgctgatg atcgtgttga
     5461 tcattgcctc ttttttgaaa tttgcttcta gtgaatatat tgaaggaagt attattttat
     5521 tcgtagtgat cgtcaatgga ctcgttagtt actggcaaga aagaaaagcg gaagaatcat
     5581 tgaatggttt gaagcagctg atgggacaag aagcgatagt actatcaaac gggatgcaag
     5641 aaaaaatccc tgcgaaagaa ctggtaaaag gagatgtcat tgtcttacgt gccggagatg
     5701 tcgttccagc agatatccga ttaatcgaag cacatgattt attagtggaa gaatcgattt
     5761 tgacgggaga atcagaagca tctgaaaaaa gtcatgccgt tttgacagaa gaagaatcga
     5821 tcggcgacca gaaaaatatg ggtttttcag gaacgcttgt acaatctggt tctgcagtcg
     5881 gtgtcgttgt agaaacagga aacacgacag aaatcggtaa aatcaatcaa gcattgcaat
     5941 cggtcgaaca acaaacgacg cctctgatta aaaaaatcaa ccgattaaac aaacaaattt
     6001 ttcaaggcat tctttgtttg agcctattct tagttatttt tacgacattt cgatatggta
     6061 tggattggca tatccttctt tcggctacga ttgctttggt ggtgtcaatg gtacccgaag
     6121 gattgccagc tgttttgacc atgattctat ccgttggagt acatgaaatg gcaaaagaaa
     6181 aggcgatcat caaaggactt ccttcggtag aaacattagg ttctatgaca gtgatctgtt
     6241 cagataagac aggaacattg acaaaaaatg aaatgacagt tgtggatgta gctgcaaaag
     6301 aagaagcctg cgtgttgtcc attatgaaaa actgtcagga attaaaaaca aaagaagaac
     6361 agaaaacaga aaatttaagt ggcaatccga cagaagttgc gctgattcga cacacagaaa
     6421 cagccaatct acctttgaaa aaaatagaag caaagattcc gttcagttct gaatacaaat
     6481 atatggcaac gatgcatgcg gaggaaaaag gagcggtgat ttacgtcaaa ggtgcaccag
     6541 aagtattatt tgctaaatca actctttctt ctgcagaaca agaagcatgg agtcagacag
     6601 ctgcagaatt tgcgagaaaa ggtcagcgtg ttttaggatt tgcttataaa aaagtagatt
     6661 cgaaacagga actcacgcat gagactttaa cccaattaac ttttgcaggg atcgccggat
     6721 tgatcgatcc gccaaaagaa agtgcagtca aagctgtaaa ggaatgccaa caagcaggga
     6781 tctcagtgaa aatgattaca ggggatcaca aagacacagc aaaagcgatt gcggaacaaa
     6841 ttggcttaaa acatactgcc aaagttttag aagggatcga tttggatctt atgtctgatg
     6901 aagagttgat tcagcaagtg ccaatagtcg atgtctttgc tcgaacgaca ccagaacata
     6961 aactgcgaat tgttaaagct ttgcagaaaa atggagaaat cgtcggaatg accggagacg
     7021 gcgtaaatga tgcacctgct ttgaaaagat ccgatgtagg aattgctatg ggaatcaaag
     7081 gtagtgaagt aagcaaacag gcagctgata tggttttagg agatgataat ttccatacga
     7141 ttgcaaaagc cgtaaaagag ggccggcgga ttttggataa tttgcaaaaa accatcaatt
     7201 tcttcttgcc aactgcatta gctcaaggat tgatcttgat ttgggcactt atgctgaatc
     7261 gcccgctacc tttgtcccca gtacagattt tatgggtcaa tatggtaacg acaatcactt
     7321 tatcttatgc actagggttc gagtcgccag atccagaaat catgaagcgc ccaccaagag
     7381 atccaaagca aggaatccta tctggttatc atttgtttcg gatcatctat gtctccctac
     7441 tgatcatggt tcctgcctat ttattggcta tgcagtttga agggcaaagc ttacagcaaa
     7501 ccattcttct gcaaaatatc gtcttagctc aagctgttta catgatcaat tgcagagagt
     7561 tgtccaagcc gtcattgaac caaggtcttt tgcaaaacaa agcgcttttt ctttcattag
     7621 gtatccttgc tttattgcaa gggatggtta tttttctacc agtcggccag cagctgatcg
     7681 gtacagtaag tttaacgatg acacaacagt tattgattgg agcaaatgtg attcttctat
     7741 tcttagttgt agaaatagaa aaagggatca tgaacaaact gtttcgcaaa aaagaaaaaa
     7801 cagctagaga atacagtcgc tagccgttac tataaaaaag ttttgaaagt gagttcagtc
     7861 aaacgattga gctcattttt cgttggaata gaagaaatgt atacaatagg gatatctaga
     7921 gaatctagag ggaaattgga aatcaagata tctgtttttt caaaagaaat agtggaaaga
     7981 tcggttagtt cgataggtaa gaagcagaca cgttttccta agtaatcttt taattcctgc
     8041 tgcaagaacg ccttccatga aggttctcct tggaaaataa aatagacatt gaacaaattc
     8101 ggtttgatag aaaaaatcgt ttgttggatc agtagagaca agagttggag aaagtaattt
     8161 ggttcattga tttctatttc tttttttaga tcgttcagta aattctcttt ctgttgatac
     8221 aagtgtgcat attgctgatc atatgtttct aaaaaacgat ggtattgacg gtccaatgct
     8281 tcaatcaata tcggcgattc atgatatttt aatatgagga gagccaagtt atcagtcaga
     8341 gatgtttttt ttagttgatc aagagacagc tctgaagcta gttcctctac aaacgtttcg
     8401 aggacctcta aaaatggaga atgatgcagg cagcgagcgg tttcttgtcc atagttattg
     8461 ttgtaattcc aactttccag aaagcagaaa gaagcaaaag aaagctcagt gtctttaaga
     8521 taaatacctt ccgttaaata caattttttc aatgcagagt catacaggga ggagagtgtg
     8581 ctgttggtat ggatagaaga atctaatcgg caattcgctt ttatacggat cgtattgata
     8641 aagaaccaac atgttccgaa aatctcttct gtttcaaccg ctagttcagt ttgactgagg
     8701 tttcttaaaa attgaaaata atgggattca tggatcgaat aatcctcgaa aaaatagttg
     8761 ttatgagtga aaggaatcag caatcgatga taaaaagtac gaatcgcacg ctcttttcca
     8821 agaatacaag ctgtatattt cgtcaaatcg atctgcaagt cgtaacttgc taaacgctga
     8881 ttgattttag tcacatgtct tttcaatgtc ccataagaaa gcccatggtc ggctagaaaa
     8941 tcagggacag caactttttt tgtaaaaagc agatttttca gcagttcgac actgactgat
     9001 tgtttgaaaa aaatctccca aaggtcattt gttaataaat tttgctggtt ttgcaaactg
     9061 attcctgctt gatcgctgat gatgtcccag ccttcgggca gctgagagcg aatagattga
     9121 aggtcagaaa aaatcgttcg ttcggttgtc cctatgattt cagctaattc atttacggtt
     9181 atctttgtat gattcagcaa ttgttctact aaagaaacat gacgtcgaat atctttttcg
     9241 gtaatgattt ttttcattag tgaatacatc aatcagactc ccttctattc taagaatacg
     9301 gagaaaataa acggtggtca agtttcaact gatttttcaa tcattgttta tttttctgga
     9361 gcatttactg atgataagaa tttcatcatg aacttgaaaa atgataccac taaaatcaaa
     9421 aaataggtgg gcaaacaata ataaacattt taatagtaga tggtagcaag accaacacag
     9481 gcggattgaa catttaagag ttgataaaaa agaaaaatat cgaagtattt gattagcaac
     9541 tacttgtatt catttttcta ttcttacgtg gtatctttga ataaagcgaa tcaataagaa
     9601 agagatggaa gcagatgcta acgaaaaaag aactaatcga tcacatcaat gaaacataca
     9661 atttgtctcc agaatatatt tttaaaaaat ttcctgatta ttgcgtattc agacatcaaa
     9721 aaagcaaaaa atggtttggg gtcctgatgt ctgttccaga agaaaaaatc tatgatgatg
     9781 caagtgacca taggatcatc aatattcttg atttgaaggt cgatcctgaa cttggcggaa
     9841 ttttgcgagg aaaagagggg tattttccag cctatcacat gaataaagaa cattggatct
     9901 ctattgatct acgtaaaata gaaaagctag atgaactagc agatttgatt gatggaagtt
     9961 ttaaatcaac gcaatgaatg tctaggacaa tagagaagag gttgtgacag aaatgtttag
    10021 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttggg aaatttcggc
    10081 ttattttcta agaaattact tctggaacac cgtttgttcg aaaaaagagc tgcgacaaga
    10141 attttgtccc agctcttttt tttttatttt cctaaataaa attcccgcat cgtttttgca
    10201 atcattttga tcaaaggatg atcttctgtg aagctagaag ataaataaat tgttcgtgtc
    10261 aagggcaaat ctttgatttc ccgcaagacg aaaggatcat ttttatcgta agaccaagaa
    10321 acttttggga aaaagctaac agctaatcct tgcttgacaa tagaacgtaa tgtcgcagga
    10381 tcatctgtcg tcgaactgat cgtcaattcg atacttttcg cttcaagtgc tgcatccagt
    10441 gtatgacgga aggcgttgct ctttgacagc atcaagatgg gggtgcgttg gatatcttct
    10501 aattgcagat gttctttttt agcaagagga tgtttcttag gaatggcgac ataaacaggt
    10561 tctttcatga taggaatatt ggtcatgcca ggcttcatct ccgaagtgat gtacagtaca
    10621 ggatcggtaa tatcatctgg taaatgatgt tgcatgatcg ttaattggat atcaggatgc
    10681 ttttccataa aaattcggac caagttcgga atagaaacag aggctacctc taaatacaat
    10741 atgacggaag taacgatctt ctcattgacc gaagacaata atttgattcc ttcttctaat
    10801 gtgtctaatg tcttgactgc atatggataa aattcttctg cataaacggt cgtctcaatg
    10861 tttctgccgc tatggtaaaa caaagggaca cctaattcgt tttccaaatg tttgatcgca
    10921 cggctcaagg caggttgaga aataaataat tctttggctg cttgggtaac attttttgtt
    10981 tcgaatactt ttttaaaata ctgcagatga agcaatttca taattatcca tcccatccat
    11041 acctttttgt tatggttttt tctttttttg gcatttttta ttatagcaga aattgactaa
    11101 gatagcagtg gaaaaagtaa gtgctgagga ggcgaggctc atgagtacag gtttcttatt
    11161 tcctggacaa ggcagtcaga agttagggat gttagcggat atccctgaaa tgtacatgga
    11221 acgtttagaa aatgtgacgg ggtatcggtt agttgataaa gaagaaaact atcaagatac
    11281 ggtttttatc cagctagcat tactgacaaa agcagtattt tatttagatg aaatggaaaa
    11341 aaacggtatt cagcctgata ttgcagcagg acattcgatt ggtgcttttt gtgcagctgt
    11401 tgcttgtcga tctttgagat ttgagaatgc agcggaacta gtctatcacc gcgcagtctt
    11461 aatgaaagaa gcttatccaa gcggttatgc aatgggcgta gtagtaggtg tgacgagaag
    11521 cgaagctgag gaaattgtag caaaaacatt tgatcctgac catccagttt atctgtcaaa
    11581 tgaaaattgt ccgatgcaac atacgttatc tgggagtatc gaaggtttat ccgttacgtt
    11641 ggcagaagcc aaacggcacc aagcgcaaac agcgaagctg ctgaaagtcc ctgtgccatc
    11701 tcactgtctc ttgatgaatg atacggtcaa gaaattcaag ccgtatatgc agtctgtcca
    11761 tctatctgat gctagatgtt tgtatataaa aaatacggat ggaagagcca ctcaggacgc
    11821 agaagagatt cgtaaagatt tgttggaaaa cctagctttc cctgtgcagt ggaaccaaat
    11881 gatggacttg gcaaaagaat taggaatgga tctgacgatt gaatttcccc ccggcaatac
    11941 tttgacaaag ctgattcata caaaatttgg tgtagataaa gggatccgta cgatcaatct
    12001 agaccaacat ggtattgatg acgccacgtt tctatatcaa aaatggagat gactaattat
    12061 ggaaaaacca aaccgcaact gggcttctaa acgcgaaaaa aaagcgcagc gattggaaaa
    12121 aatcagcaac ttgctcaatg gcaagtatgt agaaactgaa aaaatcgtag aagtaatgga
    12181 aaaactgatt gctcccggcg acaaagttgt attagaaggc gacaatcaaa aacaagcaag
    12241 ttttctttca gaaagtcttg aaaaagtaga tgcagaaaaa gtgaatgatc tgcatttgat
    12301 catgtctagt atctcacgac cagaacattt gaatattttt gaaaaaggaa tcgcaagcaa
    12361 gatcgatttc tcttatgctg gtgcacaaag cttgcgtgtc gcccaaatga ttgaagacgg
    12421 cacaatgaaa ttaggtgata tccatactta tttggaactt tatggacgct tattcatcga
    12481 tttgattcca aatgttgttt tagtagctgc agataaagca gataaagacg gcaacttgta
    12541 cacaggtttc aatacggaag aaacaccaac aatcatcgaa gctgcagctt tcaaagatgg
    12601 tatcgtgatc gttcaagtta acgaagttgt cgattcttta ccacgtgtag atatccctgg
    12661 tgactgggta gacgttgtcg tgaaagcaga taaaccttat caattagaag ctttattcac
    12721 acgagatcct caaaacatca cagaattgca aattctaatg gcaatgatgg caatcaaagg
    12781 aatctacgca gaacacggcg tacaatcttt gaatcacggt attggattca atacagcagc
    12841 tatcgaattg ttactgccaa cttatggtga atcactaggg ttaaaaggga aaatcgcgaa
    12901 gaactgggta ttgaacccac atccaacgat gattccagcc atcgaatcag gttgggttga
    12961 aagcatccat agttttggtg gcgaagttgg tatggaaaaa tatattgctg ctcgcccaga
    13021 tgtctttttt gtcggtaaag acggcacgat gcgcagtaac cgaacattat cacaagctgc
    13081 aggacaatat gccatcgata tgtttatcgg ttcaaccttg caattagatt acgaaggaaa
    13141 ctcttctacc gtaacaaaag gacgtttatc tggttttggc ggtgcgccta acatgggaca
    13201 taatcctggc ggtcgtcgtc attctacacc agcatggcaa tcacttcgcg acaatgatga
    13261 tccattaggg aaaggccaaa aattagttgt tcaaatggtt gaaacatatg gttcaaataa
    13321 aaaaccagtc tttgtcgaag aattagatgc actagctgtt caaaaacaag caggcttagc
    13381 caatgcacca gtgatgattt atagcgaaga tactacacat atcgtgacag aagaaggaat
    13441 tgcttatttg tacaaagcaa aaacaaaaga agaacgacaa gcagcaattg cagcaattgg
    13501 cggtgttaca cctcttggaa tgactagcac gaaagaatca ctagatagct tgcgtgcagc
    13561 cggcatcgtg aaattaccag aagatctagg catcaaacgt agtgaagcga aacgttcatt
    13621 attagctgca caaaccatcg atgatttgat cgaatggtca gatggcctgt atgaaccacc
    13681 aatgaaattc agaacttggt cataaaattg gcgagaaaaa ccttttgtcg ccatttaccc
    13741 agatggaaga agaaagacga ataagcagaa cgtgtatgct ctgttttaca aaaaggggag
    13801 agatccatgg aacaattagc ttataaatat ccaacgaaac agccgatcaa taagaaaatc
    13861 catgtaggag tggtcggttc aggagatttg gaaatcttga tgtttccaac agaaaaaaca
    13921 gaatctactg taaaaatttg tacaggaagc gatggcttcg gcgaagtatg gaacaatgta
    13981 ttaacgagat tttttgatcg ttatccaatt tcagcagata tcgtgatcaa tgacttcggt
    14041 gctacaccag gagttgtgta cctacgattg acgcaagcaa tggaggaact ttcagatgaa
    14101 aaatagtttt gtagaattaa acgccagaga acgagtgaac gctctacttg acaatggacg
    14161 tgaattgatt gatccattcg atcaaatcat cgcgccaagt ttaccagcac aaggaatcgt
    14221 ccctgaaagt gatgacggga tcgttgtcag ccgtggcacg atcaatggca aacaagcagt
    14281 agtaatcgct atggaaggaa aattccaagg tggcggtatc ggtgaagttt ccggtgcaaa
    14341 aatcgttgct gcacttgaac atgtgttaga agaaaataaa aaaggaaatg agatctttcc
    14401 agtcattgta ctagatacag gtggtgttcg tttgcaagaa gcaaactacg gactgttatc
    14461 tatttcagaa atcggaaatg taatcattgc tctaaaacaa tatgtaccag tcatcggttt
    14521 gattccagga cgtgtaggat catttggcgg tatgtcgatt acttcagcat taatgagtta
    14581 tttaatcgca acaaaaaaag cgagagtcgg tttgaatgga ccagaagtta ttgaacaaga
    14641 agcaggcgtt cgagaatttg attctagtga caaagacttg atttggaata cattaggatc
    14701 tcgtcaacgg gtagaatcaa aaatcattga tgaattagtg actgatagtg tagaagcaat
    14761 caaagctgct gtcatcgcag caatggacga acgaaaagac acgcatcgca gtgaaagctc
    14821 agcattttat ttatcattac ttgattcttt agatctttca aaaccaatgg ccattgacga
    14881 ttacaatgaa gcaattgcta accatcaagc aaaatcaatt gacttaccag aagtagcaga
    14941 aggaaatgaa ccagctgcag caagtgttgg ttacgattgg tttgcggcac taactggcat
    15001 gaaaaatcca acttcttcta ttagtactgt gtattatggt aaatcaagta aattcataga
    15061 agaagccatc gtgacatcaa tcgtacctga tcctaaaaac ccgatgtacc gtgtacgaaa
    15121 cggtgaagtt ggtttagttg aaggtttccg catgggacaa attttggatc acgtttacga
    15181 agaagataaa gacaaaaaag tgaaacggcc aatcatcgtt gtgatcgatg taccaagcca
    15241 agcatacggc tataacgaag aattgatcgg tatccatgtg gctttggcta atagtgctgc
    15301 tgcttatgca aaactccgtc aagctggaca tccagttatt ggattgattg caggaaatgc
    15361 tatttcaggt gcatttttag cacatggatt acaatcaagc cgcttgattg ctttaaacaa
    15421 taaagaaatt accgtccaag cgatgtctaa agaaagtgct gcacgtgtga caaaacgttc
    15481 ggttgctgaa atcgaagcag ctgctgaaaa agttccagct atcgcttacg atatcaataa
    15541 ttatacaaaa ctaggagcga tctatcattt cctagaagat atcaccgacc aagctgcttc
    15601 agaacaaaat gtcagcaaag tcatcgaagc aatcaatgaa gccattcaag atgtccgcaa
    15661 tacaaatgac acgatgctga caaatcgata cacaaacgaa acagccaaaa cattcggacg
    15721 tgtcgaaaca aataaagtcc gtgccaaaat ggacgaagaa tgggatgcat aaaaaatgat
    15781 tcaagcacat gatattgtcc tttttgataa acatgaaata gaagggttca ccttgcctga
    15841 atgggtcgtt ctaactgaaa agatgttcgg aacagttcgt cgtgcgtcga cggatcacgc
    15901 agaaatacta ccgattggtt tacgtggacc tgcccgcaat cagagatggt caacagaaat
    15961 ctcaaaacat gccgtcaaac aagtcattca tccgtgggac atcatcgaac aagaatcgta
    16021 tagacaagag aaaatcatgg attatcctgt ttatcagcaa tttcatgctg ctagaaacat
    16081 attatcagga tgtaaatggg gaatcggcgg cagcttagga tttgaattat cgactgggat
    16141 tccggcagta aaagaaacca gtgattttga tttgctgctg tacgcaaatt caccgattga
    16201 attgccaatt caggtaattc aatcgcatcc tgcttttttt gagcaattcg atacccaagt
    16261 agtgactcgg caaggcggtt tttcactaaa agagtatttg agagagccag agaaaaaaat
    16321 tctattgaaa acagtcgaag gacctaaatt aaccaaagaa ctttggtaat aaagaaaaag
    16381 agagagaaag agatgtttga aaacaaaaaa atcaaaatca acgtaagtga cttccagaaa
    16441 gtatcagctg taacggctgt tatgatgcaa acgatattga gctttgcttt ggcaaacagc
    16501 cacagcgcaa caaatacagc ttttcttggt accttttacg tcatcgttaa atacacagca
    16561 ccaatgttta tttttgcaat tgtttataat atggtaaaaa caagtcagca tttgtcttat
    16621 tgggaatttt tgaaagaaaa gttttttgaa ctagtcgttc cgtatcttct atggacgacg
    16681 gcttatcttt ggctatttcc agcagtccaa caacaaactc cgtatacgga tactgcaagc
    16741 tttttactga aatatgtcac aggtgatgga gcgcctcatt tatggtatac cgttatgatg
    16801 ctgcagatcc aattgttcat gccgtttttt gtttggctcg gttacaaagt atttgctaat
    16861 aaaaaatatg tatggccagt attgattggt tcaacgattt tatatgtcgc atggtatctg
    16921 ttctatcaaa cacaagtatt gactgggcct tatcatgaca gctggtactt attagatcgg
    16981 tttgttgcta gtttcatgat ttatggggta gccgcatttg tttatcacga aaaagtctat
    17041 caatatttgg acagagtacg ttatcttttc ttacctgttg ccttagttat cgcgttcttt
    17101 tctgtccgtt cattgctcgc acatccagga gacttgagct ttgcgaatgc accttacttg
    17161 aatacgatcc aaagtttata cagtttagtc atcatctttg ctgtcttcat aggcgcttcc
    17221 aaaatgattg taaacgattc gccaaaattg ccattgttca aatggttgtc tgtttatgct
    17281 tatcgtacgt atttagccaa tgtatttgtt ttccaagtac tgttattatt gtttaaagat
    17341 agttggctgc agttaccttc aggagtcatg attcttgtag cttatctaat gacggcaagt
    17401 tgtgcatttg ctttaagctg gttacttcat atcatttggg tagctatcaa aaaaggtttc
    17461 agcaagtaat agtaggaaga aggagtgagt gtcatgataa atgacgaacg aaaaaaagca
    17521 gtgactcagc aactcgctgc gttggtagag gaagcattga tcgcagaagt aacattaagt
    17581 ccaaaacctg gattagtgga tgcactggat gcgggtgcac attccgatat ggactatgct
    17641 ttatttctta agtctgctaa gacgctaact cctttttttg aagaaatggc tcaaatagca
    17701 tggcatcatg ctattgatca ggagttaaga gaaagaattg ctgagattgg gcgaaaagcg
    17761 gaaaaggcaa tgctgattgc tacaaatggc atcaataccc ataaaggagc gatttgggca
    17821 atggggctat tgatcagtgt cattgcacaa aaaatcagta tggaacagcc tttttcttta
    17881 tcgaaaatac ttgatgatgt cagcaagctt gctgcttttc ctgatacaaa atatcgtcat
    17941 aaaaaaacca ctcatggtca tagggtgaag caaaaatatg gcgtaaatgg tgcatatgaa
    18001 gaagcgatat tagggtatcc gcatatcagg cttgcaattc aagcagctaa ggagctagat
    18061 aatgattcat atgtacagaa acaaatgcat atgttgttag ttttgatgag ttcgctagat
    18121 gacacatgcg tattgcatcg cagtgatcag aaaacgttag tggaaatgca aaaacttgct
    18181 catgctgcta gtcaaagtgc tttaccaaat cgtccattta cagcactgtt gcacttttgt
    18241 caggaaaacc aaatctctcc tggaggaagc gcagatctct tgtctgcaag cttgttcatg
    18301 ctgaagatag aggaattatg gactgccgac acatacattt gtccgatgtt actcaatgac
    18361 taaataagac aaaattgcta aaaaaacagt ttatgcatct ggttattgac tcttcctttt
    18421 agcaaggcta tcataaaaat agttttttgt aataaaagat aacgaggaag acaatgaaaa
    18481 aaatcaatta tttagcaatc attttgttag tgggcctatg tttgtttgct ctgccgaccc
    18541 aaggattcgc tgtcgcgggt ggtcatggtg gaggtacatc tagcggtcga agttcgggca
    18601 gaggttcatt tggtggtcgt tcatatgggg gcagttattt tggcagtcgt tcttacggca
    18661 gagattctta tgggtattat ggtccaggta caggtccttc tagttatgag caaacgggat
    18721 ttcttgcatt agctgttttg tcagtaggag gggtttctgc tttttctatc gtcaagaata
    18781 aaagacaaaa gaaaaggctg aaagcggaga tattgtcccg catgtcagga acggctaaag
    18841 aaaagaaagc acagtttctg gaaatccaaa agatattcat gaagatccaa tccgcttggg
    18901 aagaagaaac tcctgaaaaa gccagaagct gttatacaga caagctgttt gccgaacatc
    18961 agaaagttct gcaaaaaaac aaagcagacg gtgtaagaaa tcatactaaa aaaataacta
    19021 ttcaggaagt aggaaattat cgacagatcc acgaaaatag ttttgccatc cgaattgatt
    19081 tcagatgctg tgattattca gtcgatcgaa aagatgggcg attgatctca ggaagtaaga
    19141 cacaacgaca gtatttttct cagatatggt attttgattt ttctgagaaa gaaaaaagat
    19201 ggcaagccga ttttattcag ccgattatgc tggattgaca ggaaatattt atgaacggag
    19261 ttgtgacaaa agccttgtcg cagctccttt ttctgaataa acggtgtgcc agagatagcc
    19321 tatcggcaat aaggcacgat tttgtaaaat atgggaagcg atttttcaaa atcgcttctt
    19381 atcgctcaag gctgaacatc tctgtcacaa cctctccact aaatggtgtt taaaagcttg
    19441 catctgcgat aataagaaca accaaacgaa aaataggaag cactatttta gtttagttgt
    19501 tcttattact tgatgcagga cagctttttc acaacctctt ttttgtatag atgggcaaaa
    19561 tttttgtaat aataggcgta tgtctttttc gttgaggaga tgaaaaagca tatggcttat
    19621 tttttgtttg ctttgcttac tttctttcca cttcaagtgg cagaaatatc tactttttat
    19681 tatgtaagcg gaaccatata cttgaaatca gaaggagatc atttctatga aaacaagaac
    19741 aattgaaata cttcacagaa tcatgacagc ccaatatcca gtaaaagcaa aacatctagc
    19801 aagtgagttc aatgtcagtc agcggacgat ccgtcaggaa gttcttgaag cgaatatctg
    19861 gttgagcagt aaaaagcttc cggagatccg tacaattcgc aacaaaggtt ttttgttgaa
    19921 gctggaaaag gaaaagcaag ctcgattaga aactgaaata aaagatgtga aagacggctt
    19981 tttgaatcgg cgcgaacgta tttttgatct agttttagcc gttgcttaca gccgaatccc
    20041 gatccatcta aacaaaaaag aagaagaatt tcaaatctct aaaagtacat tagatgaaga
    20101 tatgagacgg ttacgagcaa cactggtcag ttatgggata gagatcgtca gttatggaaa
    20161 gcaaggactg gtttataaag gttcagaacg atctatccgt acgatgatct atgattttat
    20221 caatcagaat ctgggaatga ccgacttctt tatgaatcaa gatgcacaat taacaccggc
    20281 tcaaaaaatc tttctccatt attttccttt ggtggaaatc aaaaaaatac aggagatcta
    20341 ttccaataag ttgttgaaac aagaagatga tatttataaa aaacaaatcc tgctttttac
    20401 attgatctgg atccagcgaa tcaggaaaca tgaatttatc tctgccatta attggaaaaa
    20461 tacagaaata aaagacagta gcttttctgc atttattgac cacatgatcg aggaatttca
    20521 attggacaat gtcccgcaag tagaaagaaa ttatcttcgt ttcactattg agacgttcaa
    20581 tgctagagat atctccaatt caatcgagtg ggtacaagct caattactaa cgatccaatt
    20641 gatccaattt gtagagaacg agacacacgt acctttccat ctaaaggaag aagctttatt
    20701 cgaaaatctc tataagcaca tggctgcact gattgtaaga atcaagaatc atatccaagt
    20761 catgaatcca ttgaaagaaa acatccgaac aaattatttc ccaatctacc gcgcagtcac
    20821 tcattttgtt cctacgatcg aagaagtaat tggtggcaag atactggagg acgagatagc
    20881 ttttcttgtc atccactttt cgacgatcgc cagtgtaatc aagcgagact tgaattatgt
    20941 ttataaatca gtcgttgtct gtaaccatgg gatggctact ggaaatctgt tagcagagaa
    21001 tctgaaagaa aagtttcctc agatcgagat tactgcagtt ttaagttcaa aagaaatcga
    21061 attagtagat aaattggatg tcaatctggt tttcagcacc tttcaattga attatcgaaa
    21121 taagccgctt ttagttgtcg atccgatttt aactgagacg aatatcttgg ttatagaaga
    21181 ctttttgaaa agaaatgaag aactacagcg gttagagcct aacagtcatg aatcgacgga
    21241 gttattcact caaataatag atgtcatgaa agaaagtggt ggccgagtag atcgaatcat
    21301 ctatggcaag ttagaaaaat tattcgaaac aaacaattta gaaatcaaca aaagggagat
    21361 ccagcctatg ttaaaagata ttttgacgga cagcaatatc atgatcaaag aaaaagccac
    21421 tacttgggag gaatcgattg aactagctgc tagaccattg gttaaagaaa acattgtcga
    21481 agagcgctat gtcgaagcga tgattcgtgc agtgaatgat tacggaccgt atatcgtgat
    21541 tggcaaacac atggcactag cccatgcccg accagaagat ggggtcaata aattaggagt
    21601 cagcgtagct acgattgagc aaccaatcga tttcggtaat cctgaaatgg atccagtaaa
    21661 gattattttt tgtttggcag cagtagactc ctactcgcat ctgaacatca tgaaagaact
    21721 aattgaattg atcaacaacg aagagaagtt ggatcgattg attgaatgca caaatatcga
    21781 agatttcaaa caactgcttt tttcagtagt tgagaaataa taaatgatac gggggagaac
    21841 aaagatgaac aaattgaaca ttttattcgt atgcggtgca ggtttaggaa gtagttttgc
    21901 agcacagatg tctgctgagg atgttttgaa caaattagga gtagaagcca aactggacca
    21961 ttgtgatatt tcttccgcag tttccatgaa accagatgtg atcatcacag cagaaaattt
    22021 tcgttcgcag ttcgaaaagt tcgcagttga tcctaaaacg gcgatgatct atttgaaaaa
    22081 tattgtttca aaaaatgaaa ttgaagaaaa aatcacccca gttctgcaag ataagggagt
    22141 tatctaaata caaaaagaaa agagggtaaa aa
//
LOCUS       DAZKOM010000041        21979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000041 DAZKOM010000000
VERSION     DAZKOM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.041"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8NF_001491"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8NF_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0471979.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8NF_001492"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8NF_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0471980.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8NF_001493"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8NF_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0471981.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8NF_001494"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8NF_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0471982.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG8NF_001495"
     CDS             complement(4333..4647)
                     /locus_tag="ACG8NF_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471983.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8NF_001496"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8NF_001496"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0471984.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8NF_001497"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8NF_001497"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0471985.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG8NF_001498"
     CDS             complement(5984..6916)
                     /locus_tag="ACG8NF_001498"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0471986.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG8NF_001499"
     CDS             complement(6913..7689)
                     /locus_tag="ACG8NF_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0471987.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8NF_001500"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8NF_001500"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0471988.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG8NF_001501"
     CDS             complement(10933..12009)
                     /locus_tag="ACG8NF_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0471989.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG8NF_001502"
     CDS             complement(12014..13294)
                     /locus_tag="ACG8NF_001502"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0471990.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG8NF_001503"
     CDS             complement(13313..14239)
                     /locus_tag="ACG8NF_001503"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0471991.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG8NF_001504"
     CDS             complement(14252..15535)
                     /locus_tag="ACG8NF_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0471992.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8NF_001505"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8NF_001505"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0471993.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG8NF_001506"
     CDS             complement(16323..17234)
                     /locus_tag="ACG8NF_001506"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0471994.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8NF_001507"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8NF_001507"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0471995.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG8NF_001508"
     CDS             complement(17700..18398)
                     /locus_tag="ACG8NF_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0471996.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG8NF_001509"
     CDS             complement(18385..18771)
                     /locus_tag="ACG8NF_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0471997.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG8NF_001510"
     CDS             complement(18918..20588)
                     /locus_tag="ACG8NF_001510"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0471998.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG8NF_001511"
     CDS             20771..21004
                     /locus_tag="ACG8NF_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0471999.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG8NF_001512"
     CDS             complement(21103..21534)
                     /locus_tag="ACG8NF_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0472000.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>21979
                     /locus_tag="ACG8NF_001513"
     CDS             21613..>21979
                     /locus_tag="ACG8NF_001513"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0472001.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNL"
BASE COUNT         6208 a         4914 c         3884 g         6973 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttg
//
LOCUS       DAZKOM010000042        21856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000042 DAZKOM010000000
VERSION     DAZKOM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.042"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8NF_001514"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8NF_001514"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0472002.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8NF_001515"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8NF_001515"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0472003.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8NF_001516"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8NF_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0472004.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8NF_001517"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8NF_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0472005.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8NF_001518"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8NF_001518"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0472006.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8NF_001519"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8NF_001519"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0472007.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACG8NF_001520"
     CDS             complement(8054..8524)
                     /locus_tag="ACG8NF_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0472008.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACG8NF_001521"
     CDS             complement(8570..8995)
                     /locus_tag="ACG8NF_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0472009.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACG8NF_001522"
     CDS             complement(9062..9331)
                     /locus_tag="ACG8NF_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0472010.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACG8NF_001523"
     CDS             complement(9342..10937)
                     /locus_tag="ACG8NF_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0472011.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8NF_001524"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8NF_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0472012.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8NF_001525"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8NF_001525"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0472013.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACG8NF_001526"
     CDS             15403..16377
                     /locus_tag="ACG8NF_001526"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0472014.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACG8NF_001527"
     CDS             16547..17947
                     /locus_tag="ACG8NF_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0472015.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACG8NF_001528"
     CDS             complement(18025..18747)
                     /locus_tag="ACG8NF_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0472016.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACG8NF_001529"
     CDS             complement(18826..19332)
                     /locus_tag="ACG8NF_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0472017.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACG8NF_001530"
     CDS             19455..21254
                     /locus_tag="ACG8NF_001530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0472018.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..>21856)
                     /locus_tag="ACG8NF_001531"
     CDS             complement(21322..>21856)
                     /locus_tag="ACG8NF_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0472019.1"
                     /translation="TTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPT
                     PEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVT
                     GRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGES
                     VKVGSVQWFAPDFAVSM"
BASE COUNT         6054 a         4794 c         3970 g         7038 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgtt
//
LOCUS       DAZKOM010000043        20754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000043 DAZKOM010000000
VERSION     DAZKOM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.043"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..1016)
                     /locus_tag="ACG8NF_001532"
     CDS             complement(129..1016)
                     /locus_tag="ACG8NF_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0472020.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1201..2040
                     /locus_tag="ACG8NF_001533"
     CDS             1201..2040
                     /locus_tag="ACG8NF_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0472021.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2236..3210
                     /gene="sgrA"
                     /locus_tag="ACG8NF_001534"
     CDS             2236..3210
                     /gene="sgrA"
                     /locus_tag="ACG8NF_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0472022.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3508..3864
                     /locus_tag="ACG8NF_001535"
     CDS             3508..3864
                     /locus_tag="ACG8NF_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472023.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3909..5024
                     /locus_tag="ACG8NF_001536"
     CDS             3909..5024
                     /locus_tag="ACG8NF_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472024.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5050..5490)
                     /locus_tag="ACG8NF_001537"
     CDS             complement(5050..5490)
                     /locus_tag="ACG8NF_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0472025.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5650..6156
                     /locus_tag="ACG8NF_001538"
     CDS             5650..6156
                     /locus_tag="ACG8NF_001538"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0472026.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6324..8237
                     /locus_tag="ACG8NF_001539"
     CDS             6324..8237
                     /locus_tag="ACG8NF_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0472027.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8242..9243
                     /locus_tag="ACG8NF_001540"
     CDS             8242..9243
                     /locus_tag="ACG8NF_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0472028.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9445..9651)
                     /locus_tag="ACG8NF_001541"
     CDS             complement(9445..9651)
                     /locus_tag="ACG8NF_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0472029.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10297..11145)
                     /locus_tag="ACG8NF_001542"
     CDS             complement(10297..11145)
                     /locus_tag="ACG8NF_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0472030.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11309..12211
                     /gene="gnd"
                     /locus_tag="ACG8NF_001543"
     CDS             11309..12211
                     /gene="gnd"
                     /locus_tag="ACG8NF_001543"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0472031.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12213..13214
                     /locus_tag="ACG8NF_001544"
     CDS             12213..13214
                     /locus_tag="ACG8NF_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0472032.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13211..13495
                     /locus_tag="ACG8NF_001545"
     CDS             13211..13495
                     /locus_tag="ACG8NF_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0472033.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13528..14325
                     /locus_tag="ACG8NF_001546"
     CDS             13528..14325
                     /locus_tag="ACG8NF_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0472034.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14339..15172
                     /locus_tag="ACG8NF_001547"
     CDS             14339..15172
                     /locus_tag="ACG8NF_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0472035.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15190..16047
                     /locus_tag="ACG8NF_001548"
     CDS             15190..16047
                     /locus_tag="ACG8NF_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0472036.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16069..16578
                     /locus_tag="ACG8NF_001549"
     CDS             16069..16578
                     /locus_tag="ACG8NF_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0472037.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16615..17016
                     /locus_tag="ACG8NF_001550"
     CDS             16615..17016
                     /locus_tag="ACG8NF_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472038.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17128..17481
                     /locus_tag="ACG8NF_001551"
     CDS             17128..17481
                     /locus_tag="ACG8NF_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472039.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17515..17958
                     /locus_tag="ACG8NF_001552"
     CDS             17515..17958
                     /locus_tag="ACG8NF_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0472040.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            17975..19060
                     /gene="uxuA"
                     /locus_tag="ACG8NF_001553"
     CDS             17975..19060
                     /gene="uxuA"
                     /locus_tag="ACG8NF_001553"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0472041.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19053..19766
                     /locus_tag="ACG8NF_001554"
     CDS             19053..19766
                     /locus_tag="ACG8NF_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0472042.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(19973..20122)
                     /locus_tag="ACG8NF_001555"
     CDS             complement(19973..20122)
                     /locus_tag="ACG8NF_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472043.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            20518..20718
                     /locus_tag="ACG8NF_001556"
     CDS             20518..20718
                     /locus_tag="ACG8NF_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0472044.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7098 a         3204 c         4314 g         6138 t
ORIGIN      
        1 ttgttttggt acaaataaaa aaaggccccg atgaagttgt ggcaaaagtg gtttgcctca
       61 aaaataagaa ggaatttcca aaaatcactt ctcaaatttt gccacatccc caattgggtc
      121 aaatttgttt atttgaatat attttttaca taaggatctt taatagttac attgtctttt
      181 ttcatcactt tacggattgt tttatccatg aattccgtat caagagtttt tgcttcggta
      241 gcaatttttt ctagctcaga tttgtatttg tctttattag aacctttatc ctgttttttt
      301 accattttta ctagataata gctttgctga taagtcgttg gatctgttac aggaatgata
      361 tcagaaactt ccccgttttt tagtttaaat gctgcttttt ttacttcagt aggaatctct
      421 ggattggttg aatcaaaatt catttttcca tcagtttcct ttaatgtttt attttttcca
      481 taagcttgaa caagcttgga gaaattttct ccttcatttg cttctttttt tgctttatct
      541 gcttcttctt tagtcgaaaa tagaatgatc tgtgtactaa cttctggata gaattctttc
      601 caagcagtgt ctagatcttt atctgttaat tttacattgg ctttcaaacc gtattggaaa
      661 gccagcatct gtttaaagag tttcttatat gtctcttctg tttggccaac tgcttttaat
      721 tgttccttgt agtttggacc gagttttttg atttgttcat tatattcttt tgttacttcc
      781 tttgaagaaa tctcttttcc gtatacatca tcaaatgctt tgaagacaat catttttttt
      841 aatagtgttt gattatttga tttaactttt gggttatcat aaaaatcctg aactgtaagt
      901 gttccacctt taaacgttgc aatcgtttca ttggacttgc tacaacctga gaatagtaaa
      961 attgcacatg ctgaaaggca cataattagt agttgttttt tcaaaaaatt tcccatcctt
     1021 tccttattaa aaacagtcta acagaagaat tagggagaca gtctttcatt ttcgtaaggt
     1081 tgctgtagat attcttagtt cgtaaaaatc tattctctct atgatggaga acatttacat
     1141 agttttcacg agacaagaaa gaaaagggtt gttaaactta atataaagga gggatgaaag
     1201 atgatcagga ttattcggtt gattggatct gttttaatag tcttagttgg ttttgtttta
     1261 ggaattcttg tcaataatgc acttgcggat atgtttattg gaaatagtga atggattgga
     1321 gcaattgccg gtacggtcgg acatttagct gtttttttac tagctttatt tcttgctagt
     1381 aaggtcgaag gaaaaagaat agaagattat ggtgtttctg ttcgaggaaa agactggggg
     1441 tatttagcgg gaggtctcgt tgttggaatt ggagtgtttc tgcttattac tagtccatta
     1501 tatttgacgg gggcgtatcg gttagattca ggaaacgcaa acattgtgcc gctgataacg
     1561 agttttattt tatttattgc agtgggggca tcagaagagc ttttattccg tggctttttt
     1621 cagcatcaga tgcttagatt tggtccgtta atagcaatga ttggatctgc tgcattattt
     1681 gctcttttgc acggattgaa tcctaacatg acatttctgg cagttttcaa tatcttttta
     1741 gcaggttgct ttttcagcgc acttatctac agtacaggaa gtttatttac ggctattggt
     1801 gctcatatta cttggaattg gacacaagga gctattttgg gtataccagt cagcggaacg
     1861 caagaacaag gttttttctc cactctgatc caatcgcaaa atacaataat tactggggga
     1921 aactttgggg ctgaagcatc tatatcaact acattattct tgttagtctt gattgttggt
     1981 ttgttactct atgcatataa aacaaagcgg atgaagaaat acactgctgt ttatcaataa
     2041 aagatgaaga aagcaaggct gatatcggtc ttgctttttt aagtctgtca ttgattaaac
     2101 gaaaaagtaa acaaattcaa gcgaatacac gacatctttt gttagatttt cacaactgac
     2161 tgtcgtgttc tcatcatttt cattttatat aattccttta tcaaatagaa ggtaattata
     2221 tggagagtga tagaaatgaa gaaaacagcg accgtattat ttgttattct tgggctgagt
     2281 ataattgcag caatgattgg aatcagtact tctgcaaatg aacgggcaaa tgagaatctt
     2341 tcatttactg tgaaaactga tcggattgtt tatgacatga cacaacaagt cattactatt
     2401 ccagtcaaac caaacaaaag cgtgaatgct agtgatgtac atgctgttct aacatatggt
     2461 tgggatggta atggaagttc agaaaaagtt attggggaag tttatttaaa agacgtacaa
     2521 tggaccgctg gtattgagta tacaataatg atttctgcag aactatcgat tgatgagatc
     2581 aagagtaaag ataaagttga tttgattgtt ttttatgatg gacagatgac gatcacggag
     2641 aatttgaaac caagcagttg gacagttgtt ggaccgccaa gcagtacgga tagcacaagt
     2701 agtgaaagca gtacagaaaa cacgagcggt gaaagcagta cagagagtac gagcagtgaa
     2761 agtagcacag aaaacacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     2821 acaggaagca cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga
     2881 agcatgagta gtgagagtag cacaggaagc acgagcagcg aaagcagcac aggaaaagta
     2941 aataatgaac ttgatttggt acctatcata ccaactaagg aacaaaagag aaatgcaggg
     3001 agcaacctaa aaagtgtgct aaaacagcct gattttgttt ctcgtggaat tgatcatcaa
     3061 gaaaaagcga aagaaacaga taatagaaaa agcgatttac cagaaactgg tagtagaacc
     3121 ttgaacaagc taattacatg gatgggtgtg ttactcattt tgattgtagg agcaagctat
     3181 tttcgttctc tcttccatcg agtgaaataa agaataaaag caacagatct aaaattgaat
     3241 aaaacgatag atttttgagt gaatatttca ttcgagagtg ctgtattgcc tggcgaagtt
     3301 gtagctttgc cagatctatt tttgtttact aacatatttg agaaacagga aataacagga
     3361 acacaaatgg aaaataagag caagaataat tgacaatgca cactgcgcat ggtagtatct
     3421 ctgaaaaatc agtaacgaat ctatttgtac gttattcatg tagctgtcgg aataagtcta
     3481 aataatagat aaaaaggagt atgtactttg tttgaagaat ttattgatat caatgaaagg
     3541 caagtatatc aatttttgaa ctattgttat gaaagagacg agaaattata tgtggtgaag
     3601 gatatcgctt tagatctaaa ctacaccctg gctaagatga attcggtgat tcaacaagca
     3661 gaatcgtttt gcgaaaggta tccagaatat aaattatctt ttctttcaga aaacaagatg
     3721 ataaaagtcg agttttcttc tcagttttta ctgagtaaag tttattctat acttttagag
     3781 ggaacgattg gttatatttt actggattca ctttacaaag gtacttatca atccttagaa
     3841 aatcttagtc aaaaaattat ataagtttgc gtacgattca aagaaaatta agagaactta
     3901 atactatttt gaaaaactat catttatctt gcagcttgaa aaaaggtaat ccgatagaag
     3961 gagaagaata ccgtatccga tacttttttc acttgatgta ttggcagtca ttcgacgaaa
     4021 atcattctga tatattatca agtataaaaa ataaacgtca actattagaa aaatttgcga
     4081 aacatgcgcc atatacgcgt agtatagata gaaagaaatg gatcagccta ctaagaattt
     4141 cgttgcaacg aatgaaacaa ggccactcga ttacgtatgt cccagaagag atgaacaaat
     4201 ttgtgcatcc attgataaca aaagaagagt ttacgcatta catactagag cctttttttg
     4261 aagcaaactt ctggtcgatg aatcacatca ctccaacaga gcaagcttac ctttatttta
     4321 tgtttggtgt aatgaacacc tatttggaag aagatttgtt ggatactcca gataaagtac
     4381 aagaaagttg gggctggatt gaatcgtcta ttgtagaaaa ttttgaaaaa aacttcaaga
     4441 tttgttttac aacttcagaa aaaaattatc tatatgtcaa tttggcaatg atccatttgt
     4501 attcaagggt ttttgggaca aaaaagaagg tggatgcttt tggaaaatca gcagaagaca
     4561 gagattttca gcatatattt ccagtgatgt atccaatcat gcaacagttt tttagtgggg
     4621 tagctcgcag aattccagag cttaatactt attatgaaaa gaacagacgg ttgatttttc
     4681 aatactgtat gttagtcagg gatattttta taaaacacga acaacctgtc atattctata
     4741 ttcaatcaaa gtatggtaaa acacaggaat tatgggcaaa gaaaaggata ttatctatga
     4801 ctgaacgccc aataaaatat agtgcatccg ctgatcagtt acctgatatt gtcattagcg
     4861 attatcctat cgacaaaaag gcatatgatc aaacgaatac acagatattt tactggaatg
     4921 gacaacctac aaaaaatgat tgggcgcgtc tgaaagctga aatcataaaa atcaggaatg
     4981 agaaaaatag atccatattg gaaaaattgg atttcgttaa ttaagaaaag cagattgttt
     5041 atgccaaaat taagacattg caaaaagatt attttcttta aaatcgacaa gatgaccaga
     5101 aagcaagagt tcgttgagct cttgctttct tttagtttta gaaaggaaca tcctgatttt
     5161 atcttcgcta aatccagtca aaagttttgc agattggtcg tagataatcg gttttttcaa
     5221 taaagaaggg gatgtacgaa tctcaatata tagttcactc aaactgagtt catctattct
     5281 gtgaacaaga tttttataac taatagaatg agtagagagc atttcggtca cgccgttgtt
     5341 tgtcaatttt agtaagtgga taaattcatt tttggttagt ggttgacgaa ttaagttctg
     5401 ttcgataaac ggtatatcat ggacgataag ccaacgtttt gcgcgtcttg aagacgagca
     5461 gccagaacct gtatacaatt taatcatcat acattcctcc aatagctaag tatttatgaa
     5521 ataataacat gaagaaatag tcgaaaagtg ccatttagag gtgtaaaata agaaaatgtt
     5581 gtcacgtata atggaactag ctagataaga aatactatta aatcaagaaa aaagaaagaa
     5641 ggcacaagta tgtcaaacac agccgacgca atagtagtga tcgatttaca aaaaggggta
     5701 tgttttgatt cttcgccgat acacgaatta gaaacagtga cagcattagt gaataaaaga
     5761 atcaaaacat acgaaacgat tgggaaacca gttattttcg ttcaacacga agatgctcaa
     5821 ttagtgccac aaacaaaacc atgggagcta ttgtcggaat tatctattcc tgagcacagt
     5881 tattttgtga gaaaaaccca tgcgaattca ttttataaga ctaatttgag tgttttatta
     5941 actgaattgg actgtcattc gatcgaatta tgtggggcac aagcagagta ttgtatagat
     6001 acgactgtga agtttgctca tggattagga taccagctta tgatgtgtag aggagcaacg
     6061 acaacttatg ataatccttt tatgaaagca aaggaaacaa tcgcttttta cgagaaaatt
     6121 tgggataaac gatttctgac atttttggag caataagttg tttcttcgtt taaaaaaagc
     6181 agaatggata ctggtttatc cattctgctt tattacgtaa aatgagaaaa taatcctgtt
     6241 ctatttttat gggataaacc atttattttt tgtataaaaa atgtttgcgt ttacatataa
     6301 ttctcctaga aacataaaaa ggagtgaata gcttgagaac aaaacaagta ttaaagggag
     6361 cgttggtcat cttatttgct gtaagtacaa gcgtcccgca atggacagta tatggagaga
     6421 gcatttcttc cgaatcggaa tctcgggagg tagaaaccac agaaactact gatgctggaa
     6481 atgaaacgaa atcgagtact tcacaaacag aaacaaatac tgaaacaacg gttgagacat
     6541 ctgttactag cacagatcaa gaaacgaccg ctaccacaga taaccaacaa acagaagaag
     6601 caacagctac aaaaatgtca aacgacgaat cctcttctgc taaagtaagt gacaaagaga
     6661 atgggacaga aaatgatgat atcggtaaag gtgaaaattt caatggtaaa gaatatttga
     6721 attacttcgg taaacagatg acagaaatac ttaatggacc aacagaagac atcaagtcaa
     6781 aattaataga gttattagtc actgagcttg ctgttaatga ttatggtgta gataaagaga
     6841 ttatcaaaca tctgattgct acttatctat tgtacgatat cgcttttgca aaaaacagtg
     6901 tgttgatgtc gatgcttgga ggtacagatg atgggacaac gcctacagaa aacacaggct
     6961 tctatccaaa tacaaaacaa cttcatcaaa ctgtactaaa caaacaagat ggcaaaatga
     7021 tggatgtata tgcatattat gtcgatcaag gttcagataa aacagtcatg atccatggcg
     7081 gatttcgagg gaattggaat aacgggatcg ttacagaaga atacaatgat ttttataagg
     7141 ccgggtataa tttattattt gttgattcca gagcgactgg taatagcgga ggcgattatg
     7201 tgacctatgg acaatatgag tcagatgacg tcctttactg gataaatcaa gaagttcgag
     7261 agcggccatc tcaaaagatt ttactttatg gtggttcgat gggagcagct acgatgatgt
     7321 ctgtattagc gaaagatatt cctgtcaatg tcaaaggaat tattgaaaat tgcggatttg
     7381 catcaattga tgaacaacta cgttttacgt acagccagac agtagccccc gcactaccgg
     7441 atgccataaa aaatcagtta gacatcattg gggatcaaga gcatgaagat ttatttatgg
     7501 gccttttgaa acaatactat tttgatcagg agatgcattt ggatacaaaa gctgctctac
     7561 ctacgattgg aatgtcagat tcattgccta aattgattat ccatggaaca gcagatgatg
     7621 tagtacctgt gtctaacgcg caaaaattat atgagttatc aggagggtat aaagatcttt
     7681 tattagtaga aggagcaggg catggaaagg cacaggaagt tgaccatgct gcctacacga
     7741 aacatgtaac ggactttttg aaagtagtgt tccatgatca gataaacgta aagtatgtgg
     7801 atgaaaacaa taaaagtcta ctcaaagacc aggacgaaat ccagctttac ggagcttatg
     7861 gagaaaatta tatgacagag caaaagacat tcgaaggata cgaattagca aatgttgaag
     7921 gaccgactga agggattttt aatgagacga taccaactat tattttctat tacaaaaaaa
     7981 tcccagtagc tccaccaaaa aagcaagatc cgacagagaa tgcagacaat aagggaaaga
     8041 atgaacctcg agaggatcaa tcaagtgatg atacgcaacc gattcagatg acagtgaaag
     8101 tacaaaaaaa gacagatgca aaaaaaagaa tgttgccgaa aactggagaa aagaaacatt
     8161 cgcttctcat ttattcaggt ggatttttat ctgttagcac gattatcaca tggatttggc
     8221 gaagaaagag agggtaaatg aatgaaatgg ttcaagcgaa tagtgattgc agtaccagtt
     8281 atcatcgtag ttattttagt cactgtattt ttgattaatc aaatcacacc aaagccgatt
     8341 agcttgcttg ttagaaaaca atttgacaca agtgaaaaag aacaagtata tgcccgacca
     8401 gatgatttcc agcaaaaaat cgaacaaatc gatataaaaa aagaaatatc ctatccttca
     8461 aagtacaaaa ataataatat ggatatctac acaccgaaag tggaaaagaa aaaattaccg
     8521 attcttttct ggatgcatgg aggcgcatat gtaggagggg ataaaaatga ttgtcgtgat
     8581 tatttggaat atctatgttc agacacgcag caaatcatcg taaatataga ttatgaacga
     8641 tcaccggaag caaaacatcc tacaccagtt atccaattga acgaagccat tcaagcagct
     8701 aaaaaagaga taaaagatca agcagattgg tcgaatattt cgattggtgg aaattcggca
     8761 ggtgcacaaa ttagtggaga atatttgtta gcacttcaaa atgaagaaat taaaaaagct
     8821 gatcatcttg atccgacttt ggagaacaag cagattacga agtttatttc tttgtctggt
     8881 ctgttggatc cttctgcact tactcaagta agtgataaaa tctctagctt tctttatgaa
     8941 aaatgtggat gggcttactt tgataataag gactttgaac aatcagagaa ggtcaaaagt
     9001 ttagccttgg tcagacatgc ggatcgttgg acgcaaaaca cattcttaac ggatggaaat
     9061 acaaatacgt ttacgaaaca aatggaagaa gtaacttctg tatttgagaa agcgggggtg
     9121 aaagtcacaa aagtagatta tgcggagaaa aatgctgttt tgaaccatga atatcagttt
     9181 gatttcagca ataaacaagc gcaagaaaca tatcaaaagt tagtaatgtt tttcaaggag
     9241 taaagtctta tgatgcaaaa gaggttgtaa tctgtcactt agctccaagt cacaaggagc
     9301 aatttcaata aacaagcttt tcatggttta tgaatattgt aacttgtgac tgaggagtta
     9361 gagatggcaa ggggcacctt ttattcgaaa tttgggaata tcacacgatt attgtcacga
     9421 tctcttttac gtaaatttta taagtcagat ttgttgtttt gatcgtttaa aaaaagcaac
     9481 aaatccgaag cctgcggagg caagaaggga caaaaagccg agtggtaagc aaaagaatgc
     9541 tggttccact aatattccgt tgttattgat ataaggctgg gaacaaagat gaatagcaaa
     9601 taaaatcaat gcactagcga aaaaaagcaa agaaatatgg atagctttca taagcagcac
     9661 ctcctttttt ataattgtat gataaataga ttaagaatct ataaatgtga caagtttgtt
     9721 aggatgtgaa taaaatgatc atctagcgaa cttgaaacaa agatggctaa tgataagcaa
     9781 ttaatgattc agcaatcaga taggtacaaa gtttaccatc atacgtctag accaataata
     9841 gaaaatatgg gaatataata gaaaagattt aggatacccc caaaagaatt tccccaaaaa
     9901 ttcgatgtat cctaaacttt tttattttac ataacaatca tatcaactgt gaaataaaat
     9961 tacaaatgat aacctaaaat gaggccaaaa gccagtgtta tcaaggaaat acttaattca
    10021 ccaaaatttt ttttaatttt ttagaaaata agaaacaaaa ataataaaaa aaagaaaaga
    10081 taaatttttt actactgtat actaactttg ttatcgcatc taacaagtaa ctcgaataat
    10141 accaatacaa ccttctccaa agatgcgatg acatacgttc taacccaccg actattgatg
    10201 ttctgtacca cccttgcaga accaaaaaag ccactaccaa atatttggta gtggcttttt
    10261 ttgttttatg gatttttcgc tttttctgtt atggtgctat tctttgattt cagaaatagt
    10321 atgtcgaact ttagcgatag aacgacgtga ttctttttcg ttgtaaaaca tcaaaatcac
    10381 atatagagag tccaaaatgc ttagttgaga aatacgggaa gctaaagctt cagatcggaa
    10441 ttctgtttct tcagaaatag aaataaaaac gacatcgcca agtttagcaa gggaagaatt
    10501 agcttggcta gtaataacga ttgtttttgc tccagttttt cttacggttt cagcaatatg
    10561 gattgtttct tttgattgcc ctgtgtgaga aatcaaaaca gcacagtctt tttccgtcaa
    10621 taaagaagct tccatcaatt gaatatggta atccgtgctg tgagaagttg cgatagggga
    10681 acgcaaaaat ttatgataag cgtcagttgc tacaatctct gaaccgccaa caccgaaaaa
    10741 atagacacgt ttagattcat tgataatagt agccgctttt tttagatcct tttgttgtaa
    10801 caactttttt gtgtcattta aagttgaaat actagactcg aacacctttt gcgccatagt
    10861 gagctcagta tctgttttct gaatattttc gtggatagaa atagcagata gatcattttc
    10921 ttggatcaac atggccattt taaaatcttt aaatccatta taaccaagtt tttttgtaaa
    10981 ttgaaaaaaa gtagagtcag caatgcccag ttcacttgct aaattgctaa taggactatg
    11041 acttactttg cttggatttt ctaaaatata atcggcgata gcctgttctt ttgggctgaa
    11101 gtcgttgtac attgttttga tttgtagtct tatggagacg ctcatgagaa ttcctcattt
    11161 tcataataag attatcattt tttccgttct cattaatgtt aaagatacaa ggagagtagg
    11221 aaaaaatcaa acaaaaaata tttttgtaaa atctatttac aaaaaaattt ttttgtataa
    11281 tgagggtata agaaatggag gcgctatcat ggaaattggt gtaattggtt tgggaaaaat
    11341 gggattgaat ttagctttaa acttaaaaga gcaccatcac aaagtacaag gactcgacat
    11401 ttcaagtaca gctgttagtc aggcaagaga aaatgacatt cctgcttatc aagagatctc
    11461 tgaatttcta gctacttttg agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga
    11521 gataaccgaa aacatgttaa gaaaactttt tccaaaactc ggaccaggag acattattat
    11581 tgaaggcgga aactcttatt ataaagattc aatccgtcga gcagaagagt ttcagaaaaa
    11641 tgaaatcggt tacttggatt gtgggacttc tggaggaatc gaaggagcac gacatcaagc
    11701 ctgcttaatg attggaggag ataaagaaac ttttctatcc gtggaacaat tattcaaaga
    11761 tgtagcaatt gaaagaggat atctgtatgc tggtgcatcc ggaagcggac acttcttgaa
    11821 aatgatccat aatggaatcg aatatggaat gatgcaagca atcggtgaag gattccaact
    11881 tgtacaaaaa agtgagtatg cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg
    11941 ttcggtgatt cgcggctggc tgatggaaat catggaagat cagttaaacg aacatcccaa
    12001 tttaagtaat tatcggggaa tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac
    12061 agcgctagaa atggctgttc cagttccagt aattgcaact agtttgttca tgaggaatct
    12121 ttcacaggaa gaagatagtt tttcagcaaa agtagtttca gcactaagaa atgggtttgg
    12181 cggacatgaa atagttgaaa agggtgagta aaatgccaga ttatcatata gcaatcgtta
    12241 attccagcag tttcggaaga gtctttccac accacttgga aagattaaag tcagttggag
    12301 acgttcattt ctttactgtt gatcaagaga ttccaggaaa agaactagcg gagttactta
    12361 aaggattcaa tatcattatt gcaagtgtta cgccattttt tcgacgagat ttttttgaaa
    12421 acaaagatga actgctattg atttcccgac atggtattgg ttacaacaat attgatttaa
    12481 aagcagcaaa agaacaccag acaatcgtat ctatcatacc tgctttagtt gaacgaaatg
    12541 ctgtagcaga aaataatata actaatttgt tgtccatgct tcgacgcacg acacaagcac
    12601 aagcaagagt caaggacgat cgatgggaag atcgggcgga gttcgtcggt aggacgctgt
    12661 ttaacaaaac agttggtgtg attggtgcag gaaataccgg aagcggagta atcgaaattt
    12721 tgcgtaacgg ttttcgctgt gatgttctag cttatgatcc ttacaaatca gatttataca
    12781 tcgaaagctt tggtgcaaaa aaagtagaac ttcaagaatt actggaacaa tcagatatca
    12841 tttgtttatg tgcgaatttg acagaagaga actatcacat gatttctgat aaagaagtcg
    12901 atcagatgaa agaaaatgtt tatctatcta atagtgcaag aggcgctcta gttgatgaag
    12961 aagcgatgat taatggtctg aaaagcggaa agatcgcagg atatgccaca gatgttttag
    13021 aagaagaacc gggacgtcaa gatcatccgt atttagcgtt tgaacaagta gtgattacgc
    13081 cacatatatc ggcctacaca agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag
    13141 atgtcgaaca gatcagtcag caccttcttc ctgaacgttc tgtccagaca aagagcaact
    13201 atataaaagg atgatgaaga aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt
    13261 ttatttatat gaagaaggtg gtatttcaaa agtttctgat ctcttgaaat catacggcgc
    13321 gaaaagagta ctggtcaccc acggaacggt atcatgggaa aaggcgctac ctaaattagt
    13381 atttttgaat gacgaaacga ttcaattttt ttatcatcgt tattctggag aatgtagtta
    13441 tgctgaagcc agacgaatag ctacaatcat caaaaaaatg aaatcgattt cgtagttggc
    13501 gttggcggag gaaaactgac cgatctagtg ggttatagtg ccaatcttgc aagaatcgat
    13561 ttcggagtca tccctacttt agctagtaat tgtgctccat ggacacctct tgctgtcatg
    13621 tataaggaga atggacaagc agaagggaaa acagaacact tttacagaca agctgctttt
    13681 tttataacag atccccaatt agtgattgat tcccctgttc catatttcat tgccgggtta
    13741 gcagatacac tagcaaaatg gtatgaatct gagctcatct tagaacaaga ttttttgcaa
    13801 gatgaatcat ttttgaagct agcacaagat actgcgaaga tctgcaaaga agagattttg
    13861 aatcattctg ctaaagcaat ccaagatatg gcacaaagaa aactgacatc ggagttcaaa
    13921 cagctttcag agattatctt tgctgttgct ggtttagttg gaggattagg agataaatac
    13981 gcaagaaatg tggcagcaca tgctatgcat gacgctatca gtaaatatat tccggaaagc
    14041 catcgttttc tacatggaga aaaagtagcg tatgggattt tctatcaact ggcactagaa
    14101 ggtcgctggg aaaaaataga cgagttatat cctttgtatc gggaattaga actgccggtt
    14161 tctctagaac aaatgggatt atttccgaaa gataaacaga taatagataa aatggctgaa
    14221 tttattgatt cgaaagagaa agtgcatttg attcctatag aggtaacaca gaaatcactg
    14281 atacagtcaa tgatggaact ggaaggttat atcagaaaga tctgaaggag gaggacacat
    14341 ggaaacatta agtgtagtac aaagtttgtt gattgcttta tgggtagccg cggttatgtc
    14401 ccgctggtta ggtggaggag ccacgttgac cttacgtttt tctcctttga tgactggatt
    14461 agtcgtcggg ttgatcatgg gggaagttgc acaagcgatg attgttacag ccgctttgca
    14521 aatgatttat atgggggttt tttctccagg aggatcaatg ccagctgaac catctattgc
    14581 agcagctatc gcagtaccag tcgcattatt aggggatctg tcgccagaag cagcgattgc
    14641 cgttgcggtg ccagtcggat tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa
    14701 tactttttta ggtaaatata cagacaaagc tgtagaagat ttgaatacaa aagctatggc
    14761 ccgatcaatt attctttatc caacggttgc ctcattttta ttattcgttc cgcttgtttt
    14821 cattgcttta tatttagggg cgccattgat cgctgatgta atcaaagctt tagagggaac
    14881 agtcgttatc cacattcttg aagtagtagg tggagggtta gcggcaatcg gtatcgcgac
    14941 aactgtatac gttatcggtc gaaaagatta tctggtcttt ttcttccttg cttattttat
    15001 gagtgttgtt ttgaaatctt tagatattac gatggttact tatgccattt ttgggattat
    15061 tatcgctttg atctttgtcc agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa
    15121 taacaattca gcatcttttg atgacgaaga tgactatgat gacggatttt aatttaggag
    15181 gaatgggaaa tggctgaaat aatggataat caaacaggtg ctcctgaaga aatcacgaaa
    15241 aaagatgtaa caaaagccta tctgagatgg cattttgcaa acgaaattcc tcactctttt
    15301 gaacgttact tggcgccatc actactatac ggaatgatgc caatcttgaa aaaactttat
    15361 aaagatgaag actctttaaa agcagcatat aaacggcagt tactgttctt caacactcaa
    15421 ttaagctggg gtggcggtgt gattactggc ctaatgtctt ctatggaaca gcaaagagca
    15481 gaagaagagt atgaagaaaa agagatcttg atgcaggatg acttgatgta taacacgaaa
    15541 gcgggattga tgggggcact agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa
    15601 tatattttta tcgcaatcgc ggtaccttgg gcacaacagg gaagtccttt aggcgcactt
    15661 tttcctttta tctgctttgg gttgtatcag gttttactag gggtattttt cgcaagacaa
    15721 gctttcacta tgggccgtaa tgctacaggc ctaaagcaaa acactggtgt tcagacagcg
    15781 attgaaatgc tatcagtttt agggttgttt atgatggggg tgcttgcggg aaactatgta
    15841 aaagtagaat cttcattgaa atttgcgata tccggacgtg agtttgttat ccaggaaatt
    15901 cttgatcaaa tcgtgccagg gatattacct ttagctgtcg taatgggtgt gtacttgttc
    15961 tatacgaaaa aaggattgaa agtcacacaa gcattattat ggctgactgg aatattaatt
    16021 gtgctagcag gaatcggtat tttataaaaa gaagggaacg tgaataagat gggtgtagtc
    16081 aatttagcgc gtgtggatga gcgtttgatc catgggcaag taatggtaac gttatctcaa
    16141 aaaagtggag tgaattccat ttttgttgta gacgaagttg tagcaaaaga taaatttatg
    16201 agagatcttt acaaaaatgc aggaagccgt acaggacaaa aaacaattgt cattacacca
    16261 gagaaagcta aattctattg ggatgaatat caatttaaag aatacaattg tattttgatc
    16321 gcaaaaacag tccaaacgat ttacgactta gtaaaacacg gaatccccat ggaagaattg
    16381 aatattggag ggattgctca aaaaaatcct gatacagatc ttttagtgac gaaatctgtt
    16441 tatttaaata aatcagatgc tgaaaaattg aaagaaatga atgaacaata tggcgtaaag
    16501 aatatttatt ttcaagccac tccatctgca cctaaaacag gattaaaaga tgtattgaac
    16561 aagtttgatt tgtcataaac aaatgcttag aaaaaagata aagagggcga aaaaatgaag
    16621 aagaacgagc tgtttcgtga ttgggaattt cgttatcgct atatttaccg taagcgccgt
    16681 acaaaaaaga gtaaacagcg ttttctttca gcattagtat cagatattta cagtatgaga
    16741 acagatgtga cagtcattgc ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt
    16801 ggagatatcg aaaaagcaga gaaagttatc tgtacttatt acgatacacc cgttcatgca
    16861 ttaggaagtt atttcatgtt tgattggaaa gaccaaagga aaaaaacaat ctactctata
    16921 ttattgtcct tcattttgtt gttcagttta ggttggtggg gaatgatgat atataacaaa
    16981 aatcctcatc acgtattcga tctgctatcc gtataaacag gtatcactgt actagcattt
    17041 gggagctact ttttcttatt gggaaaagca gctcgcggat ggcctagcag acaaactttt
    17101 attcgaaata cttcatccat attaacgatg ctagaaatga tccgtacgat tgatgatccg
    17161 agtgtagcgt atgcgtttgt tgatgaaggc tgttatggag aaaagggctt agacagcgtg
    17221 agatcgggta tgaaaaaaga ggctattctt ttttatctag actctgttgg ggcagataca
    17281 ccgctacagt tttcgggaaa ttatttttca aataaagaac agtggctgaa acaagttgat
    17341 aagttgaaag aaaaaaacgt caattatatt tttagtgcaa gaaaaaagca agcgcagttc
    17401 ttctatttga ccaaaacaga tttacgaggg aaaacattta attggcaaaa tgccaatcag
    17461 attattgctc tttttcgata agtaaaaagt gcaaaacaag gagtgagaag actaatgttg
    17521 gaaatcatga tcgttacaca tggtaagttg agcgaaggac tgaaagatgc ggcggaagta
    17581 attgtcggga catcgagcca tattgaaaca atgtgcttag agtctgggga ggacatccaa
    17641 acattcggag agaaggtcaa agaaaaatta actatgatca acacagcaga acccatgcta
    17701 gtatttgttg atctaattag cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa
    17761 ctgactgaag aaaagaaagc agccacttat gtcatagggg gcgtgaatct gccaatgttg
    17821 ttagaagcaa tcaatcatca gatgcttcac acaccgatag aagaagcggt ggaacaaatt
    17881 gtcaaacaag gaaaagataa tttaactgtt tggcatgctt cagcggaaga aaaaaaatta
    17941 gatgaggacg atttttagaa ggaaggctga aaagatgaag tggggattta gatggtatgg
    18001 tgaaaaaggg gattcgatcc cattaaaata tgttcgccaa ataccgggaa taaatgggat
    18061 aattggaact ttgctgacta aaatgcctgg agatgtttgg gagatttcag aaattgagga
    18121 gttgaaagca tctgttgaga gccaaggact ggaactgctt ggaattgaaa gtgtagcgat
    18181 ccacgatgca atcaaggcag ggacagatga acgagattac tatattgata actatatacg
    18241 aacaatcaaa aacttgtctc gatgtggggt tcgattgatt tgttatagct tcaaaccaat
    18301 ttttggatgg gcgaaaactg atttgtttaa tgaaaacaac gatggaagct attctctttt
    18361 atacgatcag aaaatcgtcg atcagatgga gccaggagaa atgtatcaat tgatacacag
    18421 ccagtcaaag ggattcaaat tgccgggttg ggaagaagaa cggttgaaaa aattcgctca
    18481 gctggaaaaa ctgtatgaag gaatgaccga agaagaatta tttgaaaatc tgacttattt
    18541 tttagaaaaa gtgattccag tgtgcgaaaa agcagatgta aaaatggcta tccatccaga
    18601 tgatccgcca tgggaacttt ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa
    18661 gaaaattcta aatgcagtag attctccata taatggacta acgctgtgca caggatcttt
    18721 aggggcaaat ccggaaaacg atttagttga aatcatccat gaggtaggtt cgcgcatcca
    18781 ttttgtccac ttcagaaacg tggagttttt aggagaaaga aagttcaaag aaacggcaca
    18841 tccctctaca gaaggctccc ttgatatgta tcatattatg aaagcgttgg tgcaagaagg
    18901 gttcgacggt cctatacgcc ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc
    18961 tggatatggc ctgtatgatc gtgcaatggg actcacttac atgcaaggtc tatatgaggc
    19021 gataagtaag gaaaaaccac tgggagagca ttatgaataa aatcaagctc ttcatctttg
    19081 atatggatgg ccttttatta gaaactggac gtttagctta tcgtgcttat gtaaaatcag
    19141 cacaaaagta tgattatgaa atgcgtaagg aagtctatta tttactaact ggccaaacag
    19201 aaatggcgat aagagaacaa atgggcattt tgtacggaga agatgttcca tatatcaaat
    19261 ggcgagaagc aatcaatcaa tataaagaaa aaatcgtgaa agaagataag agagtatata
    19321 cgaaaaaagg agcagaagaa attttatctt ttgcaaaaga aagaggaatc catacaatcg
    19381 tggcttcgtc taatacacgg gtaaaagtag aaatgtattt aaaaatggaa aatctatatg
    19441 ccttgtttga tcaaatcatt tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga
    19501 tctttttgaa agcttgttca aaaatgaata ttcctccaag tgaagcactt gtatttgaag
    19561 actcgattgc agggatcgaa gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag
    19621 atcatttgat agggttgccc gatcataatg ggaagcataa gttgaaagta gatgtaagtt
    19681 tatatgaatc caataaagca gactatgaat tttatgacct gcatcaagca aaacgatttt
    19741 tagaaagtaa agggttgtat ctctaataaa gaggactatt ttctagtcct ctttatttat
    19801 tgtcctgcac gactgaggga acagatgaaa aagaagtaat tttttatttt gaagtatcta
    19861 ccttatttgt agtgaacaat ttagaacaaa cacaaaacgc atctactgtt atctaccagt
    19921 agatgcgttt aaagagaaga acgagaatcg tccatatgag ggtttccact ccttatgcag
    19981 atttttttaa ttcttgcact gcttgttcga tcgtaacacc tgtcgctaca tgattggtat
    20041 tttctttgtc aaaaacgaca aatagattca aatctgtatc caaggttact cgatattgta
    20101 tatctttgtt atagaatgtc atagccattc tcctttcttt aaatacttgc tgaaaggcaa
    20161 cgaacgcgtt gataacgaaa ttccatttat aagtatacca ccaattgaaa ggttttcata
    20221 gttaagaaat tttaaagtca aaagttgcag ataaaagcaa ataaacaaga aaacaaaaag
    20281 tgaatcaatc gcggttattc aactttttct tcgattcgaa gacaatagaa gacaatgaaa
    20341 tttatatttc attgcattat ccttgtgtta ttagaaaaag catgatatat taaatgagta
    20401 atctattttg aaacgtaatt tgagcgatat atctacatag ataaaactcc ttttgcctat
    20461 caaatattta ttgcaacgaa aaaacgtaca ttgatacgta tcaggaggaa tttatttatg
    20521 aataacggta cagtaaaatg gttcaacgca gacaaaggtt ttggctttat cactggtgaa
    20581 gatggaaatg atgtatttgc acatttttca gctatccaag gtgacggctt caaaacttta
    20641 gaagaaggcc aagcagtgtc ttacgatgtt gaagatggtc aacgtggccc tcaagcaaca
    20701 aacattgtta aagcttaata aaaattgact tttaaccgtc ttttcgaaag aaaa
//
LOCUS       DAZKOM010000044        20706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000044 DAZKOM010000000
VERSION     DAZKOM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.044"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8NF_001557"
     CDS             352..1041
                     /locus_tag="ACG8NF_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0472045.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8NF_001558"
     CDS             1028..2236
                     /locus_tag="ACG8NF_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0472046.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8NF_001559"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8NF_001559"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0472047.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8NF_001560"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8NF_001560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0472048.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8NF_001561"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8NF_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0472049.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8NF_001562"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8NF_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0472050.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8NF_001563"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8NF_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0472051.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8NF_001564"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8NF_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0472052.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8NF_001565"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8NF_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0472053.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8NF_001566"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8NF_001566"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0472054.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8NF_001567"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8NF_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0472055.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8NF_001568"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8NF_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0472056.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8NF_001569"
     CDS             14660..15043
                     /locus_tag="ACG8NF_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472057.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8NF_001570"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8NF_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0472058.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8NF_001571"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8NF_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0472059.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8NF_001572"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8NF_001572"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0472060.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8NF_001573"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8NF_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0472061.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8NF_001574"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8NF_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0472062.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8NF_001575"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8NF_001575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0472063.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5929 a         4220 c         3801 g         6756 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaa
//
LOCUS       DAZKOM010000045        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000045 DAZKOM010000000
VERSION     DAZKOM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.045"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8NF_001576"
     CDS             291..1430
                     /locus_tag="ACG8NF_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0472064.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8NF_001577"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8NF_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0472065.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8NF_001578"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8NF_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0472066.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8NF_001579"
     CDS             3334..4302
                     /locus_tag="ACG8NF_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0472067.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8NF_001580"
     CDS             4415..4693
                     /locus_tag="ACG8NF_001580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472068.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8NF_001581"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8NF_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0472069.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8NF_001582"
     CDS             5150..5905
                     /locus_tag="ACG8NF_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0472070.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8NF_001583"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8NF_001583"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0472071.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8NF_001584"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8NF_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0472072.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8NF_001585"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8NF_001585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0472073.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8NF_001586"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8NF_001586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0472074.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8NF_001587"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8NF_001587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0472075.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8NF_001588"
     CDS             10642..11184
                     /locus_tag="ACG8NF_001588"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0472076.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8NF_001589"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8NF_001589"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0472077.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8NF_001590"
     CDS             12777..13679
                     /locus_tag="ACG8NF_001590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0472078.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8NF_001591"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8NF_001591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0472079.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8NF_001592"
     CDS             15122..15544
                     /locus_tag="ACG8NF_001592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0472080.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8NF_001593"
     CDS             16013..16243
                     /locus_tag="ACG8NF_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0472081.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8NF_001594"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8NF_001594"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0472082.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8NF_001595"
     CDS             17654..17830
                     /locus_tag="ACG8NF_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0472083.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8NF_001596"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8NF_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0472084.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8NF_001597"
     CDS             18325..18708
                     /locus_tag="ACG8NF_001597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472085.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8NF_001598"
     CDS             18877..19761
                     /locus_tag="ACG8NF_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0472086.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8NF_001599"
     CDS             19875..20111
                     /locus_tag="ACG8NF_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0472087.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG8NF_001600"
     tRNA            20199..20279
                     /locus_tag="ACG8NF_001600"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG8NF_001601"
     tRNA            20286..20357
                     /locus_tag="ACG8NF_001601"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKOM010000046        20045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000046 DAZKOM010000000
VERSION     DAZKOM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.046"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..413
                     /locus_tag="ACG8NF_001602"
     CDS             117..413
                     /locus_tag="ACG8NF_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0472088.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1057..2010)
                     /locus_tag="ACG8NF_001603"
     CDS             complement(1057..2010)
                     /locus_tag="ACG8NF_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0472089.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2033..2788)
                     /locus_tag="ACG8NF_001604"
     CDS             complement(2033..2788)
                     /locus_tag="ACG8NF_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472090.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2785..3744)
                     /locus_tag="ACG8NF_001605"
     CDS             complement(2785..3744)
                     /locus_tag="ACG8NF_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0472091.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3737..4687)
                     /locus_tag="ACG8NF_001606"
     CDS             complement(3737..4687)
                     /locus_tag="ACG8NF_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0472092.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4837..5289
                     /locus_tag="ACG8NF_001607"
     CDS             4837..5289
                     /locus_tag="ACG8NF_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0472093.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5378..7645)
                     /gene="gshAB"
                     /locus_tag="ACG8NF_001608"
     CDS             complement(5378..7645)
                     /gene="gshAB"
                     /locus_tag="ACG8NF_001608"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0472094.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7832..8830)
                     /gene="ftsY"
                     /locus_tag="ACG8NF_001609"
     CDS             complement(7832..8830)
                     /gene="ftsY"
                     /locus_tag="ACG8NF_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0472095.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8841..9650)
                     /locus_tag="ACG8NF_001610"
     CDS             complement(8841..9650)
                     /locus_tag="ACG8NF_001610"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0472096.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9659..13240)
                     /gene="smc"
                     /locus_tag="ACG8NF_001611"
     CDS             complement(9659..13240)
                     /gene="smc"
                     /locus_tag="ACG8NF_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0472097.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13260..13946)
                     /gene="rnc"
                     /locus_tag="ACG8NF_001612"
     CDS             complement(13260..13946)
                     /gene="rnc"
                     /locus_tag="ACG8NF_001612"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0472098.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14294..16075)
                     /locus_tag="ACG8NF_001613"
     CDS             complement(14294..16075)
                     /locus_tag="ACG8NF_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0472099.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16101..17015)
                     /locus_tag="ACG8NF_001614"
     CDS             complement(16101..17015)
                     /locus_tag="ACG8NF_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0472100.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17042..18004)
                     /gene="opp4B"
                     /locus_tag="ACG8NF_001615"
     CDS             complement(17042..18004)
                     /gene="opp4B"
                     /locus_tag="ACG8NF_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0472101.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(18004..18948)
                     /locus_tag="ACG8NF_001616"
     CDS             complement(18004..18948)
                     /locus_tag="ACG8NF_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472102.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18949..19953)
                     /locus_tag="ACG8NF_001617"
     CDS             complement(18949..19953)
                     /locus_tag="ACG8NF_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472103.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5588 a         4293 c         3557 g         6607 t
ORIGIN      
        1 aaaataaaaa acctttaaca actattggaa aaagaattac tttttgttca cggattatta
       61 acaactcttc ggtagagtaa acttatcaaa taaaagactt ctttggagga atcactatga
      121 aggaatatat aaaaaacatc tattttatcg aagaaacaca aaatatcgag ggttcttata
      181 ttgaagtaaa aacactcttt gtcaatgaag ataaaaccaa agcattggat atttataaaa
      241 aattagcttc aaaaaaaacg aatagttttg gattgatatt aagcgagtat aaaatcaaag
      301 cagaagagag ttatttctat caattactaa aacggtggag taagcttccc gccgattttt
      361 atcgaaaaat gcagatcatc aactatcaac cgcttgctga gacacatgcc taaatagagg
      421 ttgtgaaatc agtgctttga gctaataccg aaggtcagct gattgaacat tgtttagaag
      481 agattgtgac aactgcgatt tgctttgagt aataagaacg ctggacagaa aatagcttct
      541 catattttct agtctagtgg gcttattgcc gagaagcagc agatggaaca tcgtttagaa
      601 gagattgtga caactgcgat ttgttttgag taataagaac gctggacaga aaatagcttc
      661 tcatattttc tagtctagtg gacttattgc cgagaagcag cagatggaac atcgtttaaa
      721 gaagagatcg gtacaaaata aaaatatcta aatttaagtt ttatctaagt ttcgctttgt
      781 acctttgatt caaatcaaag gaggcgatct tgatgctgaa aaaatggatc acactatttt
      841 ttgccactcc ccattctaga atcaacccgt atgatgtaga taatgaattc acgttgaaaa
      901 aagatttctc aaataaataa aaaccatccg taaaatcatc aatacgattt tacggatggt
      961 ttttgataac ttctttgagg ataggctctt gctatttaag aatgtaagaa acattattgt
     1021 tacatagtat gagagtttaa tcctctgtta tttatgctat ttaaatgctt gattcacatc
     1081 ttcgatcatc agtttcattg attctaaacc gcccccgctt aaataccaga catctggttc
     1141 aagggagatg atctgctgat ttttaccagc attcgtttgt gctaccagtt cattggcaga
     1201 aatatcatct ttgctgtcgt ctccaccaat agcttttgtt ctgtctacta caaataagat
     1261 atccggattt ttttccagca catattcata agaaacactt tgaccgtgag tggatgcttc
     1321 gatttgatca tctgcttgtt caaagccaaa taaatcatgg atgaaaccga atctagaacc
     1381 agatccgtag gctgataatt gcccttcatt cacaagtgtg actagtgctt ttttgtcagt
     1441 agcttctgct ttctttttcg tctgatcgat tgcactagat aattcctcta attgagtttt
     1501 agcttcttct tgcttaccaa agatctcccc taaagcagtg acattttgct ggaaagattc
     1561 ccatggattt ttcgcatcta aagaaaggaa aatggtcgga gcaatttgac ttagatcttt
     1621 ttgaaaatct ctttgtcggc cagaaatgat gattagatct ggctgaagct gattaatttt
     1681 ttctaagtct ggttctttta ttccacctgc tgattctact tttttatagg atgaaagata
     1741 ctcaggaagg ctgcttgtag ctgctccaac taccttatct ccaacaccaa gagcatccat
     1801 cgtatctaaa gaaccattgt cgaatacgac gactttttct ggattatttg gcacctcgat
     1861 ttggtctcca ttactatctg ttatcgtcaa tgtagtttca gtttgtgtgc tgcttttttt
     1921 tgtgtctgcc tgactggtgt tgttgctatt tccacatgct gctaaaccaa atgatgctag
     1981 aatcaagata cctactacta attttttcat ctcttctctc ctaatgttag ttttagaaat
     2041 aaagacagaa tctgcggcct tcaatctcac aaatccgtat gtccatttcg tataattcat
     2101 ccaatacatc tttttttatc atttcttggg tcgttccttg cgtaaataac tgtccatctt
     2161 tcattgcaac aatttcgtct gcataacttg cagcaaagtt gatatcatga agaacaataa
     2221 caattgtttt cccgatttct tgtactaaat tttttaaaat ctgcatgatt tgaaccgcaa
     2281 aattcatatc tagattgttc aaaggttcat ccagtaaaat ataatccgtg tcttgcgcta
     2341 gcaccattgc aatatacgca cgttggagtt gaccgcctga aagagtgtca atcgattgat
     2401 ccgctatttc ttctaaccca atgtgttcca gtgattcctg aacgatttcc tcgtcttttg
     2461 tcgtcaattt tcctttcgta taaggaaatc gtccaaattc tactaattct ttcacggtga
     2521 tttggacatt tatttcattc gattgtcgta ggatcgataa ttttttggct aattcattgg
     2581 atttccaagc ctttacttcg ttatggtcta aatagatttc acctgtatct tttggtatca
     2641 aacgactgat catcgacaac accgttgact ttccagcacc atttggaccg ataaaagcag
     2701 tcaacttctt ttcaggaatc gttaactgta catctttaac cactgatttt ccatggtaca
     2761 gtttattgac cttttgtaaa tcaatcatag gatactcttc ctttcgcgaa tcaattttgt
     2821 gacaaaataa attcctccga caaattcaac gacgacactc actgttgtat tccagccgaa
     2881 aatctgctct actgcgagct gtcctcctgt cagaaacagc atagctacca gaaagcttcc
     2941 gataaatagc tgaccatgac gataagtaga aaagacacga taactgatat ttgcgacaat
     3001 aaaccctaaa aaagtaactg gacccacaag agccgttgat gtccctgtca aaagactgac
     3061 acaacataaa atcaaaaatt caaaacgatc tgtgtctatc ccgaggttta ctgcttgttc
     3121 atttccgaga tggaaaacgt ctagcacatg tcgatatcga aataacaggt accagcagcc
     3181 gataatgatc aaagaagcag gcaaaagata ctgactctgg atattgccaa agcttgcaaa
     3241 taatctgcct tgaagcaaat catattcatt cggatccatg atgacttgta aaaaggtact
     3301 gacactacca aaaagcgtac ctaaaatcat ccctgacatc agtaataaaa aaagattcgc
     3361 tctcattttt ttcaaaagaa agaaaaataa caatagactc agcccgacca tcagcccaac
     3421 gttcaataag aaaaaaactg tacctgtctg ctgaagcatc gtcactcctc cgataaagaa
     3481 gaaaaggacc gtttggacaa ggacatataa cgactcaagt cccaatactg aaggagtcaa
     3541 aaactggtta tgcgtaattg tctgaaagct gattgtggca ctgctgacag ctagcgagac
     3601 aaggataaat gcaaaaattt tcttactcct caatgcacat gcaaaatgcc agtttccata
     3661 tgtatggatc gtcaagtaca ttcctgtaac aaataaaaca agaacaagca taccaataaa
     3721 aatagatggc ttttttttaa gcatatcgtc gtccccttat cagaagataa aggaagatag
     3781 ccccaccgat gattccaacg atcacactga ctggtatttc ataaggaaaa atcaacacgc
     3841 gagcgacaag atcgcaaaat actaagaaca aactacctgc taaagcagtt acgccaattg
     3901 ttttgtgcac ttgatctcca tatattctag agacaagatt cggaatgatc actccgataa
     3961 aaggaatgct tcctaccatg atcaacatga ctgcactagc caatgatact aagccaatgc
     4021 ctaaaaactg catcttctgg taatctattc ccagatttac tgcaaacgat tcgcccagac
     4081 caatgatcgt aaatcggtaa gcaaatagcc aaaccaagag caacaatgga acaacagcat
     4141 acaataactc ataatttcct cgtatcacag tcgcaaaatt cccttgaagc catgaagtca
     4201 tattttgtac caactggaat tgataagcaa aaaaagtagc aactgaacca atgatattcc
     4261 ctaacatgac tccaatcaac ggaagcatca attgattttt ctggaggaac aatcgcgtaa
     4321 gggataaaaa aagcaatgtt cctgcaaaag caaagcaaaa tgctgtcaag gatcggacaa
     4381 tcaaggatgc accaggtagg aaaatcatag ccactaaaat acctaaacgc gcactatcca
     4441 tcgtgcccgc agtcgttggt gaaacaaatt tattttgggt caaatgttgc atgatcaacc
     4501 cacaaatact actggtcgct cccacaagga tcaaacaaat cgttcgtggc aaacgagtca
     4561 ctagcaagat ttgtaagctt tgcccatcta actggagcag ttctgacaat tgtacattcg
     4621 atactccgat gaacaatgaa atactactta aaataactaa taatgctact agtcgtccgt
     4681 agcgcatcca atccaccttt ctaattgaga atgattttca ttcttatttt aagtgaatcg
     4741 ttgaacaaaa gcaaccgctt tttcatatgt tttaggtatt attgggaata tgctataatc
     4801 gtaagaaaga caaagagaag aaggagggta ttttttatgg cacaagatga tgtaaaaagc
     4861 catttggata agggacttta tggtacacct ttagtgaacc ctgaagaaca gcataaatat
     4921 ttagggacat tcagggaacg ctgctatctt agtatgactg ttgctgaaat gatccaacca
     4981 aaaaacaaag aaaacttttt aaaagaactc aaaaaacatc cagatgcttc catactcttg
     5041 aatggggctc tacccctttc tgtccaaaac caatatatcc agctagcaac gaaaaacaat
     5101 agccgtttca ctgtagtcaa cgattacgtc actgcaaatc ctgaggaatt cggattgctc
     5161 ttaacagcaa aagaagcagt aaatgaacct gtgattgata tcgaaaaaaa atacccagaa
     5221 gaaaactctt cgccagctga aaaagaaaaa ccaaaaaaga aaaaaagctt ctgggatcgt
     5281 ttattttaaa acgaaaaaac acataaattt ttacatgcaa aaaaagcttt tgatcagatt
     5341 gacatcaaat aggacaatct gtcaaaagct ttttctttta aaatacttca ggatataaca
     5401 atttcaatac attcatcgtc aatcttctgc cttttcctgc aaaaggataa acatgcatat
     5461 gcatggctgg attgaaattt gcttcgatga tcccatatgc ttttttatca gttgtaggat
     5521 cgatttcttt atctggtata atcaaatcga taccactaat cttagctcct agtgcttcta
     5581 ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc atcagtcata tcgatcgaat
     5641 caccacctgt actgatattt gagttctccc ttagataaac aatttgattg gcttggggaa
     5701 ctgattcgat cgtcaaccct tgttctttca acatcaattg ttctaattca cctaattgga
     5761 tcaattctaa aggtgcacga tgattcgttc cacgcaaagg gtcactattt ttttcttcaa
     5821 caagttcttt cactgttcgg atactgtcac caataacatt agcagggact cgcaacatga
     5881 tggcttggac ttctccgtcg atcacaaaga aacgatattc cgttcccggc ataaattctt
     5941 ctactaaaac cgaactgtct tcacgaaaag caatggctaa acctgcttga taatcttcca
     6001 gactagctcc ttccttgaaa atcgtgatac ctaacccgta attggttgat ttaggtttga
     6061 tcacaaatgc ctgctcggca aatctcggat aagcttttac cgcttcttcc atggatgaaa
     6121 attctgcgcc tcctggtacc cggaacccag cttcttttaa gacttttttc gttactgttt
     6181 tgttttccat gataagtggg acgatatagc tgtccttact tgtcatgttt gcatttttca
     6241 catattctac atggtcttgg aattgcagac gcaaaaattg atccgactca tctaatactt
     6301 tgacttggac acctttttgg atggcatcga acataaatat ctgagtagaa agttccattt
     6361 cgcgaaaacc ggccaactga tagggacgtt catggctttc tttataatat tgatgtccca
     6421 atgccacagc aacttcttgc tgactcgata gctgtgcttc ttgatagagt ttcgctgcta
     6481 acgtttgttc aggatgatcc atttgagtca atgcttcctc tactaaataa agagaatcca
     6541 accaatccaa ttcaaccagc atttgcttca ttccttcaag aatttctcta ccttcttgat
     6601 gaaattctgt ttgatctgat ggttgttcta gtgctacttt gttatttcga gtcgtacctt
     6661 ctgctaccca ttggtcacag tcttcttttt cttctgtcca taacatgtac atcaggaata
     6721 attgtaagaa ccgaacttgc tctggattga tacccaatct tgcataagga ttcaaatcga
     6781 tattccgcaa ttcaatataa cgaacacctg ttgttgctaa atctgcgact ttctttccgc
     6841 ctcggaaacg aagcggtgta taaaattctt tctcctccga aagtttccct tcttcgatca
     6901 ttcgctcgat atctgctaaa tactgtttca tagaagcata ggaaactttc acatttggat
     6961 gattcgtata gcccagcgca ctgttgcgaa tactgcgaac aggttcttgc ggatgtgatt
     7021 catctaaaaa gtagttcttc tcactcattg gtgaagcacc gaataaataa gtgatcatcc
     7081 agcgatagcg cataaagttt ctcgctgttt tcaaataaag ttctgttttg aattcggaaa
     7141 aatcttgaaa ttcttcctga tggctgaaca atgttcttag caactcatcg ccaaattcaa
     7201 aattgaaatg aataccgctt accatctgtt ttcgcttccc gtattctttt gctaaataac
     7261 gtcgatacaa gacatcttcg aaatttttta attttgcaat aatgatctct tcgtcttttt
     7321 ccggtaaggc aggtggcata cttaatggcc agatcatctc ttctttcggt atagaacgag
     7381 cagtcacatc ataaacagca gcgagatact gaaataattc gggaatagaa tcggtaacag
     7441 gggtgatcat ctctgtttgt gtttcgctaa aatctgtttg gatatagggg tgataggatc
     7501 gatcgccaaa gattgctgga tgatcggttt ttgctagatt tcctgcaaga tcaacccgct
     7561 gtccttcccg ttctatacca aatcgagctt ggtcgataaa agggcgcgca ttgacatgca
     7621 ataataattg cttaaaattc atcattaagt gacctccaat tcatatgaat ctaattataa
     7681 tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc gcttacaaat aagtataaaa
     7741 atgaaggcat caaaaacaca gctatgttaa aaacaatcat tcaaacaagg agctgcaact
     7801 tttgtcacag ctcctgcttt ttagacatgg gttattcttc ttttagtaat cctttgaaca
     7861 atccaacgac aaaatcattt ggatcaaacg gctccaagtc atcgattcct tcaccaagac
     7921 cgaccaattt cactggtaaa tgcaattcat tacggatagc aagtacgata ccgccctttg
     7981 ctgtaccatc taattttgtc aaaacaagac cggtcacatc tgttgtttct ttgaattgtt
     8041 ttgcttgaac catcgcgttt tgaccagtgg ttgcatctaa tacaagaagt gtttcttggg
     8101 gtgcttctgg taattcccgc tggattactc gttttatttt ttctaattcg ttcatcaagt
     8161 tcactttatt ttgcagtcgg cctgcagtat caactagtaa aacatctgcc tgctctgtct
     8221 ttgctctttc caaagcatcg aatacgacag ctgctggatc gcctcctgca ttgccacgaa
     8281 caacttcaac accagcgcgt tcgccccaga caactaactg gtcgattgct ccagcgcgga
     8341 aggtatctgc tgctgccatc aatacttttt taccttcgtt tttgaactga tgggcaagtt
     8401 taccaatact tgttgttttc cctacaccat taacaccgac aaacaaaata actgttagtc
     8461 cgtcttcttg gatatttagt tcatttactt cattgattcc ttccgcttca tagagatcca
     8521 caagtttctc gatgatcgca ttttggactt ctgccgattt tttcgcattt ctgagtttca
     8581 cttcttgacg taatgcttcg gtgattcgta tcgctgtttc aaaacctaca tctgcaccga
     8641 taagtgtttc ttctaattct tcaaaaaagt cttcatcgac tgttcgaaaa ttggcaaaca
     8701 actcattcat ccgttcacca aatgttttac gcgtcttttc taaacccttg tcgtacttct
     8761 cctgtacttc aacttcttct gttttctctt cgccagtaaa agcacgcttg attttatcaa
     8821 aaaatcccat ttgactcccc ctactctaat acaaattttt ggatcacttt ggctacgcca
     8881 ttttcttcat tgctggctgt cacgaaatct gctgcttctt tgacgaaagg cacagcattc
     8941 tccatcgcta cgcctagtcc agcatattgg atcatcggta aatcattttc ctcatcaccg
     9001 atcgccatga tttcttcttg tttcaaacca agatcttttg ccaataaaga aataccataa
     9061 gcttttgtaa caccttttgg cataaattcc aaaagcatac ttcttgtttt gatgatctca
     9121 tacatttcat ggaattctgc tggtatttct ttgattttag gatctaattc ttcttggtca
     9181 taagcgatta ctgccttatt aagcacaaat tcatctgtaa tatcgcttat atccgctggt
     9241 tggaattgca gcagcttatt caacacattg tacaaggatt gcttccttgg ggcactaggc
     9301 agctgtaaaa ccacattgtc agacaaaaca tctagtggca gatccaattg ctgagccagt
     9361 ttgtataagc gttggatatc tgatacttcc atcactttct tttccatgat ttcaccagta
     9421 tcattttttt gtaccaaacc gccgttgaaa gtgatgctgt agtcaccttc atctaccagt
     9481 cctaattctt gcagataatg cgccatcgca gctaaggggc gtcctgtaca tagaacgatc
     9541 ttgacccctt gctgttttgc ttgtgctaat gcttgtttat tctcatctga aatcagcttt
     9601 tgttcattta ataatgtacc gtccaaatcg attgctacta atttgatcat tttcgttcct
     9661 acactttcgt ttcgttgatt ttcgcctctt cattgatatc ttccatccgt acagaaacga
     9721 tttttgatac accggattcc tgcatcgtca ctccgtacag tacatctgct gcgaccatcg
     9781 ttcctttgcg gtgtgtgact acgataaatt gtgtgtcatc ttggaaatcg ctcaagtaac
     9841 gaccaaaacg tttcacgttg gcttcatcta acgctgcttc tacctcatct aagatacaaa
     9901 acggaacagg acagacacgg ataatggaaa agagcaacgc gatggctgta agtgctcgtt
     9961 ctcctccaga aagcaaactt agactttgca gttttttgcc aggaggttgt acttcaatct
    10021 caatacctgt tttcagcaaa tcagttggtt ccgtcagtac taattcagct ttaccgccgc
    10081 caaacatatt tggaaagaca attttgaatt cttgacggat agcttcaaat acttctttga
    10141 atcttgtccg cacttcatca tccatctcat ccatcgtttc aaacaattgg tttttcgctg
    10201 ctaacagatc atctcgctgt gttgctaaga atgtatgtct ttcactgact tgttcgtatt
    10261 gttcgattga atttaagttt actggtccca gtttttcaat ctgttctttt aaggatgaaa
    10321 cctttgttct actgtcttcg atatcggttg tttcttgata gtcttgggaa gctttctcaa
    10381 atgtcagctg atattctgac tgcagatagg tcaaatgatt atctaaaatc atttcagctc
    10441 ggtctttttg gacttctaat ttggattgtt ccgacagtcg tgctttttgc tcacgattac
    10501 gctctgccaa gactgcttca agcttatcga tctccttttg ccttctatct cgctgctctt
    10561 tggcttttgc caactcggct tttaatgttt ctcgctgagc agataattcg ttaatttgtt
    10621 tttccaaact ttcttctgtg acttcatgat cagaaaagtc tgctgtcaat gtcgccaact
    10681 gcttttctat cgcttcttga cgttcggttg cctctgcttt ttggactcgg gcaccgcgga
    10741 gctggatctg cagatgatta aactgctctt tcaagacagc aagatctgct tgttcttgtg
    10801 ctttttgact ttgtacttgt gctcttctgg cttccatctg gtcgctttct tgacttaacg
    10861 atttgatttc ttcgtcgatt ttttgtcgtt gcgattcaag atctgtttgt ttgtctttca
    10921 attcgttcgt ttgcttttcg taatcttcga taaactgctg cagttcacgt gtctcaaaag
    10981 ttgagatttg cttttctttt tcaaaacgtt caagttcatt tatgatgttt tgtagctgat
    11041 ttgtcgcttc ttgttcttca aaacgtaatt gttcgcctcg tgtacgcaaa acttcttgtt
    11101 cttcggctaa acgagctgtt tcttgttgca gctcctgaac ttttttttct tgtgcttgga
    11161 gttgtttatc tgcttcttca aattcactag tcaactgttt caattcttga ttttgtacaa
    11221 acaaactgcc ttggtttcct cgtttattcg ctccacctgt catggatcct ccggcattca
    11281 tcacatcacc ttctaaggaa accactcgat attgatagcg gatcgtttgc gcgattgcat
    11341 tggcactagt taagtctttg gctaaaagga tcgttcctag caggttttga accacagttt
    11401 ggatttgatc gggataactg acttgttcac tggcaatccc tataaaccct tctacagctg
    11461 ctgcttgtgt caatatatga gccggtaatt gtcttggttt gattgtcgtc agtggaagga
    11521 atgttgctct tcctcctctt tgctgtttca aatacgtgat ggcttgccga gcatcttttt
    11581 cattttcaac gatgacatgc tgggctgccc cacctaaagc tgcttcgata gctaacgtga
    11641 aatctgcagg aacatcgatc agttcagcca ctgctccgac aatacccgaa agctgctgtt
    11701 tatgctgcaa gaccaatcga accccttggt aaaaaccaaa atagttttct tggatttctt
    11761 gaaggctttt cttacgagca cgtagctgct ggacttgatt cattaattga tacattttag
    11821 gctgttcttt tgctaatgct tcttggaact ttttcccgtt ttgttggatc atttctaact
    11881 tctcttttgt ttcagttaaa gatgatttta gctgcgcctg cttttgtgtc aattcttctt
    11941 tttgtaagat caatcggtct actgatgctt ctacttccga ttgcttggct aacgtctgct
    12001 tgctttttgc tgtttcttgt atatattgac gttctagata cttcagttcg tttcctacag
    12061 ctgcttgttc ttgcatcaaa tcaacatatt gatctcttaa ttctgccaat aattctttcg
    12121 ttgatttttg gtatttttct aattcttgct gtgtttttat gagatttgct tcagcttttt
    12181 ggatctctgt ttctttttct gctgctgctt tcatcaagct ttcttgaagc ttttcaaaat
    12241 gtttaacctt tttttgtgct tctgctaatg aagtttgata ttcttgcgag cttttttgtg
    12301 tatgctttgt ccgctcctgc aaaacatctt tttgtccttc tgtctgcttc agtttttccg
    12361 aaagatctaa taatacttgc tgattttttt cgatcagacg atcggcttgc gcattttctt
    12421 tacgctgctt cgccaaaatg gattcttgtt cttggatcga ctcagatagt ttgcctaatt
    12481 ctagattgaa tttcgcaagc tgtgcttgtt tgttgtccca atcttttttt gctgtcttga
    12541 tttcagcaac catcaaagag acatcggttt gagtcagtgt ttcttttagc cgtaaaaact
    12601 cctttgctgc ctcactctgg gcggccaaag gagttaactg ttcttccaat tcgtggatga
    12661 tgtcttggac gcggctgagg ttgtcttctg tttcaaaaag cttctgttct gctttctttt
    12721 tccgctgctt gtatttcaag acaccagctg cttcttcaaa gatcccacga cggtcttccg
    12781 gtttactact gaaaatcgct tctacttttc cttgagaaat gattgagaac gattcttttc
    12841 ctaatccaga atctaagaac aattcttgga tatcttttaa tctgcagctt tgtttattga
    12901 tgaaaaactc gctttcccca gtccgtcgat aacggcgggt tacactgatc tcattgtact
    12961 ccaatggcag ataatgatca ctattgtcta aaatcactgt gacttctgca atattcagct
    13021 gcttacgggt atcggaaccg gcaaagatga tatccggcat ttttccgcct cgcagacttt
    13081 tagcagattg ttctcctaat acccagcgga tagcctctgt aatattgctt tttccgctgc
    13141 catttggtcc aaccactgct gtgacgctat tttcaaaatc aatgactgtc ttgtctgcaa
    13201 atgatttaaa accggctatt tcaattcgtt tcaaatacaa agctttccct cacttacttt
    13261 tacagtgctg ccaatgcatt ttccgccgca gcttgttctg ctaatttttt cgatttccct
    13321 tgtccagtgc caatcaattg atcatctaca taaacttcga tccaaaatac tcgttcatga
    13381 gctggacctt cttcattgat caatcggtat tcgattgata catcacctga tttttgcaag
    13441 acttcttgta gttttgtttt atgatccatc tcatgtgaaa aagcacctgc tttgacttta
    13501 ggaaaaatca ctttttcgat gaatgcatgg gtcgtgtcga atccctggtc aagatataac
    13561 gcgcctaaaa aagcttcgaa taggtcgcaa agcaatgctg gtcgcgtacg tcctcctgaa
    13621 ttctcttctc ccttgcccaa caaaacatat tgatcaaagt gacattcttt ggcaaatttc
    13681 gacaagctgt cttcgcgaac gatcgcagct cgtgtttttg tcaattttcc ttctggcaaa
    13741 tgaggatata aacggaataa atactgagaa accatcagtt ctaaaacggc atctcctaaa
    13801 aattcaagtc gctcattatc cgataattgc agattgcgat gctcattcac ataggatgaa
    13861 tgggtaaaag cttgttcaag caaatttaga tcgtgaaaaa cgatgttata gttttctttt
    13921 agttcttttg tcagctggtt gtccattttc cacattcctt ttatggatat tttttcgata
    13981 cttccttatt atactggttt tttgtcaaat ttgaaatggt tcggtcaaaa ggtaagtaaa
    14041 gctttaataa tcctagtgat tgacgagaag ttgtgaaaaa ttcgtttagt ttcgagtatg
    14101 aagaaaaaat tttgtgaaaa tggctttcca tatttttgca taaatttgac ttaataccga
    14161 gaaactggac tttgaacacc gtttattgga gaagtcatga aatcagcgcc ttgacctgaa
    14221 tattagagaa ataaacagaa aaatatcaaa aaaccgaaaa aggacatcgc cctttttcgg
    14281 tcatttttag tgattatgaa cgtgaatctg ctgtaacaga aacatttgcc catgggtttg
    14341 gtacatcata agaccaatcc cagtctttga cacggtcgtt gactggcaat acttcattac
    14401 ggaacaacgt tggaatcgca aatgcttctt caaatgcata ttgttgccat ttttgatatg
    14461 cttctttttg gtactcttca tcaaatgctt tgcttgaact gatatcgttg atcaattttg
    14521 tattttcttc tgattcataa cgagtgtagt tgaacgcagc atttggtccc catagtccac
    14581 ttggatttgg atcagttcct gtattccaag cagcttggta aacatcaatt tcaggatcat
    14641 cgttttctaa tttatcatag aaagaattga attcgatcag acgacctgtt gtgtattgga
    14701 catccaaacc aatttctttc cattgttgga tatagtaatc tgccaatggt tgcgctgttt
    14761 ccccaccgga catcgaagca aagttgattg ttagtttatt tccatctttg tcttctctta
    14821 aaccatcgtc atccacatct ttgtagccag catcatctaa tagttttttc gctttgtcta
    14881 gatcttgtgt gaatcctttc atctcatcat catggtatga ctggaatact ggaggaatca
    14941 atgaagtcgc attgcttctc aaacctgcat agaaacgttc acctactgca ttattatcta
    15001 atgcatagcc cattgcttga cgtaatgatt tatcagccat ttttgaatct ggattgtatt
    15061 ccacactctt tgtatctgca ttccatttac ctaacttgaa tcctagataa gtataagaag
    15121 tttgttcacg gcctaagttt gtatagccag caatttcttt ataggtatcg taagtatctg
    15181 ttggcatttg gaaaatcata tcatataatt tagagttcaa tgcttcagtt gcacttgcca
    15241 tcggtacaga tttcatcgtg atctttttca atttaggcgt accattccag tagtgctcgt
    15301 ttggcaagta ttctactgat tctcctgcaa cgatattact catgtaataa gggccaaaag
    15361 tcactggatt tttacgtact tgatcgcttg attccatatc tttgattgct acaccttcta
    15421 acgtatgttt tggtgctgca tatgaccaga taccaccacc cgattgaagc atgcttgggt
    15481 ttacttcttt atattcgatt tcaactgttt tgtcgtctat ttttttgatt ccggagattg
    15541 tgtcggcact tccatcatga tattcttcca tacctacgat gttacggaaa gtgtcgtcat
    15601 aacggatacc agtgtaatct ttattcccaa tgatttcgta agggaaaatc acatcgtccg
    15661 ccgttacatc ttcaccatct gaccatttga cgttgtcttt tactttgatt gtcgcttttt
    15721 tcgtatcttc atctagatca agcgttgcag ctccgtcgtc tgtgattctg aaatcgtcat
    15781 cgtttgcaaa tagcggttca tgagaaggag ccataaaagc tgcatcatat gaatcttggt
    15841 aaaactccca ctggaacaat cctttaaact gactgtccgt tgcgaccgca gcttctaatt
    15901 cgccgtcttc gattgcatca tcattatttt ttgttgcaat tggaaaagtt aattcctctt
    15961 tgttattgcc gttgcctcca gagttgtttc cagaggtatc tgtacctccg cccccgcatg
    16021 ctgcaagaac gacagcagac atcagcgctg caaatccaaa aatcgttttc ttcattccta
    16081 tttccccctt cgcttctata ctagcctaac cgttggcgcg catctgcaga acgcttgaat
    16141 gcttgtccaa cataattgat actcaacatc aaaactaaaa taaggattga tgcgggtaac
    16201 cagatccatt gccgattcac taagacttct ccgttacttg cataaccgat caatgtacct
    16261 aaactaggta catttgccgg caagccgaaa cctaagaaag tcaaagttgt ctcgataccg
    16321 atgtttgccg caaagttcat cgtcaaatta gtaatgatta atgaacttag attcggcatg
    16381 atttcgcgga acatgatttt aaaatcattg gttcccattg tttttgaggc actgacataa
    16441 tctcttcttg cttcagataa tgtcttgctt cggaataatc gggctttagc tacccaataa
    16501 aatgcactca tgatgaatat aaaggaccag acattgtatc tggaaataat cgatacgaag
    16561 acgataatga tcagcatgat cggtagaatc atgataaaat caacgatccg catcaagatg
    16621 ttgtcaatga tccctccgta gtagccagag atgatcccta atccaacccc gatgatcgaa
    16681 gtaaggatcg tgatcgcaaa tccgatcgta acagagtttc tagcacctat gatcaattgt
    16741 cctaatacat cacgtccgcc ttcatctgct cccagaatga acttttgtcc ttgtgtcgaa
    16801 actgttcctg gtgcagcgta tttgtcgaag atactaacat acatcacttt gtcttgatcg
    16861 gtcagcatcg caccgataaa gatcactaat aataaaacca ctagcaaacc taatgaaaaa
    16921 atagcaattt tatctttttt aaattctcga gcaatcattc gtatccccat aggaggaata
    16981 ctttcttgaa ctgctgtatt gtttttttca ctcatcgatc cattctcctt tctgattggt
    17041 tttattgaat ccgaatacgc gggtcaacga tactcatgat gatatctgaa agtaatgtcc
    17101 ccactagcgt cgcgattcct aaaatcaata ctaaggatgt gatcacggta tagtcccgtt
    17161 ggccgatgga atcaataaat agttttccga ctcccggata accaaaaatc ttttcgataa
    17221 ctacagaacc agcaatcaac gaagtgattt cataccccat ttgagaagcc atcggcaacg
    17281 ctgcatttcg gaaaatatgt cgactgaata ctttattcgt cggtacccct ttggaacggg
    17341 ctgttctgac aaaatcgagc gatttcgaat cgattacttc acttcgcaga tattggattg
    17401 tgacggcagt cgccaaaaat gcttgggtaa ttgatggtaa cagaatatat ttcaaacggt
    17461 ctatccattg agtgataaat ccaccttgca aacctagtgt ttgtgatcca cttgttggga
    17521 accagcctaa acgataacca aaaacaaata gcatgatcaa gccgaagata aataaaggaa
    17581 cagcaaaact aaagaagttg tagattacga caatcttatc caagattgaa ttttgatacc
    17641 ggccagcaag catccccaga ggcacagcaa tcaaataagt aaggacaact gtcaagatcg
    17701 atagatataa agtgttgatg atccgttcgc cgatcaagtc agcaacagag cgtttgtata
    17761 agaaactttg accaaaatcg ccttgcaaag catttccgat ccaacggaag tactggacat
    17821 accatgggtc attcaagcca gctgcttgtc gcatctgttc gatcactgcc ggatcctgat
    17881 ttggattgat caaaccggta aatggatccc ccggcatcgc ttgggctaat aagaagatca
    17941 gaacacttaa aataataact tgcgggatca tcaataaaac acgtcgcaga atcgttttcc
    18001 acattagctt gcacctccat ctttcaacgc cactttgtgt gtagttgtga tcgtacgcaa
    18061 atcgtagact cgaccagtcg catcatagta ttctttttgg ttttggatat actcttgttc
    18121 tactcttcgt cgattttctt tgtggctttc cctattttct acatcgattt gcggaatagc
    18181 tgaaagcaga cgtttcgtat agatatgtcg cgggtcatta taaatgtctt ctcttgtacc
    18241 gatttctaca aatcgacctt tgtacataat tgcgatattg tcgcacatgt gttttactac
    18301 acctaaatca tgtgaaatga ataaatagct gaggccgtat tcttgctgga ttcgtttcat
    18361 gaaattcaat acttgcgctt gaacagatag atccagagca gatacaggtt catcggctac
    18421 gatcagtttt ggattggtag caacagcacg ggctactcct agacgttgac gttgtccgcc
    18481 tgaaaattca tgaggatatt tatatagcgc atcttccggc atcccaacga tatctagcag
    18541 tccttttacc tttttctttt cttcttgatc gcttaaccgt tcaaaatttc ggatgggttc
    18601 agcaatgata tccaacactc gctttttagg attcaaactg gacattgaat cttggaagat
    18661 catctgcaca tccttgttat aacccatttt tttgcggttg cttcgcttcg ttacatcttt
    18721 tccttcatac atgatttgtc cggctgttac cttttctaat cccacaacgg cttttcccgt
    18781 agtagatttt ccagagccag actctcccac taaaccatat gtctttcctt tttcgataat
    18841 aaaatccaca ccatctacag catatacatg atccgttact cgattgaaga atccactgcg
    18901 aatagggtaa tgaactttca ggtctttgat tgtgattaat tctgtcatct acacctctcc
    18961 tttttcgtct ctaaagtgga aatgcttata gcaggagcaa cgtaccaagt ggttcggtgc
    19021 gacttcatga aggataggct cttcttcatg atcgcttgct ggtatccatg gaatacgtgg
    19081 tgcaaaccga caacctttac gaggcatgtt tttcagagaa ggaacgacac cttcgatcac
    19141 atggagttct gcatcatgat ttccttcttg tggtatagag ttcagtaaag agcgtgtata
    19201 aggatgctta ggatgatcaa acagttcttt tacacctgct acttcaacga actgtccgcc
    19261 atacatcact gccactcgat cagccatttc tgctacgact cctaagtcat gagtgatcaa
    19321 gatgatccct gtttttgttt cttcctgaag gtctttcagt aaatcaagaa tctgtgcttg
    19381 gatggttaca tctagggctg tcgtcggttc atctgcaatg acgattggag gtttgcaggc
    19441 gattgcgatt gcgatgatga cacgttgtct cataccgcct gatagttcat ggggatattg
    19501 gcgtccaact ctcgcaggat taggaatccc tacttgttct agcaattcta gtgcacgagc
    19561 ttgacgctgt tctgcattca tctttgtgtg ataggaaagt ccttctttga tctggtcttc
    19621 gattctcatg agaggattta atgcagacaa cggatcttgg aaaatcattc cgatatcatt
    19681 tccgcggatt ttattataga gcgtttcgtt caggtcgatt aaattcagat cgttatataa
    19741 aatctcacct gtaatttttg tgttattagg atcaagtaat cccataatcg tagtagccaa
    19801 ggtacttttc ccgcacccag attccccaac gattgctaaa atctcgtttt tgtctaaagt
    19861 aaatgaaacg tcatctactg catcgtaata gtcatctttg agtcggaatc ctgtatgtag
    19921 gttctgaact tctaataatt ggttatcgga caatcgaata cccctctctg cttcctaaat
    19981 ggaagttttt cttaataaaa aaggacatta cacagaataa atcgttttat aacaaaggtt
    20041 attct
//
LOCUS       DAZKOM010000047        19555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000047 DAZKOM010000000
VERSION     DAZKOM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.047"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..235
                     /locus_tag="ACG8NF_001618"
     CDS             <1..235
                     /locus_tag="ACG8NF_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472104.1"
                     /translation="SIGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIID
                     VSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(439..804)
                     /locus_tag="ACG8NF_001619"
     CDS             complement(439..804)
                     /locus_tag="ACG8NF_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472105.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1012..1494
                     /locus_tag="ACG8NF_001620"
     CDS             1012..1494
                     /locus_tag="ACG8NF_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472106.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1691..2581
                     /locus_tag="ACG8NF_001621"
     CDS             1691..2581
                     /locus_tag="ACG8NF_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0472107.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2906..5377
                     /locus_tag="ACG8NF_001622"
     CDS             2906..5377
                     /locus_tag="ACG8NF_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0472108.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5480..7216)
                     /locus_tag="ACG8NF_001623"
     CDS             complement(5480..7216)
                     /locus_tag="ACG8NF_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0472109.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7213..7917)
                     /locus_tag="ACG8NF_001624"
     CDS             complement(7213..7917)
                     /locus_tag="ACG8NF_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0472110.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8048..8551
                     /locus_tag="ACG8NF_001625"
     CDS             8048..8551
                     /locus_tag="ACG8NF_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0472111.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8511..8849
                     /locus_tag="ACG8NF_001626"
     CDS             8511..8849
                     /locus_tag="ACG8NF_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0472112.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8870..10288
                     /gene="ffh"
                     /locus_tag="ACG8NF_001627"
     CDS             8870..10288
                     /gene="ffh"
                     /locus_tag="ACG8NF_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0472113.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10400..10978
                     /locus_tag="ACG8NF_001628"
     CDS             10400..10978
                     /locus_tag="ACG8NF_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0472114.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10982..11503
                     /locus_tag="ACG8NF_001629"
     CDS             10982..11503
                     /locus_tag="ACG8NF_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0472115.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11582..12136)
                     /locus_tag="ACG8NF_001630"
     CDS             complement(11582..12136)
                     /locus_tag="ACG8NF_001630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0472116.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12389..12664
                     /gene="rpsP"
                     /locus_tag="ACG8NF_001631"
     CDS             12389..12664
                     /gene="rpsP"
                     /locus_tag="ACG8NF_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0472117.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12676..12927
                     /locus_tag="ACG8NF_001632"
     CDS             12676..12927
                     /locus_tag="ACG8NF_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0472118.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13052..13843
                     /locus_tag="ACG8NF_001633"
     CDS             13052..13843
                     /locus_tag="ACG8NF_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472119.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14013..14534
                     /gene="rimM"
                     /locus_tag="ACG8NF_001634"
     CDS             14013..14534
                     /gene="rimM"
                     /locus_tag="ACG8NF_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0472120.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14524..15285
                     /gene="trmD"
                     /locus_tag="ACG8NF_001635"
     CDS             14524..15285
                     /gene="trmD"
                     /locus_tag="ACG8NF_001635"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0472121.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15421..15768
                     /gene="rplS"
                     /locus_tag="ACG8NF_001636"
     CDS             15421..15768
                     /gene="rplS"
                     /locus_tag="ACG8NF_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0472122.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15900..17036)
                     /locus_tag="ACG8NF_001637"
     CDS             complement(15900..17036)
                     /locus_tag="ACG8NF_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0472123.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17152..17718)
                     /locus_tag="ACG8NF_001638"
     CDS             complement(17152..17718)
                     /locus_tag="ACG8NF_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0472124.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(17854..18507)
                     /locus_tag="ACG8NF_001639"
     CDS             complement(17854..18507)
                     /locus_tag="ACG8NF_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472125.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18557..18985)
                     /locus_tag="ACG8NF_001640"
     CDS             complement(18557..18985)
                     /locus_tag="ACG8NF_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0472126.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18990..19364)
                     /locus_tag="ACG8NF_001641"
     CDS             complement(18990..19364)
                     /locus_tag="ACG8NF_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0472127.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
BASE COUNT         6316 a         3509 c         3834 g         5896 t
ORIGIN      
        1 aagtataggt tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca
       61 aactattttt tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt
      121 gaatattata gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc
      181 aattattaca caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa
      241 ctagtgatag ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga
      301 tagataaaat gctgatattt cagcattttt taagagtata aaagagaaaa aaagtaataa
      361 atgtatacat gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg
      421 tacatatatg gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca
      481 taacgaccca aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt
      541 cggttttaaa ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga
      601 agtgacagta gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa
      661 ataatatcca gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata
      721 acgaaaatca agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag
      781 gtttttccag caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt
      841 agttttattg taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa
      901 atgaataaac tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag
      961 atagaaaatt gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga
     1021 aaagcagtaa tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct
     1081 tttacatggg taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc
     1141 ggatttgtgg tttctaggaa ttttccagct ttgactgttt tctatttagt gataagtgta
     1201 ggtctcttat taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct
     1261 tgcttattag tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat
     1321 ggtttgagcg agagtggatt gaaggattca gcttattcct ccatgtattt cttgacgatc
     1381 aaaccatggt tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg
     1441 acagaaatca ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg
     1501 atcatcagta aaaaagcata tgagctgcgt aaaatgcatg tttctcatat gcttttttta
     1561 ttattatgta aagaatgcga aaaacgttta catgattttt acataactta actatatttt
     1621 tacagcaacc atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag
     1681 gagcaataaa atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg
     1741 atgcggctca ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc
     1801 aaatgaacaa gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc
     1861 agcacaagaa agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg
     1921 aagcggaact ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt
     1981 atttgcagaa gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt
     2041 ggtcgggatg gcgccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga
     2101 attgattgat atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca
     2161 aaaaatcaac gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg
     2221 gggactggat ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg
     2281 gcagctggta agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga
     2341 ttccacacaa gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa
     2401 cagctgggga atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt
     2461 ccaaaaattc ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt
     2521 gggctattta ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata
     2581 actagcagat aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc
     2641 cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga
     2701 gctaataccg agggtcagct gattaaacac cgtttattcg aaaaaaagag ctacgacata
     2761 gaactgtcgt agctcttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat
     2821 gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga
     2881 aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa
     2941 gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg
     3001 attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga
     3061 tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt
     3121 gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt
     3181 tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa
     3241 gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gggacgggat
     3301 agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt tagagactgg
     3361 acatcaaatt ccagcagatg cagaggtcgt ggaaggaaaa ctgcaagtta atgaggcttt
     3421 attgacaggc gaagcagatg aaataaccaa agaaataggc gatccattaa tgtccggcag
     3481 tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat
     3541 ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc
     3601 ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct
     3661 tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga
     3721 agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc
     3781 attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga aaagcattga
     3841 gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa
     3901 aatgtctgtt caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca
     3961 attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga
     4021 agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt
     4081 ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc
     4141 gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc
     4201 tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt
     4261 attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga
     4321 agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg
     4381 agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca
     4441 gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac
     4501 agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga taaagcgtaa
     4561 caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga
     4621 ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt
     4681 acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa
     4741 caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc
     4801 actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat
     4861 tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg
     4921 aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga
     4981 tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga
     5041 tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat
     5101 ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt
     5161 tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact
     5221 tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt
     5281 ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagc
     5341 taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt
     5401 aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg
     5461 cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc
     5521 caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct tttacaatcg
     5581 ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg cggtaaaaac
     5641 gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa
     5701 acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt
     5761 ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa
     5821 atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat
     5881 aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat
     5941 tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat
     6001 ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca
     6061 gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt
     6121 tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca
     6181 cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg
     6241 tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt
     6301 gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt
     6361 gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat
     6421 cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct
     6481 cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac gcattaatcg
     6541 tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat
     6601 tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga
     6661 tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc
     6721 aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa
     6781 tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga tcctcattca
     6841 aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt
     6901 cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc
     6961 cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag
     7021 ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt
     7081 tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca
     7141 attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa
     7201 tttttttcca tttcatcgtt ttggttcctg aaacttatag ccaaatcctc taatagtcac
     7261 caagtacttt ggatgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac
     7321 atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc
     7381 tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg
     7441 tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat
     7501 ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat
     7561 ccgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa
     7621 atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt
     7681 cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc
     7741 atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa
     7801 ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt
     7861 gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca
     7921 aatctccttt aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc
     7981 tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga
     8041 gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca
     8101 gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg
     8161 tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa
     8221 tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac
     8281 ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga
     8341 tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacggaag
     8401 tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa
     8461 aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg
     8521 aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac
     8581 aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag
     8641 aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg
     8701 aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa caggtattgg
     8761 aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg
     8821 tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga
     8881 aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc
     8941 agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt
     9001 taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga
     9061 agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac
     9121 gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat
     9181 catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta
     9241 tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc
     9301 tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg
     9361 aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa
     9421 aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga
     9481 tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc
     9541 catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac
     9601 aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg
     9661 ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga
     9721 agtattccac ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt
     9781 gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg cacagaaaat
     9841 gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat
     9901 gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga
     9961 aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac
    10021 gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc
    10081 cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag
    10141 aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt
    10201 tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa
    10261 taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa
    10321 atgtctactg ccggagcttt tctatcagca atttttattc tttactaata caattactct
    10381 aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca
    10441 atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt
    10501 gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc
    10561 attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg gagaaacaaa
    10621 aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc
    10681 cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta
    10741 tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt
    10801 cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa
    10861 atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca
    10921 ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag
    10981 catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac
    11041 ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt atgaagcttt
    11101 ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata
    11161 tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca
    11221 attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct
    11281 acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct
    11341 attggaacat caaatgattg atgaatggta tatccagatt acccctgtat tattagggga
    11401 cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac
    11461 acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa
    11521 aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt
    11581 tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac
    11641 ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat
    11701 atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg cgtagatggt
    11761 ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat
    11821 gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg
    11881 ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt
    11941 cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt
    12001 aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg
    12061 caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca ataaaaaatc
    12121 caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat cttacaaaag
    12181 ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa gatagattac
    12241 ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat cgaatttaat
    12301 caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact acatcttgtg
    12361 aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa aacgtatggg
    12421 ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc ctcgtgatgg
    12481 acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag aagtagtttt
    12541 aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg acactgtacg
    12601 taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat tctcaaagaa
    12661 ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt tcgtccgcta
    12721 gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt ttacgagtac
    12781 aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg gcgtgttgct
    12841 aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa agtgcgtttg
    12901 aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt tcgagtgcag
    12961 cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag caagaaagcg
    13021 tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat ttattccatt
    13081 tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata cacatctatc
    13141 aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa cggcttccac
    13201 cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag accagttgaa
    13261 agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag gctatctcaa
    13321 tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa cagatcgtcc
    13381 cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct atctatatca
    13441 atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg agatcgacac
    13501 aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag gtgcagcagg
    13561 ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg ggaataatat
    13621 agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg aagaaaacag
    13681 tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata cgacgaatcg
    13741 agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc atcaagatcc
    13801 tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc gcgctgatct
    13861 gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc cgcatttttg
    13921 cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca catgctaaaa
    13981 taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat gtaggaaaaa
    14041 tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg gatttcccag
    14101 aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa gcacctgtgg
    14161 aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt tttgaaggac
    14221 atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg tcaaaagaca
    14281 actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt ctgcatgtat
    14341 tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt gccaatgatg
    14401 tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc gcttctgtcg
    14461 tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa ggactgattg
    14521 acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc tatgggggaa
    14581 tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc taacttccgc
    14641 gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg tggagcaggt
    14701 atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa agctgcaccg
    14761 gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga tcagaaaatg
    14821 gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta tgagggatat
    14881 gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta tgtccttact
    14941 gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt gccagatgtg
    15001 ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt ggaacatccc
    15061 caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt gacaaatgga
    15121 aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac ctatcaacgt
    15181 cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt tttagaagaa
    15241 atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt tctaaaatct
    15301 tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag cacttaacta
    15361 ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga gaagaaaacc
    15421 atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat cccagcgttc
    15481 cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg tgaacgtatc
    15541 cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga aacttataca
    15601 gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca tacaccacgt
    15661 gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt gtactaccta
    15721 cgtgcattac acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc atcgttatca
    15781 gacagagaaa gaacccttgg tattcaaggg ttcttttttt attatctgtt tttttatatt
    15841 aacgatttct atttggtaca tttttgatta ataaaaaggg gcaaaaaagg ggcaacaagt
    15901 tacagttttt ctatatcttc tttcaatttt tcttttactc cttttgtcac atgcaaataa
    15961 attttttcag tgatatcact attttcgtga ccaacgcgat cctgaatggc atacagagga
    16021 gttcctagtt ccgctaattt tgaaatatgg gtatgtctaa aaatatgtga actaagtttt
    16081 ttatcaatct tcatgtcagc tttatgactt ctcaaatagg tgttaattgc tgttagttgg
    16141 aaaggagttc ctttcgttgt ttgaaataga aattgaccat ttggatttaa ttctagaagc
    16201 tcattgtaaa tagctatcgc tttttttggt aaatcaattt ctcgcattcc ggcagctgtt
    16261 tttgtagaat cagatttttt catatcagct attgaacgtt ctctatacat catcgtccca
    16321 tttataacta ctgatgcatt attattggta atgtgtacat catctttact taatgcgatt
    16381 gcttcaccag acctcatccc agtcaaatat agccactgga aaaggagaga atatctttta
    16441 ttgtgtgaag ttgtgaaatc tactagtcta ttatattcat catcttctaa aaatttatct
    16501 ttaattttta tcgtttttga ctctctttta taatcaataa ctacttcatc gataggattt
    16561 ttttctacat aacctttttt catagcatat gaaaaaagaa gattcaattt ggatttaatt
    16621 acactgacgt acttatttga caagtcatct ttatatatca tgtcttcaaa aatattattc
    16681 aaatctattg tattaatacc agaaacgatg taagtctcag gtatcttctt ctttatagtg
    16741 tttaatatat tattcgtagg ataatatgta gattccttaa cttgtctttt gtatataact
    16801 aaccattctt caacaagctc atgaaatgtt agatcaggct tatgaagtgt tttttcgttt
    16861 aattttatat caattttttt atttaactct agcaatgcca ctttctgagt ttctcgagat
    16921 ttatttttat aagtaatgct cacttttttt ctttttctag tttttggatc aatatatcgc
    16981 tcattatatt tatatacttt ttttccgttt ttatcagttt tagtttcgat ccacattgtt
    17041 ttcatctcct atttgttgct ataataggaa tagataagta agccgattat agcaggttta
    17101 tttttcatca cgtccacaaa ctttggcgag ggaggggcgt gtttttgtta gttattgtgg
    17161 taggttatct aaagcgtatt gtgcttgctc atttgtaaaa ccttcaaata taagttgatc
    17221 gtacaattgc gaatctgaca ttgatgccca atcatcgtaa ctttccgctt tagccaaagc
    17281 ttgttcgttg taatcgacat caacattagc cagcgcataa tcgattgcat cacttggata
    17341 ttcttcaaac tctagttgtt cacgcaaacc ttgttcagac ataccagccc aacctagata
    17401 gctttcagct ttgctcaatg catttctgta ttcacgagga acactttctt cttgagtaac
    17461 ttcttctata gtagtttcag tagtttcagt ttcttcttca acgactgtag attctgctac
    17521 cgattctgca gaagacactt ctgtcacgct ttctttagtt gtttcagcga cggtattttt
    17581 agcatcatca ctgcctcctt gcgaacctat agcaataaac accacaacaa ccgctaacaa
    17641 ccaaaaccat acccgtttat aaaacggttt cttaacttta tacattttcc catcttgacc
    17701 cataactttt tttgccattt aaatatccct cgcttcttgt tataatatat ttgtgatctc
    17761 agaaatgagg tatgagtccg tgttgcagca cgggcttttt tctttataac ttttttagag
    17821 attataggca aaatagtagg gcataaaaat gtattattga attccgtatt tagaaaatcc
    17881 taattgaact tcaccggaag tcttttgctg tgttgtacgc aatgcttctt cttcagacat
    17941 tccattctgt actttccatg caacaggcga catcccgtat ttgttaacaa aatcagtaag
    18001 tgataaagtg tcagcgtctt gctgagcgct tgtttgttgg tcttctggat tctgttgaga
    18061 tgctgcttgt tgttctttct gatcttgact gataatattg ccagcatcat ctgtagtcaa
    18121 tccattttca taaagggcca cgccgaaagc ttcccactct ttgttggacc aatttgcacg
    18181 atcagctgga gttgactgta aagtgcgttg tttcatctgt tcatatgttt cttcttgagg
    18241 tgcggtttgg attgtactct gactggagct tataactgtt gggctaggtt ccgccgtagc
    18301 ttggttggag cttgtaactg ttgaactaga atctgtttta gatgtagatt tgctagtaga
    18361 tgaactggtt tcagttgttt cttttgtttt acttattttt gtttcttggt tagaagtggt
    18421 atctgttgat tcagcttttt tattatttga acaagctgaa agtagcagag cagtacttaa
    18481 caacaacata acgctaactt ttttcatttt ataattcctc tttctcgttg taatatgtgt
    18541 gctaacacgg gcttttttat ataagaaaac gataagcgct ttctggaagc ccgtaaagat
    18601 tctttaattc ctcgattttt ttaggatatt gatcattatc ttctttataa agagaaacaa
    18661 tgagattagc agcaaagcaa ttagcttcgc tttcagattt gcttctagat gttcttgttg
    18721 atacataata gctggataag ccacgatgaa aaatagcgtg acctaattcg tgagcgcaaa
    18781 tgtagaatct ttcctcagag tctcgcagtt catcatttaa aaagattatc gcacgatctc
    18841 taatttcttg aaactgcccc ttgggattct cgataaaagg aacgtattga attttaatgc
    18901 ccatcttttc acaaatataa aaaggattag cggactggta tttccgcttc aactcctcga
    18961 ctaaattaat cgtatccatc tccataagct cacatctttt tgcctttttc tttgtcttct
    19021 ttcacaatat cccagaaagt cgctatcagg atatctttta cgcgctgtat ttgttcgggt
    19081 gtcaatgttt ccccaccata agacatatta acatttgagt ctagtagttt atcaagttca
    19141 accacttcct cttttgtagc ccacttggga acattattat ttcccaataa ataatcagtt
    19201 gtgacaccaa aataatcagc aactttcttt aagttctcag attttggcga ggctttatcc
    19261 catcttctta tttgtccatt agagatgccc acctgtcttt ctacttctgc tatagtcaca
    19321 tgcttttcgt ctgctaattc tttaatctta gtaactaaac tcattattat caacctttca
    19381 aagctgaaaa gaaaataaat agcttaaaag ttatattttt ggttgacaat tagcttttaa
    19441 gctagtatat ttaattcgta agctaaattg ttagctaaat aagagcaaca aaaaactcta
    19501 ctaatttaaa acattctctc ggtcgccaaa cttagaaatg ttattttaga ggctt
//
LOCUS       DAZKOM010000048        19387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000048 DAZKOM010000000
VERSION     DAZKOM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.048"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            756..1418
                     /locus_tag="ACG8NF_001642"
     CDS             756..1418
                     /locus_tag="ACG8NF_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472128.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1497..4724
                     /gene="fss3"
                     /locus_tag="ACG8NF_001643"
     CDS             1497..4724
                     /gene="fss3"
                     /locus_tag="ACG8NF_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0472129.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            4838..5152
                     /locus_tag="ACG8NF_001644"
     CDS             4838..5152
                     /locus_tag="ACG8NF_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0472130.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5165..5539
                     /locus_tag="ACG8NF_001645"
     CDS             5165..5539
                     /locus_tag="ACG8NF_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0472131.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5549..5893
                     /locus_tag="ACG8NF_001646"
     CDS             5549..5893
                     /locus_tag="ACG8NF_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0472132.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            5964..7316
                     /locus_tag="ACG8NF_001647"
     CDS             5964..7316
                     /locus_tag="ACG8NF_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0472133.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7376..8518
                     /locus_tag="ACG8NF_001648"
     CDS             7376..8518
                     /locus_tag="ACG8NF_001648"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0472134.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8543..8797
                     /locus_tag="ACG8NF_001649"
     CDS             8543..8797
                     /locus_tag="ACG8NF_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472135.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9053..10237
                     /gene="mobT"
                     /locus_tag="ACG8NF_001650"
     CDS             9053..10237
                     /gene="mobT"
                     /locus_tag="ACG8NF_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0472136.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10234..10371
                     /locus_tag="ACG8NF_001651"
     CDS             10234..10371
                     /locus_tag="ACG8NF_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0472137.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10376..10597
                     /locus_tag="ACG8NF_001652"
     CDS             10376..10597
                     /locus_tag="ACG8NF_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472138.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10610..11110
                     /locus_tag="ACG8NF_001653"
     CDS             10610..11110
                     /locus_tag="ACG8NF_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0472139.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11207..13054
                     /locus_tag="ACG8NF_001654"
     CDS             11207..13054
                     /locus_tag="ACG8NF_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0472140.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13057..13953
                     /locus_tag="ACG8NF_001655"
     CDS             13057..13953
                     /locus_tag="ACG8NF_001655"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0472141.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14003..14392
                     /locus_tag="ACG8NF_001656"
     CDS             14003..14392
                     /locus_tag="ACG8NF_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0472142.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14379..15878
                     /locus_tag="ACG8NF_001657"
                     /pseudo
     CDS             14379..15878
                     /locus_tag="ACG8NF_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16626..18452
                     /gene="ltrA"
                     /locus_tag="ACG8NF_001658"
     CDS             16626..18452
                     /gene="ltrA"
                     /locus_tag="ACG8NF_001658"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0472143.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18565..19387
                     /locus_tag="ACG8NF_001659"
                     /pseudo
     CDS             18565..19387
                     /locus_tag="ACG8NF_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6552 a         3169 c         4271 g         5395 t
ORIGIN      
        1 ttacaggttg gttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga
       61 atttatcttt tttgctgctt tgctcataaa agtctgcata ttcatcactg atattttttc
      121 taataaagtc ttccatcata aaaatcacct caaaacgatt atacgaactt acgttctcgt
      181 tgtaaagcga tatttgaggg gcaaaaaggg ggcaaaaata cgtttacctt tgatatatct
      241 cagattgaac atagtgttta ttatatgtcg gtattgttga tttatagctg ttttgagggt
      301 attagataca taatagaata tagtggtttt gtttacgtgc attacacgga aaagctgctc
      361 gtatcaaaga aatcagaaga taaacacgga aaacttcctt ggaaatctga aatttgtgaa
      421 aagtccttgt atatcaaggg ctttttcttt tttctctatc acagttttaa gccatttttg
      481 gctgttttgg accatttttc aatcatattc ccttaaaaat tgtcctagat tgtccgacat
      541 tgactatcag tgtcgtcaaa atagggcaaa atttagtagt cagcttgacg acaaatataa
      601 tacgaaaaat accaaaacat ttaccgaatt tagcaacttt tgaacccaag tttctcaaag
      661 ggtcagctat tagcaaaaaa gacaaaacat gtacttttct actaaaaagt tgatgtgata
      721 ggcttctatc taaataacta ttaggagttt ttatcgtgaa gcaaaatgat ggacgtatca
      781 tttggaaaaa tcaaagtgag ttaaaactga ttttaacaat taatgaattc atcgaaaaac
      841 acggaattac atctagccgt caataccaga aaaagctatc agaaaatcca aattctgcac
      901 caagtatgtg gttcattaac aaaaaatatg gatcatggga gaatttattg attagtatag
      961 gacgagaaaa tactgggtat ggaaaatggg ctagaatgtc agaacaagaa ttattggaaa
     1021 tagtagaagc ctttattaag tgcgagaaga taacctctca acgaatgtac gaacaaaaat
     1081 cagtagggaa gaatattcct tcacttagta ccattaaaaa gatgttagga gatatacgtc
     1141 cactttttaa agaaaaaaat gatggatctc gatttactga tttcgaactg ctgttagaac
     1201 taaaaaatga aataatccga ttaaaactac aggatgattt atcaatgaca aagtttcgaa
     1261 aactagttca atccccaaaa ctaccatcag ttgatacaat tatgaaaaga acgaacaaaa
     1321 attgggagga attgatggca gagattggat ttgattatcg aagaatcaaa atttataagc
     1381 agagaaacaa cctatccaag acaaagaaaa ctaaatagta tcactctaaa tcagacgttc
     1441 aaaattaaaa tttggacgtc ttttttatta cccaaaaacg aaaggagatt tattttatga
     1501 aaaagccaag atttaagaat tggcgtttgc tcgcaacact gagtctatta tgccaaacaa
     1561 ttggtggatc gcttggccca acgattgcct ttgctgatga aattactcat ccacaaacgg
     1621 taactgtgga attggatttg gctcaccagt atgctgtcga aggtacattt agcgatggtc
     1681 gtcccatgtc agaagttacc gttccgcact atgcagttta caatggtgtg aagcaagata
     1741 ttttctgtat tgagccaggg gtgccgattt acaatgagtt cactcctggt tatgagaaaa
     1801 atccattgcc tgatatgtcg gagaaagcga aattagtttc cgttttatgg aaggaagctg
     1861 gtacggatgt ggatacacat attgtggccc agaagatgat ttggcaagaa gttaatggct
     1921 acacgcttca ctcaattaaa cgttctgatg gtagtgcagt aaatatcgca gccattgagt
     1981 caaaaatcaa tcaagccatt gcggattatc aaaagaaacc aagcttccat aactccacag
     2041 tgaaaaccgt gttaggtcaa tcgactactg tgacagatac gaacggcttg aatttatcgg
     2101 agtttgatga agttgtcgag aatatcgcaa atattgatta tcgtgtgaac ggcaatcaac
     2161 tagtgattac tccaaatgcc aattcaaaag agagtggtga attaacccta aagaagtcag
     2221 ctaatacagg aacaccggta gcttataaaa tggctggtca acaaactttg atggcggggg
     2281 caattgataa gcccaatacc tacacaatca aaattgatgt ggaaactgag ggttctttga
     2341 agattaaaaa agtcgataaa gaaacaggaa atgttgtacc gggaaccgtt ttccatttag
     2401 actttggaaa aacattacct gcaaaagacg tgaccaccga taaagaaggc attgcgacat
     2461 tggatgagat tcctcatggc gcgaaagtaa ccattactga aaaatcagtg ccagctcctt
     2521 atacgattga cactacacca atcaccgcaa ccattaaatc tggtgagact atttctgtga
     2581 cttcaaaaaa tactcgcgaa aaaggtcaaa tcattttgaa taaaatgggg gtggaaacag
     2641 gaactgatct ttggaacgac aaatactcat tagccggaaa tacgtttgcc attcgcaaag
     2701 acagtccaac cggtgaaatt gtccaagaaa tgaccacgga tgaaaaaggt catgcggaaa
     2761 caccaaaaga gattgccaat gcgttggaat tgggaaccta ttatgtgacc gaaaccaaag
     2821 ctagtaatgg ctttgtgaat acctttaaac cagtaaaagt cgaattgaac tatgtcaatc
     2881 aaaccgtggc tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatc actggtgaaa
     2941 ccactttgac aaaagaagac aaagataccg gtaatgaaac tcaaggtaaa gctgagttta
     3001 aaggagctga atatactctc tttactgcaa aagatggtca agcggtcaaa tggagtgagg
     3061 cttttaaacc agagttagtg aagggaacga aagcttctga tgaaacggtg actttgactt
     3121 tagatgaaaa gaatcaagtt gctgttaaac acttagcgat taatgagtat tactggcaag
     3181 aaaccaaagc acctgaagga tataccttgg atgaaacaaa gtaccctgtt tccatcaaaa
     3241 aggtggatga taacgaaaaa aatgccgtga ttacccgaga tgttacggca aaagaacaag
     3301 tcattcgctt tggctttgat ttctttaaat ttgctggatc agctgctggg actgccgaaa
     3361 ctggcttcaa tgatctgacc tttaaagtat cgccattgga aggaaccagt gagattacag
     3421 atgccgaaga tgaagcaatt acagcttata acgagcaact aggttttgat ggctatggca
     3481 agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaggct ccagaaggat
     3541 ttcaaaaaat tacgccatta gaaatccgtt ctacttttaa agaaaataaa gcggattaca
     3601 ctaagagtga atacattttt acgattaccg aaaaaggaca aaaacaacca attaaaacgg
     3661 tgaccgttcc ttacgagaaa ctgacgaata aagcattttc tgttagcttg aatcgtttga
     3721 tgctctatga tttacctgaa gaagaagata gcttaacttc tcttgggact tggaaagacg
     3781 gaggcaaaga attgacagac cttgattcta ccgagctagt tgataaattg agctataact
     3841 tgcatgaaat caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gaagcgacaa
     3901 aagctgccca agaaaaagat gaaaaagcca aaccagtggt aattgccgaa acaaccgcaa
     3961 cgttggcgaa taaagagaaa acaggaactt ggaaaatttt gcataaatta accgcagaac
     4021 aagttttgga taaaaccatc gtcttattca actatgtgta tgaaaacaag aaaccctatg
     4081 aagcaggtga tgatccagta gcgaaagatg ctagcttgaa caatcaagcc caaactgtca
     4141 attgtacggt ggaacgccat gtttccatcc aaacaaaagc ccacctagaa gatggttcgc
     4201 aaacctttac tcatggggat gtggtggata tgttcgatga tgtgtcgatt acccatgatg
     4261 tactggatgg ctcaaaagaa gcttttgaaa caattctgta tgcattactt ccggataata
     4321 cgaccaaaga aatttggaaa tctggcaaaa ttgattatga agtgaatgac aaagaattca
     4381 ctaaaaccgt acttgcggaa aaagtggaca caagtaaata tccagaaggt acaagtttta
     4441 ccttcaaaga aattaactat ggcaaagacg gaaacatcaa tgggaaacac aatgaggatc
     4501 taaaagaaaa aactcaaact ttaacaccta aaaaagttcc gacaacacca agtgcaccag
     4561 aacaaccgga aataccaact gttccaagtg actctcaaga atctagtcct acagtaaaga
     4621 cattcccaca aacaggggag aaaaattcta atgttctgct gtttattggg tttaccttga
     4681 tctttgcgac tgctgggtat tatttctgga atcgccgaaa ctaaggtgat gtgatgaaac
     4741 aactaaatag gaaaaaccaa cgtgcgcgac cgcctcctaa aaaaagtggt cgcgctattt
     4801 ttgtatccaa aactaaaaat gaaaagagga aaagaagatg gaattgaaat ttgttgtgcc
     4861 agatatggcc gaaacctttg gaaagataag gtatgccggc gaaggcgaag ttttaactga
     4921 aggatatggg cggaatacta cagtaattgg tcgtagttat catttgtatt ccagtaagca
     4981 acgagccgat gatattgaag tggtggtagc tgcggaagcc ggcgaaaagg attttgatca
     5041 agatcaacca ctaaaagccg tgaatcccca tttggttgcc aaaggctatg agattgagaa
     5101 tcgtgggttt accgactacg tgttgtatgt cgatgattta gtgaaagcat aggaggaaaa
     5161 gaagatgaga ttaacagaag gtattgttgt agattcagga ctaacgtttg ggaaattacg
     5221 attttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacgg ttagtaacga
     5281 ggtaaaagaa cggacttata atttgaaatc ctctgcgcaa ggtcgaatga tccaagttag
     5341 tatcccagca aatgtaccct tacgcgagtt tgcctatgac gcagaggtag agttggtcaa
     5401 tccaatcatg gatacggtcg ccaattatgt tttccgagaa ggaactactg tcaattggtt
     5461 cattaaggcc gatgatttag tcttgaaacg acaaccagat cagggaaatt ctacaaacca
     5521 aaatgaagga aagaaatagg tgaagcgtat ggaaattaat gttgaacata ttttagattg
     5581 tctcgatcaa tatggaaaag gtgagctaac ggaggaacaa ctcactaaag cattaaccta
     5641 tgatgaaaaa atgtttctga ttatgcatca agggttactg gaagtaggta acgatgccga
     5701 agaagatttt gatgtactga attggttagg ggaagaagag tccttcttta tggttatcga
     5761 agtaaatgaa agtctatgtc gagaagccga aaccgtattg gaagaaattg gtgtggaaat
     5821 gccggatgcc attgaagttt ttttaaagca actagtggaa acgaaacaac tccctatagc
     5881 agtaaatgac tagcataact gcttgaaatt ggatcaatga aatcctatga agcttttttc
     5941 gattggaggt ggaaaccata aaaatgtaca aagggcatcg tatacgggca ggagatcagc
     6001 acttggttta tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg
     6061 tcttttattt tcaagagctt agacaatttg atatttctaa gctatcactt tctactatag
     6121 aaatcgttcg ttctataaaa gatttaatct atttgctagg aagtctcgtt ctttcaggaa
     6181 gcacgatgtt gctttatata cacttttttc aggatcattg gcgcagtttg tggcatcgac
     6241 aaaagctagc ccggatgatt ctagaaaatc attggtacga agtaaaacaa actcagagtg
     6301 aaggattttt caaagacctc aatagtagtc gaaccaaaga gacaatcagt tacttcccca
     6361 aaatctatta ccgtatgaag gatggtttac tttctattcg cgttcaaatt tcactgggaa
     6421 aatatcaaga tcagctttta aagctggaaa agaagttaga aagtggtcta tattgtgaac
     6481 tagtagaaaa ggaactcaaa gactcctatg tggagtacac tttactgtat gatataattg
     6541 ccaatcgaat tgggatagac gaagtagtgg cagaaagtgg ggctttgcga ttgatgaaaa
     6601 atcaagtatg ggcttatgat tctttacctc atatgttaat cgctggcggc acaggtggtg
     6661 ggaagactta ctttttactc actatcatcg aggctttatt aaaatctgat gccgagcttt
     6721 ttgttcttga tcccaaaaat gcggacttag ccgatttagg tacggtaatg cctcatgtct
     6781 attcccaaaa ggaggaaatt tctgcttgtg tggaagattt ctatgagcgc atgatgactc
     6841 gtagcaaggc aatgaaagaa atgcccaact acaaaacagg agagaattat gcgtttcttg
     6901 gacttccacc aaacttttta atctttgatg aatacgtggt ttatatggaa atgttaacga
     6961 cgaaggaaag tgcggtgatt ttgaataagc tcaaacaaat cgtgatgtta ggtcgtcaat
     7021 ctggtttctt tctgattcta gcttgtcaaa gaccggatgc gaaatattta ggagacggga
     7081 ttcgcgatca atttaatttc tgggtggctt tgggtcgtat gagtgaactg ggatattcca
     7141 tgatgtttgg cgaggttgat aaaaaattct ttatgaagcg tatcaaaggt cgaggctatg
     7201 tagatacggg agggagtgtg attagtgagt tttatacgcc acttgtacca aaaggatatg
     7261 atttcttaag ggaaattagc gacattggtg gattaaacgt taatacggaa ggataaaata
     7321 atagtatgta gcctaggagt gtcaagaatc ctgggctttt tttacgaggt gatttatgac
     7381 atttttagat ttattcgctg ggattggtgg cttttgctta ggtatggaac aagcgggtca
     7441 ccagtgtatc ggcttttgtg aaattgatga ttttgctcgc cagagttata aggcaatcca
     7501 tgatacaagt aaggaggtgg aaatgcatga catcacaagt gtatcagacg agtttattca
     7561 atctctcgga ccagtggata tcctttgcgg tggatttccg tgccaagctt tttcaattgc
     7621 gggaaagcgg caaggatttt ccgatactcg aggtacttta ttctttgaaa tcgctcggtt
     7681 cgccgctatt ctccaaccta agtttttatt ccttgagaac gtccggggat tactcaatca
     7741 cgaaggaggg gctacgttcg agacgatcct ccgaacgctg gatggattgg ggtatgatgt
     7801 ggaatggcaa gtgcttaact caaaggccta cgttccccaa aaccgtgagc ggatcttcct
     7861 tatcggacat tctcgagacg catgtaccga acaagtattt cctatcattg gatcgtctcc
     7921 aacatctgat caaaacatca gaaacttatt aaatatcaac ccttccaatc gaggaatggg
     7981 gggacaagtc tatgggtcag atgacgtagc gcctaccttg accggtgatg aaggaatcaa
     8041 aatcgcattg ccggtagatg atggaatttc tgtagcgggt atgttactgg gaaattttga
     8101 acaaggcaat cgggtttatg agataactgg aacagcgcct actttgtcaa cgaaacaagg
     8161 cggaacgaag atcatgcttc ggaaaaacga aacagaatat caagaggtga agccagggaa
     8221 cagtgtgaac ctggcttttc ctaattccac aagtcgtcga ggccgtctgg gaaaacaaag
     8281 tgtgcatacc ttattaacag gagaccaaca agcagtcgtc acggatcagt atcaaattcg
     8341 aaagttaaca cctagggagt gctggcggct tcagggcttt ccggactggg cttttgatcg
     8401 agcttctcaa gtcaattcag atagtcagtt gtataagcag gctggaaatt ctgtaacagt
     8461 tccggtcatt tttgatattg ctagaagatt aaaggaggta aaaacagatg atttatgatg
     8521 agttgattgg ggaatctact gggtgattgg caagattcag tcagatccgg aattggaaga
     8581 agaactgtac cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc ctgatgatcc
     8641 gtatgtaatt gatgaggaaa cggatgctcg aatggagctg aatcaagtca tcagtgaatt
     8701 ggaacgaatt gcggactttg ctaaagaacc tgatatccgc caatacttgt ttgagataaa
     8761 agcagagttg gaaattgagg gtattaccgc ggagtaacgg ccaaataaac cactgatagg
     8821 cactgaccgc gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc tgtttatgga
     8881 cggcgttagc cggccatgat gtctcacccc ccgtatctaa caggggggta caaaaacaca
     8941 aaaaataagc aagcaacgat aaggaatggc tgtcctatca aggataataa gcaatttgtg
     9001 tagatgttaa ctgatttgaa aaagttaaca ccttaaattt ggaggtgaga acttggcaca
     9061 aaggaattta gattatcggc tattaaaaga ccggcgtaat gaatatggcg tttcacaaaa
     9121 taaattagct acgacttgtg gacttagtcg cccgtactta aatcagatag aaaatggtgg
     9181 cgtaaccgca tcgacaaaaa ccatgaggaa aatttttgac caactggaaa gtttcaatcc
     9241 cgagttacct ttaacgttac tgtttgacta tgtaaggatt cgctttccca caacggatgc
     9301 gaggaaaatc attcaagaga ttctccattt gaaatttgat tatatgctcc acgaagatta
     9361 cgccttttac tcctatcaag aacaatatgt tatgggagat attgtggtga tgttgtcaca
     9421 tgaagaagat aaaggcgtcc ttttagaatt aaagggtcgt ggttgtcggc agtttgaaac
     9481 ttttttactc gcccaaaagc gtagctggta tgactttttc gaagattgtc taaaagccgg
     9541 tggcgtgatg aaacgcttag acctggcaat caatgatcga gttggcctgt tagatattcc
     9601 tgatttaacg aaaaaatgtc agaaggaaga atgtatctcc ttgtttcgta ccttcaaaag
     9661 ctatcgttcc ggggaactgt tgaaacctga tgataaagat ggtatgggca atactttgta
     9721 tattggcagt cttaagagtg aggtctattt ctgtttgtac gaaaaagact atgagcaata
     9781 tatcaagtta gggattcctt tagaccagac cgaaacgaaa aatcgttttg aaatccgact
     9841 gaaaaatgat cgagcttatc atgcaattca agatttgtta aaaggtcgca gtattgaaag
     9901 taccacattt tccattatta accggtactt gcgatttgcg gataaggtgg aaggcaaaag
     9961 gcgaactaac tggcccttga atgaacaatg ggggcgcttc attggacgga atcagaagga
    10021 aattcaatta acttctgaac cgaagcctta taccattgaa cgaacgttga actggcttgg
    10081 acgacaagtc gcccctacgt ggaagatggc caaggagttg gatcggttga atcagacaac
    10141 gtacattcag gatatggtaa ataatactcg actatcagac cggcacaaaa agattttgga
    10201 gcaacaaagt atggcaattg aaaatttgat tatatgaaaa gaggaatcgt gatggaaatt
    10261 gtgaaagata ttttattggt gtttggtggc agtatgcttg gtgttactgt gatgtgcttg
    10321 atgcatgcaa gtgcagcggc ggaccgagca atggaaaaga aaggaaagtg atgaaatgaa
    10381 tttcggtcaa aatttgtatc aatggttttt aaccaatgct caatctttag tcttactagc
    10441 gattgtggtc attggtttgt ttttaggatt taaacgcgag ttttccaaac tgattggctt
    10501 cttggtgatt gctttgattg cggtaggtct ggtctttaat gcttctggtg tgaaggatgt
    10561 cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat
    10621 acgtgtttat attgcgaacc ttggaaaata caatgaaggt gagttggtgg gggcatggtt
    10681 taccccaccc gtcgattttg atgaagtgaa agaacggatt ggattaaatg acgaatatga
    10741 agagtacgca attcatgatt atgaactgcc ttttgagatt gatgagtata caccaattga
    10801 ggaaattaat cggttgtgtg gtttagcgga agagcttaaa ggaacgccaa ttggtgaggt
    10861 cgcttcagag attcaacatg ccttcttcaa ttctttcgag gaaatggtag agcatgtgga
    10921 tgatatcatc tattatcccg attgtaatga catgagtgat gtagcgtatt acttgattgt
    10981 agaagctggt gatttaggcg aagtaccaga acacttgaag acgtatattg attttgatgc
    11041 gtgtggtcga gacttggaga ttggcgggaa ttatttggtt acgagtcgag gggtttttga
    11101 actaatgtaa atcatttgtt agaattgacc agaaatttct atcgtacttt tacttttcta
    11161 tttgatatac tgaaatagaa aatatttgtt gataggggtg ttactaatgg ttggcaataa
    11221 gtggtacaaa tgtgatcttc atattcattc aactgcgagt cagtgctttc aggataaaaa
    11281 ggttactgct aaggaatggg tagaagaatg tttaaacaaa ggactcgatt gtgttgcatt
    11341 aactgatcac aatacagggg aaaatattga tgaatataag aaagtggcta tcgaaaatgg
    11401 cttagatttt tttcctggag tggaattaac gtgtggagaa aatggaactc atttactagt
    11461 cctatttgat aaaagtgcat gtacaaatga tatctctgat ttcttaacca agatgggtat
    11521 aaaaagagag gattgggcca gcgctgcagc ttgctcaatg aaatcagttt tagaagttat
    11581 tgattttgct gagcacgagg gcttgctcgt tattcctgca catatagatg agtttaatgg
    11641 aatttcttca gcaaaatttg gaaatctagg aaagatattt gaatcagaaa atatatatgc
    11701 agtacagacg gttcaaaaag aattttttga aaatcgctca caaatgattc cctcatctaa
    11761 gcgtgacact atctatgata gtgtgaatgc cagatatgat ggtagagttg gaaaagatac
    11821 tttggaatct tggtataaat cagttgttga ggcagaaaaa aataatatga catttcttag
    11881 cttttctgat aacccacact cgaaaggaaa ttcaaaacat ggactttggg gaataggcac
    11941 aagatattcc tatataaaaa tgaaggacga acctgacttg ggatcacttc gcgatgcttt
    12001 aatgctaggc gaaactcgag ttcattctga tttttctaat ttctccatta atgaaaatga
    12061 agttttattg gaaaaattaa gcttttctgg gaccactctt agcacaaaag aagttgtagt
    12121 cgaattcagc gataatttaa cttcaattat tggaggaaga ggaacaggca aatcttgtat
    12181 tacaagattc ttggtctatg tccttggaaa ggaagcagaa cttgatcaat tttctgaaat
    12241 acaaagtgat tatcagaact ttgcacaaat tgaacataat ggctctggaa tatttttgaa
    12301 ggatacaatt gtgaaactga atattttcta taaggggaca aaatatcaaa taataagaac
    12361 acaggataga cattctattt atgaatttac acaagagaat ggtctggttg aaaaagaaac
    12421 agaacggttg agaatgattt ccgataaggt gagtatatac tctcaaaaac aaatttatga
    12481 aatttcaaaa aatcaaacta gtatactaga attactagat ggatataact ctgatttgat
    12541 ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa ataagaaaat taaattggga
    12601 tattgtttct gtgaaaaaag aaatacaaga taaggcgaag gtagagcttg aaattgaaga
    12661 tttaaaaaaa caagtagaaa agctatctca taagagttat aaagatgttt atgatgagta
    12721 ttcaaaagag acagatattt atagggaact gaaacgtgat gtggaggcac ttaaggaaat
    12781 tcccaaaaat ctaactgata gtgttgacaa aattgacttt ggcaatggta taaaagttac
    12841 agacgaaatt gatgaacacc gcggagaatt gattaagaat ttagtgacca atcgggctaa
    12901 gattcaaaca ataattaatg aaatgtcaga tgaaattgat agttatagag cagagctgaa
    12961 taaatcagat tggaaagtta actataatga ggtttcaaag aggtatcaaa gaggtatcaa
    13021 aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt tacaaaacct tgattcttta
    13081 tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa ttattcaaca agaaataaag
    13141 cttaaagatt accaagttga ggttgactca gcaatgtcaa gaatgaataa atcatatcaa
    13201 aatttctatt ctgagagaag tagttcctcg cgcgaaattt tttctgatat tacaggaata
    13261 gaggctacta ttcaaccaaa tagggatttc aataattatg ttaaagagct cagagcttgt
    13321 attaataagg aggacacttt tgacagtgag tttggatctc ttattgataa gctgacaaaa
    13381 aattccatta ctaatcaaga tatatataat gacatacttg atataagagt tagtggggaa
    13441 agtagtattt tttcagataa aaaattaatc aattctataa gaaaattgaa tgatgcacaa
    13501 gttcttgaat tacaaaccct taagccaaat gatttagtag ttatccgact tgaactaaat
    13561 ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa agacatctgc aattctttct
    13621 atgattttgg cgtatggaaa ttcaccactc atattggatc agcctgaaga tgatttggat
    13681 agtcaattaa ttaataactt aatcgtaaga agtatcatta caaaaaagga aaacagacaa
    13741 attttaattg taactcataa tgcaaatatc ccagttaatg gtgattcaga atggttgatt
    13801 agcatgggag attcaaaaga aatatcggct gatcagtgcg ggtcggtaga tgaacccaat
    13861 atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg atgcttttac aaatagagcg
    13921 aaacgctatg gttttaaaca aattgtagaa taacactaga gggtgagaca tattattatg
    13981 tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc tataccagta tctgggcagt
    14041 tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct tttcctgtga cttttaacca
    14101 aatggcttgg ttcgttttat ctttactgtt tgtcatcgta tttgcccatg tgcctccact
    14161 atctatgata gaggaggtct ttttaaaata cttaggcata ccggtggctg tcacttggtt
    14221 tatgtcgcaa aagacgtttg atggcaagaa accactggga tttttacgat cctttatcag
    14281 ctatcacttg cgatttaagg tgacttttgc aggaaagaaa gtcaaagaac agaagaaacg
    14341 atgggatgaa cccagtactc ttgtaaggag tgtgaactat gtatccgatt aagtatattg
    14401 aaaataatct cgtctttaac caagaaggcg agtgttttgc ctactatgaa ttagtgcctt
    14461 acaattactc ctttttgtca cccgaacaga aatttcaagt gcatgataac tttcgccaac
    14521 taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca aattgccaca gaaagtagta
    14581 ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg tagactcaaa gaagtagcga
    14641 aacaacgaat tgatatacag acagatgcat tagtatccat gattggcgat agtcagattg
    14701 attatcgctt ttttattggc tttaaactaa ttgcgacaga tgaagaagtc aatctaaaga
    14761 gtctcaagaa atctttcttt tctgggcttc aagaatttgt ctatggagtg aatcatcatt
    14821 tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ctgctattct aagcttgaaa
    14881 aattaatgga aagcaaattg gctcgaagat ttaaagtcag gcgagtcaca ccgagtgacc
    14941 tgacgtattt gattgaacat atttatggcg aaaaagggat accgtttgaa gaatacgaat
    15001 ttcagctacc aaagaaaaag ctaaaatcag aaacactggt gaaacgttat gacttattac
    15061 gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt acggatggaa catgagaatc
    15121 atgagtcgta cgtagcgtat ttaacaatta atacaattgt gggggagatg gaatttcctt
    15181 cctcagaact tttctattac cagcaacaac aatttacatt cccaatcgac acaagtatga
    15241 atgtagagat tgtgacgaat aaaaaggctt tagctaccgt tcgtaataag aaaaaagaac
    15301 tgaaagattt agacaatcat gcttaccaat ctgacaatga aacgaattcc aatgtcttag
    15361 atgctttgga ttccgtggat gaattggaaa ccaccttgga tcagtcaaaa gaatctatgt
    15421 acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt ggatgaacta aagcgacgct
    15481 gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa actagtccgg ccctttggcg
    15541 atatgatggg gctacatgaa gaattcttac ccttaagtaa acgatatatg aacgactata
    15601 ttcagtatgt tacgtctgat tttttggcgg ggcttggttt tggggctact cagatgctag
    15661 gagaattaga agggatttac tttggctaca atgtggatac aggtcgtaat gtttacctga
    15721 aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac caatgcgctg gccgccgctt
    15781 tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa tctcttagtc tactatgctg
    15841 ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg gggtgcgaaa cgttcctact
    15901 tgaaaagagt aggcacagca cttcattaaa tcaatattta atgtgtgttg gagaactaaa
    15961 cacacgaata gtcgaatgac ggggtaacgc cctgaaaggc tactttaata ctctgactgg
    16021 cagaaacttg agtaaacaag gctctgtcag aagctcggtg aagtcggcag acagacaccg
    16081 taacagataa tgctgacgaa agattcccag aggtgggcgt tgtgtcctat atgccggaag
    16141 tctataaaat atctatggtt agaatgtaga tgagaggtct actgacgaat ccttgaatgt
    16201 acaggtctat atcggggctg gatagaaatg tccagtgaca caaacagtgt cgctactgtg
    16261 ggtgagtaag actccgcatt tatgaaaatc catataacgt taaaggcgtt atcaaggtag
    16321 caggcttata gaggaaacct aaggatatat gtacagatag tgtgtttgga acgttgaaag
    16381 ctgacaacat ggagatttta ctgtctgtga agtgttcaca tcagaaagtt attgtgagat
    16441 tagcaatgat ataaggtgtg ttaatgtcag tgagagtggt ggcacagtac ctatgaaacc
    16501 gtgataacaa gcggtggagg gatagccact agtcatatga agaatagaat tactaagaaa
    16561 aatggtatgg gttcgagtaa gactaagaga tgtacttgct cctgcaaaca aggagggtac
    16621 agaccatgct aaagaaaaac aagctccgct ataacgagta ttatgatatg caacatattt
    16681 atgacgagtt atacgcacag agcaagaacg gtaataactt ttataagtta cttgaaatta
    16741 ttggttcgga acaaaatatt tgtttagctt accgtaatct gaaatcaaac agtggcagta
    16801 aaacggctgg aacggatgga atgacgatag acgatataaa acaactcagc aatgctgaga
    16861 ttgttgctac cgttcgggaa agccttagca actaccgccc caaatcagtc agacgtgttt
    16921 tcattccaaa agctgggagc gataaaatgc gtccattggg gattccgtgt atctgggaca
    16981 ggctggtaca acaatgtatc ctgcaagtcc tagagccgat atgcgaacca aagttccata
    17041 atcattccta tggattccgt gcgaatcgaa gtgcccacca tgcagtcagc agagtaacga
    17101 cactgataaa tttatcaaaa tatcattact gtgttgacgt agacattaaa ggatttttcg
    17161 ataatgtcaa tcatggaaaa ctattaaaac aaatctggac acttggtatt cgggataaaa
    17221 gattgatttg cattatcagc aaaatgctga aagccgaaat tgacggcgag ggtgtcccag
    17281 aaaaaggaac gccacaaggc ggtctgttaa gtccactgct ctccctcata gtccttaatg
    17341 aattagactg gtgggtaagc agtcagtggg aaacattcca gccgaagaac cgcagtaaaa
    17401 atgggtggct tcaatacgca aagaaatata ccaaactgaa aagcggtttt attgtacgtt
    17461 acgctgatga ttttaaaatc atgtgttcaa cctatggaga agcacaaaga ttttaccaca
    17521 gtaccgtaga ttttcttaat aaaaggctga aattagaaat cagtccagag aaatcaaagg
    17581 tagtaaatct gaagaaaaat tcatcagatt ttctggggtt caaaatcaaa gtcattccaa
    17641 aaggaagaac gaaacacggt tatgttgcca aaacagatat gaaccaaaaa gcattaaaga
    17701 aagcgaaaac aaatctaaag ttaaaggtaa aagatatagc cagacatacg actggcttca
    17761 atatcagcag atataatctg acagttatcg gtatgcagaa ttactactgc atagcaacta
    17821 acgtatataa caacctgaca gaagtaagtt acgctctctt accgaccata cgaatccgtt
    17881 taaggaacat tgcaaaatca gttccatttg aaagtacaag ttcagaattt cagagcagga
    17941 caaaaggaat cagaccaaaa acaaaaatag tgatgattgc ggataatccg ctattgccga
    18001 ttcaaggagt acaacacaaa aatcctatga atttctcgca agatatttgt aattttacaa
    18061 agcaagggag aaacaaggtg catgaggacg ttgtagttgt aacaaaagaa gaaatccgag
    18121 cgttgttgga aaatgaaaac cctgccgata gcgtagagtt taacgacaat cgtatttcgg
    18181 catatatcgc acaacaaggc aactgctatg taatgaatcg tcgaggaact ccgtccactc
    18241 tgatatgtat tcacaaatca gacaaaggag ataaccttga cagatattct aatctggcat
    18301 ttgtggaaat tcccattgca aaggcaatcc taacagaatc agcagacgaa gcaaaatcac
    18361 tgttaaaagg ttatgttctt aatagtcagc agaagaaaaa actgaacaga atacggacga
    18421 attacggata ccagacaata actgggaaat aacttataaa tctatttgga tatgatggaa
    18481 cgccgtatga agcgaaagtt tcatgtacgg tgtgaagcgg gggaaaactt ggagataaca
    18541 tcaaaaagtt acctatcgct atagcgagga gggtggaaag aaaccttgcc agaaattgcg
    18601 gatgaaatca gtatcctcaa cttgacgagt aagtcagaaa atcaaggtct gcttgatcct
    18661 tatgtgatta tgaagaaaaa gaaagactcg gaaagtttag caattgatat tctgactttt
    18721 ttaacaggaa tttcaagtcg agacggggag aagtttcctg tgttacgtaa agccattcga
    18781 acggtgactc aaagtgatca acgggggtta ttacaagtta ttaacgaatt gcgaaaagac
    18841 gggtcaccaa tcgcggaaaa tatcgccgat catatcgagt cctttaccga ttatgatttt
    18901 gctcatttgc ttttctcaga cggctcgatt acccaatcga ttagtttaga taaacagcta
    18961 aatatcattc aagtggcgga tttggtttta ccggatgcag aaacgacttt tgaagaatat
    19021 accaccatgg aattgcttag tgtttcgatg ctaatcgtca tttccacttt tgccttggac
    19081 tttatccata gtaaccgaga aatcttcaaa attgtcgatt tggatgaggc ttggagtttt
    19141 cttcaagtgg ctcaaggaaa aacgttatcg aataagcttg ttcgtgctgg tcgggccatg
    19201 aatgctgggg tctatttcgt cactcaaaat agcgacgatt tattagatga acgtttaaaa
    19261 aataatatcg ggttgaagtt tgcttttcgc agtaccgata ttcatgaaat caagaaaacc
    19321 ttggaattct ttggtttgga tcaggaggat caaagcaatc aaaaacgcct gcgagatttg
    19381 gaaaacg
//
LOCUS       DAZKOM010000049        19155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000049 DAZKOM010000000
VERSION     DAZKOM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.049"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1499
                     /locus_tag="ACG8NF_001660"
     CDS             72..1499
                     /locus_tag="ACG8NF_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472144.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1591..1956)
                     /locus_tag="ACG8NF_001661"
     CDS             complement(1591..1956)
                     /locus_tag="ACG8NF_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0472145.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1953..2318)
                     /locus_tag="ACG8NF_001662"
     CDS             complement(1953..2318)
                     /locus_tag="ACG8NF_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0472146.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2510..2758)
                     /locus_tag="ACG8NF_001663"
     CDS             complement(2510..2758)
                     /locus_tag="ACG8NF_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472147.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2757..2885
                     /locus_tag="ACG8NF_001664"
     CDS             2757..2885
                     /locus_tag="ACG8NF_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0472148.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2942..4636
                     /locus_tag="ACG8NF_001665"
     CDS             2942..4636
                     /locus_tag="ACG8NF_001665"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0472149.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4736..4909
                     /locus_tag="ACG8NF_001666"
     CDS             4736..4909
                     /locus_tag="ACG8NF_001666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472150.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5287..6420
                     /locus_tag="ACG8NF_001667"
     CDS             5287..6420
                     /locus_tag="ACG8NF_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0472151.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6451..6771
                     /locus_tag="ACG8NF_001668"
     CDS             6451..6771
                     /locus_tag="ACG8NF_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0472152.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6800..7264)
                     /locus_tag="ACG8NF_001669"
     CDS             complement(6800..7264)
                     /locus_tag="ACG8NF_001669"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472153.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7396..8649)
                     /locus_tag="ACG8NF_001670"
     CDS             complement(7396..8649)
                     /locus_tag="ACG8NF_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0472154.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8678..8995)
                     /locus_tag="ACG8NF_001671"
     CDS             complement(8678..8995)
                     /locus_tag="ACG8NF_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0472155.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9021..9341)
                     /locus_tag="ACG8NF_001672"
     CDS             complement(9021..9341)
                     /locus_tag="ACG8NF_001672"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0472156.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9805..11220)
                     /locus_tag="ACG8NF_001673"
     CDS             complement(9805..11220)
                     /locus_tag="ACG8NF_001673"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0472157.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11237..12553)
                     /locus_tag="ACG8NF_001674"
     CDS             complement(11237..12553)
                     /locus_tag="ACG8NF_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0472158.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12703..13401)
                     /locus_tag="ACG8NF_001675"
     CDS             complement(12703..13401)
                     /locus_tag="ACG8NF_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0472159.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13690..14679)
                     /locus_tag="ACG8NF_001676"
     CDS             complement(13690..14679)
                     /locus_tag="ACG8NF_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0472160.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="ACG8NF_001677"
     CDS             complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="ACG8NF_001677"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0472161.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15767..17401)
                     /locus_tag="ACG8NF_001678"
     CDS             complement(15767..17401)
                     /locus_tag="ACG8NF_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0472162.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17388..>19155)
                     /locus_tag="ACG8NF_001679"
     CDS             complement(17388..>19155)
                     /locus_tag="ACG8NF_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472163.1"
                     /translation="NLTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLST
                     LYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTI
                     IPAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATY
                     RTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLL
                     CSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIH
                     SPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYV
                     GVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYS
                     WLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTE
                     AIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVN
                     GKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFA
                     PNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6080 a         3748 c         3187 g         6140 t
ORIGIN      
        1 tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg
       61 gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag ctgtttttat
      121 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
      181 tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga
      241 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      301 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      361 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      421 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      481 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      541 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      601 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      661 aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      721 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      781 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      841 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      901 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      961 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc
     1021 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt
     1081 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1141 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1201 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1261 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1321 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1381 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1441 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1501 gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1561 aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1621 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1681 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1741 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1801 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1861 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa
     1921 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1981 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     2041 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     2101 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2161 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2221 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2281 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2341 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2401 tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat
     2461 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2521 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2581 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2641 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2701 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2761 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2821 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2881 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2941 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     3001 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     3061 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3121 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3181 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3241 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3301 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3361 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3421 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3481 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3541 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3601 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3661 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3721 agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata
     3781 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3841 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3901 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3961 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     4021 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     4081 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca
     4141 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4201 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4261 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4321 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4381 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4441 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4501 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc
     4561 ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4621 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4681 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4741 agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4801 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4861 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     4921 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4981 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     5041 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5101 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5161 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5221 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5281 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5341 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5401 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5461 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5521 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5581 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5641 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5701 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5761 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5821 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5881 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5941 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     6001 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6061 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6121 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6181 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6241 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6301 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6361 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6421 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6481 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6541 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6601 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6661 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6721 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6781 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6841 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6901 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6961 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     7021 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7081 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7141 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7201 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7261 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7321 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct
     7381 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7441 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7501 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7561 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7621 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7681 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7741 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7801 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     7861 gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     7921 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     7981 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     8041 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8101 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8161 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8221 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8281 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8341 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8401 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8461 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8521 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8581 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8641 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8701 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8761 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     8821 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     8881 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     8941 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     9001 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9061 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9121 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9181 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9241 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9301 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9361 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9421 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9481 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9541 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9601 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9661 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9721 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9781 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
     9841 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
     9901 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
     9961 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
    10021 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10081 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10141 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10201 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10261 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10321 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10381 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10441 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10501 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10561 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10621 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10681 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10741 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10801 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    10861 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    10921 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    10981 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    11041 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11101 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11161 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11221 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11281 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11341 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11401 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11461 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11521 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11581 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11641 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11701 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11761 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    11821 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    11881 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    11941 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    12001 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12061 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12121 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12181 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12241 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12301 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12361 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12421 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12481 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12541 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12601 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12661 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12721 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12781 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    12841 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    12901 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    12961 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    13021 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13081 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13141 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13201 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13261 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13321 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13381 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13441 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13501 catagacata caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc
    13561 tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc
    13621 ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt
    13681 acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa
    13741 attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac
    13801 acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc
    13861 tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc
    13921 ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag
    13981 ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc
    14041 ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg
    14101 catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt
    14161 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    14221 catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    14281 gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga
    14341 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    14401 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    14461 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    14521 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    14581 ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata
    14641 ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat
    14701 ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt
    14761 aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca
    14821 gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt
    14881 gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg
    14941 gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt
    15001 gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc
    15061 caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta
    15121 tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta
    15181 cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga
    15241 tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg
    15301 cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct
    15361 tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc
    15421 ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat
    15481 tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg
    15541 gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc
    15601 ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa
    15661 taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa tccttatcga
    15721 cagattctcc tttttaatca atttatttta taaatgaaaa gttaatttac cttttatgga
    15781 aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa gaaataatag
    15841 tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact ccgcttattt
    15901 gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt tctacattcg
    15961 ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta gttactctat
    16021 atatgataga actaccagtt gttttataag gaaggtttac ccattgactg ccaatctttg
    16081 cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa taatcatata
    16141 aaaaataatc cattccttta gtggtatacg cctctactgt ttctttgttt acaacaatat
    16201 gtcccggctg agaaattatt ttttgattga aatttttttt actagaaaca gttaaagtga
    16261 ttaataacaa aacagtcata aataaataaa atagttctct tgtcttattg tatcttctat
    16321 ttttgaaaaa tatcatcgtt aatatcagag aagagaatat tatttgcaag cttaagatcc
    16381 gccaaggaaa ttgtaacaat ttggctggcg tattttgcag aagataccat ggcaccaaac
    16441 tagttgtagc tagccaagtc aaacacgtac cataataaat ctttttttct atgtttgtta
    16501 attttttaaa taaaatcagg ggaatacata aagataacag accaactagt cccattgagt
    16561 aagtagttaa gtcgttgttc atagcttcag tcagactatc gaacacatta gatgcatgag
    16621 catataaatt tgtcacgcta ggtttgttga ttttttggta gagcatttgt tcaaacatcg
    16681 gataccaaaa agcactcgtc agaaccaatg tcattcctgc cgcactaaat aattttttaa
    16741 atatagcaat aaaatctgca ttcttgaaac ctttagaaac caaacgaata aaaataaaaa
    16801 tgataattaa tgctgtactc attaatgttg atagtacatg gctgtacact agcaaactca
    16861 tactcaatgc tagctttaca gacggatact tttcttcttt tccagagatg atttgataag
    16921 cataaaataa tacagggata acaagtgtta ttgcgatagc ttcggcaatt gcggaacgat
    16981 agtagatgtc tactgtccga taagcagaga aaatatagag acaactaaat aaaaatgccg
    17041 cttttttttg ttttaaaaac cgctcgccat aatgataagc tacgagtgtt gtacacaagt
    17101 tcaataacca aacgtaaata atatagctta caatcaaatt ttttgtcatc caatataaca
    17161 ttacagctgg aaaaaaagtc agaaagggat aaaaataatt tacaccatat ccagtataat
    17221 tgaatgtctc ataattaatc ggactggtaa aaatagtcga taaccccttg attcgagata
    17281 agtgaaaaga aagatctcca gtggtataaa tactctgtgt atttttgaaa aagatgaaag
    17341 aatatatact actaactata attaatccac tcaatattac tactttttta tttattttca
    17401 ttcttcttgt ttatctcctt tttagtcaca tatcgagaat ctttcaactt attaggtgca
    17461 aatcgtttaa aacttgaata caccactaaa caataagtca gaatactgat ccataaagcc
    17521 atgagagcag ttggagtata ttttgttgta atttctatct tattttctcc agcatttaca
    17581 tccacattca ctaagccatg ttttttataa gagtcattta cgactttacc gtttactttt
    17641 acctgtgtac ctaaataagc atatactggt actgtcactt tttgcttttt gttgcttgcc
    17701 actgtgtaag taatgttatt atctgcagct ttatacttcc ctttttccca cgattctcct
    17761 acttttactt catgtttttc taagtgaggc ctttctttta tcgcttctgt tggggcgtaa
    17821 tcgtataatc ctcccatatc tgatgttgta taacgtggga gtgtttcttc attgataaca
    17881 atatgaccgt gttttgattg gataactttt gcgtaatttt cttttgctgc tatagacgtg
    17941 acgagtagaa acaccacagt agcgccaatg attaaccatg aatatttttt attcatagtc
    18001 tttttcataa aaacaagcgt tatgatcatt gaagaaaaaa agatttggat acctaagata
    18061 cgccaaggaa actggagtaa gcttatcggt gttttttgaa acaaaaacca tggaaataaa
    18121 ttagttacgc atacccatgt agaaactacg ccaacataga tgattttttc ttttttctcc
    18181 atttctttca gtatgaataa aggaaaaatc aacgcaacca atcctaataa ccccatagtg
    18241 tatgttgtaa tatcgttatt taacgcttga atgatactat ctgaaataga aagtgcttct
    18301 agctgcaaat ctgttttgaa gggactgtgt atttcttgat aattcatctg ctgcaacata
    18361 ggataccaaa aatagctagt taatgcacaa gtcaaaaaaa cagcctttac taaattcacg
    18421 accacaaatt ttatatcgtt ttttatttgc ttgcttaaaa acaaacgaac tataatcata
    18481 caagcaatca atgcactaca caatagtgta gttaagacat gagtatacac aagtagactt
    18541 atactgacag ataaagcgat gacttttttt tctctattga atatcaatcc ataagcataa
    18601 tataaaacag ctgggataat cgtaagagcg attgcttctg caattgcgga tcgataatag
    18661 atatcaactg ttcgataagt tgcaaaagta tataaacaac taaataaaaa agcagcatct
    18721 atctttttaa aaaacttttc tccataataa aaactaatca gaatcgtaca aatatttaat
    18781 aaccaaatat atagaacata acttaatatt aagttatttg aaattccata aaaaacgact
    18841 gcaggaatta ttgtaagaaa aggataaaaa tagttcagtc catctccata attgttaaat
    18901 gttgtatagt tgattggccc ttcaaaaata gaagataacc ctttgattcg tgaaagatga
    18961 aaagataaat cacctgttgt ataaatttct tgagcatttc tcataaaatt tactgaatat
    19021 aaagtactaa gtaagataag aactactaaa agaaatgctt ttttacttat tttcatagat
    19081 ttgttgttct ccttttttaa ttctattttc agtatactag aattgtcttt ttttaataag
    19141 ttttgcgtta aattt
//
LOCUS       DAZKOM010000050        18628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000050 DAZKOM010000000
VERSION     DAZKOM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.050"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..925)
                     /locus_tag="ACG8NF_001680"
     CDS             complement(14..925)
                     /locus_tag="ACG8NF_001680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0472164.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1302..1940)
                     /locus_tag="ACG8NF_001681"
     CDS             complement(1302..1940)
                     /locus_tag="ACG8NF_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472165.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1944..3926)
                     /locus_tag="ACG8NF_001682"
                     /pseudo
     CDS             complement(1944..3926)
                     /locus_tag="ACG8NF_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4106..4771
                     /locus_tag="ACG8NF_001683"
     CDS             4106..4771
                     /locus_tag="ACG8NF_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472166.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5168..7201)
                     /gene="brxL"
                     /locus_tag="ACG8NF_001684"
     CDS             complement(5168..7201)
                     /gene="brxL"
                     /locus_tag="ACG8NF_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0472167.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7319..9841)
                     /gene="pglZ"
                     /locus_tag="ACG8NF_001685"
     CDS             complement(7319..9841)
                     /gene="pglZ"
                     /locus_tag="ACG8NF_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0472168.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9957..13589)
                     /gene="pglX"
                     /locus_tag="ACG8NF_001686"
     CDS             complement(9957..13589)
                     /gene="pglX"
                     /locus_tag="ACG8NF_001686"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0472169.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13653..17324)
                     /gene="brxC"
                     /locus_tag="ACG8NF_001687"
     CDS             complement(13653..17324)
                     /gene="brxC"
                     /locus_tag="ACG8NF_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0472170.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17349..17924)
                     /locus_tag="ACG8NF_001688"
     CDS             complement(17349..17924)
                     /locus_tag="ACG8NF_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0472171.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17924..18523)
                     /locus_tag="ACG8NF_001689"
     CDS             complement(17924..18523)
                     /locus_tag="ACG8NF_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0472172.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5739 a         3561 c         2609 g         6719 t
ORIGIN      
        1 ttatttatac cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa
       61 cattttcaaa gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta
      121 ctttattctg gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt
      181 ttttgtattt atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat
      241 attccgtagt ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt
      301 tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt
      361 tcacttttcc gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag
      421 agaaatcgtc tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta
      481 cttggataat ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt
      541 catcggtcaa tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac
      601 tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac
      661 ccaattgttt ctttgtttga tcatattggg aatcgatttc tttggttgat acgtcatctc
      721 catatttatc ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt
      781 gcattttttt caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa
      841 cggctgtttt ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg
      901 caagtgctaa aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt
      961 gctaaaaaca aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga
     1021 tacgtcctga atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt
     1081 caagtagcac atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct
     1141 tgcgttccgt atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt
     1201 cttccgctgt tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt
     1261 tgttgttttt attagaaaaa gtttgttttt tcatttgttt tctaaagcat gatcttcttg
     1321 tttcctattt tggtcagata tggatcatgc gtgaccacga taactaacgt accttgggaa
     1381 gcctgttgcg ataagatttc aacgacttgc tccttatttc gtttatctaa tgatccagtt
     1441 ggttcatcgg ctaaaatgat tttgctattc ttcaataaga ctctcgcaca ggcgattctt
     1501 tgcttctcac caccagataa tgtgtagata ggcgtactta gcttcttgtt tatatttact
     1561 tttttcaatg cctcatactt tgctgttcgc ttatctgaaa catttaaatt cgaaaacttc
     1621 aatccaatat ctaaattaaa atcgattgtt tcatattcaa tcaacccaaa atcttgaaaa
     1681 agaaaagaga gggcatttct ttttatcaag cgcttctctc ttccagttaa atgttttgtt
     1741 tcttgattaa ataaataaaa ttgccctgaa ctgaattgat cattcaatcc taatatattc
     1801 aataaagtac ttttccctgc tccgctctct ccaactacag tcacaaaatc cccagcatcc
     1861 gcagtgaaac gatccaaatg aagaatcgtt ttgtttcgaa tgattttttt cgcacctttt
     1921 atcactatca acggctccaa ttgttaacct ccattcatca gcaataactg acttttcttt
     1981 tctgtagaca aaataaaaac aactgaaaga ataaaatcaa tgaagctaaa gaaaaaaata
     2041 ttaaaaagat atttaggctc tctttctata agaaaaagga agattacact tgtcgttccc
     2101 catttgatga ggttcatcat gaagaaatct ttatacgttt caaagagcga ataaccactt
     2161 gaacgaaaga tagcgatttt ccgacaattg tagacaaaat atatgcataa catgacatag
     2221 ctcaacaaga agaaagaaaa gaaagtgagt aaaaatatct gtatcaagta atgaaatgat
     2281 ctgcttaaca ctttgacagc tatctcttca gtctgagtat acggaaatga taactgcaag
     2341 ttatcacgta actgactttc ttcaatcagc tcaaataacc tttctttcga caaagagcct
     2401 atcttgatct ttaacggtgg atagatttct ccgctgagaa tatttctttc ccaatacgta
     2461 ctgtttttca atgtcagtat ttcaacaacc ggataatgtt cgatccaggg gatagaagga
     2521 ataaatgtat aaatgggttg attgtttttt ataaatagaa atttcggttc tttttcactg
     2581 atttcttgat aatattctgt tatttgcgcc aggtcatttt ttaaatctgt atgtctatat
     2641 cgttcaggga tcaatataac agggtgcgtt tctttttcgg ttatttggat caaccttcca
     2701 gagacatcaa tcaaaggaaa tttcttaaga taattcagat ttatttttat ccctctgcca
     2761 aactgttcat tttcttcatt caagtaatct gatacattga ctaatatact tccgttttta
     2821 ttcaaagttt catataattc ctgatcggct gctgatgcaa aacgattgga ataaataaaa
     2881 tccacgtggt ttttcccgat ggttactgga taaaatacat gatagtcctc accagcgatt
     2941 tcttttggtg tttcatggga aacagctaac tgtgaagaag aaaaaacgat agttgctaat
     3001 tcgacattgg caacggtcaa aagaagagaa aacaacaatc caaatgtaaa aagaatgtat
     3061 tggatatagt tgaacgcttc tcttaacgaa gcgatttttt caactaataa aagagaaatc
     3121 aaagagatac cctatattcc gctgaacaag aaaacgtaca tctggaacaa ataaagaaat
     3181 tgctccttat aatacataaa aataaaacca ccaatgattc ctgcaattat gaatgtaata
     3241 atgattttct ttttccccaa taaacaccac aatacttgat gtaatgaaaa gccattattc
     3301 aacaataaac tgatttcttt attcatcaat aagaaaaagg atgataacaa aaggaataaa
     3361 agaatcaatc caatagttaa atacactcga taattatata cgtctgtact tgaaataacc
     3421 gaatacgaat agctattttc aaagtcttca aaaatattag tggcataatc agtgtgaaat
     3481 gcttgattga atcgttccac aaatagtaaa atgaaccgtt cataggcttc tttttctctc
     3541 gtcatcaaga aaaattcacc ttcaaaatct tttattttga tcgattcttc aatggacgtt
     3601 acgcgaaagg aggtattaga atagggtacc ttctcgtgag aagatttgac atccccttga
     3661 taatacaatg tcatttcaag ctttggctgt atcttgaagt tccaagatgt atctacttca
     3721 ttggattcat ttactgtttt tttcatataa gaaagctcag tttctgatga gacattttca
     3781 aataccatga ttagctgctc atattcttct tgcccaataa aatgttgtgg caatcgaatc
     3841 ggagtataag tatctgtttg atggaaaaat tcatttgtcc gatagagtac ctgtttattt
     3901 ataataacca aggcaaaacc actcatgata ccggtcacta tgattgtgag taaaactgtg
     3961 aacttttttt tgattatcac cactctccct tacttaaatg attctttgat aaaatcatac
     4021 cgtttttcat caaaaaaacg tatctatcaa atgtataggt catgtagaat aaatctatat
     4081 actgtagaat cgaaaggaga agaagatgaa catcggagaa aaaatacgaa cttaccgcag
     4141 aaattgtaag cttacgcaaa aagaattagg cgaaaaaatc ggtgtttccg ataaaacaat
     4201 ctctagttgg gaaaattctc gtacaatgcc agaccttgaa atgctgtcac ttttacatca
     4261 gactttaggt ctgccgattg attttccttc tattgaatca gctaccgaat caatagaacc
     4321 aacattgccg gatgaacctc ttgttatcaa gaaatcttat tcatcaaaac aacccttctc
     4381 actcaaaaag acactatctt tctttctgct cgtcttttgt ttggtctatc tttttcttat
     4441 caattatcaa tataaaaatg aaacggttga tcgattcaat ccttttttga aaccacaaat
     4501 cggctatacc acactccctg taggatacgt ggaaaattcc atcgaacatt gggtatgtaa
     4561 tgatatcttt gttcatggta catggctgtc ttttcacgct ggagaggtag aaaaaggtaa
     4621 gaatcatgct gtaatcaaac acaaaggaaa acacgtcatg aacgttcgcc tgctcacttg
     4681 ggaagccatt ccctcagatt accaagaaat cctcatccaa gactatgacg atgccttaaa
     4741 aagaagccct caggagccag ataggcatta agtaggagat gaacgtagag atagaaaaaa
     4801 tcaatgcaca tgatcttatc aaaaaaaaac aggggtacaa attaattgaa aaagctttga
     4861 gatgaaagtt cctaaataaa aaagttcaga atgataggta aaaaaatgac taactattct
     4921 agtattaggc tatttgtatt attcttctgt tatttttata tcaaaaaatc cagaaataac
     4981 taaatatagg tatttctggc ttatagtata atatttacgc cacagccccc aatgattctt
     5041 tttggaatca tgatcaagga cggacataca caaaaatatt ttgcccattc gatccgaata
     5101 gattaacgac taatctagta agtaatatag attggtacgt agtcaaacca gcaaatggaa
     5161 aagttgtcta ttccacccca agggctttaa agacagcatc ttccggcgtt tgataaaaaa
     5221 tcaaattaaa ggtacccatt aattctgggg gtacagttcc aatatcagca gcgcttatca
     5281 taggtacaag tacttttttc gctccggcat cttttgctac ttgtaaacag ttagctaaat
     5341 tatctacctt aatcatagaa ccaccaattg taatatcccc taaaataact aaacttggta
     5401 atacaggttt atttaaggaa actgagcata aagctattat agttgataac gataatgttt
     5461 ttgtcatacc aatgccattc atatcttgaa catgaacgac aaaatctttt gtcgtcaaac
     5521 taatagactg actaacactt ccataattag ctcttaaata tcggtatgcc gaatccatcg
     5581 cttctcttgc tccagcatct gaattgatcc cagtacgttc aaactttcca tttcccgaag
     5641 tcatttgcgt ttccaattta taggtaccta attgcccagt tttgccataa gataccgtgt
     5701 acaaatgccc tggcttccct aacccttctg gaatcaaagt tcctccacct tgttcaggaa
     5761 cagaaacata ctcttctcgc tcttcttcta aatcaatgta tgaaaaattg acatcataaa
     5821 attccattcc accaattttc tttaattgtt cttttacacg acgacgcatt tcaagagcaa
     5881 atcttagtat ttcttcgaca tcactttttt caaatctacc atcaggatac aacagtttca
     5941 tcatcccaga tacaatccga cgaactgcaa tcgtatcccg ctgatttaat tgagaaccaa
     6001 gtttgaaata tttatcaaaa ctatcgccaa aactaacttt tcgaagctca cgcatgactt
     6061 cagaaaaata atctgtaatg aagccatagt catttgtaaa aaaatctggt cgatatttag
     6121 gaatttccca acctggtaaa taacaatgca tccgatccaa aaaggctgaa tccgtcccca
     6181 taacctccgg aaatggctca aataaattcg aagttttcaa tattacatca acgctttgat
     6241 taatatttcc tacaaaagcc attgatgctg aagcattctt ttcttctttg cctctagcaa
     6301 aagatcctga agccatgtaa tctttcatga tttgaacgcc atcattatcc ttaaatttaa
     6361 tcccagcaac ttcgtcaaaa gcaacacagt cccacatacc tacaagtccc attgtacgac
     6421 tagccatatt atagaaaagg tttgctactg ttgtttgtcc acctgaaaca aggattgagt
     6481 ttggtgaaat ttctttatat acatgcgatt tacctgtacc acgtggtcct agttcacaga
     6541 gattaaaatt attttcaatt aatggaataa gtcttgctaa atgaagccat tttacccttt
     6601 tattaaattg tgtaggttcc ataccaatgg accgtagaag taagtcaatc cattcatttg
     6661 tagtaaactc cttgcgagcc atcttgtaag cctcaatgtc taaaccaggc atttggattg
     6721 gatcaacctt ttttgcaata aaagggctat tccaacgatc attctcatca taccaatatt
     6781 ccatcgaaac aatacaccag atccccccac ctaataatcg atcaaatttt ttcggatact
     6841 cttcactaat ttgaacattt tttaatccca ggttagagaa ttctgcttca taacaatctt
     6901 tcttttcatt taaatgtaca gttactttat caataactgt ataaccaccc aattcacgaa
     6961 tttttgcttt tactttttct gcttcatcag gacgcacata gtttcgagca agaatatttt
     7021 taacattttc cacacctttt tcaaccaatg tttcatccaa tgacgaagca tacataccaa
     7081 gtaaatattc caatacataa acaggtacat tcgcaccttc tttaattttc tttgttaaat
     7141 cttttctgac aatctttccg ggaaagaatt cagataactt taaaatttcc tgttgttcca
     7201 ctagaattcc tccatgtgtt cagtcattat aatagcaatt aaagtatatc atacttaacc
     7261 agtaggcttg ataatacaaa agaaaaagta tccacaaaaa attgtggata cctcgttttt
     7321 atatatcaaa atcaaatgat tctgcaaaag gattatctat aatgaatttt attcgctcgg
     7381 tcaaatcatt tttttgattt tttatcacta aataataagt atcttcaact gtatatcgac
     7441 gatttacaaa ttcaaaaaca aatttagtaa accgctgatt tgcatcttct gtttttgaat
     7501 ccgcaacata gatttcttca tttgagatac gttcaccata ttcatcttca aaataaagcc
     7561 agaaagatgc ggggtcataa acatcactaa ttgcattttt ttgatagaat tctaacgtgg
     7621 ttgataatcc tgtaattctg cggttgctag tcattaattc aattgctact ggttcctttg
     7681 cattgctacc tttggctacc gtaatttcta gaacagggac tagcatttct tgaatagatg
     7741 accctccatg aacgtagctt tgtcccccgc cagctttctt aaaaatactt gaagtaatcg
     7801 ggacacaaat gtttctagtg tcatcatttc ctaaagaatc tcccaaagag taatttttcg
     7861 taccaattat ttcgtattct tcttcactaa gaataaaacg acgttctaca cgatcttttt
     7921 catttctaga tggattttca atcttatcta cttcttcaac gtggcttctt ttataaataa
     7981 atccatgatc agccgtaact ataaaacgat agatactgcc atcattatgc aagcgattaa
     8041 taagtcggga aatttcgctt atcgctaaat cacaggcctc aaaaactcga ttttcattcg
     8101 agtgatcacc tgttctatcc acttgatcgt gataaatata aatgactgat ttgccattaa
     8161 atacttctcg tacctcagcc ttattcatct taaccacatc atcataattt aaagctacag
     8221 catttgcatc tcgttgctgg agaatcgcat ttcgattgga taaaccattc gtttttttcc
     8281 catcaatcag tacttctgtt ccttcattgt aagttagttg attgtttggt aataaggcgg
     8341 ctttaccgaa ttcagtaact gaaggtaata cagaaagaaa gctagccatg tttgttgaat
     8401 acttcttttc tctaactaac ttacgttcta aattcatccc taactcatat cgaaaagcat
     8461 ctgaaataat aactacagtt ttaacagagc gtttcattac agcttgatta taaaattttc
     8521 gaatggatgg acgattctta aattcaaaag tttgattcca aatttgtcct gattgatcaa
     8581 gaaacttcga ataatttgct tcaactaatc gatacaattg gccatatttt tgcttttctt
     8641 ctatatgatc actattccaa ataaatttcc gataatgcgt atcaacccaa tacatttcct
     8701 tctcatattg agttagcaaa ttatccatgt tcatcggcaa gcacaatctg tttttatcca
     8761 caagataata agcatgtttc aataattgat actcataaat ataatccgat gcgaaatgca
     8821 ttttttcgcg ctgctttaaa ctatcaaaaa ttgtttgtcc tgcaattttc gttgtagtgt
     8881 cagaagcctt taatcgttct gcataaaaca ttaatatttt tgcatgaatc tcagaaaata
     8941 cgtctgcttc aattagatca tcaatttcta tactgtctaa taatccatca ccatcaatag
     9001 cctgatatac ttttttagat agctgttcaa agctttccat gtaacgtgta tcattcataa
     9061 actgatccat gaaagtaata atgtttgctt tcaaatgtag tatttcaaat cctttgaact
     9121 tcttcggcat ctcttgatca atttgataat aaaaatagtt caaataaaga cacatcgtca
     9181 gcttttccaa tgttggctgg ctatccgtaa agccaaattt catttgaata aattcccaaa
     9241 atttagctgt taactgatac ttatcaagtt cccttaaagg attctcccta ctttctgtaa
     9301 agctttttag aactacacgc agtaaatcat ttaatcgaat acttttcgat ttcaacaaaa
     9361 cagccattat cattaattca atcgtttctg atttattaat tgtcatatgc gtactaaatt
     9421 caccaaaact tctcatgcga tcctttgcat taaagaaatt acgatactgt ttaactgtct
     9481 gatgaaatct aagtggaata gccaattcat tcatgataat cgctaagcgg tctgcactaa
     9541 attcctctga ataattcaat acactcaaca aatgattatc ttcatcttta tcatcccaac
     9601 gaggaaatgg cgcatagatt aaatagttag tttcgggatc ttcatattcc aataaacgct
     9661 tagttttaaa ttgtgcgtcc ttttctaatc gatagatttt tgcattttga atgaccaatg
     9721 agtcaatttc ttcaagaaaa tcctgatttg aatcaaacca aaaaatgatt ttcctacatc
     9781 cataattagg aaaacgttga tttaattttt cttcaatttg ttgcgtattt atatctgtca
     9841 tcaatctcac ctaatcgttc tctttctata ccccttatat tgacataatt tggataattt
     9901 cgcacgcaat ttaagaataa aaaagaaagt tgttagaaaa caactttctt agataactat
     9961 ttttttggtt ctttgacccc ttttgcaaaa atttggaatt tctcgccggt tttaggatca
    10021 cgctgcaact tatcataatt gacaattaca ccatcatcta agtctaattc aattcgtttt
    10081 aatgctaagt gacccaactt ctcgtcatac tcacgaactt cttttaattg cttctgaatc
    10141 tttacaattt cttttgtcat tgcatttctt tcttttgggt tatcacttgt tgaaatttgt
    10201 tgctgacgca attcgattcg attgtcatac gctttttgta gttcgtgtag gtattcagta
    10261 cgtacttttc ctaattgatc tggtgtatag cgatgcaagt acattaacgc tttaaagcca
    10321 ttttgttttc ctgagtccaa ttgccaataa attggtcgat tggagtatgt ttttaaatgg
    10381 tctttgtaaa aatcttgtac gaagtaacga cgaataattt ctcgacttgt ccctttgcca
    10441 cctaaagtgt cagcgataaa ctggagattt tcttcaaggg tttctttacc aaaagtaatc
    10501 tcaacgaaat caatgaaacg attgacaatg tcaatttggc tatccttgaa atactcatca
    10561 tcggtgatga gtaaaacatt atcagtatca ggtttaaatt tttgatagcg ggaaggatcc
    10621 cattcgccgc cagcgaagat gagaccatct tcgtctaggg agtaacgacc gaagatacaa
    10681 cctattgaat aagaaataaa atccttaatt tccctcgcct tatcaggtaa ccttgcagag
    10741 atatattcat cttgaacctc ttttgttaat tctttttcaa gagaataatg attaatgaat
    10801 atgtcgttca gttcctcttc attcgatttt agagtattaa attgattaac caactgccta
    10861 cttagacagt taaatgcctc tgagagatct tttttaaaca tataacttaa caaaatgttt
    10921 cttctaaact cgtatgacgt ttcaagagca ttatagtctt ttctagctat atcgatattt
    10981 tcatccactt tttctcttat tttttttaat tttctctcag atgtaacttt aagaaaaggt
    11041 agttttccca ttggtccttc atggtaatcc aaggtgggag aaataaaagt taaaaagtga
    11101 ttaactaatt tagagtttac atagccaaat atttcttcaa agtcagttat atcctttgag
    11161 aacagaacag atcctttagt ttcaaaaatt gcctgattct gcaatctaaa agatacttgt
    11221 ccagatgaaa gtgtcgacca agtaattccc tttttaaaat agtaattaag attttgtggt
    11281 ctagatttta acttcccatt ttcattaaca taatttttta tttcatctcc atcattctcc
    11341 cagttaacca cgtattcatt gtttccatac cattttctaa aacgaccacc tttagttgta
    11401 ggaaaccatt tttttctaga ctttttagcg tcctctaatg ttttagcatc aaactttatt
    11461 ttgttaaatt ctacttcaaa ccaataccgc aaaaatttat tgttatttcc ggttgccaac
    11521 ccttgttttg catctgcaac atcgttagtt aattttcctt gagcaaaatc agtgattacg
    11581 ttatcgcttg cccagtaagc aatcggcatt cctgggattt ttttgaagtt gtcttgatta
    11641 gtactataaa aataatcgca ttcaccatgc tgatgaattg cttctaacgt tttaattttt
    11701 tgaacttcca ttccaccttt aaaatctgat aattttatat agcttccatc ttgttcattt
    11761 gaatttttta tcgtaaacgt acaaattgga acggttgctt cttcaaaagc tgaatactcc
    11821 atttgaatca aactagaaat gtttctttca ttaattaaaa atgagcgtaa cttttcatag
    11881 ctagaaatga acatccaaac aaatggcgtc ataaaggaag catatccatt atcaatcagc
    11941 atttggctat tcaatttaat aaacacagaa aatagatctt ttttgacatc agaatagtta
    12001 tcatttacat atttttttaa tttcttatcc attttattaa gataaggcgg attagtgact
    12061 accgctacat atttatcaac tagcatttca gccacgtcaa gaatgtgcaa gtatttttct
    12121 tgtatttcct tcatcccata aagaatatcc atattcgtaa attcgttgaa ttttgatata
    12181 tactctctta cttttcctaa gtattcttta gttattgcct gattattttt atcgactaac
    12241 ttaatgattg atccaagttc tttcccattt tcaaattgat caaaaattaa ctctttgtat
    12301 tcattccagc gttcactata cttatcattg ttcaattcac taatacgctc ttgatactcg
    12361 tctgtcactt taggagaatc aataaactct cttaaattcg gcgatagact ttttttcaaa
    12421 aaaagacgat cgtattgacg ccctttcatc ataattgcaa aataagacaa ttgatacgct
    12481 cttttatcga tttcaagacc atataaattg tattcaacga ttgcacgtgc agcatcacgc
    12541 tcagaatatc ctaacgtaag ataaatttcc atcaatacat cgaaggcgta aactaaaata
    12601 tgcccagacc ccattgcgtt atcgataatt tttagttctt ctggttttaa attacttaca
    12661 gatgttgctt gttcaaaagc atatttatct ggaattaagt atttcaactt atttcttaat
    12721 tcactattgg gataatgatc taaccaatac tttcctaaac tattgtcaac catataacga
    12781 acgacccaat ctgtggtaaa tagttgagtt gctgccggaa tatcttgttt tttgacaatc
    12841 cctttataaa tattcaccac ttgattatgt ggctcactat tatagtactg atacaaccac
    12901 ccaataatct ctacctgtcc accagcttga acatagaaat catcctctgg aatacttaaa
    12961 agatcagcga ttacaccatc ttttttaata taagaagctg taaacaaaag ttctgtataa
    13021 tcttctactt cttcaaatag ctcaggtaag taatcattca attgattaca gatttttata
    13081 aaaatcaatt gatacagctt gtccattgct tctgctgaat tatctgtttt ccaagttctg
    13141 acttgttgct gttcattact agataattct tcaaagatat cggcatcata aatatctgta
    13201 ataatatctg gctcaatttt cccacttatt tcactagata atacacgata cgtgtcaggt
    13261 aaataatcat ttacctccat aaaccgaatg gcaatcaatc ggttaaacca cgtataagca
    13321 acctcttcga ttaactcttg aaaagcttct gaataagaag tgacagaagc acgttgtttt
    13381 agttctgcaa tcaatttgtt tctttgcgct atttcttttc cttgaattct tattcctgat
    13441 ttttcgattt cttgtaaatt gctatcaatt ttcttcacat cttcaatttt tgattcatca
    13501 attcctaaac gttttgcttt agtcattact tcatcaatta attttctacg ggcattgacc
    13561 gcgaaattct ttatcgcttt cttatccatt tttctttccc ttctatatgt aacctttgaa
    13621 aagacaggca cctttttatg cctgtctttt cactagaatt gaatcgtgat ttctgtattt
    13681 tcttgaagtt gttccttcag tttttgatta atttcagctg ttaactcatc aatatctgct
    13741 gctgacttaa tagttttaac atgaacgtta agtgttctca ttggtactgt tttctgttga
    13801 atagtaggtt ttactatcgc tatttcttca gcttttgctg cagagtctat ttcatctttt
    13861 tcagtttcac gaatcaagtt tgctgcttca atttgtttac gtgcttgttc ttcttgacgt
    13921 ctgcggtttt cttcattatc gaaacgttga ttaaagatca aatactgttc tgctacgttt
    13981 aatgataaga aaacaagatc atctatagta ttcgcttgtt caatttttgc tttcagttct
    14041 tgaaattctt ggtcaactat tggtacaaat tgatccttaa aatcttttcc ttctaataat
    14101 ttaaatgttg cttgtttttc ttccgttaat ttatttaata tttcctgact ttccaactct
    14161 aacagttgat tatattgttc aataaattct tgaatcaact catttagttt catcatatct
    14221 gaataaggat cagccttttt caaaatggtt ttcatctcat gaatcaattg atcaatttct
    14281 tggctagaag tttgacgccg acttttttcg attaatctaa ttttatcttg tgcattctcc
    14341 cagatttttt tctgagtact attttgcttg tggaaatttt taattttatc atatgtttca
    14401 gcccagtcga ataattcatc gcgatatttt ttgattgtat aaaaaaagtc ttcaacatct
    14461 gacgtttgaa caattacttc aagatatttt tttatttgct catattcata agcactaggg
    14521 taggtataag tgttttgtaa atcaaaataa gagcgtaaat tgtctaattg accttttaaa
    14581 tatttcttga aattttccat tgtatgttca ttgttatttt catcaacaat attacgactt
    14641 tgaaacagtt ctctaccgat ttcgtcaacc aatttttttg cttttggcga aatcaccgcg
    14701 cttggttcaa tttttatctt ttcactttct cgtcgttttt gaataatcat tacaatacta
    14761 tttggatcat ccatccgtga aatttgtaca ttgttataaa gtaaatcaat tttaccagac
    14821 atgaaaagtt tagcgatgca ccattccaca tcaagttctg tgaatccata cggttgtttt
    14881 gtttcaaaac gatcacgaat tgttttcatc gtaatcgtta ggtgatttct cttttgatca
    14941 ttgataaaat tgaaaatttc ctgaactgct tgctcatttg atggatcttc aataatttca
    15001 tttttgcttt ctttcactaa atataagata tcttcttttt ctttaggcgc atcaatttgg
    15061 tttaatttat ggtatacttc ttcagccatt ttctgaagtg ctgattggaa ttttgcttta
    15121 aagtcggagc ctgtcagttg aatttctgca ccgttataga aaaattttgc agatttcaac
    15181 agttcattta attgatcgtt gatacttgca cttagttcct gtaattcacc agatttttcc
    15241 gaacgaatcg cgtcaaagtt ctttactaca ctcgaataag tcgttgttaa ataaagattc
    15301 attctagcat acattgctat atcatcttta aattctttat ctgatggcaa ttctacgaat
    15361 aataagttac gggaactctt aagcgcaagt tctgcctctt gacctgctaa atcagagtat
    15421 ggtgaaataa aattaactgt catttcagca tttccagctt gcttgtatgg acgttgatct
    15481 aacacttgtg cataagtaaa cgtataccgg tttttaaatt tatttccacc tgacatgcct
    15541 aatttttcat aggcaaaact atttcggtct agaacgccgt caaaaattcg tttagatagt
    15601 tctacgatca aatcactatc tgtaatattt tgatttttaa tctcacgagt aatttcctgt
    15661 tcttcatcag tttggaaaat ataactatct aagttcttcc gcacaaagtt ttccttaatt
    15721 aaaaggtcca atgcttcttc tactcgtttt ttagtttcca ggcggtcttc gttgatattt
    15781 cttatcatca agctcgtaat attttcaaca tttggtttga tggcttgatc aacatattta
    15841 atcatgaata aaattcgtaa gacatttacg ttaaagttat ctatatcagt tggtctttga
    15901 atatgagaat tatttgtagc ttgtgtaatc actcggctaa ttgctgtatc aatccaacgt
    15961 tctaacgagt aataaaactg atctaaagaa accaaactgg ttgtatcatt ttccttattt
    16021 cgttcagcgg cttctttaaa cattgctagt aatgaacgtt caccagacga taagtttgat
    16081 ccttgatagc tgtgattacg aatctcggtt agaatttgcc ccaataaatt gaattgatag
    16141 ggaataaatg gataaacttc tgcgtaattt tcagcatctt ggaagagctt taattcagga
    16201 ctatcaacaa agagtaattt atttttcaat tctgtttgaa cagtttcaaa ttgtaaggct
    16261 aaagaatcat tggcaaactc ttttttcttc aaaatacgtt ctttaattac ttcatcggca
    16321 tttgctgaag ataaactcac agttgtggca aaacgatcct tgatcttaga aaaatcatta
    16381 tcagaattgt tatcaaagtt tgttacgtga tcaatcgctt cctgacttgt cactaatacc
    16441 caagccttac cttcacaaat tcgacccaaa tcttccacaa cggtttgtaa ctgcaaagtc
    16501 aactcacgat taccgccgat atattgtccg acctcatcga ctagaaaggc aatatgatgg
    16561 tctgcttctt tggaatcaag atagctttta actagatttg caaagtcatc aatgcttatt
    16621 aaataatttc ctgatgcttt ttctaaaaaa ttatcaatag cggcttcact cataaaatcg
    16681 attttaagtg ccgcttcttt gatatcatct tggatccaat caaactcatg gcgctcttct
    16741 tgccattttg ccccattaat ctcttcaaaa gcagctttga atgcatcaaa cttttcttct
    16801 ttaattaacc gacgttcaaa atctgctaga tgtggaatcg tgcttaatcc ttgcatttcg
    16861 ttaaatacct ttaaaaaaac attcacgatt ccgtttttgt cttttttacc acctgcatca
    16921 ctttttgaat caatattaaa cagaataaca tctgtattta tttgtgagca ccgttccatc
    16981 gttgccaaag tcagtggatc tttgattttc ccatcttctt tgaagtaatc aatggctttc
    17041 ttgtcattta tttctcgatt tgcgagcaag taagaaataa tttttaagaa atgagattta
    17101 cctgaaccaa agaacccctg aatccaaaca cccattttat ctgttggtgt attgatactc
    17161 cgttggtagg catcaaaaat tgttttaaag tgtttttgta gttctttcgt aaccacgtat
    17221 tcttctaatt cttgttgaac attttcatcc tttgcttgac cgaccttaat aactccttga
    17281 atatttcggt caattggttt tgcaaaaata tctttaatta gcatttaatc tcctaccttc
    17341 catcccaact aaattctaaa tgctcgataa taattatcat ctttattggt attaagaatt
    17401 gtcaaactta attcatcata ttcccctgga tacatcatca caactggtac gcgatcaaat
    17461 acctgatgca ttttatttaa aatagagtgc gctctaatta atggatataa ttcgccaatt
    17521 cctgtcagga acacaactga tttttctggt gtatgggcat ccacatattc aaaaaataca
    17581 ttttcttcct caggtaaatc cattttcaat aaacgaccaa tgctttccac taatttttgt
    17641 aaaccacgtc gttcttccat cttaatcgct ttttcagtga aaccgcgttt ttctagatac
    17701 tctaacataa tcacatagag attataaact ttcacttcat aattatcacc aacagatgtt
    17761 ccagatatat cattaatata atctcttaca attaactcgt ctttcggatc gtagtcaaag
    17821 atataatagc ctacttcatt acctaaacca cgattcaata aaaaatcttc tttttgaatt
    17881 acttcattca acttttccaa atgctcttga atcgataatg acattataaa attcctccca
    17941 gtgctagtac aatatcttta cgattcttgt caactaacca ttgaatcaat cgtttatcta
    18001 ataatatcgg agtcattcgt ccatattctt tttcccctct gactaatcct gtttcaatta
    18061 gattcgtttg ataagcggca ataagccgtt taatcgtata atctttccat tttgaaatat
    18121 cttctgaact tctccgcttc tctgtaaaaa aagaacggta atcaactggt gttaattgca
    18181 aatttccttt acgtagattt tcagcatata cctcagtcat aaattcatta aaaagatcat
    18241 caatcataag gatggaaatc aaattcacta acttttgatt atctatattt aactgattga
    18301 aaagttgcaa tccagcttca tccagttgac taactctcct tttcacgttc tgatagattt
    18361 ctttttgacg gttactgctc actgcaaaaa aaatattttc atgttcattt ttctctttaa
    18421 tttctatatc tgtatatcct tctagtcgta attgaatata taacttgaac tctgaaaacc
    18481 aaaagcgttt agaaactaga ccagcagaat attttttatt caatagattg cctcctaatc
    18541 atctataatt caataagtaa acaagtgttt tttgctcatc gttagcattc attttttagt
    18601 atctactata caatacatat ttattata
//
LOCUS       DAZKOM010000051        18567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000051 DAZKOM010000000
VERSION     DAZKOM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.051"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8NF_001690"
     CDS             6..305
                     /locus_tag="ACG8NF_001690"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0472173.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8NF_001691"
     CDS             378..1739
                     /locus_tag="ACG8NF_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0472174.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8NF_001692"
     CDS             1761..2021
                     /locus_tag="ACG8NF_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472175.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8NF_001693"
     CDS             2035..2787
                     /locus_tag="ACG8NF_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0472176.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8NF_001694"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8NF_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0472177.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8NF_001695"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8NF_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0472178.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8NF_001696"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8NF_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0472179.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8NF_001697"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8NF_001697"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0472180.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8NF_001698"
     CDS             7161..8558
                     /locus_tag="ACG8NF_001698"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0472181.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8NF_001699"
     CDS             8639..9079
                     /locus_tag="ACG8NF_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0472182.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8NF_001700"
     CDS             9227..9553
                     /locus_tag="ACG8NF_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0472183.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8NF_001701"
     CDS             9554..10303
                     /locus_tag="ACG8NF_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0472184.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8NF_001702"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8NF_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0472185.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8NF_001703"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8NF_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472186.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8NF_001704"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8NF_001704"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0472187.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8NF_001705"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8NF_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0472188.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8NF_001706"
     CDS             14770..17436
                     /locus_tag="ACG8NF_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0472189.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8NF_001707"
     CDS             17800..18552
                     /locus_tag="ACG8NF_001707"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0472190.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5965 a         3212 c         3833 g         5557 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatg
//
LOCUS       DAZKOM010000052        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000052 DAZKOM010000000
VERSION     DAZKOM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.052"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8NF_001708"
     CDS             99..746
                     /locus_tag="ACG8NF_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0472191.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8NF_001709"
     CDS             1139..3784
                     /locus_tag="ACG8NF_001709"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0472192.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8NF_001710"
     CDS             4094..5407
                     /locus_tag="ACG8NF_001710"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0472193.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8NF_001711"
     CDS             5397..6050
                     /locus_tag="ACG8NF_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0472194.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8NF_001712"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8NF_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0472195.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8NF_001713"
     CDS             6947..7147
                     /locus_tag="ACG8NF_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0472196.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8NF_001714"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8NF_001714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0472197.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8NF_001715"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8NF_001715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0472198.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8NF_001716"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8NF_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0472199.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8NF_001717"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8NF_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0472200.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8NF_001718"
     CDS             13269..13466
                     /locus_tag="ACG8NF_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472201.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8NF_001719"
     CDS             13619..14104
                     /locus_tag="ACG8NF_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0472202.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8NF_001720"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8NF_001720"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0472203.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG8NF_001721"
     CDS             14981..15508
                     /locus_tag="ACG8NF_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472204.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG8NF_001722"
     CDS             15599..16336
                     /locus_tag="ACG8NF_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0472205.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8NF_001723"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8NF_001723"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0472206.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8NF_001724"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8NF_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0472207.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKOM010000053        18360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000053 DAZKOM010000000
VERSION     DAZKOM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.053"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      95..261
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            409..966
                     /locus_tag="ACG8NF_001725"
                     /pseudo
     CDS             409..966
                     /locus_tag="ACG8NF_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1024..3066
                     /locus_tag="ACG8NF_001726"
     CDS             1024..3066
                     /locus_tag="ACG8NF_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0472208.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADDKETKDKKNVISETAVQTIVSDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3175..4326)
                     /locus_tag="ACG8NF_001727"
     CDS             complement(3175..4326)
                     /locus_tag="ACG8NF_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0472209.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4480..5010)
                     /locus_tag="ACG8NF_001728"
     CDS             complement(4480..5010)
                     /locus_tag="ACG8NF_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0472210.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5109..5459)
                     /locus_tag="ACG8NF_001729"
     CDS             complement(5109..5459)
                     /locus_tag="ACG8NF_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0472211.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5616..7145)
                     /gene="ahpF"
                     /locus_tag="ACG8NF_001730"
     CDS             complement(5616..7145)
                     /gene="ahpF"
                     /locus_tag="ACG8NF_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0472212.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7155..7718)
                     /gene="ahpC"
                     /locus_tag="ACG8NF_001731"
     CDS             complement(7155..7718)
                     /gene="ahpC"
                     /locus_tag="ACG8NF_001731"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0472213.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7862..8524)
                     /locus_tag="ACG8NF_001732"
     CDS             complement(7862..8524)
                     /locus_tag="ACG8NF_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0472214.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8708..10354
                     /locus_tag="ACG8NF_001733"
     CDS             8708..10354
                     /locus_tag="ACG8NF_001733"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0472215.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10345..11427
                     /gene="uxuA"
                     /locus_tag="ACG8NF_001734"
     CDS             10345..11427
                     /gene="uxuA"
                     /locus_tag="ACG8NF_001734"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0472216.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11535..13088)
                     /locus_tag="ACG8NF_001735"
                     /pseudo
     CDS             complement(11535..13088)
                     /locus_tag="ACG8NF_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13236..14377
                     /locus_tag="ACG8NF_001736"
                     /pseudo
     CDS             13236..14377
                     /locus_tag="ACG8NF_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14503..15582)
                     /locus_tag="ACG8NF_001737"
     CDS             complement(14503..15582)
                     /locus_tag="ACG8NF_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0472217.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15691..16131)
                     /locus_tag="ACG8NF_001738"
     CDS             complement(15691..16131)
                     /locus_tag="ACG8NF_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0472218.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16188..16598)
                     /locus_tag="ACG8NF_001739"
     CDS             complement(16188..16598)
                     /locus_tag="ACG8NF_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0472219.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16779..17213)
                     /locus_tag="ACG8NF_001740"
     CDS             complement(16779..17213)
                     /locus_tag="ACG8NF_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0472220.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17313..18161)
                     /locus_tag="ACG8NF_001741"
     CDS             complement(17313..18161)
                     /locus_tag="ACG8NF_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0472221.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6308 a         3390 c         3300 g         5362 t
ORIGIN      
        1 aatattaaag attgttaaat ttcactattt catttcaaat tccattgcct aatcattagg
       61 aaaagtctat catgaactcg taaatagaat atagtaataa ccttgttagg tgaggctcct
      121 atacaaacat aggctgttgc ccagaaacgt cgaaagtcgc caatgggtaa aacagaaact
      181 gttggattaa ggctttttcc aatagctaag aatgttttct ttacgttgta tagtgctaaa
      241 actcggacga tgagatgatg ctaggtcaga attcttgttc gaaaaggttt ctgacctttt
      301 ctttatattt ttgtgactga cttatttgat gagacaaact cgctcctcac ctgacaagtg
      361 gattgctgtg tgaggaggag atgcggtgaa aaaagatcga ttggtggagg tcgctcggat
      421 tcacttctgg ttgcacaata caaccagaaa acacaacaag caaaactgat ctctatcatg
      481 agagattcat atgtcgatat tccaggttac ggaatgaata agataaatgc agcttactct
      541 tatgggggag ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt tgaagctccc
      601 tattatgcaa gtatcacatt tcaagatttt atcgattgcg ttaatgaact gtttcctggt
      661 ggagtcaaga tcgatgcaga aaaatctcta gatttagatg gcgtatatat aaacaaagga
      721 gagcaagtga tggatggtaa tactttactc cagtatgctc gatttcggga agacaaagaa
      781 ggagacttcg ggaggatcag aaggcagcag caagtgatca aagctatctc tcagcagctg
      841 aaagacgtaa cttcaatatt caagctaccc aaagcggttg ggaaattact tggtagtatc
      901 caaacgaacc ttccagaaag tgtgcttctg gattgtggta tggatttcct caaagacata
      961 tgttagaata aacaggtata aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa
     1021 aaaatgaaaa gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat tgccggcgca
     1081 gtgatcttaa tagcagctgc gggtggcggt tatttttact accagcacta tcaagaaacc
     1141 caagcagtag aagctggaga aaagacagtt gagcaatttg tccaagcttt gaacaaggga
     1201 gattataaca aagctgcaga aatgacatca aaaaaggcgg caaataaaag tgcattatct
     1261 gaaaaagaga tcttagataa ataccagaat atatacggtg ctgctgatgt caaaggactt
     1321 cagatatcga atctaaaagt agataaaaaa gacgattcta cttacagttt ttcatataaa
     1381 gcaaagatga atacctcatt aggtgaattg aaagatcttt cttataaagg aacattagac
     1441 agaaatgatg gtcaaaccac gatcaactgg caacccaact tggtttttcc agaaatggaa
     1501 ggaaatgaca aagtaagcct gaccacacaa gaagcagcaa gagggaatat tatagatcga
     1561 aatggtgaac cattagcaac aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa
     1621 cttggagatg gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc cttcgactta
     1681 acagaagatg ctatcaatca ggcgatttca caaagctggg tacaacccga ttactttgtc
     1741 ccattgaaaa tcattgatgg agcaacgcca gaacttccag ctggagctac cattcaagaa
     1801 gtagacggca gatattatcc tttgggtgaa gcagctgctc agctgattgg ttacgtggga
     1861 gatatcacag cagaagatat tgataagaat ccagaattaa gcagtaatgg aaaaatcggt
     1921 cgctcaggtt tggagatggc ttttgataag gatcttcgtg ggacaacagg tgggaaatta
     1981 agcattacag atgcagacgg tgtagagaaa aaagttctga tcgaacatga agttcaaaat
     2041 ggcaaagata tcaaattgac aatcgatgcg aaggcacaaa aaatagcttt tgacagtcta
     2101 ggaggaaaag ctgcatcaac tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt
     2161 gctagctctc caagctatga tccaaacaaa atgacaaacg ggatctcaca agaagattac
     2221 aaagcttatg aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac aggttatgct
     2281 cctggatcta cgtttaaaat gattacagca gcaatcggtc tcgacaacgg cactatcgat
     2341 ccaaatgaag tgttgacgat caacgggctt aaatggcaaa aagacagttc ttggggatct
     2401 tttaaaacaa cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac tgctttgatc
     2461 tattccgata atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt
     2521 acaggcttag ataaattcat ttttggtgaa gaccttgatt tgccaattag tatgaatcca
     2581 gcacaaattt ctaatgaaga tagctttaac tcagatatct tgctagctga tactggatat
     2641 ggacagggcg aacttctaat tactcctatc cagcaagcag caatgtattc tgtttttacc
     2701 aacaatggca cacttgtcta tcctaaattg attgcagatg ataaagagac aaaagataag
     2761 aagaatgtca tcagcgaaac agcagtacaa acgatcgtgt cagatctgag agaagttgtg
     2821 caagatgtaa atggtacagc acattctctt tctgctttag ggattccgtt ggcagcgaaa
     2881 actggtacag cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg
     2941 tttgctttca atcttgataa ccaaggttat atgatggtca gcatgttgga aaataaagaa
     3001 gatgatgatt cagcaaccaa acgagcatcg gaactattgc aatacctcaa ccaaaattat
     3061 caataaaaaa ttgcttttta agtacagtaa aaggactgtg gcgttatcgt cacagtcctt
     3121 ttttcaaata aacggttttc acgatgtctc tttcacaact tctttttgat ttactcacac
     3181 ttcataaact ttttttgtaa aatatttgag aacaagtgga gaaataagtg tacttaataa
     3241 aatcacgata actaaaggtg aatagtactg tggttcgatc aattgacttt gctgtccaat
     3301 ttgtaaaatg atcaaagcca tttctccacg cgaaatcata ccagcaccga ccatcaatga
     3361 actatgttga gaaaaaccgg caatctgagc accaaaataa cctccaagca atttggtcaa
     3421 aatcgctaca agcgtcaata tagaaataaa gagtagttgt tcactaaagt cagaaaaatc
     3481 aacttctaat cctacactga caaaaaatac tggtataaat actgcatatc ctaaagcctc
     3541 tacattgtgg tatacttctt ctttaacctt tgtctgactg acagctatcc cagcaaaaaa
     3601 agcaccaatg acagagctaa gcccaacaag atccgctacg taagacattc ctaaacaaat
     3661 aactagcgat acgatgataa tagaagaatt tgcgtacact ttttctgcaa atgaaagtag
     3721 gaaaggcgca atccatttga ctaataagaa aataaaaaca aaataaatta gctgttcaat
     3781 caaaacaagc ggtaaagaag cattgctaga agcttccccc gttagaaaag ataggctgaa
     3841 acttagaact aaaacgacta aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc
     3901 ttcttttgta ttcactacgt ttagttcttt taatacttct actgaaatac tgacagaagt
     3961 tgccgcaaga ataatcccaa agaaaatcgc ttcatttccc gaaacttgga atgctttgcc
     4021 tgtcaaccag ccaaaaacaa ccgggaagat gatcccaagc aaagcaacga acataccagg
     4081 tcggaaatat tttttcaaaa gagataaatc actttccaga ccagctaaaa acattaataa
     4141 gatcacacca atctccgaaa agtcatggac tagaatatca ggtttgaccc aattcaatcc
     4201 agcttggcct agcaaaacac caacaagtaa ttgtccaatc actgctggga tacctaatct
     4261 ccgcgagata tgtgagccaa tcgttgttga aatcaatatc aggcataaaa taccaataaa
     4321 ttccattctc atcattcctt tgtataaaat atagcccgaa cttagcataa catacattat
     4381 tgaaaaaata ggaaaatata ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt
     4441 gttggttttt atacggtcta ttttaataac taaaaatgat taacgtatag tcatttttct
     4501 tattctgatg aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat
     4561 ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa
     4621 caacagaata ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa
     4681 ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat
     4741 tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag
     4801 accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa
     4861 cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg
     4921 gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt
     4981 ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac
     5041 gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc
     5101 tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc
     5161 attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca
     5221 ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa
     5281 aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc
     5341 gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt
     5401 gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata
     5461 atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa
     5521 gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt
     5581 ttttccgctc tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat
     5641 gctcctaaag ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta
     5701 tctgtacaat caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc
     5761 actatttcat ttcttggatt catttcgatc gttctatcta gccaatccgt attagggagt
     5821 aagccgacta gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga
     5881 tcgatatagg agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt
     5941 ttgataacag tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt
     6001 aattctggta agaattctag aacatataca tgttttgcta acccagccaa atcgattgct
     6061 gcttcaacac cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg
     6121 ccgtcacagt gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca
     6181 ttgatagaac gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata
     6241 ccgttagcta agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga
     6301 ttcatcataa tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt
     6361 ggaccttctg tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt
     6421 ccgccgaaag aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct
     6481 gcactactag ctgctggtcc accgccaatc actaatacat cataaggttc tttttcaaac
     6541 aactcttctt caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt
     6601 ccgctactaa attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca
     6661 tcgatttctt tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat
     6721 acagctaata tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca
     6781 taactgtcaa aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg
     6841 attttcggtg cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga
     6901 ccgaggggga taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa
     6961 ctaggtgtgc gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt
     7021 tccttcgtaa attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa
     7081 acaataggag attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct
     7141 aacatatcca ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca
     7201 ctttctttcc attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg
     7261 cgaatcttat caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata
     7321 gcttgaacga ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct
     7381 tcatccaaaa catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat
     7441 tcgattttac cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg
     7501 gtagagcaag aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag
     7561 tcttctaatt cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc
     7621 cagttcccca taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt
     7681 gcattaaatt cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt
     7741 atttatttat aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt
     7801 taataaagat tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat
     7861 gctactgctg gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca
     7921 tgtgtaagtg caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct
     7981 tgagtaaatc ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc
     8041 gtaaccagcg gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt
     8101 ttcttcctgc aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc
     8161 gttcgtcatt tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc
     8221 gtgtttgtgt gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc
     8281 ggacaaagcg agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt
     8341 gaggtatcgt atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag
     8401 tccggccgat agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta
     8461 agtcagcata gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt
     8521 tcatctttat tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa
     8581 acaatctggt atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca
     8641 tagcgatttt tattgcataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag
     8701 gggaaaaatg attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga
     8761 agtagaaata cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt
     8821 ctggatccat ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg
     8881 tttagcggat ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga
     8941 aataattgaa agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag
     9001 cagtggagag ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc
     9061 agattgttct gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt
     9121 agttactttt gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat
     9181 aggaccgatt gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat
     9241 tcttgctgtg ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc
     9301 tacagataat ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa
     9361 agaatggcaa aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga
     9421 aattgttgcg tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt
     9481 tagcaaaatg ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg
     9541 aaataatatt gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca
     9601 ttttcccaat ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac
     9661 ggtagataaa gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt
     9721 agctattatg ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga
     9781 tttatcgctg ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc
     9841 aggtatcatt gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc
     9901 aaatattcct aatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg
     9961 ttatggtgag acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt
    10021 attcatacca ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg
    10081 aaaaccattc atcccgagtc cagacccact attgacagac cttcagcata agctttccga
    10141 tattcaactg ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc
    10201 aaataaagaa atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga
    10261 aatgttcatc aagatggtag caggaccaaa tgccgtacga aaaacattac aggattactt
    10321 acaagaacat ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa
    10381 gaaaagatta ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca
    10441 ttgatggata taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa
    10501 gaaattgagt ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc
    10561 attaaaattg gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga
    10621 aacttggcag aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg
    10681 gtgaaatctg accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct
    10741 gatgatattc cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa
    10801 tttggattgc cgggatggga acctgagcgc ttagctccta tcaaatcctt gcttgaagcc
    10861 tatgcatcag tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc
    10921 atcccaacat gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac
    10981 tcgatttttg gattagctcg aatcgtaaag aatcgagaag atttagattg gttgtgtcgc
    11041 atagtagaca gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca
    11101 gaaaatgatg tatatgggat tttagcagaa ttttgccaaa gagatcgaat cccatttgca
    11161 catgttcgaa atattaaatt tttagctaat cgagatttct atgaggcacc acataaatcc
    11221 gaatacggtt cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac
    11281 ggatacatcc gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac
    11341 ggcttatatg atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa
    11401 aaaggaaaca aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag
    11461 aaggatgttc aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat
    11521 gtatcttatt tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct
    11581 tcttctttta attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct
    11641 ggtgtgatga ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa
    11701 gggcagtcgc tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat
    11761 tcatttgaaa aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc
    11821 gtatagttca caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact
    11881 aggtcttcaa ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga
    11941 ttttgttctc tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaatacg
    12001 ctatgttctt gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga
    12061 aatccattat aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca
    12121 ggtataaaca aatcaaatac agtacaaagg gactccacca tttctcttga ataaagaaca
    12181 tgaggaacgt ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg
    12241 attttcgtga tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt
    12301 ggcagattag gtcgcaaaat atctacagag agatcaataa aaaagtcaat atattcatac
    12361 tctgtgaatt ctttttgtac gtctttcaca gaagttgcag gaaaattctc tttacatatt
    12421 tttaatagaa aatataattt atctatcata ccgctattta gtcggtcaag tacttctttt
    12481 ttaaaaaaag tattcatcat aaaagccact acttgcggat cttcatcata aaattctgta
    12541 gcagtacttg aaaagatgtt ccaataaata tctaatgctg catattgaat ttgtacctct
    12601 tttccagaaa aagactttct gccattatta gaaaaggaga tagacaaacc gtactatgct
    12661 aaaaatgcat taatcttttt cattttcgta tacaaataag aggcactata gtttgtttgc
    12721 tgactgaaac tgataatcga atttgtctga cagctgaaca aagaagtcaa gatctgataa
    12781 ctgactgatt cgttgcagta tcttccaaat agacgataat agcagtaagc cggatcaatt
    12841 ccttcaaaat tcaactttaa aaaaggtttt tgcttatcaa tatgcagttc tgttagattg
    12901 agacgctcaa agtcttgttc taattctttg atgtatagta aaaaatttga ttcagacata
    12961 tggaaatgat ctgctaaata ttgtgtagta ataaagaaac gatgttcaga taggaaaaga
    13021 aaaatatcta attttgtatt gctttttttc ttcaagataa acgttttaat cgcttcttga
    13081 gtttgcatga aagaggctcc tttttactta aattgttaaa acttgaaaat attactgttt
    13141 ttttcataaa tttagacaaa aaaacagtaa acatataggt agaatctcta ctatcttatt
    13201 tcttgcatac aaaaaaatca aggagtgaga gagttatgag atattcaacc atcttaatat
    13261 caacatttgc agccttaagc ttatcgctta ctataaatca gacagtccac acagaggaga
    13321 cagaaacagc caaatggaca gaaaccaagt gggccaacgt caatcgaatt acctttcagg
    13381 atgaattttc atccttcaat tcaggagtcg caaaattaag aaaaggacaa acagcgacca
    13441 tcaaagcaaa aattgactat tcgggagact tagcacctat actacaatca gctgttacat
    13501 ttgagaatgt tgatcccact ttgtcgattg gctatgacga gaacactttg actatccaac
    13561 acaagcaagc gatatttacc ttcaccgtta cattaaatca ggacttgtta aaaccagcgc
    13621 tttttactgt caaggtatca gatggactac cgaacgatca tcatttaacg ccgtatagtc
    13681 agcggcagac aattgagcaa gattcagcca tcgactcagg aggagatctt gttgaagaac
    13741 caacagataa accagaaaat gaaaataagc cagaagtacc gcctacagaa aatccagacg
    13801 gagaacagaa accggaaata gaacctggag aagaacccga tacagaaact caaccagagc
    13861 cagacaataa atcaaaacct gaaataaccc ctggagaaaa accagatgta actccagaac
    13921 cagatacgga ttctggaaac caaacagtac cggaaacaaa tccagacaca gataatgaaa
    13981 ctgaaaatcc agaaaaacca gaggtagatc cagaagaaaa accagatgtg actccagaac
    14041 cagatacgga cgcaagagac caaggaatac cagaaaaaat aaacaaaaaa acaattcaag
    14101 aggatggaaa aaaagagagt aaaaaatcaa atctagcaat tctaaaaata aatgaagagc
    14161 agctgaataa aaaatcacga atatttgata gcgcccaatc agctgaaacc ttaaaaagta
    14221 gcaaggatac aacctttgct gtcctgaaac gaaaaataaa cagctaccaa aatccggaga
    14281 gtcacaaaac aaagttatac tgtggagcgg aatcattcta ttaagtatag caacaatgct
    14341 ttctgcaaaa agatttaaac aaaacagatc tctctaacat ttgaataatg tctgtgaacg
    14401 catttttcaa ataaaaagaa atatccagaa aatgacccca aaataccaga ttgaaatctg
    14461 atatttgggg tcgttcatac tttatctttc ttcatattta ttttaaggga tatctgtttc
    14521 ttttggaaga tggttcggga tctttttcac tttcaataca tctaaaaaga aggtaaagat
    14581 cggaatgccg acaatcaagc cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac
    14641 aaatgtataa aaaatcggca gttctgtttt gctggacatc aacttcggat tcagcacata
    14701 agactcaatt agatggacaa tcaaaatcgt aatcaggata taaacaacat ctcggatgcc
    14761 tccttgggaa taagctatga aagatagagg aatacaagaa atgataacac ctgctactgg
    14821 gatcagactt aaaatgaaaa tcatgatcgc taaactcagc agctgatgaa agccaaatac
    14881 agccaaggcg attgtagtga gcaacgtatt taaaagagcg acaacaaatt gagcttctaa
    14941 taccaaacca aatgtattga caaacttatc ggcaaaatag taaatatctt gaaagaacca
    15001 agaaaattca ctttttaaaa acaatcttga gaatacaatt gtttttttct tttcaatcat
    15061 aaaaaagaaa cttaagatga atgacatagc aaacgccact gccatttttc ctatatcctg
    15121 taaatagcca agcgcaatcg aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc
    15181 aatataagta tggatatatt gtaaaacagg atctgcatct tttggctgat gctcgtaaaa
    15241 atcaataacg gactggatca tatcaaatgt ttgatgaata agcatcggga tatactttgt
    15301 gaatgccaga tagataaggt aaacgatcag tgtataaatc atgatagcta aaacacctgt
    15361 agggattcgg acaaatcgct gaatgatttg aacaagatga atcatcaaat aagtaaagat
    15421 aaatgtgagc aaaatggttg taatcatact tctaactaag aaaagaacaa aaacgacaaa
    15481 taataaaaca gtgagacggc gtattttttc attcgccaaa aaacgctgat aaacggtcaa
    15541 atcgacaacc tccaaataac aaaaaataaa cacgttattc attatatgat ggaatatgaa
    15601 aataagaaag agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt
    15661 tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt
    15721 atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag
    15781 ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagg
    15841 agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt
    15901 tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt
    15961 tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg
    16021 ggtataggta agattcagcg gcttgaggag ggaagtgtag tgtctaatga tttccttaga
    16081 ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc
    16141 tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact
    16201 gctttcagag taacttctat atttccagct gtagcttttg aatacggaca cactttatgc
    16261 gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc
    16321 tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca
    16381 gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat
    16441 gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca
    16501 ggagctgcaa tattcaattc gaatgtacga tcgggattat gaacttctcc agagcgtccg
    16561 cctgtgttga tcattttcgt agaatacatt tttttcataa aattaccacc tttaattata
    16621 ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata
    16681 agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa
    16741 aaaaatatca gagacaaaaa ctaatctcat cataaacatt acatatattt ctcaaaaagt
    16801 tgttttcttt cttcaaaact tacttcacga tggacctgat gaagcatcca taaataacca
    16861 aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt catttcttga
    16921 atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc agagacgatg
    16981 atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc aagagacgtg
    17041 ttttcatcca gcatatggaa tctcgtgcca tacaaggtaa aaatgacttc attattgcct
    17101 ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg atacagttct
    17161 agtgctttca aactatcatt taatacaaaa tctatttccg ttcctttatg cataaagatc
    17221 tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagtt aaagaaaacg
    17281 cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata agtaaatccg
    17341 cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc gatttcttct
    17401 atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa aacctctttt
    17461 gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc aatctcagca
    17521 cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac agatttcttt
    17581 cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat agataaaata
    17641 gcataaagaa actcttctat tccaaaaaca aacagattaa gagaaacaac gaatgcgtct
    17701 gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat gaggggaggg
    17761 atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc taaagcaaaa
    17821 atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt ttctggaact
    17881 attgcaatga ttaaaggaaa aaacaagctt ccgaaaagta ctttaaaaaa gggtttttta
    17941 cctaaaaata gaagagctag taccaacata acgatattca acactaaaat aactatagct
    18001 cggtcaaatc ctaaaacagc ttccagtaat attcctagac cgctgacacc tccagcagca
    18061 atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag ggcaaaaata
    18121 atcttaatgt atgtccatat aaattgtctc gtttctttca tttatccact tccttaattc
    18181 attaataata ctcttatctt attataactt acttttcacc ttaagttaac tttaattaat
    18241 aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaatata cttctacgtt
    18301 aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct taagataaat
//
LOCUS       DAZKOM010000054        17989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000054 DAZKOM010000000
VERSION     DAZKOM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.054"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1785
                     /locus_tag="ACG8NF_001742"
     CDS             85..1785
                     /locus_tag="ACG8NF_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0472222.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1755..1973
                     /locus_tag="ACG8NF_001743"
     CDS             1755..1973
                     /locus_tag="ACG8NF_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0472223.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1993..2883
                     /locus_tag="ACG8NF_001744"
     CDS             1993..2883
                     /locus_tag="ACG8NF_001744"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0472224.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2901..3185
                     /locus_tag="ACG8NF_001745"
     CDS             2901..3185
                     /locus_tag="ACG8NF_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472225.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3672..4286
                     /gene="gmk"
                     /locus_tag="ACG8NF_001746"
     CDS             3672..4286
                     /gene="gmk"
                     /locus_tag="ACG8NF_001746"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0472226.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4291..4596
                     /gene="rpoZ"
                     /locus_tag="ACG8NF_001747"
     CDS             4291..4596
                     /gene="rpoZ"
                     /locus_tag="ACG8NF_001747"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0472227.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4849..7257
                     /gene="priA"
                     /locus_tag="ACG8NF_001748"
     CDS             4849..7257
                     /gene="priA"
                     /locus_tag="ACG8NF_001748"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0472228.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7272..7763
                     /gene="def"
                     /locus_tag="ACG8NF_001749"
     CDS             7272..7763
                     /gene="def"
                     /locus_tag="ACG8NF_001749"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0472229.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7756..8694
                     /gene="fmt"
                     /locus_tag="ACG8NF_001750"
     CDS             7756..8694
                     /gene="fmt"
                     /locus_tag="ACG8NF_001750"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0472230.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8694..10052
                     /gene="rsmB"
                     /locus_tag="ACG8NF_001751"
     CDS             8694..10052
                     /gene="rsmB"
                     /locus_tag="ACG8NF_001751"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0472231.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10065..10805
                     /locus_tag="ACG8NF_001752"
     CDS             10065..10805
                     /locus_tag="ACG8NF_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0472232.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10802..12871
                     /gene="pknB"
                     /locus_tag="ACG8NF_001753"
     CDS             10802..12871
                     /gene="pknB"
                     /locus_tag="ACG8NF_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0472233.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13036..13935
                     /gene="rsgA"
                     /locus_tag="ACG8NF_001754"
     CDS             13036..13935
                     /gene="rsgA"
                     /locus_tag="ACG8NF_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0472234.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13948..14598
                     /gene="rpe"
                     /locus_tag="ACG8NF_001755"
     CDS             13948..14598
                     /gene="rpe"
                     /locus_tag="ACG8NF_001755"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0472235.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14599..15237
                     /locus_tag="ACG8NF_001756"
     CDS             14599..15237
                     /locus_tag="ACG8NF_001756"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0472236.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15392..16246
                     /gene="mreC"
                     /locus_tag="ACG8NF_001757"
     CDS             15392..16246
                     /gene="mreC"
                     /locus_tag="ACG8NF_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0472237.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16250..16759
                     /gene="mreD"
                     /locus_tag="ACG8NF_001758"
     CDS             16250..16759
                     /gene="mreD"
                     /locus_tag="ACG8NF_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0472238.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17014..>17989
                     /locus_tag="ACG8NF_001759"
     CDS             17014..>17989
                     /locus_tag="ACG8NF_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0472239.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         6110 a         3267 c         3896 g         4716 t
ORIGIN      
        1 ttcttatgga atctacaaag tcgaatacga gactcatgac ctttttatga cgtactggta
       61 tacttgaaga aaaaggagag cgcgatggag ataaaaaaag tttacttttc acagaaaatg
      121 tcgctgtatt cgcctatcca gcaagcaaaa caaccccttc cattttttca aagtcatcaa
      181 gaaagtagag acgctttttg gcgttctgca aaaaagcaat gtgcttttaa agcagtatcg
      241 aaaaaatata ccgtctattt tactattgct aaaagtagag gagatgttat ttttaataat
      301 ctgttgattg aaggtgaaac ttttgaatgg aaaaaatttt ttcggtatat ggaagcttgc
      361 gctcgttttt ttattaaaga aaactgttgt tttttatttc aatctcctct atcagaagaa
      421 tggttgcgta tttttcataa aaacggctat caaggaacca cacaattaaa taaaaagctg
      481 gtctatcata cagcacttgt tttaggtgga ggaggagcgc acggtgctta tcagattggt
      541 gtgtggcagg cgctgaagga acatgatatc aactttgaga ttatcacagg tacctcggta
      601 ggtgcgttga acggcgcatt gattttacaa ggagatatga aaaaagctgt gaatttatgg
      661 aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa tggctgcagt tgaagacctt
      721 cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa cagcactaat tgaaggcggg
      781 gtttcttctg ctccattgga aaagatggtc aaagacaatt tggatttgtc cttactcaaa
      841 cataatccca aaatacgttt atatacggtg tctactaaac tgccagaact agcggaagta
      901 gtgactgata tccaacagac aaaaacagaa gaaataccag attggatact cgcctctgct
      961 tcattttatc ccgctatggc ttatcgaaag atcggaaaaa ataaatatgc agacggtggg
     1021 tatcgcaaca aaatacctat tgatattgcg attaacaaag gagcaacaga ggcatttgtc
     1081 gtagatgtgc aagggccagg cccagcgaaa aaaattcgta tgccagacat ttttatccat
     1141 tggaaatgtc aaacattgtg gacactaggc agctttctgc tttttgatag ccaaaggaac
     1201 caattaaatt tacaattagg ctatctggaa atgaaaaaac gattaggcta ttactatggg
     1261 aactggtata catttcattc tgttaaacct gcaagaacat actggcgagg atttttgtct
     1321 tacttaacaa aagaaattca gttagaatta tcttttttca aaagcataaa attttggcaa
     1381 aaactgcgta atttatataa gaacagggtc gtaccagaga catgcggatt ggcgatgcta
     1441 gaacttttag caaaaaaaca ttttttactg cccaatgaaa tctatcaagt agacatgatg
     1501 attcaaatga tctgccaaca agatataaaa agtatgccgg atgatttatt ggcacaaatt
     1561 ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat accagcaaaa aatacaaaat
     1621 gagcgcacga aaactgttta ttttgattat ctgctacaag gaaaacgcaa ggatcgtttg
     1681 cagcatgaat tgcttaagca accagttgac acattactga tcctatattt atattatctc
     1741 aaggaggaac aaccatggca caagaatttt catatgaaat cgtagaagaa atcgctatat
     1801 tgtcagaaaa caacaagggg tggcgtaaag aactgaacct agtcagctgg aatggtcgcc
     1861 cgccaaaatt cgatcttcga gattgggcgc cagaccatga aaaaatgggg aaaggcctga
     1921 cattgacgaa cgaagagttt gaacaactgc aaaaagcaat agaaaatatg taatgaggaa
     1981 ggaccctgtc gaatgaaaag tatgactggt tttggcaaag ccatccgcga aacaaaagcg
     2041 tatcagttag atattgaaat aaaaagtgtc aaccagcgtt ttctagatgt tcaaatacga
     2101 agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc agcttattaa acagcagtta
     2161 agccgcggtc gaatcgaagt atttgtgaat ctagcttata ttggcaaaaa cgaaaaacag
     2221 atcgtcatca actgggaatt gattgattca cttatcactc aattgacaga cggaattcaa
     2281 caacgatacg gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg cttcgtgctg
     2341 gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga attggcacag
     2401 ttagctctag atacattgaa aagcgcactt gattctattg aggcgagtcg agaaaaagaa
     2461 ggtcaagctt tagctttagt gttgaaaaag aatactgaag aattcaaaga agtactaaaa
     2521 aaactcgata gttttgtaga aatctatgaa aaggaatttc aaataaggta tcaaaaaaag
     2581 ctggaggaat ttcttggagc gaaagtcgat gagcaacggt tgttgacgga gctagccatc
     2641 ttgttggaac gcggagatat tcacgaggaa ctggatcgtc tggcgatcca tgtacaaaaa
     2701 ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg aattagattt cctgatccaa
     2761 gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga aattaaaaat
     2821 caagtcgtcc agtctaagac gatcttagaa aaaatcagag aacaagtaca aaatattgaa
     2881 tgacgaaaga ggtagagata atgaaatatt atgacgtaac gttccacgaa ttaagtggaa
     2941 aaactgtggt taaacgggat atcccttctg aaaaaaacgg ctttgacgta tggaaagatg
     3001 cctgtgcaga ctataatgag aacgaactgt ttattttgat caacgacggt gcttacgtga
     3061 cgatgaatcg gaaatttatt gttcgaattg acacagaaga ggtagaagat ccgacagaaa
     3121 aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc aatatgggct
     3181 tttagcaagg atgctgaaca aagaaataca ataaataaac agaattggat ttcttcgctt
     3241 agcttctttg ctctaggaat acatttaaag gttgtgacaa aattcgtgtc acaatctttt
     3301 tttattttga ataagtgatg ttcaaaaacc attttctcgg tattaagcca aatttatgca
     3361 aaaatgtgag aaactatttt cacaaaattt ttcttcatac tcgaaactaa tcggcttctt
     3421 cacgacctct tatacacggc gttcaaaagc ttgcatctgc gataataacc ccaaccaaac
     3481 gaaaaacatg agaagctgtt tatgtttggc tgttcttatt acttgatgca ggacagcttt
     3541 tttacgacct cttggtacag ggtagctttt ttaccaattt ctagctataa ttaaagataa
     3601 ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa gtacaaaaag actagttgaa
     3661 aggacgtcgt catgtcagag cgtggattat taatcgtgtt gtctggtcca tccggtgtag
     3721 gaaaaggcac agtacgaaaa gcaatttttg agagtgagga taatgatttt cagtattcga
     3781 tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga aggcgttgat tatttcttcc
     3841 gaagcaaaga agagtttgaa tcaatgatcg aagctggaga gatgctggaa tacgcagaat
     3901 atgtcgggaa ttattatgga actcctttgt catatgtcaa caagacattg gatgaaggaa
     3961 aagatgtttt cttggagatt gaagtccaag gagcaaaaca agtcaaagaa aaagtgcccg
     4021 atggtgtatt catctttttg actccgccag atttagcgga attaaaatca cggatcgtag
     4081 gccgaggaac agatgcagat gaagtaatcg aaaaacgaat gaaagttgca aaagaagaaa
     4141 tcgaaatgat ggctctttac gactatgctg ttgtaaatga tgaagtgccg ctagcagttg
     4201 aacgaatcaa aaacattatt gccagtgaac attttcgagt agatcgcgta atcggtaaat
     4261 atattaaaat gttaaaggag atgtagtctt atgatgttaa aaccttcaat tgactcacta
     4321 ttagatcgtg taaattcaaa atattctcta gtgattttag caagtaaacg cgcgcatgaa
     4381 ttggatgcaa gtgcacagcc tactctagat tcttttgatt cagtaaaaag cgtcggtcaa
     4441 gctttagaag aaatcgatgc aggaaatgta gtcaatgatc cacatcctga attaaaacgt
     4501 gaacgtttga agatggaaga agaggaacgt caagcacaaa aagagcgtga acaacacgaa
     4561 ttggaatcac gtatccgtga agagcaaaaa atgtaataaa gaaaaataga gcgaacatct
     4621 aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt cgccacttat tgtctggtag
     4681 aaaaggctgt gtctgaactt tttgacacag ttcttttttt tcttatctgc tgttaatttt
     4741 ttgttaggat ttggcagaag ataagacaaa tccatcgatc tctagtaaaa taggcaagga
     4801 actttttgtc gcttgataag aaaagacaaa aaaaggagga aacgaaccat gacattaaca
     4861 gctgaagtca ttgtggacgt acctactatg cagaccgatc agccattcac ttatttggtt
     4921 ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag tagaagtccc ttttggtaat
     4981 ggcaatcgtc atgtccaagg atttgttatg gctatccaaa aatcagaaga atcagctaat
     5041 ccatctttga aggcagttat tcgtttgcta gaccttgctc cggtggtcaa tgaagaatta
     5101 ctttcattag ctgattatat gaaaaaaatt acttacgcat tcaaaatcac ttgtctacaa
     5161 acgatgcttc cgagtgtgat gaaagcagaa tatgataaat tgatctatcc tttggcagac
     5221 acaccagaag ttcaggtatt gtttgatcag cgagaagtga tttcctggaa ggaagcagaa
     5281 gaagaaggca gcctttctca attgattcgc tggcgccaag agcaattagt agatatcaaa
     5341 tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt tggtccgttc tttgttaact
     5401 gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa atgccaagaa acaaaaagaa
     5461 cttcttttgt gtttatctga actgagccaa gaagaaccaa ttgcttattt caaaaataaa
     5521 ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg gctggctgga gtttatagaa
     5581 tcagaacgtt atcgcgaccc ttataaagac cgagtatttg accaaacgac ggcattggaa
     5641 ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca cagcaggtca gcagcaaaaa
     5701 gatcaggtct ttttgctgga gggaatcact ggcagcggga aaacagaagt atatcttcag
     5761 gcaatcgctg atgttttgtc agaaaataaa acggctatta tgttagtgcc tgaaatcgca
     5821 ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg gagaatcagt tgcggtctta
     5881 catagcggac tttcccaagg tgaaaaatat gatgaatggc gcaagattga gcgggaagaa
     5941 gcgcaagtag tagtaggcgc acgatctgct atttttgccc cgctgaaaaa tatcgggctg
     6001 atcattatcg atgaagaaca tgaatcaagt tacaaacaag atgagacacc gcgttatcat
     6061 gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt gcccaatagt tctaggaagc
     6121 gcgacgccct ctttagaatc aagagcacga gctcaaaaag gcgtttatca gttattgcag
     6181 ttaaaccacc gagccaaagc agctgcacag cttccagcta ttgagatcgt agatatgcgt
     6241 gaagagtttc agaatcatcg gacctctact ttttctgcta atttgcagga aaaaatacaa
     6301 aatcgattag acaaaaaaga acaaactgtc ctactgctca atcgtcgagg atattcgtct
     6361 tttgtgatgt gtagggactg tggatttgtt cttccatgtc ctaattgcga tatctctctt
     6421 acactgcata tggatacacg ttcaatgcgc tgccattatt gcggacatga agaacctatt
     6481 cccaatcgct gtcctaattg tggcggcaat aaaatccgct actatggaac aggtactcaa
     6541 aaagtagaag aggaactccg agaactttat ccgcaagcta ggattttgcg tatggatgta
     6601 gataccactc gaagaaaagg ggcacacgaa cagattttac aaaaattcgg tgcaaaagaa
     6661 gcagatatat tattagggac acaaatgatt gctaaaggtt tggactttcc agaagtgaca
     6721 ttagttgggg tgttgaatgc tgatacctcc ctgaatttac cggattttcg ttctagtgag
     6781 catacattcc aattgcttac ccaagtttca ggacgagcag ggcgagcgga aaaagcaggc
     6841 gaagtcgtga tccaaacatt caatcctcaa cattacgcca tagaattggc taaaaaacaa
     6901 aattatgagc agttttatca gcaggaaatg catgtacggc atcgtggagg ctatcctcct
     6961 tattacttta ctgtgaaaat aaccgccagc catcctgaag aacaagtagc ggcaaagaaa
     7021 atctttcaga ttgccaatca gctaaaagaa gtgttaagcc ctcaaagctt gctattaggg
     7081 ccaacgccca gcatgatttt gagagtgaaa aaccgatatt attatcaact aatcatcaaa
     7141 tataaacatg aaccgaatct tcctcatgtt ttggatgaaa ttttgaatgg aagccagaaa
     7201 gaacagcgac aaggtctttt tgtggcaatt gacaacgaac cattaaactt tatttaagaa
     7261 agggccaaat tatgcgctat cctatattga tccaccctaa tgataaatta aaaagaactg
     7321 ctcagccaat cgatgtaatc acggacgaga caatcgcttt attagacaat ttatatgaga
     7381 caatgattgc gaatgacgga atagggatcg ctgctcctca agtaggacaa aacaagagaa
     7441 ttgctgtgat cgaagtagat gaaggcgaga aatttgaact gattaaccca gaaatcatcg
     7501 aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag tattccgcat gtctatggaa
     7561 cggtcaagcg agcagatgaa gtgaccgtgc gttattatga tcgagacgga gaagagatag
     7621 aagtgacggc ttttggctat ttagcccgtg ccttccagca tgaaatcgat cacttagacg
     7681 gtatcttgtt cattgagaaa atgatacagc agatcccgga agaagaactt gaagaatata
     7741 tggaggaaca tcttgatgac taagctagta tttatgggca cacctgcttt ttctgtgccg
     7801 attcttgaag ggctgataga atcaggttac gagatccagg cagtggtaac acagcccgac
     7861 cgtccagttg ggcggaaacg agtaattacg ccgacaccgg taaaaaaagc ggctctgaag
     7921 cacggtatcc gtgtgctgca gccagagaaa atctccggtt cgccagaaat ggaagaaatc
     7981 attgaattgg tgcctgatgt gatcgtcact gcggcatttg gccaattctt gcctgaaaag
     8041 ttattgcaag taccaaaatt aggggcaatc aatgtccatg cttctttact tcctaaatat
     8101 cggggaggtg cgcctgttca ttatgccatc atgaatgggg agaaagaaac tggtgtaacg
     8161 atcatggaaa tgatcaaaaa aatggatgca ggcggaatct atgcgcaaga aagcatacca
     8221 atcactaaac aagatgatgt agggacgatg tttgaaaagc ttagcctttt aggaagaaag
     8281 ctattgctag aaacattgcc caatattttg gatggacaaa aaccagtacc tcaagacgag
     8341 tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag aagcaatcga ttggcataaa
     8401 actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc cttggccaat tgcattcacg
     8461 acctatgagg gcatacgctg gaaattatta tcggttgaag tattggcaga aactacagac
     8521 aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc tatggattgc ttgcggtaaa
     8581 cagaccgtct tagccatcaa tgaattacag ccagctggta aaggaaaaca ggccgtacat
     8641 gatttcctta acggaagcgg tcagcaagtt cagattggtc agcaggtgaa ataatgaaaa
     8701 aaatccctaa aaaaatgaaa aaatcagtac gatatatggc attactgaca ttagagcgag
     8761 tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga gatgatcaaa caaagcgagc
     8821 taaatcaaaa agatgtgggt ttatttacag aattgatcta tggtacgatt agccatcagc
     8881 gactattgga attttacgtt gcaccattga tcgctaaagc aaaaaaagtg gatgactggg
     8941 tcaagatgct tctttatctt tctgtatacc aattagaatt tttagataaa gtccctgcac
     9001 atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa aggaaatcct gggacaggga
     9061 aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg agcaccagat ctgcaaggca
     9121 ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct gcctctatgg atgacgaaac
     9181 gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct cggtttatcg ctatatcagc
     9241 ctagccatgc tagtgcccga gtagatacac gccgactttc cagagatcag gccatcactg
     9301 ttttgcaaga agaagagatc acagctgaaa aaagccaagt cagtccatac ggtatagtag
     9361 cgaaaaaagg gcatttagct tctagctcat tgttccacga tggcgtcatg acgatccaag
     9421 atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga gcctgaacat catgtcttag
     9481 atgcctgtgc agcacccgga ggtaagacga cgcatattgc ttctttttta gatccgtcat
     9541 gcggtggaga agtggtcgct ttagatgtac acgaacacaa agtcaaactc attgaagata
     9601 atgcagcacg tctaggtgtc tcttctgctg tctacgctca aaagatggat gctagagaag
     9661 tcagtaaaca gttcgaagca gagcagtttg atcggatttt agtagacgcg ccttgttcag
     9721 gtcttgggct gcttcggcga aaaccagata ttcgttataa aaaacagccg gatgaattag
     9781 cgaatttacc aaaaatccaa ttggaaattt tagaaagctg cgcaccaact ttaaagtctt
     9841 ccggtattct gacttatagc acttgtacga tcttaaaaga agaaaaccaa gacgttgttt
     9901 ctgctttctt ggaaagacac cctgattttg agaggattga tgttttgacc aatcctatgc
     9961 tacaatcatc aatcgaagag aaaatgttga ctttgtatcc gcatcagttt tacacagatg
    10021 gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga aaaaatgcag attgaatatc
    10081 aatcagatgt aggaagacga cgtaacacaa atcaagacta tgccagtgta tttaccagcc
    10141 gttcaggtct caagcttgct gtattagcag acgggatggg tggtcatcgt gccggagaca
    10201 ttgccagtca gatggcggta acgaatctag gcgaagcttg ggaaaaacaa gaagctgttg
    10261 acgatgaaaa aatcgcccaa tggttcattc aatcgattca agaagaaaat atgctgattt
    10321 atcagcgtgg acaagaacag ccggaatata acggtatggg aacaacaatc gtagcggccg
    10381 ctctgtcaga tgactggttc acgattgcca atgttgggga tagcagagct tatttgattc
    10441 gtgaagataa aatcatgcaa ttgacggagg atcattcttt agtcaatgaa ctagtcaaat
    10501 caggagaaat cagcgaagaa atggcagtga accatccaag gaaaaatatt ttaacacgtt
    10561 ctgtcggcat gcctggaaca gtcgaagtag atgtttctac tcatctttgg caactaaaag
    10621 atcgactgct gttatgttca gatggcttga cgaacatgct gtctgaagaa gaaataggaa
    10681 cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac tgagctgatt gatcgtgcca
    10741 atgaagcagg cggagcggat aatatcaccg ttcttcttat cgaatataag gaggacagtg
    10801 tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa atcactggaa atatcggtag
    10861 cggtggtatg gcaaatgtat ttttggcgca cgatttaatc ttggatcgtg atgtagcagt
    10921 taaagtctta cgatttgact ttcaaaatga ccagactgcg attcgacgat ttcagagaga
    10981 agctttagca gcaacagaac tcgttcatcc aaacattgtt agtgtttacg atgttggtga
    11041 agaagacaac atgcagtatc tggtgatgga atacgtcaaa ggaatggact tgaaacgata
    11101 tatccaaaca cattatcctg ttccttatga aacagcagta aatattatgc agcagatcct
    11161 ttctgctatt tctttagctc ataaccatca aatcatccac cgagatttga aaccccaaaa
    11221 cgtactgatc gataatgaag gtgttgtaaa aatcacggat ttcgggattg ctatcgcttt
    11281 atctgaaaca tcgatcacgc agacgaatac gatgctagga tctgttcatt atctgtcccc
    11341 tgagcaggcc agaggaagta tggcaacgaa gcagtcagat atttatgctt tagggatcat
    11401 cctttatgag atgttgacgg gtagcgtacc gtttgatggt gaatctgctg taacaattgc
    11461 tttaaaacat tttcaagatg atcttccatc tataaaagca cttgatccaa atgtgccaca
    11521 agcactggaa aacgtgattt tacgggcaac ggctaaagaa cctgcagacc gttataagtc
    11581 agccgaggaa atgtctgatg atctttctac agtattatca ccagctcgtg caaacgaaga
    11641 aaaatggcag cctcatgtga tggataatga aacaaaagtt atcacacccc ttacggaaga
    11701 tgcaccaatg cctgattcat tcaaatctat gcctctgcca aaagacaagc cagaaaacga
    11761 aaccttacca gaggaggaag aagttctgcc tgatcctaaa aagaaaggac agaaaaaatg
    11821 gtggctgatc ctattagcta ttcttgctgt tttaggaatc ggaacagctg tattctttgc
    11881 ttctggcggg cgaggagaag ttgttgttcc tgatgtttct gatctttctg aagcttctgc
    11941 tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca aaaacagaag aaattgcaga
    12001 tgacaaaatc gaagaaggta atgtagtgaa gacagacccg gcagcgggaa cgacagtcaa
    12061 gcaaaaacgc gaagtcactc tgtatattag tagcggcacg aaaaaaatca aattagatga
    12121 ttacagtggt aaaagttatg aagatgccga agcaagtttg aaagaattag ggttttctgc
    12181 tgacttgatt caaaaagaag aggaattcag ttcagaggta gaaagcggaa tgatcatttc
    12241 gcaatctcca tcagaagata cagaagtcga tcctaaaaca gacacgatta ctttcacagt
    12301 cagcaaagga ccagaaacag tgactttgag tagtcatgta ggagaaaact acgaaactgt
    12361 tcgtaattgg cttgttacac aaggattcaa tatcaataat attagtgcta cttatgacta
    12421 tagcgacact gtaccatcag gttcgatcat cagtcaggat ccagcctcgg gtgaagttgt
    12481 agcagaagaa acttacatca attttgtcgt aagccaagga acagagccta aattgaatga
    12541 tatctccggc tatacaaaaa gcgaagcaca aagttattta gctagtgtcg gtgcagaata
    12601 tatcgggcat gagacttccg aatattcaaa cacggtggat aaagataaag tgattcgtac
    12661 agtaccaggg gcaggaacga caattaccaa aggaatggtt gtgaacgtaa tttactctaa
    12721 aggaccagat ccagcgtctt ctagttctga aaaagactct tcagacagtt ctagcagctc
    12781 aagtagctcc agtagcacaa gcaactcttc aaaagaagaa tcttccagct ccacaagttc
    12841 atcagctagc acctcagaaa gttcaaaata agtgctgaat aaaatatatc agaagggaat
    12901 gcgacattga tttgttgcat tctctttttt gtgtttctat ctcttttgat caattttcaa
    12961 gccatatact ttgagaagtt tgcatggaat aacacctact ttcctcttcg tatggtaaaa
    13021 taaacaaaaa cggaggtggc acttatcaaa ggacaaatca gaaaagcaat cagcggtttt
    13081 tactatgtat acgcaaacgg agagacctat caaacaagag gaagaggaaa tttccggaat
    13141 cggaaaatca cgccgcttgt cggagatcag gtgatctttg agagtgaaaa tcaaacagac
    13201 ggctatcttc tggaaatctt gccgagaaaa aatcaattag ttcgcccacc agtcacaaat
    13261 gttgatcttg gaatcattgt cacaagtatc gtagagcctg cattttctta taatctactt
    13321 gaccgctttc tagttacctt ggaatacgag tcgatcgaac cgattatttt tttgacaaag
    13381 accgacttgt cagacgaccc agaaaaaatc gaagagatca agcaaaccta tactaaaata
    13441 ggctatccgg tgattgtacc caatcatacg ggtgatacga cagaactgat ccgttacttc
    13501 ccagaacggc tatcggtctt tatgggtcag tctggagcag gaaaatcgac gctactgaat
    13561 caaatatcac cggaactgaa tttagcaaca ggagagattt ctgatgcact tggaagagga
    13621 cggcatacaa cacgtcatgt ggaactgatt cctatctatg gcgggttagt agcagataca
    13681 ccaggattta gctcgattga ctttttgaca attgaagcag gagaactgcc gagacaattt
    13741 cctgaatttg tagaagcttc tgtccattgc aaatttagag agtgcatgca tgctaaagaa
    13801 cctggctgcg aagtaaaaca tagagtagaa acaggtggta tcgcacagac acgttacgac
    13861 aattatttgc aattcttaca agaaatcgaa aaaagaaaac caatgtatgc aaaaaaagaa
    13921 ggtaaacgaa aataggagga aacaagaatg aagatagcac catccatttt gagtgcagat
    13981 tttgccaatt tacaaagaga cattgagtta gtggaaaaag gaggcgcaga ttacatccac
    14041 gtagatgtaa tggatggccg atttgtccca aatatcacat tagggccaaa tattgttcag
    14101 gcaatccgtc ctgttactaa attaccgtta gacgtccatc tgatgatcgt tgatccagaa
    14161 aactacattc cagcatttgc aaaagctgga gcagatatca ttactgtcca cgtcgaagca
    14221 actcctcata ttcatcgggc acttcaaatg acaaaaagct taggagttaa agctggagta
    14281 gttatcaatc caggaacacc agtctccatg atcaaacatg ttttgccttt ggctgatcaa
    14341 gtactagtca tgactgtcaa tcctgggttc ggcggacaaa gctttattga agaaacagta
    14401 gaaaaaatta cggaactcgc tgatctacgt agagagaaca attggactta tgcaatcgaa
    14461 gtagatggcg gaatcgttcc agaaacagca aaaatctgta aagatgcagg ggcggatgtc
    14521 ttcgttgcag gatcatatat ctatggagcc gaaaatccag tcggacaaat cgaacgatta
    14581 aaagaggcat tagactaaat gaaaatttta ttagctgctg gaggtaatcc gcaaaactgg
    14641 ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga tagaccgtgg tgcgctgtac
    14701 ctgcttcaag caggctaccc agtagatctg gcagtgggtg attttgattc attgtcgaac
    14761 gaagagcaga aggaagtatt tgcaaaagcc aaagagtata cccgttcccc tgcagaaaaa
    14821 gacgatacag atacacagtt aggtatctta aaaacatttg aacgttaccc gcaagcggaa
    14881 gtcactttga ttggagctac gggcggaaga ttggatcatc ttttagccaa tctctggtta
    14941 ggtatggaac ctcgcttcaa accctttgcc catcagctta ttatcagaga ttgtcaaaat
    15001 tatttgtctt attatttgcc tggagagcat atagtaagac agataccaga tatgaagtat
    15061 ttagcttatt gctgcttgac tcccgtctcg aatcttacat taaaaggcag caagtatgta
    15121 ttggaaaata aagaagtaga atatccgaca tcttatgcaa gtaacgaatt tctttcagat
    15181 accgcagaat tttctttttc agatggtatg attgcagtta ttcaaagcaa agattgatcc
    15241 ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga cagaatctat tatttgctaa
    15301 aaaaaagctt aagaggagca acttaccttt tgtttcacgg catttagtga taaaattgaa
    15361 cacaagagtc taattaattc gaggtgtctt tgtgaagaag ttcaatccta ataaaaatat
    15421 tattattacc ttggttattg ttatcatcgt ggtgacgata ctaagtgtga ccgctgcgca
    15481 gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc gttaatgaca gtgttgcaat
    15541 ggttgataaa acaatctctg ccccagtacg gtgggtacga aatggagtag attctgtcca
    15601 taatcttttt tcaacttatg ccgaaaatga gcggctaaaa gaaaaaatcg atagctatga
    15661 tgacgtttca cagcaaaaca agaattacga gagagaaatc gaagcgttga aaaaagaact
    15721 tgagttgaat cagacgctga ctagttacga aaaagtaacg gcaaatgtca ttactcgttc
    15781 gcctgacagt tggcaggaca tgctgatcgt agacaagggt tctaaagacg gtatcgaagc
    15841 aaatatggca gtcatgtcgc aaaaaggatt gattggccga gtgatcgagg tcaatacggc
    15901 ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc tccaatcatt ttccagtacg
    15961 ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa aactatgatg aaaagctcca
    16021 tgccttagtg gtgacccaat taactggtga tacggatatc aaagaagggg atgttgtcca
    16081 gacatccggt cttggaggga attctccagc taacttgccg atcggtacgg ttattaaaac
    16141 gaaaccagat agttatgggc tggatcggga agtttatgtg aaaccttatg cagaaatgta
    16201 tgacgtgtca gttgtgacga ttgtccaacg gctagcggag gattagtcca tgttgaagaa
    16261 agaaacaatg aagtactatc tgccaatcgt tttgttcttt ttgatgttga tagatggtca
    16321 tttaacaaga atgctagggg agtggtcgaa aggcacctat atgtcaaatg cccactttct
    16381 gatattggca ttattatgtt gcagtatggc gtttgaaaaa cgttatttac tgattaccac
    16441 gattgttctc ggggctatct atgacgctta ctatattggc gttatcggta tctatgcagt
    16501 agctctccct ttaattgtat ggttgatgta tgtaatgaaa gacgttatcc atgtcaacat
    16561 ctttactgaa tttttcagta tgatcatctt tgtcacgggt tatgaattgt ttacgatggt
    16621 ggtccagttg atttttaaat tagcagtagt aaataacacg tattttatta caaggttttt
    16681 aggacctaca ctgctgttga acatgattat atttgtatta ttcatttttc cctttaagaa
    16741 attattcagt gatgaataat ttcttttttt atttttaggt cgaaagcgaa aaagaatcgt
    16801 tgttatctta agaaatgcta gacttaacgc ggctttttca tgatcatttt ctgaaacgaa
    16861 cagttttgtc atttttaaaa gttgttttga aacatttttg taacataact attatatcgt
    16921 tgacacactg ctatggtaca atttttatat cgttaagaaa gacttaaata tatttagata
    16981 tttattaaat taagccagtc ggaggaatga agagtgaaaa agagtttaat atcagcagta
    17041 atggtatgtt caatgacctt gactgccgta gcatcaccga tcgctgccgc ggcggacgat
    17101 tttgattctc agatacaaca gcaagatcaa aagattgcag acttgaaaaa tcaacaagca
    17161 gatgcacaat cacaaatcga tgccttagaa agccaagttt ctgaaatcaa cacacaagca
    17221 caagacctat tagcaaaaca agatacattg cgtcaagaat ctgcacaatt agtaaaagac
    17281 attgcagatt tacaagaacg tattgaaaaa cgtgaagata caatccaaaa acaagcacgc
    17341 gaagcacaag taagcaacac aagctcaaac tacatcgatg ctgtattgaa tgctgattca
    17401 ttagcagatg ctattggacg cgttcaagca atgacaacga tggtaaaagc aaacaacgac
    17461 ttgatggaac aacaaaaaca agataaaaaa gctgtagaag acaaaaaagc tgaaaacgat
    17521 gctaaactaa aagaattagc tgaaaaccaa gctgctttgg aatctcaaaa aggcgatctt
    17581 ttatctaaac aagctgattt gaacgtactg aaaacttcat tagctgctga acaagcaact
    17641 gctgaagaca agaaagctga cttgaaccgt caaaaagcag aagctgaagc agagcaagct
    17701 cgtattcgtg aacaacaacg tctagcagaa caagctcgtc aacaagctgc acaagaaaaa
    17761 gctgaaaaag aagcacgcga acaagcagag gctgaagcac aagctactca agcttcatct
    17821 actgcacaaa gctcagcaac agaagaatca tcagctacac aatcttcaat gactgaagaa
    17881 tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa caacacctga atcttcaaca
    17941 gaagaatcaa cagcgcctga atcttcagca acagaagaat caacaacag
//
LOCUS       DAZKOM010000055        17389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000055 DAZKOM010000000
VERSION     DAZKOM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.055"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..633)
                     /locus_tag="ACG8NF_001760"
     CDS             complement(88..633)
                     /locus_tag="ACG8NF_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0472240.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(645..1337)
                     /locus_tag="ACG8NF_001761"
     CDS             complement(645..1337)
                     /locus_tag="ACG8NF_001761"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0472241.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1356..1640)
                     /gene="macP"
                     /locus_tag="ACG8NF_001762"
     CDS             complement(1356..1640)
                     /gene="macP"
                     /locus_tag="ACG8NF_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0472242.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1653..2216)
                     /locus_tag="ACG8NF_001763"
     CDS             complement(1653..2216)
                     /locus_tag="ACG8NF_001763"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0472243.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2444..3106
                     /locus_tag="ACG8NF_001764"
     CDS             2444..3106
                     /locus_tag="ACG8NF_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0472244.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3230..4426
                     /locus_tag="ACG8NF_001765"
     CDS             3230..4426
                     /locus_tag="ACG8NF_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0472245.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4527..5903)
                     /locus_tag="ACG8NF_001766"
     CDS             complement(4527..5903)
                     /locus_tag="ACG8NF_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0472246.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5940..6806)
                     /locus_tag="ACG8NF_001767"
     CDS             complement(5940..6806)
                     /locus_tag="ACG8NF_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0472247.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6895..7920)
                     /locus_tag="ACG8NF_001768"
     CDS             complement(6895..7920)
                     /locus_tag="ACG8NF_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0472248.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7938..8612)
                     /locus_tag="ACG8NF_001769"
     CDS             complement(7938..8612)
                     /locus_tag="ACG8NF_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0472249.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8796..9689)
                     /locus_tag="ACG8NF_001770"
     CDS             complement(8796..9689)
                     /locus_tag="ACG8NF_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0472250.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9763..11460)
                     /locus_tag="ACG8NF_001771"
     CDS             complement(9763..11460)
                     /locus_tag="ACG8NF_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0472251.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11453..12766)
                     /locus_tag="ACG8NF_001772"
     CDS             complement(11453..12766)
                     /locus_tag="ACG8NF_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0472252.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12766..16707)
                     /locus_tag="ACG8NF_001773"
     CDS             complement(12766..16707)
                     /locus_tag="ACG8NF_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0472253.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16816..17274)
                     /locus_tag="ACG8NF_001774"
     CDS             complement(16816..17274)
                     /locus_tag="ACG8NF_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0472254.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4876 a         3902 c         3019 g         5592 t
ORIGIN      
        1 taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta ttttgtgctt
       61 tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg tggactcaaa
      121 atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggaat ctttgaaaat
      181 atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac agatatctgt
      241 acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga tgtctagatc
      301 tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt cctcttgctc
      361 atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg ttcctatcac
      421 attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat catgcgcatc
      481 aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag cagtcagctt
      541 tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg caacaaaatc
      601 atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac gagatgtttg
      661 acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag
      721 ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata
      781 ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc taatgcttca
      841 gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt atcacctgta
      901 acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac catttcactg
      961 cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc tgggttgtag
     1021 ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac gatgtcacct
     1081 acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat gttcacgccg
     1141 tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat accggaacgt
     1201 acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc
     1261 catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc cattgcgccg
     1321 ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat tgctaaaaaa
     1381 accacacata acattaggat aacaataata agagatttca tcaaaaaaga gttccatttg
     1441 gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt tttgctttct
     1501 ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc tgcttttcgc
     1561 tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta
     1621 ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa tagttcccag
     1681 tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat cgcttgttgc
     1741 gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg tgctaaagga
     1801 ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct
     1861 ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc agtttcttct
     1921 tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc gatttttcca
     1981 gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac aagaagcaga
     2041 cgatcttgtt cgtcaaaagc aatgatcccg acaccgccag gatgaaatac cagttctctc
     2101 ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat aatttttccg
     2161 ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt cagcatatct
     2221 tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt ttctcaaaaa
     2281 ctccttatta gtttaccata gataaacctt agattttctc taaatataga aaatccttct
     2341 ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat cgaataacga
     2401 aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa aacgaaattg
     2461 gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata gtttttcttt
     2521 tgttctagga cttttattga taggtgtagt tttagttttg tggggactct ttggttcgcg
     2581 caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg aaactcacta
     2641 tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac
     2701 aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat
     2761 cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga
     2821 accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga atctagttga
     2881 tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac agacctatga
     2941 aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg cacaagatta
     3001 tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg ttgcgaaaca
     3061 gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct tttgttcata
     3121 aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt tctaaagtag
     3181 agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta tggatcataa
     3241 accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt tattgaacaa
     3301 cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga ttgatcagct
     3361 ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac gccaagaaca
     3421 agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca tcagctacgg
     3481 atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc
     3541 acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc tccaagaagc
     3601 caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt
     3661 caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac aaattgacaa
     3721 gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc tgatgttaga
     3781 acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata tcgctgctgg
     3841 agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga aaagagcaga
     3901 agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat tcctagatcg
     3961 tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc agcaagcacc
     4021 acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc aagcatctat
     4081 cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca
     4141 aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact tattgaagaa
     4201 aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg aacgtggaat
     4261 cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa tccaagagac
     4321 attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca
     4381 catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca cctttcgctt
     4441 gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg tttttcttat
     4501 ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg tccatagcca
     4561 tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat ccatgtcaca
     4621 gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg caatagcgga
     4681 acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct caacttcagt
     4741 aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag ttccgctaat
     4801 acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc ataagtcgct
     4861 tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag taagccatcc
     4921 ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt taccgtcagt
     4981 agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc tacatacctc
     5041 attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat tcctgtcaag
     5101 atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat gattcctctt
     5161 ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc atcaaatccc
     5221 aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc aaaaggcatg
     5281 aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa caacgcgatg
     5341 ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt ggcttcaagc
     5401 gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat gtatgtacca
     5461 ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa acttttcaaa
     5521 aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag taaaaattcc
     5581 ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac atacagatag
     5641 gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact tagtccagac
     5701 aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc agcattttga
     5761 ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc aattccaagc
     5821 atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt gagattgatc
     5881 tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga aaacagcgat
     5941 taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag gcaaccaaca
     6001 ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa
     6061 gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg
     6121 actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat catgtttttc
     6181 cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc taagatcatt
     6241 ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt gacgatgatg
     6301 gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt accaaaatca
     6361 agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt caaatacgca
     6421 ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt ttgtgtccat
     6481 tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc agtagacaat
     6541 ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt ttgtccaaca
     6601 ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat tacaggacta
     6661 acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt
     6721 tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca tgccaacata
     6781 gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat gataagaaac
     6841 ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga ttttctaacg
     6901 tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc cttttccttc
     6961 agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg ctaaataaag
     7021 tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa atcccttatc
     7081 aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa ctgaaaggct
     7141 gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg gcgtatattt
     7201 gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat cggtcaaaac
     7261 agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat aatttgcctg
     7321 actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga ggtagtcccg
     7381 cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat cgatttttga
     7441 taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga tcgaacgaac
     7501 tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt caatataatc
     7561 tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt cacggatcag
     7621 ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac tcaaataatt
     7681 ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc tcttgctcaa
     7741 gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta aataagcaat
     7801 tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg tcaatgcaat
     7861 aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa aaaaattcat
     7921 cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt ttcgatatat
     7981 ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta
     8041 ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc ccgacttaag
     8101 atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt gctcaaatca
     8161 atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat gccattgtgg
     8221 ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt
     8281 tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc
     8341 atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat cggtactttc
     8401 gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag attaatatct
     8461 agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt gaaatcggtc
     8521 gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg tcgaatgatc
     8581 tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt tcttcttgct
     8641 actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa tgtcatttta
     8701 gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct tccaagcagc
     8761 ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt ctttcgttgt
     8821 ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac tagcaatata
     8881 cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc tatctttgaa
     8941 gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg agtgttcacg
     9001 gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat tgctaatgta
     9061 tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag catccgacca
     9121 tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt aaagagtttt
     9181 ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc tccagttgga
     9241 aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt cgtttcatca
     9301 aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat atgttgttcg
     9361 taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg atctttgact
     9421 tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt ttcatgcact
     9481 gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg ataattctcg
     9541 atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata tgtaaaaact
     9601 gcagctccgg caagaaacat cactgccata aataattgta ataaaagact gatttgggtt
     9661 tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa aaaaactgca
     9721 gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt cagacttcat
     9781 tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga ttagtaagaa
     9841 tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat tcggtactct
     9901 ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc ttccataaga
     9961 gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt ctggcgccat
    10021 ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt atccatttcc
    10081 atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat ctgctcctga
    10141 ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag aacggtcttc
    10201 attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat catatttttc
    10261 aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt cataatcaaa
    10321 ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat ccgcttctag
    10381 ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg catctgctaa
    10441 tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg tcacgacaaa
    10501 cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg tctcgccggc
    10561 tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat ctgtgatttt
    10621 gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat aaggaatagg
    10681 tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag aaatcgtttc
    10741 ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct ctacattcat
    10801 ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg tattgtatgt
    10861 gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca tcactggcat
    10921 cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag tttctaagaa
    10981 aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg tagagaatac
    11041 cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa
    11101 ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat taagaccgtc
    11161 ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt ccgttacgtc
    11221 aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag aaaccaatcc
    11281 gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg taatcgtcac
    11341 ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc cgaaaatccc
    11401 tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt ttttattcat
    11461 ttttttctcc ccgttctttt tttattaata aaacagcacc taacaataaa agaattccta
    11521 gtaaagaaat caacgttctt gcttcattcg tttgcgggaa tacggaccat tttcctgatt
    11581 gacctggatt gcttggacca ctcggctctt tcggtttgtc cgttgtttct tttacggtct
    11641 cgttataaac acgtggtgtt gcggattcat aggcagagac tggaactttc ccggattcta
    11701 attcagccat ctcctgtccg ttaactacta catattgtgg ttggcctttt gcatccaccc
    11761 aaaattcagg gatgatgacc tcgattctct tacttgcttc atccatctcg taaccttcca
    11821 caccttttaa ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa
    11881 caaggccctc tttatccgaa atgaatttgc ttacattttt gtcattcaaa ggatcattgt
    11941 ctgctggttt gatccattga tttttcaagt cattagcagg agccatatct aaataatatt
    12001 tccccccttc tatcatctga tatagagcaa aaacagcacc ttctaatggt tttccttttt
    12061 cagttgttcc tctttgacgt ccatacttga agaagtatgg atctcttaga taaccgacat
    12121 ttttaggata gatatgaatc tcttgcagtt cctcggttga ttctgtcggg tgataaattg
    12181 gcaaaatagc agcaattggg atagctgtct gatcgatatc aacatccaat ccaacttctg
    12241 gatcaatcga ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt ttcttaggtg
    12301 ctttgatacg tgcaatcccg tctcctaatc cgccaaccgt atctttccca gttgttaccg
    12361 aagtccctat ttggacaagc ctttcttctg tgattagctg acggatattg tttctgttcg
    12421 tttcagcgat ttgctggacg aattctttag gagtcatgtt ttcagcagct tttttttcat
    12481 gataataatc ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc accgtctgtg
    12541 aaatgatatc tgctgtttcc cctgcttcct cagagatagc caagccatca ttttcatggt
    12601 attcaaattg atcgttataa tcgatatctc gaatcatccg tttatgcaac acgaattcca
    12661 cgacatcttc ccctgattct tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg
    12721 gtagtaaaac aagtaattga agcatcaaac acaacagctt tttcattacc ttgctccctc
    12781 ctttttagat tgtctgacaa acaaaccata ggctgctcct gcaagcgcca tgaagaatgc
    12841 agcaagcagg atatagaata atcgtccgga tcctcctgta gcaggcaacg gattttcagg
    12901 atcgttcccg acactaagct gtaatctgat tcgatttgtc tccaaaagtt catattctaa
    12961 ctcttgctgt agatcaggcg tctctgcttc ttcgtttgga taatacgaaa tctcaacttt
    13021 tagtgtaccc gcttcttttc ctggaactac ccgatcattt tctatgtcag gttcttctcg
    13081 ataactaatt tctaaaataa agtaacctgg aagttttcta tagctgtcag gtgacttcac
    13141 ttccttgatc cgatagaccc cttccaacaa gtctgaagca ttgaataggg aagaatcgtt
    13201 tggatctggt gccaagcctc cctcgatgat ttcatactct cctgcactat tttgtttctc
    13261 aattgaaaat tctgcgcctt caagacgtcg gctattcata ctgtcaattt tcaaaagttc
    13321 caaatcgatt ggcgctagtt catcttctaa ggttacttgt agtacaccat cgatgatttc
    13381 atagccttcc ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt
    13441 accttcttca ttcagtgcaa actggaacac ttgatcagat agccgatacc catttggtgc
    13501 gacagattcc gagacctcat attcaccaat agacagatgc agaggttggt cgtaatttaa
    13561 atcgacatac ttttctactc ctacggcacg agaatagaag tatccgatcc caggcaaacg
    13621 tttcgattct tctggttcta acggattacc agtgtatgct cgatacagcg ggaaggaaga
    13681 atcccccact ttcctgatat cgaaacctgc accatctaat gtatggcctt catggtctaa
    13741 ttttctaact cggatctccc gataatcaaa cgtattgtaa gcagtgaatg ctacccataa
    13801 acgatcctct tcatctgttg tatacgtgag atattgccaa ttttcttcac ctgtttgctt
    13861 gagttggata gtccctatag cattatcgta aagtcgcaag atccatgtgt cattttcatc
    13921 aggttctttg aaaccttctg ggttctggct ctcaatgatt ttgtattctg catagttggt
    13981 agtttcttca ctatccttat aaacaaatga atctggctct ccgattggat gaataacttt
    14041 ctcaccatcc acgattacat atttcccgtc ttcatctact gaatagaacg tgagtctccc
    14101 tttctcttcc tctacttcgt tcacaccaga aagccctttc gctactggat atccgatttc
    14161 cagtccgtta ccatctgttt cataaagttc aaactctgcg ccattgattg gcatattact
    14221 gccttcaatt agtttattga tattcatgtg gattggcttg aattcatttc gcatttccag
    14281 tttgacatac aactcatcat actctgtgcc ttctatgtta ggctcttgta caacagaaag
    14341 cttgtgatat tctccttcat aataggaatt cggatcttta ccttccggtc cgccgactct
    14401 cgaaccagat gtatattcat ctacaagctc gtagtgacct aacatctcat cttcgttggc
    14461 tgcatatttt ttgatttgga attgatacgt ctcatctact aactgataac gtggtggtcc
    14521 agcgatctct ctgatccaaa atatgtcgct gactgctcca gtttcatctc ctaaaatcgg
    14581 catcttcgtt tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt cgattggttc
    14641 ggcttcccca ctctctggat tttggtcata taatgcaaat tcgactccag ccaacggctc
    14701 gccattttga cccttcttgc tgacttggaa attacggcct ttatgttcgt aacgaatgga
    14761 agaagatggg aaatacatcc gtccagtagt gatatcattc tcataatcct ccaggttctg
    14821 aatataggcc atattgttca ctgtgcaaaa atttccgtct tgagctcccg catcaagttt
    14881 tacttggtac ttgatcctga ccatttcccc ttcacctaaa gaaatcggag aaaatttata
    14941 agcttggttt tcccgtccga catacgttgt cggagtcgcc actacttctg gtggggcttg
    15001 tccatgacgt tcttcatccc atatttctgg ctcttggtac ttcatgccgt tttctcctgg
    15061 atctagatgg taaaactgaa tatctgccga tctgccagct ccaaatggtg agacattaaa
    15121 accagtttca tcataaagtg tggcttgctg gatcgttctc ttgaaggtag aagagatatc
    15181 ctccaaggct gtaacaatgt cattcttgtt gttcgcattg taatatcgcg gtgttacgcc
    15241 atcttcttct aaatcagcaa tatttttcaa tacatttcgt ccaatagcat ctagtcgttg
    15301 tctcccgcta acgtttccat cgattcctaa accaatggag aagatacttg gcgggttctc
    15361 ttgatacttg tttttcagcc aatgatgata agcaactgtg ttgatcgcag atgacttttc
    15421 ttgcgaagta ggactgccat tcgtattgtt attagaagaa ccatcaccaa cccagtgagt
    15481 cgctttatta tctccttctt ttacctctcc ataacgatag tgtacattta cttcatcttc
    15541 tggacggtta aagtcagatg cataggtaac tggatgagta aaccctgcat tagaagtatt
    15601 cccagaaaac tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc
    15661 catcacagtc cagttgctcc aagctccatt agtgctcgta cgataacttg actgaatcgg
    15721 tgcataactg aaggtcggtg tcccatctcc cacaacgatt aggatttttt ctggatttct
    15781 attttctccg ccattgtctt tatacaatgt ttcatagcca tttttaagcc ctagtgtaag
    15841 cggagttcct ccgattggtg tacgatttaa tgctgtattc tcacttcccg tcaaacgatt
    15901 gatatcattt gtcaatggaa agatgtccga actgatttgc tcatggttat tgatagctgt
    15961 actattatgg taaaaattca ccataccgat tcgaatatcc agactcgggt cagagagcag
    16021 atctttactc atttcgttta ctgcctcaat caacgcagca tttttagttt gactattggt
    16081 cccagcggtt aattcactca tactcgctga cttatctaga acaaaaacga tatcgattgg
    16141 actgatttcc tttttctcgc ccccgatgac atctagatag acattgtaaa gggttgttgc
    16201 atcttcagga tgtagatcag atgctgtgct gcttggcaga acaggttcta cccatttttt
    16261 gatgtacgcc ccgttataat ccaagtaact gttttcaaaa ttccgttcat ttccccaaag
    16321 ctgaacggtt tgagctggaa cgtttctatc ttcttcgcct gcagttgatc cattattagt
    16381 agcatagtcg taatttcgcc agttcgctcc cccgtttgta taatcactat catcgatcaa
    16441 gttctctccg tcatatggcg agcctttgtc tccttgatag cgttcattgc tttgtgtcca
    16501 atttcctaac acttttgtat agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt
    16561 ttcaacttct ccgctaggat agatatattc ataggggtct tgcgtagctg ttggcccaag
    16621 acgctccaaa ataccgttga ataactggcg ttgaatgcgc tggagtgctt gaccgctggg
    16681 tacgattggt tcgtccagct tttccatctc attttcttcg ctacgttgaa tttcagcgga
    16741 atcttctgta tcagtttcat tttcttgtaa ttcttggtta tctattcttt catcaaccac
    16801 cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa
    16861 tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat
    16921 cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac
    16981 gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc
    17041 attggcactt tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat
    17101 atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc
    17161 tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc
    17221 aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt
    17281 atactccttt caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta
    17341 attataattt aatttaattt taattttaaa tcaaatgata tccttttta
//
LOCUS       DAZKOM010000056        17256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000056 DAZKOM010000000
VERSION     DAZKOM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.056"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1921
                     /locus_tag="ACG8NF_001775"
     CDS             155..1921
                     /locus_tag="ACG8NF_001775"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0472255.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2023..3282
                     /locus_tag="ACG8NF_001776"
     CDS             2023..3282
                     /locus_tag="ACG8NF_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0472256.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3429..4730
                     /locus_tag="ACG8NF_001777"
     CDS             3429..4730
                     /locus_tag="ACG8NF_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472257.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4733..5584
                     /locus_tag="ACG8NF_001778"
     CDS             4733..5584
                     /locus_tag="ACG8NF_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0472258.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5597..6412
                     /locus_tag="ACG8NF_001779"
     CDS             5597..6412
                     /locus_tag="ACG8NF_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0472259.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6563..7729
                     /locus_tag="ACG8NF_001780"
     CDS             6563..7729
                     /locus_tag="ACG8NF_001780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0472260.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7959..8606
                     /locus_tag="ACG8NF_001781"
     CDS             7959..8606
                     /locus_tag="ACG8NF_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0472261.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8690..9634
                     /locus_tag="ACG8NF_001782"
     CDS             8690..9634
                     /locus_tag="ACG8NF_001782"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0472262.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9769..11220
                     /locus_tag="ACG8NF_001783"
     CDS             9769..11220
                     /locus_tag="ACG8NF_001783"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0472263.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11241..12077
                     /locus_tag="ACG8NF_001784"
     CDS             11241..12077
                     /locus_tag="ACG8NF_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0472264.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12077..12736
                     /locus_tag="ACG8NF_001785"
     CDS             12077..12736
                     /locus_tag="ACG8NF_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0472265.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12862..13551
                     /locus_tag="ACG8NF_001786"
     CDS             12862..13551
                     /locus_tag="ACG8NF_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472266.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13557..14513
                     /locus_tag="ACG8NF_001787"
     CDS             13557..14513
                     /locus_tag="ACG8NF_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0472267.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14557..15069
                     /locus_tag="ACG8NF_001788"
     CDS             14557..15069
                     /locus_tag="ACG8NF_001788"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472268.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15325..15873)
                     /locus_tag="ACG8NF_001789"
     CDS             complement(15325..15873)
                     /locus_tag="ACG8NF_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0472269.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15962..17143)
                     /locus_tag="ACG8NF_001790"
     CDS             complement(15962..17143)
                     /locus_tag="ACG8NF_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0472270.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5658 a         3078 c         3458 g         5062 t
ORIGIN      
        1 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
       61 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gtttctctat cacttatagt
      121 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
      181 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
      241 taggaaaaat gatatcaaaa aagtatatgt gctttgtgga gatccatata ccataacaac
      301 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
      361 ttattgggaa attgaagtga cgagcgaaac aaggcgctta cagtatgcgt tccatgttgt
      421 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
      481 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
      541 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
      601 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
      661 ccgagaagac ttcttcgggg gagatttgca aggtgtgagg gatcatttgg attacttgac
      721 ggaccttggc gtcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
      781 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
      841 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
      901 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
      961 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     1021 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     1081 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     1141 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     1201 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     1261 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     1321 tgcattcaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     1381 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     1441 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     1501 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     1561 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     1621 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     1681 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag tatatgatcg
     1741 tgaagataag ctctatttca cacgggaact agagggacag atcatctatg tttactttaa
     1801 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     1861 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     1921 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     1981 taagggtgta acagcacgtt gaatacacag gaggaaatga agatgaaaaa aagaagtgtg
     2041 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     2101 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     2161 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     2221 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     2281 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     2341 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     2401 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     2461 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     2521 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     2581 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     2641 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     2701 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     2761 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     2821 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     2881 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     2941 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     3001 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     3061 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     3121 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     3181 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     3241 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     3301 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     3361 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     3421 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     3481 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     3541 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     3601 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     3661 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     3721 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     3781 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     3841 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     3901 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     3961 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     4021 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     4081 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     4141 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     4201 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     4261 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     4321 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     4381 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     4441 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     4501 atttcaataa tttctccatg atctatctgt ttaacaatgg tggtcccgga agcgtaggcg
     4561 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     4621 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     4681 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     4741 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     4801 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     4861 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     4921 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     4981 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     5041 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     5101 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     5161 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     5221 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     5281 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     5341 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     5401 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     5461 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     5521 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     5581 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
     5641 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
     5701 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
     5761 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
     5821 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
     5881 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
     5941 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
     6001 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
     6061 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
     6121 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
     6181 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
     6241 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
     6301 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
     6361 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
     6421 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
     6481 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
     6541 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
     6601 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
     6661 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
     6721 tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
     6781 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
     6841 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
     6901 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
     6961 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
     7021 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
     7081 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
     7141 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
     7201 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
     7261 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
     7321 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
     7381 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
     7441 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
     7501 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
     7561 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
     7621 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
     7681 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
     7741 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
     7801 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata
     7861 ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg
     7921 taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata
     7981 ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat
     8041 ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa
     8101 aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag
     8161 atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg
     8221 caattactag tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg
     8281 tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag
     8341 agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc
     8401 tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag
     8461 gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag
     8521 tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag
     8581 tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt
     8641 ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga
     8701 aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg
     8761 catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa
     8821 gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt
     8881 agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc
     8941 tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg
     9001 gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg
     9061 gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga
     9121 ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg
     9181 aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa
     9241 tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga
     9301 tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga
     9361 gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga
     9421 aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct
     9481 atgtggcgca agcatgttac agattggtac agagcttcat aaagaagggc cggaaatttt
     9541 ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga
     9601 cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc
     9661 ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta
     9721 tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca
     9781 acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat
     9841 atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca
     9901 ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt
     9961 atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga
    10021 tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa
    10081 gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt
    10141 acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc
    10201 ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct
    10261 agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa
    10321 ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct
    10381 attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga
    10441 agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga
    10501 aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa
    10561 gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg
    10621 acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat
    10681 ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga
    10741 aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc
    10801 gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta
    10861 catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc
    10921 gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa
    10981 gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat
    11041 acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa
    11101 gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt
    11161 caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag
    11221 cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct
    11281 tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta
    11341 atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt
    11401 attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta
    11461 aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg
    11521 tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa
    11581 aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa
    11641 aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac
    11701 ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca
    11761 atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa
    11821 atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac
    11881 tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg
    11941 ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga
    12001 aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg
    12061 agtcggtgag tgagtgatgt actttcccct aattgatttt tccctcatgt ggacttcact
    12121 tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt
    12181 aggaaatttg gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa
    12241 tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt
    12301 tctgttatat ttcgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt
    12361 tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc
    12421 ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata
    12481 tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga
    12541 tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc
    12601 aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat
    12661 ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat
    12721 ccatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt
    12781 tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa
    12841 atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta
    12901 taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac
    12961 caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga
    13021 tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt
    13081 ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc
    13141 cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc
    13201 tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa
    13261 gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg
    13321 gagcgaggcg taaaatattt tccaaaagat caattgtaca aagaattgaa aagacgttca
    13381 gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct
    13441 agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat
    13501 ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt
    13561 actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc
    13621 gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc
    13681 ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc
    13741 cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa
    13801 atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt
    13861 cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga
    13921 aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct
    13981 ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa
    14041 gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg
    14101 gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc
    14161 ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga
    14221 aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag
    14281 caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag
    14341 gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga
    14401 aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat catccaaaag
    14461 attggcttaa atattattca aaagttcagg aactatatga agtgatccaa tgattagata
    14521 atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa
    14581 gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa
    14641 ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt
    14701 gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta
    14761 gtctcaggcg tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt
    14821 ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca
    14881 aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt
    14941 atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact
    15001 tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc
    15061 atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac
    15121 aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa
    15181 tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct
    15241 ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgatc tctcggctta
    15301 tcctacgagt attattcgat acatttatat caataatcct ttttttattt tatctgcagc
    15361 ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt
    15421 caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc
    15481 tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg
    15541 gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta
    15601 acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg
    15661 agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc
    15721 agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta
    15781 agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc
    15841 agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca
    15901 agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat
    15961 tttataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca
    16021 aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg
    16081 caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa
    16141 ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa
    16201 attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg
    16261 ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga
    16321 tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa
    16381 gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt
    16441 ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata
    16501 catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag
    16561 tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg
    16621 tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct
    16681 tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag
    16741 attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata
    16801 atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca
    16861 tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt
    16921 caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa
    16981 ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca
    17041 tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc
    17101 ctaatgtgac aaataaacca ataataccta ataaataact caaataatca ctcctattcc
    17161 aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca
    17221 ttactttttt agagtgaaac gcattcattt tccatt
//
LOCUS       DAZKOM010000057        16504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000057 DAZKOM010000000
VERSION     DAZKOM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.057"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8NF_001791"
     CDS             <1..1087
                     /locus_tag="ACG8NF_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0472271.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACG8NF_001792"
     CDS             complement(1305..2447)
                     /locus_tag="ACG8NF_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0472272.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACG8NF_001793"
     CDS             complement(2452..2637)
                     /locus_tag="ACG8NF_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0472273.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACG8NF_001794"
     CDS             complement(2991..3236)
                     /locus_tag="ACG8NF_001794"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472274.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACG8NF_001795"
     CDS             complement(3230..3631)
                     /locus_tag="ACG8NF_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0472275.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACG8NF_001796"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACG8NF_001796"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0472276.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="ACG8NF_001797"
     CDS             complement(5649..7415)
                     /locus_tag="ACG8NF_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0472277.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="ACG8NF_001798"
     CDS             complement(7428..8222)
                     /locus_tag="ACG8NF_001798"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0472278.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="ACG8NF_001799"
     CDS             complement(8232..9608)
                     /locus_tag="ACG8NF_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0472279.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="ACG8NF_001800"
     CDS             complement(9728..10375)
                     /locus_tag="ACG8NF_001800"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0472280.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACG8NF_001801"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACG8NF_001801"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0472281.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACG8NF_001802"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACG8NF_001802"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0472282.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="ACG8NF_001803"
     CDS             complement(13042..14673)
                     /locus_tag="ACG8NF_001803"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0472283.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="ACG8NF_001804"
     CDS             14977..15648
                     /locus_tag="ACG8NF_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0472284.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15808..15999)
                     /locus_tag="ACG8NF_001805"
                     /pseudo
     CDS             complement(15808..15999)
                     /locus_tag="ACG8NF_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5354 a         3192 c         2212 g         5746 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcc
//
LOCUS       DAZKOM010000058        16407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000058 DAZKOM010000000
VERSION     DAZKOM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.058"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8NF_001806"
     CDS             6..245
                     /locus_tag="ACG8NF_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472285.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8NF_001807"
     CDS             312..521
                     /locus_tag="ACG8NF_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472286.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8NF_001808"
     CDS             581..1252
                     /locus_tag="ACG8NF_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0472287.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVLAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8NF_001809"
     CDS             1305..3281
                     /locus_tag="ACG8NF_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0472288.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3296..4048
                     /locus_tag="ACG8NF_001810"
     CDS             3296..4048
                     /locus_tag="ACG8NF_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0472289.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4064..4825
                     /locus_tag="ACG8NF_001811"
     CDS             4064..4825
                     /locus_tag="ACG8NF_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0472290.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            4838..5098
                     /locus_tag="ACG8NF_001812"
     CDS             4838..5098
                     /locus_tag="ACG8NF_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472291.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5095..7185
                     /locus_tag="ACG8NF_001813"
     CDS             5095..7185
                     /locus_tag="ACG8NF_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0472292.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7212..7676
                     /locus_tag="ACG8NF_001814"
     CDS             7212..7676
                     /locus_tag="ACG8NF_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472293.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQINQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7673..7915
                     /locus_tag="ACG8NF_001815"
     CDS             7673..7915
                     /locus_tag="ACG8NF_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0472294.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7933..8235
                     /locus_tag="ACG8NF_001816"
     CDS             7933..8235
                     /locus_tag="ACG8NF_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472295.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8305..10368
                     /locus_tag="ACG8NF_001817"
     CDS             8305..10368
                     /locus_tag="ACG8NF_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472296.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10368..12977
                     /locus_tag="ACG8NF_001818"
     CDS             10368..12977
                     /locus_tag="ACG8NF_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0472297.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            12974..15499
                     /locus_tag="ACG8NF_001819"
     CDS             12974..15499
                     /locus_tag="ACG8NF_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0472298.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPKTD
                     PEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKRIS
                     DLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDNDQS
                     FVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQNVH
                     TGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15551..15883
                     /locus_tag="ACG8NF_001820"
     CDS             15551..15883
                     /locus_tag="ACG8NF_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472299.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            15884..>16407
                     /locus_tag="ACG8NF_001821"
     CDS             15884..>16407
                     /locus_tag="ACG8NF_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472300.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         6086 a         2575 c         3324 g         4422 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtacttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcagaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgagtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ctatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttattggaa tgttaggaat cattgcaggc tgctttcagg
     1381 gatatcgagc atatgcagaa gatatcacgc aaaaaacgcc accagaaaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgactgaag
     1621 attctgatct aaccaacgcg aaagtggacg aaatcgttaa agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacaa acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagattac ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gactgataat acaggttcag gttttttaaa agacattcac ttatatccta
     1921 aaaatattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc
     1981 aaagcaaaca aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attaggagat cttgtatcgg atgatcaagg aaagctgact gctactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcaga agttgtagtg ggatcagata
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacaattca tatcccaaca gacattgcag
     2401 gcggagcaga cggtatcaca gtaaacggag tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg ggatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca
     2521 aagtaacaaa taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa
     2581 attcagaaaa tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg
     2641 tagcaagttt acacggacaa gatcttcaaa tgacttataa catgtatgta aatgacagcg
     2701 cagctgttgc caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct
     2821 taaaagtcga tagcggacta tttggaacag gaatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgctgc taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag
     2941 acggtgtcaa agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag
     3001 ataaagaagg tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa
     3061 caaaagcgcc tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa
     3181 tgaccggtag caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag
     3241 gggcactttc tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat
     3301 tatcaggaag cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact
     3361 ttcagctgta ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca
     3421 aaatcagcag attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt
     3481 ggatgttgct aagattgagt taggcgatcc tgttggtatt cttacaattc taagtatttc
     3541 tttaaaacta ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat
     3601 tacggaagga actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca
     3661 tagtggactt tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa
     3721 gttttatatc aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt
     3781 gcaaaaagac gaactacaaa gaaactttgt cacttactta gaaccaaatc ccaatgaaga
     3841 tcgagtaacg ctaatgacat gtacgcctaa ggggatcaac acacaccgtt tcctagtgta
     3901 tgggaagcga gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa
     3961 attatcttgg aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc
     4021 gctatttgtt tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg
     4081 gctatttgtt tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc
     4141 agacagacaa gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac
     4201 gattcaaagc agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa
     4261 aattgctgaa gacgatcaaa tagtcaaaca gaccattcac gttcaaaaaa ttatttcttc
     4321 cgaacaatta gacaaccagg ggcaaaagct tgaaaaacaa gaaggagtaa atggcgcgaa
     4381 gttttctgtt tatgatgtat cggacatttt acaaaagatg gatgttaagg atctcactac
     4441 tgaccaaatt gaatcccaat taaaagatcg agtgaaaaaa ctatccagtg atcaactgaa
     4501 gctaatttct aatggagaaa caaagacaat tgatcaacaa acaggtgtct ttgaattctc
     4561 ggtagaagta caggcaaatc agaaacaagc ttattatatt gtcaatgaat caagtcctga
     4621 aaacatttct aatagcgagg atattttgct attaacacct gtttctgata aaaatggtga
     4681 attcttaaag gatgtgtgga tttatcctaa atcagaagct tcgcaaccta aagaagaagt
     4741 aaaaaaaatc gtatctactg gcgtaaagaa aaacttcttt gagaattgtt gggattttgt
     4801 tactcacttg ttttctagag attaaggaag aaataaaatg aaactgatca aattattatt
     4861 attcaatttt tcttgtttaa ccgcagtctt tttgtactgc ggtttattat tttctaaact
     4921 atctattttg ccaagctacc aactatacct aatgaggatt ttaaacatac cagctgcttc
     4981 catgccgcta gatattatgg aaaagctcat taggaatcca ttatttacta cgatcaattt
     5041 gatcgttatt gtcggaattt cactagtcat ttataaaaaa gaggggatga agggatgaat
     5101 tttaaacact cgtggacaag acggttaaaa tatgtttttc ccataatgat gatgttaggt
     5161 acattgtttg ggttaaaaac agccaatgtc tttgctgaaa aagttattgt cgatccgtca
     5221 aatccaatcc agaacgtaaa gaataatttt attattccaa aagatgttcc taatgcaaaa
     5281 acaaatatta ctgtaaacgg agcgtcaaaa gtttacaaat atcatgttga tgccaacaaa
     5341 ggtggtttta cagacgacta cgtaggatta gtggagggaa acaaaaatat acgctatcct
     5401 tctttccaaa aagaatacgg tgaaacaaac cttgttttag aaggcgtaac aaccaacacc
     5461 aaagtcaata ttgactatgg aaaaatcggt acgtataacg ggaaaaaagt caatatcaag
     5521 ctagttctat ctaatattca cttatactcg gatacgttgc cgtggaatct actagataac
     5581 aactatacaa aaactcattt tcgagacgat ggctataaaa atacgaatgg tgcaatgagt
     5641 aaatctaaaa aaagaacagt actttggatc tcagacaatc ttttctcagg aattgtttac
     5701 cattcaacac aaatgaatgt gcagctagta gccacatatg aggacggtag ccccgttcaa
     5761 ttttcaggag atacatttat cagttttaac tctttgaacc ctgcaggagg aaaaagcacg
     5821 gatcttaaag gagaatatgc ccactatgac aaaatgaata cgacagattg gtatgtcaga
     5881 aaagatacgg ttctttcaga atttaaatct ttctacaaca atctgaacgt ggtaggtggc
     5941 cacccaggag gaagctctaa gctgacacaa gcggataatg atttcaataa tttgcatgat
     6001 aaattaggag atccgaagtt tggtcaaggt actgtgtcct ttaaaatatc tgaagcaaat
     6061 ccgacgtttg tgattggttc ttctaatgtt caaacctggt ttactttatc ttctgcgaca
     6121 atctttagtg ttgttcctga tcaacctgaa aaaacagggg ttgataaaaa tggaaataac
     6181 gtgaacgata aaatgttgca agtaggagac acgattcaat atcgaatcaa acagaaggtg
     6241 aatcgattag gagtggattt actagcggtt tacgatcgat ttgagttaat cgataattta
     6301 ccaaaagaag taaattacgt cgatgcacac gttgaaagtg gtacaaataa aaaattcgat
     6361 gtatctggtg aagtgaccta tgacaaaaca aaacaccagg taaaatatgc agcgaaagct
     6421 gatacattaa aaaatcgaat gaagtataac ggcgaaactt acgaattagt gatcaatgta
     6481 aaagtcaatg aattggccaa tcaaaatagt gtagccaaaa atcagggaac gtcgatcatt
     6541 aataaagtag aaaaggatac aaatatcatt accgtttatt tccctaaaat tccagtgaaa
     6601 gaagtacaac aaaatggaaa agatgtaaac ggacgaaatg acggtaagaa gggtacgccg
     6661 acagctcctt taaacgcagg atctgaagta caatatctag tcacacaaaa atggcacaca
     6721 aaaggcgtag acgctgcgtc agatcactac aaacaattta gtattcaaga tccaattgaa
     6781 gctcgtttga catataaaga aggttctgcg caagtaatag ataagagtaa aggaaaagat
     6841 attacttctg aaggcacact aacctatgat agcaattcaa gaacgctgaa gtgggaagct
     6901 tccgctgact tcttaagtaa caatctttta gatggacgag aaattcagct gatatttaca
     6961 gctaaaactc cattacaatc agaaaaaaat attgataacc aagccgtagt agcagtagat
     7021 aatgtttcta ataaaacaaa tgttgtgaca attggggttg atcctaactt gccacaggtc
     7081 attgttccta aaacaggttc tacacattta gtaacgattt cagcggtcag cttactatta
     7141 cttgtgttag caacttttag ttatgcggtt ctgagataca tttaaaataa aaaaacgaaa
     7201 gaatgaataa aatgaaaaag ttaataatag tagttacttc cttgattgcc atgattgtta
     7261 ttttagtcgg atgtagtaat gaaaaaaata aacaaattaa tcaagataat ggcgtattga
     7321 aatcaggaac tatgtggaaa gaagaagtag gtggtcttgt ttataatttg aaaattatag
     7381 acgagactac ctgggaatac tctgagagtg tatggcatcc agatcctgta caaataacag
     7441 tcaagaggca aaaagactat aaaggtttag aaagatacaa aatagtagat tcagctggtg
     7501 ttagggaatt tataaacaaa tctgattctt tatttattgt tgttccctat gagaagaatg
     7561 gcgtaaaaaa aataattttt cttggatcta gcaaggatga aaagcgaacg aaagagaaat
     7621 tgattcacga tggatcacaa tccaataaat ataagttaca aaagactagc gagtgattgc
     7681 tagtcttttt atttgtcctg aaggaggaat cacagtggca aaagctaaaa cgccatttga
     7741 aaaagccgcg tctaaaatct tagaacgccg cggtgtatct gaaacacagt ggaaacaaga
     7801 agtccttgaa aaagcacaat ttgacctatt acggggaaaa gacaaggact tagaaggata
     7861 tatctatcaa cgtgaacgtg aaaagcttgt tttcgatgaa attattaaac actaaaagga
     7921 gcgaacaatt ttatgtttga atcaattaca atgaattttt taacacaacc gaaagtagta
     7981 gctgcggttg atatttttag agcattacaa actgccgcaa ctagtttaaa aaacaatctg
     8041 atgatttttg ctgctgctgc ggccgttgct tccattgtta ttgggggtct tttcttccta
     8101 tttggacgaa gaggagctga aaatgggaag atgcacgtag gctatgtatt gatgggggta
     8161 ggagttattt tagcagcagc ttctattgtg gtttcgttat ttggattgtt tggacaaaca
     8221 cctcaaggat tataagaaag taaaaaagta ggagtaagaa gtagtcgata ctttttactc
     8281 cttttttatg aaaggagaat actcatgtac tttacgattg caggcaatac caaaacagat
     8341 aaagagttaa taaaaaaagc aacgaaggaa gtaggaaagc gtacttcttt tttaccgaaa
     8401 acaattaaat attctgtcat tgaaacggaa gaacagctac atgtgttagg gaaagtactg
     8461 gaacacgaaa gtcgatcttc tgttaagttg actctcacgt tacatgacac agaaggaaaa
     8521 atagagaaag ctggtacaga atacgttttt ggcccactgg attatcaaat ttcagaaggt
     8581 ttctatccta cattgatgga tttaatccgc cttgatgtgt ttaaagaaac ggatgaaact
     8641 atgagtgtgg aagagtctta tcgaaacgaa aaacttttac aagttttaga agaagagtta
     8701 gagagcgacc aaagaaaagt gaatcaaggg agtgaagtcg aagtaaatga agaagtacca
     8761 tgggaacgac caatatttcc aaagaaggag tcaaaagaaa aagagacagt ctatgttgac
     8821 gaacaagctg atattccgac tcagaaatcg atatttgagg aagagaacga tgtgatgggt
     8881 caattggagg tagatccctt tgaagaaaca atggaggatg aagagacggc cgaagtagaa
     8941 gttgatccag ttgaagcgaa tgaagagtca acatggactg aaggggattc tcaggaatat
     9001 attgaaccac ttatcagtga agaagtagaa aaaaacgaaa tagagataca aaaggcaact
     9061 gtatttgatt atattttgga tcaatatccg ccagagcatg aatggatgaa agaaaaaaac
     9121 aaattatttt taaatgagct ttattcgaaa tatcaattac cccaaaccta tcaagcattt
     9181 ttagagagta aagaagaatt aatctcacag ggtaaaggca cacttttaga acagttagag
     9241 tctgtccaat caacaaattg gcatgagctt gcacaagaag atttattagc cacctttgaa
     9301 gaacgtcaac aagaaacaga tcaagaaatt aatgagtatg cgacggaaca gtcgtctaat
     9361 tgggagaaag caaaagagga actaaataaa gaagaacagc gagtgattga agaagaagta
     9421 caaaaaatta aagagcgata cgaaaagaaa cgcgacaatg cgtattcctt gtgtgtagca
     9481 aagatcaata attttactca gacgaatcaa gaagcgttat cgaaagaaaa agaaaacttg
     9541 ttaaaagaac gtattaaaga acgaaaagaa gaattttacc gaggtttgcg tacagataag
     9601 ctaaagatta gcaaagaatt aaatgaacaa ctgaataagc tatatcaaac aacgactgaa
     9661 gttcttatgg aaaaatacga aaagatccag caagaattac aagtgcaaat tccaaaatgg
     9721 aaaaaagagt atgaaattca acaacaagaa ttgaaagaac aagaagaaaa acaacggcaa
     9781 aaagaaaaag agcgcgaaca attagaacta aaaaaacgtg aacaggatct tgcggaacaa
     9841 caaatggcaa tagaaaaaca acggttaaac caagaaaagg aaaaagaagc agctgctcgt
     9901 aaggaacgtc aagaacaaat ggatacattg cgacaagctc agttaaatta tctagcacaa
     9961 cctcaaccaa cgccttcagt agctgctgtt caaaccccaa tgaacaacca aacaaatcta
    10021 actccttcac cacagcgttc ctttaacgga tggatggtgg gatgtattgc aagtttaagc
    10081 ttgcttctag gtggaggaac agtatttgct tttaatcata tcaatcaatc ttcacaggct
    10141 gattctgtgg ccacaacgac gtcaatcaag gaagaagccc aagaacaagc aagaaaagag
    10201 tatgaacaac aattagaaga attattgaat cgtaccaatc agtcaacaac agaatcaagt
    10261 atggaaaata gcaaagagag tgatcctagt aaggctaaag aaggatcaag cgattcaact
    10321 gtacaatcaa acacttccga aagttcagaa tcaaatgagg tgaaataatg cgtgttagag
    10381 aaacaacgag acgaaaagac aaacgattca atttagaaaa gaataggtat ctttctcatt
    10441 tctatcgcta tactccttca ggagaagagc taccagaaaa acatgacaaa cgtatttctc
    10501 aaaagatttt ttatagtggc ttagcttatc ttcctttaaa ctatgttgtt acaagaatac
    10561 cagaagcatt tcatcgattt gagattattc caggaagttt acaaggagtg aatatttttc
    10621 agttacaaga aatgccgcag ttattccatt ttgattcaac tggtttttgg aatcctttga
    10681 ccccaacaac aagtttaggc acacgtttag ttgtttctac gatttgtgtt gttccagctg
    10741 tacttatggg attgaaaatg gacatgtggt ttcgtcctct agcagatggg caaaaaggag
    10801 ataaccgatt aaaaacgtta aaggaaatcc aacaaattta tccgcaaata ccagaatcaa
    10861 agggaggttt tcctggttat ggcggaatac caattactca ttataaaaag tactggtata
    10921 ttcagacgga tacagtaaat acttgtattg ttggtacttc ccgttcaggg aaaggacaga
    10981 tagaagtttt agcaaccatt gacaatttgt ctcgtgcaga aagacaaagt tcaatggttg
    11041 ttaatgatcc aaaaacagaa ttatatgttg ctagtaaaga tgaactagaa gctagagggt
    11101 atgatgtgta tgcatttaat atcaccgatc cactgcaatc aatgagtgat aatccattgg
    11161 cactcatcgt gaaatactgg aagcgaggag acatagacac agcgacacag ttaaccaata
    11221 ctttcacgaa tacgatttat tatgatgcca acgcttcaga caataaatac tttaacgaaa
    11281 atgcgcaaaa agctgtcaat gcgcttattt ttgcgttatt ggaacaagcg gatcagacag
    11341 gggattactc caaattaaca ccgaataatc tcgtcgaact tctttctgag attggtggtt
    11401 ttaattacca agatcctgat aatgaattca agcagctcaa tgctttagat gaatttatga
    11461 atcagttacc acctggaaac atagcaaaaa aacaatatgg ggcaacaaaa attggttctg
    11521 ataaagcgaa aggcaacatt ctatcgacag cgatcacagg actaaatcca tttacactga
    11581 cgaaaattgc gaaaatgact tctcaatcaa ccattgatta taagtccatt ggttttccga
    11641 agtatttgga tctcaaacta cacgagagtc tgttaaacca gcgaattgtt gtagagtttt
    11701 atcatcaagg aaaaaaattt catgaggaac gtgtcaaagt aggctataga ggtttttgtg
    11761 aaattaactt tgattgtcat ttagaacagg gagatcatgt ttgcttaaaa tacgatcatc
    11821 gaggaaaaga atatgttgct tggtatgagt tttctcaaag agtattgaaa gacgaagagg
    11881 gaaatattgt ttatcgaaaa aaacgaggag aaacccattt gccagaatta gaaaaagaag
    11941 ccgttctaac attagatgag gataaaagta acttattagt agagtctatt cggatgcact
    12001 ataccgataa accaatggct atttttatgc tcacacctga ttatgatcca tctaaccata
    12061 tcattgttag tatttttctt agtcagttgt ataaagaatt atctacgcaa tgtgttaaga
    12121 ctaaaggaga taaatgtcac cgtaggattc attttatttt agatgaattt gggaatatgc
    12181 caccaatgga taatatggaa ggaatcatga cagtcacagc tggtagaaac atgttgtggg
    12241 atttgtttat ccaatcctat cagcaacttt atgcgaagta tggggaacaa aacgcagcga
    12301 ttatcaaaga aaattgtcag acacatattt atattatgtc gacagacgat aataccatag
    12361 aagaattttc caaaaaagtg gggaataaga cggttgaaca agagaacagt accatcaatt
    12421 cattaggtat gaatactcat atcaatcgca atgttgattc agatcgcatc ttaactcctg
    12481 aaagaatttc tactttttta gaaggtgaaa cgattgtact cgctccttta aaacgacaag
    12541 atttgaaagg tcataaaata cggccgtatc ctatttttaa tcatggggaa acgaatcttc
    12601 cttatgctta tcaatatatt gaagatttca atccatcgtg ggatatcaat gatatagaca
    12661 tacattgtac acatgcggac ttggatctta aggctttaca aattgattac gattattttc
    12721 tacctgatat ctatgcacgt cgactttatc acaatatgca cgatcaaata gaagaggaag
    12781 ctaaagaaaa catttttatt aaaatcgttc aatctttaga caactcaacg gcacaaaagt
    12841 tacttattca attttatgaa gagaagcaaa tagatcgttt gcaagaatac cttgaaaaat
    12901 tgcgtcaaaa tgaagaaatt acagaggtta taaggaaacg acttttgcta gaagctgaaa
    12961 aggcggtgaa agaatgatca ttggaggaat attttccggc attgccgact ggtggtcagg
    13021 cttatttggg gatggggatg cagatcccaa aaccgtaacg aaagtcttac aagattatgg
    13081 taattatctg cagtatcaag atgtattcag ttggatttgt aatacttttt tatggggttt
    13141 gattaaaggg ttatataatc tcaatacttt actagaaaaa acaatttatc aaagctttga
    13201 tttaaaagac ttattgaacg ccgctggtgt taatgaactg tttcaaagtt tgattagtaa
    13261 ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttaaaat tttttatgag
    13321 taaacatcca ccaaaaatca aaaatatctt ggttaatcta tttatggcca tgattttgat
    13381 ttttggtggt agttctttaa tcgatgaagg gctaaatatt agtaaaaact tttacggaga
    13441 cgtgactgca gcaaataaag cagataaaac tggttcacct gcttttcaaa ttattcaaaa
    13501 taatgtgatt gatgtgagta cgttactaga aaatgatccg aacaaagtag aacacatacc
    13561 aacagacaaa agaaatgctc tcacggcgaa aaattttaaa acagcgaata tcaatgggat
    13621 tattacacct gatcaagcta agaaaatgtc tgaatcaagt gatattagta cagtacaaaa
    13681 agatcggtta aaggcattac agtatcgact agagttagac gataatggga aagaaatccc
    13741 tgttcaaatt agtgatgaag gtttagcaaa atatgtgtat acttcaggct atcgccgata
    13801 cattagtagt ccaggtatta ttttagctgg tgaatcttct ttgtcggtag cctatttatt
    13861 tatcttgttt acgattgtta cttgcattat tgagctggta tttaaaaaat tctatttagt
    13921 gattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcta tagaagatat
    13981 ttctcaatcc ttcttgttat tggcatttac gattttagaa ttacgaatct atacattaat
    14041 gctttcaggt atcggtgatt tgcatgcggc aggaaagatc aatggattct tgtatgtcgt
    14101 tgctttgatt gtactaacca ttgcgctatt caaaggctct caatcagtta cgaaaatttt
    14161 cggcgttgat acaagcctga aaaacggcag taattctctt cttagcatgt ttgccttagg
    14221 aaatattgcg aaaaatgtgg gtagcggagc taaaaacctt gcaagtgctg gtaagagtgg
    14281 tttaagccat ttaaatgata ttcgtaaaaa tggatttggc catcgaaaag atgttacccc
    14341 tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata gtaagaaaac
    14401 agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag aaggtcttgg
    14461 ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa aagccaaaga
    14521 caccttagaa gataaattag acggcacgag agctaaagaa ttggctgaag ctgtgaagaa
    14581 tccatccgaa gcactagaaa aacaaaaaga aaaagcttca gaagtcaaag aaggactaaa
    14641 agatgcgtat ggtgaaggac aagtcaatgc agcagtgaaa gctaatacaa caaccgaaaa
    14701 agaaaatcaa gaaggtatag tgattccttc agtcaaagat caccaagaag cgttgcactc
    14761 taatgaaaat tcgattgaag agaaaaattc catagaagaa agaaattcag aagaaggaaa
    14821 aaatggacag ttaccaaaaa ctgatccaga acaatatcgc tataaaccta acagtgacct
    14881 tgaaaatgag aagcgaattt cagatttatc aaaagtaaat caagaagaca aagcagaaaa
    14941 tgagggacag ttaccaaaaa ctgatccaga acaatatcgg tatagatctg ttaactccga
    15001 aaatgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa cggatgagga
    15061 ttccatcgtt ctgccaaatg ataatggaat cgaagttgga actgaaaaat caacgaaaat
    15121 tggtttacct aaggcacaaa atttaccaat gaaagatagt cgtttgaatc aattcaagga
    15181 taatgatcaa agttttgttc atcaatcacc tgcaggtact gttcaaatga aaccaagtcg
    15241 tttgcctgaa aatggaaatc tgacaactgt ttcttacaat ttatctgaaa gtggtgagac
    15301 agggaatgcg tccgttcaaa atgttgtgac aagagaagct gcaccaaaac ctattcaaaa
    15361 cgttcataca ggttacaagc cagcaaatgt ggcatttact gcaccaacaa aaataaatgt
    15421 gacaagagaa acaaaaaaca ggaacaatgc attgaaagaa caactattga gggatatcaa
    15481 acaggatatc aaaccataaa aggcctacaa gttaggcctt ttttctatgt atgaaaagga
    15541 gaaatcattt atggataaat atagtaacat gaaaaaccct ccaagattta gtggagaatg
    15601 gaagtttttc gcttttttct ttttagatct attaacgctt ggtggctgct ttttttgggc
    15661 gaaaatggtc aataatcttg tccacttacc actctttttt gcactcttga attatggctt
    15721 atggattgcg atagcgttag tgattgtttg gcgaccctat aataacccag gtcaacgaca
    15781 aatttttgcg attatcgagt ctattttttt tgcggacaag aaccattatc atgcgttaga
    15841 tcctaaccga aattatcatg gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca
    15901 aaattcagtt gaaaggaaat ccattttgca gtttggaaaa ccagaagagc gctctatctt
    15961 ggacttattt ccatataaat cctgtacaga agagggagtc ttaattacta gagaagatca
    16021 ttttcaacgt tattatcgtg tagtttctac agacgtggaa ggattaaatg aacaagagaa
    16081 actagaacga atgaatcagt tgaccaccgt catgcgtacg tatgtgccaa caattaagtt
    16141 agttagtttg accacagaga cggatctaac ggaacaaatc gtgcaaaagc ggcaattatt
    16201 agataaaaat cgtatggaac agtccttagg aagaaatcta caacgattaa aaaaatatga
    16261 aaagaaatta gtcgaagaaa tccaggaatt gaaacgagca gaacaagaac gtcctgactt
    16321 aagcttcttt ttcgtcattg aaggaaagac aatcaaggat ttacaaacca gaaaccgtca
    16381 gttaatgcga tctagtggca ttttagg
//
LOCUS       DAZKOM010000059        16292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000059 DAZKOM010000000
VERSION     DAZKOM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.059"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..896)
                     /locus_tag="ACG8NF_001822"
     CDS             complement(33..896)
                     /locus_tag="ACG8NF_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0472301.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1092..3191)
                     /locus_tag="ACG8NF_001823"
     CDS             complement(1092..3191)
                     /locus_tag="ACG8NF_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0472302.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3411..4520)
                     /gene="rpoD"
                     /locus_tag="ACG8NF_001824"
     CDS             complement(3411..4520)
                     /gene="rpoD"
                     /locus_tag="ACG8NF_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0472303.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4544..6385)
                     /gene="dnaG"
                     /locus_tag="ACG8NF_001825"
     CDS             complement(4544..6385)
                     /gene="dnaG"
                     /locus_tag="ACG8NF_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0472304.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6567..8882)
                     /locus_tag="ACG8NF_001826"
     CDS             complement(6567..8882)
                     /locus_tag="ACG8NF_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0472305.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9067..9927)
                     /locus_tag="ACG8NF_001827"
     CDS             complement(9067..9927)
                     /locus_tag="ACG8NF_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0472306.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9986..10618)
                     /locus_tag="ACG8NF_001828"
                     /pseudo
     CDS             complement(9986..10618)
                     /locus_tag="ACG8NF_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            10785..11546
                     /locus_tag="ACG8NF_001829"
     CDS             10785..11546
                     /locus_tag="ACG8NF_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0472307.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11572..14163
                     /gene="mprF"
                     /locus_tag="ACG8NF_001830"
     CDS             11572..14163
                     /gene="mprF"
                     /locus_tag="ACG8NF_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0472308.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14351..15538)
                     /gene="tuf"
                     /locus_tag="ACG8NF_001831"
     CDS             complement(14351..15538)
                     /gene="tuf"
                     /locus_tag="ACG8NF_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0472309.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15906..16262
                     /locus_tag="ACG8NF_001832"
     CDS             15906..16262
                     /locus_tag="ACG8NF_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0472310.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4456 a         3378 c         2875 g         5583 t
ORIGIN      
        1 taagctagtt aagaaaaaca tgcaagggat tctcaattag attttttgac tagttttgtc
       61 acaccgtttt cttgtgtaat caatccttgt ttcaaaagat tacctaatgc gcgcttaaat
      121 gctcctttgc tgataccaaa agctttcatg atttcttctg gatccgattt atctgtaaag
      181 ttgatttgct gatctgcaga acgttctaaa atggttaaaa tcatttgtgc atcatctgat
      241 atagcttcat atgctcttgg cttcaatgaa agattcagta caccgtctgg tcggacacca
      301 atcactcgac cttttatctg ctcgcctaga cgaggttcat tatatcgttc agaaggatgg
      361 ataaacccaa gatagaaatc ttccgttaaa acgtacgtac ctgttaattt caagcggtat
      421 gctgttccta tcacatcttt gtttttcatt tcttctgttc catatttgct taaagatttg
      481 aatatttttt catctgctaa actgccccaa atacggtctt ttgcatcaac tctaagggaa
      541 atcatcaagc gatccccttt tttaggccat agctctgtca ttgtcggcaa ttcatcaaga
      601 gatacgacaa agtctttatc tggaagaccg atatcgacaa acgcacctaa atcttttcgt
      661 gtagcagtaa cgattccaaa agcgtaatga ccgatacggc ttttaggaat ttgtgtagtg
      721 atccgatttt catgtttttg attttggtaa ccgaatcctt caactgcttc accgattttg
      781 tgttcacctt ctgatttgct taagccaaag gtttgtccgt ttttttggac aaagtagttt
      841 ttttcatttt catcaataat aatacctgta aatatagttg ctaataaatc gttcattttt
      901 ttctccctct atagtgaaag gttttcttaa tacatttctt tgctatcata acataatgtt
      961 atctacttga atagctgagc cttggtttgg aaaggtgaaa aaaggagtat gacaacagtt
     1021 gtgtcatact cccctttttc gaataaacgg tgttaaacgg caggctcaca gcctctttca
     1081 tttgcctaaa attaattgct cccatttcct ccgaaccatc cagttttaca gttgatggtt
     1141 acgatgtaaa atggcaaagc actttctcca atgcctttca tcaatgcaga ttttgttgaa
     1201 gtggcatata gattataata gccttcccca ttttcgattc tggctttttc taatctgtaa
     1261 cctccttcaa cgatatatcc acgttggata catgtagcaa tcactttgct ttggacacct
     1321 ggtgcccacg tccatgctgg attagatgca tcggcaatat cacttgctct ggcagatttg
     1381 attggctgat tcgctgagtt tgaagaggaa ggggtcgagg atgccgcttc ttgctgtgct
     1441 gcttgtgcga ccgcagcttg ttgagcggct tgagctgcag ctgctgcttg agcggcttgt
     1501 tttgcttgtt cttcagctgc ttttttcgct tcttctgctt ttttcttttc ctcttcttct
     1561 tttttctctt tatcagtgag catcttatct acttgtttca attgttcatt caactgagca
     1621 gatagatctg tatttttcac tttttctact gcttcttttg ccgtcttgta ttggtcgcgt
     1681 gttgctgaat ccactacttt tccgtcttta aagactatag ctgttttttc tttagctgtt
     1741 tgcaattcag catattgatt tttagcttct gtttgtgctt gttgaatcaa ttttccaaaa
     1801 tctgtgttat ccgtttcgat tgcttggatc tctttatctg ctttcaactt gatgtcatct
     1861 gctagatgat catcgacgat ggcaggtgct atgaaccact cattcacttt ttggatatct
     1921 cggatcttag attggatatc ttggtaagtt gtttctaact ctgaatatcc tttttcattc
     1981 ttaagactgg ataaatcatt ttttagtttt gataaatctt gatgaatcat atctgcacga
     2041 ataaattgat ggtgatcatc tgtataaaag gcagcaagtt tctcatgaag ttcttccatt
     2101 tgttgctgtc tttttgcttc tgctgctact tcggcttcta ctttttgatg atggtcataa
     2161 taagcccagc ttcctccacc gagtagaagt actgctgcta aaacgagata aggtactttt
     2221 tttgattttt tcttttgcgc tttatctgga tctgtttttt ctaagtttga ttcttgatgt
     2281 gttttttcag tatctggcac gattgttatt gatttcgctt ctgcttcaaa cagcttaggc
     2341 tggttctctt ctttttctat ttttgcttca agatcagtac cctctttgct tacagattga
     2401 ttttcagcat cggattcact atttatttca tcgggctctg ttgacttttt gtctgtgttt
     2461 tcgagtattt cttttttctc tgcttccggc agtttattcg ttttttcctt ttctaattgt
     2521 ttttcagtaa atacttcagt tttttctgtg atatccgttt ttacgtcttt tatttttact
     2581 gtttctttac ctgtggcgtt ttctctatca gagtcttttt cagtttcttg ttccttttct
     2641 ggctctactt tgttttcctg tgcaattttt tcactgatca ttttgagcag ctctttcgcg
     2701 tgctcttctt ttgtatcttc acgatgtgct ttgatataag cggatagtat cgaattttct
     2761 tcctcattat cgacgatttc tttcagttca gctactcttc ttttttgttt tgattcaagc
     2821 gtttcttctt gattctttcc tgattcttcc gaagtctcat gaacagttgc tttcttctgt
     2881 tcatttttct ctatattagt tacttgatcg tgagcaggtg gttcttcgtc ttctggttct
     2941 gctttttcga ataattccat tacagattct agaggtaaat ctttcaattc cgaccattcg
     3001 attggatcgt ttaattctgt atcaggaaag actgcatctt cagttgattt atcctgcata
     3061 gctgcatctg tatttgtttc ttcaattgat tcttttattt gctgatcttc tgcagttatt
     3121 tgatgaccgc agtctggaca gatagtattc tgctccattt ttttcgatcc gcaatttgga
     3181 cattttttca tcacttagcg atcggctcct ttactaggtt tacgctacca tttaatctat
     3241 catattctat acgtcacgcc aatagcttat ttttaggatt tacttaaaaa taatgaatga
     3301 atttccttcc tatagaaaaa aaagcttgag actgcgtagt ctcaagcttt ttttctttta
     3361 aaataaacca aacagtttta ctgatttctc ttctggtcaa tagtccacgc ttattctaag
     3421 aaatctttta attgttttga acgagaagga tggcgcaatt ttcttaatgc tttagcctcg
     3481 atctgacgaa tacgttcccg agtaacacca aatactttcc ctacttcttc caatgttctc
     3541 gtccggccat catctaaacc aaaacgtaag cgaaggacat tctcttcccg atcagtcaat
     3601 gtatctagta catcttccaa ttgttctttt agtaactcat aagctgcgtg ttctgctgga
     3661 cttgttgctt cctggtcttc tatgaaatca cctaagtgag agtcatcttc ttcaccgatt
     3721 ggcgtttcaa gggaaacagg ttcttgcgcg attttcaaga tttctcggac tttttctgtt
     3781 ggaagatcca tttccgcacc gatttcttct ggcgtaggtt ctcttccaag gtcttgcagc
     3841 aattgacgtt ggatacgtat caatttatta attgtttcaa ccatatgaac tggaatacgg
     3901 atcgtacgtg cttggtcagc gattgctcga gtgatggctt gacgaatcca ccatgtagca
     3961 taggttgaaa acttaaatcc tttgcggtag tcaaattttt caactgcctt catcagtccc
     4021 atgtttcctt cttggatcaa gtcaaggaac tgcatgcctc gtcctacata acgtttagcg
     4081 atacttacta ctaaacgaag gtttgcttct gccagacgct gtttcgcttc ttggtcgcct
     4141 tcttcgattt tcaatgctaa cgctacttct tcttccgcag ttaaaaggga gacacggcca
     4201 atttctttca gatacatacg aaccgggtca ttgattttta caccggttgg cgcagaaaga
     4261 tcttctgttt ttgcttgttc agcttttttc tcagcactct tcaagctgtg aattgatgga
     4321 tcaccatttt catccaccac actgatacct gcgtcttcaa tcttttggat caatttgtcc
     4381 atttcatctg cattcaaagt gaatggtgta gcaagctgat tggataaatc atcatataca
     4441 acttgtcgtt tgggtttgtt ttctttgatg aaagctgcta ctgctgcttc atacgttttt
     4501 ttatctgttc catttgccat aaaagaaggc cccccttgag tagctatttg gcttgtttta
     4561 actgtttcgt taaattgatg atttcaatcg tcaattcaag ctcaagctgt ttgtttccag
     4621 ttcgtctggc ttcttgctgc atttgttttt ttaatgttat ttcatcagct aagccagatt
     4681 tgctgatcac atctagcaag tcttggattt ctcgtggcga actttcttct gccaccggta
     4741 aatattcgat ttccaccacc aaccgcctta aattttcatc ttgcaaaaag tctaaaaact
     4801 ctgcaaggtt gaattcataa tgttgtgaga catatgcatc gaataaaaaa taaacttctg
     4861 catatgcatc atgaataaac tgaaattctc gttcttttaa aatattacgg acagaaactt
     4921 ctttaaatgc acgatataat agaatctgct ctgccttttc tacttgcgtc aaaggtctct
     4981 ttttccgtgc agtttcttgt ctcgtaacct gagtgtgttg actttcctga ggttcttgac
     5041 gattttgctg atttgtcctt cgaagatcgc gcagttgttt ttgtatcgtc tctctgccga
     5101 tctgaaactc tgttgacagt tgagatagat acatatcttg ttcgattggt gaaacaactc
     5161 ggctaagttc aactagtaat tcttcgaggt attcgatctt gtctttttca ttatctaaat
     5221 ttttcccttg ttttaaataa cgtgatttaa aagtaaagac tgtttcgcgc ccatgagcca
     5281 ctaattcttg gaaagatgct tctccatact tacggagata ttcatcagga tcaagctttt
     5341 ctggaacgac tacgacagaa agtgctaatc gactgttttg ccgaagcaat tcaatccctc
     5401 gattggttgc ttcaaaacct gcgttgtctc cgtcatagca aaagaccaat tctttcgtca
     5461 gcttttccat ttgttggatc tgttgagctg ttagacttgt ccccatagaa gctaatccag
     5521 ttttgatacc agcctgccat gcagcaatca catccataaa cccttcgaac aacaaaattt
     5581 ctccgttttt ccggatatcg cttcttgctt tatcaagatt aaataaaact tgtcttttat
     5641 tgaacagttc tgtttctgga ctattcaagt atttgggctg atctttttcc tgcccttttt
     5701 caggtgcaag ccagcgtcct gaaaaaccga tcgtctggcc ttgagaatta cgaagtggaa
     5761 acatcactct ttgataaaaa cgatcagaca tacggccatc atcatgcgtg acaaacaatc
     5821 cagagcgttc taacaattca tttgaaattg attcattctt gaatacttgc tgaagaaaag
     5881 aacgctcatt aggtgcaaaa cctatgttga attcttcaat taattctaaa gacaacccac
     5941 gactttgcaa atattctaaa gctggctgtc cagcttttgt atgcaatagc atgtgatgat
     6001 agatttccgc tgctttttca tgagcagcaa tcagtttccc cgtagcagat tcaggatttc
     6061 ggacagctat agtacgctga cgccattgat cttctaacgg tatctgttcg aagtctgcca
     6121 cttttaccac tgcttctgga aaacttagac cttctagttc ttgaagaaaa gtaaacacgt
     6181 ttccgccttt accacaacca aagcaatgaa aaatctgctt gtcttctgca acagaaaaag
     6241 acggtgtacg ttcttcatga aaaggacaca gccccaagta atttttacca gactttttca
     6301 actggacata ttgtccaatc acctcaacga tattcgtttg actgcgtact gtttcgataa
     6361 cctcttgagg aatccgttga gccatttttt caccccctgc tctattgata tcttttgggt
     6421 ttaacagaag atatttcagg agaataaccg gtaaaaaatc gcactaactg aaatcagtgc
     6481 gattttaaag gacacaatta tacatgtttg attgtatcat atttcattgc tttttcaagt
     6541 ataacacatt tctatctcat taagcatcaa tttgctactc ttttcagtaa ctgattttga
     6601 atatcagcct tccagtacaa tactcgaaga acaatattta cgagcatgaa tacaaagaag
     6661 tatcctacgc tctctcccaa ccctaagttt acttccaaaa ttccatggaa cagcattgca
     6721 gctacatata tccctacgat cgtcgttact gctaggaaga tgcgcaatgg gttgaaggga
     6781 taacaagctt ttacaactgc catgcaactg atcccaatca gcaagtaata catcaaagta
     6841 gtagtttcag catttcccca tgcgaatgtt tttccaagta gatacaccgc aatgatattc
     6901 actactacaa gcaaggcatt tggtaaagca ttaataagtg ctgtaggcaa ataacggtaa
     6961 gtgatttttc ttttatcttg ttcaaaagat aaaaagaagg aaggataccc ttcaatcgct
     7021 aaatcaatca gcgtgatttg gatagggata aacggaaatc caattgctgt aacggcacat
     7081 ataatcgata aaataaatga ataaatcgtt ttgataaaaa agatgcctga gactttcgtc
     7141 acattgttga ccactcggcg tccttcaaac aggacttctg gcaatgtagt aaaatcagag
     7201 tccaacaata ccagattcgc aatctgacgt gttgctccat caccttctgc catagcaatg
     7261 ctgcaatctg cttctcttaa cgccaaaaca tcattcacac cgtcgcctgt cattgcaacc
     7321 gttctgccgc tttctttcaa ttcatttacc agcaatttct tttgctgcgg actcactcgt
     7381 ccaaaaaccg tatagcgatg agcagcttct cgcacttcca tttcttcagt aatttgagaa
     7441 agatcgacat acgcatcata attcggtaat cctgcacgtc tagcgatatt ggagacagta
     7501 aaaggattgt ctccagaaat cactttcaga tccacacctt cctcatgaag ataagctagt
     7561 gtttcttgag cgttttttcg gattggatca tcgatttcaa acagagcaac cggctcaaca
     7621 aatgctggtt tttcttcgtt caattgctga tttggagcga tccccaacat aagtacgcga
     7681 tagccattcg cttgggctgt ttttagactg tccggaagcc gatcttcttc caccaaacgt
     7741 tcaggcgctc ctacgtacac gactcccagt tctggaaatt ccatcgcgcc ccatttgcga
     7801 tcagaagaaa atgcgacttt gtttgtcaca tcataccgat tagaaagctc aaaataatct
     7861 ctcatagccc gcatagtgat gttgttatca ctgctttctg ccagatacgt ccccataatt
     7921 tctgggatgg ctttctcgta cagcggatgt agtgcttcta cccgctggat actcattttc
     7981 ccttcagtaa tggtccctgt tttatccaag cataatgtat cgacatgggc cagcgtttca
     8041 attgaataca tgtcttgaac aaggattcgt ttttttgcca gtttggttac ccctgtcgtt
     8101 aatgcaatac ttattagcaa taccaaaccc tttggaagca tgcctaacaa agctgctgca
     8161 gatgcaataa cagaaatttt gatagaagca tcgcggatga acagtgcttc caaaaatagt
     8221 atgatcccca aaggaataat cacgaaacta gtgaatttgg atactttgcg aatcgaatct
     8281 acaagttcag aacgaatagc tttgtgagtc tttgcctcgc tagtcaagcg gacagcataa
     8341 ttatctgcac ctactcggat aacttcagcc atgacagaac cgcttgtcaa aaagcttcca
     8401 gaaagcaatt cagctccagt ttctttgaca atcaaatcag attcgcctgt caaaagagct
     8461 tcatttgctt ctgcccgtcc agatataacc tgtaaatcgg ctggtatctg ttctcctgct
     8521 ccgattttta ccaaatctcc taataccagc tctttagtag aaatcgattg ttctttaccg
     8581 tcccggatca ctagtacttc tgattgggaa acaatcgata atttccgtac aagatttctt
     8641 gcatgtattt cttgataaat tccaataacg atattgacga caataattgc taaatacgcc
     8701 atgttggaat acgcacctac aaataacaga caaatcccaa taccaaagtt cagaaaattg
     8761 aataacgtca atatattatc gctgaatatt tgttttgtac tttttgatgc atcttcctgg
     8821 tagtcatttt gctggccaga agccattttc tgaactacct cttgctgtgt aagtccattc
     8881 attttctttt tctccatttt ccgccaactt tcaaaaataa tcacgtgttt aagtctagcg
     8941 tgttccaaga ttaaaaacaa cttgttttta taaaatttat agaaaaacac acgaatgatt
     9001 ttacaaaaaa gatgccaacc tttttgtttg aatcacccgt gtgctgtttt tattatcaat
     9061 ctatctttac ttatattgtt cctccaaacg gctcatccac caacctagtg ctgtaccggc
     9121 tataaaaaga acaggacaag ctagtacccc agcaactccc aatcccgcgt aatcggtagg
     9181 aatgatcata atcgttgaag caaaaactac acctaaaatg aaatggaata gcttgctata
     9241 tgcctttgag aaaatgtaat ccatgatttt tgacaatcca agaacacaaa gaatcccacc
     9301 aattccgatc ggcacaatca cttcaagtcg tacttcttta aatccgtcag ccatcgcttt
     9361 atacaatccc atatacacca aaaaattaga agggctcaat cctgggacga tcatacccaa
     9421 accaatcaat ccgccggcaa tcagccatgt tccaaaattc tgatcgactt gcccgtcaaa
     9481 taattgagcg ccgtataata aaaacaaata agccagaaca aaagtaacaa ccatgatgat
     9541 cacatcagaa gtatctcttc cttttttccc tgcttctttc cataacgcag gaacagttcc
     9601 agcgatacat ccgacaaaaa accataatat cgttgtttca tacgcaccta ataaaaaact
     9661 gactaaaaaa gaaagtagaa agatgcctgt cactccgcct agaccaacag gcagaaaaaa
     9721 caaaacattt tctttgaaat tttttgtaag gtgggctaaa aaagaaatca gccgctcata
     9781 catgccaaag acagctgcta atgcaccacc gctgactcct ggcaagataa atcctgaacc
     9841 gataaacatt cccttcacaa accgtaacat ccagtctttg gttgtgtacc ccacttcttg
     9901 attttccatt ttttgccttt tttccactat ttgcgtactc cttctttcat ttaattttat
     9961 tcatgattga acatttcctg ctattttact taaagacttt ttcataggtc tctattattt
    10021 tttcattcat tttgacaata tcaggataaa acatgttacc tccattatga tagagatagc
    10081 cgccattgta aagcaaggac tggatccgcc aatatcggta ttgtttccct tcctcatttc
    10141 ctaaaagtgg gctcagcaca tctttagaat acgcttctgc taattctact gtgttttctc
    10201 cgccatgatt ttttacatag gcaatataat ctttcccaaa attatatgct tggacagctg
    10261 tccataaatc gcatccttgt ttctttgctt ctactatcat ttccgccaaa tatttgacac
    10321 cttgatcaat actgtcagct ggattatcaa tgatctcagc ttctccatgc agactttccg
    10381 agctctgcat gagatctgtc ccttttcctt ttgattcggt caatattatc gccttgatca
    10441 aattctcgta gttttccata tgataatttt ttgcagctgt tgttatttct atttgatagc
    10501 tttccactcg ttttaccgaa cgataagttc ttcctcataa aaacaacacc gcaccaataa
    10561 ccgtcagaat gatcatgtaa aaaagaatac ggataataga taatcgtttt cgcttcattt
    10621 catcacctag ttattttgac atcttttttt atcttactat ttctttatct tcttggctat
    10681 ttcttccttg atttctgtgt acgccttaag aaaaaatgaa ttttcctgta ttttttgaca
    10741 tcttacgaaa atgatcgttg tttttcagta aaaacaattg cccaatgact gatataatcg
    10801 ttatacttta cacagtaggt gattatgtga aaaaagaaaa gaaatatgca aaaatgccag
    10861 atgggagcga aatttactat gaaaagagcg gtcaaggctt ccctctcttt ttactgcatg
    10921 gcaatgacgg cagcggccgc tttttttcag aacaggtccc tgtactcgaa cgctactata
    10981 ccgtgtatct agtagatagc cgaggacatg gtcgttcgac aaacgaagca tctatgttga
    11041 attttcagtt aatggccgag gatctgaata cgatcatgtt gctggaaaaa atcgatcagg
    11101 cagattttct tggatttagt gatggagcaa atctagcact ggtatttgcc agcagcttcc
    11161 ctaaaaaagt tcatcgattg attttaaatt ctggaaacac gcttgttaaa ggcgttcgtt
    11221 tttctgccag agtcatcagt aatattcatt atgcatgggt atggttactc tctttatttc
    11281 gcccaagtct tcgcaaaaat cttttagtta tcaaactttt gctgcatgac atcgggctta
    11341 cagaaaatga tttaaaaaaa atcaactccc ccactttgat catcgtggga aaaaaagatg
    11401 taatcaagct aaaacattct ttgtatatag ctaaaactat accaaaagct tcctttgtgc
    11461 ttgtgaaaga acaaggacat gaattagcta gaaaagatcc tgaacgcttc aatcgagaag
    11521 ttcttcaatt tctttctgaa acataactat taggaataga aggtgattta tttgttaaaa
    11581 aataccatac aatggctgaa aaaccaccta ggactgttca aaacaatctt tttgatttct
    11641 gtcattgtca ttattgtcgg tgaattgatg tccatcggta aaactctatc tatccagcaa
    11701 ttaggagaaa cgtttgccac tatacctgta tggaaaagcg gactaatgct attgattggt
    11761 cttatctctg tgttacctat ggttggttat gatattattt tgaatcggat gcttgaacaa
    11821 aagcagaatc ctcgttacct gtttgagaca agctggctga tcaatacgat caacaatatt
    11881 gccggtttcg gcgggtttgt tagtgtgggg cttcgctctg aactatatgg acaaaagaaa
    11941 gagagcagaa acgtgatcca agcattatcg aaagttttct tgtttttgat ggctggactt
    12001 tctatttaca gtctgctttc ttttttactt gtagtcttca cgccagttgc tccttttttg
    12061 aagcaatatt ggatatggtt gatcggcggc ggtctatatt ttcctgtcgt ctttttattt
    12121 actactttca aaaagaaagg attccttggt ggtacctcac ttaaaactca aggacagcta
    12181 ttgctagttt cattgttgga atggaccggt gtcctgttta gtttcctttc tgttggttat
    12241 ttaatggaaa tccggattga tctgtggcaa acaattccat tattcattgc tgcttcagtg
    12301 ataggaatcg tgtctatgat tccaggagaa attgggagtt tcgatgtaat gatgattatc
    12361 gggttgtccg ctattggtgt gccaagagaa atagtcgttg tgtggatctt gctctatcgc
    12421 ctgttctact acatcattcc gtttttgatt ggaatcgtct ttttcttcaa aaatatcggt
    12481 tccacttttg atcagcgtta ttctggtatc cctaaacaat tagcaacaga aattgcgcat
    12541 aaaatcgtgg ttgtcttatt atacttttct ggaatcatgc tagtactttc tgccactatc
    12601 ccgcaagctt ttacagagtt tcgctggctt catagtttga acccattgaa atttcatttt
    12661 attatccaat ttcctagtat actattggga tttcttttaa tcattatggg cagagggatc
    12721 gcagcacgtg tgaaacgagc atatttgcct acaatcttct tgattgttct agctttactt
    12781 tatgttttat taagtgattt tagtttcaca ccggtggttt tcttgtctat tctgcttctg
    12841 atcattcttg cttctaaaaa cgaattattt cgggaacaat tgatctattc atgggagtgg
    12901 cgtacgatcg atggaatcat tattggtgca ctgtccttgc tttacatcgt tattggggta
    12961 tacaatctgc cggatttccc tcatcggcgt caccatttta tttccttttt cttatttcct
    13021 tctgaaaaga tttggttttc gggattgttg gccatcatcg ctgttagttt catgatcgtt
    13081 ctatttgttc atttccttca aggagaaaag aaacagattg gcgaagcctt taatgaagaa
    13141 aaggccctga agatccttac aacttatgga ggaaactccg atagtcaatt gatcttctta
    13201 aaagataagc ggatgtttgc ctatgaaaaa gacggtgaac caactgttct tctccaattc
    13261 gcttgcttca ataataaatg tattgtaatg ggtgatcctt ctggtaagaa agaagatttt
    13321 ccagaagcaa tcgaagcttt tatcgaagaa acagatcgac tatgctattt accagttttc
    13381 tacgaaacaa gcgaagagat tgttatgatt ttgcatgaat tcggctacga tttcattaag
    13441 atgggggaag aagcttatgt agacctgaat agttttacaa cttcagggaa aaaaatgaag
    13501 ggcactcgtg ctgtcttgaa tcggatcgaa cgagaaggat ttacatttga tgtcttacaa
    13561 ccaccatttt ctactgagca aatgagtact ttcaaaaata tctctgataa ttggttgggt
    13621 tcacgaaagg aaaaagggtt ttctttaggc tttttctctg aagattatct acaaagagcg
    13681 cctattgcag ttgtcaaaaa tacccaagaa gaaattgtcg cttttgcaac gatcatgcct
    13741 acttacacga acaataaagt aggaacgatt gatctgatgc gatatgattc taaaaaagca
    13801 ccttctggca gtatggattt cttgttcttg catttgtttg cctatatgaa agaagaacat
    13861 atccaatggt tcaatttagg gatggcccct ctttcaaatg tcggaacttc cagaaagagt
    13921 tttttacagg aaaggattgc atacttggtt tacgagtttg gttctcattt ttattctttc
    13981 catggattaa gagaatataa aagcaagtac gctacaaact gggtctcacg ttatactcta
    14041 tattctagag atagttggat tgcatatgtt atgattgctt tgctgattat tgataatgcg
    14101 ccagttgagc agacacaagg tagaataagt ggtttgaaaa aatggatacg aagaaagtat
    14161 taaaaactgc ataccgatag atgaacactc tcatgatgaa taaaagagac tgaagtaaaa
    14221 cgaaagttca tgcttctaca atctcttctt atacaattga agaacaaaga gcggtccaaa
    14281 cagaaatcct gtttggaccg ctcttttatt ttaaacattt ttcattacca aaatattcga
    14341 gtcgcgtttc ttatgcttcg atctctgtaa cgatacctga acctactgtc cgtccgcctt
    14401 cacgaattga gaatgtcgtt ccgttttcaa cagcgattgg atggatcaag tcaacatcaa
    14461 tcgtcacgtt gtcaccaggc atgaccattt ctgtatcttc tggcaatgtg attgttcctg
    14521 taacatcagt tgtgcggaag tagaactgtg gacgatagtt gttgaagaat ggagtatgac
    14581 gtccaccttc ttcttttgtc aacacgtaca cttctgcttt gaattttgta tgtggagtaa
    14641 tggaacctgg tttagcaagt acttgtccac gttcgatatc gtcacgttgg atcccccgta
    14701 acaacacgcc tacgttatcc ccagcttccc cgtaatctaa cgttttacgg aacatttcta
    14761 cacctgttac aactgctttt tgtgtttcag gtttgatccc tacgatctct acctcatcac
    14821 cgacacgaac agcaccacga tcaatacgac ctgaggcaac agttcctcga cctgtaattg
    14881 agaagacgtc ttccactggc aatagtaatg gtttatctgt atcacgttct ggtgttggga
    14941 tatattcatc tactgtatcc atcaattcca taatagcagc ttcagcatct ggatcgcctt
    15001 gcaatgcttt taatgcggaa cctttgatca caggagtgtc atcgcctggg aaaccatatt
    15061 cgctcaataa ctcgcgaact tccatttcta ccaaatcgat caattcttca tcatcgacta
    15121 aatcaacttt gttcaagaaa acaatcaggt attttacacc aacttggcgt gacaataaaa
    15181 tgtgttcacg tgtttgaggc atcggaccgt cagttgcaga aacaactaag attgcaccat
    15241 ccatttgtgc tgcccctgta atcatgtttt tcacatagtc cgcgtgtcct ggtgcatcaa
    15301 tatgcgcgta gtggcgtttt tctgtttcat attctacgtg tgcggtattg atcgtaatcc
    15361 cacgttcacg ttcttctgga gctgcatcaa tactagcata gtcttgagga tttgctaggc
    15421 cttttttgcc taatactgta gtgatcgcag cagttaaagt agttttccca tggtcaacgt
    15481 gtccaatcgt accaatgtta acgtgtggtt tacttctgtc ataatgttct tttgccataa
    15541 tttacaaacc tcctaaataa tatagatcac tgaaaaattt cagctctcac ctttttatta
    15601 tagtcttttt ttgactaaat caaaaagaat acgtccgact tactttttgt aagattcgaa
    15661 ctaagcttat tatacgtcaa tattggtcaa agtcaactaa gagcactcgt ctgtcttata
    15721 cgcgaaaaaa gtacctttta taaataaaaa aatgtgtttt ttgagaaaaa cttcaagata
    15781 gtccttaact ttcttttgaa gcaataagaa caaaatgagg aaaagtttaa aattcagctg
    15841 ttgccaacaa ctttcaatag acgaaatctt ctatttcgtt aataataagg aaagaggtga
    15901 agtacatgaa aaaattaatt ggcgtacttg tcgttttagg catatttctc ggcggaagtt
    15961 ttgcagctta tcattatttt tacggcggcg aagcttatta tacaaaaatc acgacgaatg
    16021 gcgaaatgtc agctacccaa gcagataacg gagagaaatt cacgacttat acctacaagc
    16081 aagcggctta taataaagat ggtgaaaaaa aacaggtgac gcttcgcgaa gaaagagaac
    16141 atccattaaa gatgaatgcg tatcttaaac tcaaagtcaa tcctcgtaaa ggtgtattaa
    16201 gctgggaaga aataaaggct tctgaagtgc ctaaaaatgc ggcagaaaaa atcgataatt
    16261 agtaaatggt cttaaaattt tattagataa aa
//
LOCUS       DAZKOM010000060        16226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000060 DAZKOM010000000
VERSION     DAZKOM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.060"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..497)
                     /locus_tag="ACG8NF_001833"
     CDS             complement(<1..497)
                     /locus_tag="ACG8NF_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0472311.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(738..3083)
                     /locus_tag="ACG8NF_001834"
     CDS             complement(738..3083)
                     /locus_tag="ACG8NF_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0472312.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            3362..4264
                     /locus_tag="ACG8NF_001835"
     CDS             3362..4264
                     /locus_tag="ACG8NF_001835"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0472313.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4475..6250
                     /locus_tag="ACG8NF_001836"
     CDS             4475..6250
                     /locus_tag="ACG8NF_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0472314.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6316..7902)
                     /locus_tag="ACG8NF_001837"
     CDS             complement(6316..7902)
                     /locus_tag="ACG8NF_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0472315.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8153..8377
                     /locus_tag="ACG8NF_001838"
     CDS             8153..8377
                     /locus_tag="ACG8NF_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0472316.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8519..10270
                     /locus_tag="ACG8NF_001839"
     CDS             8519..10270
                     /locus_tag="ACG8NF_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0472317.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            10270..12054
                     /locus_tag="ACG8NF_001840"
     CDS             10270..12054
                     /locus_tag="ACG8NF_001840"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472318.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            12389..13241
                     /gene="licT"
                     /locus_tag="ACG8NF_001841"
                     /pseudo
     CDS             12389..13241
                     /gene="licT"
                     /locus_tag="ACG8NF_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            13338..15224
                     /locus_tag="ACG8NF_001842"
     CDS             13338..15224
                     /locus_tag="ACG8NF_001842"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0472319.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(15265..16167)
                     /locus_tag="ACG8NF_001843"
     CDS             complement(15265..16167)
                     /locus_tag="ACG8NF_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0472320.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         4965 a         2957 c         3341 g         4963 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctctggttt ttcacatttt tacataaatt tgtcttaata tcgtagatgt aagcttttga
      661 acaccattta ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt
      721 ctgattaatc tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct
      781 ctagcataag ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgcctacg
      841 ccgaatttaa aggcgctttt cttcggacca ttttttgccc aaagtgactc cactgttttt
      901 cctggcgttt ggatggattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac
      961 ccagatactt ttttcttcat cacatcggat gataagtcaa atttctcatc agtgccgaaa
     1021 atctcagggt ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt
     1081 attcttgctt ggctgtccat tccagtattg tcatcgtgtc ctgcccaact actgatagtg
     1141 atagatggag ttgagacgat cagccatgaa tctcggtatt cattggtaga accagttttt
     1201 ccgacccaat ctgcatttcc tagatttccg tttaagcttg agatgacatc tttgaatggt
     1261 gtagtgattt tttcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct
     1321 tcggaataaa tacggacaga attactttca tgtttataaa tcacattacc tgcattatct
     1381 gtgatggaat caatgatata gccttcttcg tagactccac catttgctaa ggcctggaat
     1441 ccatttgtct gttgaagcgt cgttacatct actgtcccta aaggagccga ttcaacgccc
     1501 caattatcat ttgctgggta gttcatcttc gccaagtagt gctcgtaagc gtattgtttc
     1561 gatccaccgg aattcaacac atcttgatat aagtgataag ctgggatatt gttggaccat
     1621 tccagtgact cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt
     1681 ttctcaccag cattttttcc ttcttgccaa gtcgttgcat aatctgatac acgggattct
     1741 gatccgacaa gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa
     1801 ccagcttgac gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccgacca
     1861 cccacgaagc catagatacg tccggttctg ttatccatca atacatttcc agtctccact
     1921 tggctaccag caccatcatc taacatgtag ccataatttt ctacgccggt ttgtaatgca
     1981 gcatagatat ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc
     2041 tgtgcttttt gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt
     2101 tctgccaatt gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat
     2161 tctctgtcag tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct
     2221 ttcgcttctt cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca
     2281 aaatcttttc tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtat
     2341 ggactatacg tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc
     2401 acatcctttg catctacacc aaagattcct ctagcagctt cctggactcc agcaatattt
     2461 tgccccttat tattccgacc aaatggtgag acattcaaat acgtagtaat gatctcattt
     2521 tttgaaaagt gtttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat
     2581 gttgtttcat cggttaggat ctgttgtttg actaattgct gtgtcaaagt ggatcctcca
     2641 gacgagccga tgccagtcgc ttctgaaact agtgctcgta aaacagcttt agggacataa
     2701 ccttgatgtt tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc
     2761 gaaatcttat cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct
     2821 gtattatctg cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt
     2881 tcttttttag tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt
     2941 ccgataccta gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga
     3001 aacactctaa aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt
     3061 tttcgagtat ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt
     3121 atagcaaata ataggacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg
     3181 aatgagataa aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta
     3241 gggaaagttt cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac
     3301 atcatcttat caatatacag gaattgttcg aaacaattgc ctttcaagga ggaatccatc
     3361 catgttatta ggctcacatg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga
     3421 agaagcagcc agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac
     3481 tagaagaaaa gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa
     3541 cggactaaca aatatcgtcg ttcatgcgcc ttatatcatt aatttgggaa atacgatcaa
     3601 accagaaatg tttccatttg ctgtacaatt tctgagagaa gagattgtcc gtgcggaagc
     3661 tttaggtgct actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagc
     3721 tgggattgct agaattattg aggggttgaa tgaagttttg acaaaagaac agacggcaca
     3781 gatcgctttg gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaatt
     3841 agcgcaaatc atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg
     3901 tcatacgaat gatgccggat ataatataaa agaagatttt tcaggtgtaa tggaagagtt
     3961 tgataaggtc atcggattag atcggcttaa agtactgcat atcaatgatt ctaaaaatcc
     4021 acaaggttct cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac
     4081 attgaacaaa atcgtccatg atccaatgtt tgacaacatt tcgaaaatat tagaaacccc
     4141 atatgtcggt aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tttccatgtt
     4201 caaagcgcaa gcattcgatc ccgaagtgtt ccctgagtta ttagcagaag aagtcacact
     4261 ttaagttttc tacctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg
     4321 tttaagaaac aaaggagtgc tttttgcatt cgttttcgcc atgctccttt tgccccctct
     4381 ttttaaattc agacaaacag ggtatactga tagaaaagta aaaaacgaaa agtttcacaa
     4441 acaagtagca aaacgtgatt gaaaggacga aaagatgctg aacattttaa accccttgat
     4501 cgaccaacta aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat
     4561 tattcggttg tatttatata gtaaagcaat ctatggaaca gatatgtcgg cgacctatga
     4621 tgatcagctg ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta
     4681 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
     4741 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
     4801 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
     4861 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
     4921 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
     4981 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
     5041 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
     5101 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
     5161 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
     5221 tcctatagtt ggcttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
     5281 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
     5341 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     5401 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     5461 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     5521 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     5581 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     5641 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     5701 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     5761 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     5821 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     5881 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     5941 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     6001 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     6061 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
     6121 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
     6181 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
     6241 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     6301 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
     6361 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     6421 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
     6481 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     6541 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     6601 gctgttggtc atcatcagta aaggaatcat cgaaaatggc aacgcaaaag ccaagaacac
     6661 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
     6721 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     6781 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
     6841 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
     6901 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
     6961 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     7021 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
     7081 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
     7141 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     7201 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     7261 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     7321 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     7381 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     7441 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     7501 gatgtctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ccaatgtctt
     7561 acttgtccgt gctcggattg cttgggccaa atccatttgg gtcacgattc ctaattttgc
     7621 tgccatatac tgtaaaagca tagcaattag actagacatc aaaataactg acatcaataa
     7681 atattggaaa ttttgtccac cagtgatcga agtggaccag ttacctggat ccatataacc
     7741 aactgctact aatgcacctg gtccagagta agcaagaagt gttttaaaaa aactttttcc
     7801 ctttggtaca tccactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     7861 aagtcgatgc ttatgaaatt gtttgttgga ttgattattc aagaaaaaac cacctttcct
     7921 atttatctac gattacattc tacacctcag aaagaaaaaa ttaaagaaaa agattcggta
     7981 tgcctaattt tttttcgtgg tgtgtcttgc tggttaatga aagaaatttt gttcttctct
     8041 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     8101 ttagtaaaat aaatcattga gagcaattcg gaaatagaaa ggagagattt atatggtatc
     8161 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     8221 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     8281 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
     8341 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaaa gactttttgc
     8401 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agactattca
     8461 gcaaaaaaga caagcacaga caatcctatg ctcttgataa agacaaagga ggagtaaaat
     8521 gtcgattttt agaaaattag gttggttttt tcgtcaggaa aagaaacatt atttaattgg
     8581 gattttctcc ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
     8641 catcattgat gagatcgccg ataagaattt atcaataaag atcatttcct ggtggatctt
     8701 attattgatc cttgcagctt tcttacaata tctttttcgt tatatctggc ggacgaatat
     8761 ttggggaagt gcagctcggt tggagagaga acttcggcag cagctattca aacattttac
     8821 agaaatggac aatctctttt accaaaaata tcggactgga gatttgatgg ctcatgcaac
     8881 gaatgatttg aatgcaatcc agaatgtagc aggagctgga atcttgacct ttgcagattc
     8941 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
     9001 actgattgcg ctgattcctc tgcctttgct agccgttact tcacgagtgc tggggtcaaa
     9061 actccacgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
     9121 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctcgc
     9181 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
     9241 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
     9301 tttaggtgga aagtatgtct tggaaggcac gatctcaatc gggcagatgg tctcatttat
     9361 tagctatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
     9421 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
     9481 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
     9541 ctcttttaac tatccgaaag aagaatcacc agcattagaa caaatcaaat ttgcgatcca
     9601 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga caatcatcaa
     9661 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
     9721 caaagaatat tcattgaatg ctttattagg atcaatcggt tatgtgcctc aggatcactt
     9781 cctgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
     9841 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattgccaaa
     9901 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacagag
     9961 gatttcgatt gctcgagcat tgatcaaaaa tcctgaattg ctgatcttag atgatgcact
    10021 ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    10081 agagaaaaca accatcattg cagctcaccg tttaagcagc gtgatgcatg caaaagaaat
    10141 cattgttgtc gatcaaggga aaatcattga acgaggaacc caccacgagc tgcttgcatt
    10201 aggtggctgg tatgaacgaa tgtgggaaaa acagcaatta gaagcgaaga tagaaggagg
    10261 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
    10321 cagaagaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
    10381 gttgctatct tatttgcgtt tgccctgtct gtgatcaatg tcctattgcc aaggatcatc
    10441 caaactttta tggatgatca tctggctaaa cagtcagcta cgacacaagt tattttgttt
    10501 tttgctggtg tgtacttgtt tggagtcata gtcaaaagta tcatttggtt tttccaatgg
    10561 tatttgtatt ccatggcttc attgaagacc tatcagtatg ttcgggtaag attatttgaa
    10621 aagctgcata cactaggtat gcgttatttt gaccagacac cggcaggatc gaccgtttct
    10681 cgagtgacga atgacacaga aacgctattt gaattttggt atgtcttttt gatggtattg
    10741 actgggatct ttgcagtcgt ttcttctttc gttgccatgt ttcaaatcaa tggagaaatt
    10801 gccttgtgga ctttggtgtt cttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    10861 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    10921 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    10981 aaagaatttg aacaagtgaa caacgagtat ttagacacgc gctatgcgat gatcaaaacg
    11041 aattcgattc tgttaagtcc aatcatcaat tttctctatg cattggcgat tgcgttggcg
    11101 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    11161 tttacgacct atgtccagga attttttaat ccaatgtctc aaatgatgga ctttttgagc
    11221 acctttacag atgggatggt tgccggtagt cgtattttga aaatcatgga tacagaagaa
    11281 tatgcaccgc aacaaaatcc tggagcagat caagcgatta cttcaggtaa gatcgagttt
    11341 cgcaatgtaa gcttttctta tgatggagag cacaatgtat tgaacaatat ctcttttgtc
    11401 gctaatccgg gtgagaccgt tgcattagtg gggcacaccg gaagcggaaa aagctcgatc
    11461 atcaacgtat tgatgcggtt ttacgaattt tatgaaggcc aaatcttgat tgatgatcat
    11521 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tgggattggt cttgcaagat
    11581 gccttcttgt tttatggaga tattgcagga aatatccgga tgatgaatcc agctatcaca
    11641 gacgaacaga ttcgccatgc agctgagttt gttcaagcaa acaagttcat cgatgaatta
    11701 ccaaaaggat atcactcaaa agtgatcgaa cacggtgcca gctattctag tggacaacga
    11761 cagctgatca cttttgcgcg aacaatcgtg acagatccta aaatactagt attggatgaa
    11821 gcaacagcaa atatcgatac tgaaaccgaa ggattgatcc aagaaggatt ggcaaaaatg
    11881 cgacaaggac ggacaacgat agcgattgcc catcggctgt ccacgattcg ggatgcagaa
    11941 ttgattttag ttttagacaa aggtcatata gtagagcgtg ggaatcatga aacacttcta
    12001 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtgt gtaaaaagag
    12061 gttgtgacag tagcatagta gcattttgcc ctaagcgcta gagtgaaaat aaaacagagc
    12121 ttcgcagatt cttcatctgt gagcgttagt ggcgtagtca cccgatttaa aaaagacgac
    12181 gacagctagt caaaagcggc taataaccac taagaaagcg ttgacaaaag cgtttgcttt
    12241 cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg
    12301 atgagtctcc catggttcca tggacggctt tttgggtttt tctttttgtt ttggatacag
    12361 ccctatatat catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt
    12421 cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt
    12481 caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac
    12541 aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt
    12601 agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga
    12661 ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg
    12721 aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc
    12781 catcgggaaa aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag
    12841 atgaagccgg atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccaacaag
    12901 acaataccta cctgttcact ataacaatac aggatattct aaatatcgtc cgctattact
    12961 ataagattac atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt
    13021 tttttctgac acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat
    13081 tgtttcaggt tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag
    13141 actttttagc cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga
    13201 tccatactgc gcgtttagtg catgagaatc gttgtaaata aataaaaaaa ggaggataaa
    13261 ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaata
    13321 aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg
    13381 tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt cgtttcaaat
    13441 taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt attgtaacgg
    13501 taatgcgagc gggagggcag tatcaagttg tcatcgggaa tcatgtgcca gtcgtgtttg
    13561 aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga
    13621 gaccgtttga tatgcttatt gatggaatat cgggctgttt ccaaccattc cttgctatcc
    13681 tagctgctga cgggatgatt cgtggactga cagcattcct tgtcttctta ggcgcgtttg
    13741 accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta ttccaattta
    13801 tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga
    13861 tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg agtggtgcag
    13921 cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct atttatcaaa
    13981 cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt cccattatct
    14041 ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta cctgagatga
    14101 tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta ggttttttac
    14161 tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt gaaacacttt
    14221 tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa attctggtga
    14281 tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct gtgaatggtt
    14341 ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca gcacttaccg
    14401 cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca gccattgttt
    14461 ctggtattgt ggggattaca gagccagcta tttatgggtt tacattgcca cgtaaaaaaa
    14521 tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt
    14581 taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat attagaccag
    14641 atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg atggtggtat
    14701 cctttgcgct gacttttttc ttttggaagg aggaagcaga tgaaacagat gatagacaga
    14761 aaagatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactgaatg
    14821 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
    14881 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gtccctgttt ccgacgaaac
    14941 atgcaatcgg gatggtttct gaagcaggtc tggaattgct gattcatgtc ggattggata
    15001 ctgttcagtt ggaagggaaa tattttgaaa gcttggtgca gcaaggagaa agagtgaaac
    15061 aaggcgatct gttgcttcgc ttcgacaaaa agaaaatcga agaggaagga tacagtttag
    15121 aaacacctgt gattgtcatc aattacatgg attatttgga tattttggaa aatcaagggg
    15181 aaacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaaaga
    15241 atggactagg cttaactatt ctttttagtc cacttcttta ttttcaaaat tgaaaggctc
    15301 tttcatgatg gctgagctga tagggcgttt atcaaatttt tgtcctacag aaattagatg
    15361 gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt ctagaagata
    15421 acaaatcgat gtattgatcg taaaattacc aattttcgaa tacagtcgtt cgcgtgtcgt
    15481 aagtcgcaaa gcggctgtac tcattgaaga tagcgttgaa tcaccgatac tggtcaaagc
    15541 taaaattggc acatgatttt gtttcaatat ttctgcggta cgaaggatca cattgttttc
    15601 acccgtataa gaaatcagaa tcccacacat atcacttgtg caattgtacg cttcatagaa
    15661 cgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca ttttcagcat
    15721 gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt tttttgcttg
    15781 aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa gtgtatcttg
    15841 aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac cgtctgtggt
    15901 ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg ctgtttcagc
    15961 taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg ctacacggat
    16021 gagtgtggac gggtggacat aacatcgctc agcaatctct tggatggtca tttcgccaag
    16081 tgctggagaa tcgcttaaga gaaactgaat cacttcattt tctgaaggag aaaaggagat
    16141 ttctttgatt ttttcacgta ataacatgta gataccttct tcttgttttt ttacgtaaat
    16201 cataactttt ttgaaaatga tttgta
//
LOCUS       DAZKOM010000061        16133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000061 DAZKOM010000000
VERSION     DAZKOM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.061"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..459)
                     /locus_tag="ACG8NF_001844"
     CDS             complement(<1..459)
                     /locus_tag="ACG8NF_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0472321.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLA"
     gene            complement(589..1299)
                     /gene="budA"
                     /locus_tag="ACG8NF_001845"
     CDS             complement(589..1299)
                     /gene="budA"
                     /locus_tag="ACG8NF_001845"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0472322.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1312..2976)
                     /gene="alsS"
                     /locus_tag="ACG8NF_001846"
     CDS             complement(1312..2976)
                     /gene="alsS"
                     /locus_tag="ACG8NF_001846"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0472323.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3181..3843)
                     /locus_tag="ACG8NF_001847"
     CDS             complement(3181..3843)
                     /locus_tag="ACG8NF_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0472324.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3853..4668)
                     /locus_tag="ACG8NF_001848"
     CDS             complement(3853..4668)
                     /locus_tag="ACG8NF_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472325.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4930..5373)
                     /locus_tag="ACG8NF_001849"
     CDS             complement(4930..5373)
                     /locus_tag="ACG8NF_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0472326.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5507..5845)
                     /locus_tag="ACG8NF_001850"
     CDS             complement(5507..5845)
                     /locus_tag="ACG8NF_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0472327.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5833..6210)
                     /locus_tag="ACG8NF_001851"
     CDS             complement(5833..6210)
                     /locus_tag="ACG8NF_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0472328.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6434..7627
                     /locus_tag="ACG8NF_001852"
     CDS             6434..7627
                     /locus_tag="ACG8NF_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0472329.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7793..8215)
                     /locus_tag="ACG8NF_001853"
     CDS             complement(7793..8215)
                     /locus_tag="ACG8NF_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472330.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8610..8804
                     /locus_tag="ACG8NF_001854"
     CDS             8610..8804
                     /locus_tag="ACG8NF_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472331.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8801..9295
                     /locus_tag="ACG8NF_001855"
     CDS             8801..9295
                     /locus_tag="ACG8NF_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472332.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9457..10629)
                     /locus_tag="ACG8NF_001856"
     CDS             complement(9457..10629)
                     /locus_tag="ACG8NF_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0472333.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10835..11650)
                     /locus_tag="ACG8NF_001857"
     CDS             complement(10835..11650)
                     /locus_tag="ACG8NF_001857"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0472334.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11800..12246)
                     /locus_tag="ACG8NF_001858"
     CDS             complement(11800..12246)
                     /locus_tag="ACG8NF_001858"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0472335.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12243..12758)
                     /locus_tag="ACG8NF_001859"
     CDS             complement(12243..12758)
                     /locus_tag="ACG8NF_001859"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0472336.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(12831..13745)
                     /locus_tag="ACG8NF_001860"
                     /pseudo
     CDS             complement(12831..13745)
                     /locus_tag="ACG8NF_001860"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(13742..16069)
                     /locus_tag="ACG8NF_001861"
     CDS             complement(13742..16069)
                     /locus_tag="ACG8NF_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0472337.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4607 a         3236 c         2861 g         5429 t
ORIGIN      
        1 tgctaataaa agatctacat atttttctag aaatgcttga tcgatttcat catacccttt
       61 cgttactccg caatcgatat ctaaaacacc gattaaatta ccttctttta ccattggcac
      121 tacgatttct gattgtgcgg ctgaatcgca ggaaatgtaa tttgcatggg ttttgacatc
      181 atcaacgatc aatgtttctt gtttttcagc tgattctcca caaactcctt ttcccatctt
      241 gatacgtgtg caagaaactc gcccttggaa cggtcctaaa atcaattcat taccatcgaa
      301 taaataatat cctgcaaaga cagtctcagg caaagtctca ttcaacaatg cagatgaatt
      361 tgctaaattt gctaaccagt tatcttctgc ttctaataat ccttgttgct gtaataataa
      421 aatttcgtat gcatcttttt tctgtttttc tgtccacatg ataaaaaccg ctcctttttg
      481 atctatggag aagattatag ctcagttttt ttgtaaacgc taggtttcct cctcaaaaaa
      541 acgagatacg tcaggttaat caccttgaac gcatctcgct gtctttttct attctgcttg
      601 ttggatttct gctgctaaat cagcataatc aatcgtttcg tgaacaaaag tcggatcatc
      661 aactggaaaa tgctgtacaa gggacgagat gcggctaatc tcaacttttc cttcagccac
      721 ttcaaaatcc attacatgtc cgccaaatgt atgtgtatca tcgataaaat gcaagtgaaa
      781 tcccgctgca gcaactcctt ggaataattc aggcgtaaag aacccgacta atgttccttg
      841 gacattgctt tgttggaatt ctggttgaga ctctgaaatt tttgctagtc ttctgtaagg
      901 tttttcttgc ttcggcatga tacggatgtg catatttttg aataatccag ttatcttcac
      961 tgccaaaaat aggttttccc cggcttctct tactaatatg tcttttttca attgttctcc
     1021 atctgcagtt tttgagacag aaaaactagt atctggcgta aaatctgtaa ttgctgcata
     1081 gggggtaagt tcacttccat ctaattgggc aactgagcca tctgatcttc cttgataagc
     1141 tacgccatct aaaaagatga cttcaccatc aagcccatgc aatgttccca atcccagagt
     1201 tcctttttct aatatatgag caatcgtttc tgttccatcc atcaagccag ccatcaaagc
     1261 tcctaatgtc ccgtgctggt ataagatttt ttcagtcatg tcgacacttc cttaatagaa
     1321 ttgatctggt aagattgttt ctcccaattt ctcattatca cggtaatcaa tcggaatatc
     1381 aataattacg ggtccttctg tcgcaaatcc ttcttctaaa gctttttcta attcctctac
     1441 acttgtagct cggattcctt tagcgccaaa agcttctgca tatttcacaa aatcaactgg
     1501 accaaagtct acacctgacg cccgctgata tttcattttt tcttggaact ctaccatatt
     1561 gtagtgtcca tcattccaaa tcaaatgaac gatattcaat tttttacgta ccgccgtttc
     1621 taaatcctgg gcagagaata aaaatccccc atcgccggaa actgatacga tttgtgtatt
     1681 tggacgcacc aatgcagcag aaatcgccca tggcaatgca acaccaagcg tttgcatacc
     1741 attactgaat aacaaatgac gaggttcata acttctaaag tggcgtgcca tccaaatata
     1801 atgactacct acatctacgg taacagtcat atcatctgtt acttttgact gaagcgtgtt
     1861 aataacttct aaaggatgca agatacctgc ttctcctttt gattgaacaa tgtcccgctc
     1921 cgttaattct gcttgaagag atgctaaata ttcttttgca tcttctgaca cttgttgagg
     1981 ctctaatgaa cctgtcaata aatcaagagt ggctgagata tcaccgatca attctctttc
     2041 aggctgcata aaaggatcga tttcagctgg cgcttcatcg atcacaatga tccgcgcatc
     2101 tttttccgca ttccagtttc ttgcttcata ttcgattgga tcatagccaa tagcaataac
     2161 tagatcactt cgtttcaaaa gcatatctcc tggttggttt ctaaataaac ccacacgacc
     2221 aaagaaatgc gcttctaatt cacgtgagat cactccagct gcttggaatg tctctacaac
     2281 aggcaatgct gtctttccaa ctaattgcct gatggctaac gtttcttttt cacttgatcc
     2341 tcgcatacct actaatagta ccggtagttt tgcttctcta atgcgttcag ctaggtaacg
     2401 gatatcatct gcagaagcag aacctaattt aggatcgctc aatggcttga tgacattccc
     2461 ttggacaggt gcatccacta catcttgcgg gatactgata aagctcgcgc ctttttttga
     2521 actcttagcc atacgataag cattggcaat cacttctgaa attgtttctg gatcttggat
     2581 ttctgcactg tattttgtga ttggctggaa cagtgctgca ttatccatgc tttgatgggt
     2641 caacttcaat agatcgcttc gttttacttg tccggcaatt gctaaaactg ggtcaccttc
     2701 tgctgtcgct gttaccaaac ctgtagctaa gttactagca ccaggaccac ttgttgcgat
     2761 aactactcct ggctctcctg taatacgtcc gattgcttgt gccataaatg cagcattttg
     2821 ttcgtgacga gtcacgatta attcaggacc ttgatcctct aattcattaa atacaccatc
     2881 gatttttgca cctggtatac cgaatacata atcaacatga tgattaatca aactttctac
     2941 tacagatttc gaaccttgtt tcattttttc gtccattgaa catcgtcctt tttatgcttt
     3001 atttttaatt attagttaga aataatcaca aataattgat acagtttcta aacctgtttc
     3061 attcaaaagt atagcacgtt ttatttcatt tgtttagcca tatgtttcaa aaaatgaaaa
     3121 agaatgacag tttttacata aaaactgtca ttcttaattg aaagtttcaa tttataaagt
     3181 ctacagatcc atagttctca tcaatttacg attgttgaag taaatcaaac caaatacgat
     3241 agaaggacct agtagaatac accattgggg ctttacgaaa aagaagagaa acaaacttgt
     3301 caatagtaaa aaatctagta aactcacata ccattttttc aacgtatagt agaacgctat
     3361 ccgtagaact tgatttggtt tcgacgctgg atttctcacc tgaaaatacc agtagttgat
     3421 gctaaaggaa actgataaac aagcaagtaa aacaaacaac ggaatcaacc attttccata
     3481 agaaaaacgt ataaaaaaga aacaatcaaa aatcattatc acactaacta tccaagcaaa
     3541 tagccaataa aaaaatccct ttagccaaaa ttggcgaaac gtctttatga acaaacgtac
     3601 tggtgaaaaa cttttttctt ttgctaattc ttctgtcata cctatcatca cgatgcaacc
     3661 tggaccaata aaaagtaaag aaaaaataaa ggaaaagaca ttccttccat ctatcgacaa
     3721 aaggaacgca gctgctatat acaagatatt gacacaccaa aaacaaaggc tacttgccaa
     3781 tgattgatac aaccaataaa agaattccat ataaacattt ttgtcaaaac gctgctcttt
     3841 cataatcacc tcctattcct tcattcctga tgaagcgatc ccttctacaa aatatttttg
     3901 catcgctaaa aatataaaag caacaggaag aacagaaaca actgaggcag ccatgattaa
     3961 tgcgtactct gcatcaaata gcccaataaa cattttcaac cctaactgga ttgttttatt
     4021 ttccgtagaa gacaaataaa tgaatggccc catataatcg ttccatgtat taacgaatgt
     4081 gatgatcgtc aaactggcaa tcgccggttt tgtaagaggt aaaatgatct tgcgatagat
     4141 tccccactct gataagccat cgatccgcgc actctcacat aaagaatctg ggatactact
     4201 gtagtattgc ttcaacaaga acacaccaaa agcattgaac gcttgtaata gcaccaacga
     4261 ccacaacgta tatgttaaac ccacttttct catcataatg aactgcggga tcatataaga
     4321 ctgccaaggg acagcaatcg tgccgatata tgctaaaaat aaaatatcgc gccctttaaa
     4381 atttagttta gaaaacccgt aagcagcaaa actagaagta aacaactgaa tcaacgtgat
     4441 cacaacgctt aaaaaagctg tattcttaaa aaatgtcact aaaggaatac gagaccaaat
     4501 aaccagataa ttctctaaat gccaagtttt agggatccat tgaatcggaa tcgtgaacac
     4561 ttcattattt gctttgaatg aagcagaaac catccataaa aaaggaatga tcgtcaataa
     4621 agctaataca atcagcagaa tcatacccaa ccattgaata aagggcatgt tttttgaacg
     4681 aatttttgct ttttttcttt catttcgaac acctccaaag ttataatctt catttctttg
     4741 taatgattac aatccagaac aacggttatt atagctgatt catttccgtt cttcttatgt
     4801 ttgtaagcgc ttatttaaca agttatgtaa atctctttat tttttttgat ttaaaaatga
     4861 aaaaatacaa aagcaagact gtcatacgtc tcgcttttgt attgaaattt cttcaattag
     4921 agatacttat tactgttcaa aagcttcttt gaaacggtga atcaattcat tgactacagg
     4981 tgataaaatc cgattctttt tccagactag catgcagttc gtttcaatca ttgggtcaag
     5041 cggaacgaat gtcactcgct ttgaaagatg acttacagca ccttctatga ctaaagcagc
     5101 acctacctga ttctcaatta gcgcaaatac attgaaaatc aaattgtacg ttcctgtgat
     5161 cagcaactgc tcctttttta tgcctaacca gtcagcaaac attcgctgta tttctggacg
     5221 ctttgagatc aaaagaggta cattcgcaaa gtcttctttt gttatcacgt ctttttttgc
     5281 taaaacagaa ttttctaaaa tgagcaatcc ccatttttcc tttcttggta acaaaatcgt
     5341 ttcgtatttc tctgtatcta ctggatctag caaaatcgct aaatctaaca ggcttttttc
     5401 tagtcactcc gtgatatcat cacttgttcc gctgtggata tgaaattgaa cgctgggata
     5461 atcacggatc aacttttcta gtaatagtgc caatgtattc gaattatcag cttccacaca
     5521 gccgatcgta aatcggccgc tgaataattg tttttttctg ttctcgaatt cttgagtggt
     5581 ttgattcgtt aaatctaaaa tttcttctgc tcggcttttt tagaaaataa cctgcttctg
     5641 tcaagatcat tttgcggttt tcgcgtacaa acagttctgt tcctagttcc tcttctaact
     5701 ccttcagttg tcggcttaag gttggttgtg taagatgtag aacttctgcg gcttgagaga
     5761 ttgttccttc ttctgcaatt gtccaaaaat attttagtaa gcgtaactcc atagcgatgc
     5821 tccttgattt ttttacttga tacattccat tttatttcta gataacacac gatcatattt
     5881 cttgattttt ccttgtaaac gttccaaaca ctcttgcatt tcagcaattt ctttcgtcaa
     5941 aagttcactt tcttcaatca aaagcttacg ccgctcaaat acagttgctt ctccttcgct
     6001 agataggcga atatattcaa tcaaagactc gatcgataat ccggcatttc gcatacagat
     6061 cgtaaagtcg acccatttta ggtcttcttc tgtataatca cggattccgc cttttgtccg
     6121 attaactggt ggaatcaacc cgattcgttc ataatagcgc aatgtatcgg ccgaaatgcc
     6181 cttttcttca ctgacttgtt tgatattcat ctgatcactc tttctcttaa tttcttgcca
     6241 ctttacttat gtattttatt tattctacaa ctttaagtgt actccaagtc aatactcatt
     6301 tctattaaaa ctaagctgga ttatctatat cggttgtttg tgtattattt caaataaaaa
     6361 agtcttgttt ttttattctt ttttggatat acttttaaca atttagggaa attttaggaa
     6421 aaggggtata aaaatgacaa cgtatcagcg aaaggtatta ttatcgacat cggcgggaat
     6481 tgctttagag aatatggata ttatgttttt agctttttca ttatcttcaa tgattacttc
     6541 ttttcatatt agtggtacgc aagcgggttt catttcaacg atcactaatt tggggatgct
     6601 cattggaggt attttttttg gcatcatagc tgataaatat ggacgtgtga aagtattttc
     6661 tcaaacggtc cttctgtttt cgattgcttc tttattgatg tattttgctt caaacatcta
     6721 cttggtttat ctctttcgct ttatcgcagg tgtaggagcg ggaggagaat acggggcgtg
     6781 tatgtccttg atttccgaaa gtttttcaaa aaaacaaatt ggacgagctt cttcggttgc
     6841 tggtattggt gcgcaggtcg gtgcggcttt ggctgcgatt ttagctgctg tgatcattcc
     6901 aacgtttggt tggaagatgt tgtatgtcgt aggtgtactg ccggtgttga tggtcttgtt
     6961 cattcgtcgc ggcttgactg aaccaaagga attcaaatct gtacaatctg ccgaaaagaa
     7021 aaccaaactt agcagactgt ttgaatcaaa acatcttgct tggcaaacat gtggccttag
     7081 tatcatggta actgtccaaa ttgccggcta ttttgggtta atgaattggc tgccttcaat
     7141 tatgcaaaat cgattagggc tttcggtttc tgggtcttca ttgtggatgg tcagtactat
     7201 tttaggcatg tctttgggga tgatgacttt tggcataatc atggataaat tggggcctaa
     7261 actagccttt tctttgtttt taatttgttc atccttatct gtctatctac ttgtgttagc
     7321 ccatagccag tggagtttgg tagcagccgc tgtgatagtc ggctacttta tcaatggcat
     7381 gtatggtgga tatggcgcag tgattagtag tttgtatcca acagaaatcc gcgcgacggc
     7441 gaataatttc attatgaacc ttggacgagc tgttggtggt ttttcttcta tcgtcatcgg
     7501 tttcttgatg gatcattata gtttaactgc cgtaatcttg tttttgagcg gtatttacct
     7561 agtcagtctg ctggtcttga caactcttac tggtgttaca gcattaaggg aaagcttgca
     7621 gcattgatag ttgagtctgt aaatgacaag aaagaggtgt ggcaaaagct tgcatctgcg
     7681 gtaataagac cagccaaatc aaaaacatga gacactgttt ccgtttggct ggtcgtatta
     7741 cttgatgcag ggcagctttt tcaactgcta atttccttct atctggcggc gactattctt
     7801 tgacgtccgt catttcaaag tactttacta ataacttagc ataacgaaaa actgtggata
     7861 cactcgtata ttgagcgtct gcataatctt ccaaattttt ctcaggatgt tgatacatga
     7921 attcaatcaa atgataatta tatgattttc tgtagaaatg aagataaata atgtagattg
     7981 ggaagttctc ctgcttttcc atagaaaagc gccgattatt ctcattcttc aaaatgacct
     8041 tatcaggata tttttggaac aattcttcta aatctgttag ataattcaag atagacttac
     8101 ttgatacctt caattcgttt tgtagttctt ctattgaaca ccaacgttct gtttcaagca
     8161 gtaaatgaat cattttaatt ttcttttgag aaacttttgt taaaaaagtc tgcatatttt
     8221 ctcctcctgt atctacttta ctatactaca tacaagaaaa aaaacggata caaatatcat
     8281 attgtcacac cgtcaatagt gcataaacct gttattattt ctcttttttt cctaagtatt
     8341 ctattgatat gaatcattta atcgtttatt atataaatgt caataatcta ataataaaat
     8401 aacgtacata ttttattatt gcatacttaa taacagaact atagcaaaaa atcccgtgta
     8461 tcaacacggg attagattaa gtttcctcaa tcactattca taacacaatt attataacaa
     8521 gtaagtaaac aattgaaaag gacatttatt gaaaacggtt gttttttact taaggaggaa
     8581 atttagcgaa aaaattagtt gcattgtttt tggtcgcagg aagtttgttg agtgggtgtg
     8641 gtgctactac gtctactact caaagcacaa gttcagatca ggcttccagc agcagtacca
     8701 cgatctccga aagtacggca gattcatcga aagaacaaga acaaattgaa aagaaaaaag
     8761 aagagaaaca aaaagcggaa aaagctgcac tagaaaaaaa gtgacagaag cggaatctgc
     8821 tttgtcacaa gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcagctat
     8881 ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa ttgattccac
     8941 aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca
     9001 agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg cgaggcaaga
     9061 agcaatcgca cagcaacaag cagaacaggc gcgacagcaa tcagcagctg aacaggcctc
     9121 tcagcaggca caagccgcag ctcctgagca aaataatgaa caaactgtct atgtcacacc
     9181 gaccggttca aaataccata cacataaatg tggaaatggt acgtataccc ctgccacttt
     9241 aagcgaagct caagctcgtg gtttaacccc ttgctccaaa tgttatggaa actaattcaa
     9301 aagttatact ataataataa aattcttctg aaacataaaa aaatagaaga tggcgttcca
     9361 gatagtaaac caaaaataac agtttacacg tccatttcgt cagcttctct cttttttcat
     9421 ttagtctgtt ccctctgctt ttttggacat cttcattcat tctttctcaa tcaaatagta
     9481 cacctctttt tcttttcgca gtttatatct tccttttttc aatttcattg aaaactgttt
     9541 cgaatgttta tgttttacat ggattttaag caggagctcc tcatgttgca agcgatagtt
     9601 cctgcctttt aaatcagaaa atctcactag tccgtattca tcagaagctt tcacaaatgg
     9661 aactttttct ctagtaatat agtgtttgcc gttgagcgtt tttacagaaa agaccattcc
     9721 ttcgattggt cgtccgtttg tgttctttac ataaaaagat aaaggatagt ttcgcttagc
     9781 gatggccaaa gaatctaaat aagcgatgag tagccaaact aagccgattg atccaagtag
     9841 tataccgata aaccaaataa aaaatttcca ttttttccat tgatcaattc cttggatcgc
     9901 tttttttgcc tcttttgctt gataaggaat acgatgcccc gtcacaagca gtcgatgtgt
     9961 attgaccata taaggagtac aggtaagcaa agtcacgaga tctctgcctt tttggatacc
    10021 caaagaatct acttcatcag gtaagaccac ttggattttt tctacttgat acgccaatgt
    10081 ctttccattg atctggatga aaaactggtc tccttttttc aacttaggca aatctgtaaa
    10141 taattttgct tctgggagtc cacgatgccc agaaagaacg gcatgggtgt ttttcccgcc
    10201 agtcggataa gaactttctt ccaataatga cgttcctttt tgcaaaaata tttccgttgt
    10261 ctgatcgaat ataggtaagc tgacattgat ttttgggatt tcgatgactc caagcatatg
    10321 ttccataaaa aaggcattac taggtaaatc ttttaatgct tttgcatcaa ctgcttggtt
    10381 gaaactgcct atccctggtg atacgttttg ctctgccaac tgttggtttt tctttgtcat
    10441 tttttcctgc tgttttttta tttcctctga attcttattc gatgcttgct tttgataatg
    10501 gatgatcatt tgctgggcaa gaaaatcatt cagtgaatct tctacaaaag gataagaaaa
    10561 gatcccgatc ccaacgacta aaatgattgc catgaagatg tccagtattc ttattctgct
    10621 tttattcatc atgtccacct attttctaat atgtatatga agaggttgtg aaatcagcgc
    10681 cttgacctga gtattagcga agcaaacggc aaaatagttc ctcctatttt gccgtttgtt
    10741 gagcgaatac caagagaccg accacttgtc acaacctttc tccccatttg aatcactatc
    10801 gtttatttat cgtatgattt ttgtttccaa tagtttaagc ttctgtttct tgatggcgtt
    10861 ttttgaaata gatcactgct cctccaacta aagcagtacc taccaagatg aatacgatga
    10921 tccccatacc accagttgaa ggcaagattc ctttaggttg gttatcgatc gttgtttcat
    10981 cagaagttgt tggatcatca tctactgtaa attcagtgta cgcagcttca ttcttaggta
    11041 atgcgtagcc ttctggtgct tttgtttctt gaaggcagta tgccccttgt ttcagccctt
    11101 cgattgaaaa tttcccttct gaatcagaaa tcaatttcac ttcttcccag tcagaacctg
    11161 tgacttcatc tgaccaagca tatgggtatt ctcctgaggc aaggtttgct atttttttca
    11221 ttaatttaac agttggatct tttgctctta gaatagaaaa ttctgcatcg gctaattttt
    11281 cttgcgtgtt cacatcttgt ttgatgaagt ggaaatcacc gtagttttgg acattttcta
    11341 cagtaatcgt gttatccgta gttgtatttt cccgattgac tacaccgttt ggattcccaa
    11401 gattggcttc atctgcgcca tggatgatac cggttgtttg ttgcaatttt ccgtttttta
    11461 ctataaaagt agtttctttt tgtggattgg ttaattgata accatctggt gcttttgttt
    11521 caactaaagt ataattgccg tctattaatc cattgacact gatggatcct tcagtatcag
    11581 ttgtgaatgt caacgctgac tcagcaggat catagagagc gacaccagtg ttttcatcaa
    11641 aaccggtcac gaattgattc tctgaatttt tgatctggaa ttgcgcccct gctaacttat
    11701 ctacccctga aaagttccca gctttctgga ttttgatatt gctttctttg atgacatttt
    11761 tagcatatag gtgaatcgtg tctaaaggtt tgtcagggtc tagggtaagc ttaacacgat
    11821 atttggcgac gtttcgacac cgggctttgg tgtttctaca aagacgtata cagcatcttt
    11881 cccatcagat ttacctggaa gagtcaggtt agctaatcct tcaccagaag tcgttacact
    11941 tgcgaagggt gtagcgtctg ctggaactgc tgtgtcttta cttggatagt acgtagcagt
    12001 gatgtcatat acgttgaatt caacgccgtt caacggttcg ctattcggaa atgtcaattc
    12061 cgttccgttg tttatttgat tttctggtgc tgaagtgaat ttcaatttat gcaaattaac
    12121 cgtaacattt tctggtgtag gtgatacggc tgcagaaacc tctgcagttc ctaggccgag
    12181 aacgaaagga agcagcatga tagcagttcc taaaaacgtc catatttttg ttttgatgtt
    12241 tttcatttta tttccctcct attgtttcct ttttgttgtt ttttataaaa ccaagctgtc
    12301 cctgttgaaa ggactagcaa taaccctaaa attgagaaac ctccaagcat ctcttcgctt
    12361 gtttttggaa agcttcctcc atttttttca tttgatggac catttgtctg catctgaact
    12421 ggcgtttttc cattattcgt cggatttgct tttggttcgt tgatgatttc caatggtata
    12481 ggatgaagcg gatcataact gcctttttca acttcaaaga gaatggttga ttgactcaat
    12541 atatatcctt taggcggttt aatctccact agttcgtaac ttccaaaaga caatccatgg
    12601 acagaaaatg ttccatcttt actggaagta aaaatcgttg ctcttgaatc atctttggct
    12661 acccaatgat ttatcccgtt ttcttgcgct aagtattggt catttttttt gactagaaat
    12721 tgagcatctg ctagtttttg tttcgtttgc cgatccactt tgacaaattg tttgcctcct
    12781 gtttggatgg caagatgtgt ttctactgtt tgattgtcag tcgtcaaacg tacatcgtta
    12841 tcaaatgtcg ttttttcact gttttctttc aatgtcattt ggtaacggat cgttaatttt
    12901 tttccagcaa acgaactaag atcttttcca gaatctgata agacaaaacc atgttttatt
    12961 ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca aaaaatcgat cgtttctggt
    13021 tctagccata actgaggatc agctgtatct ttgatcgtat aggttcctat ctcgttgatg
    13081 ttctcaggaa ttgttacggt gatgagataa gggactttat ccccatctgc aaaatctgtc
    13141 tgcttattct ctactgtttt tgttaatgtg agtgcagcag gtattttttg ttcgctcttt
    13201 ggatacaagt ggatggtcgt caatttatga ttagatgaat ctgtcacagg aagcacaacg
    13261 acaaatggtg ctgatttttc ttttacttaa tctgaagtct ttatttccgt aaatcggtaa
    13321 acagcgtcac gaccttgctc atctttgtcc gatgcactaa aagaagcaat gccctcttcc
    13381 tcgtctactg tttttgtcac attttctgcc agtatttttt cactatcact tttttgtgct
    13441 agttttcttt gtgcgtcttc cacactactt ccttctgacc gcagcttgta aaattcatcc
    13501 gagacatcat atatagcgaa agtaacgccg ttcaaccctt ttactccttg aaagttatcc
    13561 gggatattta ttccgtcgtt ttgactgata cttggcatct tatcgctttc gaacattcga
    13621 ttatgcaaag taaatgtaat aggtgattct ctagctgaca cagtcggaga acctataaaa
    13681 aagaccagta ggataaaaaa gagaacagca aatatatcca cacgtacatt ttttgttttt
    13741 ttcatcgctg ttcacctgct tttttcttac gatggataaa ataaaagaag ccagaaaaaa
    13801 taaagatcag tcctaaaaag acaaagggca gtagacctat cccacctgtc gaaggaagca
    13861 agagagtcgg attgatcgtc aacttagcgg tatctgttgc gataatcgta taattattgt
    13921 ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga tccaatatct gtccggctgt
    13981 taactgttag ggtataatcc aatgattcgc ctaagacaac accggtaacg tggagatttc
    14041 caaccgaaat cgagtgactt tgggcatcat aattaaaggc agcgtcatcg acagtaatca
    14101 ctgcgttagc tttatagatg gtataagttc ctgatgtgtt ggctatatca gtaaatgaca
    14161 acttgggatt tgctgctttg actttgtcta ttccgctatt cgttagttta attgtatagc
    14221 tgccagcatt aaccatattt tttgctgatt gtgcatctgc tgtgtaatcc atactgctaa
    14281 gtgccacttg tactggagta agcgaactag tgacaccctc tgctagcttg acatggattg
    14341 acgggacacc tgcttttcca tcatatgtct tactgccact gagctctacc gcaccgctga
    14401 tttgcttaat cgtatagaca tcagatgtct gcacgatagt aaataaatag tttgttctgg
    14461 cagctagttt agctagtccg ctttttgtca attgccaaga cgtataggtt ccaatttcag
    14521 taggcgcagt tacttcaact cccttttcat tcatcgcttt gaaatcagtt ggattccatg
    14581 tgatttgatc aatactgaga tcattgttta aaagggatgt tgattctgtt ttatcattaa
    14641 aggcatattt aacgatcggt acaacagaaa gaccatcata gtacttactg ccatttgcat
    14701 tgattgatac tgttgcattt gtcttataat ataggatgac ttcagaaatc tgttttccat
    14761 ttgcgtcctt tacgattaca tttccatttg tatctaaact agctaaaaca ctcattgctg
    14821 attggttctt tgtttctatc gctgaaacag ctgtaaaggt atatccgttg atagtaggcg
    14881 tattagtcgt caggtcttgt gcagcacttc cgtaagtaag attcattatt ttagaaggag
    14941 caatcatctg tccaaaatag tcttgatact taatagtgat tggtgtttgt ttgcctgtat
    15001 aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga accgtttgtt tgtgcaaaag
    15061 aatagcccgt tactgctgtt ggagtgataa ccactttgtc acccacatgg attccagata
    15121 atgtagtccc gtacgttccg atttttgctg ctccaccatt gactgtataa gaaaaagttc
    15181 ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata ggttggaaaa tttgaaacat
    15241 ttcccttacc atcaacgatc tgcccatttt tgatcgagta ccccgtgaat ggaatatcat
    15301 ttgctaactg tataccgatt gaaaaagtac tcatacctcc tccggcagga agccctcccc
    15361 atgttgaggg tgtaaaagaa taacttttat ccgcattggg tgtaactgaa tcgatcgtat
    15421 ttccagcggt agccaccccc atatagatac tttttgggaa tgtgatagta gtaccattta
    15481 ctgtggagcc aataaacaga ttatggatat ctggacggtt atcaataaaa gcgattgctt
    15541 tggttacgcc ttgtaacgca gttttatccg cactcatcga tccgttaacc gcaacaatga
    15601 ctccatcttt ggcatatatc actgtatatc catctggtat ggtatgtccg ttgaaattcg
    15661 cagtggccgc aacagtacta aatgtctggc ttctacctgt ttctgaataa gctttctccc
    15721 ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc aatcagttga gtcgaggatg
    15781 tggttgaggt tgtctcgttg ttgctagact gattggtgct ttctatggac tgtatttcag
    15841 aatagggtgg actatttgtc tcctctgtcg tttggttagc aaaatttgac gtttctgtat
    15901 aaacgtttac ataaatactt gataagctcc ctaagaaagt aaaaatcaag atgatcgaaa
    15961 tcataatggg atgtaagtat tttcttttag atacagcttt gagtctagaa acaaaatgtt
    16021 tttgacatat acgttggatt ttattttgag ttttcttaat cattttcatc cctcctctca
    16081 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt att
//
LOCUS       DAZKOM010000062        16034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000062 DAZKOM010000000
VERSION     DAZKOM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.062"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8NF_001862"
     CDS             226..894
                     /locus_tag="ACG8NF_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0472338.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8NF_001863"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8NF_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472339.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8NF_001864"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8NF_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0472340.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8NF_001865"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8NF_001865"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0472341.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8NF_001866"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8NF_001866"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0472342.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8NF_001867"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8NF_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0472343.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG8NF_001868"
     CDS             6859..7764
                     /locus_tag="ACG8NF_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0472344.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8NF_001869"
     CDS             7855..8532
                     /locus_tag="ACG8NF_001869"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0472345.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8NF_001870"
     CDS             8529..8699
                     /locus_tag="ACG8NF_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472346.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8NF_001871"
     CDS             8813..9709
                     /locus_tag="ACG8NF_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0472347.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8NF_001872"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8NF_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0472348.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8NF_001873"
     CDS             11372..12118
                     /locus_tag="ACG8NF_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472349.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8NF_001874"
     CDS             12115..12954
                     /locus_tag="ACG8NF_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0472350.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8NF_001875"
     CDS             12955..13905
                     /locus_tag="ACG8NF_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0472351.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8NF_001876"
     CDS             14046..14798
                     /locus_tag="ACG8NF_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472352.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8NF_001877"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8NF_001877"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0472353.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5198 a         3001 c         3229 g         4606 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgt
//
LOCUS       DAZKOM010000063        15743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000063 DAZKOM010000000
VERSION     DAZKOM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.063"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8NF_001878"
     CDS             complement(24..722)
                     /locus_tag="ACG8NF_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0472354.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8NF_001879"
     CDS             complement(715..2067)
                     /locus_tag="ACG8NF_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0472355.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8NF_001880"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8NF_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0472356.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8NF_001881"
     CDS             3851..4588
                     /locus_tag="ACG8NF_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0472357.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8NF_001882"
     CDS             4713..5192
                     /locus_tag="ACG8NF_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0472358.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8NF_001883"
     CDS             5189..5956
                     /locus_tag="ACG8NF_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0472359.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8NF_001884"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8NF_001884"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0472360.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8NF_001885"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8NF_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0472361.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8NF_001886"
     CDS             7567..7797
                     /locus_tag="ACG8NF_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472362.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8NF_001887"
     CDS             7788..8036
                     /locus_tag="ACG8NF_001887"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472363.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8NF_001888"
     CDS             8142..8726
                     /locus_tag="ACG8NF_001888"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0472364.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8NF_001889"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8NF_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0472365.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8NF_001890"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8NF_001890"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0472366.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8NF_001891"
     CDS             10627..10806
                     /locus_tag="ACG8NF_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472367.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8NF_001892"
     CDS             10828..11853
                     /locus_tag="ACG8NF_001892"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0472368.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8NF_001893"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8NF_001893"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0472369.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8NF_001894"
     CDS             13425..14372
                     /locus_tag="ACG8NF_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0472370.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8NF_001895"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8NF_001895"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0472371.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8NF_001896"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8NF_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472372.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8NF_001897"
     tRNA            15637..15709
                     /locus_tag="ACG8NF_001897"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tta
//
LOCUS       DAZKOM010000064        15480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000064 DAZKOM010000000
VERSION     DAZKOM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.064"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG8NF_001898"
     CDS             complement(1016..1357)
                     /locus_tag="ACG8NF_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0472373.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG8NF_001899"
     CDS             complement(1357..1587)
                     /locus_tag="ACG8NF_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0472374.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACG8NF_001900"
     CDS             1874..2212
                     /locus_tag="ACG8NF_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472375.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACG8NF_001901"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACG8NF_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACG8NF_001902"
     CDS             complement(3711..4292)
                     /locus_tag="ACG8NF_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0472376.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACG8NF_001903"
     CDS             complement(4475..5101)
                     /locus_tag="ACG8NF_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0472377.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACG8NF_001904"
     CDS             complement(5123..6454)
                     /locus_tag="ACG8NF_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0472378.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACG8NF_001905"
     CDS             complement(6609..7010)
                     /locus_tag="ACG8NF_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0472379.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACG8NF_001906"
     CDS             complement(7096..7302)
                     /locus_tag="ACG8NF_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472380.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACG8NF_001907"
     CDS             complement(7397..8899)
                     /locus_tag="ACG8NF_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0472381.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8NF_001908"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8NF_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0472382.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACG8NF_001909"
     CDS             complement(9470..10087)
                     /locus_tag="ACG8NF_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0472383.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACG8NF_001910"
     CDS             10308..11681
                     /locus_tag="ACG8NF_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0472384.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACG8NF_001911"
     CDS             11756..12868
                     /locus_tag="ACG8NF_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0472385.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACG8NF_001912"
     CDS             complement(12940..14577)
                     /locus_tag="ACG8NF_001912"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0472386.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACG8NF_001913"
     CDS             complement(14745..15353)
                     /locus_tag="ACG8NF_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0472387.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4295 a         3170 c         2696 g         5319 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tcataggtaa aagaaacatg acatttaatg acaaaataac gaattattta atatttgtaa
//
LOCUS       DAZKOM010000065        15401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000065 DAZKOM010000000
VERSION     DAZKOM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.065"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1165)
                     /locus_tag="ACG8NF_001914"
     CDS             complement(80..1165)
                     /locus_tag="ACG8NF_001914"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0472388.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(1180..1899)
                     /locus_tag="ACG8NF_001915"
     CDS             complement(1180..1899)
                     /locus_tag="ACG8NF_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0472389.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(1896..3206)
                     /locus_tag="ACG8NF_001916"
     CDS             complement(1896..3206)
                     /locus_tag="ACG8NF_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0472390.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(3233..3823)
                     /locus_tag="ACG8NF_001917"
     CDS             complement(3233..3823)
                     /locus_tag="ACG8NF_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0472391.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(4141..5202)
                     /locus_tag="ACG8NF_001918"
     CDS             complement(4141..5202)
                     /locus_tag="ACG8NF_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0472392.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(5770..6837)
                     /locus_tag="ACG8NF_001919"
     CDS             complement(5770..6837)
                     /locus_tag="ACG8NF_001919"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0472393.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(6857..7336)
                     /gene="pssE"
                     /locus_tag="ACG8NF_001920"
     CDS             complement(6857..7336)
                     /gene="pssE"
                     /locus_tag="ACG8NF_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0472394.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(7353..7814)
                     /gene="pssD"
                     /locus_tag="ACG8NF_001921"
     CDS             complement(7353..7814)
                     /gene="pssD"
                     /locus_tag="ACG8NF_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0472395.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(7828..9078)
                     /locus_tag="ACG8NF_001922"
     CDS             complement(7828..9078)
                     /locus_tag="ACG8NF_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0472396.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(9456..10907)
                     /locus_tag="ACG8NF_001923"
     CDS             complement(9456..10907)
                     /locus_tag="ACG8NF_001923"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0472397.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(11228..11992)
                     /locus_tag="ACG8NF_001924"
     CDS             complement(11228..11992)
                     /locus_tag="ACG8NF_001924"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0472398.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(12020..12718)
                     /locus_tag="ACG8NF_001925"
     CDS             complement(12020..12718)
                     /locus_tag="ACG8NF_001925"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0472399.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(12730..13509)
                     /locus_tag="ACG8NF_001926"
     CDS             complement(12730..13509)
                     /locus_tag="ACG8NF_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0472400.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(13525..14469)
                     /locus_tag="ACG8NF_001927"
     CDS             complement(13525..14469)
                     /locus_tag="ACG8NF_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0472401.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(14515..15294)
                     /locus_tag="ACG8NF_001928"
     CDS             complement(14515..15294)
                     /locus_tag="ACG8NF_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472402.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         4723 a         3117 c         2100 g         5461 t
ORIGIN      
        1 aaaatcttga aatgtaatag gtttcttcta gctctttgat taattacgct aattttcatg
       61 tttggacaac cacaaaaaac tatacatttt caatcaaagc aacccattgt tttgtaattt
      121 ctttcaaatt aaatttttgt acattattat atgcgttcag tgacattctc attgctaaat
      181 cagtattctt agtaaacatt atcatgctct tagctaattg gtctatattc ttatcttcaa
      241 ccaacatccc attaaaatta tgatgtacca aatctctcgg accagtttta caatcataag
      301 aaataactgg taatccataa gactgtgctt ctaatataac cataggaaat ccttcataaa
      361 cagaactaag cacataacaa gatgcagatt tataatattc ctccacatgg tctgaatgcc
      421 ctgctatagt tactctttgc agatttaaat cttttatctg ctggcgaaga tctttttcta
      481 attcaccttt tcctacaatg ataagatccc aatcatctac ttttttttct acaattttcc
      541 aagcacttat catgtcagca aaatttttct cttccactaa tcgccctact gcaagaaatc
      601 tctttttatc ataatgatat tcgtactcga tattagatat acacggattt ggcatagtta
      661 taacaaaatc ttctgataaa ctatattcat caatatgttt ttgacgatct tcttctgtga
      721 gaaccacaag cctctttgca tattttttta tcaatttttt agccttgaat ctcttctttt
      781 ctttcagcat ttccattgtc cagtaattaa aatgttccca agaaatcaat ttgcactttg
      841 ttcctttaat tgcccttgta gtatacaaag acaacaaagt atcaatatca attactatat
      901 caaattcatt tttgcatata tacttgtgca atttccaaac cggatataaa taagttctat
      961 ataattttcc ctccgtttta ggatttaaca tataatcaac cttaatcgtc ttatctaacc
     1021 caaaataagg ctctccacta ttccaaaagc ttaaaatagt gacatcataa cctaaatcag
     1081 acaagctatt ggcaataata ctccctactc tttcagttcc cccagaacga gaaattacac
     1141 cactaatgaa acaaattttc ttcaaagtac ttttctcctt tataagtgta aaaatttttt
     1201 cagtgcaagt aacctgttaa attttttttg tccaattgtt gattttaatc tataaacaaa
     1261 tttttgttta ggtgttttcc aagatgcatt atattgatga atgcttattg tattactagt
     1321 tttataaatt tttccagtat cataatcctt ggcagcaaaa taatccgtag gatagatatg
     1381 caggccattt attatttgtt cgctgttatc gcgaaccaat ccaatagatt caagataatc
     1441 tgtaatataa tacggactcg gtttcaagtt caatgtacca tcattttgat aaaaacttaa
     1501 tgaatcgtaa atatccctta aacctttaat aacagaatgt ccttcttctg caccaaaacc
     1561 aataccgcca ttaacccaga tttccccttc aaatcccata taggcatgat tttcacgaag
     1621 tatatctaac gacttaatta gctcaacatc tgtatctaaa taaaatccac catgattata
     1681 aataatatca aatcttgcat aatctggaac aaaaccccat ttacgttctt tatatgcatc
     1741 ggccatataa cggttcttat ttaaatcata atttgattca ttccattcaa taatctcata
     1801 atcagggcaa tactttttcc agctttctat gcactttcta tcttttttcg gaagtggatt
     1861 tcccccgaac caacagtaat gtatcttctt aggaatcata atatctctcc ttttgtattt
     1921 ttttcactat aataatgctc tttccttttc acaacttcag gcatggctaa aatcatataa
     1981 atcagtggcc ccgttgtttc acccctaagt gttgtgttat acacaaaaaa gaataccgac
     2041 ataattgcaa taactttaac aagacttgca tctttatcct tcataaaaga tctttttgca
     2101 ttgaaaaatg tctttattat aaaaaatagg aatatcgaaa ctcctaatat tccaacttct
     2161 ataaataacc aaatatgatg aaaatactga taatgaagat taccaaacga tttataaaac
     2221 tcactgacaa aaaaattggt aaactctgca ttgccaatgc ctactcccat taggactaat
     2281 ctaaaattat tattaaatat ttcatctact actctagaga taccagttat tcgatttaaa
     2341 tcaccaacac catcatatcc agaatctctt gacgcatatt ctaaaagacc ttgaattgtg
     2401 aaattatcag catagctttg tccattatcg ttcaatgtaa taaatattgt tataaaaaag
     2461 gataatacta tcgacacaag tattacgcat gataatatct tgatcactac tttaaaatca
     2521 acttttaaag tgaatactat tgcaacaaat attataaata attcaggaat aaaaactttc
     2581 aattcagcta atgcagacat aaaaagtgct gcacaaacac ataaaataaa atggatgaaa
     2641 tttttttctc ccttgaaata tccgattgta acaacagtaa ctgtcacaca caaaattaaa
     2701 ttcaatggca tattacaacc tgaataattc caaaaaactc cacctatcat atctcctaca
     2761 attgttccag ttggataggt gaccatgaat ctttcaatta aacaaagtgg cagtgaaatc
     2821 cagaataatt tttcaattat agcgaatact ttatagtaat ctttaattgt taaatatgtt
     2881 gcacaattta tatatactat gaacatacga gtgttatttc gtatccccca gataatcaat
     2941 cctatatttt ctaaattaat aactgagcta caaatagaaa ctagtaaaaa caaaactatc
     3001 agcatctcat ttttgctagc atatattcct tttctgcgtc taaagctaag tgcatttaat
     3061 aacacaaaga tattaattat atctcccaaa tataaaattg ctcttggggc gttaaaagta
     3121 tatacaagaa ctatggagat tgcaaaaaat gccaacgtga acaatgtcca atctgctcct
     3181 gaccatcttt ttattgaaat acgcatcgtt attcaccttc tttcgctaaa aattattcta
     3241 aaaatacttt tgttaaatta acatacccta ttatttttgc cggtacacct gcaagaattg
     3301 caccattctc tagacaatct ttgataacaa tagctcctgc cgcaacaact acattgttag
     3361 ctatttttac attaccaata atctttgcac ccacgcatac acgtacatta tttccaatta
     3421 caggacactt aggatcgatt ccatcatttc caatacagtt attcccatat aagtaacaat
     3481 tttttcctat tatggcgtta ccattaataa caattccttg tgtatggtaa aatcttacac
     3541 cttcatcaaa tacattttca ccacactcta taccacattt tcttcccaaa ctattcattc
     3601 ttctgcaaga cacaatatac ataatcgcat aaatgatatt tttctttctc tttgaataat
     3661 aataagatgt aattctcatt cgacgagtat accgccaagc gttgtagtcc ggatgtgatt
     3721 tcattatttt tagtaatttt gtgtgttgtc ctttagattc tatatataga ttcttttcca
     3781 tacgaagaac cgcttttaaa tattctttac tgctaatttc cattcatatc tcctattaat
     3841 tcaatttata cttaacaatt ttattcggat ttcttgcaac aagagcatca ttcaatgcta
     3901 ctgaatctac aacgatggat atgttatctt catcgttgat tccaccggat acaatattac
     3961 ttataaaaat cgtaacattt tttccaaaag aagggatgtc cgtgatgtat aatgcccatt
     4021 ttccgatatt tccagttatt taaacctaac gaatttttac aatagtacaa gtatcacaat
     4081 cattaatata taactggaat ataaatctcc aaaaattcac tatagtacag ttttttatga
     4141 ttaaaatctc ctatttctac ccattccgct taaatatgct aatgttttaa ccccaataat
     4201 ggattggttt agtaaaaaat agttaaatat tcgttgttta attggatttt gtttatatgg
     4261 gtatgagtga atacaggcaa tcaactcatc gttcataagt atcttagaca aataggagtt
     4321 taaatcaaat tctttattaa aactagcata tctaactgcc ttttgaatac aagtacgaca
     4381 gcgtcccaac aaaagtctcc ttgttttgtt tacaaatatc tcattttcta atatatagct
     4441 taaatcgcct tcaatttttt taactaattc aatattacaa tcgaactcat tatctctaaa
     4501 attatgtgtc aatgaattat tattattaca tctataataa tagatacaat ctttaataaa
     4561 acttgttatt tttgaatgtt gcaaatatga aatatggaat atgatatctt cagatatata
     4621 atccctttct gacggaaact tgatattgtg ctgttgaatt atctcagatg aatacagcgc
     4681 atgccaaact gacattggca atataaaatc gactttataa ctcggctcag tgccaatcat
     4741 tccaagcaac actttttcaa ttatagcgtt atttgtaaat atcgtactat catatcttgc
     4801 tggctttgaa ataatattat tatcatcata atattcataa tatccagcat aacatgtgtc
     4861 tgcattactc ctttttatac catttaacat tgcttcaata taattagctg caacaaaatc
     4921 atcagaatca ataaaagcaa catattttcc ttttacatgt tcaagtcctg tatttcttgc
     4981 caatcccaag ccagcattat ttttatgaat aatacgaatc cttgaatcct ttaatgcata
     5041 gtcttcacat attttattag atgaatcctt agatccatca tccacaatta taatatctag
     5101 gttcttatat gtttgattac acactgattc caaacaactt cctacatact tttcaacatt
     5161 atatacaggt atgattacac taattagatc actcgaattc atccttctat cctttcactc
     5221 acgttttctc aattagtcaa tgacggcatc aacgattttc ttaaattctc tgaaaaaaat
     5281 agactctgat cttgatgatg tgaatgacat actatcttct tctagaaaaa ttatttcctt
     5341 taaattattt attaaatcaa taatatgatc ctcgtaagta tgctttccct tggaataatg
     5401 aaaatatact tttgtattac acaatattgc aattttattt cttaatatga ttatttcact
     5461 cattcattac tgccacatat acacaactta taaccacccc ctgcaaagtt tagggtaagt
     5521 tatcagataa tatcttcctc ttcatcatcc agaatacaaa atctatttat ttatagaaat
     5581 atcattaatg tttttatata atgtaccgtg cttgtactct tgctctatta caagcactaa
     5641 aacactacta ttcatttgaa ttcttacacg gataaaaatt tacatcgtct atttcaatat
     5701 tttttctctc tattttattc acttaataag ttcattacat aatcgaaaca ttttttattt
     5761 tccttgtcat cactccttgc tccctttttg atacagattt aaaattctat caaagaaatt
     5821 tccatcgtta aatttcgtta ttgccaaatc cttcgaacaa cgtgaaaatt ttacgtattt
     5881 atctttatca ttgattttta gtattgcaga tgtcaacgct gatacgtcac caggttgaaa
     5941 caaatatcca ttttctccat cacgtactgc tgaagaaata tctcctacat ctgtagcaat
     6001 tacaggcatt ccataagaaa tcgcctctag aatagatata ggcaatccct cgttatagga
     6061 cggtaaaatc atcacatttg atcttctata gcactcctcc tttttttctc ccacaaccca
     6121 acctgtaaat tcaaaatacg attgcaaatt atattcttct atcaattctt tgagcgatat
     6181 ttcttcatca cctgctccag caatctcaaa aataaacttt ttttctggat attgttctgc
     6241 aatgttttta actgcttcta ccaaatcgtg tacacctttt cgctttatca aaactcccat
     6301 aaacagaaat cggcacattt ttgaattcca ctttatggta tattctggaa ctacaataga
     6361 attttctaca acaacagttt tacaatttgg ctcaatttct ttaattatct tcttccattt
     6421 ttctccaagt acaatcgtgg aatcagactc tcttaacagc aagcgaatct gattttgttt
     6481 ttttacagaa acactatcat accatttttt aaattccgat ccatgaagat ggatgattac
     6541 aggaatattt aataatctaa accacttatt cagcacatat ttcctactaa aactccctct
     6601 gtacgacatg tgcatatgaa taatattagg tttactcaat aaagagatac ctataatttt
     6661 agcaaatgca tacataaaat ataaagattt aaatattgca tttccaccct tataactcgg
     6721 tataaaagtc atctcgatat tctttttttc ccaattaaaa ttgagcattt gtgtaattat
     6781 acttgtaatt ccacctttta ccgagctgtc attaccaatc ataagtactc tttgcataac
     6841 gctttactcc ttatatttat attttttcta ttaatacctc tatcgaatta ataatcgttt
     6901 ccgtgttaga tacatacttg ttaaacatcc gaactcttgt ttctttaacg gcatcttcta
     6961 aattttctaa ttcataacaa ggatagataa tattcatctc atcaaattgt ctaattaact
     7021 gcagttggtg atcatcgaca tgctcaccat acttagccaa tcgaggaaca gcaatgaccc
     7081 ttttaccttt ttttacggcc ccaattatag cacctgtacc tccatgagta atcaccattt
     7141 ctgctttttg catagtttca gtaaactcat ccctatttag aaatgcttta taggaataat
     7201 ttagtggttt ataatcacta taaccactct gtgcaaatgt actttcgatt attactcctt
     7261 tttcaattaa atcatcaata gcttttaaca atctatcaaa ttgaaatttt tgtgaaccta
     7321 atgttataaa tatcattttt ttctcctatt atttaatata tcccacccaa ataaatagca
     7381 ttaggataaa attgtagcat agtttcccac tgaacataaa attgatctgc aaaatgatac
     7441 atcaattttc ctgtctgagt agggctggtt acttttgcaa acgattcaat ataaatcagt
     7501 ttttttccca tcatttttgt tattaagcag atagggatca tagctaaaac acctgtacaa
     7561 atcacgagat ccggtttttc ctttatatat ataataattg atttgaataa attaataagt
     7621 atagtacaca aaaaaaactt ttcttttcta tttacttgtc tcatgtagta cgttttttcg
     7681 ccttttgact cagctgaata ttctgtctgt tctgttataa caaaactctc atacttctcc
     7741 atcagtggtt ttaacatcat taactgttca taatgccccc cagaggaagc cgcaaaacat
     7801 atctttttgt tcatattaac ctctctatca gtcacgctga aaaatatctc tagtgtaaac
     7861 tttttcttta atatcatcca agcaggtatc atatcgattg gcaataatag aatcacacat
     7921 cgccttaaac tgctctaaat cgttgataac cttacttccg aaaaaagtct ctccatcttt
     7981 cagtgcaggc tcataaatca ccacagtcgc acctttagct tttacgcgct tcataactcc
     8041 ctggattgaa ctctgacgaa aattatcact gttgctcttc atggtcagcc ggtatatacc
     8101 gattaaaact ggattttccc tgttgacatc atactgatta tattcagcgt agttgtaata
     8161 gcccgccatc tgaagaacac gatctgcgat gaaatcctta cgggtacgat tagattcaac
     8221 gatagcctca atcaaatttc caggaacatc tgcatagttt gccaacaact gcttggtatc
     8281 ttttggcaag caataacctc catatccaaa ggatggattg ttgtagtgag ttccaatacg
     8341 aggatcgaga caaacaccat tgattatctg ctgagtattc agccccttca tttcagcata
     8401 agtgtctaat tcattgaaat agctgactcg cagtgctaaa taggtatttg caaacagctt
     8461 tactgcttcg gcttcagtga atcccataaa aagagtatcc acgttctcct taattgcact
     8521 tttctgcaga agtgttgcaa aaacatgagc agcatttaac aagcgctcat cagtcatatc
     8581 agtaccaaca ataatacgag atggatacag attatcatat aaagccttgc tctcgcgcag
     8641 aaactctgga ctaaaaataa tgttcttact ccccgttgtc tcacggactg attcagtgta
     8701 gccaacagga attgtagact tgatgaccat gatggtatca ggattcacgt ccatcacaac
     8761 ctcaataact gactcaacag cagaagtatc gaaatgctga gtacgactgt catagttagt
     8821 cggtgtagca ataacaacga aatctgcgga tttataagcg ctccttgcat ccaaagtagc
     8881 agttaagtca agttcttttt ctgtaagata cttttcaata tattcatcct gaattggaga
     8941 cttccgttta ttaatcagtt caatcttttc agggataatg tctaccgcgg ttacctggtg
     9001 atgctgcgcc aatagtgtgg cgatactcaa accaacataa ccggtgccgg ctactgcgat
     9061 gttgtacttt ttcttcacaa tatctcctct ttcctctctt aagtatggag aatgttactc
     9121 ctacggctat tcatcatgtc aaaataacac agcttagaat gttgataaaa taattttcca
     9181 gaatgtgata aaatctcttt catttataaa ccacacatct aattttatct attctccaca
     9241 agacatgtag agtattcttt aataacaaga tacgacctta ctaaatactt caaaaaatta
     9301 tcatatttaa ttcataaata taaatcttca ttttaaatgt atgcccaaaa atttatctgt
     9361 aatgcgtcat caaaatctag attccaaatc tttatcaatt tttatccact cattctacca
     9421 ctttagtaat attttctatt tataacactt tcgtcctaca ttgatccccc cctctgaagt
     9481 acagttttta ctgtcttaaa taaaatttta aaatctaacc ccatactcca attttttata
     9541 tattccgtgt caagttttgc tacttcttca aaatcagtaa tatcacttct accacttacc
     9601 tgccacattc cagtaattcc tggcttaata gcaattcttg cacgatgatg ataatcatat
     9661 tcttcatact ctgatttaag gcatggtcga aatcctataa ggctcatctt tcctgttaga
     9721 acgttgaaaa attgagggaa ttcatctaaa gacgtggatc taataaattg accgattcct
     9781 gtcttttttg ttccatctgg aagtatttta tttccgataa ttcttggatc aaactccatc
     9841 ttaaacataa gattattgct catcttattt tctttcatta attctacttt tctttcttct
     9901 gcatccatat acatacttct aaacttatac atcttgaacg gtttaccatt ctgacctata
     9961 cgttcttgtg caaagaaaat tgggcctggt gaattaatat aaataagtgg agcaataaag
    10021 ataaaaataa ttcccgttgc aatacatcca acaatacctg caagaatatc aattgttcgt
    10081 tttataaatg cctgttgagt tgtcataaca ttgatacttg ttgtaagtac tgtatattct
    10141 cctatacgac caatagattg ttttcccaaa gcactatctg atacttttgc aagattcaaa
    10201 tgtacagtaa ggcccatttc agagcatcgt tcaataaatt ctttcataca cgaagtactt
    10261 ttattcaaaa taataaagac ttcatctacc catccctgac ataagtaatc cgctgcattt
    10321 ttggaatcag aaacaactgt cactccctca acatcttcac caatcatgct tttatctatg
    10381 ataatgacac catttatttt atacatttta tagttatgct ctttcacatt acgaataact
    10441 acttcagcaa tactactaga tgtaactata ataagtgatt tgttcccctc atttttcatt
    10501 ttatgtttca agtaatgttt ccataagagg cgagttatat atgtaattac aacgtataaa
    10561 cctcccatca agaaaagaac gactctagaa taatcatttc caccctgaac actaatgaga
    10621 tacaaacttc ctaacagcac caacataatc gcttgcttta cagaagaagc aaattctata
    10681 tagtaccctc tttttaatac acctctatat gcttcaaaca tgaaaataac taaaagatct
    10741 gccagttcaa tgaagactgc catgttttga tacattggaa tcacataagg attccaatct
    10801 acctggcgta agacatacga tagataaaaa gcaagctgga tacacaataa atctaaaata
    10861 ataaaatcaa tatgtttaac ccatcccctt gaacttttct tgtacattcc tttttactcc
    10921 cctcttttta ataaaataaa ttgtattact tgttttctct cctaaatatc tttctgaagg
    10981 attattgact cgacaaattg gcttgtggat attcttatat atagaacatc cgtaagccaa
    11041 cttaaaactc cttaacaaaa tttttttact catattgtag atcaaaacaa gtatccaaga
    11101 aatcatattt tatttctttt ttctgcttac tcaacaacag ctaaaagaaa aatcatcata
    11161 aacaaattca actaagccaa cgatcccctt gtgaaatcaa gttttttctt gatttacgaa
    11221 taaagagcta aaataaacca aacttcttct tttttacctc ttcatagact ggataactga
    11281 tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac
    11341 caaagtcctt tgcaatctgc tcatagcact cttttaagta aaaggtgcgc ttagagaccc
    11401 catgggcatc agaagcagcc atctgcacga gattatgttt gaccatcgtt ttggctgttt
    11461 tctggatgtc tttgccaaat accccaacat aacttggcgc agtgagttgg gccaaacatc
    11521 ccatatctag gaacggaagc aaatgatttg ggtcttcgcg aaaatgcacg ttgcgttctg
    11581 gatggacgat tactggtgtc tttcctagtt ctaacaactc aaaaaaaagc cgttctgcat
    11641 ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct aaatctgtaa
    11701 ataatatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt acttcttgtc
    11761 cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac gaagcgacta
    11821 gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt ggtgtgcata
    11881 aaatatgcgt gatgccttgc tgaatcgctt tttcagccat tgcaatactt gcttctaagt
    11941 tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc atttctcttg
    12001 tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg ctatgttctg
    12061 ctccgttgta cacaacacct aagactcgtg cttgtaccat ctccagcagt tcttttgctt
    12121 tagtcaagga ttctttacgt gacatatttt cacgtacgac caataaagtg ccatccgttt
    12181 ttaaagacat gatctgtgca tctgttacgg ctactactgg tggcatatcg aatatcacca
    12241 cgtcaaataa ctgtcttgct tcggatagga tctgatccat tcttggcgag cttaacaatt
    12301 ctgatgggtt gggtggctta ggtccactag gcaaaatcga caagttctcg actggcgttc
    12361 gttggatcac atctgctaca cttcctgatg aactaagtgc tgtacttaat ccactggcat
    12421 tgttcagctg gaatgtctta tagacgacgg gtttacgtaa atctgcatcg actagtagaa
    12481 ctcgttgacc tgatttagca aaaacgactg caatgtttgc tgcagtggtg gatttccctt
    12541 ctccaggacc tgatgaagtc acgacaatcg ttttgatttg ctgaccagct gcggaggcaa
    12601 actggatatt cgtccggatc gtacgatatt gttcggcgat tggtgaagat gggtttgtta
    12661 atgtgatcaa actgactgca tgagtttgtg ctgctttttg tttctgtgtt cgtgccattt
    12721 ttcttcctcc tagactcttg agcggcttct gcgtgatggg gctgttttat cgtttgtagc
    12781 ttttggtcgt gcaggagctg ttcctttttt acggatacca gcgttcaatt ctttcgctga
    12841 catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat atttttcatc
    12901 tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta ccatcattcc
    12961 taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg tcgtattggc
    13021 aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg cgttctcttg
    13081 gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg tacttattgc
    13141 ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat ctttgatttg
    13201 ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt cattgagcac
    13261 taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc
    13321 attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta
    13381 ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga
    13441 gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt
    13501 ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc
    13561 ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat tatcttgcat
    13621 gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc gataatctaa
    13681 agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact ctagagcatt
    13741 caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg ctacgccttc
    13801 tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt cataacgcat
    13861 acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata
    13921 gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag
    13981 ttcctttagt cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt
    14041 ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc
    14101 ttttgtgtca cgacctacga tgtcgacata cgtatctcga ggaatgctga cgatcgttgt
    14161 ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac
    14221 ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt cttgtttgtc
    14281 cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc
    14341 aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa
    14401 tacgataaga actgctaaaa taatcaaaaa tatttttttc cctcttgatt ttcttcttcg
    14461 tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata
    14521 gcttatattg ctatagccca tttgctgagc aacagtttgt gcttttgttg cgtatgcggc
    14581 tcttattttt tcattccgga tctgctctat ttgactgagc gccgtcaaat attgatcgta
    14641 agtagcggta gctgttactt gaccgtcttt cagcatcgta tcgaatagct gatctagttg
    14701 cgtgacttgt gcaatttggg tatcagcgaa tcctagatat tgcataacga ctgttttcca
    14761 agcactatcc ttaaaaaagc tcatattttc acgtacatgg gcgacatctg cacttgccaa
    14821 cttttctttg atgatgacat catccacagc ttgctgccag tttgatacgt ttttagtaaa
    14881 taatttattg acagccgtct ggatcttcag ttttgattct gcatcctcta actgcgttaa
    14941 gactgctttc ttttcttctt ggatcgcagc agcttcttta ggtaattctg attccttgat
    15001 gtcataatca tctgccgaaa gtttgataga agatagttta tgtgacaaat ttgaaactgt
    15061 ttcttctgtt atttcaggat ttaggaaaat tttttctttt gtactgtaca gcttatctac
    15121 ttcttttgat aaagaggtca cggtgtcttt ttgattggtg atggcagatt ctgcgtaatt
    15181 gatccgttga tgatattgcc gctgattatt gagatggtat ccaaacaagc aaatcacaat
    15241 agcgcctgtg cctaccgcca cagtagccaa taacttgacg aacggctttt ccatagattc
    15301 actcctaaat attgatgata gataagtaaa gatgtgattc attcacatca gaatgagcgc
    15361 tttttatttt aaaagcataa ttaaactaat atattcatag a
//
LOCUS       DAZKOM010000066        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000066 DAZKOM010000000
VERSION     DAZKOM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.066"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8NF_001929"
     CDS             2..1021
                     /locus_tag="ACG8NF_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0472403.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8NF_001930"
     CDS             1018..1785
                     /locus_tag="ACG8NF_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0472404.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8NF_001931"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8NF_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0472405.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8NF_001932"
     CDS             3750..4070
                     /locus_tag="ACG8NF_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0472406.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8NF_001933"
     CDS             4070..4420
                     /locus_tag="ACG8NF_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0472407.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8NF_001934"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8NF_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0472408.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8NF_001935"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8NF_001935"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0472409.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8NF_001936"
     CDS             6414..7439
                     /locus_tag="ACG8NF_001936"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0472410.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8NF_001937"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8NF_001937"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0472411.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8NF_001938"
     CDS             8517..8957
                     /locus_tag="ACG8NF_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0472412.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8NF_001939"
     CDS             8960..9562
                     /locus_tag="ACG8NF_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0472413.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8NF_001940"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8NF_001940"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0472414.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8NF_001941"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8NF_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0472415.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8NF_001942"
     CDS             12294..12689
                     /locus_tag="ACG8NF_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472416.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8NF_001943"
     CDS             12689..12940
                     /locus_tag="ACG8NF_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472417.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8NF_001944"
     CDS             12983..13378
                     /locus_tag="ACG8NF_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472418.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8NF_001945"
     CDS             13375..14631
                     /locus_tag="ACG8NF_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0472419.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8NF_001946"
     CDS             14643..14966
                     /locus_tag="ACG8NF_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472420.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8NF_001947"
     CDS             14959..15225
                     /locus_tag="ACG8NF_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472421.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKOM010000067        14815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000067 DAZKOM010000000
VERSION     DAZKOM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.067"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8NF_001948"
     CDS             complement(296..1024)
                     /locus_tag="ACG8NF_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472422.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8NF_001949"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8NF_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0472423.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8NF_001950"
     CDS             2805..3743
                     /locus_tag="ACG8NF_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0472424.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8NF_001951"
     CDS             3755..4666
                     /locus_tag="ACG8NF_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472425.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8NF_001952"
     CDS             4690..6162
                     /locus_tag="ACG8NF_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0472426.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8NF_001953"
     CDS             6213..6824
                     /locus_tag="ACG8NF_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0472427.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8NF_001954"
     CDS             6854..8767
                     /locus_tag="ACG8NF_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFQ0472428.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8NF_001955"
     CDS             8895..11876
                     /locus_tag="ACG8NF_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFQ0472429.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8NF_001956"
     CDS             11873..13534
                     /locus_tag="ACG8NF_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFQ0472430.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8NF_001957"
     CDS             13574..14506
                     /locus_tag="ACG8NF_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0472431.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZKOM010000068        14625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000068 DAZKOM010000000
VERSION     DAZKOM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.068"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8NF_001958"
     CDS             4..816
                     /locus_tag="ACG8NF_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0472432.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8NF_001959"
     CDS             813..1613
                     /locus_tag="ACG8NF_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0472433.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8NF_001960"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8NF_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0472434.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8NF_001961"
     CDS             3110..4819
                     /locus_tag="ACG8NF_001961"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0472435.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8NF_001962"
     CDS             5031..9389
                     /locus_tag="ACG8NF_001962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0472436.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8NF_001963"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8NF_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0472437.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8NF_001964"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8NF_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0472438.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8NF_001965"
     CDS             11227..11520
                     /locus_tag="ACG8NF_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0472439.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8NF_001966"
     CDS             11517..11828
                     /locus_tag="ACG8NF_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0472440.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8NF_001967"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8NF_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0472441.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLQEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8NF_001968"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8NF_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0472442.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2599 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgcaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAZKOM010000069        14257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000069 DAZKOM010000000
VERSION     DAZKOM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.069"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8NF_001969"
     CDS             476..1339
                     /locus_tag="ACG8NF_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0472443.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8NF_001970"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8NF_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0472444.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8NF_001971"
     CDS             3339..3860
                     /locus_tag="ACG8NF_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0472445.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8NF_001972"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8NF_001972"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0472446.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8NF_001973"
     CDS             5941..6759
                     /locus_tag="ACG8NF_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0472447.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8NF_001974"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8NF_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472448.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8NF_001975"
     CDS             7228..7806
                     /locus_tag="ACG8NF_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0472449.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8NF_001976"
     CDS             7901..8038
                     /locus_tag="ACG8NF_001976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472450.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8NF_001977"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8NF_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0472451.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8NF_001978"
     CDS             9713..10738
                     /locus_tag="ACG8NF_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0472452.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACG8NF_001979"
     CDS             11061..11939
                     /locus_tag="ACG8NF_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0472453.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="ACG8NF_001980"
     CDS             12415..12531
                     /locus_tag="ACG8NF_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0472454.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACG8NF_001981"
     CDS             13459..13797
                     /locus_tag="ACG8NF_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472455.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACG8NF_001982"
     CDS             13891..14121
                     /locus_tag="ACG8NF_001982"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0472456.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4525 a         2378 c         2725 g         4629 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagta
//
LOCUS       DAZKOM010000070        13977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000070 DAZKOM010000000
VERSION     DAZKOM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.070"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG8NF_001983"
     CDS             140..460
                     /locus_tag="ACG8NF_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472457.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8NF_001984"
     CDS             912..1112
                     /locus_tag="ACG8NF_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0472458.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG8NF_001985"
     CDS             1259..4048
                     /locus_tag="ACG8NF_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0472459.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG8NF_001986"
     CDS             4095..4622
                     /locus_tag="ACG8NF_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0472460.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG8NF_001987"
     CDS             4643..5833
                     /locus_tag="ACG8NF_001987"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0472461.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8NF_001988"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8NF_001988"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0472462.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG8NF_001989"
     CDS             7966..8640
                     /locus_tag="ACG8NF_001989"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0472463.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG8NF_001990"
     CDS             8637..8978
                     /locus_tag="ACG8NF_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472464.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG8NF_001991"
     CDS             9008..9367
                     /locus_tag="ACG8NF_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0472465.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG8NF_001992"
     CDS             9627..9749
                     /locus_tag="ACG8NF_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472466.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG8NF_001993"
     CDS             9891..11975
                     /locus_tag="ACG8NF_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0472467.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACG8NF_001994"
     CDS             12286..13752
                     /locus_tag="ACG8NF_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0472468.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3938 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgtttt
//
LOCUS       DAZKOM010000071        13959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000071 DAZKOM010000000
VERSION     DAZKOM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.071"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8NF_001995"
     CDS             <1..135
                     /locus_tag="ACG8NF_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0472469.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8NF_001996"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8NF_001996"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0472470.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG8NF_001997"
     CDS             complement(2412..2663)
                     /locus_tag="ACG8NF_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0472471.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8NF_001998"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8NF_001998"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0472472.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG8NF_001999"
     CDS             3613..3831
                     /locus_tag="ACG8NF_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472473.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="ACG8NF_002000"
     CDS             complement(4120..4632)
                     /locus_tag="ACG8NF_002000"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0472474.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG8NF_002001"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG8NF_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0472475.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="ACG8NF_002002"
     CDS             complement(7085..7528)
                     /locus_tag="ACG8NF_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0472476.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="ACG8NF_002003"
     CDS             complement(7529..8317)
                     /locus_tag="ACG8NF_002003"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0472477.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG8NF_002004"
     CDS             complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG8NF_002004"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0472478.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="ACG8NF_002005"
     CDS             complement(9432..10019)
                     /locus_tag="ACG8NF_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0472479.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG8NF_002006"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG8NF_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0472480.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="ACG8NF_002007"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="ACG8NF_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0472481.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="ACG8NF_002008"
     CDS             complement(12936..13871)
                     /locus_tag="ACG8NF_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0472482.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZKOM010000072        13945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000072 DAZKOM010000000
VERSION     DAZKOM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.072"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACG8NF_002009"
     CDS             complement(<1..220)
                     /locus_tag="ACG8NF_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0472483.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKF"
     gene            complement(699..1418)
                     /locus_tag="ACG8NF_002010"
     CDS             complement(699..1418)
                     /locus_tag="ACG8NF_002010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0472484.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1467..3113)
                     /locus_tag="ACG8NF_002011"
     CDS             complement(1467..3113)
                     /locus_tag="ACG8NF_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0472485.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3292..3882
                     /locus_tag="ACG8NF_002012"
     CDS             3292..3882
                     /locus_tag="ACG8NF_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0472486.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3974..5479
                     /locus_tag="ACG8NF_002013"
     CDS             3974..5479
                     /locus_tag="ACG8NF_002013"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0472487.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5563..6915)
                     /gene="celB"
                     /locus_tag="ACG8NF_002014"
     CDS             complement(5563..6915)
                     /gene="celB"
                     /locus_tag="ACG8NF_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0472488.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6915..7433)
                     /locus_tag="ACG8NF_002015"
     CDS             complement(6915..7433)
                     /locus_tag="ACG8NF_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472489.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7449..7775)
                     /locus_tag="ACG8NF_002016"
     CDS             complement(7449..7775)
                     /locus_tag="ACG8NF_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0472490.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7788..9767)
                     /locus_tag="ACG8NF_002017"
     CDS             complement(7788..9767)
                     /locus_tag="ACG8NF_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0472491.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9788..10102)
                     /locus_tag="ACG8NF_002018"
     CDS             complement(9788..10102)
                     /locus_tag="ACG8NF_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0472492.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10270..10563
                     /locus_tag="ACG8NF_002019"
     CDS             10270..10563
                     /locus_tag="ACG8NF_002019"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0472493.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10641..11753)
                     /locus_tag="ACG8NF_002020"
     CDS             complement(10641..11753)
                     /locus_tag="ACG8NF_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0472494.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11906..12754
                     /locus_tag="ACG8NF_002021"
                     /pseudo
     CDS             11906..12754
                     /locus_tag="ACG8NF_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13107..>13945)
                     /locus_tag="ACG8NF_002022"
     CDS             complement(13107..>13945)
                     /locus_tag="ACG8NF_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0472495.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4351 a         2852 c         2386 g         4356 t
ORIGIN      
        1 aaaatttgaa attttcccaa cccacccaag gactttccct caagctagcc aagaacccat
       61 ctgctgaaat atgaaaattt ttgaatgcta cgacattcgc cattacaggt atgtaaaaga
      121 aaaaaatcat ccaaagcata cccggtacag ccatcaggac aagtgcttta taacgccata
      181 gattcataaa aaaatgattg aacccctttc gttttttcat ctaaaaaccc ctttcgattc
      241 attcgcaacg taattatagc gctttcaata atgagaataa ataaacaaag tatagggaaa
      301 atgaaacaaa gtataggaga ggttgtgaaa tcagcgtttt ggcctgagta ttagagaaaa
      361 aaacgggaaa atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg
      421 attgaacacc gtttatcaga ggttgtgaat tcagcgtttt ggcctgagta ttagggagat
      481 cgtgtcctta ctatctaact ctgagtcaca aggaacaatg gggactgtaa caaaagtctt
      541 gtcacagtcc cttattccga acaaacggtg ttccagaagt agcttctacg gaaataagcc
      601 gaaatttcac aaaaaagcca tccgaaagtc agacggacaa gtctgctttt cagatggcgc
      661 tacaactact ctgcttattc ttttagctat ccattcaatt atttttctct cacttgttct
      721 agctcttctt tggtcatggg tcgatattgc cctttattca aatctttagg cagccaaaat
      781 tttcccatac ggattctttt taaatataag acttcttttc ctactgcttt gaccattcgc
      841 ttaacttggt gaaactttcc ttcatgcaaa atcagacgga taattgaagt ttctgtctct
      901 tgatctactt gatcaatttg gagttttgcc ggcaatgtct gctcatcgcc atcaataatc
      961 agtccttctg caaataaacg gatatcttct tctgtcatta taccgctaac ctcagctaga
     1021 tattctttct ccacatgttt tttaggtgat agtaactgat gggcaaacac cccgtcattc
     1081 gttaaaatca agaggccctc tgtgtcttta tctaatcggc cgactggaaa aagatcttcc
     1141 cgataatcct ggtcttccaa taaatcgatt acggtttgat catgattatc ctgagtagca
     1201 gaaatgactc ctgcaggttt atgcaaaatg tagtaaaaat ctttttgata cgtaatgatt
     1261 tctcctaaat aagtaaccgc atcttcgaat tcatttactt gcgctttatc gcttttcacc
     1321 acttgtccat tgacttcgat cgttcctttt ttgattaatc ctttgacttc tttgcggctg
     1381 ccaagtccca tatctgctaa aaacttatct aaacgcatgc gtttccccca tttcaaaaga
     1441 gcatcaatct agctcttctg attcacttat tttattttta atttatgtcg taaacgcgct
     1501 attcctggtc ctagtagtct ttctgccaag cggattttca aagctaaata accatacact
     1561 aaagccccta tacctgctac tacaagaatc aacaatagag actggaattt gctatcttcg
     1621 tttaaaaaag caccaaaaat catctttgtg atccacgaag ccaaaagcat aatcaaggta
     1681 agaataaaga tcagcaatgt cctgcgccag acaaaacttg gtttaaagcg ggctacttca
     1741 tggattctct ttaagatcaa ggtacaagac acagcaaatc cgatcattgt cgccaataaa
     1801 gggccgtata cttcaaataa acgaatggct gggtattgaa gaaggagttt cactagaaac
     1861 ccaataacca aataattaat tgccgccttg ttttcaaaca ttccttgcag catgttggaa
     1921 actagcatat atagtcctag gaagagtcct acaaaacttg cttggatcag tacattactt
     1981 cctagagaat ccggtgtata aaataatgta tttagcggat aggcaagtaa cataacacca
     2041 aatgtcgccg gaaacataaa aaatgaaaac aactgtaaat ttccgcttgt caattttgcc
     2101 agacctactc gatctttcaa cgtaacagct tctgtaatca acggtaaccc agtagacgcg
     2161 atcgatgttg ccaatgcaat aactaccatc gtcaacttat ctggattggc actaaaaatc
     2221 gaaaaaagat ccagtaactg tgcattggaa tactcggtgg atgcagacat gatcttcata
     2281 aaagtgaact gatcgactaa tttgaagatc gttacccctg aacccacaat aataaacgga
     2341 atcgcttctt taacggtatc taacaacaat tccttcgttg caatatttat tttgttttcg
     2401 ctgtagtgaa taaacgccga tgtatacgct tgatgtttct tcaaaaaata caaaaggaca
     2461 agaatacttg ctagcatacc gataaatgcc gcgaaagttg attgcgtgac cgccgtcaca
     2521 tagtcaccat ccatgacttt catgatgatg aatgtagcca atagcatata aaaaatacgt
     2581 gccacctgtt ctacaatctg cgacagcgca taaggcatca tttcttgatt gccttgcaaa
     2641 taccctcgta tcacactcat acaaggaaaa accaatacag cgatactcaa agaacgcatc
     2701 gttggaatca gttcttctcc gccaccagaa gcatgagcta gccaaggaga ggcgatgtac
     2761 atgaacaaag caaacaacag gcctaacccg cccatcatct gtaatgcacg gataaataat
     2821 cgtcgtgaag tgccatattc attcaatgaa ttatagcgtg cagtttgttt tgcaatcgca
     2881 gcaggtattc ctgctgtaga gatcagtaga aacaatgcat atatattgta tcccatattg
     2941 aataatccat tggcagcctt agcattttcg cccatccaag cataccaggg aatgatatag
     3001 acagcgccta gcaatcttga aatgatatta ctggcggtca tccaagcaga gcctcgtgcc
     3061 attttttctt gcggtgttaa ttgatctaat gctggttttc tttgattatc catcggtcag
     3121 attccaactt tctacaaaac attactatca ttttaattgg taaaacagaa actggcaatc
     3181 gattttatat aacttttata agaacaattg tttttttgaa ttcggtttcg tttttttggt
     3241 aaagtaaaga aatatacagc cttttatggc taacatttat aggagatatc aatgacacaa
     3301 gaaataaaaa aactacccgt tatattttta ggtcttttct ttatcacagt tggattgaat
     3361 tggttcttgc tccctcacga tatagcttcc gccggagtgg gcgccatcgg acatttagtt
     3421 gaaacggcct tttctatcca acgctcagtt actgtttgga gcatcaatct tatgatgctg
     3481 cttctagccg ctgtcctatt aggaaaatca gtatttttta agactgtagc gggaagtctg
     3541 ctttttcctg ttgttttaga gattgttcca aaagtggaaa tgatctcctc acatttcttt
     3601 tcactgattt caggaagtct tttgttcagt cttggggttt acacattgta cacagtcggg
     3661 gcctcaaatg gcggtataac gataccgccg attattttcc acaaattttt tcggcttcca
     3721 atgccgcaag gacttttact taccaatagt cttatcgtct ttttgaatta ttatgttttt
     3781 ggattgttgg agacactatt cgttctccta tctattacag tcagttcatt gtttatgaaa
     3841 atactcatcc gcttgtcccc tgtgtcaaga aaatttgaat aaagctggat tttgtcatag
     3901 cggaaacaaa cgatatatta aaaagagtgt gctataataa accaagaaat tgaataaagc
     3961 gagggatcaa tcaatgagct tctctttaga agacatccgt catttactat taaaagaaaa
     4021 tctattaaaa gaatttgttt caacaagagg ctggcatctt gatgtaccag aaaaagctga
     4081 atttcttcag ttaagttatg attcaagaaa agctgatgct tctactttgt ttttctgtaa
     4141 aggaatgaat ttcaaagaag aatatttgga ttcagcaatc gaacagggta ttcaatatta
     4201 tgtatcagaa caaccatacg aaaattcagc agtaggaatc atcgtaacgg atatccgtaa
     4261 agcaatggcg cttcttgcta tggcttttta cgactatccg caaaataaat tgaaagtgat
     4321 cggattcact ggaacaaaag gtaaaacaac tgctgcgtac tttacaaaag caatcttgga
     4381 tcacacaaca aataaaaaaa cagcattgct ttctactatg aatacaacgt tagatggcaa
     4441 aacatatttc aaatcccatt tgacaacgcc tgagtcatta gatttatatc gtatgatgtc
     4501 agaagcagtt gaaaatggta tgactcatct agtgatggaa gtttcttcac aagcttacaa
     4561 aacacaacgc gtatacggct tgactttaga tgtcggaatc ttcttgaata tctcgcctga
     4621 ccatatcagt ccgatcgaac atccgacatt tgatgactat ttctattgca agcgacaatt
     4681 gatcttgaat tcaaaaacag ttgttttaaa tcatgaaagt gactattttg atttacttag
     4741 ggaaacagcc gatctattcc agattccaac gatcacttat ggtcgttcgg aagaaagcaa
     4801 ttatcaagtg atccgttcag ataaaggtac tcacggtttt actttatctt ctcatgaaga
     4861 tcgcttagct atctgcgata ctgcttatga tattttgctg gctggaggat tcaaccaaga
     4921 aaatgcagcc agtgcgatca ttgccgcagc actaacaggt gcctccagtc aagatgcaca
     4981 agacggatta aaagaagctc gtgtaccagg ccgaatggat caattgatcc aatccaatgg
     5041 agcgcatgtc tatgtggact atgcacataa ttatctaagc ttgaaaacat tgctgaaatt
     5101 tgccaaaaat gaacatccag atggccgtgt aattgttgta ttaggcagcc caggaaacaa
     5161 agcaatctcc cgtcgacatg attttggaaa agtgctatca gaaacagctg acatcgcctt
     5221 tttgacggca gatgacccag catttgaaga tccgaaaaaa atcgcggaag aaatcaatga
     5281 agcaattact aatccagatt tgatcgttca ttatgaaatg gatcgtcctg aagcgattcg
     5341 acgcgcttta gcagaaagcg ctccgcaaga ttcagtcgtt attgcgggaa aaggcgtgga
     5401 tccctatcaa aaaatcaatg gtgtagatga accttatgaa ggggattatg caatcgtgaa
     5461 gcggttgatt gaagaataat ggtatttata tactagtggt ttcccatttc taagaaaaaa
     5521 agggactgtg attttttgtc acagttcctt ttttcttaga aatcatttta acttttcact
     5581 agctaattct gctgcctttt ctctttcttc atttaataat ttattatcat agaatttgat
     5641 aaatgggaac cagataatga atgcgatgat tgcacaaatg actgctaaca cagcagcttt
     5701 ccaatcacca ccactaccaa caaatgctcc gatcccgact ggtgaaggcc acggcatctg
     5761 tgcaagcatc ggtttgacga tttccagccg aatggcaaaa taagcgagtg tagcagaagc
     5821 catcggagct aagaaaaacg gaaccgctaa atagggattg tagacgattg gcatcccgaa
     5881 aatgattggt tcattgatat tgaataatcc tggtacaagt gaggcttttc ctaacacttt
     5941 cagctgatca gatttcgcga ggaatgcaat aaagatgatc aatcctaaag tcgcacctga
     6001 tccaccaacg gttacataag aattgttgaa ttctcctgcc aacggataat ttgcgccttg
     6061 tgcgttagct gccatattag ctaatacgat cggggtcaag aaagaagtga tgatattcgc
     6121 tccatggatc cctacgatcc acaatgcatg gatcaagaaa taaatcacca tgacaccaag
     6181 ccatgtattc gtcaactgcg taacaaaccc aaatgggata gcaatcattt tgaagatatc
     6241 cgttcctagt gcaactaata atccattgat gatcatcacc acaaacgcaa tgacaaatgt
     6301 tgggatcaga gcagtgaaag aacgagaaac tcctggcgga acaacatctg gcatttttat
     6361 gacccaattt ctcttgacac acatacaata caattcgacc gcaataatcg acataataat
     6421 agctgtaaag attcctgaag ttcctaaacg ttcgacaaaa gagcccatcc taataccatt
     6481 tactacagtt tctgtctcat tgatagaagc aatcaagctg attttaccat cagtcatgat
     6541 caattcaggg atacacataa agaatgcaaa aacagatagt aatgcccccg tgagcggatt
     6601 aacatttaat ttttgttcat ctcgttcaat tgacgttaat tcataagcaa acacaatggc
     6661 aaagtaaata gataaaatcc ccatcgtcat tgtattgacg atcatgtaca agtcactaac
     6721 tttgaaaaaa gttgcattaa aaaatccttc taaaaatggg aacggtaatg gcaaaatatt
     6781 taaaaccaaa aacatcgagc ctacaatcgt gaaaggaata gaagccatcc ccgctgccat
     6841 gacagcccga acgattttaa gttgcgcaat tttccccata ggtcccatca ggtatttctg
     6901 caaaaaatca aacattagtt attctccttt cctaaatata acgctttttc atattgttta
     6961 atacggatat actgttggtc ttggttgagt gaaattttct ttaaacgata ctgtataaat
     7021 gcactggcaa aaataccgct tacaacaaga atgaaaacaa ataactgtcc tccattgtta
     7081 tttgccataa aaggatacaa gaatgtaaat cctgaggtaa acaacactgg aatcactaat
     7141 aaattaaaga cactctggaa caaaaaatac cactttgtcc atggaacgtt cgtttggtgt
     7201 tttcgataaa gctttacttg ttctcctact gcaggcaaaa tagctaaaat cagaatcatc
     7261 ggtaaactac ttcctaaact tttagctgat agaagcagaa tcatccattg catgttcatg
     7321 aaaaagaaaa aggcagtcag gtaacgtacg attagataac gattgaaata catgtttttc
     7381 aaactaagtt gctttttttc taattcgatt ttccttttta ctgtatcctg cacaatgatc
     7441 cttcctttct atttattttt ttgatacaaa taattcattt cctgtgcaac ttccaataat
     7501 gtcatcgtcg tcatcagatg atcttgtgca tggaccatga taatttccat ttctattttt
     7561 gtcccgctgg catattcttt cagaagttga gtttgtgact gatgcgcatg aagaatctct
     7621 tcatttgctt gtcgcaactt ctcttctact tcttcaaaca tctctttacg catcgagcga
     7681 aatgcttcat gtatcagtgt ccgtgcattt ccgctatgga aaatgatatc aaaagaagtc
     7741 acttgtagtg tttcactgtt tattttttca gccatctgtt ttcctcctta ttcgatcagt
     7801 ttgataaatt tactgctgaa attctcaaaa ataggttcag ctaagatttc ctgctgtact
     7861 tttagctggt caactaacct aacgattgct ttagtcacga ccgtaattcc ttcattttcg
     7921 acataagaag gcgaaactaa aaaaacaaaa tgaatttctt gatattgatc ccactgcatc
     7981 ccagaaggaa tcaatgcaac ggctaccttt gtttcgattc caactggaac agcgggatgt
     8041 gggactgcca ctgtatcact gaatatgatt tgtcccatat tttcacgttg ctggatctgc
     8101 tttcgcattt ctcttatata tccttcttct tcatttatgc tcagcttttg cagcaaatca
     8161 tgaagaattt cttccttttt cggttcgtac gtataagtct tgaaaaattt ttcagacaac
     8221 tgttcggtgt aaatctgctt tttttgctgt agagaaaatg ctggtatcgg tctatctgag
     8281 gaatgatagg gtatagattc tccaactacc tttcgtattt tacgaacatc atcttcattc
     8341 aaaaaaatac tgacatgtaa aactggaatt ttgaaaaaca ttgtcgaaag atcaatagaa
     8401 gaaataatca ggtcgattcc ttttaaagtt gtttcattga tctcataata gccttttaca
     8461 cttaccactg taatattttt accgaattca ctgactacgc gattctttaa tagttgggca
     8521 ctgccgtaac ccgttgcaca gataatcaat gcatgtagtt tatgcgcagc tctttctttt
     8581 tccaatgccg ccatgagatg caaagccaaa taggcccatt catcttcatt gatggtatag
     8641 tttttcaaag ccggcatgtt gctgaaatgc ctttttactg cgtcaaagac tttgggattt
     8701 tctttcttta tctcctcagt caatgggttc tcaatgcgaa tacctcggct caatcgaatc
     8761 atcatcggtg acaaatgatc gagtagtcca ttgaaaagct ggagatcctc tactaatgaa
     8821 aaatgaaatt cctgagacaa tcttttcaga actttcgcta attcctttga tagttcctga
     8881 tcattttgat tttctttgtg attcgactta gccatgagat gtaatgtcaa ataagcaatt
     8941 tcttcttcgg gaaacgatac acctgcgatt acctcaatac gttgaacgat ccgctgcgcc
     9001 acttgatact ctactcgctt agaaacttct tgttcgatgc ctaattcctt gatttcaaac
     9061 ccttctcgga gccgtttgat ccccaaagcc aagtgtaaag tcagattctg tatgataaaa
     9121 tccgaaactt ttaacttagc ttctcttgtt tcatcgagaa tgataatgac tagctcttca
     9181 aaactgatgt ctgggaaaaa acggctttgc cctaaatatt ctttcaatga atttccataa
     9241 tggtttccaa aaaaagtatc catgataaaa tgccgtcgat ttctttcttc cccttcgatc
     9301 catgtccctt gaccatgctt agaaacgatt tttagtgaat atggaatcaa tcgctccttg
     9361 atttcttgca tgtcctttgt caaactggaa cgactgatat aaagctcttc tgccaaatca
     9421 tccataaaaa gaatggcatc ttccaaaagc aattggttca atatatactt ttgtcgatct
     9481 tcggactcat aaaatccttg cggttctcta gcatatccag gtactatttc ttgccgactc
     9541 aaaaacaagt caaatgtcag tctttgtaaa atatgcagtt ggtagccata acctggttta
     9601 gcaatgatct tacctccgtt tttttctact aactctttga tccgctgcaa gtaattacgg
     9661 attgtccgat cagataataa caattcagaa gctaaacgtt gactggtgac atactccttc
     9721 ttgtgatcaa tcaaatatct caacagcaat ttttccttct gtttcattat catccactcc
     9781 tattccatca tatgttctct tcgatcacct ctgctaactt ctggatgcct gtagggattg
     9841 ggacatacgc ttggaaaggg atactaacaa caggctttcc aacagattct cctaattttt
     9901 tgaatttatc caaaaacatc gtcgtttgcg ggctgatcag aaataaatca aaatcactat
     9961 tcttaatcat attatcgcct tctgtagctg aaactgcatc tacgagtacc tcttctcttt
    10021 tttgcttaaa gaaatccgtt gtctttgagg ccatcatcga ggatgacatt cctgccgcac
    10081 aaataatcaa tgcttttttc ataaatcaat cactcctttt atttttataa accaagcata
    10141 tcttgtaagc gttttctatt caaaatatat atttccgtag cattgcggaa aaaatgtgta
    10201 tcatatcttg aataataatt tctggcaccg tgatatacta gtgatagaat tttttcagga
    10261 ggggatttag tgagtatcac agtgaatatt ttgtatacaa gtacaaatgg acaagccaga
    10321 aaatttgcag aagaaatggt cgaaagaaac attgtcaaag cgattcgtgc agaagaagga
    10381 aacgagaaat atgactatta ctttccacta gaagatccag aatctctttt actgatcgac
    10441 cgttggaaag atgaagaagc aatcgaaaag catcacaaat ccgaaatgat ggctcaaata
    10501 aacgaactcc gtaaaaagta taaattaaaa atgaaagtcg aacgtttcac tgatttaccg
    10561 taaaaagata cacatggaat gatagcaatt tatcatccat gtgtatcttc actttttcct
    10621 tattttttat tcgatctcat ctaatgattc aaaccgtgtt tttctcttat ttttcggata
    10681 agtatttcag tcaccccaat atacatgatt cctaaacata ccccaaggat attctcaaaa
    10741 atgcggatcg ttactgctcc tggaaccccg aataaactgg cagtgattgc caaagcacca
    10801 aaagaattaa aaactgtttt atatctatag gtagaacaaa cacctaaagc tagaccacct
    10861 aatataccga cccagttaaa aggaataaat tggctgatac caataaataa aacacatcca
    10921 atcaatgagc caatgattcg gtctactgct cgttctttga aaccaatcga catcaatcca
    10981 tagctagata caatagaaga aaatgcaaac cctgcccaca tcaatcgctg aaaagggaca
    11041 tactctcctg caacgatcaa aagactaatg ccaaatgcat agatgagcat ccaaattttt
    11101 tcctgcgaat acatgccttt caaaaaaatc tctttaaaca agcttttgtc tttgttcttt
    11161 tctcgatgtt ttctgtaaag gagaactgaa aaccaacaaa aaaacaaaaa taataagcta
    11221 cttgtttgtg taaagaaatt cttgttcaac gaatctttag gtaacgaata aacaataaag
    11281 agatatgaaa agccgtacaa agaagcattc cccattttgg gatcactagc ggttgccatt
    11341 aatatgctgc ttagtaaaat aaaatgaatc aacatgccca taactgacca tttgatcagc
    11401 tggacatacg gtgctactaa tagtgagaac atgacgattg ctaaacctat gatcgaatgg
    11461 gaaatcttat aaccaaagtc aacaaaacga atactcatca acatacagaa taaaaccaca
    11521 gcgtatggtt tatgcttcga ttcaaataat gttgtaatta taatgataaa acaaatggca
    11581 aacaatacga tgaataatga tcgtaaaaac aatgaacaaa tatacatgca cttttctttt
    11641 ttagaagctg cttctcgtat tttttgtttt aaaataaacg gatctagttg tagagactga
    11701 taaaaatcca cctgttgtcg tcctttcttt ttacttttat tttttacagt catctatgac
    11761 aggttgtaaa tactatggaa catcatatgg cgttgtcatt ttcttgtcaa ctatttttcc
    11821 tattcatgtt tttataaaaa aacatatcgt tttcttgtat gattaaatca ccacaaaata
    11881 aacaactgag agaacgatat gctttatgaa tggttctatc aaaaaaattc tgagatcaat
    11941 agacaaaaat ttgatgatta cagaggtctc ttacgaaacc ttacagaaga aaaaaacatt
    12001 gatcgttgct gcagttctct tccctgctcc atgtgtctgt agaaattgtg gttctgctgt
    12061 ggtagatgga aataggaaat taatggtggt gaaaaacaag aagaaggaaa ccattgtcca
    12121 ctttgaataa tataatcata tgccaatggt tatacgccta aaaaagcagc gctatacctg
    12181 taaaaactgt cgaagccatt ggaacgctca aagttatttt atccgtccaa ggcactcgat
    12241 ttcaaatcat gtcagacata aaatcacttc tttactgact gaaaaagtgt cgttattttt
    12301 tatttcgaaa agttgttagg tatctttgac aaccgttatt cgcacattga aagagttgag
    12361 aagctattta ccaaagtctt ccaaaagaat tctgtcttga gtgttgaggg ttgtgaattt
    12421 cgttctcacg tttccgttga ggataaactg agttttattt ccgcggatgg cgagacagaa
    12481 aaattaatcg agaaaattta cctacacaaa aaattcctcg attaacaaac tatttcctta
    12541 gctgcactaa tcctaaagaa atagaattct tagttacaga catgaacgcc acctactttc
    12601 agctcaccaa acatgttcta ccaaatgcga aattggtgat tgatcgatta cttggttttt
    12661 ccacgccttt caaggaggcg taacctttat tcacgagttg gttgaagctt tccgagacaa
    12721 aagacccgac ttgtttttct ccttattgac aaagagccat taaaactaga gaaaaaatta
    12781 caactgatcg ctgattattt gtagaaagaa aatcaaagta ttacccaata aacttgagtc
    12841 attaggcaaa aaacactgcg aaactcaaga atgattttcg cagtgttttt gtatattttt
    12901 tattaactag tattactaca aaggtttatt tttcacttct caacgcagca caaaattgtc
    12961 tttacttatt tactggttgg gttcaacctt ctctactccg atactaatat ttgtttgtat
    13021 accccttcta aaatcggtag tttccattca attgatgggt actagttgat tttaaaaact
    13081 atgcttattc aattttttat tttagctcat atatttcgtt tctttctaaa ttttatactg
    13141 atgaaaacaa gtagcacacc caatattgac aatataaact ggctaatgat ggtttctcca
    13201 gtttttggaa gatgcttatt tccactgctt gttggattgt ttcctcttat atctgagcta
    13261 tcagcaactt ttctattctc ttttgttaaa aagatcgcca cttcttgttc tttttcgatc
    13321 ttgaaagtta ctggtgtcgc atccaattca taccccgttg gcgcttgagt ttctaccagc
    13381 tgatatgctc ctggtgctaa gccatctatc gccagttttc catctgctcc tgtggttaga
    13441 cctgactgca gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcaggact
    13501 tctccgcttt ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag tcgattttct
    13561 ttcgtcaatt ctaccaccgc tgtctgactt ctttcgatct caaactcgat tggtgtcgca
    13621 tccaattcat accccgttgg cgcttgggtt tctaccagct gatacactcc tggtgctaag
    13681 ccgtcaatcg ccagttttcc atctgctcct gtggttagac ctgactgcag aacggttcca
    13741 ttggcatctt gtaactcaaa aacagctcct tgcaggactt ccccgctttg gtcatcgatc
    13801 tttgtcaaca cgacaccgcc tggtgtaagt cggtttgctt tcgtcaattc taccaccgct
    13861 gtctgacttc tttcgatctc aaactcgatc ggtgtcgcat ctaattcata ccccgttggc
    13921 gcttgagttt ctaccagctg atatg
//
LOCUS       DAZKOM010000073        13917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000073 DAZKOM010000000
VERSION     DAZKOM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.073"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8NF_002023"
     CDS             131..505
                     /locus_tag="ACG8NF_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0472496.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8NF_002024"
     CDS             complement(619..1248)
                     /locus_tag="ACG8NF_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0472497.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8NF_002025"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8NF_002025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8NF_002026"
     tRNA            1668..1741
                     /locus_tag="ACG8NF_002026"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACG8NF_002027"
     CDS             complement(1879..2043)
                     /locus_tag="ACG8NF_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472498.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG8NF_002028"
     CDS             2338..3666
                     /locus_tag="ACG8NF_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0472499.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTVVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACG8NF_002029"
     CDS             3868..4701
                     /locus_tag="ACG8NF_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0472500.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACG8NF_002030"
     CDS             4717..5454
                     /locus_tag="ACG8NF_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0472501.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACG8NF_002031"
     CDS             complement(5609..6577)
                     /locus_tag="ACG8NF_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0472502.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACG8NF_002032"
     CDS             6809..7642
                     /locus_tag="ACG8NF_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0472503.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACG8NF_002033"
     CDS             7676..8515
                     /locus_tag="ACG8NF_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0472504.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACG8NF_002034"
     CDS             8541..9101
                     /locus_tag="ACG8NF_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0472505.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACG8NF_002035"
     CDS             9300..11021
                     /locus_tag="ACG8NF_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0472506.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACG8NF_002036"
     CDS             11186..12328
                     /locus_tag="ACG8NF_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0472507.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8NF_002037"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8NF_002037"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0472508.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2832 g         4036 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cgttgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAZKOM010000074        13732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000074 DAZKOM010000000
VERSION     DAZKOM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.074"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACG8NF_002038"
     CDS             complement(143..1345)
                     /locus_tag="ACG8NF_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0472509.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACG8NF_002039"
     CDS             complement(1348..1542)
                     /locus_tag="ACG8NF_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0472510.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACG8NF_002040"
     CDS             complement(1589..2437)
                     /locus_tag="ACG8NF_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFQ0472511.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACG8NF_002041"
     CDS             complement(2642..2992)
                     /locus_tag="ACG8NF_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472512.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACG8NF_002042"
     CDS             3170..4033
                     /locus_tag="ACG8NF_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0472513.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACG8NF_002043"
     CDS             4657..5649
                     /locus_tag="ACG8NF_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0472514.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACG8NF_002044"
     CDS             5665..6549
                     /locus_tag="ACG8NF_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472515.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACG8NF_002045"
     CDS             6598..8205
                     /locus_tag="ACG8NF_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0472516.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACG8NF_002046"
     CDS             8206..9690
                     /locus_tag="ACG8NF_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0472517.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACG8NF_002047"
     CDS             9867..10859
                     /locus_tag="ACG8NF_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0472518.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACG8NF_002048"
     CDS             10911..11780
                     /locus_tag="ACG8NF_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0472519.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8NF_002049"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8NF_002049"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0472520.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAZKOM010000075        13645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000075 DAZKOM010000000
VERSION     DAZKOM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.075"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..263
                     /gene="yaaA"
                     /locus_tag="ACG8NF_002050"
     CDS             21..263
                     /gene="yaaA"
                     /locus_tag="ACG8NF_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0472521.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            250..1374
                     /gene="recF"
                     /locus_tag="ACG8NF_002051"
     CDS             250..1374
                     /gene="recF"
                     /locus_tag="ACG8NF_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0472522.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1371..3317
                     /gene="gyrB"
                     /locus_tag="ACG8NF_002052"
     CDS             1371..3317
                     /gene="gyrB"
                     /locus_tag="ACG8NF_002052"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0472523.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3337..5808
                     /gene="gyrA"
                     /locus_tag="ACG8NF_002053"
     CDS             3337..5808
                     /gene="gyrA"
                     /locus_tag="ACG8NF_002053"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0472524.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6044..6343
                     /gene="rpsF"
                     /locus_tag="ACG8NF_002054"
     CDS             6044..6343
                     /gene="rpsF"
                     /locus_tag="ACG8NF_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0472525.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6393..6905
                     /gene="ssb"
                     /locus_tag="ACG8NF_002055"
     CDS             6393..6905
                     /gene="ssb"
                     /locus_tag="ACG8NF_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0472526.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6931..7167
                     /gene="rpsR"
                     /locus_tag="ACG8NF_002056"
     CDS             6931..7167
                     /gene="rpsR"
                     /locus_tag="ACG8NF_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0472527.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7310..9283
                     /locus_tag="ACG8NF_002057"
     CDS             7310..9283
                     /locus_tag="ACG8NF_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0472528.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9289..9741
                     /gene="rplI"
                     /locus_tag="ACG8NF_002058"
     CDS             9289..9741
                     /gene="rplI"
                     /locus_tag="ACG8NF_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0472529.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10015..11382
                     /gene="dnaB"
                     /locus_tag="ACG8NF_002059"
     CDS             10015..11382
                     /gene="dnaB"
                     /locus_tag="ACG8NF_002059"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0472530.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12162..13454
                     /locus_tag="ACG8NF_002060"
     CDS             12162..13454
                     /locus_tag="ACG8NF_002060"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0472531.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4542 a         2475 c         3007 g         3621 t
ORIGIN      
        1 atgaaaatga gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt
       61 tgggccaaat gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt
      121 tggcagaaaa cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt
      181 atcccggcat gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa
      241 acgacgataa tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg
      301 aacatcgaat tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg
      361 aatcttttgg aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag
      421 caggaattga ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt
      481 tcatccactc ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac
      541 catatcgaac agaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca
      601 ccagaagatc tttctttagt aaaaggcagc ccgcagatca ggcggaagtt tttagatatg
      661 gaattaggcc agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg
      721 aaacaaagaa accagtactt gaagcaattg aatgaaaaaa aacaaactga tttgctttat
      781 ttggatattt tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca
      841 cagttcgtca agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa
      901 aaagagcagc tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa
      961 gagcaaatcc aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt
     1021 cggggaacaa cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa
     1081 aatgtgcaga cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta
     1141 gcagaaatcg atttgattaa agaagagaca ggggaatatc caatcctttt gttagatgat
     1201 gtgatgagcg agctggatga taatcgtcaa ctccatctgc tagaaacaat cgaaggaaaa
     1261 gttcaaacgt tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa
     1321 cctgagattt tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag
     1381 aaagaagttt agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag
     1441 gacttgaagc cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc
     1501 tgcatcacct tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg
     1561 caacgaaaat ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc
     1621 gggggatacc tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta
     1681 ctgttttaca tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc
     1741 acggagtagg atcttctgtt gtaaacgcgc tttctacaca gcttgatgtc aaagtataca
     1801 aagaaggaaa tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga
     1861 ttatcgaaca aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt
     1921 ttacagagac aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt
     1981 tcttgaaccg cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca
     2041 atgaatacca ttatgacggc gggatcaaaa gttatgtcga atacttgaat gcaaacaaaa
     2101 ccgtgatttt tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag
     2161 tctctatgca atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc
     2221 atacgtatga aggtggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa
     2281 atgattatgc ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag
     2341 acgtgcgaga aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag
     2401 gacaaacaaa aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta
     2461 gcgaacattt catgaaattc ttgctggaaa acccaagtgt gggccggcaa attgttgaaa
     2521 aaggattact agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac
     2581 gtaaaggtgc tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg
     2641 atcctgaaaa atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc
     2701 aaggtcgtaa tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg
     2761 aaaaagcaag catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta
     2821 tgggtacagg gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta
     2881 tcatgactga tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt
     2941 atcgttacat gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat
     3001 acggggtaaa acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag
     3061 agttgaaaca agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata
     3121 aaggacttgg agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc
     3181 ggatgatggc ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt
     3241 tgatggggga tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga
     3301 agaacttaga tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac
     3361 atccatgatg tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt
     3421 gttattgtcg cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt
     3481 attttatacg gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc
     3541 agaatcgtcg gagatgtcat gggtaaatac catcctcatg gagacattgc gatctatgag
     3601 tcaatggtac ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga
     3661 aactttggtt ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg
     3721 agcaaaatcg ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc
     3781 aactatgatg atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta
     3841 gtgaacggaa caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg
     3901 tctgaggtag tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa
     3961 ttgatggaag tattacctgg accagatttt ccaacaggtg gtttggttat ggggaaatca
     4021 ggaatccgaa gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag
     4081 attactgaga tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc
     4141 aataaagcaa aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg
     4201 atcacagatt tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt
     4261 cgtgacgcca gcgcatccgt tatcttgaat aacttatata aattgacatc cttgcaaaat
     4321 tcatttggat tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa
     4381 caaatccttg aaaactatat cgaacatcaa cgcgaagtca tcacacgacg gacggtcttt
     4441 gaaaaagaaa aagcagaagc aagagcacac atcttagaag gtttgcgtat tgctttggat
     4501 catatcgatg aaatcattgc gattatccgc gggtctaaat cagatgacga ggcaaaagca
     4561 acaatgatcg aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt
     4621 ttacgccgat tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta
     4681 aaactgattg aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc
     4741 aagaatgaat tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta
     4801 gtcggtgagg tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt
     4861 acattgacaa acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga
     4921 aggggtggac gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg
     4981 gtttcttgtt ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg
     5041 gcaaaaggat acgaaattcc agaatacggg cgtacagcca aaggaatccc aatcatcaat
     5101 ttactaggta tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca
     5161 gaagaaggcc actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc
     5221 actgcttttg ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat
     5281 gagctcgtca atgtgctact gacagatggt caatctaata tcattattgg aacacataac
     5341 ggctactctg tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg
     5401 gtcagaggga tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa
     5461 aatcaagaag tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa
     5521 tatccagtga aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat
     5581 ggtccactag ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat
     5641 aaaggtgtca ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta
     5701 ggcgtacgtt tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa
     5761 ccagaagctg cagaacaaac agttgaagaa caaggatcag aagaataacc atcaagacta
     5821 agtgtgtaag ctagcaagaa taaccgatta aaaaatctgg ttattcctgc tggctttttt
     5881 gattagttga atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa
     5941 aatagagtgt ataattagag cttgtgagta gtctaattaa ttagataact ctccttgctc
     6001 ttaggaacct aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata
     6061 tgaaatcatg tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga
     6121 acgtttcgat gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga
     6181 aaaacgccgt ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa
     6241 agtatcttct ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa
     6301 cgacgatatc attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa
     6361 acaatagaaa acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac
     6421 gtctgacgaa agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta
     6481 ctttggctgt gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca
     6541 actgtgtgat ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac
     6601 ttttaggtgt tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg
     6661 tatacgtgac agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg
     6721 aaaatcgcca acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg
     6781 gaaacaataa ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg
     6841 atacttctga cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat
     6901 tctaaacgaa tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa
     6961 cgtcgtaaag tagactacat tgctgctaac catattgaat atatcgatta taaagatatc
     7021 gaattactaa aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt
     7081 acaggcgcta aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc
     7141 ttgctacctt tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg
     7201 atcagctatt caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa
     7261 tgacgaaagt tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa
     7321 aaacaatcat ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt
     7381 ggtagtctta attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt
     7441 atggggtgtt cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa
     7501 aatccagcag gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc
     7561 tgtaggagtg attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc
     7621 attgaaaatc aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac
     7681 tgcaaaagaa aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat
     7741 ggatacagta aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact
     7801 ccatcagacg aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc
     7861 cattgataaa ttagatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt
     7921 ttctgactgg atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt
     7981 cttcgtagca cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga
     8041 tcgctttcga gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc
     8101 ttatggctca agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc
     8161 tttagtccgt ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt
     8221 ttattatggg gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat
     8281 gagtaccgct ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa
     8341 gtttccggat atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa
     8401 ccagaaaaaa gcttacatcg ttatcaatga gaacgagatc attccagatg tagaacggtg
     8461 tcttgaagaa atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc
     8521 catagagctg aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct
     8581 atcgatctct cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg
     8641 tcgtggggaa gagtttccaa gtaaacctct gctgacttat attgaatctt cagcttcttc
     8701 agcttcagag ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga
     8761 tcgttttgag gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt
     8821 ccgtacaact gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc
     8881 atccttagta caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt
     8941 gattgctgga agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa
     9001 agagtataat agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat
     9061 caatgcagct ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag
     9121 taccggcacg atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac
     9181 aaacgcagct actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttgc
     9241 tgcaatcaaa gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt
     9301 tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt
     9361 tatgcacaaa attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc
     9421 agtcagttag caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa
     9481 gctaaaaagt taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa
     9541 gcaggggaag acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta
     9601 aacaaacaat acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt
     9661 tcactaggct acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa
     9721 gttcatgtcg tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca
     9781 gaaggaaaaa agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa
     9841 gctgcaaatc gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa
     9901 agaaaacgag tataataaaa gagaactatt tttttattac actttcggta cgaatgatga
     9961 cttttctctc attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat
    10021 gaagtgtggc aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc
    10081 tctgtttttc ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac
    10141 ttttatcgtc gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat
    10201 gaagcgattg acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat
    10261 gccggtggtt taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc
    10321 gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca
    10381 acaggaatcg ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat tttagatgat
    10441 gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt
    10501 gctgaagtac tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa
    10561 atcacaggtc taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc
    10621 gaagaactaa tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat
    10681 attgctcaaa atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg
    10741 ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat
    10801 ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt
    10861 ctttctcgag caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt
    10921 gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat
    10981 ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct
    11041 cgccaactga aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta
    11101 tctcgtggag tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc
    11161 ggatcaattg aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa
    11221 agagaaggcg gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc
    11281 atcatcgaga agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa
    11341 tacaataagt tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct
    11401 tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag
    11461 tacttaattt aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt
    11521 tccgaataaa cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata
    11581 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac
    11641 ctcttttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca
    11701 aacatgagaa gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca
    11761 caacctcttt cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac
    11821 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
    11881 ttcacaacct ctgattcacg gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt
    11941 tcacaaaaat ttgagaagca attttcgtac attccttctt atttcttgaa gctgaccact
    12001 tctgttacaa cctctttttt aataaccatt ctaaaaagat aaagttcgtt ttttaagcgt
    12061 ttttataaaa taaaaataaa aatcattcga ttttatttga aatatgtatc tattattgat
    12121 acaatggttc aggattaaat aatatagaac gaggtgttca catgtcatct gtagtagtag
    12181 taggtacgca atggggcgat gaagggaaag gaaaaatcac agactttctc agtgaaaatg
    12241 ctgaagtgat tgcacggtac caaggtggcg acaatgcagg gcacacaatt aagtttaacg
    12301 gtgtgactta taaactgcat ttgattcctt ctgggatttt ttataaagac aagatcagtg
    12361 tgatcggaaa cggcgttgta gtcaatccta aatcattggt taaagaatta gcttatttac
    12421 atgaaaaagg tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac gtaatcttgc
    12481 cttatcatat caaattggac caattacaag aagatgctaa gggcgaaaat aagattggta
    12541 caacgattaa aggaatcgga cctgcctata tggataaagc agctcgtgta ggtatccgta
    12601 ttgcagactt attagataaa gaaatttttg aagaacgtct tcgtttgaat ctagatgaca
    12661 aaaaccgtca atttgtaaaa atgtttgatt cagaaccact tgattttgac gatatttttg
    12721 aagaatatta tgaatacgga caacaaatca aacaatacgt cacagatact tccgttatct
    12781 tgaatgatgc tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa ggtgtaatgc
    12841 tggatatcga ccagggaact tatccttttg ttacttcttc taacccagta gccggtgggg
    12901 taacaatcgg aagcggtgta gggccttcaa aaatcaataa agttgtcggc gtctgcaaag
    12961 cctatacgtc acgtgtcggt gatggaccat ttccaactga attatttgat gaaacaggag
    13021 aacaaatccg cgaagtagga cgagaatacg gtacgacaac tggtcgtccg cgtcgtgtag
    13081 gctggtttga ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt accaacctat
    13141 ctttaaactc gatcgatgta ttaagtggat tggaaacagt aaaaatttgt acggcttatg
    13201 aattagatgg cgaactaatc taccactatc cagcaagctt gaaagagttg aaccgctgca
    13261 aaccaatcta tgaagaattg ccaggctggt cagaagacat tacaggatgc aaaacacttg
    13321 cagaactgcc agaaaacgca cgtaactatg tacgtcgtat ctcagagctt gttggtgtac
    13381 gtatctcaac attttctgtc ggacctgata gaacacaaac aaacatctta gaaagcgtct
    13441 gggcgcaaat ctaaggataa aaacgacgac tgaatcagga cgttcttacc agagcgagtc
    13501 gaagtgttta tctatacgat aataataact tgaagaggta gtgaaagaga cgtttagcct
    13561 tattgccgat aggctgtctc ttgaacactg tttattcaga ataagggact gtgacaaaaa
    13621 ccgtgtcaca gtcccttttt ccgaa
//
LOCUS       DAZKOM010000076        13305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000076 DAZKOM010000000
VERSION     DAZKOM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.076"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8NF_002061"
     CDS             complement(105..629)
                     /locus_tag="ACG8NF_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0472532.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8NF_002062"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8NF_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0472533.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8NF_002063"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8NF_002063"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0472534.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8NF_002064"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8NF_002064"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0472535.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8NF_002065"
     CDS             3157..4026
                     /locus_tag="ACG8NF_002065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0472536.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8NF_002066"
     CDS             4052..4567
                     /locus_tag="ACG8NF_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0472537.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8NF_002067"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8NF_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472538.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8NF_002068"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8NF_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472539.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8NF_002069"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8NF_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472540.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8NF_002070"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8NF_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0472541.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8NF_002071"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8NF_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0472542.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="ACG8NF_002072"
     CDS             complement(7873..9090)
                     /locus_tag="ACG8NF_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0472543.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="ACG8NF_002073"
     CDS             complement(9348..10625)
                     /locus_tag="ACG8NF_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472544.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8NF_002074"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8NF_002074"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0472545.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="ACG8NF_002075"
     CDS             complement(11547..12491)
                     /locus_tag="ACG8NF_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0472546.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="ACG8NF_002076"
     CDS             complement(12493..13179)
                     /locus_tag="ACG8NF_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0472547.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3783 a         2776 c         2397 g         4349 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    13261 agacactatt tttaaatatt tttcgttatt tttttatcac aaaaa
//
LOCUS       DAZKOM010000077        13268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000077 DAZKOM010000000
VERSION     DAZKOM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.077"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..1971
                     /gene="yfcC"
                     /locus_tag="ACG8NF_002077"
     CDS             232..1971
                     /gene="yfcC"
                     /locus_tag="ACG8NF_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0472548.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2128..3312
                     /gene="iadA"
                     /locus_tag="ACG8NF_002078"
     CDS             2128..3312
                     /gene="iadA"
                     /locus_tag="ACG8NF_002078"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0472549.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3423..3626
                     /locus_tag="ACG8NF_002079"
     CDS             3423..3626
                     /locus_tag="ACG8NF_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0472550.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3727..4389)
                     /locus_tag="ACG8NF_002080"
     CDS             complement(3727..4389)
                     /locus_tag="ACG8NF_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472551.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4402..5904)
                     /locus_tag="ACG8NF_002081"
     CDS             complement(4402..5904)
                     /locus_tag="ACG8NF_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0472552.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6452..8131
                     /locus_tag="ACG8NF_002082"
     CDS             6452..8131
                     /locus_tag="ACG8NF_002082"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0472553.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8258..8737
                     /locus_tag="ACG8NF_002083"
     CDS             8258..8737
                     /locus_tag="ACG8NF_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0472554.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8956..9636
                     /locus_tag="ACG8NF_002084"
     CDS             8956..9636
                     /locus_tag="ACG8NF_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0472555.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9644..10975
                     /locus_tag="ACG8NF_002085"
     CDS             9644..10975
                     /locus_tag="ACG8NF_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0472556.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10990..11952
                     /locus_tag="ACG8NF_002086"
     CDS             10990..11952
                     /locus_tag="ACG8NF_002086"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0472557.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12063..12401
                     /locus_tag="ACG8NF_002087"
     CDS             12063..12401
                     /locus_tag="ACG8NF_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0472558.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12480..13187
                     /locus_tag="ACG8NF_002088"
     CDS             12480..13187
                     /locus_tag="ACG8NF_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0472559.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4252 a         2329 c         2896 g         3791 t
ORIGIN      
        1 tctctaatct aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat
       61 ataaatggaa agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag
      121 tgattttcta ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt
      181 atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa
      241 acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat
      301 gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga
      361 aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact
      421 gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat
      481 caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa
      541 ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa
      601 atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct
      661 aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc
      721 ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc
      781 gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc
      841 gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg
      901 ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt
      961 ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca
     1021 gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt
     1081 agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga
     1141 ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa
     1201 aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct
     1261 catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta
     1321 ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac
     1381 attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt
     1441 cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca
     1501 gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg
     1561 ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg
     1621 gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg
     1681 ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct
     1741 ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt
     1801 tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt
     1861 gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt
     1921 gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga
     1981 cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg
     2041 gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag
     2101 ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca
     2161 ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa
     2221 gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa
     2281 gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc
     2341 ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg
     2401 acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc
     2461 aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga
     2521 ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt
     2581 atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag
     2641 ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc
     2701 atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa
     2761 gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta
     2821 tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa
     2881 gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga
     2941 ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc
     3001 ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca
     3061 ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta
     3121 cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa
     3181 attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt
     3241 atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa
     3301 aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct
     3361 gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca
     3421 caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa
     3481 aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat
     3541 cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt
     3601 tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa
     3661 aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc
     3721 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
     3781 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
     3841 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
     3901 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
     3961 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
     4021 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
     4081 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
     4141 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
     4201 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
     4261 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
     4321 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
     4381 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
     4441 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
     4501 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
     4561 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
     4621 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
     4681 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     4741 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     4801 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     4861 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     4921 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     4981 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     5041 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     5101 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     5161 catacccata ctgccgcttg tttctctgga ttcataaatt cctttgatcg tcatttcata
     5221 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     5281 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     5341 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     5401 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     5461 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     5521 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     5581 tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     5641 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     5701 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     5761 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     5821 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     5881 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     5941 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     6001 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     6061 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     6121 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     6181 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     6241 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     6301 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     6361 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     6421 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     6481 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     6541 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     6601 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     6661 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     6721 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     6781 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     6841 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     6901 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     6961 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     7021 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     7081 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     7141 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     7201 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     7261 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     7321 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     7381 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     7441 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     7501 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     7561 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     7621 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     7681 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     7741 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     7801 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
     7861 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
     7921 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
     7981 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
     8041 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
     8101 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
     8161 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc
     8221 gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga
     8281 acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga
     8341 agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac
     8401 tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca
     8461 cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat
     8521 ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa
     8581 actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt
     8641 aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa
     8701 gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca
     8761 gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc
     8821 tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta
     8881 tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa
     8941 cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg
     9001 gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta
     9061 actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata
     9121 ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt
     9181 gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat
     9241 gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta
     9301 gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa
     9361 acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa
     9421 ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat
     9481 gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact
     9541 ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga
     9601 aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga
     9661 tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat
     9721 cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa
     9781 tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa
     9841 aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt
     9901 aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa
     9961 acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga
    10021 agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac
    10081 gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta
    10141 tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa
    10201 agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca
    10261 gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc
    10321 agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa
    10381 atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac
    10441 aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt
    10501 cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat
    10561 gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa
    10621 tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt
    10681 cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga
    10741 aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat
    10801 tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg
    10861 tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga
    10921 aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc
    10981 aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc
    11041 attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc
    11101 gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa
    11161 gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta
    11221 gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca
    11281 gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg
    11341 gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct
    11401 gaatctttaa agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat
    11461 acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg
    11521 agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa
    11581 gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga
    11641 agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct
    11701 tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag
    11761 cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg
    11821 gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta
    11881 gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga
    11941 aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc
    12001 ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga
    12061 agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct
    12121 tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag
    12181 aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga
    12241 cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag
    12301 tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg
    12361 gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt
    12421 cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa
    12481 tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat
    12541 tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag
    12601 tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac
    12661 agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag
    12721 gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag
    12781 gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga
    12841 agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga
    12901 ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt
    12961 tttctttagc attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc
    13021 gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa
    13081 gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc
    13141 agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg
    13201 tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt
    13261 tggaataa
//
LOCUS       DAZKOM010000078        13212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000078 DAZKOM010000000
VERSION     DAZKOM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.078"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8NF_002089"
     CDS             147..1016
                     /locus_tag="ACG8NF_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0472560.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8NF_002090"
     CDS             1225..2250
                     /locus_tag="ACG8NF_002090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0472561.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8NF_002091"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8NF_002091"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0472562.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG8NF_002092"
     CDS             3333..5036
                     /locus_tag="ACG8NF_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0472563.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8NF_002093"
     CDS             5038..7071
                     /locus_tag="ACG8NF_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0472564.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8NF_002094"
     CDS             7082..7522
                     /locus_tag="ACG8NF_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0472565.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8NF_002095"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8NF_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0472566.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8NF_002096"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8NF_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0472567.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG8NF_002097"
     CDS             9793..11559
                     /locus_tag="ACG8NF_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0472568.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG8NF_002098"
     CDS             11574..12011
                     /locus_tag="ACG8NF_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0472569.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG8NF_002099"
     CDS             12024..13175
                     /locus_tag="ACG8NF_002099"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0472570.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZKOM010000079        13202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000079 DAZKOM010000000
VERSION     DAZKOM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.079"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG8NF_002100"
     CDS             complement(<1..205)
                     /locus_tag="ACG8NF_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472571.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACG8NF_002101"
     CDS             complement(221..835)
                     /locus_tag="ACG8NF_002101"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0472572.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACG8NF_002102"
     CDS             complement(982..1665)
                     /locus_tag="ACG8NF_002102"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0472573.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACG8NF_002103"
     CDS             complement(1774..2232)
                     /locus_tag="ACG8NF_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0472574.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACG8NF_002104"
     CDS             complement(2316..4079)
                     /locus_tag="ACG8NF_002104"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0472575.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACG8NF_002105"
     CDS             complement(4300..5760)
                     /locus_tag="ACG8NF_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472576.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8NF_002106"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8NF_002106"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0472577.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACG8NF_002107"
     CDS             complement(6917..8344)
                     /locus_tag="ACG8NF_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0472578.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACG8NF_002108"
     CDS             complement(8375..9589)
                     /locus_tag="ACG8NF_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0472579.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            9939..11018
                     /locus_tag="ACG8NF_002109"
     CDS             9939..11018
                     /locus_tag="ACG8NF_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0472580.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(11319..11561)
                     /locus_tag="ACG8NF_002110"
     CDS             complement(11319..11561)
                     /locus_tag="ACG8NF_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0472581.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11593..12150)
                     /locus_tag="ACG8NF_002111"
     CDS             complement(11593..12150)
                     /locus_tag="ACG8NF_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0472582.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12163..12351)
                     /locus_tag="ACG8NF_002112"
     CDS             complement(12163..12351)
                     /locus_tag="ACG8NF_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0472583.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12364..12930)
                     /gene="amaP"
                     /locus_tag="ACG8NF_002113"
     CDS             complement(12364..12930)
                     /gene="amaP"
                     /locus_tag="ACG8NF_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0472584.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3847 a         2809 c         2312 g         4234 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaggcggatt
     9841 ttataattaa cttagccatt tggaaataaa aaaacgccac gtgaattcca tgttatattg
     9901 aagttaccac aactctcaat agacaaggat gaattcacat gacgcaaaac catgataaca
     9961 cagtttcacg caagggaaaa cacctaacct actcagaacg ttgccaaatc gccatcctga
    10021 aaaaggaaaa ctattccaac cgtcaggttg cggatgtcct gggctgcgtg ccgcagacca
    10081 taaacaacga aatcaaacgc gggaccgtta ctcagcttaa ccgccagaat cagaacggga
    10141 aggtctacga ctattatcat gacgtctacg acgccgatgc cgggcaggcg gcctacgaga
    10201 agaaccgctt gaactgcggc agacggccct tatgggccga cacggacgcc ttcattgaat
    10261 gggcggacga catgatgctg ttggaaaaat ggtccccgga cgtggtggtg ggctttgcca
    10321 gaaaacacga attgttcgac ccggccatta tccctgggac caccaccctc tatcagtgga
    10381 tcgaccgtgg catcatgaaa acgaagaata tggacctgct agaaaaactg tcccgcaaac
    10441 cgaaatcgtc ccgtcatggc aaccgcccga ataaacgggt gctagggaaa tccattgaag
    10501 agcgcccgga ggcggttggt acccggaaaa ccttcggcca ttgggaagtc gacacggtcg
    10561 taggaaacaa gaccaagtcg gatgccgtgc tgctgacgtt ggtggaacgg cagacacgct
    10621 tcgaggtcat tctgaagctg gccgggaagg atgccgaatc ggtcaaccaa gcggtccgac
    10681 agctgagaga acgggctggt gcgtacttcg gccagctgtt caagacggtt acctcggaca
    10741 acggctcgga gttcgcggga ctgcacgatg ccttgcagga tgtggcaaac atctacttca
    10801 gccaccccta tgcttcctgg gagcgcggga cgagcgaaaa ccaacacaag ctgattcgcc
    10861 gtttcatccc caagggcagc ccaatcggca acgtgagcga aaaccaaatc ttgcggattc
    10921 agcgttggat gaacgattac ccgcgaaaga ttttgggcta tgcaacgccg catgacacgt
    10981 tcgtccaggc atttaggcag gaacggttga cggcttgaca cgtgggccct tgacaacgag
    11041 cctccctcgg catggcttgt gtctggaaag ctggccgggg ccagccagtc gggagactac
    11101 catgcctcag ctcattgtca agggcccact cgattagaaa aattagacgg ttggtggtaa
    11161 cgagcaaccg ggtcaccatg ggtggctaac ttaaacttga aatttacgta aggaataaag
    11221 aatacttcat ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat
    11281 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
    11341 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
    11401 attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca
    11461 atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct
    11521 ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg
    11581 acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt
    11641 gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc
    11701 tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt
    11761 gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc
    11821 agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac
    11881 gatcacatct agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt
    11941 tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt
    12001 caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc
    12061 ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc
    12121 atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg
    12181 atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat
    12241 tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc
    12301 aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc
    12361 ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct
    12421 gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc
    12481 gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc
    12541 gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat
    12601 gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt
    12661 tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac
    12721 gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg
    12781 ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat
    12841 atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat
    12901 aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc
    12961 tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta
    13021 aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg
    13081 aaataacaat tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat
    13141 attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta
    13201 tt
//
LOCUS       DAZKOM010000080        13193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000080 DAZKOM010000000
VERSION     DAZKOM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.080"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..980)
                     /locus_tag="ACG8NF_002114"
     CDS             complement(366..980)
                     /locus_tag="ACG8NF_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0472585.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1586..1963)
                     /locus_tag="ACG8NF_002115"
     CDS             complement(1586..1963)
                     /locus_tag="ACG8NF_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472586.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2263..3144)
                     /gene="galU"
                     /locus_tag="ACG8NF_002116"
     CDS             complement(2263..3144)
                     /gene="galU"
                     /locus_tag="ACG8NF_002116"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0472587.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3187..4206)
                     /locus_tag="ACG8NF_002117"
     CDS             complement(3187..4206)
                     /locus_tag="ACG8NF_002117"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0472588.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4839..5843)
                     /locus_tag="ACG8NF_002118"
     CDS             complement(4839..5843)
                     /locus_tag="ACG8NF_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0472589.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5856..6905)
                     /locus_tag="ACG8NF_002119"
     CDS             complement(5856..6905)
                     /locus_tag="ACG8NF_002119"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0472590.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6922..7743)
                     /locus_tag="ACG8NF_002120"
     CDS             complement(6922..7743)
                     /locus_tag="ACG8NF_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0472591.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7730..8509)
                     /locus_tag="ACG8NF_002121"
     CDS             complement(7730..8509)
                     /locus_tag="ACG8NF_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0472592.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8526..8996)
                     /locus_tag="ACG8NF_002122"
     CDS             complement(8526..8996)
                     /locus_tag="ACG8NF_002122"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0472593.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9008..9427)
                     /locus_tag="ACG8NF_002123"
     CDS             complement(9008..9427)
                     /locus_tag="ACG8NF_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0472594.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9508..12285)
                     /locus_tag="ACG8NF_002124"
     CDS             complement(9508..12285)
                     /locus_tag="ACG8NF_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0472595.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12476..12700
                     /locus_tag="ACG8NF_002125"
     CDS             12476..12700
                     /locus_tag="ACG8NF_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472596.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3883 a         2751 c         2141 g         4418 t
ORIGIN      
        1 aataatgata acaaatgtac tataattact ttgtaaagag ttttggggaa gactctttca
       61 tcaacgtgta ttaagagaca agaaagaatc catatggatt ctttcttgtc tctttttcca
      121 ctacttttta tttttctgac actacatatt cttataactc atttctcata actgtgaaac
      181 aacttataaa aataacaaac tttaaacaaa acaagcttaa aatcaaagat gatccatctt
      241 aaaataacta aaacaaaaaa agtagtcgtt gttaatcctc attaataatc gttttctttt
      301 tttcaaacgc tccattttta tgtggaacat tattttttta aggaactttg tcaacatgtg
      361 gataactatt ttccacttgt gtcctggact agctgttttg ctgtaaaata caaaacttgc
      421 tgcagcaaaa agatcgttgc aagagaggct gttccgcaga tcgctggatc tagatttggc
      481 aaagtcaaga ctgcatcatg taaattggcg tggttgtcca aaaccaaatc agcaaattca
      541 aataatcgca aaccactgct atgttgcgat ttgatacttc gtgacaaatc aaatccagta
      601 acaatgatta atggatgacc attttctttg atccactgag ctagttcgat gattgccggg
      661 tttcttcctt cattcgaaag aagaagaaaa atgtcttctg atttgacaga aagctgatca
      721 ataattgcct gtcctgcacc ttctagcgtt tcatagacac ctccggctgg attttcaatc
      781 aatatacttg gtacaagatc cggggtttga tcccaaaatt caaatgctac accacttagt
      841 gtgcggctag catatatctg caatctacca ccttgattaa tttgtttcgc gatccaagtg
      901 ccagcttgaa gcatcagcgg ctcttctatc cttaatttct cttccaattg gtgataatag
      961 tcaaaataac ataaactcat ttatctctcc cgcctagcta tacttgacat gaatagaaac
     1021 agctgcctaa tctaaggaag tacataaaaa aacagtagtt agacagtaac acatgtggtc
     1081 tttgctgttt caaacacaaa aaaggcattg aacgaatacg cccaatacct tgacatagca
     1141 aataaaacaa cgtgcagacc atggaaaata tctacatagg ggtagaattt tcaccctaga
     1201 ttaggtacaa taaattaggt cgatgcactg cattgactat ggcaaaatga tcggtcaggg
     1261 tgtatgtgtg tgactggtca ttccctgatt cttagcgcca actaagggtc agggagcctt
     1321 tttttattca gctgtttcta tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc
     1381 aagaaaatac aaaaaaacat tggtatgatt tatatatata taaataacat aaaaaaagga
     1441 atggcttatc ccgatacaac aagcattcct ttaaaaaaga ttcttttata taagtgaaaa
     1501 aagctttttt ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc
     1561 tttttttgcg tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga
     1621 gagaatctgt actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc
     1681 ttttcatata tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc
     1741 aagcttccca actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg
     1801 cataccagat aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata
     1861 gactaataag caatagtggc aacacaaata tccaattgct tccttccatc agccacttga
     1921 gtatatcaag cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt
     1981 ttttataatt aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat
     2041 ttctcttatg taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa
     2101 tattttctga atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg
     2161 atctagagtc aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa
     2221 taacaaatag cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag
     2281 ctttttactc aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag
     2341 tccatattca atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc
     2401 tttaaactct ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag
     2461 ctgtatctcg tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt
     2521 cagtagataa cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc
     2581 tacaaaccct tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc
     2641 gtatttagat gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc
     2701 ttcataatcc atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc
     2761 cagcatgacg acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc
     2821 cagtcctctt ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac
     2881 taaagcgagt aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc
     2941 atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat
     3001 tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag
     3061 catttctttt gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg
     3121 aatgactgct tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc
     3181 atctatttaa cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt
     3241 tgctgctttt ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg
     3301 catctcaaca cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc
     3361 aacgaccatt cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt
     3421 accggcacgc caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc
     3481 actcaaaccg ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc
     3541 tgccaatcct cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat
     3601 agcgccggca ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac
     3661 atcattattt gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc
     3721 tgctttttga tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc
     3781 agcatggcta ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc
     3841 agccaaaact tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat
     3901 aatgattggc ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt
     3961 tgttggaaca acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata
     4021 tccgataatc gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt
     4081 attgatttca tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc
     4141 tgccaatgtt tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg
     4201 tttcatggat attcctccaa ataaataatt gataatttcc attatagtat acatttctta
     4261 aagttgggac taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact
     4321 tgaaataatc aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt
     4381 gcttgttcct aagaggaagc agttttgaca atgttactac cagcattact attaggaggt
     4441 taggaaactt ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc
     4501 taaatctgtc aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt
     4561 ttgttttagt caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact
     4621 tggactttta ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg
     4681 ttggcaccca catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat
     4741 gtgttttata acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg
     4801 ctctctttat tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag
     4861 tagcggcgta tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt
     4921 gtcactacat gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct
     4981 tctactggat agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt
     5041 ttagccacac gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg
     5101 gttgtttctt ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc
     5161 gatggaagcc agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat
     5221 tgattccctg ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat
     5281 aagccaaatg catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg
     5341 agctgttcat agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct
     5401 gctgccataa aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg
     5461 catagctgag acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact
     5521 ttcttgattt gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa
     5581 ataacgatag aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc
     5641 tgtacaaaga caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa
     5701 gcatacgttc caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta
     5761 tcgatgattt cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt
     5821 tcttcttcat tgaatttcag catgatttcc acctcttata atttactttt caagactgta
     5881 tcaaagtctt caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa
     5941 gcgaatagtt gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt
     6001 gctaatccaa gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca
     6061 acgtaccgac tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct
     6121 tcaataaaga atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct
     6181 tctaactcaa atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct
     6241 ttagctgttc cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat
     6301 aaactttgat atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg
     6361 attattttgc gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta
     6421 cttattccaa ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt
     6481 gtaacgaagc ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa
     6541 gcaagtggac tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc
     6601 atttttactg cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta
     6661 tcgattgaag gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca
     6721 gtaagatcac cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa
     6781 cccgttgcta agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca
     6841 ttctttttca agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta
     6901 aacattttct ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa
     6961 agctagtccg atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat
     7021 gccaaatgct ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt
     7081 cacagcattt tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt
     7141 cattgctcca acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat
     7201 accgtttgcc tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc
     7261 tagatagcga aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa
     7321 aatatttcct tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc
     7381 ccaaaagaaa gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc
     7441 gatcgcagtc acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat
     7501 tcctaaaatc agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa
     7561 agctgctgcc atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata
     7621 aacataagcc atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg
     7681 ccaaaatacg tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt
     7741 catcttcttc tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat
     7801 tgactaccac aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta
     7861 agtaagaagc tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca
     7921 aacgagcaag catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct
     7981 tttgaacgaa gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat
     8041 aagaaaccat taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca
     8101 tatgctcgac tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa
     8161 ttggaatcaa aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca
     8221 aacctagtaa aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta
     8281 aaatcccacc ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa
     8341 atgctaactc taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc
     8401 ctgtaaacag ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg
     8461 attgtgcgac aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc
     8521 ctttcttaca aaacttctct tacatccact tttttatctg ctggtacttg acggatttct
     8581 acttctattt gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt
     8641 accgcttttc cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt
     8701 ttgatttctg gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg
     8761 aataacttat atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat
     8821 ttgatcacta gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca
     8881 ttcgtaggta catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat
     8941 gaaaaagcta cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt
     9001 cttcctccta aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga
     9061 tcattttttt tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt
     9121 ccaaccgaga tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat
     9181 gatcccgatt gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca
     9241 gaacaatgat ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat
     9301 caaaattctc atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca
     9361 gctctaatga atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc
     9421 tatccacttt ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca
     9481 aaattagtgg caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt
     9541 atcttcactc aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata
     9601 atgctcttca atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg
     9661 cttccatttc tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat
     9721 gagacagctc acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa
     9781 ctgcacctct aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag
     9841 aatagtcacg gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa
     9901 tatttccttc tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt
     9961 ttcccccgag atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt
    10021 ggtgccaatc attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc
    10081 ttttaattgc tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt
    10141 ttctagcagt tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag
    10201 tgctttgggt ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac
    10261 agtctcttct gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat
    10321 tgctaaaggt gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa
    10381 aaactgatgg atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac
    10441 atcgctgttt tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag
    10501 tggcatatca aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat
    10561 actgctagca gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt
    10621 tgaccatttc gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc
    10681 aatatctaat ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg
    10741 ataagactga gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc
    10801 cggtaaccat ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt
    10861 tccatcgaat ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg
    10921 tgtccaataa accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc
    10981 aaataattga atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt
    11041 tttcttgaaa tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg
    11101 ttgcttctcc attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc
    11161 tgtattttta gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg
    11221 gatttcaggc tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt
    11281 tttcagctct cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc
    11341 actgactgac aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag
    11401 ttctagccgt tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat
    11461 tcttcgaatg aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag
    11521 acgtgttttg atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc
    11581 aagataggtg aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa
    11641 caagatccca ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta
    11701 cgctccctta acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg
    11761 tgcacaatta aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata
    11821 ctgatagacc aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag
    11881 tggtaatccg ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa
    11941 actggcgtcg tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg
    12001 ttcctctttg agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt
    12061 ttcaaaagct tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg
    12121 ctcatcattt aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc
    12181 agctaacctt tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg
    12241 gttctctaag taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata
    12301 gaaaacggat tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt
    12361 atagacagca gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc
    12421 agtttttata ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa
    12481 atcggataaa tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg
    12541 atcttatctc gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac
    12601 tcgttttatt ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat
    12661 cttgattttt gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt
    12721 cctgtctgtt tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat
    12781 aatgaggaaa aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc
    12841 tttttattga ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag
    12901 aaacattgat gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc
    12961 ttttgacttg atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa
    13021 accatagttt tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa
    13081 atatttttct cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact
    13141 tttttacaaa aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaa
//
LOCUS       DAZKOM010000081        13099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000081 DAZKOM010000000
VERSION     DAZKOM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.081"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8NF_002126"
     CDS             <1..608
                     /locus_tag="ACG8NF_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472597.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACG8NF_002127"
     CDS             628..1656
                     /locus_tag="ACG8NF_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0472598.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACG8NF_002128"
     CDS             1653..2411
                     /locus_tag="ACG8NF_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0472599.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACG8NF_002129"
     CDS             complement(2497..3237)
                     /locus_tag="ACG8NF_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0472600.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACG8NF_002130"
     CDS             3298..4551
                     /locus_tag="ACG8NF_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0472601.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACG8NF_002131"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACG8NF_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACG8NF_002132"
     CDS             5222..5548
                     /locus_tag="ACG8NF_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0472602.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACG8NF_002133"
     CDS             5616..6329
                     /locus_tag="ACG8NF_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0472603.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /gene="licT"
                     /locus_tag="ACG8NF_002134"
                     /pseudo
     CDS             6639..7491
                     /gene="licT"
                     /locus_tag="ACG8NF_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7612..9477
                     /locus_tag="ACG8NF_002135"
     CDS             7612..9477
                     /locus_tag="ACG8NF_002135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0472604.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9491..10934
                     /locus_tag="ACG8NF_002136"
                     /pseudo
     CDS             9491..10934
                     /locus_tag="ACG8NF_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11042..11743
                     /locus_tag="ACG8NF_002137"
     CDS             11042..11743
                     /locus_tag="ACG8NF_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0472605.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11869..12381
                     /locus_tag="ACG8NF_002138"
                     /pseudo
     CDS             11869..12381
                     /locus_tag="ACG8NF_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12779..12988)
                     /locus_tag="ACG8NF_002139"
     CDS             complement(12779..12988)
                     /locus_tag="ACG8NF_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0472606.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         4407 a         2191 c         2757 g         3744 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgat
//
LOCUS       DAZKOM010000082        12897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000082 DAZKOM010000000
VERSION     DAZKOM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.082"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1147)
                     /locus_tag="ACG8NF_002140"
     CDS             complement(<1..1147)
                     /locus_tag="ACG8NF_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0472607.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1269..1865)
                     /locus_tag="ACG8NF_002141"
     CDS             complement(1269..1865)
                     /locus_tag="ACG8NF_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0472608.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2065..4707)
                     /gene="alaS"
                     /locus_tag="ACG8NF_002142"
     CDS             complement(2065..4707)
                     /gene="alaS"
                     /locus_tag="ACG8NF_002142"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0472609.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5095..5526
                     /locus_tag="ACG8NF_002143"
     CDS             5095..5526
                     /locus_tag="ACG8NF_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472610.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5571..6920)
                     /locus_tag="ACG8NF_002144"
     CDS             complement(5571..6920)
                     /locus_tag="ACG8NF_002144"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0472611.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6990..8153)
                     /locus_tag="ACG8NF_002145"
     CDS             complement(6990..8153)
                     /locus_tag="ACG8NF_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0472612.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8282..8605
                     /locus_tag="ACG8NF_002146"
     CDS             8282..8605
                     /locus_tag="ACG8NF_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472613.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8788..10137)
                     /locus_tag="ACG8NF_002147"
     CDS             complement(8788..10137)
                     /locus_tag="ACG8NF_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0472614.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10206..11159)
                     /locus_tag="ACG8NF_002148"
     CDS             complement(10206..11159)
                     /locus_tag="ACG8NF_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0472615.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11261..12451)
                     /locus_tag="ACG8NF_002149"
     CDS             complement(11261..12451)
                     /locus_tag="ACG8NF_002149"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0472616.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(12651..>12897)
                     /locus_tag="ACG8NF_002150"
     CDS             complement(12651..>12897)
                     /locus_tag="ACG8NF_002150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472617.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         3710 a         2714 c         2227 g         4246 t
ORIGIN      
        1 aatataaaag ataattgtga tcaaacgtaa tttccaattg gtaatctccg attttttctt
       61 ctatttgact acgtaagatt ctttctagtt ctgtatcaac aaactggaat ttctcaggga
      121 ccactacatc aaattgcagc gtttttcctt gatgaattac tctaaaatca tgaatattaa
      181 gccctgtacc tgtttcaaga agaatttgct ttaactgccg caagatatta tagtattttt
      241 cgttatcaac aggcattgga tccaagtgac agacaagttc cacatccaaa gactttttaa
      301 agtctttttc tatcagatca ataatcttat gagccacatt caaattcatt gattcatcta
      361 cttcgatgtg gactgatgca aagcgtttat tgggtccata gttatgcacc aataaatcat
      421 gatagccaag tatatcatcg caagcattta aatgctcttc catttcttca atctctgctg
      481 gcgttgggcg acttcctaat agttcgtata caaaatttcg aagcatcgtc aatccactat
      541 aaaggatata taatgctaaa gcaaaaccta tatatccatc aatacgccat ccagttaccc
      601 attcgattgc tgctgaaagc aatacagcca gtgtagtaaa gacatcatta taactatctg
      661 ttgctgtagc tctcaatgtt tcagaatcaa tggattgcgc aataacctga tacattttgc
      721 cttgtgcaaa tttcaaaaag atggaaatga ttaggacaaa aaagacgatg ggtgttaaag
      781 agacattttc tggatggatg attttttcag cagaagactc taaaaattga aatcctacat
      841 aagtaatgat gatcgaaaca aataacccac tgatatattc gaagcgttcg tgtccataag
      901 gatgttcctg atctgctggc tttgcagcaa ttttaaatcc gatcaaagtc aatatggatg
      961 aagcagtatc tgatagactg ttcactgcat cagccataat ggagacactt cctgaaaaaa
     1021 gcccgatcat tagcttagag acaaacaaga tcagattcgt aaccaatcct aagatacccg
     1081 ctagtactcc tttttttgtc cgctgttgcg gtatttgttt ctttttttct tcttgtttct
     1141 tagccaagtt ttttacccct tctagtttgc ttgttgctca ggtaaaaaga tggagaggac
     1201 gattgttttc ctctcctctc ttcctttttc tatctatcgt gcgtcctatt actattaaaa
     1261 gatggtcatt attctggttt caacggtgtt gctgcaaata acgcttttgc tagtaatggt
     1321 gttatgattc ccgcacctat tgcttctggt atcccattga ttgctgcaac ccctgctaag
     1381 gttttaaata aaagagcaga gtctacacca taggcatctg ccgttgcacc tgtataaaat
     1441 aatcccatga atcctaacac taagactgta ttgaccaatg aacccagtac ccctgcaaag
     1501 gctgagctga tcgttattga tttatttttt tgataaatcc agtggaaaac caagcctgct
     1561 actaagccaa caagtactct gggaactacc gagataatcg gattcgtgaa cactaatgta
     1621 tcaaatggcg ttgttggcat ggcataggca cgaacaattg tagcgattcc ccaaaacaag
     1681 ccaatcacca tgccatcttt tgggcctaat actactgcag caataattac tgtgatatgg
     1741 ataattgtta aactaatgaa tcctaatgga ataaacccca gccaaggaac catcgcttgt
     1801 acgatgatga ttgctaacaa gatcgcacgt aagactagtc gaaaggtctt atttctacta
     1861 ttcatttttt cacccctttt tatttatact aatcgtaaaa atttctgtca ctttcgtcaa
     1921 ctaaagacca tccgaatccg accatttgat tagatgcgcc atccaattga aaaaagtatg
     1981 agggcaagac ttgtttgtct tcgcccccat aatcatgttt cgttcaatgt ttgaactttt
     2041 gtttttctgt caaacgtaaa cgccctattg ttgcgcaacc cattctttcg cagctttaag
     2101 tgcatctgga ataccagcag gatttttccc gccagcttgt gccatgtctg gacgtccacc
     2161 accgccaccg cctactttcg gtgcgattgc tttaatcaag tcgccagctt ttagtccagc
     2221 ttcatttgat tctttcgtca ttgccgctag cagactgact tttccttctt gttcgtttgc
     2281 taaaacaaga atatctgaaa tatttttttg tttccattgg tcagctaatt gacgcagctg
     2341 attcatatct ttcactttta cttgtgctgc aatgattgtt tttccattga tcgtttctgg
     2401 atctttgaat acatcaccag cttgttgatt cgctaacttg ctagctaatt gttcattttc
     2461 cttttgtagc tctcttactt gctgttgcaa ttgttccgct tttgaaacaa cttctttcaa
     2521 ttgaggtgat ttaacaattg ttgctacttg ttttagttgt ttttcttctt cttcaagtag
     2581 ttcgtacgct tctttacttg ttacagcctc gattcgtcgc acgcctgcac caattcctga
     2641 ttcagaaacg attttgaaga ttccgatgtt ttctgtattt gctacatgtg tgcctccgca
     2701 cagttcgatt gaccagccgc caatatttac tactcgaacg tcatgtccgt atttttcacc
     2761 aaacaaagcc attgcaccca tgtttttggc tgtatcgata tctgtttcaa ttgtttcaac
     2821 tgggatagct tcccagattt tttcgttgac gattctttcc atttcagcta attcatctga
     2881 agtgatttga ccaaagtggg taaagtcgaa tcgtaaatgt ccaggagcga ctaaagaacc
     2941 agcttgattt gcatgctcgc caagtacttc tttcaatgcc ttgtgcagta gatgggttgc
     3001 tgtatggttt ttgatgatcc gattgcgcat cgattgatcg atcaatagat gataggtttg
     3061 tccttcactc attggtgctt ttacttctac ggtgtgcata aattgaccat taggcgcttt
     3121 ttgaacatct gtaacaactg ctacgatttc gcctacttca ttcatgatcc atccttgatc
     3181 cgctacttgc ccacccattt ctgcatagaa aggtgtttga tcaaagatca tttgagcttc
     3241 accttctgcg acttcattca aaagagactc acctttaacg atgaccaata attgactatc
     3301 tgattcttgt tctgtgtatc caatgaattt actttctact ttgatatctg tcaataaagc
     3361 agattgtacc cccattgatt tttcagtact gcgggcagag cgagcacgtt cgcgttgtgc
     3421 atccatttct ttttcaaaac cagcatgatc aactttcagt ccttcatctt ctgccacctc
     3481 ttccgttaat tctaccggga agccatatgt gtcgtatagt ttgaagatgt cttttcctgc
     3541 tagagtcgtc ccgttttcgt tttttacttc ttggatcact tgatttaaga tttccaaacc
     3601 ttcattgatt gtttcattga aacgttcttc ttctgtacgg ataacttttt caataaattc
     3661 tttttgttga agtacttcag gataataact taccatgatt tccccaacaa cagggactaa
     3721 acgatataag aatgcttgtt caatacctaa ttttttccca tgcatgacag cacgacgaag
     3781 caaacggcgt aagacatacc ctctcccttc attcgatggc aatgcaccgt cgccaatagc
     3841 aaatgaaagg gcgcgaatat gatctgcgat tactttgtaa gaaatatctg ttactgggtt
     3901 ttcaccatat tttacttgtt tgcttagctt ttctacggcg tggataatag gcataaacaa
     3961 gtctgtctca aagttcgttg acgcatcttg aacgattgag accatgcgtt ccaatcccat
     4021 ccccgtatca atgtttttat gaggaagtgg ttcaaatgtg ttatcaggct tatggttgaa
     4081 ttctgaaaat actaagttcc agatttctaa ataacgttca ttttctcctc ctggatagtt
     4141 ttcaggatcg tcttctgcta aatcgttata agcttctcca cggtcataga aaatttccgt
     4201 atccggacca catgggcctg caccgatatc ccagaagttg tcttctacat caataatgtg
     4261 atctagtggc aggccaactt cttcatgcca gatgcgtttt gcttctgtat cttttggata
     4321 aactgttaca taaagttttt ctggatcgaa tgccatccat tttggatcag tcaaaaattc
     4381 ccatgcccag tggatggctt catttttgaa atagtcacca atcgaaaaat tacccagcat
     4441 ctcaaacatc gtatggtgcc gtgctgtttt accgacattt tcgatatcat ttgttcgaat
     4501 agatttttgg gcatttgtga ttcttggatt ttctggcaca actgaaccat caaaatattt
     4561 tttcaatgtt gcaacaccag agttgatcca caataaggtt ggatcgttca cggggatcaa
     4621 agacgcacta ggttcaatcg tatgtccttt agattggaag aaatcaagaa acatttgtcg
     4681 aacttgtgca ctacttaatt gtttcatgtc attacctgct ttctataaat taaatacaaa
     4741 aaaacaccat cacagccaag gacgaaaact cgcggtacca ccttgattgc aatgatgtgt
     4801 aatcattacc tcttaaactc gataacgctg agctgcgtcg gtatttctac caaaatagaa
     4861 aaagggagca atttgattag tgactattct tttttcagcc aagaaagaat tttctgtgag
     4921 tcttaaaaaa caaatcatat ccttttcgta cttaattagt atacgcattc tattgatcac
     4981 tgtcaatatc tcaattttta ttttcatttc cttttggtca tactccacga tttttggtac
     5041 gataagaata aaggaatcaa tacatctctg ctattcatga caaggaggaa tatgatgaaa
     5101 aaaatagttg gagcggatga atggatacgc gcagctagct gctttttgag atatcaagtt
     5161 tttgtattag aacaagggat tcctgcagaa ctagaattcg acggaaaaga tatcccgcaa
     5221 caaacctatt atctatttct tgatcaaaaa agaccagtag ctactttgcg ttatgaaaag
     5281 aaaacaccta cttgcttcaa gccggaacga ttttgcgtag caaaagaata ccgaggaaaa
     5341 gggttaggcg gtcagttgct tgaagctgct gaaaaacggg cggtttcaga aggttgtcag
     5401 actagttatg tagtagctga aatgacggct gtagattttt atgaaaaaca agggtatcat
     5461 acttgcacag aaccttttat tgaagatgaa gtactttgta tcggaatgaa aaaaagtcta
     5521 gcttaaaaac aagagattgt gacacgcctt ttgtcacaac ctcttgtttt ttatttatgt
     5581 gaatttttct tatttttacg agcagtacgg ctttgctgtc tgcgttcgat tttgcgttct
     5641 tttttatttt tttctgcaat tgcccaatcg atttttttct tataaccagg cttgatcttt
     5701 ttctttttct tcttcacaag accaatcagc gtgggatcta atacatcttt tgatttttct
     5761 cgtttggcac gtctattacg atcgtatgtt ttgacgattt ccccgttttt gatttctttt
     5821 ggttcaaatg agacgcccaa tttttctatc gcatcaatgg cttgctcatc atttggcgaa
     5881 tataacgtaa tggccgttcc ttttaagcca ttacgtccag tacgtccgac acgatggata
     5941 aagaagtcta gatcttctgg aatttctgca ttaataacat gagagacacc ttcgatatcg
     6001 atccctctag cagctaaatc agtcgctaca acatattgat actccagatt ctgtacttgt
     6061 cgcatcactc gttttctctc tcttggggtg atgtctccat gaatctttgc tactttcaag
     6121 ccttggtttt tcaagtaatc ggcaatttca tccacatgct gttttgtatt ggcaaatacg
     6181 attgctaaat aaggatgacc aattgtcagc agttgataaa tcatttgatt ctcatcttta
     6241 ccttttgttg aaatcagcca attttcaatg tcttcagaaa taactgcttt tggtttgatt
     6301 tcttcaatga ttggattttc catgtatttt cgtaagaatg gacgaagttt ttctggaata
     6361 gtggcagaaa aaactaaaaa ctgcagcttt tctggtaatc gacttgcaat ttggtctact
     6421 tcatttaaaa aacccatatc caatgtcata tccgcttcat cgacaacaaa tgcaaatgcg
     6481 gtgtgaatct tcagcgcttg ttcattcatc atgtctaata ttctaccagg cgtgcctact
     6541 acaacctgtg gctgttggtg atgcagacga tttaattgac gctgtttgtc tgtaccgccg
     6601 acaaaattcg agacacggat ctccggtgta gaaaatttag cgatttgtgt agctgcttca
     6661 tagatctgtg cagctagttc cctacttgga gctgtaataa caacttgtac ttcctctttt
     6721 tctgcattga ttttttcaaa caaaggcaac aaaaacgtat gtgtttttcc tgtaccagtt
     6781 tgagattgcc caatgatatt ttttcctttt gcaattaaag gaatcaaacg ttcctggacc
     6841 tctgtgggct gtttgaagcc ctttgcggtt aacgcttcat tgataaacgg tttgaagcgg
     6901 aatatatcaa aattattcat tcgtgcacct cttctcttta aattatcacg actttggcat
     6961 tataacacag acaaagcgtt tttgctcagc taatttcgag aatcatcatc ctttgtttgg
     7021 ccaatcgtgc taacagtata tgtgtgacta gctttttcat aaaacagaat ccgttttttt
     7081 ctcaaaaatg atagcaagat atttaaaata aaaatacctt gtagtaacaa gacaccgaga
     7141 gaaatgaata ctgcactcat ctgcaacacc gtattcgttg tcgaattcat gatttgctga
     7201 agcaactgcc ccaaccagct taaaccataa aaaagaaagt acagataccc gtagcggatc
     7261 atcaatgctt ttaaagaaat tcgttcgcgg tcttcttcca ccaaacggat acgcacaatt
     7321 tttttgccaa gtgtccatcc attggttata aacggtacaa tcataaagta tacccctact
     7381 tctaataaaa gccaccagat atgagaagta actgcccctg aatcgaacgc aattttaaag
     7441 aaaatcgccg ctaccaaatt aataagagat aaaaatgtcc agtcgatcaa ataagcgacc
     7501 aagcgtcgag tcaatgagac agtctttcct ttttcataag aaagttcatc cattcgtctt
     7561 cttgtaggaa gcataaatgt aaataacggc gtaacgacaa aaccaatgat tccacccaat
     7621 gtatttgtca ctaaatcatt cacatcaaat aagcggtatg gtcttgggta gataaaataa
     7681 agtccactta gctgcgtcaa ttcaaaaaat agagaaaggc agaaactagc aatcatcgtt
     7741 ttcataaaag aaagccggaa ataatatctt aaataaatgc caaacggtat gagtaataaa
     7801 acattaaaag ctggttccaa aaatacactc tgcttcattg ccggcaggta ggtactaggt
     7861 tcagacaaga ccaataccgt gttgtctctg aaatgttgcc atgaagcaaa taaatgcagc
     7921 tccatctttg gccctgtcat ttgggcaacc gtctcctttg ccggaagtgg taagatcacc
     7981 aaaaaatacg cacacaataa ataaaaaacg aaagaataca aaataactgc ccttgttact
     8041 agaaatgttc catactttcg atattcataa ataaaaaaaa cagatgagat cgctaaggct
     8101 aaaaatggaa aaacaatcag cgcaaaacga atcggttcga tataacttgc catatgactc
     8161 ctccttgatt ctttgtatct ctcttatctt agcagtcctt tgctgtttca agcaagtaac
     8221 gaggctatta tttctcattt ctttcaaagt aaagtaagat aaacacaaag ggggtgtcga
     8281 gatgctgaat atgtactttg tttttggcgt acctattttt ttactgttcc tttatgctac
     8341 gatcgcttat gtaagaaaaa gaactacaat tcattattta ggatttattc tcctgattat
     8401 ttcgggtttc atgctagtct tcaatcttca gacttggcag caagcacttc ttgaaatgga
     8461 taaaatgact ccacatgctt tgtctaaagt gcttggctac cctgtctatc tgatttggct
     8521 tcccattttc atttctggtt gcttggtttt attaaatatt tatcgcggcg tacgccgaat
     8581 agttcagtta cgcaaatcaa agtaaaagac agaaatcggt tgagcccgat ttctgtcttt
     8641 tactttgcta tttcatcgta gacaaaacga catttacgtg atcaaaatta acgagtactt
     8701 ttgacatttc ttccacgtac tcctcattta aataaaatat tttgtttggc agagaagtgc
     8761 agttctaata aaattcgaat gttttagcta tctacgtttt aaatagagat aaatcgggat
     8821 accagcaagt gtcaatacta tccccatcga agctaaaaag gtctgagtga taagcgtgct
     8881 tgatacgata aaagtccctc caatgattgc tactagtgga ataaacggat acattggaac
     8941 tttgtatggt cgaaacaaat caggttcttt tttacgaaga agaatgacac caacaaagac
     9001 catcgtataa aaaatccaaa tgacaaagat cagcatatcc gttagtgtat caaaaccacc
     9061 gatcatcatc atgccaatag cgatgactag ctctaagata cctgcggcta caggaacttt
     9121 agttttatca tgaagtttag caaagagctt gctaaaaggc aggttgtttt cttttgccat
     9181 aacataaggg agacgcattc cagtaagggt atagccattg atggttccat aaacagaaat
     9241 caagatacca actgtgacca gtcttccacc gaagccgcca aaaatcattt ttgcaacgtc
     9301 acttgccgca ttgctgtttc ctgcgactcc gtcgatagat gcggttctta agaacaccgc
     9361 atttactaat aaataaacga tcatgattcc gatgattcct aaagaaattg cttttggcaa
     9421 atctttcgcc ggctttttca attctcccga aatatttcct acgtgtatcc aaccgtcgta
     9481 tgcgaacatc gttgctagca agcccgctcc caaggctgag aaaaatgaaa gattttctcc
     9541 tgcttggatc ggaaacagtt ggaagtctac tccttcttgt cggaacaaac cgaaaatgac
     9601 gatcacaaac aaaggaataa gcttgcagac aagcgtaatc gactgaaaag cccctccagc
     9661 tttagaacct aaaaaattga tcaacaggat cgatacagca gctgtaacag ctacgggaac
     9721 aatcattgac tgggaaagac caaatagatt gacgaactgt gtaccgaaaa tgatcgacaa
     9781 ggctgctaca tttgctggaa aataaatcac gacttgcgcc cagcccaata aaaaagctgc
     9841 tgtatttccg taaatccgtt cgatatattt aatcatccct cctgtttctg gaatagcagc
     9901 tgccagttca gctcctgtta gtcctgcaca gacagaaatc ataccaccga gaaaccaaga
     9961 aaacatatgc aaactggcgg atcctgtaac ttctgctacg cttgcggctt taaaaaatac
    10021 ccctgcaccg atcactgtgc ccataacagt cgataaagca gtaaaaaatc ccatcgtacg
    10081 ttttaattca ttttctattt ttacttcttt tactggcgtg gcttgatatt cttccatccc
    10141 ttgtcccctt tcataccata gccaatccca aaagtataaa aaactttttg gatgtggttc
    10201 ttgttttata ccgtctctac caatttttct ttcagttttt ccaacatatc ctttgtcatc
    10261 acatctaagt cgtatttcgg cgaaaagccc cattcctctt gcgcacaaga aacatctagt
    10321 gaatttggcc aagattctgc tattgactga cgtacaggat ccacatcata atccatttca
    10381 aaatctggta tgattttttg aatagatgct ttgatctctt ccgggtcaaa agacatcgcc
    10441 gatatattga aggcatttcg atgaaccaat cgatcaggat ctgcattcat gagttttata
    10501 atggcatcga tcgcatctgg catatacatc atatccattg atgtaccttg atcaatgaaa
    10561 ctagtgtact ttccttcttt gattgctgaa taataaatat cgactgcgta gtctgtcgta
    10621 ccgcctcctg gcaaggttgc ataagaaatc aatccaggga aacggacacc acgagtatct
    10681 acgccatatt tcacataata ataatcacat agtagttctc cagctacctt cgttacccca
    10741 tacatggttg taggacgctg aagagtatct tgtggggtat gatcttttgg agtatttgga
    10801 ccaaacgcgc cgattgaact tggcgtaaaa aatttcagct ggaattctct agcgacttcc
    10861 aatgcattga ccagcccagt cacgttcaaa ttccaggcta attgtggttt tgcttctgca
    10921 acagctgaca aaagagcagc caaatgaatc aatgtgtcca cttgatattg ttctgctatg
    10981 ctgcgcattt tttcgtaatc cattacatct aggatctcaa agatatcgtt tttctcaatg
    11041 acttcaggca tgcgtatatc tgtcgcaatg acattttcac tgcctagttc ttgacgtagc
    11101 cgtgtgatca actctgaacc aatttgtcct aaacatcctg tcaccaatac ttttttcatg
    11161 atgatcctcc ctgatctttt ttactactat cttcagatga ttaacaatat atgcgtgaac
    11221 atgttctcta attttaaata gacgaaaaaa ctaaatttag ttaaataatt tccatttctt
    11281 ttcctatttt ttgataaact gctactgctt catcaagcat ttccctcgta tgttccgcag
    11341 tcggcatatt tctgatcctg cctgtcccaa gaggcacagt tggataaacg attggtttag
    11401 cataaatccc atgttcgatc agcttcttag agaaagtctg agtcactttt tcgtctccca
    11461 aaatgactgg agtaataggt gtttcagaca taccgatgtt atagcctact tttttcaatt
    11521 cttctttaaa ataattagca ttttcccaaa ctttttcgac aagttccgga tgttcttgca
    11581 tcaaagtaat cgaagccaat gcagctgcag ctgcacctgg agttagcgaa gtagaaaata
    11641 ggaatggccg acttctagct ttcaaccagt cgattagtgt tttcgatcca gctacataac
    11701 ccccaacaac acctatagct ttcgataacg tgcccatttg catatcgatt ttgtcgctta
    11761 atccaaaatg cttgacggtt cctgcacctt ttccagttac gcctgagccg tgcgcatcat
    11821 cgacgtaggt gatcagtcca tatttttcaa caatcggaat aatttccggc aatcgtgcaa
    11881 tgtctccatc catggagaaa acaccatctg tgatatacat gattttttta tatttcttac
    11941 tttctatcgc ttcctttgct tttttctcaa ggtctttcat atcttgatgt ttaattcgga
    12001 tgatttttgc tccggataag cggcaaccgt caataataga tgcatggttt aattcatccg
    12061 ataagatagc atcttctttt gtcattactg cagagatagc gcccatattg cagttgaatc
    12121 cagattgaaa ggcgattgct gcttctgtcc ctttgaattc tgcgattttt tcttctaatt
    12181 gctgatggat tttcagcgaa ccattgatcg ttcggactgc tcctgctcct acaccataag
    12241 tttcggttgc ttcaatacat gcttttttca attcttcgcg attggcaaat ccaagataat
    12301 tgttggatgc tagattgatc atcttttttc cgtctacgat gatacaagca ccattttcac
    12361 tttctagtat gtcgatcgta ttgtataacc ccttttcttt cagttgatcc aattctttct
    12421 ctaaatacat tgttagttct ttggaagaca ttgtctccac ctccatattc atctgtcttg
    12481 cagaacatac caaacatata tcgtgtcatt catctacaaa gcaagtatgc tatatttttg
    12541 ataacgctta cactgattat gagaattatt taaccacatt ttttatttac agtatctttt
    12601 tactttcaaa tgacttatat agtatgaaca attacctttt agataatgat ctattttact
    12661 ctacctgggc agataaatac aacacaacca ttgatagaag ccctttcgtt aaaatcaatt
    12721 gtattctgaa tatttaattt atttattgca tagaccaata aaaatacata aaatttccat
    12781 cttcgtttat cagcaatttt taattttact ctctgaatat ttcttaaaag ttttacttgt
    12841 tgaattcgaa aaaaggactg tgacaaaagt cttgtcacag tcctattctt ccgaata
//
LOCUS       DAZKOM010000083        12849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000083 DAZKOM010000000
VERSION     DAZKOM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.083"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1666
                     /locus_tag="ACG8NF_002151"
     CDS             29..1666
                     /locus_tag="ACG8NF_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472618.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1811..2551)
                     /gene="truA"
                     /locus_tag="ACG8NF_002152"
     CDS             complement(1811..2551)
                     /gene="truA"
                     /locus_tag="ACG8NF_002152"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0472619.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2742..3377)
                     /locus_tag="ACG8NF_002153"
     CDS             complement(2742..3377)
                     /locus_tag="ACG8NF_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0472620.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3524..4744)
                     /gene="sstT"
                     /locus_tag="ACG8NF_002154"
     CDS             complement(3524..4744)
                     /gene="sstT"
                     /locus_tag="ACG8NF_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0472621.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4963..5961
                     /locus_tag="ACG8NF_002155"
     CDS             4963..5961
                     /locus_tag="ACG8NF_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472622.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6157..7992
                     /locus_tag="ACG8NF_002156"
     CDS             6157..7992
                     /locus_tag="ACG8NF_002156"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0472623.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8052..8768)
                     /locus_tag="ACG8NF_002157"
     CDS             complement(8052..8768)
                     /locus_tag="ACG8NF_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0472624.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8995..9537)
                     /locus_tag="ACG8NF_002158"
     CDS             complement(8995..9537)
                     /locus_tag="ACG8NF_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0472625.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9542..9769)
                     /locus_tag="ACG8NF_002159"
     CDS             complement(9542..9769)
                     /locus_tag="ACG8NF_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0472626.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9848..11728)
                     /locus_tag="ACG8NF_002160"
     CDS             complement(9848..11728)
                     /locus_tag="ACG8NF_002160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0472627.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11917..12531)
                     /locus_tag="ACG8NF_002161"
     CDS             complement(11917..12531)
                     /locus_tag="ACG8NF_002161"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472628.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12673..>12849
                     /locus_tag="ACG8NF_002162"
     CDS             12673..>12849
                     /locus_tag="ACG8NF_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0472629.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         4005 a         2510 c         2038 g         4296 t
ORIGIN      
        1 ttttattact tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat
       61 catttttatg ttatttctaa gttttgctta cgtcatccct ctattcatgt ctaagggaat
      121 ctatcatagt gtcaatcagg atacttactt tcatttatct cgaatcatag gactagataa
      181 tgtatggagt tctccagtaa acttcaataa cttcgatcat catggaacaa tgatgaatat
      241 tttttatcca tggcttactc tttatccagc ttttttattt tataaaatga tgggaaatct
      301 tgttttaagt tataatatct actatttttt tatcactttt ttaacaatgg tcgtttctta
      361 tttttctatg aaacaaataa aaaacaatcg ttatatttcg ctgttatttt cgattattta
      421 tacattttcc gcctatcgtg ccattgacat attccatcgt gcttcattgg gagaagctgt
      481 cgctttaaca tttttgcctt tgatactaat ggggtgttac gaaatttata ttagagatta
      541 ccaaaaatgg tactggttgt ctattggcat gacgctagta gtgtatactc atttactttc
      601 tgtcgctatg gtttctgtat ttattggagg aacgttgttt ctttcttttt atttttggga
      661 ccaaaaaatc gctagactct taagtctttt aaaagctacc gtcctcacct tttttttaag
      721 tgcgggattc ttaattcctt ttattcaaca atctagagca caagaattga aagtcccact
      781 gggaaaagaa ttaagtggta tggctcctag tgatatgctg acacatattt taaataataa
      841 ctataacaac tatactattg gattattctt atttctcgga ttgattggtg cttttatttt
      901 tataaaaaaa ttaactgctg atgatttatt tattttcttt cttggtgtat ttgttctttt
      961 ttgttcaacc aatttatttc cttggcaact ttttaaccat acaccagtta agagtctaca
     1021 atttgtctgg cggttaaatg gtttttcttc tttatttata gcctatacga tgagtatagt
     1081 gatctattat attttttcta ctaaaaataa ttcatggaaa ataatggtct ttacgtttct
     1141 tgcactaata ttgcatctct caggagtaag caattctatc catgctaaca aagattcatt
     1201 aacacttata gcacctgaag acgctgttgc tatagctgaa aattacaatc atactgatta
     1261 tgcaaacaaa gagtcaatct atcatcctga tatgattaat aatgatcaat atttactagg
     1321 taaccaggaa ataaatccaa caactcattt tatgagtaac aaacttacaa ttgaattaga
     1381 taattcgaat aataaaaata ctgtcttgac gacacctatt tatcgttata aagggcaagt
     1441 ggcttctatt aatggcaaac tagtacaaac aaaattatct aagtttggca caactgaatt
     1501 aactattcct ccaggtataa ataaagtcgt tattacttat cagtacacaa aactagcaat
     1561 tgcttcaaga tatttatcta tcgttacttt gattttattt ttactttatc gatttacttt
     1621 ttctaaacaa aaacaaccga gaagagaagt acaacattct cactagattt ttatttaaga
     1681 aagacttttt agctgtacac ataaacaggc taaaaagtct ttttttatat ttctttaatt
     1741 tgcttcatac attatttaac atcgatattt atactgtgaa acatgcgttt catccattat
     1801 caaagtattt ttatcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt
     1861 ctcctgcatg ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg
     1921 cacctgtgcc gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc
     1981 cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa
     2041 tcgtacgcac tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg
     2101 tacccactaa tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat
     2161 aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt
     2221 attttttgcc caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt
     2281 tgatgacaat atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa
     2341 tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc
     2401 ttcctgatcc aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt
     2461 ttccttgaat ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt
     2521 catattcgat tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg
     2581 gtcttctctc taccttttta agattgtagc acaaaatata gctccttgct tctctaagac
     2641 agaagctata caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg
     2701 gatgaagtat ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag
     2761 atactcaacc aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct
     2821 attttattca gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc
     2881 gcaaaaagca aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca
     2941 ttttttcgat agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa
     3001 cctgatccga tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata
     3061 ctgaacaaac ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact
     3121 agggtagcta acacacgaga tgtactaaca aatgcagcat acaatgcagg caatgacagg
     3181 atgaaaaaga tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa
     3241 acctctccaa atacactgat cacgctcttc atttgatcta attcaggata atacaatcct
     3301 aatacgtacg gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat
     3361 ttacgtaaaa aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt
     3421 ataaacgact ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc
     3481 attaaaaact cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc
     3541 catctaaaca atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc
     3601 ctattgcagt aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga
     3661 taacaccgat aataaatcca ataccgacga tttgcatcgc gacatcatta gagataccaa
     3721 acaaactaca tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac
     3781 aagcagctaa agcagacaaa atacataaaa ggactttcaa aacaaaaggt gcttcaatcc
     3841 ctaaagaatg tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat
     3901 tgatcgtagc acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt
     3961 cagccaacat catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa
     4021 gtgcgctttc tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa
     4081 acgtgaggaa tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta
     4141 aaagctgcag atactctgcc aatccagcga ttccagtcgt agcaattgat tgataaacca
     4201 atcctaaaat ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta
     4261 tagatatctg atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca
     4321 acaatacagc ccagaataag acgccaaggt aattcgcatc aataagagat tgaacaggat
     4381 tttgtgtgac attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt
     4441 cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca ataactgcta
     4501 caagtgcaga aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct
     4561 tattcccaat tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg
     4621 cgattgcctt caagcaactg acaaacaacg ttcccaatat tgaaataaat gtccatgtgg
     4681 ggaaaaagat accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg
     4741 acatatccaa aaacttctct aacaaatgac caccctttca caattttaac aatcattgta
     4801 tcattttatg caaagaaact aaaggtataa ttttctaaaa attctaacaa tacaaacaac
     4861 catttcttgc taattttgta ctttttctgt ctacacgttg ttttttgaga tattctaagt
     4921 taggatgtta ttaatgggca acttatcagg aaacgttttc ttatgttttt gtccaatttt
     4981 tttgtgaggt gtttattctt gaaaaaaata aagaaggaga gttatggctt cctgacaatc
     5041 tttgtcgctt gtataatgat ggttttacag cgtatattga tttcagcagt cactattcct
     5101 acatatgcaa caaaccctgg tcgattctac atctatacat ttttacaaac ttccgttgtt
     5161 attggtgcaa atttgatccc actttacatg ggttaccaat atcaaaaaat cgcccgttta
     5221 aaagttttac attatttaag tcgttttgcc tttatattcc taatagccac actagtttgc
     5281 aatatctttt tttacgtaca agctggcagc ttgaatattc gtgattattg gcttatccta
     5341 acacctatca gtcagaatta ctttacttat gctgtttctt gtactttgct tttttgtagt
     5401 attccttata cagttggatt tttaaataaa ttatcgaaaa caacaattaa atatctacta
     5461 ttatttttaa caattggatt agttggctgc tctactctat ttaataaaga cccgtttgca
     5521 ttacagaatg gacagagtat tttatggatt ttttatttat ttttaatggg ttatggtttg
     5581 aaagaatgga actggaaaga aaaggtccgc tataagcttc ctcaagtatt tatcagcttg
     5641 attgttttat tcggtttgat tattttgatg acacaaattt cactcatcat cagaggaaat
     5701 acagatacag cgctgaggtt ttctaaacca tattctctct tcgcaatgta ttatactttc
     5761 tcttcgtttt tatttttaga aatgctatct gataaattag gtataaaagg aagaggaaat
     5821 tttcttgctg tatttttgat tgtcactcaa gttatatgta atttcccttt agtttcttat
     5881 cgcgtatcta catttatgaa acgtgaattt ccaaactcag ggaaggcatg gctgataaat
     5941 atcgggcagt tttttatttg atatttatta gcagtgtccc tcctagttat ctttttattt
     6001 tatatacaaa aaatccgttt cattcatcgt ttcatcgaat acttcgcttt tgattcgatt
     6061 gaacaattaa ctcagcgttg gaaaaaaaga attcattggc tttttgttca taaaagttat
     6121 tttttagttg cagcattttt ttactgcttt accttcgtgc agattttcgt tttagaacca
     6181 aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa tatttactca gcgacaagca
     6241 cccatgatcc tgacaatcat cattattatg ggatttttct tcttactatt gttgatttcc
     6301 aatcgttttt ggtacgcctt gactgctacc ttgatcatta acttactgct cactatttcg
     6361 acagtaatca aaatggaaat gcgggaagag cctgtctttc cttcagattt aaaaatgctg
     6421 accggattgt cagaactatt atcgatggtt agcccagtct tattaatcgt gggcggattg
     6481 atccttctcc tattattgat tactagtatt attgtacaac gtagactaca aaaacagtat
     6541 tccttgaaga ttcattggaa acgtcgattt atcagtatcg ctgtattatt aggatgtttt
     6601 tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt taatttattt
     6661 aaagtaaaca agactttctt taaccagcaa ggcgccgtaa aagataacgg accagtcatt
     6721 caattcctaa acaatatcga cattaaaatc atggaagaac ctgctggcta ttcgaaagaa
     6781 aaaattaatg gaataatgaa aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga
     6841 agtgactggt taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct
     6901 gcacgtgtcc ctaatctgac agtacctaca gaccctattc cttacatcaa aagcattata
     6961 acagaaggaa caggaggatt gatgttatcc gtcggttatg gtggcggaac agctaacatg
     7021 gaatgggaag gactgacagg cttgagtatc agtaacctgt ctgcttcgtt agttacacct
     7081 tatacccagc tagtggagcg tcagaaaata agtccaaata ttaccgatct attcgatgaa
     7141 aaaatagcca tccatccttt tacagctgct ctttatcgca gaaaacaagt gtttgataaa
     7201 tttggttttg acaggttcta ttatttagac agtccggata aattaactta tacggataaa
     7261 atagaacaaa gcccacgcat atcggatgat tcagcataca aagaaacctt gaaagcttta
     7321 caaagcaata cggaaacgtc acaattcatc caattatcca ctatgcaaaa ccatatgcct
     7381 tatgatgatt actatagtga attgaactac actgctgaag gaactgctgt cattcctaat
     7441 agaagaaaag aattacaaac ttatatgcaa ggccttcact ataccgatac agcagtaaaa
     7501 aaattcatcc aagaaatcga caaaatcgat aaaccgatta cctttgtttt ttatggtgac
     7561 catttacccg caatctatag tgggaatgat tcgaaaaaat atggattaga acagcaccag
     7621 acagattatt tcatttataa caacaagtat agtcgtgaac aagcagctac gttaaataaa
     7681 gaagttgttg ctcctaataa ctttggtgct atggctcttg agcatgcgaa tatcaaagta
     7741 actccttact atgcgttgtt gacagaagta tctaatcatc tacctgcatt aacaattgat
     7801 cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg tctttgttac agaggatagt
     7861 caaattatcc aagaaaaaaa tttaactgag aaacaaaaag aaattctaga agattatcgt
     7921 ctgatacaat atgatcttgt tgcaggtaac cagtacagtg caacatgggc gacacaaaaa
     7981 ataaaagact aacaaataaa ctgagcaagg ggttgtgata aaagacatgt cacaacccct
     8041 tgtttatctc attaatttgc ttgtccataa acgatttgat taaagccatt tttcaattca
     8101 ttattcgctg tctcgttgaa gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc
     8161 accaatactt ttggctgatt ttgctgaaac ccgaatagat agacgtgctt tttcagatat
     8221 ggttctgtct ctgcatcgga atagacggct gtgatttgat agtctgcttg acctgtccca
     8281 ctttctgacc attctgcaga aacaggcact ccaccaatag ccatcttcca ctccccgttc
     8341 aaaatagctt gagggacttt aagaccatat aagtccacac tcatttgatt cgtataactt
     8401 ttgtattgtt gtcccattgt ttggccccat ttggtcataa atgcctctaa ttgtgcggat
     8461 ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc tttctgaaga ggaagatgaa
     8521 gcctctacag aagaacttga agtagatgtt tgagttgttt cagtcgtttt ggaagactct
     8581 gacgaagtag aggtatctgc tgtttctgta cttgctgcag ttgacttgct ctctgtgcta
     8641 gttgttgtag tagttgcaac tgttgctttc gctgtttctt ttgttgattc ttttttcgat
     8701 tcattcccgt tggtacaacc agctaacaaa aaagctagac caattgttac taaaatcctt
     8761 tttttcaatt aaaaaactcc tttcgattac tcccacttct ttattctatc aaataagcaa
     8821 tgtgatttta ttgatttttc gtattttctc atcaatcttt caaatcttct aaaaactgag
     8881 aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt ttaatcttcg
     8941 tgattttcca atttacctca cgctttatat ttgtatttga agaaacatag acttttagtc
     9001 atcatcgtct tcctcatcca aatcttgtcg gacagttttt ccttggagtc tttgtacttg
     9061 gtagatgatt tctttattga aacttaaaag ttcaacaagc aattgttgca agccatattt
     9121 ttcttctttt tcagctaatt tgattgcccg tgtgataaag acattttgga aatcaaaatc
     9181 ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat ttgttttgac catgttcgta
     9241 aatttttcca taacgcatga cttcttctaa agtagacgga acgatttcat tttcaatcac
     9301 taactcatga gagattttat tgaaccatct cgcttcttga tcagcatatt ctgaaaaaag
     9361 ggtacttaat tcttgcgaag atgtacccaa aacatacaat tgtgcttgga tcaatttcat
     9421 ttcttctaat ttaaaatttg ctaatacatg ccccaccatg gcgccagcag ttggtttatg
     9481 atggtcgata tctgcttgtt ttgtttcttg aacaaatttt tcttccacta tattcattga
     9541 tttacgcctc ttttactttt gctgattgga cttcataacc taatttttcc acaactgcag
     9601 ctaattgttc agggtggact tcatctggat caaagtttgc tttgattttg ctcgcattaa
     9661 acaaaacttt cgtattgctt accccaggct gttgagaaac cgcatgttcg attttttgca
     9721 tacagcttgg acaagctagt gtatctaatt gcataataat ttttttcatc gtagttcttc
     9781 cttccttttt cttgatagtc ttattctaga ttacagcttc cataaaaaaa ttgattctta
     9841 tcaagtttta cgatttttct tctttgcttt cgtgtccaaa atacaataaa cgcatcgcgt
     9901 taaagataac gatcaagata ctagcttcgt gaacgaacat cccactagcc atgtaaataa
     9961 acccggcaaa taagccgttg agtaaaaata agacgacacc aatggcaatc actacatttt
    10021 cacgggtatt taaaacagtt ttcttagcca agcggtaggc atgaaccaac gattcaaaat
    10081 tagattgcat caagacaacg tcactcgttt cgatagcaac atccgttcct gatcccatag
    10141 caatcccgat atcagccgca gccaaagagg gagaatcatt gattccatct ccaataaaag
    10201 caacgcgcaa tccttgttct tggtattttt tcacaaattg aaccttttcg tctggtaata
    10261 attcagcatg gacttcatca atgcctaaca tatcagctac ataatcagca gtcatctgat
    10321 tatcacctgt cagcatcact aactgttttg ctcctgcttg tttcaactta gctaattgtt
    10381 cagcgacttc tggtcgaatc acatctgaca cacctatgat ttgagtgatt tttttgtcga
    10441 ttgctacaat aatgaccgag ctgcctgttt gttgtaattg atgtaaatct tgtacttgct
    10501 ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt gttgcccgca caaactgtat
    10561 gaccatcaat ctcagctgtg attccttgtc ccttgattgt ctgattatct ttgactgcta
    10621 attgttgaaa atcaatgtct ttctcctgag cataatggat cacggcgcgc cccagaggat
    10681 gatcggataa cgtttctaat cgagccacta aagctaagct gtccccttca tcctctgtat
    10741 agttcttgat agtggataca gcagtagttc ctttggtcaa tgtaccagtt ttatcaaaga
    10801 caaaagtatc gattttggaa aaagtatcca ttgcttcacc gcctttgact aagatgcccc
    10861 gtttcgctcc attaccgatt cctgctacat tcgaaacagg cgcaccaatg actaaagcgc
    10921 caggacaacc aagaaccaaa acagtgattg ctaaacgaaa atcacgacta atcagaccaa
    10981 caaccaacgc aataagtaaa acggttggag tataatactg agaaaatcga tcaatgaatt
    11041 tttccgcatg ggatttggta tcttgtgctt cttctactaa ttcgataatt ttggcaaagg
    11101 tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta gccatctgct aaaatcgttc
    11161 cagagaagac ttcatctcca ctgattttga cgacttgtcg tgattcacca gtcactgcgg
    11221 cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc cacaggaata gctccaccag
    11281 tttttactaa gacgatatcg ccttcttcca cgtcatcgat ttccacttct tcagtcgttc
    11341 catcttcttg gacaacacta gcagtagatg gggccatctc ggtcaaagcc ttgattgact
    11401 cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac aagaatgtaa
    11461 cgatagctga ctccttgtat tctccgatca taaaggcacc aagcacagca atagtaacga
    11521 gtaaatcaat tgagataact ttgactttca ctgcctgata cgcatggatc ataataggta
    11581 tgaccgcaat aatagaagct atggctagag caatgttata gaacaagaca aaaccagtaa
    11641 attttccgat aaaaccaaaa ataatcaaca cagctgataa ggcggtgata tgatttcgat
    11701 gctcttggat ccacttttgt aatttcatct ttctccctct tttcctttca ttcttacgag
    11761 actaagtata gatacttatc catcataaaa aattgaccac tatcaagttt agtcaaaaag
    11821 gcggttttct tttggtagaa tacactacaa aaaatagtta gcaagaagac tcactaaaag
    11881 tgatcttgcc aactattgtc gtttctatcg aaattttcat tgaatgattt tcctgtacag
    11941 catacatcgg agatctattt ctttatctaa gatttttatc ttgctaattt tttcctttag
    12001 tctttcaaat ccacgtcgtt catagaattg atacgtacag ccactatctg taaaaaggaa
    12061 tatttctttt cctttctctc ttttttcaaa ttctgttagc aacttggtac cgatgccttt
    12121 tgttttcaaa tcaggatttg cagctaaaaa aatgatctct ccatctggcg aatgcttctt
    12181 taaatatgct gcaagcattt gttcatttgc tgtctcgtaa gctcctttac tttctttagc
    12241 aaatatattt tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa aagaacgatg
    12301 tttctttttt tctcctttta tctccgccag caaaacacct gccaactcgt cctctgcata
    12361 caaagcaaga atttgagttg ctcgattcaa ctccatatac aaaaaatatc ttccgtaaag
    12421 attcagcaga agttcatttt ccatatatct ctcaaagttc attccttcaa cagcaaaata
    12481 gatagctttt ttgtaatctt tcttttctaa gggtttaaaa gttagtttca tcatttcgcc
    12541 tcctcaactt caatgattgt gttatactta cattataata gacacagtgt ctactatcta
    12601 taatcaatca tctataagtt tgtcgattaa gcgacagctt tgctttgata tgtcggataa
    12661 ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa agctttggct
    12721 gaaacactat ccacattatt agtaaataaa aagattcaag caatcactat acgcgaacta
    12781 acagaaacgg cagatgtaca tcgttcgact ttttatacac attataagga tatctatgat
    12841 ctatatgat
//
LOCUS       DAZKOM010000084        12789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000084 DAZKOM010000000
VERSION     DAZKOM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.084"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1920
                     /locus_tag="ACG8NF_002163"
     CDS             226..1920
                     /locus_tag="ACG8NF_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0472630.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            2608..4485
                     /gene="ltrA"
                     /locus_tag="ACG8NF_002164"
     CDS             2608..4485
                     /gene="ltrA"
                     /locus_tag="ACG8NF_002164"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0472631.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4616..4993
                     /locus_tag="ACG8NF_002165"
                     /pseudo
     CDS             4616..4993
                     /locus_tag="ACG8NF_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            4993..6198
                     /locus_tag="ACG8NF_002166"
     CDS             4993..6198
                     /locus_tag="ACG8NF_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0472632.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6214..6858
                     /locus_tag="ACG8NF_002167"
     CDS             6214..6858
                     /locus_tag="ACG8NF_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472633.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6869..7675
                     /locus_tag="ACG8NF_002168"
     CDS             6869..7675
                     /locus_tag="ACG8NF_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472634.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7697..7927
                     /locus_tag="ACG8NF_002169"
     CDS             7697..7927
                     /locus_tag="ACG8NF_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472635.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8473..8748
                     /locus_tag="ACG8NF_002170"
     CDS             8473..8748
                     /locus_tag="ACG8NF_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472636.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            8790..9212
                     /locus_tag="ACG8NF_002171"
     CDS             8790..9212
                     /locus_tag="ACG8NF_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472637.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            9231..10823
                     /gene="mobP2"
                     /locus_tag="ACG8NF_002172"
     CDS             9231..10823
                     /gene="mobP2"
                     /locus_tag="ACG8NF_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0472638.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10835..11170
                     /locus_tag="ACG8NF_002173"
     CDS             10835..11170
                     /locus_tag="ACG8NF_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472639.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            11317..11511
                     /locus_tag="ACG8NF_002174"
     CDS             11317..11511
                     /locus_tag="ACG8NF_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472640.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            11543..>12789
                     /locus_tag="ACG8NF_002175"
     CDS             11543..>12789
                     /locus_tag="ACG8NF_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472641.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRA"
BASE COUNT         4885 a         1989 c         2688 g         3227 t
ORIGIN      
        1 ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa tgaacaatat gaatgatgag
       61 taggaatgga gaagagagaa tgaaatatcc gttgatatgg ttagaaaaag atgttccgaa
      121 ggaagagacc actagaaaat ttcctcacac tttaagcctg ggtaagttgg gaaaagggca
      181 atcttttaac caagtaaaga atcaaacaag aagaggtgac tgtacatgtt agaagcaatt
      241 aaaacagctt tttcaccaca aaatgatcaa aaagtacttg atccattaaa gcaactagga
      301 aatcgggaaa tagaaattgt aaaatccaaa gggtatgatt tagatttttt aaaacaagtg
      361 atgccacaag gtgggttaaa ttttgatgaa gaatatgtga cgtatggtaa tggttattca
      421 cgatgtgtga cagtttatca ctacacaaaa aatcctacgc ttttttggtt ggccaatctc
      481 atgaacaatc ctcatacagt ggccacactc gatgttagca cagatgaaaa gcaaaaagtg
      541 attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc gatcagataa tgaacgaaaa
      601 gcgactgacc gaaataaaag cgcggaagaa tacctggaac tagcggaata tgctagaaaa
      661 ttgacttcta atggggaaat ttctaagcaa gtcaaagtga gagtacatgt ttacggaaac
      721 acgattcaag aagtagatga acgagcgcag gaactgatcg aaaatctcaa agggatggat
      781 tatcgtgcgg tgacttatct gcataaaacc aaacgagatt ggcaatcgat gtttaccatg
      841 ttcgatgaac aagctgaatg gttcggaaat atcaaagggc aatcactacc atcgttaaac
      901 attggcggag gtttcccgtt ccaccaccaa acgctaaaag atccaggagg gacacaatat
      961 ggggaaacca tgacaggcgg cccatttgtc tggaatgcga cgtataaaaa tagtgtacgg
     1021 ttgtctacta ataccttgct tctaggaatg atgggagctg gtaaatcgac cgttcttaaa
     1081 atgatcgcgg aagcccattt agctgcagga gactttattt ggggctttga aaagggaaaa
     1141 gactttattc ctttcttaaa ggaatataac ggtattatgg tgcgtttaga cggatctgac
     1201 ggaatgatca atcctttaga gatatttgcc acacggacgt atgatgaagc atccagttta
     1261 tatgacgatg gatcagtcaa agatttgaag atcaatgaag cagcgtccta tcaaacacac
     1321 ttagacaaag tggtctatca ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa
     1381 gcagaattta agacctactt aaatcgtttt tatgaagagt atggaacagt accacgcggc
     1441 tttacttcct caaattcgcg ttcaaatact gatacgcaag tcacagggaa agatccagaa
     1501 gcttatccga cgttcaaaga atttttagac tttttaggac aactggaatt accaggtgct
     1561 tcccaagaga aaaagaatcg aaaagaagaa atggaatcca tcgtagaatc cttatgtgag
     1621 acgtatggca tgatttttga tggtcatagt acgattcgac accttgacca acaacagttg
     1681 gtcttttttg acattgactc tattacaggc ttagatcgag gggtacaaca atgtcaaatg
     1741 tacatggcta tgacgttgat ttggaatcag gcattgattc aaggaagaag acaacgacgt
     1801 ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac gcttcttggc gatgattgat
     1861 gagtgccata atggtgcgac ctgttctgca gtgaaaagct gtgggttgca agtagtctaa
     1921 aaactattgt aataactctt ataacgagaa gtcaaatgag tgggtaacgc cacaaagggc
     1981 ttccactgat actccgacat gcaacaagta gtttatgcag accaacgttg tgtgaagctc
     2041 ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata gtgaatacca cgaaagttct
     2101 ctagaggtaa agggtgtatc cgataagtcg ggtgtccaaa aaaacttaat atggtgagta
     2161 tgtacaaacg tactgacgaa ctagcgaatg tactgtccga aaggcatagc tcctagaaat
     2221 gggagagaca gtgagcaaaa aatcactcat tgtgctaact tgtggttgag taaaagtcgt
     2281 tgttatgaaa gaccatgctg cgttacaggc gtaggagaag ttagggggca aaagctagca
     2341 tctaagttaa ggcatgtgga tagatataag ggaacagggg aagccatcaa catggagagt
     2401 gcatactcga tgaagtggta gtaagaaata tgtgaatgac aaataactta ctttagtgat
     2461 ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg aactggagga acaggctcga
     2521 ttcgtctgta aaagtcatga aacccgttca accatgggat tctcgtatga ctaaaaaggt
     2581 cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa caagtccaaa agataggaag
     2641 ttgcggcatg cagaatatta tggcatgacg gaaatcttcg ataagatata ctccaaaagt
     2701 ttgaagggag ataatttcaa gaatctaatg cagattatca tttctgataa caacattgtc
     2761 ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga caagaggaga agatggagta
     2821 acgattaaag atattgaaaa tctcaaccgt aatgaattcg tagaaattgt taggaagcga
     2881 ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa ttcctaaata taatggtaaa
     2941 ttaagaccgt tgggtattcc atcaatatgg gatagaatcg cgcagcaatg catcttacaa
     3001 gtattagaac caatatgtga agcaaaattc aataatcaca gctatggttt ccgcccaaat
     3061 agatcagcag aacacgcaat agcagattgt atggcccgaa tgaatcaaag tcacatggaa
     3121 tatgtggtag atgtagatat caaaggtttc ttcgacgaag tcaatcatac taaactcatg
     3181 agacaaatct ggtctatggg tatacgagac aaacagcttc tcttaatcat taggaaaatg
     3241 ttaagagcac ccgtagtact tccaaatggg aagttgatat acccaacaaa aggaacacca
     3301 caaggcggaa tacttagccc attacttgcg aacatcaatc tcaacgaatt tgactggtgg
     3361 atagcaaatc agtgggagga aaaagatgcc aaagagctaa aacatcattt taggaaaaat
     3421 ggagcttggg ataaaggtaa tatctatcac aagctcagaa agagtacaac tctaaaagaa
     3481 atatacctag tgagatacgc agatgatttt aaaattttct gtagaaatta taatgaagcg
     3541 aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc gtctaaagtt accaatatct
     3601 gaagaaaagt caaaaatcac aaatctttca aaagaagaaa gtgaattttt aggctttacg
     3661 atgaaattag tgaccaaagg taacaaaaag gttgttaata cgcatatagg tacaaaagca
     3721 caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac gaattcaaaa aagtcctaat
     3781 agcaaagata cctttatcta tattggagtc tataacagta tggttattgg gattcataat
     3841 tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa aaattgcaag agaactcaga
     3901 ccaagttttg aaatacgact acgagaacaa ggaatctcaa aagaaggaca atacggaaag
     3961 tacgaaggat acaagcctta cctacggtca ggacaaatac gatatataag agggtttcca
     4021 atattaccag tagggtatat tcaacacaga catccgatgt gtaaaaaaag aaatattaat
     4081 aaatacaccg aagaaggtag ggaagccatt cataagaagc aacaaaccgt agaagaatgg
     4141 aagatatcgt ggttgtgtag caatccagtg cttagtaaga gagcaacgat tgaatttgcg
     4201 gacaatcgaa tttcaaaatt catcgctcaa aagggaagat gtgcagtaac aggtgaggag
     4261 cttatcttat ctgaaatgca ttgtcatcat attatccctt atcatgaaag caaaagtgat
     4321 agctatgaaa acctagtgat tgttactgag gaggttcatc gggtaataca cgctacacaa
     4381 agtgaaacga ttgaggaact tctaaaatat ctaaaattaa atccaaaaca aaaagaaaaa
     4441 ctcaatgagt tgagattaaa agttggtaac gaagaaatat cttaataaat atttgtagga
     4501 cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt atgcggtgaa agtcgcacgt
     4561 acggtgtgga ctgggggaaa agacggagat aatatcaaac tcttacctat cagtatcgat
     4621 taatccagat tttattgaaa cggtaaatta tgtcacaaac tttcaacgag aaatgcgtaa
     4681 agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat
     4741 gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt
     4801 tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagtgggta aatcaatgac
     4861 gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg
     4921 ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga
     4981 tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca agcagtatgt taagcgtcgt
     5041 gcaaagcggc gcttattttt gtggttgttt ggtacttctg caggtttgat tactattttg
     5101 atcactgtgt tcgtgaccct atttctaata cttgcggcgg gctcgattga taattcggat
     5161 tcagattcaa gcagtggtgg cgaagctttt acaggagaat attcggaagg cttaccgatt
     5221 tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata tgccgtaggt
     5281 gcagcagtga aatataaatt attaccaagt gtgatccttt cgcaatacgg ttacgaatca
     5341 gcgtttggta catcggcgtc cgcaagaaat gatctcaact attttgggat tacttggttt
     5401 gatggttgtc tgtttcctaa aggaactgca cgaggaattg gtggcattga aggtggctgg
     5461 tacatgaagt ttcccaactc aaaagctgca ttttcctatt acggctttat ggtagccaca
     5521 caatccaatt ttaatgcttg tgtaggaaat aaaagccctg gcgcaagctt attgatctta
     5581 ggacgaggcg gttatgccgc tgcaggaatt acggaagata gtccgtatta tacaggttgt
     5641 atgtcaatta tcacttctaa taaattaacc gaatatgatg aattcgccat caaacattgg
     5701 ggtgaaggtg gcaataacaa cggcacgatt actggtgaat ggacgaatcc attccctggc
     5761 agcagtttag ataaaagttc cttcagcggt ggtcaattat ttggaacgaa tccaggcggt
     5821 gaattccgtc cgaacggctt ccatgatgga ttagattttg gttcagttga tcatccagga
     5881 agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca
     5941 ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt gtatcaagaa
     6001 tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa
     6061 gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat ggattggcgt
     6121 caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt accatttttg
     6181 aatagcagta aaaaatagat caggagaaaa aatatggaaa ataaaacgtt aaaaatcgtc
     6241 ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg gcagctgaaa
     6301 gaggaacaag cagcaaatac ctataaaatt gaagaagtgg ctaaaaacgc gtctgagaag
     6361 ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca agttaaatca aaccatcaat
     6421 gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc aaaagaggaa
     6481 gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat tgataaatca
     6541 ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga agtgatcgac
     6601 aaattaagtc ctacagacga tgatcaccaa catgatgatt acggaagtta ctcctttaca
     6661 ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc
     6721 gtatttgttg attatacaat cggcaatccg caattcaaag atgtcaaacc acaaaaaata
     6781 aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt catagacttt
     6841 agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagaaa gacaacgttt
     6901 caaaaaacga aaaagttatc ttagatggct gggaatcttt atgattctcg gctttttgat
     6961 tcttgctagt ccgatttata cgaatcagaa ggtagatata ccagatgagg taagcacgaa
     7021 cgccttttta aacacgaaag agctgacaat ggtttcaaaa gaaaaaggag cgaaccagca
     7081 attagttttt gaatttgcga tcgatgatga agatcaaagc ttgttaaaag aactggctaa
     7141 tcttaattat cgcgtagaag taaagacagc taaaggggat tatcaagcaa tacgagccaa
     7201 agtgattaag gtgtctgatg attattttgc agtgaagcta acgcatgtac cagaagaata
     7261 tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg atatgcaaga
     7321 accacaagat ctgatttttt atattcacga agataaggtc aaagatcacg tgaaagacga
     7381 agattatgaa caacatgcga tcaattacaa agtgaaagga tacaagaaag aacaaaaagc
     7441 agctcaaaaa caaatggaat ctttccaagc aaccattgat ttgaatgaag aattagtgaa
     7501 gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata ctgaaaacaa
     7561 gattaatagc taccagcaag aaattgaaac atcgaaaacg caactgaaag atcaagaaga
     7621 gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga tttagaagga
     7681 caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt tcgctgagca
     7741 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcgagaagt
     7801 gaatttatct aacacaacaa tatctgagtt gaagaatggt cgaaagaatt tagatacctt
     7861 aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta cagctgataa
     7921 caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct tatgaatcag
     7981 ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac gattctaacg
     8041 caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga tttcaaccaa
     8101 acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt tttcatatga
     8161 caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta aatacacaaa
     8221 taaccttaat ttttaaagag cataaggaaa cgtgatatca ctttctcacg aaaggtgtta
     8281 ggtaggctgt gcctaccacg tttctaagaa agataaatga tatcaaaatt ttggatttgt
     8341 gtgtagattt gtgggtagga tgaaagcgaa tgtgggcagt aaaataagcg tttgtgtgta
     8401 gcaaaatcaa aaaatgtgta gggatatgtg tagctacaca tatccctaca cataataaat
     8461 ttggaggaaa atatggactt attaattaga aatattgaag aaaagtatat taataaaatt
     8521 gacaaacgtt gtgaagaact tacggtcaag actggcaaaa aatggagtcg aaatcaatac
     8581 ctaaaagttt tgatcgaaaa tgattttgat cacgctcttc tgaattataa aaaagaccaa
     8641 tttgatcggt tgttagaaaa atttgtggat attcaaactt ataatacgaa aactttagaa
     8701 gaatatattg caacaaataa tcaattaatt ggtttgttaa ttgaataata aaaatagtat
     8761 tgtgaattta taaaaggagg aaatcaattt tggcaaaagc aacaagaaaa catgtatatc
     8821 tttacgatat gcatatcgaa gtacttgatg aaataatttc tactcatgaa gatatatcta
     8881 attatagtga agcagtcaga catttgtgta tgatgtatga tccaaaaatc gaagaaaaaa
     8941 taaatccaat tcaaattaaa ttgaatgcaa tgggaaaaga aatttctatt ttaaccgaaa
     9001 tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt ggtacaaatt
     9061 caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc aatcaaacca
     9121 aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc agagtagatc
     9181 catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg atgaataaat
     9241 cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat
     9301 acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt acgcccgaag
     9361 aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag
     9421 aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat gaagctcaat
     9481 cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt accaaatatc
     9541 tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg caaagggaac
     9601 aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag ccacataaaa
     9661 aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg aaaggaagtg
     9721 atctgaagga gatcaaacaa atcttccaaa gaggacaaca aaaaggctca atcatttatc
     9781 aagatgttat ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac gatcccaaaa
     9841 cagatacctt agatgaagaa ggtttgaaag cagctggaag aaaaatgatg gagacattat
     9901 tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac cgtaacacta
     9961 agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccgtatactg
    10021 agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa gagaagacaa gcaacattag
    10081 acgcaatgaa aaccaattat gtacatgagt tagaacgttt ctcttatgaa aaggatcgaa
    10141 tgatcggtat ggagaaacga aatttattga cacgaaaaag tgatttgcgt aacaccttaa
    10201 caaaagtagt aaaagaacca gcacgttacg atcgacacgc catgaactta ttaaatgaag
    10261 tatataaaac cctacctgaa aaacgttcgg aatggaatta tggagatgaa aatcgaacca
    10321 agctttcacc atttacaagg ggaaaacttg atgagttaac caaatatgtt ttaacaaaag
    10381 aacctggata tcaagaatat atacagattt caaaagcatt gaaagaagaa gccaaaagcc
    10441 tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa aaacgccctc tttgatttaa
    10501 aaaagcgcac aggaaatgcg attttaacag aactgaagag acaggatagt caaataaaac
    10561 cattaataga aagtgtcaat cagatgcaat ctatttacaa tgaacgatcc tttcgaacac
    10621 tcaaacagtt gccaacaaat gaatacaagt atagttatca agaattcgta aagcgtagga
    10681 aacaattcca acaaagagtt gtcacgcagc gaagcttgcg tcaattaaat cgaacaatta
    10741 atgcacatga agaaaaatac cacgcaagta aagcatatga agagcaacag cgaagaatcc
    10801 aaatggaaca agaaagacaa taggaggaag agaaatgaaa caatatttag atcaatggaa
    10861 agtcatagaa ggatcactaa aagaagaacg aatcgaacaa cttcctgatt gtttagaaaa
    10921 agagcatttg tttcagataa gcgaaatgtt gagaaacgaa cagtttgatc caaatcaatt
    10981 tttagttgtt gagtacccag ctactggtgt gtattgttgt aatcatgtga agggtgaaaa
    11041 atattttatt attcaagaat acgaaggtaa gttagcgcct tattatacta cttgggaaat
    11101 aaatgaagag ggaatcaata atttcccatg tgaaagcatt gaagagtcta tttctctcac
    11161 ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg tgattgtcca ggactctttt
    11221 tcaatgaatt gttttccgcc aattcactga gtggtgcgat tcaattttat tatatcaaaa
    11281 gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga ctcagacaac gattggtgtt
    11341 gtggttgaaa aaagcaggga taatctcgtt tttgtgacag aaattcaaac aggtagagct
    11401 tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg gcaatatttt aacgttaaat
    11461 ctgactacga agatgttcgt tgatgcagta gaaaattatc catttgttta gacaaacgat
    11521 attttacaag gaggaaacgg acatggcaga agaaaaagaa ccactagata atagtcggtt
    11581 gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa ttaaataatc aatttcaaaa
    11641 gctatttgat caccaaaaat tgacaaatgg gcaaccaatg aatgacaaac gaaatggtcg
    11701 ttcatggttt aaccagcaag aaaaaataga aaataaaata caaagtttga gagaagagat
    11761 caagcaacaa gaaaaacagg tagaaaaact tgaaagacaa gaagagctaa aggaaatggg
    11821 ctataacaag tacggcggac tggatatgac gatcgataat attccaagaa tcaaagaaga
    11881 aatcgaacgc tttgaaaaag gtgaatcgac tttttcagcc gccacgatcc gaaaatatca
    11941 acggaagttg gaaacattag aacagctgaa agaaaaatca gaaaaaggaa aagaaaatat
    12001 cctccctgaa gtacaagcga tcattgacag cggacgagtg acgcaatgga aaaaaaatcc
    12061 aaccatttat tttttgaaag gctatcgaaa agttgcttta gaactgaatg aaaacggggg
    12121 atttgaggaa tcaaaaaaat accaagccac gtcggaagag gaaaaagcag ctgttcgtga
    12181 attacttgaa gaggctgcac caactgattc tctgaaggag aaagaaaaag atcatttcta
    12241 tcgagtagaa ttcaacgaaa atgatccaga aaaaggattg aaaagttatg gtggggagat
    12301 cgtaacatta gaattgatcg atcaacttaa aacgttggat aaccaatacg ctgcagaagc
    12361 aggttattat aaattctatt ttgaagaagt gaagaacgga gaagtagtag atcagatccg
    12421 aatggatcta ggggatggcg tcaacagtaa tcaccccatc tatttagagt tagcaaaatt
    12481 gtgcgaacaa gtacctgaga aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa
    12541 agaacaaatg cagtcgaaag aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc
    12601 agaagagatc aaacaagaag ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa
    12661 gcatacccat tcttctgaag acgtcaaaga acttttgaac tttatgtctc gttttccaga
    12721 acgatccttt agaaatcaat tattgattga ggaacaattt ccaggtgcaa cggcttgttt
    12781 aggtcgtgc
//
LOCUS       DAZKOM010000085        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000085 DAZKOM010000000
VERSION     DAZKOM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.085"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8NF_002176"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8NF_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0472642.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8NF_002177"
     CDS             complement(323..892)
                     /locus_tag="ACG8NF_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0472643.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8NF_002178"
     CDS             1078..1980
                     /locus_tag="ACG8NF_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0472644.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8NF_002179"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8NF_002179"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0472645.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8NF_002180"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8NF_002180"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0472646.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8NF_002181"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8NF_002181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0472647.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8NF_002182"
     CDS             8554..8739
                     /locus_tag="ACG8NF_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0472648.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8NF_002183"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8NF_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0472649.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8NF_002184"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8NF_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0472650.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8NF_002185"
     CDS             11896..12705
                     /locus_tag="ACG8NF_002185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0472651.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKOM010000086        12493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000086 DAZKOM010000000
VERSION     DAZKOM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.086"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8NF_002186"
     CDS             <1..375
                     /locus_tag="ACG8NF_002186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472652.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8NF_002187"
     CDS             complement(324..893)
                     /locus_tag="ACG8NF_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0472653.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2257)
                     /locus_tag="ACG8NF_002188"
                     /pseudo
     CDS             complement(967..2257)
                     /locus_tag="ACG8NF_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2515..3459
                     /locus_tag="ACG8NF_002189"
     CDS             2515..3459
                     /locus_tag="ACG8NF_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0472654.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3473..4546
                     /locus_tag="ACG8NF_002190"
     CDS             3473..4546
                     /locus_tag="ACG8NF_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0472655.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4536..6707
                     /locus_tag="ACG8NF_002191"
     CDS             4536..6707
                     /locus_tag="ACG8NF_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0472656.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6819..8990)
                     /gene="copB"
                     /locus_tag="ACG8NF_002192"
     CDS             complement(6819..8990)
                     /gene="copB"
                     /locus_tag="ACG8NF_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0472657.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9010..11196)
                     /locus_tag="ACG8NF_002193"
     CDS             complement(9010..11196)
                     /locus_tag="ACG8NF_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0472658.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11196..11405)
                     /gene="copZ"
                     /locus_tag="ACG8NF_002194"
     CDS             complement(11196..11405)
                     /gene="copZ"
                     /locus_tag="ACG8NF_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0472659.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11418..11858)
                     /locus_tag="ACG8NF_002195"
     CDS             complement(11418..11858)
                     /locus_tag="ACG8NF_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0472660.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11932..12430)
                     /locus_tag="ACG8NF_002196"
                     /pseudo
     CDS             complement(11932..12430)
                     /locus_tag="ACG8NF_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4126 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttt gatcttcgtt atggtctagt ggaaaaaaat
     1081 gatcggtgat cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag
     1141 agatccgttt tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt
     1201 ggtgttcata gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc
     1261 ctcgttcctg gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaaatg
     1321 cttgttggat cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta
     1381 atgtttcctg ataataacga acttcttcta tcagccggtc atggatacca attacgtaac
     1441 gaagccattc ttggtaaagt acttttgtca gctttgattt gttttcgctc tcaaattcgc
     1501 tattcagcca aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt
     1561 gtgtctcttc tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa
     1621 ttgctagata atttttttct tttattggca aaatctgctc ttctctaaat agcaattcag
     1681 gaaacaattc aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct
     1741 ttctttgctt caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa
     1801 aaaaatgcgg aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac
     1861 caaagatctg tgctttttta taagaaaaag aaaggtggta ctcgccaaac cactttctca
     1921 atcgctcttg cgtagaacga tcgtacttct gctcgtaagg ttcattcaat agcagatttt
     1981 tcagtaaaca aaacgggggt gattggatca gaaaaacaaa caagagataa ggttcgctat
     2041 atccttgatc ataaactaac tgaacggttt tcttatgaag aatcagctgg atgggacagt
     2101 cgctttccgc caaacgtttc agatgccgat ccactgtttt aacatcgatt ttcagttctt
     2161 cagctaaatc agcttttcgc cagatcaaat ctttcattag attgattagt gaaaataaat
     2221 tctgtgcatt ttttgataat agtttttgcc atctcatcga tcatttctca cctgatttct
     2281 aaacatattg tttgatcaaa atgggttcac tcctaaaaag ataaaaaact ccctttctat
     2341 ttatttaaat acgcaaattt tcctcattat ttttaatttt actcaattta atcgtattct
     2401 catttcaaaa cgaatgtact ttaaaaaaac ataataaaat cactttgctt gtaggatgta
     2461 ttcccttcca ttacaatgaa ataaatagca attttatgag aaggtgatgt gaaaatggaa
     2521 gaaaaaatcg aagaatccaa acaaaaaatc gatcagttga tacaagaatt agaaaaagcg
     2581 gtcatcggca aacgttcaac tatccaattg atgatcgtgg ccctattagc aggtggtcat
     2641 gtcttattcg aagatattcc cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa
     2701 gctgtacagg gaaccttcag cagagtccaa tgtactcctg atctgctgcc tagtgatatt
     2761 cttggttttt ccgtatacaa ttcacaatca aaagagtttg aatttcgtcc gggaccgatc
     2821 tttaccacga tcttgttggc agatgagatc aatcgcacga ctcctcgtac tcagtcagca
     2881 cttttagaag caatggccga gaatcatgca acgatcgaca acaatacata ccctctagac
     2941 aatcattttt ttgtcttagc tacgcaaaat ccaatcgagt atgaaggaac atatcctttg
     3001 ccggaagcac agctcgatcg ttttttattc cgactacaaa tcggttatcc ttcttttgat
     3061 gacgaacttt tgctactttt agataaattc gaaaaagtgt tggataactt aaatgaagta
     3121 ttgaacagtt atgaaataga agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc
     3181 gaagtggcat cttatgcctt gcagttagtg tcagcaacaa gaagccatgc tgccatccag
     3241 cttggcatca gtccacgagg aagcctttcc tttattcagg cagcaaaagc ttatgcttta
     3301 atccatggga gaaattacgt cacgcccaaa gatttgcagg atttagttcc ttacgtgttt
     3361 tcccatcgcc tgatttttta cgatcgttca ttgaaggaag acgaaaaaaa acaaatcatt
     3421 catactcttg ttgcacaaat tcctgttcct gtgaggtaga ttgaggtaga taatggaaaa
     3481 aatcaaagat tatttcatta attttgtgtt atttctggta tatttcatca ttgtgctttt
     3541 tgctcttgta ttcaacaatt ctttaggctg gttcttgttg gcctttttta ctctttatgt
     3601 atcatggagc ttgatttcat taagcagctt tctaaagaaa ctttcggtta cttgtacatt
     3661 gcagccactc gtttatcgca aaaaaagtat gcaggttatt ttttccatag caaaaaagag
     3721 aactttttct ttacctctcc ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa
     3781 aaaacaagag attttgattc ttacaaataa accgcaagaa gtccaattgc tttggcagcc
     3841 aaaagaaaga ggatgccatg aaacattaac tgtcattttt acagcctatg atagtctagg
     3901 acttttcaga aaaagtgcaa aaagagagat ctcttttcct gtgactgttc tgcctgcttt
     3961 ttgtaagatt caagcagaaa aattacagag agttctggaa aaaaaacagc agctgaacgc
     4021 gtaccaaaga ggtcttgatt ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa
     4081 ccgaatcgat tggaaattgt ctgctcatac tcaagagtgg ttataccgtg aatacgatta
     4141 tcaagaagaa gaatgttctc ctcttttttg cttttggggt tcgcacagtc caaaatttga
     4201 acctctgcta gacttgtatt tttctttatg gcatttgaga aaagaaaagc agccggaact
     4261 tctgatatta ggcaaccgcg cctttcatgg caaagacccc ggtcatacct attttgcttc
     4321 tttggaagct ggcgatgaga aagctttttt cgcacaccta aaaaaatatg caaaaaaaca
     4381 aatcgtcctc tttgttcctg agaattctcc tgaagtctca gaagctattg aaacattgtc
     4441 aaaagatcga acggtttatc ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa
     4501 agacaagata tgttcattgc caggaggtga atggattgac gattaaatgg gaaaaatggc
     4561 tgtgtggcgt ttactccttt gtgatactaa gtgtaggtgc tccgatattt gtagtagcta
     4621 atcaattgca aaacagctac cttcttatcc taactattgg tgtcctatgc cttattgtct
     4681 gctgtattcc taaaaaatgg gtacgtttgc tgctttcttt gccagtaatg ataggttgtt
     4741 tgtttttgta ttttccttcg aagacatttt ccttattatg gatcttacag tttttttcgt
     4801 cagtcacaga ggaattcttc catctatccc aaggtaaact ttattatttg ccagaaaaga
     4861 cagctttttt tctcataatg ttgctgatct atttatttat tacattgacc gtggattacg
     4921 actatttgta cgcacccttt ctttctttga tggcttactt catgatgctt accgtttttc
     4981 gggaaaaaga tttactgtac gaaatgattg cggtagtaac tgttttgttc gtgtatctgg
     5041 cagcaaggca atttttctcc atgcggttgt tgtcgggaaa cactcgattc cagtcattga
     5101 ctacagttct gcttatttgt ttcttaacta ttagcgctat cttgccttta tatcttccga
     5161 aagtacacca atctctcgag gaaacgagtg aaccgatccg agaatatctt aatgatgaag
     5221 ggctttatga tacactccat gagtatcaat taaccggaag tgcaagaaca ggcttcagtg
     5281 agaatgatga acagcttggt ggacctttat atgataatgg cgaagttgtt tttacagcga
     5341 accaaaaagg aaatggctat tggaagatcg aaaataaaaa tcattacacc ggttctggat
     5401 ggatagaaga tcaggacgca cccatccaac caatccaaga attccctgtt gacatcacga
     5461 gctaccaaac cgttatggaa gacaaagaaa acattacatt gaccctaaaa aaacaagctt
     5521 tcttacctta tccttatgga aatactacct tgactgattt ttcattagct agtggacaaa
     5581 cgctttacta tttgcctgaa agccagcgat tcattcctgt cccgaatgaa tcgacacgtt
     5641 ctgcaacttt acaaacagaa aggttagttt ttacgagaga agaattaaaa agcagtcagt
     5701 taaaagctga tgatttagaa aatcagcaga caaatcttca attgccaaaa agtttgccta
     5761 aacgtattga ggacttggcg gtcgaattga ccaaaaatga agcaacttta attcgtaaag
     5821 tcgaagctgt cgaacagtat ttaaaaacaa gcggaaattt ccgctattcc aaaacggata
     5881 ccggacatac tccgaaagaa cacgattatg tggattattt cctctttgat tcaaaagtcg
     5941 gctattgtga caacttttca acttctatgg tcgtccttct tcgaacactt ggtatcccag
     6001 ctcgctggac aaaaggtttt acaaatgggc tcagaacagg cacagataat caagggaatg
     6061 aggaatacag cattctcaat agtcatgctc attcttggcc agaggtatac tttcctggat
     6121 ttggctggct tccatttgaa ccaacccctt catttgccaa tccaatgggt tcacaaccag
     6181 ttgctgaaac aaccgaaacc gtccagcctg ctatcgaaca accaaatgaa agcgaaaata
     6241 tggcagaaac tatttccagt gagagatctg aggtcattcc atcagaagaa acaacactca
     6301 caccagctta taaaatcgtc ttattcagcc ttggggtgtg tttatacgtc ttgttgttct
     6361 ttctattgtt attcagaaaa tctctgcgct ggacaagtta tcttatctat cttcattttt
     6421 ttgcttctac taatacgatt gcatatcaaa aacttcttaa ccatttggaa acattggtac
     6481 cgcgcaaacc cgcagaatct ttgcaggaat atagcctgcg aatagaacaa gaattcaagc
     6541 tagatctgcg tttcattcga ttaactactc aatatgaagc agaaatatat ggacgacaag
     6601 cagatccggt aaatttcgat aaaaaaacct atttagaaat tacgcatata ataagaaaaa
     6661 cggtatacaa aaaagttctg acaaaaggga gccgcttcaa aaaataagat aaaatttctg
     6721 gagaatctac ttctggaatg ccgagaacca gctgcttgag tatcgattat tcgaaaaaaa
     6781 ggttgtgaca cgttttttgt cacaaccttt tttcattatt atttgatttt caatgtcaaa
     6841 gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac tgctggactt
     6901 aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag aatattgtaa
     6961 cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc tagatcaaga
     7021 aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga atcgattgcg
     7081 atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact tggcgcatca
     7141 ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt ctggacaaca
     7201 gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa ataatctgct
     7261 actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat ttttctttct
     7321 ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa agcaacgatt
     7381 gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc ttggtaaggt
     7441 ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc attaactatt
     7501 ttcacttgtt ggtttttgac tgttgcctct agcccaactc ctgaaagatt ttttagattt
     7561 tcggcttgat aaggcttgat ttcatgttct ttcaaataat tcataatacc aacagccaat
     7621 ggatgattgg cattcgcttc caatgctcct aaatattgaa ggatttcatt tttattgaac
     7681 gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag tgttcctgtt
     7741 ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg gtttttcaaa
     7801 agtaatccgt ttttagctgc tattgatgta gagcgagcaa caactaatgg gatagctaaa
     7861 cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa tgcattagga
     7921 agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa tgcgacataa
     7981 aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc tccttgtgct
     8041 tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc agttacttcg
     8101 atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt ggcttgtttt
     8161 ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc acctttcaag
     8221 attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc accttcgttt
     8281 acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt tactgattct
     8341 ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc attcatttca
     8401 atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa gaaatccatg
     8461 acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata aatatatgca
     8521 actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt caattccatc
     8581 ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc taagatcaat
     8641 acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc catcattggt
     8701 gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg tttgaaattt
     8761 cccatgtgca tggaatgatc catgccgcca tgatcgtgat ccatgtgcat cgaatggtcc
     8821 atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga atggtccatc
     8881 tggctatggt catgtttcat ctccatctgt ttttcatgag attctgtact ttctttgttt
     8941 actacttctt ttttatgttc atgttttttc tccatatcgt tcccattcat ttttactcca
     9001 tcccttctct tactttttca tgtgccgatt taagctcagg gagttcaaca atacactgac
     9061 ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta gtgcggcaaa
     9121 aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga tttttctcaa
     9181 tgtcacccta gacaactcga ttgtttgtgc aatggatgcc aaactgctac tcatcagcgt
     9241 cacatcggct gtttccatcg cgatatccgt tccacttccc attgccatac caacatcagc
     9301 taatgctaaa gccggtgcat cattgattcc atcgcctgcc ataccgactt ttttgccatc
     9361 tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag caaagatatg
     9421 ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt tgtctcctgt
     9481 cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt tggcttctag
     9541 tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag ctaaaaacat
     9601 gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat caaatgatag
     9661 gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt taatcgtacc
     9721 gctgattcca gcacctgggt gagctgtaaa atcaatcata tcgtatgttg ctgcttcttg
     9781 cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt gttctaaagt
     9841 ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg gctttccttc
     9901 tgtaatcgtc ccagttttat ccaaaacaat gctgtctaag tgagcagctg cttctaatgc
     9961 ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta ccataatagc
    10021 tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag agacactatg
    10081 gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg tgataaacgc
    10141 gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt tttggattgg
    10201 ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata ctgtctgctt
    10261 gccgacttgt gtgacttcca tgtgtagcag accattcgta ttgattgtcc cgccaaaaag
    10321 cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac tttcatctat
    10381 cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag gacggatgac
    10441 tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc cgtttcttat
    10501 cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac ctgttttcgt
    10561 ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca tactgctgct
    10621 ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa
    10681 ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg acttgattgc
    10741 atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag cagacagcac
    10801 taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat
    10861 agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa gatcgaatag
    10921 catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg cttcatcgta
    10981 caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag tggtcgtccc
    11041 ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga ccacaccagg
    11101 ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca tacctgtaat
    11161 gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc tttataaccc
    11221 aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc aaacttgacc
    11281 gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag tgcgcttact
    11341 gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga aaattgttgt
    11401 ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg gatacagtta
    11461 cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc attgatatct
    11521 tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat cctttttgca
    11581 cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc cgagacagct
    11641 ggatgataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt ttttaccaaa
    11701 cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc taagatctgc
    11761 gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat ctcccactct
    11821 gaatcactaa tctttactgg cgttttgact ttttccattt atttcacacc tttcgtttac
    11881 aaatgtaatc attaacttat gaatacagat tacacttgta aacaaaaact gtcaattatt
    11941 tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat
    12001 gatcagttta tttcttgcat tagctacttg gatcagctgc tgtatttttg gttcattgtt
    12061 tccttcaaaa tgccagataa aagataaaaa ttctaagtat ttcctgttta gtttctccga
    12121 aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta gagaacgcca
    12181 aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct tatctgcttc
    12241 tttaatcctt tcctctaacg tagaagtata atttccttca acgatccatg acggattaga
    12301 taccataaac gattgctgtt cttgcaaaaa ccactttttt gcctgaatgc ggaaagccgg
    12361 tgatttcttt gatcttcctt gcgagggacg attttcccga cccactttgt ccaatcacga
    12421 tgatcttcat tcttctccct cacttctctt tggaatttgg ttcgatcata ccacttataa
    12481 cgccgcaaaa aaa
//
LOCUS       DAZKOM010000087        12409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000087 DAZKOM010000000
VERSION     DAZKOM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.087"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8NF_002197"
     CDS             <1..154
                     /locus_tag="ACG8NF_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0472661.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8NF_002198"
     CDS             complement(208..486)
                     /locus_tag="ACG8NF_002198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472662.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8NF_002199"
     CDS             complement(601..1218)
                     /locus_tag="ACG8NF_002199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472663.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8NF_002200"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8NF_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0472664.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8NF_002201"
     CDS             2780..3196
                     /locus_tag="ACG8NF_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0472665.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8NF_002202"
     CDS             3193..3663
                     /locus_tag="ACG8NF_002202"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0472666.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8NF_002203"
     CDS             3677..4450
                     /locus_tag="ACG8NF_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0472667.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8NF_002204"
     CDS             4464..5276
                     /locus_tag="ACG8NF_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0472668.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8NF_002205"
     CDS             5330..6295
                     /locus_tag="ACG8NF_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0472669.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8NF_002206"
     CDS             6431..9154
                     /locus_tag="ACG8NF_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0472670.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8NF_002207"
     CDS             9167..9799
                     /locus_tag="ACG8NF_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0472671.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8NF_002208"
     CDS             10007..10231
                     /locus_tag="ACG8NF_002208"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0472672.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8NF_002209"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8NF_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0472673.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8NF_002210"
     CDS             10556..10825
                     /locus_tag="ACG8NF_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0472674.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG8NF_002211"
     CDS             complement(11004..12305)
                     /locus_tag="ACG8NF_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0472675.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4048 a         2010 c         2435 g         3916 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattca attctaacgg agcttctgta
    12361 tgttttgttt tttcatgctc aaaagggacc gatagaatga ttttctatc
//
LOCUS       DAZKOM010000088        12373 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000088 DAZKOM010000000
VERSION     DAZKOM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.088"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..439
                     /locus_tag="ACG8NF_002212"
     CDS             <1..439
                     /locus_tag="ACG8NF_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0472676.1"
                     /translation="LGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSVIYFALLL
                     GIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDD
                     APGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            448..675
                     /locus_tag="ACG8NF_002213"
     CDS             448..675
                     /locus_tag="ACG8NF_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472677.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1311..1682
                     /locus_tag="ACG8NF_002214"
     CDS             1311..1682
                     /locus_tag="ACG8NF_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472678.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1938..2180)
                     /locus_tag="ACG8NF_002215"
     CDS             complement(1938..2180)
                     /locus_tag="ACG8NF_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0472679.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2212..3114)
                     /locus_tag="ACG8NF_002216"
     CDS             complement(2212..3114)
                     /locus_tag="ACG8NF_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0472680.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3127..3315)
                     /locus_tag="ACG8NF_002217"
     CDS             complement(3127..3315)
                     /locus_tag="ACG8NF_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0472681.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3329..3892)
                     /gene="amaP"
                     /locus_tag="ACG8NF_002218"
     CDS             complement(3329..3892)
                     /gene="amaP"
                     /locus_tag="ACG8NF_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0472682.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3930..4823)
                     /locus_tag="ACG8NF_002219"
     CDS             complement(3930..4823)
                     /locus_tag="ACG8NF_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0472683.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4901..5824)
                     /locus_tag="ACG8NF_002220"
     CDS             complement(4901..5824)
                     /locus_tag="ACG8NF_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0472684.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5873..6223)
                     /locus_tag="ACG8NF_002221"
     CDS             complement(5873..6223)
                     /locus_tag="ACG8NF_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0472685.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6256..7158)
                     /locus_tag="ACG8NF_002222"
     CDS             complement(6256..7158)
                     /locus_tag="ACG8NF_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0472686.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7151..8008)
                     /locus_tag="ACG8NF_002223"
     CDS             complement(7151..8008)
                     /locus_tag="ACG8NF_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0472687.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8342..9151
                     /locus_tag="ACG8NF_002224"
     CDS             8342..9151
                     /locus_tag="ACG8NF_002224"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0472688.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9191..9688
                     /locus_tag="ACG8NF_002225"
     CDS             9191..9688
                     /locus_tag="ACG8NF_002225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0472689.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10073..10146)
                     /locus_tag="ACG8NF_002226"
     tRNA            complement(10073..10146)
                     /locus_tag="ACG8NF_002226"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10110..10112),aa:Ile,seq:cat)
     gene            10333..10656
                     /locus_tag="ACG8NF_002227"
     CDS             10333..10656
                     /locus_tag="ACG8NF_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472690.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10820..11074
                     /locus_tag="ACG8NF_002228"
     CDS             10820..11074
                     /locus_tag="ACG8NF_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472691.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11144..11389
                     /locus_tag="ACG8NF_002229"
     CDS             11144..11389
                     /locus_tag="ACG8NF_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0472692.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11465..11830
                     /locus_tag="ACG8NF_002230"
     CDS             11465..11830
                     /locus_tag="ACG8NF_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0472693.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11891..>12373)
                     /locus_tag="ACG8NF_002231"
     CDS             complement(11891..>12373)
                     /locus_tag="ACG8NF_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472694.1"
                     /translation="KKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELI
                     TKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIK
                     RSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA
                     "
BASE COUNT         3549 a         2319 c         2120 g         4385 t
ORIGIN      
        1 tttaggtata agtgccgtat ttattttgtt cggcgtactt ttttatactc agatcgctac
       61 aagcgaaaaa ggattcaatt ttgattggca gaccacatta tttgtttctg tcatttactt
      121 tgcccttctc ctagggatcg ttattgctta tttcttattg aaactattga aaaaaatcga
      181 agataaccaa gctttttcca ctcaaagctt aaacatacta aaaaaaatcg aacgaaccat
      241 tttgtttatt ggtttcgcct tttttggctt acttcctaaa tttatgaaag tagcggattt
      301 agacgatgct ccaggtttga tgttggtagg aatcgctgtc gtattcattc cttttgcagt
      361 atacacatta acaagcgtgc tggaaaaact gcttgaacat gcgattttac ttaagacaga
      421 tcatgatttg acagtgtgag gtgcgacatg gcaattatcg ttaatttaga tgtagtatta
      481 gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag tgggcattac gatggcaaac
      541 atctctattc taaaaaatgg aaaagcaaaa gccatccgtt tttccactct cgaaaaaatc
      601 tgccaagttt tggagtgtca accaggtgat ttgttgacct ataaaaatga ctcgaatact
      661 ataccagaag aataaaataa agtagaacaa acagtaaata aaaaataatc tgaatgatag
      721 atgtgtgaga cttttagaag ttatttacgt ctattattca gattttttat ttcgattgaa
      781 taccgtttta tctataaata agatatgtac tgactcatac aaagggggcc ttttcttctc
      841 catgtactat cacttttcct gatacaactg gtttcctgtt acgttgtagt tggatctgct
      901 tttagtataa tactttattc attccaccag actgatagca gtttgagcag tctaaataat
      961 catgtagtat tacaggccat tctgtttcat accttgaagt gcttaaaact tcagctaatg
     1021 gcccattgtg ttaactctta ctccattctt ttttagtaag ctcccatata tactgtaaag
     1081 ccttctatat attatgtcat gttgcttttt aacatttact atgtagtttt gataaaatga
     1141 tttgaataac agtgatggac aattcttttt tatttttaac attattttga taataatgat
     1201 tatttattta aaaatacatt agataacatt tgttacgaca cgaaaaagct tcacaaacaa
     1261 atatcgttat tttaaaatga gggcgaactc acacaattag gaggaatagt atgccattta
     1321 ttgtttactt ttttctttca ttactcgtga tctatattcc attaccaaca tttttcctgc
     1381 tcgtcaatcg tctagcagag caacatgata cgattgctgc tcttacgaaa ctttgtttac
     1441 ttctatttgt ttgtctagat tactttacga gctattattg gctaaaacag acaagagtaa
     1501 aaaagagata ttatttcttc cttgtctttt tcaatctctt tgagtttctg atccattgct
     1561 ttttacttca tcagtatcat acgatccatt tgtggatcat ctgggttttc aaagtggtcg
     1621 tattattttg tatgttcacc tttcctatct caagaaaatt tagggaggca ttgttttatt
     1681 aaagaagctt ctctgtatca ataaagacac atttaaggaa aaacttttaa aaatctaatt
     1741 gggaatttgc ccgcataaat aagaggctgt gacaaaagtc ttgtcacagc ctcttattcc
     1801 gtataaacag tgttcaaaag cttgcatctt cgataatatg cccaaccaaa tgaaaaatat
     1861 gagaagctat ttttgtttgg ctgttcttat tacttgatga agaacagctt tttcacgacc
     1921 tctttttaaa agtcttttta cagttttcga gcaaagaatg atacgattag tactaaaatg
     1981 atggcaccaa tcaatgatgg cacgattgcc attcctgcaa tacttggtcc ccaagaacct
     2041 aataatgctt gaccaagtgc tgatccaact aaacctgcta cgacatttgc aataatcccc
     2101 atcgaagaac ctttattcgt gattgctcct gcaattgctc cgataattcc tccgacgata
     2161 agtgaccaaa taaatcccat gtttcgttcc tcctaatgtt ttacttcctt attactgaac
     2221 ccgtggttct gtattttctt ttactttttg tgcgccgctt ccagctgctg attttacttt
     2281 atctgtttga ttcgaagcaa attcaccggt ttttttcgct gcgtcagtta ctttgtcttg
     2341 caccgtttct tgatcttttt caaattcttc ttttgttttg atgtccacta cgttagcgtt
     2401 gacttcgatc acttctaaat gtgtcatatt ttttacttct ttgccgatta cttcttgcat
     2461 ttcttcaaag attttttcga tatctttgcc atattccaca atgacatcca tatcaactgc
     2521 tacttgtttt ttaccgactt ctgtttcgat cccagccgtt acattatctg tattcacaag
     2581 ttttcctgcc acattagaga agaaaccacc gtttgcagat aataaaccgt ctacttgttc
     2641 aattgcgatt ccgatgattt tttgcacaac tttatcttca taattcagct caccgcgcat
     2701 tgaatcttcc tgttcagaat catgctttcc ttggttattt gtatgatcag aaccaagttt
     2761 tccttgtccg tttgtgtggt cagaactaag tttttcttgt gctttcgcat ggtcagaagg
     2821 atgtttttgc tcttccacag gtttagaagg atcgattgca tcatgttgtt ctggaacttt
     2881 agattctgga gttcctggat gatgaggtgc atgcttttct gcatcatgtt tcttctcttc
     2941 tactccttgt tgaacgttag ggactactgg atctggatgt gcagatactg gtgctgtttt
     3001 gttcttttca tcgttcacac catttgattg gtttggtgtc gttgggtctg gatgtgcaga
     3061 cactggtttt tggttgttca ttttattgtt attatttttg ttattgttat tcatggatgt
     3121 ttcctcctaa gatttaaaat agtgatctaa gattttcgtt tgtttcagat aaaaaccgat
     3181 agcgacgccg atcgctgcca tgatcaagat caataaggtc ttgaaaaagc ccacggtgat
     3241 ccataaaacg gctaatatca atccaagtac cccaaagatg atagggagct tgtatgtcga
     3301 aaatagttct ttcatttttg gtccccccct attctactcg tttgcggtca ttgcttcgtt
     3361 tagctggttc ttcaaagcct gtgtatttca ccgatacttt cactggctct tttgttccca
     3421 atatgtgttg tacttcattt tcgatttctt tcatcaatgt tcctgtttta ccaatgagtg
     3481 ctgaagttcg tttcaactcc ccttttacat tgacggcgat cttgtttttc gttgctttga
     3541 catttacttt tggtgaatgg atgaattcat cttcatgcaa gtgagaacga accattcctt
     3601 caattgcttt tttatccaac gtcaactccc catcaccatg tttcagtaga aagtttccga
     3661 ttgtttttgg ataaaacagg aaaacaagta acgtgataat tgtcaaaata gcaaatgcaa
     3721 cggcaagcca aaatacatat tgttgcacgt atgaattagt gatggaaaag aatcgaaact
     3781 tctctagctg aaaaggcaat ggataggttg cacttaatag tatcgcaaac aaaagcacag
     3841 atagcaaaag taatgttccg atcactccga tcgtttttac acctttattc attttcgcac
     3901 ctcctcatag gggtgaaacg atcaacttct tataagttga tcgcttctcc accagttact
     3961 ccgtaaattt gggctgtaac ataacttgct ttattcgatg ctaagaaaac ataaactggt
     4021 gctaattctc ctggctgacc tgcacgttct agtaaacttt gctgaccaaa ttcagggatc
     4081 gaatcagaag gttgtccttc gtctagttgt aatggtgtcc agattggacc aggtgctaca
     4141 ccatttacac ggattccttt aggtgcaagc tgttttgcta aaccgatcgt aaagttagcg
     4201 attgaagctt ttgttgctgc gtaatctaat aaatgttcgc ttggattgaa tgcttgaaca
     4261 gaagttgttg ttacgattgc tcctcctgct gggatatgcg gaacagcttc tttcactaaa
     4321 gcaaacatag cgatgatgtt tatcataaat gtgtctttga cttgctcaag tggtaactca
     4381 gcaatagact gacaagaaat ttgctgtcct gcgtttagta ccatcgtatc tagcccgcca
     4441 agttcactca cggctttatc tatgatttct ttaggtgctt gttcgtcacg aagatcaaaa
     4501 ggtagtaaga caatcttacg tcctgcatct ttcacgtatt ttgctacttc ctctgcatct
     4561 ttttcttctc ctgggaagaa ttgaagagcg atatctgccc cttctctagc aaatgcaatc
     4621 gctgctgcac gaccgatccc tgaatctcca ccagtgatta gtacacggcg gttttctagt
     4681 tgattattgc cttcatagga ttcttctcca caatcaggaa caggaatcat attctgctgt
     4741 aaagctggtg tatcctgatc ttgtttagga aatttctccg tatggtacaa gtttcgcggg
     4801 tcgattaatt ttggttctgt cattgctgta ttcctcctag tttttaaatt gtattgccaa
     4861 aacaaatttc tttgtttgat acctgtttta aatagattct ttaatttgta taatctttcc
     4921 ggtataaatg gtaacctaaa actactgctg ctatcaaaga gccaaagact acgagtataa
     4981 agcctagagc agaactttta cccggtaatc ctcctgtatt cattccccaa atccctgcga
     5041 acaacgccgg aacggaaatg attaatgcag ctgaatctag atatttcatg atatgattta
     5101 gattattgtc catcatcccg ttgaatagat caccaatact ctctaataaa tctcgataga
     5161 tctcgatcat tttttctgtt tgtcgctgtc tcaaatggac atcatagagt agctgttcat
     5221 cgtctaaacg tttgatgaaa gtttcagaat tccataattg atccattgtt tcccgttgat
     5281 ctcttaaggt atgatcgata tatacgatgt ctctttcaag atctgcctgt ttatacagtt
     5341 cttcattttc cgttgtttta cgagctgctt tatccaaagc gtcaattcgc tttttcatct
     5401 ctcttagttc tttcacgtaa tgggtgtaaa tatctaataa tgtatacgtg atgatctttt
     5461 caaacgagtt taaatgattt ccatgctttg tcagcagccg atccacaaat tcggtttttc
     5521 tatcgataca agtaatcaag agattatcgg aaagtacaaa agatattggc gttaattttt
     5581 tttcgatacg attatctgac tgatctagat taatcactac caaagaaaat ggatgctcta
     5641 gtttcgtctc ataaaggcgc tccatccgag aaacttcttc cggatattcg gttcctacga
     5701 aaatatcatc aggaagctcg aattcttcga ttgcttgctc aatctcttcg ggctttgcac
     5761 tatcaagtac gagccaatta tatttttttt catctgtttc tattacattc ttttcttcat
     5821 ccacaaagta gtatctcaat gtatacctcc tttttggact ttctcgaaac tattagctga
     5881 agaagcagtt ctttaatttg atattataat cgagtactgc gatttcattt tctaatgaaa
     5941 agctgtcaag ctgtttgttg aagtccatgt tcaatcgttt accgaaataa ttttgctcta
     6001 atttagatgt atagatgtcc aataatggat gctcgatttt agtcgcatat tctccaaatt
     6061 ccgaaacaaa aggaatgatt tgaaaaaagc tgccttttgc aaaagcacct ttgtccctta
     6121 gaaatggatc ggaatcattg ataagcgcga gtgcgatttt tgtattttcc ccaacctttt
     6181 caattaacgt ctctactgca tttttagaca ttgtaatttc cattcctact ccccctcttc
     6241 agttgttgtc ttctattatt taacaactac atctgttttt ggttgaactt ttgcatattt
     6301 tggatattta gggaatttag ctacacaacg gaaagccgtc atccctgcat atttttcgta
     6361 atatttcttc gttggccctt caaaatcctt gctccacggt ttcacatgtc ctgaaaaatg
     6421 gataatgtaa ggattgttgc gagtttcttc gtattcacgt tcacctgctg ctgttggatg
     6481 tttttttgct tttgccatga tatagccttg tgcattccaa cgcggatgaa gtggtaacca
     6541 acggtcatgt aagatagcat ttaatgcatc ttggtcatgg aatcgtaatt tttctggatt
     6601 atgttcgatg aaatctaata ctttttgcgt aatattttca tctaaccatt ttttcacatt
     6661 gatcagcatc aaacctgagt tgaaataacg cattgattta gctgaaattt tcattttttc
     6721 cagtcgttga tggaacccag cgtcttcaac agctgctacg attgaatcac cgaagtcaag
     6781 acgccataat ttagagatat cttgtaaagc gatcatgtcg caatccatat ataagatacg
     6841 ttcaacttct gtgccgcgga acaattccgg aatcgcaata cgataataag ctgtttctgg
     6901 aatacgatcg ctgattacca catttgtaaa gaattcttta ttgattttca agaattccac
     6961 atctgagttc attcgtgcgt ttttcactga gaagcgtaaa agctgtttac tttcaaaatc
     7021 gatatcatcg tcaatcacat agaaccgaac aaatttagac gggttgcaat tatctaatac
     7081 tgagacaaat aatgcagcta aatgtggtac gaattttgtg ttacaacttg cgactactgc
     7141 tatttctttt ttattcattg atctcgccta ctttcgttag agtactgtgt gctaattttt
     7201 ttaagtataa attggtatac ggatgatctt tcaacgtatt ccaaggttta tcatgtcctg
     7261 taaagtgaac gatcgaaggt ttttcatttc cttctttata ctgacgttca tatttttcat
     7321 ttggtgctgg atgacgttca aaaatcagcg aagtctgcat gttccatttt ggatgaagtt
     7381 gttcccaatc ttcataaagt acagcattca aagcatcttg gtcgtgatag atgattcgat
     7441 cgccattttc actgaggtaa tggatagttt tttctgtaat ttctttttta ttccattgat
     7501 caatgtcaat caccatcact cctgaattga agtacaagtc atcggaatcg attcctaatc
     7561 tttccaaagc tttcacttgg cctggatcga taatagcgcc gattgttttt ccatttaacg
     7621 gctcatcata tagttttacg atatcgtcta gtactaacac atctgaatct aagtaaagga
     7681 ctttcttata atcctcttta gctaataagt ttggtaaaga aattcttaag taagcggtag
     7741 ttgtgatatg gtcactgact aggaagtctt cgtagatatc tttttccact gtcaggaatt
     7801 ggatacttgc attacttgaa tatttattta ccgtttcttc caatccttct ttactatatt
     7861 ctgatagacc atcgtcaatc acataaaatt ttatgcgtct tgttttattg caattttcta
     7921 atgcagttgc gatcatcacg ctcaagtaag gagcataatt ttcgtctgaa gcagttacca
     7981 caggaactac gccgtatctc ttctccatca aaatcactcc tttttctaat ctttttctaa
     8041 cctgttctac atctagaata ttcctcattt ggttttaaag caaaatatat gctttgtttt
     8101 cattgaatat tttaaaattt ttttatcaca aaacgcatcg gtaagcgcta actattcgtt
     8161 tttcatggct ttttattcaa aggtattgac caaaaactac tatatatagc cacctttttc
     8221 ttaagaatgt tttttgaaaa aattcataat gacaaagcaa gttagtttca tttttttaaa
     8281 ttctcattta atcttaaggt aacaggaaaa tagatatgta aaaagaaagt aggaagataa
     8341 aatgaaacta gcagcgattg atttagacgg taccttgctt gatcgaaatg gaaagatacc
     8401 agaagaaaat atccaagcac ttcggaaatt tgatgaaaat ggcggaatcg taaccgtcgc
     8461 taccggaagg aacagcattt ctgctaaaga tattttttcc gaattaggaa tcggtggcta
     8521 tttgatttct tccaatggtt cactgattgc tgaaatgaag gatggaaaaa tggcgaaagt
     8581 tctcagacgt tcaaaaatag atagcgatac actgagaaaa gccttcttct atgctcaaga
     8641 agcgaaaata tccatcattg ctagccgaga aacccaagat gatcagctga cattcgatga
     8701 atcaaatcta gtcaaagatg acccttatta tgaacatttc aatttacaga accattcttt
     8761 tgatcaaatt ttaaatatgt tggaagatcc ttctcttagc tatcttaaat tagcattaac
     8821 agataagagt gaagaaaaac tacagaagct acaaagagat ctgcaagcag atggtatcca
     8881 atcagtattc tctgatccgc atttcttaga aatcacgcct acaggtatca ctaaagctca
     8941 ttcacttgtt tttctggcgg attaccttgg aatcgaatcg agtgacatca tggcttttgg
     9001 cgatcaagaa aacgacattg ctatgttgaa ttacagcggg ttgagtattg cgatggggaa
     9061 tgcacagcga caagtaaaag aaattgcaaa ccaagtaaca ctgacgaatg aagaagctgg
     9121 tgttgcttat attttaaaaa acttctatta aaaagaataa aatgacgata tgaatgtagg
     9181 aggaaagaaa atgttagtta ttattgatat gcaaaatcat attcttgatc caaacagcga
     9241 attttatatt ccagagtcag atcaattggc agaacgtatt gctcaaagat tagctaaagc
     9301 acgccaaaat aatgaatata ttctcttcac acgggatata ccagttgaac tgaaggacaa
     9361 agaagaagaa cgagaagatt tgcaattgat ccctcttctt tctccacaag aaaatgaacg
     9421 agtgattaaa aagtattact ttacgattcc acctgaaact ttagctgaaa tccaggaaac
     9481 tttatttgaa agcaaagaag aacaaaaaga gatcgaagta gtcgggattg aaacaaactt
     9541 gtgtgtactc tctaatctga taggcctgca gagtgctttt cctgaagcag acttttttgt
     9601 tgatccaatg ttagtctcca gtagaaaaca cggagaatcc gctttagagc tgttgaaaga
     9661 tttcaacgtc tctatcgtcg aatcataaaa atatctatct tcatttacga tatttaacac
     9721 acaataaaaa aagcacgcag aactgtttta gtcctacgtg ctttttctat caaagtagat
     9781 tggctttttt atgacacaga gtgaaacggc aggctcacga cctctttctt aaatggtgtt
     9841 caatcagctg accctcggta ttagcttaac aaacggcaaa atatgaggaa ctatttttcc
     9901 gtttgttaag ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggta
     9961 ttcaaaagct tgcatctgca gtaataagaa cagccaagta aaaacataag aagctgtttt
    10021 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tatgtacctt
    10081 gtaggactcg aacctacgac cggacggtta tgagccgtct gctctaacca actgagctaa
    10141 aggtacatca taaataaaat ttatttttgt tacttttcaa ctatagcacg aatgacaaac
    10201 tctcacaagt ttcaaaatat aagtaaaata atattgttat tcttatttct tttacttgtg
    10261 tcataactat tttgttactt taatttttat ttgcttattt tagaaaaaat gttattcttt
    10321 tatttattct caatgttagg agtgattatt ttgccattta ttgcgatttt attcgattta
    10381 gttgcggcag cagcttattt tctccaatta aattatcaaa cctcaggaat tctgtttatt
    10441 ggaatgattt ttcaaggtgt gattaccctt ttgcttcttg tcatgatgct cagctataaa
    10501 ggaaaaagat atgcccgtat tcaaacaaaa gtatttgtta aatatgtttc cattcgctat
    10561 gggatcatca ttctttcctt tttagtgaat gcgatcgtcc tctttttata tgtattgaat
    10621 tacttagata tcaatccatt gattttttct cgatagataa gacacattca gcggaacagt
    10681 tcctgcttat cagcagaaaa ctgttctgct ttttcatttt tcattgatct attcttcttt
    10741 tattttccta gcatttcatt tgtgatgtat gcaataacat gttatctttt attatatgaa
    10801 tcgttgagga gtccgttaca tgattacgtt ggtgagagtt ttgttttggg tgcctgcggt
    10861 cgcactggtc gcaagtattg tttatcttat gaattggaac aaagaacggt tttatttagc
    10921 tattttgact cttccggcta tctactttat gtggaaagtt tttaattaca actactttga
    10981 acccgatagt gtttttatag aagaattatc aggactggtt ttgtctttat tgatcgtcat
    11041 tctttatttg atacgtttaa ataaaaaaca ttgagtacta ttgattgcat gtgtagcttt
    11101 ctatagtcat tataactatt ctactataat gaaaggtgat taaatgagta tcgaagaagc
    11161 tattttactg gatactttac gttgtcgtct gaatttctta agaactggcc gatctttatc
    11221 tcaagtagct gtttctagcg cgttaagtat tcctttattc aaatatcgta agtacgaaga
    11281 aggagaagaa attccaagca tcattgaatt aatctatatc gctgactttt acgatatttc
    11341 cgttgattac ctaattggtc gctcagaaac agcaagcaaa ctgaagtgat tttttccagc
    11401 cttggtgctg gttttttcat ttaacgtcta aggttaacaa tctatatata aaggagacat
    11461 ttctatgcga ttatggcatg aaaaattgat ttcaaagctt cctcgtcaac agcttttagg
    11521 acaacatcgg gaatgctgtg cattaagagg aaatggctgg ggaaagaaac atgctactgt
    11581 ggattatgtt tttcactatt ctccttatag attattccaa tatcatcagc ttgtcatgga
    11641 tgaaatggaa aaacgtggtt acagcccagc accagagtgg aaaaatccta tgtatcgtgg
    11701 caaatcatgc gaagcatttt ttgatttacc tactgtagag cgcaacgtgc cgatttatcc
    11761 ggaacacgat gaagtttatc tagcagaatg tttagcaaat ttggagcaaa aaggaattta
    11821 tcttacctaa tataactaga aaagccttct atctcctctt acttcattaa aaagctttaa
    11881 ccatcttaga tcaagctaaa atgtcgtaag ttttagggtt atcttcatcc tcacgtgcaa
    11941 aactaaaagc gtactctagt tttggttcat cgccaacttc cctagacata aatgaacatc
    12001 tcctgacgtg gtttaaaagt accgcatctt cgacgtctgt atacgatgca cagcacagat
    12061 tgcttctttt gatctttctt ttgatttttc tgttgcttct tctacacctt cttcgattac
    12121 tacatcgatg cttacaattg cttctgccta atattcccat gcatcgtcga tttaagcctc
    12181 tgtagtcact gtgatcgttt ccacatttta actcctctgt cgtggcttca gttgattttg
    12241 tgatcagttc agttgctaag ccagagtcag aacaacctgc cattgctagc gttaatattg
    12301 atagtaaaaa tagttttttt atcataaggt tccccccctg aaaatagact aaaataataa
    12361 cgctttcttt ttt
//
LOCUS       DAZKOM010000089        12209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000089 DAZKOM010000000
VERSION     DAZKOM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.089"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..649)
                     /gene="frr"
                     /locus_tag="ACG8NF_002232"
     CDS             complement(92..649)
                     /gene="frr"
                     /locus_tag="ACG8NF_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0472695.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACG8NF_002233"
     CDS             complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACG8NF_002233"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0472696.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACG8NF_002234"
     CDS             complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACG8NF_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0472697.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACG8NF_002235"
     CDS             complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACG8NF_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0472698.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3631..4110
                     /locus_tag="ACG8NF_002236"
     CDS             3631..4110
                     /locus_tag="ACG8NF_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0472699.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4327..5418
                     /gene="phnW"
                     /locus_tag="ACG8NF_002237"
     CDS             4327..5418
                     /gene="phnW"
                     /locus_tag="ACG8NF_002237"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0472700.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5411..5539
                     /locus_tag="ACG8NF_002238"
     CDS             5411..5539
                     /locus_tag="ACG8NF_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0472701.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5543..6088
                     /locus_tag="ACG8NF_002239"
     CDS             5543..6088
                     /locus_tag="ACG8NF_002239"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0472702.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACG8NF_002240"
     CDS             complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACG8NF_002240"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0472703.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACG8NF_002241"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACG8NF_002241"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0472704.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACG8NF_002242"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACG8NF_002242"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0472705.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACG8NF_002243"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACG8NF_002243"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0472706.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3344 a         2529 c         2203 g         4133 t
ORIGIN      
        1 taattttttc tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg
       61 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
      121 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      181 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      241 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      301 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      361 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      421 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      481 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      541 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      601 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      661 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      721 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      781 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      841 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      901 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      961 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
     1021 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1081 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1141 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1201 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1261 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1321 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1381 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1441 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1501 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1561 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1621 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1681 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1741 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1801 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1861 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1921 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1981 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     2041 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2101 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2161 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2221 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2281 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2341 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2401 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2461 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2521 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2581 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2641 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2701 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2761 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2821 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2881 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2941 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     3001 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3061 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3121 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3181 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3241 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3301 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3361 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3421 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3481 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3541 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3601 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3661 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3721 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3781 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3841 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3901 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3961 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     4021 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4081 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4141 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4201 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4261 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4321 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4381 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4441 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4501 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4561 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4621 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4681 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4741 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4801 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4861 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4921 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4981 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     5041 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5101 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5161 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5221 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5281 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5341 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5401 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5461 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac
     5521 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5581 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5641 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5701 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5761 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5821 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5881 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5941 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     6001 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6061 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6241 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6361 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6421 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6481 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6541 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6601 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6661 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6721 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6781 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6841 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6901 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6961 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     7021 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7081 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7141 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7201 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7261 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7321 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7381 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7441 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7501 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7561 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7621 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7681 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7741 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7801 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7861 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7921 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7981 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     8041 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8101 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8161 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8221 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8281 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8341 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8401 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8461 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8521 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg taatttatt
//
LOCUS       DAZKOM010000090        12130 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000090 DAZKOM010000000
VERSION     DAZKOM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.090"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1015)
                     /locus_tag="ACG8NF_002244"
     CDS             complement(233..1015)
                     /locus_tag="ACG8NF_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472707.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1080..1916)
                     /locus_tag="ACG8NF_002245"
     CDS             complement(1080..1916)
                     /locus_tag="ACG8NF_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0472708.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="ACG8NF_002246"
     CDS             complement(1934..3343)
                     /locus_tag="ACG8NF_002246"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0472709.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="ACG8NF_002247"
     CDS             complement(3357..5180)
                     /locus_tag="ACG8NF_002247"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0472710.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6023..6349)
                     /locus_tag="ACG8NF_002248"
     CDS             complement(6023..6349)
                     /locus_tag="ACG8NF_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472711.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6395..6757)
                     /locus_tag="ACG8NF_002249"
     CDS             complement(6395..6757)
                     /locus_tag="ACG8NF_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0472712.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6794..7008)
                     /locus_tag="ACG8NF_002250"
                     /pseudo
     CDS             complement(6794..7008)
                     /locus_tag="ACG8NF_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7136..7471
                     /locus_tag="ACG8NF_002251"
     CDS             7136..7471
                     /locus_tag="ACG8NF_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0472713.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7547..9544)
                     /locus_tag="ACG8NF_002252"
     CDS             complement(7547..9544)
                     /locus_tag="ACG8NF_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472714.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9642..9917)
                     /locus_tag="ACG8NF_002253"
     CDS             complement(9642..9917)
                     /locus_tag="ACG8NF_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472715.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10149..10883
                     /locus_tag="ACG8NF_002254"
     CDS             10149..10883
                     /locus_tag="ACG8NF_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0472716.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10922..12064
                     /locus_tag="ACG8NF_002255"
     CDS             10922..12064
                     /locus_tag="ACG8NF_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0472717.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3935 a         2418 c         1848 g         3929 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
     5761 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata
     5821 ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa
     5881 taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa
     5941 tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga
     6001 cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca
     6061 catatatacg gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt
     6121 ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat
     6181 cacatgatcg atttaagttg tactgttctt gtaaaatctg tgttaccaat ggtttcgatg
     6241 acttctctaa tccagatgta cctgtcattt ttgaaaaaat gatacctata atagccaaga
     6301 caacgataac tataatgata aacttcttgt tcatatttac tttattcaac tttttatctc
     6361 ctatctccaa aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta
     6421 caccaaagat tgctccagct acccctgcta tcatcaaaat cgtacccatt tgttcatctt
     6481 tatctcgata taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg
     6541 ctaacgctac taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc
     6601 catgtacgat ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgcgt
     6661 ctgctccagt tgtctcatct gtttcttgta gtgctccgca aaactggcaa tacttacttt
     6721 catctgttat ttctttgcca cattttctac aatacatgtc ttctcctcct aaaaacattc
     6781 aattgtttat tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg
     6841 gtacacttaa tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg
     6901 tttgccacga acttacacat tgctgtgtaa cattcatttt tttgctaact ccgtttgaga
     6961 aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc
     7021 ctgttttatt atatacaaca atatgatgta tttcaactca taaaatataa aaaaacttca
     7081 aataccatta caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa
     7141 acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa
     7201 agaattagct gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt
     7261 aacagagcct aacctcgata gcttaaccgc tctttctaga gccctaggaa cccctgtaag
     7321 ccattttcta gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc
     7381 tcttagcact gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga
     7441 aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg
     7501 tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa
     7561 ttgggtcttt tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat
     7621 aaagaggaac taattcaact cctgataata tcaattcttt tattacttca tctatccctg
     7681 ctccttttgc taggtagact aaaaaactac tacttgcttt cacattgtat agtctcttaa
     7741 tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgttttctt
     7801 ttaaagcatt ttccacgctg ttattcccaa cattacgtag acgttcacaa acagtgttag
     7861 taagttcttt ctctgtcatt agatatgttc cttgccacga taagccctgt aaaacttcat
     7921 gtgcttcatc taaaatcaat tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa
     7981 atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt
     8041 gatatgtaaa atgaaatttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca
     8101 cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc
     8161 ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt
     8221 catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt
     8281 tatctatgtc ataagtttta ccatagtaaa aactcatttt tttagctaat tgttcccccg
     8341 acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga
     8401 catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt
     8461 tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg
     8521 taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct
     8581 ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac
     8641 aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt
     8701 tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta
     8761 acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa
     8821 accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat
     8881 tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa
     8941 aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat
     9001 ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg
     9061 ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt
     9121 cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt
     9181 gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct atgcttaagt
     9241 atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat
     9301 cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg cctttattct
     9361 ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg
     9421 taacttctct atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa
     9481 ctggcttctc taataatttt tctttatcta cataaattgg aatattaatc attgatgttt
     9541 ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa
     9601 caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc
     9661 atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt
     9721 aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag atattcactt
     9781 gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc
     9841 tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca
     9901 atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga
     9961 attatcttcg tattttttgc tcaaaataaa agtattttgt tattaaccga ttaatcaaac
    10021 aaaaaatgat taccagttca tcgacataca cataatacct cgataaaaaa gaggtgtcaa
    10081 tatctttgta aaatttattt acaacacccc tttttcgagg tatactaatt ttaggaggtg
    10141 tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa
    10201 caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga
    10261 ttaacaacgg tcaattaatt gacaaaaaaa ctatctacga atgggagaag ggaacctatt
    10321 taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt
    10381 tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg
    10441 tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc
    10501 caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga
    10561 tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt
    10621 tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa tttgacaata
    10681 acattctttt cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc
    10741 tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata
    10801 atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa
    10861 tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt
    10921 catggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg
    10981 acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag
    11041 tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt
    11101 taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca
    11161 gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat
    11221 acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt
    11281 ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca
    11341 aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact
    11401 tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa
    11461 taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt
    11521 attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga
    11581 cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt
    11641 tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac
    11701 tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata
    11761 caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc
    11821 aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac
    11881 acctcatggg tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa
    11941 ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca
    12001 tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca
    12061 gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga
    12121 acccttatat
//
LOCUS       DAZKOM010000091        12054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000091 DAZKOM010000000
VERSION     DAZKOM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.091"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..2251)
                     /gene="glyS"
                     /locus_tag="ACG8NF_002256"
     CDS             complement(176..2251)
                     /gene="glyS"
                     /locus_tag="ACG8NF_002256"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0472718.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2253..3170)
                     /gene="glyQ"
                     /locus_tag="ACG8NF_002257"
     CDS             complement(2253..3170)
                     /gene="glyQ"
                     /locus_tag="ACG8NF_002257"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0472719.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3556..4374)
                     /gene="recO"
                     /locus_tag="ACG8NF_002258"
     CDS             complement(3556..4374)
                     /gene="recO"
                     /locus_tag="ACG8NF_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0472720.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4517..5416)
                     /gene="era"
                     /locus_tag="ACG8NF_002259"
     CDS             complement(4517..5416)
                     /gene="era"
                     /locus_tag="ACG8NF_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0472721.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5431..5826)
                     /locus_tag="ACG8NF_002260"
     CDS             complement(5431..5826)
                     /locus_tag="ACG8NF_002260"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0472722.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5810..6286)
                     /gene="ybeY"
                     /locus_tag="ACG8NF_002261"
     CDS             complement(5810..6286)
                     /gene="ybeY"
                     /locus_tag="ACG8NF_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0472723.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6315..8507)
                     /locus_tag="ACG8NF_002262"
     CDS             complement(6315..8507)
                     /locus_tag="ACG8NF_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0472724.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8531..9502)
                     /locus_tag="ACG8NF_002263"
     CDS             complement(8531..9502)
                     /locus_tag="ACG8NF_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0472725.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10207..11586
                     /gene="brnQ"
                     /locus_tag="ACG8NF_002264"
     CDS             10207..11586
                     /gene="brnQ"
                     /locus_tag="ACG8NF_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0472726.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11868..>12054
                     /locus_tag="ACG8NF_002265"
     CDS             11868..>12054
                     /locus_tag="ACG8NF_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0472727.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3294 a         2563 c         2197 g         4000 t
ORIGIN      
        1 attataatta atcgttttca tttttatttc ctcacttttc atgagcgttt tgaaaattcg
       61 aacaaagaat aaaataacaa aaattattaa aatagaatta ataacagcta tttataacaa
      121 aaaaaacaga gacacagcac gtttcgtgca tgctctctgt ttttcaaaca aaagactatt
      181 ttgtgatgat ttgatccaag cttgctaatg acaaagccat ggcagctatt tgacttagtt
      241 gtgttagtcg gttcttacgt actgctggtt catcagccat gaccatcgtt tgatcaaaat
      301 aattttcgat cagtggacga agagcgacaa gctggtcgta gttttcgtgg atcgtttggt
      361 cagcaaaact tgctttcatt gcttctactg ctttgtgtaa ttctttctct gcgtcgtttt
      421 cgaacagctc agggtctact tctgattgtt tttcaagctg cgcttttttc gctaagttca
      481 cgacacgtgt caaagcttcc attgaaggtt tgaagtcttc atctgcaaga tgttgtttga
      541 acaattgtgc tgctgcaaat acttttgtca gatctttttg ttccgcattc aaaacagcat
      601 cgatgacatc atgacgaata ttcttcgttg tcaataattg gcgcagacgg cctttgataa
      661 agtcgatcac ttgttgttgc ccttctgata aaccaatgcc gaaacgatcc gtatctttac
      721 tgataacatc gctaatcgtt tcttgcagaa cagacaaagg aaaggcccat tttttcgatt
      781 cgatgatccg aacgacaccg tatgcttggc gacgtaaggc ataaggatcg tttgacccgc
      841 ttggaatcat cccaactgca aagaaagaaa agatgctatc taatttatct gcaatcgcta
      901 aaacagaacc tactgtagac tgaggtagct ctccttcact ggatataggc atgtaatgct
      961 cacgaatcgc tgtcgctaca gcaggtttct cgcctttcag aagagcatat ttttcgccca
     1021 tgatcccttg tagttcagga aattccccta ccatgttggt tactagatca aatttataga
     1081 tttcagcagc tcgttgcaaa tccatcaatt cttcctcaga tagtcctact tgttcgccaa
     1141 tgatacgggc aatgacttgt acccgttgca ttttctcata gatactaccg attttttcat
     1201 ggaaagtaac agtttttagt ttttcaacgt attggtcgat tgaagctttc ttgtcttcat
     1261 tataaaagaa ttccgcatct tctaaacgag ccgtcaatac tttttgattg cctcgaacca
     1321 catcttccaa ttttacccga tcaccattac gagcagaaat aaaatgagga agcagcatac
     1381 cttgatcatt ccgtacctcg aaataacgtt gatgttcttt catagaagtg actagtactt
     1441 cttcaggaac agaaagatat ttttcttcga agtcgccaga aaagacagtt ggatattcga
     1501 ctaaattgtt gacttcttct agtaaatctt cgtctagatc aataacccag tgattttttt
     1561 ctgctaattc gtttgcttgt gtaacgatca tttcttgtct tttttgcgga gacgcaatga
     1621 cgaattgttc attcaatttt tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc
     1681 ctaagaaacg atgacctcga gaggctctgc ctgttttgac atccaagatt tcgaatggga
     1741 ttacttcgtc atctaacaat gccacgatcc agtgaatagg gcggatatat tcaaaatcgt
     1801 attctgccca atgcatcgtt acagggaaag tcaaactttt gatcacccct gctaagcctg
     1861 tcaatacttc ttttgcttcc ttaccatgag tgtatttcgt tacatagaca tattccacac
     1921 cttttaattc tctaaaagtg atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt
     1981 ccgctgcttt cgtccagttt ccatctgcgt ccaaagcaat ttttttggct ggtcctttga
     2041 cttcttcttc actgtccgct tgtcgttctg gaatatttgt tactttgatt gctaaacggc
     2101 gcggtgtgga aaatgtttcg atcgtatcat aagtcaattg gttttcatct aaaaattttg
     2161 ctgttttttc ttctaattgt ttcatgcttg gtgtgacaac atgggcaggc atttcttcca
     2221 gcccgatttc aagtaataag tcttttgcca tcttattcag cctccttcac ggtttctgga
     2281 tttgctgtgt ttttattcaa caaagggaat cctaattttt cgcgttctgc cacaaagatt
     2341 ttggctacag cacgagccat gttgcggatg cgagaaagat aaccggcacg ctctgttacg
     2401 gatactgcac cgcgggcgtc taataagttg aatgtatggc tacatttcaa gatataatca
     2461 tacgccggat gaacaagatt ttcatcgata cagcgtttag cttctttttc aaaacgatca
     2521 aagttgctta gtagcaagtc ttggtcgctt acttcaaaag aatatttgga atgttcatat
     2581 tctggttgtt tgaagatctc gccatatttc acgccatcag cccattctaa atcatagaca
     2641 ctttcaactt cttggatata agaagcaaga cgttctaatc cataagtgat ctcagaagtt
     2701 actggtttac aaggcagtcc accaacttgc tggaagtacg tgaattgtgt gatttccatt
     2761 ccgtcaagcc atacttccca acccacaccg gcacagccca tggatgggtt ctcccagttg
     2821 tcttcaacaa aacggatgtc gtgttctaat ggatcgattc ctaaaaggcg caagctttct
     2881 aaataaagct cttgaatatt ctcaggtgaa ggtttcatca ccacttggaa ttgatgatgc
     2941 tgatacaaac ggtttgggtt ttcaccataa cgtccatcag ctggtcggcg agatggctct
     3001 acgtaagcag cattccatgg ttctggtccg attgcccgta aaaaggtata tgggctcatc
     3061 gtacctgctc ctttttctgt gtcataagct tgcatcagca tgcagccgtt tgatgaccaa
     3121 aatttttgta atgttaaaat catatcttgg acggtcaatt ttttactcat ctgttttgcc
     3181 cctttcaatt ttaaagcgtt gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat
     3241 ccctatgtct gattacagac atagggacga ataattcgcg gttccaccct acttccgaat
     3301 aaagattcgg cagcttcatt tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc
     3361 cggctcccac tctcccgaac tcgctaaacc acgtgttttt tactgatctt ggtcaatgta
     3421 cgtattcatt ttgcagtttt cttgtttcta taatagatcc tatcactgaa cgatctattt
     3481 aatctatggt taataataga cggaactccc tgtgtttgtc aaatggtttt gattttttgt
     3541 tgtatgtttt atttactatt cttctttttc aggtgtctct ttttcttttt cagacatgtc
     3601 tttctctgac gtctttaata cgtgtgccca agacttcatt tgattgatga actttttgct
     3661 tttcaaatgg ataccgacat attcatcata cagctggtcg atgagttcac ggattgcttg
     3721 tttggtttct tctttcagat ggattccact aattttatca taagaaatag ctgaaaacat
     3781 ccgaacgaaa taaacagctc gaggatctgc atgataacgg tgatcgtcca tcgaccaatg
     3841 tctttcgcat atcacaccac catatttgga agaatagtca aattttcctt ttgtttcccc
     3901 gcatacgacg cagtgtgtcc agattggtgc aattccgaag cgttgcagta tttggacttc
     3961 aaaaatattc gtgatgattt ctgcatcgtc tccttggtcc atccgttgca gtgcttgttg
     4021 cgtaaactgg aacaaatgcg ggtcgtactg atgatcttcg attgccgcat ctactagatt
     4081 caataaatat gtgccatagc cgctcaaaaa gatgtcttct tggatgtgtc gaaacgcatg
     4141 gacatctttg acactgctta aaaatgacaa accttcactt cgaaaattcc cgatataaac
     4201 tgcttcagta aaagggagca aagccggagc taagggctga ttttgccgat ggatctcttt
     4261 gatataaaac atcattttgc cagaagattc tgtaaagatt ttgactaatt tatctttttc
     4321 tttgtaatta cggctagata aaatcaagcc ttttgattct gcaagctggc tcatattctc
     4381 cctcctaaaa aataaatctg gaaaagcaga ttctgccgat ccagatcatt tatgttgcga
     4441 tcttccgcag cttttctcaa cacgcggatt tttattcagt ttatttctat acctaattta
     4501 caagaagctc agaatatcag tattcgtctt ttcggtagcc gaagtcttgc agatagactt
     4561 ttttgtctcg ccagtctttt tgaactttga cccaaagttc tagaaagact ttgtctccta
     4621 aaagattttc gatatctttt cttgctttcg ttcctacttg cttcagcatt tttccgcctt
     4681 tgccgataat gatccctttt tggctatctc tttcgacaat aatcgtagcc tggatatgga
     4741 ttttatcatt ctcgtttcgc ttcatcgaat caacaacgac tgcgacagaa tgaggcactt
     4801 cgtctcgtgt taaaaacaag actttctcac ggatcaattc agaaacgatg aagtattcag
     4861 gatgatcagt cacttgatca tctggaaaat attgtgggcc ctctggcatt tcactgacta
     4921 atacatccat caaacgttcg acgttgtttc cttctgtagc agagattgga atcacttgtg
     4981 caaaaggcat ctgcgttgaa taatcttcga tgattcccaa caactcgtct ggatggattt
     5041 tgtcgatttt attgataatc agataaacag gagaatgcac gttttttaga cgttcaataa
     5101 taaaatcgtc tccccgaccg cgtttttgat cagcactaat cataaataag accgcgtcca
     5161 cttctttcaa cgcactgtaa gctgcttcta ccataaaatc tcctaaacga tgctttggtt
     5221 tatggattcc tggtgtatca ataaaaatca actgagcttc tggtgtagtg tatactcctt
     5281 ggattttatt tctagttgtc tgtgccttgt cactcatgat ggcaattttt tgacccacga
     5341 ttcgattcaa taatgtggat ttccctacat ttgggcggcc gacgattgcg acaaaccctg
     5401 atttatgttc tggcatatat gttctccttt ttaattaaac aacaaatgat atattttagg
     5461 gacaaataaa atcaacccaa cgatgacggt aaaacaagcg gtcactaaaa ctgctccagc
     5521 tgccatatct ttgatttttt tcccaatcgg atgaaaatga aaatccgtaa acatgtccac
     5581 gacattttca aaaacagtat tgatgatctc tactatccat acgagaaaaa cagctaaaag
     5641 cagccataac cattctatcc gatcaagttg gaaaatcacg cccataaaca aagcgaggat
     5701 tccgaacgca acatgtttcc tcatgttgcg ttcttcctca aaaacagttt tgactccttg
     5761 gacggcaaat tccacggaca tcaggaaatg tttgtttttc tctacgtttt tatcttttaa
     5821 gtccataggc atctaagatc tctttctgta aaccaaacat ttctttctca tcctctggtg
     5881 tcatatggtc atacccgttg atatgcaaga atccatgaac cgctaaaaag ccaagttccc
     5941 gatcatacga atggccgtaa gcttctgctt gctctgcagc acgttcagta gagatcatga
     6001 tgtctcccag tacacgaggc aatggattct ctccatcttc aaaaatcact gggatttcgt
     6061 cttcgccctc ttcttccaag gcaaaactga tgacgtctgt cggtgcatct tttccacggt
     6121 aatcacgatt gattacttgg attgctgcat tgtccatgaa agtaacagac atttctgttt
     6181 cttctggcaa ctctaaatat gatgctgcaa attgcagcaa gtcgtcgatt tctttcattt
     6241 tctcttctga gacttggttt gtttcatcca tgaaggtaat atccattata catcgcttct
     6301 ttctattctt cttgctattc ttcttgttct tcttttatct tctcaacttc cgatttcatt
     6361 ttaggatatt tgatccgaga atggaaggta ctgcttaaac cgctgatcaa tgatttttct
     6421 actttacgga tttcttttaa cgtcaatcca ctgtcgtcca attgtccatc ggaaatacgc
     6481 tcttcaataa gattatggac aaaactttcg attttttcgc tcgttggatg gtccattgcc
     6541 cgtactgccg cttcacaact gtcagctata ttgacgattc ctgcttcacg gctttgaggt
     6601 tttggtccag gataacggaa ttgctcttcc gtaacttccg ggttgcgttc ctttgcctta
     6661 acgtagaaat aacgcatcag cgtggttcca tgatgctgtc tacaaatatc gatcaccatt
     6721 tgcggcatat tataatcttc caatattttg gcaccatctg tcacatgtcc aaagataata
     6781 tgtttactgt cttctggcaa taagaaattg tgcggatttt ccgctcctga aggcaagttc
     6841 tcaacgaaaa agctggcgtg tttgatcttg ccgatatcat gataatagca agctactcga
     6901 gttagtaaag aacgtccacc gatttctgct acagcattag cacttagatt ggctaccatc
     6961 atgctatggt gatacgtacc tggtgcttct tccagcaact gtttcaataa aggatgattg
     7021 ggattgctga gttcatttaa tacgatcaca ctatcatcat tgaccatcaa ttcaatatag
     7081 gggtgcagac ctatcgtcag caagaatgaa aacagtgcac ctgtgaatcc gcagactagc
     7141 gttaaccaag tcgttgcatc cccaaagctc attccttggt agataatcaa aatgacatcc
     7201 atcatcacag gaaaaataat gacccacata gcagcggata acccttgttc tgagatccgt
     7261 ttgcgtctga cgatcgttgc catgagacca gaaaatacat aggacatcaa aataactgtc
     7321 agaaagttcg tgccaatcga atcataaaaa atgaaaagtg cggatactgc ttgaaaaata
     7381 ccagccatga tccctgctct tcgattcaag aaaaagctta aaacaagtgg agcaaaagcc
     7441 gcggggtaaa acagcggaat ataagcagct tgttctgttt ggaagagttg gaaaaatttc
     7501 attagtacga cgcttactga cattgccgtc acgtagaaaa gtacgtattt tgttctttct
     7561 cctgcatttt ggaattgaag tgaattataa atcagtacac cgatttgaag caaaacagct
     7621 aaaaccatgg caatgagtgg gaaaatagaa gtcgtttgat tcgtcaatcc tagtaattcc
     7681 aattttttca tcgctgaagc atcaatttga tttccttcgc gaacaataat ttctccttgg
     7741 aagatcatca ctggttgtac agcttctcgt gcagattgtt ttaattcttc tgtctttttc
     7801 tcattcaata catcattaac tacgatgcct tgatccacta agtagcgaat catttgttgc
     7861 tgctctgttg tgacattcaa atattgaatc tgatcttctg cttcttgttt aaaagcttcc
     7921 aattcgcttt cacgaacttg tttactcatc tgttcgtcaa tgagcttcaa actttcatcc
     7981 ctaactgtgt gaatttccgt ttcagtcatt tcaaatatcg tcgaataaaa attgttcggc
     8041 agtttttggt aaaacgcaac gttttctgca ttcacgtttt caaaattttt cttcagtgta
     8101 gcaatccgct cttctgtagt aggtttcggg atattttcat cttccttcgc cttatctacc
     8161 ttgccattat aagatttgtt gatggtatca ttcgtcttat cgatcaattc gaataattgc
     8221 ttgatccgat tgttctggtc atctgccaga tctttttgat aagtatattc tggagtcact
     8281 gcttctgctg ccagttttct tctttgttcg gtttcttccg tgttctcaat cgttttattc
     8341 gcacggatac tctcttctgc tacttggcct tctctgaaat caatggcctt ttgtttgaca
     8401 ctactaaatg taacgccgca gacaatgata aaaaacaaga acagaaggaa aggcaatgct
     8461 gcctttccga gtttttcttg tagtttctgc agtgttttat tcaacatgtg cttatcctcc
     8521 taagcaaagc ttattcttga tgaagatccg ccttttcata agctttgatg atttcggcaa
     8581 ctacagggtg ccgaacaacg tcacttgctt caaaattaac aaaatcgatt tttttgattt
     8641 gctttaatgt tcgttctgca tgaacaagac cacttgtcgt cccacgaggc aagtctatct
     8701 gacttgtatc cccattgaca atcatttttg attgataacc gagacgggtc aagaacattt
     8761 tcatctgagc gactgtcgtg ttttgtgctt cgtccaagat aacgaatgca tcatccaatg
     8821 tccgcccccg catgtaagct aaaggtgcga tttcgatcac gcctcgttcc atcaatcggt
     8881 tcgtatgatc caatccaaaa atttggtata aagcatcata aacaggccgc agataaggat
     8941 ccactttttc tttcagatcc ccgggtagaa agcctaggtt ttcccctgcc tccacagcgg
     9001 gccgcgtcaa aatgattttt tgcacttcgc ctttttttag ggcagcaatt gctaatacta
     9061 cggcaagaaa cgttttccct gttccggctg gccctacacc aaatacgatg tcatgtttgg
     9121 ctaccgattc aacgtatttc ttttgaccgc tgttttttac ccgtataggt tttccattac
     9181 ggtctttgac gatctcttcc tcatacatct caacaaaata atctaatgta cctttttgcc
     9241 ccattttcaa tgccgtcaca acatctggcg tacttacgtg gattcctcgt ttgatcaatt
     9301 cctgtaatgt ccgaatgact gaggcagcca aggaaatctg ggattgctca ccaatgatct
     9361 gaatgatttc tccacgtgta tggatggttg tttccgttgt ttcctcgatc agttttatat
     9421 gcttgtcgtg tgcacctaaa agcatgctga catcatcagc agctgtcaat ctaatctcta
     9481 aagaactaga ctcttccgtc aaaaatagac atcccctttt tttcgattca ttctttccta
     9541 ttttaccata ctcagaagaa actatatagc aaataagaaa aggttgtaaa agagtcgctt
     9601 aaccatacgt accaagaaac gttaaaaaat agccgtttcc attcgctaaa ggtctcgttt
     9661 tgataaaaag aagctgagaa tgtaagttat atgaaagtgg agaacaaaat atttcgtttc
     9721 attcaacaag tattctctag gtatttttcc tgtttcaaaa aaagaaaaca aaatagttaa
     9781 aatattcttg catttcaaaa aaaacaggtg tattattacg tcaacaaata aacatacagc
     9841 aaagcgtcga ggaaaacagt aggctattca acgatttatt cccagtgagt catgtaagtg
     9901 agaaatgaca ataaatcgaa tagacgaaca tcatttccga gtcaatgtcg aaaacgtaga
     9961 tcattgtggc tgcctccatt atcaggctag cggataactt tatcaaaaag cgtccgtgaa
    10021 tgaagaagct tttttaagcg aaaaaaggtg gtaccgttct agaatctaga gcccttttcc
    10081 caagtggaaa agggcttttt tttattttaa ggtggtggtt ttcatggaat ctgatattag
    10141 tggcattact aattgctgca acgtttatat cgtcaatcaa aatgataatt attggaggaa
    10201 aaatttatgg aagaaacaaa aacttattca tggaaacaac tattgatggt cagttcactt
    10261 atcttcggga tgttcttcgg gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg
    10321 gctggcgcaa actgggtcag tgctggcgta ggatttgcta tctcaggtgc tcttttccca
    10381 ttactagcta tcttagctgt agtagtgaca aaaagtgatg gtctgtatga cttagctaaa
    10441 cctgtcggaa aatggtatgc tgccttgttc ttaatccttg ttcatttgac gatcggacca
    10501 tttttcggta caccacggac agctgctact gcttttgaaa tggctgctaa accatttttg
    10561 cctgaaaaat ataattcatt aggtatgttt ttattctctg ctcttttctt tttacttgct
    10621 tatttcctta ctgtccatcc aacacggttg gtcaaatacg tcggaaagta tctaaactcc
    10681 ttgtttcttg cattattagc aatcattttt gtcgtttctt tattccatcc aatgggaaat
    10741 ctaaatcaat ctgctgcaga tacttatcaa acaaacgctt taacaaatgg tgtcctagaa
    10801 ggttataaca cggttgatgc agtagctttg cttgccttaa gtgtcacttt tgtccatgca
    10861 gtccgtgacc ttggtttcaa agataaaaaa gtcacagaat taacggcaaa agctggtagc
    10921 ctgagcgttg cgctagaagt catgatctat tttggacttg ttcttctcgg tgcgatgagt
    10981 ttgaataaga tgtctttatc agaaaatggc ggaactgcac tttctcagat cactaatttt
    11041 tatttaggta acttcggcgc tagcttctta ggagtgatgg tcacattagg tgtcttcact
    11101 accgctatgg gactcgttgt ttcttttgcg caagatttcc ataaactgtt ccctaaagtc
    11161 agttatatga catggctgcg gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt
    11221 ttagacaata tcatccaatg gtctttacca gtattgatgc ttttgtatcc attgtcgctt
    11281 gcgttgatct tgctttcctt aacagcaaaa tttttccaaa agacaccatt tgtctatcaa
    11341 gtaacgatgt tgtttgctgc tgttcctgcc gtattggata tgctggcaaa ttcgcctgct
    11401 ttagtgagtc aacaaagagt tgtagcaagc atgctcgaat tttatcatca tcacgttcct
    11461 tttgctgcat taggacttgg ctggatggtc ccaacgttac ttggttacgc tggcagccta
    11521 ctgttttatt acgcctatcg cctatcaggg tataaacaag aagcaaatga actaccagaa
    11581 gaataaccaa atcaaacaca atttaacata agaaatatta aacaggaaaa aaccagctag
    11641 atgaaagaat cctattcttt cgtctagcta ttttttagtt ctctttttta cgcgcacaaa
    11701 aaaactgcgg acaatgattg tccgcagttt aaatgggctc tataatatct agtgttggtg
    11761 acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttgaaa
    11821 tcctttagaa taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag
    11881 agtttaccaa agagttacta gatattctag acctaaatct tatctttcat gaagatgcct
    11941 ttcgcaaaga gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc
    12001 aaccagagga atgcttccat tgtcactatc aaaataagca aacgatcatt aaat
//
LOCUS       DAZKOM010000092        11918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000092 DAZKOM010000000
VERSION     DAZKOM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.092"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1446
                     /locus_tag="ACG8NF_002266"
     CDS             28..1446
                     /locus_tag="ACG8NF_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0472728.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1517..2986
                     /locus_tag="ACG8NF_002267"
     CDS             1517..2986
                     /locus_tag="ACG8NF_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0472729.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            2996..3463
                     /locus_tag="ACG8NF_002268"
     CDS             2996..3463
                     /locus_tag="ACG8NF_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0472730.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3499..4371
                     /gene="scrK"
                     /locus_tag="ACG8NF_002269"
     CDS             3499..4371
                     /gene="scrK"
                     /locus_tag="ACG8NF_002269"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0472731.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4453..6987
                     /locus_tag="ACG8NF_002270"
     CDS             4453..6987
                     /locus_tag="ACG8NF_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0472732.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            7143..7916
                     /locus_tag="ACG8NF_002271"
     CDS             7143..7916
                     /locus_tag="ACG8NF_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0472733.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            7909..8700
                     /locus_tag="ACG8NF_002272"
     CDS             7909..8700
                     /locus_tag="ACG8NF_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0472734.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            8710..9201
                     /locus_tag="ACG8NF_002273"
     CDS             8710..9201
                     /locus_tag="ACG8NF_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0472735.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            9219..9629
                     /locus_tag="ACG8NF_002274"
     CDS             9219..9629
                     /locus_tag="ACG8NF_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0472736.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            9633..11033
                     /locus_tag="ACG8NF_002275"
     CDS             9633..11033
                     /locus_tag="ACG8NF_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0472737.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            11056..11430
                     /locus_tag="ACG8NF_002276"
     CDS             11056..11430
                     /locus_tag="ACG8NF_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0472738.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         4102 a         1616 c         2388 g         3812 t
ORIGIN      
        1 taaaaacctt ttcactggag gaagaaaatg gagaaattat taattaaaag caataatcat
       61 caactttact accaacctaa ggatgtttgg gtcggagatg ttatgcctta cgggaaagaa
      121 ggaaaattct ttatttatca tcaacgtgat actagaaatc cagttccttt tggagaaccg
      181 tttggctggg ctttagcaac tacgaaagat tttgttcatt ttgaagactt tggggaatct
      241 cttttaagag gggatgacaa tgagagagac cagtttatct acgctggttc tgtatttgag
      301 gcaaatggaa aggttcacgc attttataca gggtataatc gtgaattctt agcgcaggga
      361 aagacatctc aagtgttgct tcatgctgag agtgatgatt ttgttcattg ggaaaaatca
      421 gaaacagcat tggagttaga accacaagaa gggtatgata ttcgaaactg gagagatcca
      481 tttgtcgttt gggatgatga aaaagaagaa tatttgctaa ttttaggggc gcgaaagggt
      541 caggataaac atctacaaac cggacgttta gtggaattta catcaaaaga tttaactaat
      601 tgggaattca aaggtgattt ttgggctccg ggattgtata ccatgtttga aatgccagaa
      661 cttttcaaga ttggtgactg gtggtatctt gtctattcag aatactcaga tggaaataaa
      721 atactctatc gaatgagtca aagtttggaa ggtccttgga ttaagcctaa agatgaagct
      781 tttgatggac gggcttatta tgcaggtaga acagcttatg atggaaaacg tcgagtccta
      841 tttggatggg taccaacaaa agatcaagaa aatgataaaa acaattattt atggggaggt
      901 actttggttc ctcatgaagt ctatcaaaaa gaggattttt cacttggggt aaaacccata
      961 gacgaagtgt ggaatgcatt cataaattta catgatattg ccgatgtaga gatagaaaca
     1021 gcagacacaa gagaagaagt aacgcttgtc aaaaatagtg gaactttgtt taatttagaa
     1081 acaacaattt catttgatga gaatcttagt gacttttcac tgagattata taaaaacctt
     1141 gagacagatg aatcttacga atatcgcttc aatttagatg aggggcgtct tacatttgat
     1201 aaaaaccctt gttacccttg gtttcaatat atggataaag gacttattag acctatatct
     1261 ttaaagccta accaatctta taaactaaaa gtgattgttg atgacaatat aatgacactt
     1321 tatattgatg gaatcgctct taacgctcgt gtacatggtt gttttggtca agcaattact
     1381 attacagcaa caaatggatc aatgactgca ataaatacca agttttcaaa tgaaataaag
     1441 aaataataaa cgtcaatcat gtggcaggtc tgaaaatcaa acatgacctg tcacatttaa
     1501 aagggaggat aaatgaatgg actacaaaaa aattgccgaa gatgtattgg aaaacgttgg
     1561 aggaaaagag aatatctcaa gcgctacaca ctgcattact cgtttacgtt taattcttaa
     1621 agacaaaagc aaagcgaata tggaaatgct tgaggaaata gaaggtgcaa aaggtgttct
     1681 tttcaatagt ggacagttac aagtaatttt cggaacaggt ataattgaaa aggtttacaa
     1741 agagttcgtt gaattgacag gagctaagga agtatccgtt tctgaaatga aagacgaagg
     1801 agtcaataat tcaggaaata gacttcaacg agcttttaaa gttttctcgg atatctttat
     1861 tccgatcatt ccagctttta ttggggcagc aatggtttta ggaattcgtt cgttgattac
     1921 cactcctgga atgtttggaa tggatggctc attcgctgat cacaatgtat tcctaaatga
     1981 tttatccaaa tttctgggta ttattgcaac cacatttgat tatttaccag ttttagttat
     2041 gtattctgca acaaaacgtt ttggcggaaa ccctatttta ggattacttg taggatttgt
     2101 tatgattcat ccaggactag ccaatcggaa tgatttcgtc atgggaacta taaagcctga
     2161 ttattggcat ttatttggat taagcattcc agcagttgcg tttcaagggg gtgtttttcc
     2221 agcaattttg acttcttggt ttttagcaaa agttgagaaa attacaaata aatattctcc
     2281 tcaagtactt gcttacattt tagtcccaac tgtgacaatt gttcttgcca actttgcact
     2341 atttttagtg tttggtccat tggggaattt tatcggtaat gttttaggtg gaatcattga
     2401 tttcctatat atgaagatgg gtgcctttgg tgcttttgtt tttgcaggcg cgttacaacc
     2461 attagttgta acaggaacac aacatgctat tcaaggtatt gaggcaaact tagtcgcaac
     2521 aactggattc aattatatcc aacctatttg gtcagtttca attattgccc aaggtggcgg
     2581 ttgtattggt atgtatttac tctttaagaa aaaatctaaa gatagagata ttgcgatttc
     2641 aagctttatt ccaacactgg taggtattac tgaaccggct atctttgctg ttaatttgaa
     2701 atattcaatc gttccttttg tttgctcgtt tattggtgct ggttttggtg gagcatttat
     2761 gaaaattttt gatgtaaaag ccattggaca aggattgact gtattgccag gtctgactat
     2821 tgctaatcca gttcttcctt atatatttgg taatctcata gcattcattt taccaatcat
     2881 ttttattctc gcttatgata aatataaagt gtttatccct gaagctaaac accgtaaatt
     2941 tgcagaaaaa actgactcaa aaaaattgcc agttcaagta aagtgagatg agagcatgtt
     3001 aaattttttt agaaaaaatg aaataagcgt tgtagcacct gcaaccggtg tttttgaaaa
     3061 actggagaaa ttaagtgatc cagtcttttc aaaaggaatg atgggacaag gatttgcaat
     3121 tgatgcaaaa aacgaaacga ttgtatctcc aatcgatggt tttgtttcaa gtatttttcc
     3181 tacaaaacat gccttaggaa taaaaacaaa atcaggaatt gagatacttg ttcatgtagg
     3241 aatagataca gttgagctaa atggtgaggg atttgatatt aaagttcaag aaggacaaaa
     3301 agttaaggct ggggatattc ttatgaccat taattttgta gtttttgaaa agaatcacaa
     3361 gactaaagat gtcattgttg tttttccaga ctaccaagac gaaattttca ttccgaaact
     3421 tgaagaagta aaacaaggac aagaattgtt tgttcttggc taatgataag taattaataa
     3481 tagaatcgag gaaaatgtat ggtttttggg gctattgaag ctggtggaac aaaatttgtt
     3541 tgtgccattg gagataaaga tttgaacata tttgaaaaag tcagctttcc cacgactaca
     3601 cccaaagaaa caatgaattt agttttagat ttttttaatc atcataaaga tgatttagaa
     3661 ggtatcggag tgggatcgtt cggtcctatt gatattcatt gtgaatctaa aacgtatggc
     3721 ttcattacat caacgccaaa acttgcttgg caggattttg attttattgg aagtttaaaa
     3781 aagcaactta atattccaac tgcttggacg actgatgtaa atgcctcttg ctatggtgaa
     3841 tatgtttctg gaaatggcag aggcctttca agtgttgttt actataccgt aggaacgggc
     3901 ataggaggtg gcgctatcca aaacggaaac ttcattgaag gttttagtca tccagaaatg
     3961 ggacatatgc ttgtaaaacg acacaataaa gacaactttt ctgggacatg tccattccac
     4021 ggagactgtt tagagggctt agctgctggt ccggcaatcg aaagaagatc agggaaaaag
     4081 ggacaagaag tgactgaaaa tgacgttact tgggaaatag aagcttatta cattgctcag
     4141 tgtgcatata atacaacatt gatgttatca cctgatgtca ttatatttgg tggcggagta
     4201 atgaaacaaa aacacttaaa aaagaaagta caagataagt ttattgagtt gatcaacgga
     4261 tatgtcgcta ttcccgatgt tgacaattat attatgacac caaaattgtt agataattct
     4321 ggtattgtag gttgtttagc tttagcaaga gacgttaaag aaaaatctta atttggaaaa
     4381 aagatcgtct cgactatttg tttggttgat gcgatctttt ttttcaatta gtaattgaag
     4441 ggacgcctaa aagtgaaaaa aaaaatgaaa gatgaagtat tagagtatat caaaaataat
     4501 ttaaagtatt acgatttttc tgctcaagat attgcaatga aattttgtat taaaagaaat
     4561 gtcgcttccc actatttgaa tcagttattt tcagatggaa aattactaaa gaatgattct
     4621 gttagaccag taatgttcaa gtataatcaa caaaagccaa aagactgctt ttctaagttt
     4681 attggagcag acatatcttt gaaatccact atagataaat gcaaagctac tgtaatgtat
     4741 ccccctaatg gcctcccatt aataataaaa ggaaacagtg gcgttggaaa aagtttttta
     4801 gcaagtttga tctatcagta tgctcttgat agaaaagtaa ttcataatga cgcaaaattt
     4861 gttgttgtga attgtgcaga ttatgcaaat aatccagagc tattgtcagc agttttattt
     4921 ggatataaaa aaggagcctt tactggggct gaaaaagata ctgcagggat gctatcttct
     4981 gctaatggag gatacctttt tttagatgag gttcacaatc taagtgcaga aaaccaagag
     5041 aaattgtttc ttcttatgga ttctcaaaag tatcgtaaac ttggtgaaag tgtgcaatgg
     5101 gagtatgcaa atgttcgcct tatcttagcc acaacagaag ataaaaatac ctctttgtta
     5161 gcaactttta gaagacgcat cccagctgaa atcactctgc cagattacaa taatagaagt
     5221 acatctgaaa aaattcaatt attatttgaa tttcttaaag atgaagcaat tacaattgat
     5281 tcaaaaatat tttgttcgat tgaacttttt actgatcttt tatcgaaaac atttgaagga
     5341 aatgttggag aattgaggaa tgaaatcaag cttctttgtg cagaaggtta tttaggaaat
     5401 aagagaaagt cttcaattta tttaggggac aaactagaca gcggtttttg gatagatcca
     5461 gaaattagta tagatttaaa aaaaatgttt cttaaatcac tttctgaaat tgatttgatt
     5521 gaactttttc aaaatcacat ttcaattgaa gaaagcatga gtcaattaag agatagaata
     5581 cttaaagagt gtgcaggttc ttctgagtat aattatttag aaagtgacga atttatagca
     5641 ttaagaaact atgttgttaa taaaatatca ccaattattg attcgacagg tctatgttta
     5701 ttagatgaat tcttggcgga tatcagtcta tttataatgt ttattgatgt aattgaagaa
     5761 acatcaagaa attttttgct gtctaagttc agaaaatttc gtatgaaaaa tgataaacaa
     5821 aaattattgg caaatgagat ttggagtagt ttagaagtcg aagaaagtaa acaagaactt
     5881 ttgttgaaat ggatactttt tcttgtaaag aagttctatg tgaaaatacc agaaacccat
     5941 tgtctgatag ttatgcacgg taaaataaca gcttctgcga tcgcaaggga aacaaataag
     6001 ttgctgaata catatgttta tgaggctttc gatatgccaa ttgaaggtga gactagtgac
     6061 ttaattaacc taatcaacaa tttttgtaaa agtatagata cgacgaatgg actcattctg
     6121 ctagttgata tgggatcatt ggaacaaatg tatgaaaaaa ttgaatccaa tgtaataggt
     6181 gatttggtaa ttttaaacaa tgtttctaca gctttagcga ttgaatgtgg gattcaagtt
     6241 tgccaaaaaa agccaattag tcatttttac cagatggatt ttgattcgtt tcaagttaaa
     6301 gttcagtatt ataaaggatt atcacaaaag aaaaatgtaa tagtatcttg tctctctggt
     6361 gaggggattt ctgaaaaagt taaggatatt ttaaaacggt atctcgataa tcaagtggag
     6421 attttgattt ttgattttaa cgcattaaag aaaatagcaa aggaaaaaga tactatgatt
     6481 tttaagaata caatttgtgt tctttcaacc actgaatttt acattcaagg aatcgattgt
     6541 ttaaatttag aaaatctaat taatgggaat caaaccttag aaaaattaaa taagtatatg
     6601 gactcggata gctgtgagtt ctgcttaaat gaattagtta aactctttac tattgaaggc
     6661 gctagtacta agcttcgctt tttagattcc aaaaaagtat tcaatgagat agaaaaagta
     6721 ctatatttgt atgaaaaata ttacgatgtc aaaatcccaa gttttctgag aattaatcta
     6781 tttctacatc tttctggaat gattgaacga attatgattg gtgatggaat aacaaatgaa
     6841 catttgaaag agggagcgca actatttgat acttttttaa atgtatcaaa agattttttt
     6901 tcagaaatca aagatattta ctgtattgaa atacccaaag gagagtatga gttaatctat
     6961 acaatttttg agcagactat tttctaaata gcatactagc gtctaaaaaa atgaaagggt
     7021 gtccaaagta tatttagacg ctcatttaat taatatttac tgtattttcc cgctttattg
     7081 gataatgtta gttggcacag taattgctta tataaaagta taagaagaaa ggagagaaca
     7141 aaatggcgtt ttggcaaaca tttttagttg tgttgtatgg atttttcatt aactgggata
     7201 aggaagggcc caaaatcgga acctcccaac ctgtcgttgc aggtttcttg accggactta
     7261 ttatgggaga tattcaaact ggactttata tcggtgggac tttgcaatta atgacactag
     7321 gtattactag ttttggtggt gcatcagtac cggattatca aacagcagca ttgatcggta
     7381 cttatttagc aatctcaact aaccaggatg cttctatagg aataacttta gcaataccgg
     7441 tagcaatgtt aattgtacaa ctagatgttc taaagtggtc aacaaacatt ttttttcaac
     7501 aaaaggcaga aaaattcgca caaaaagaag attatcaaaa attagagtgg atgcaatatt
     7561 tggcatcaat taatacaagc tttgtatctg gaattcctgt tttgttaact gtgatattag
     7621 gcccaacgtt agtgggaagt gcaatcaatt atattcctga atgggttacc ggtggtttaa
     7681 aggtagcagg gggactatta cctgctgttg ggataggaat gctacttaaa tatttaccta
     7741 caaaagatta ctttgcctat ttgttagttg gtttcgtttt ggcagtttat ttccaaatac
     7801 caattttagg gatttcgtta cttggcgctg caatcgcact aatcgtatat aaaaatagat
     7861 caaataatac tcaagtagca ccagctgcaa caggaggttt tgacgaagat gaataagaaa
     7921 ctgacaaaaa tcgacaaaca agtaattttc cggtggattc tcaatggtgc aacttcatta
     7981 aattatgaaa aaatggaagg gttgggttat gcgtattcta tgcttccttt tatcaaggac
     8041 acatataaag atgatcctga aggaatgaaa caatccgttt tagctcacct acaatttttt
     8101 aacactacgc cgtatacagc gccttacatt attggtatga atgttggaat tgaaaaagaa
     8161 gaaaaaaata agtctttgga agcagtaagt gctctgaaaa caggtctaat gggtccttta
     8221 gcgggaattg gagatagtgt atttgtagtt attccatgga ctatttttgg agcaattgca
     8281 gctaatatgg cattggaagg tagttttgtt ggaattattt tgtggatact ggcatcaatt
     8341 ttattaagga ttactgctta tcctttatac cttgctggga aagaatcggg tgcgagccta
     8401 ctaactcaaa ttgaaacaaa attaaagtct attactgatg cagtatcaat tttagggtta
     8461 atggtggtcg gtgcactcat tgccacagtt gtaaaagtga atgttggctt tgaatttaaa
     8521 caaggcgact taacaatgac cggaaatgaa atctttgatc aagttatgcc tggtttatta
     8581 cctgcatgtg tagtagcaat tgtgtattat ttgttaggac gaaaggttaa accagtttat
     8641 gtaattctat taatcttagt agtttctatt gcgttaagag cgttaaatat tttgaagtag
     8701 gtgttgaata tggatgaaaa aatgaaaggt attatacatg ttcgtattga tgatcgaatg
     8761 attcatggac aagtagcaac acaatggaca aatcaattga gtgctaacag aattatggtt
     8821 attaataatg aagtagccaa tgatgacgta aaaaaagctg tagttcgcct cgctgcacca
     8881 cctaatgtta gaacttcaat aattacaagg gaagtagcag ttaaaaatat tctttctggt
     8941 aaatatgaag gacaaaaagt tcttattgtt gcagtttctc caatcgatgt taaatttttg
     9001 attgataacg gtttaccaat aacaagtgta aacgtaggaa atttatcaag aaggaatgga
     9061 acagaacgca tccgtccgac gataaatatt actgaagaag agagagaggc ttttaaagaa
     9121 ctaattagtg atggagttga agtgacagtg atacaaactc ctagagattc aaaagaattt
     9181 ttaaaggatt ttatttatta agaacagagg atgagtggat ggagtttata attacatctc
     9241 atggaaatat tgccattgga tttgtagata cattaaaaat gttttttggc gataatttaa
     9301 attgtcatgt ggtaacactt ggagatgcag gcattgaaga attccgtgaa aacattgaaa
     9361 aaatagtttc tcaagtaaaa aatcaagaag ttatggtatt tgctgacttg attggtggaa
     9421 cacctttcaa cgaatttgct aaaagaagtg aagtatttca aaatgggttt caacttcttg
     9481 gtggcatgaa tttaggaatt ttgattgagg ctgtcaattt gagattacaa ggaaaaaaaa
     9541 gcgatgatat catcgaaaat ctaagaaata tgaatacaat tgcgtgtctt gcagatatgc
     9601 aaagacatac taacgaagag gatgagtgat aaatgcaaaa aattttttac aagcctaaag
     9661 atttttggtt tggggattgc atgccttttt ataaagatgg aactttttat ctatttcatc
     9721 aacgagatac gagaaatcct aaacctttaa ctgatccttt tgggtggtca ctagttacaa
     9781 ccactgattt tgtgaaattt gaagaatacg gggaagtaat tcatcgtggt ggcaacgatg
     9841 acattgatca atacatttac gctggaagta ttgtccaagg aatcaatgag tatttagctt
     9901 tgtatactgg acataatcgc aaagctaagt tggatggaaa aacttcagaa gtgttaatga
     9961 ttgcaagttc taaagattta attcattgga aaaaggatgg cgaagcagca aaacttgttc
    10021 ctcaagaagg atatgataag aatgattggc gcgatccttt tgcaattttt gatcaagatc
    10081 atggaaaata cgttttgata ctaggagcta gaaaaccagc aagtaagaaa aagccgacgg
    10141 ggagattggt ctattttgaa tccgaagatt taaaagattg gaatttcaaa ggagactact
    10201 gggctaataa tgaatttaac atgattgaaa tgcctgatat atttgaaatt gatggaacgt
    10261 ggtatttact tttttcagag tattgtgaag ataaaaagac taaatatcga gtcggagcag
    10321 gattattttc agattggaaa tcaccagtag acgaagcttt cgacggaaaa gcctattatg
    10381 cagcaagaac agtcggaact aatgaaactg gccgttttct aactggctgg gtggcaacaa
    10441 aagaagggga gaacgatcaa aataactggg attggggcgg tgcacttgtt ccacatcaaa
    10501 ttattcaaaa gacagacaaa actcttggtg ttaaactgcc tgaaaaaata attgagaatt
    10561 ttaaagaaat cactccattt tcaagtttta agttagagaa agaatttgga aaaaaagaaa
    10621 gagtaattgt tcctaaaatc gaagattatt ttctatttga aacaactttg actattacat
    10681 cgttaacaaa ggagtttgct ttaaaattat ttgtagatag tgatactcaa gaaggatacg
    10741 aatttcgtat ttcattgatt aataatcagc ttactttcgg aaaaactccg aactatcctt
    10801 ggcctcaaat gtttgataag gggctgcaac gaccactatt tttggaaata ggcaaagaat
    10861 atgaactaaa gttaattgtt gatgatacga tagctgtttt atatattgat aatgttgctt
    10921 taagtactag aatgtatgat aaagtcggaa cagcattaag tatgactgtt acggagggag
    10981 caatcaaggt agataaacta gactattcaa taaattatga atgtggattt taaaacaatt
    11041 gaagggatga tgtaagtgaa attaaatggt ttacaacata ttggaattcc aacaaaagat
    11101 tttggagctt caaaaatctt ttatgaatcc ttagggttta gtctgattaa tgaagaaatg
    11161 aatgtgggaa gtaaagtcgg ctttttcgag ttgaatggaa cgataataga aatatgggaa
    11221 gataaaacta ctgagtgtag aggagccatt gatcatctag cattagatac ggatgacata
    11281 gatgaggtat tcaattcaat tcaagattta ggatatgaat tattagataa aaatattatg
    11341 gaattaccgt tctgggaaaa tggaatccgt tactttaatt tctacggacc aaatcatgag
    11401 attatagaga taagccaaat gaagaaataa atagataacg aagtgctcta agttggtatg
    11461 aacgaaaagt acttcatttt ttggtatcag catttcaata gtttaatgag aatttactat
    11521 ttcaaattaa ttcaaagtga cagtattttt tacttatttg gtttcaaatt ttgtaatcct
    11581 tcctatttat tggaatacac tccacatttt gtatacaagt catatcatgt tttagcaaag
    11641 ctcttaccaa gcaaagttat aatgatgttt tccaaagttt gatctttcga tggaggctaa
    11701 cacatatttt ttgcaggaca gccaaaatta gatgtataga atagagtctg tgaggcgtat
    11761 agacagttta ttgtctgtat gccttttttt attttgaaaa atttttagta tttttcgaaa
    11821 aatgctcact cctagagtaa gcgccccata gaacagtacc ttactaacaa atgcacctca
    11881 agatatactt gagctatcaa gaagtctgga ggttttct
//
LOCUS       DAZKOM010000093        11731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000093 DAZKOM010000000
VERSION     DAZKOM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.093"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACG8NF_002277"
     CDS             complement(364..1482)
                     /locus_tag="ACG8NF_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472739.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACG8NF_002278"
     CDS             1715..1837
                     /locus_tag="ACG8NF_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472740.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACG8NF_002279"
     CDS             1953..3260
                     /locus_tag="ACG8NF_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0472741.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACG8NF_002280"
     CDS             3379..4578
                     /locus_tag="ACG8NF_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0472742.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACG8NF_002281"
     CDS             complement(4605..5873)
                     /locus_tag="ACG8NF_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0472743.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACG8NF_002282"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACG8NF_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACG8NF_002283"
     CDS             complement(7090..7593)
                     /locus_tag="ACG8NF_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0472744.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACG8NF_002284"
     CDS             complement(7716..8480)
                     /locus_tag="ACG8NF_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0472745.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACG8NF_002285"
     CDS             8588..8863
                     /locus_tag="ACG8NF_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0472746.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8NF_002286"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8NF_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0472747.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACG8NF_002287"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACG8NF_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11435..>11731
                     /locus_tag="ACG8NF_002288"
     CDS             11435..>11731
                     /locus_tag="ACG8NF_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472748.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3560 a         2450 c         2076 g         3645 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggcaa
    11581 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    11641 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    11701 gcctgacgtc attatcgggc gaaaagaaag g
//
LOCUS       DAZKOM010000094        11527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000094 DAZKOM010000000
VERSION     DAZKOM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.094"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..707)
                     /locus_tag="ACG8NF_002289"
     CDS             complement(222..707)
                     /locus_tag="ACG8NF_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0472749.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    797..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..1828
                     /locus_tag="ACG8NF_002290"
     CDS             1187..1828
                     /locus_tag="ACG8NF_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0472750.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1847..2482
                     /locus_tag="ACG8NF_002291"
     CDS             1847..2482
                     /locus_tag="ACG8NF_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472751.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2500..3315
                     /locus_tag="ACG8NF_002292"
     CDS             2500..3315
                     /locus_tag="ACG8NF_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0472752.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3634..5070
                     /locus_tag="ACG8NF_002293"
     CDS             3634..5070
                     /locus_tag="ACG8NF_002293"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0472753.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5259..7706
                     /locus_tag="ACG8NF_002294"
     CDS             5259..7706
                     /locus_tag="ACG8NF_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0472754.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7936..9003
                     /gene="rlmN"
                     /locus_tag="ACG8NF_002295"
     CDS             7936..9003
                     /gene="rlmN"
                     /locus_tag="ACG8NF_002295"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0472755.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8993..9199
                     /locus_tag="ACG8NF_002296"
     CDS             8993..9199
                     /locus_tag="ACG8NF_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472756.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9196..10299)
                     /locus_tag="ACG8NF_002297"
     CDS             complement(9196..10299)
                     /locus_tag="ACG8NF_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0472757.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10852..11400
                     /gene="aac(6')"
                     /locus_tag="ACG8NF_002298"
     CDS             10852..11400
                     /gene="aac(6')"
                     /locus_tag="ACG8NF_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0472758.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3826 a         1949 c         2483 g         3269 t
ORIGIN      
        1 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
       61 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
      121 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
      181 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
      241 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
      301 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
      361 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
      421 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
      481 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
      541 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
      601 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
      661 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
      721 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
      781 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
      841 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
      901 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
      961 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     1021 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     1081 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     1141 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     1201 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     1261 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     1321 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     1381 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     1441 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     1501 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     1561 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     1621 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     1681 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     1741 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
     1801 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     1861 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     1921 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     1981 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     2041 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     2101 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     2161 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     2221 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagctat
     2281 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     2341 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     2401 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     2461 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     2521 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     2581 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     2641 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     2701 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     2761 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     2821 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     2881 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     2941 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     3001 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     3061 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     3121 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     3181 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     3241 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     3301 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     3361 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     3421 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     3481 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     3541 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     3601 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     3661 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     3721 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     3781 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     3841 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     3901 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     3961 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     4021 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     4081 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     4141 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     4201 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     4261 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     4321 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     4381 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     4441 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     4501 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     4561 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     4621 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     4681 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     4741 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     4801 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     4861 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     4921 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     4981 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     5041 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     5101 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     5161 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     5221 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     5281 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     5341 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     5401 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     5461 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     5521 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     5581 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     5641 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     5701 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     5761 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     5821 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     5881 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     5941 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     6001 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     6061 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     6121 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     6181 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     6241 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     6301 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     6361 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     6421 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     6481 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     6541 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     6601 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     6661 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     6721 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     6781 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     6841 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     6901 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     6961 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     7021 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     7081 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     7141 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     7201 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     7261 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     7321 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     7381 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     7441 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     7501 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     7561 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     7621 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     7681 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     7741 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     7801 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
     7861 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
     7921 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
     7981 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
     8041 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
     8101 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
     8161 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
     8221 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
     8281 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
     8341 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
     8401 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     8461 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     8521 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     8581 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     8641 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     8701 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     8761 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     8821 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     8881 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     8941 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     9001 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     9061 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     9121 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     9181 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     9241 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     9301 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     9361 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     9421 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     9481 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     9541 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     9601 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     9661 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     9721 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     9781 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     9841 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     9901 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     9961 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    10021 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    10081 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    10141 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    10201 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    10261 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    10321 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    10381 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    10441 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    10501 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    10561 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    10621 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    10681 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    10741 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    10801 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    10861 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    10921 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    10981 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    11041 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    11101 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    11161 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    11221 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    11281 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    11341 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    11401 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    11461 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    11521 gtgtacc
//
LOCUS       DAZKOM010000095        11193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000095 DAZKOM010000000
VERSION     DAZKOM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.095"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..893)
                     /gene="ftsX"
                     /locus_tag="ACG8NF_002299"
     CDS             complement(9..893)
                     /gene="ftsX"
                     /locus_tag="ACG8NF_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0472759.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="ACG8NF_002300"
     CDS             complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="ACG8NF_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0472760.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1677..2778)
                     /gene="prfB"
                     /locus_tag="ACG8NF_002301"
     CDS             complement(join(1677..2705,2707..2778))
                     /gene="prfB"
                     /locus_tag="ACG8NF_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0472761.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2904..5438)
                     /gene="secA"
                     /locus_tag="ACG8NF_002302"
     CDS             complement(2904..5438)
                     /gene="secA"
                     /locus_tag="ACG8NF_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0472762.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="ACG8NF_002303"
     CDS             complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="ACG8NF_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0472763.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6339..7058)
                     /locus_tag="ACG8NF_002304"
     CDS             complement(6339..7058)
                     /locus_tag="ACG8NF_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0472764.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7055..8368)
                     /locus_tag="ACG8NF_002305"
     CDS             complement(7055..8368)
                     /locus_tag="ACG8NF_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0472765.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8449..8769)
                     /locus_tag="ACG8NF_002306"
     CDS             complement(8449..8769)
                     /locus_tag="ACG8NF_002306"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0472766.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8877..9164)
                     /locus_tag="ACG8NF_002307"
     CDS             complement(8877..9164)
                     /locus_tag="ACG8NF_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472767.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9291..9935
                     /locus_tag="ACG8NF_002308"
     CDS             9291..9935
                     /locus_tag="ACG8NF_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0472768.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9994..10701)
                     /locus_tag="ACG8NF_002309"
     CDS             complement(9994..10701)
                     /locus_tag="ACG8NF_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0472769.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10921..11091)
                     /locus_tag="ACG8NF_002310"
     CDS             complement(10921..11091)
                     /locus_tag="ACG8NF_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472770.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3051 a         2430 c         1956 g         3756 t
ORIGIN      
        1 ttgtggtatt agaatttcaa aaaacgtctc atagaaatga tcgagccaag tgaaccaatg
       61 atcacaccaa tgccgaccat taaaagattg attttccaaa cgatattttg tggacgaatc
      121 aatgaataat gggaacgtaa taatgatgga ttgatcaatt ggaaaaacca tgcataacca
      181 aatgtgatga tcaaaatcgg aagaatcgca ccgatcagac cgatccagcc accttcaagg
      241 aaaaacggcc agcgaatata tccatttttt gcaccgacta aacgcatgat ttgaatttca
      301 cgctgtctag acaaaatcgt gatccgaatc gtgttcgaaa tgagaaacat cgctacgaac
      361 agcagcaatg ccgctgcaac taatccccat gtccgaactg caccagcgat ttggaaaatc
      421 cgatctgagg aagctccacc gtaatcagaa cgagaaacat tcttcaaatc tgctgcttct
      481 tctgctactt gttttgtata agaaggatca gtagcactta ctacatacac atcatataac
      541 gggttattgt caccttcaaa caatttccag gaatcaccca tttcgttttg gatttttttc
      601 aattcctgtt ctttgcttga aaattcaact tttttaacat gatctaagtc attcaattct
      661 ttttctagct ttttcatctc ttcgtctttt gtaccgatat ctacgaatac agatacatca
      721 acgtttcctt ctatatcctg agctaattta gttgcattca tgataactgc catgaataca
      781 ccgactaaag tcaacgtgat cgttaccgca ctgactgaag caactgtcat ccagccgtta
      841 cgtttcaaac tcttgatact ttctaataaa tggcggaaaa aggttctaat catcgtatcc
      901 atattcccct tccgcttgat ctcgaataat gcggccgttt tcgatagcaa ttactcggtg
      961 ccgaatcgta tttacgatcg tactgttatg tgtagccatc acgacagtag ttccttgggc
     1021 attgatccga tctaataatt tcatgatttc ccatgagttt tctggatcca gatttcctgt
     1081 cggttcatcc gcgataagta ctttaggcgt attcacgatt gcccgagcaa tagagacacg
     1141 ttgctgttcc ccaccggata attcgctagg aaagactcga actttgtgtt tcaaaccaac
     1201 aaggtccagc acttccatta ctcgtttttt gatatctcga ggttttctgc cgataacttg
     1261 catagcgtac gcaacatttt catatactgt ttttcttggt aataatttat agtcttggaa
     1321 caccacgccg atttctctgc gtagataagg aacttcgcgg tttttgattg ctattagatc
     1381 atgacctgcg acgttcaaag ttccttttga agctttttct tctcgataca tcaatttgat
     1441 gaatgtagat ttaccggctc cagacggacc aaccacatag acaaactctc cttgttcgat
     1501 gacaatcgag agattgcgga tagcggtcgt accatttgag tacttcttca ttacatcctt
     1561 catttcaatc atggcgttct ctccatatct ttttcagtct ttagctatta tagcatggca
     1621 atgtttcaac atgcttgcaa aatcttacaa ttttatttca atttaacaat gagttcttat
     1681 tgtagatgca tttttaaata gccgtcaata aataaatcaa ggtcaccgtc cataactgct
     1741 tggacatttc ctgtctctac gtttgtccga tggtctttta ccatagaata gggatggaaa
     1801 acataagaac ggatctgtga accccagccg atttcttttt gttctcctcg aagtgcagct
     1861 gcttcttgtt ctttcttttc cacttctaat tgataaagct tagcttttaa catccccatc
     1921 gcttgctcac ggtttttcaa ttgtgagcgt tgtgcctgac tagctactac tgttcctgta
     1981 ggcaagtgag tgatccgtac agcagattct gttttattga tgtgctgacc acctgcacca
     2041 cttgctcggt acgtatcgat tttcaaatca tctggattga tttctatatc gatcgtttca
     2101 tcaagctctg gcatcacatc taccgaacaa aaagaagtat gacgtcgttt cgctgagtca
     2161 aaaggcgaaa tccgtacaag gcgatgaaca cctttttctg attttagata accaaaagcg
     2221 ttatgtcctt taatcagcag ggtcacactc ttgatccctg cttcatcacc tgcttgataa
     2281 tctagtgttt ctacttgata gccatgctgc tctgcccaac gtgtatacat acgtaaaagc
     2341 atgctgcccc agtcttggga ctccgttccg ccagcccctg gatgcaattc gacgatcgca
     2401 ttgtttcgat cataaggtcc gtctaacagc atagataatt catacgtctt cattttttca
     2461 tctaatgcat tgatacgtgt ttctgcttct tcttgcattg ctgcatcagg ttcttcttgc
     2521 agcatctcca ccatcaattc gatttcttct agttcttcgg caagagaatg gaattggtca
     2581 taggtttctt tattagcgtt attttcgctg atcacttttt gtgcggcttc gctgtcatcc
     2641 caaaaaccag gttcagccat ttttgcttct gcttccgcga tgtcttcttc cagctgatct
     2701 aagtcaaaga gacctcctga aacttgtgac cttttcctgc atttctgtca ggagatttcg
     2761 aatttcggat atttccatgt tttttccttc tttcactata cttttttcat tatatctagt
     2821 ctaacagaat cagcaaataa cagaaagaag gtgagacgaa ttcttaaaaa aagaatttgt
     2881 ctcaccaatc ctgctgtcag aaattagttt gcattttttc catgacagtt cttatatttt
     2941 ttcccgcttc cacaaggaca aggatcattt cttccgacct tgttcacatg gattggctgt
     3001 tttttcgcgc tagtattact attttctttg gcatctccgt cttctgagtg agaagctgtt
     3061 ccttgagaaa cctgttcacg ctgtacgttt tgacggatct cagctttcat aaagattcga
     3121 gtgacttcat actcgatggc gcctaccata tcttcaaaca tcttatagcc ttctgtttga
     3181 tattcgacta atggattgtt ctgtccataa gcacgtaagc cgattgactg acgtaattga
     3241 tccattgcat caatatggtc tgtccattta gagtccacaa cacgcaagat cacgactttt
     3301 tcaaattcca acagttgctc tggaccattt agttgagccg ctttggtatt aaatacttct
     3361 tgtgctcgtt cgtttagata ctctttgatc tcctgagatg tttttccttc gatatctgca
     3421 agagtaatac tatcttcatg gacaagagta ctgccggcaa aatcgatgat actttccaaa
     3481 ttccaatttt ctttctctaa ttgtgtatgc gcatctacta cacgactgat tgtccgtttg
     3541 accatgttca ttagtggctt ggataagctt ttttcttcca tgattacttc ttgtcgctgt
     3601 ttatagatta cttcacgttg ttcacgcatc acatcatcgt attgtaagac gtttttacgt
     3661 gtgtcatagt tatttccttc tacacgtttt tgtgcagatt ctacttgttt cgtcagcatc
     3721 ttgctttgga taacagcatc ttcctcaccg atcttcatcc gatctaagaa agctttgatt
     3781 cgttctgaac cgaatcgttt catcaaatca tcttccaatg acaagtagaa ttgtgacata
     3841 ccagggtctc cttgacgtcc tgcacgtcct cgaagctggt tatcgatacg acgagattca
     3901 tgtcgttctg tcccgataac tgctagacca ccaagttctc ttaccccaag tcctaacttg
     3961 atatctgttc cccgaccagc catgttggta gcaatcgtca cagcaccttt ttgaccagca
     4021 ttcatgatga tctctgcttc tttaaagtga tttttcgcat tcagcacttc atgaggaata
     4081 cgttcttgat tcagcatatc tgataataac tcagatgttt caaccgcaac agttccgact
     4141 aacacaggtt gacctttgcg gtagcgatct ttgatatctt gcacgacagc gtggaacttg
     4201 ctttgtaatg ttgggtaaag taaatctgga cgatcatcac ggataaccgg acgatttgtc
     4261 ggaatctggt atacttggat gttataaatt tctctgaatt cttcttcttc tgttttagct
     4321 gtaccagtca ttcccgcaag tttcttgtac atacggaaat agttctggaa agtgatcgtc
     4381 gccatggttt tcgtttcgtc ttcgatctct acgccttctt tagcttcgat tgcttggtga
     4441 agtccatctg aataacggcg tccatccatg atccgaccag tgaactggtc aacaatcagt
     4501 acttttccgt cttggacaac ataatcgata tctaaaagca tgatatagtt ggctctcaat
     4561 gcttgatcca agtgatgggt caatgcagta ttttcaatgt cgtataagtt atccaaaccg
     4621 aatgtttctt ctgctttttc gatccctgct tcggttaaac tgattgtttt tgattgtaca
     4681 tcgattttat aatcttcttc ttctttcaaa cgtttcacga agttgtctgc tcttgtatat
     4741 aaagctgtgg atttttctgc ttgccctgaa atgatcagcg gcgttctcgc ttcatcaatc
     4801 aagattgagt caacttcatc gacaatggca taattcagcg gacgttgtac catttgatgg
     4861 cggtaaacga ccatattgtc tctcaagtag tcaaatccta attcattatt cgtactataa
     4921 gtgatatcac aattataagc ctcgcgtttt tcatccgaag attttgagtt gatattcaac
     4981 cccactgtta atcctaagaa attataaagt tcgcccattt ccgtcgagtc acgggttgct
     5041 aaatattcat tgacggtaac tacgtgtacc ccttcgccag tcagagcatt tagatatact
     5101 ggcatcgttg cggtcaaggt tttaccttca ccagtacgca tttcagggat atttccatcg
     5161 tgaagcacga ttccacccat caattgtacg tggtatggat ataaccccaa tacacgtttt
     5221 gctgcttctc ggacgactgc aaaagcttcc ggaagtaatt gatctaatgt ttctcctttt
     5281 tggtatctgc ccttgaattc agcagttttt gcctgtaatt gttcatcagt taatgctgcc
     5341 atttggtcag cgtatgcttc aacttttttt gcaatgctat ctaaacgttt tagttctttc
     5401 ttatcgtttt cgataagtga acgaatcaaa ttggccatgc gttgtgtaat ctccttttcc
     5461 ttctgtgtat tatccagact atgtagactt tacttttcat tttatcatta gttatagata
     5521 attgaaatat cttaagcgta ttaaagtaag aacttcataa tttattcata tttcatctaa
     5581 aagaggagga aactcaaaaa aacttgcggc agctgtcagt agctgctgca agttttcgtt
     5641 gttatttaag taaggctgat cagtctgttt caatcagtcc gtattttcca tctttacggc
     5701 ggtaaacgat acttgtacca tttgtttctg catcttcaaa aataaagaag ttatgtccca
     5761 gcatgttcat ttgtaagact gcttcttcac tatccattgg tttcaatgaa aggcgtttcg
     5821 tacgtacgat atctaattca gaatcatttt cgttttcgtc ctcttcattg aataaagcag
     5881 cagcatctgc agtattcatg cccgtttcac gggatttacg attaattttt gttttgaatt
     5941 tgcggatttg gcgttccaat ttatcaacaa ccaaatcgat acttgcgtat aaatctggag
     6001 aagtttcttc ggcacgtaat acaagataag gtaatgggat cgttacttcc acttttgctg
     6061 ttttttctgt ataaactttt aagttcacat gcgccgttgc atcgggtgcg tcactgaaat
     6121 agcgttctag tttgcctact tttttctcaa cgtagtcgcg aatcgcttca gttacttcga
     6181 tattttcgcc gcgtacatta tatctaaaca taaaaattac ccctttcgtc taccacaatc
     6241 aagaaggaga aggaccactc tttccctctt cttaattcta ttatatcgaa ctctaggtta
     6301 tttgcaaatt aaatcgctat caaatttcca catctaattc atctcgccaa agaaatggtt
     6361 cggatgattt tcggctgtgc gacctgcaaa caatctgctg cgtggaaaag agtctgtcct
     6421 gttgtataga catcatcaat aatcaatatt tttttatttt gtatcttaga aaaggtgact
     6481 gcaagttcaa aaggttgagg catctccaat ctttcctttc gcgtgttttt tgcttgagcc
     6541 agcagatgct tctctctttt gagtagcatc tgaaaaggaa ttcttgctgc tttcaacatc
     6601 tcgcttactt gattgaatcc tctttctgtt cgcctttttt cagataaagg tattggacaa
     6661 tagatgtatg tcgggtattc tttttttatc tgcgtccatt tatatgcaaa ggtgcctgct
     6721 agtcggatat ctcccataaa cttgtattga ttgagccagg ccttcatatt ttcatcgtac
     6781 acaaaaaagg cacggtgctg aaaatcatag gtcggatatt tcttcttcca ttcttgacag
     6841 tcactgcagt aaacgggaat agactttact ttctttttcg tactctctag attgaaatgg
     6901 attggttttt gacagccaga acagcctctt gtttccaata aatgaaattg tgcgcgacaa
     6961 actgaacaag tctgaggtaa tctgtatttc gaaacaccaa taatctcgct gaatgttaaa
     7021 ttttggaaga tatctttact gcagcaactg cacctcacag catcttcttc ccttcttcat
     7081 tcatttcttt gatttcctga caagcttttc gaatagctgc agttttttct tcatagaaaa
     7141 agtatacttg actgttcgta taaggccctt tacgatcggc tcttcctgcg atttgtacca
     7201 aagaagattt agtaaaaacc ggatgatccg ctcctaaaac gataacggat attctttcaa
     7261 atgtgactcc acgttcaagg atcatcgtgg ttaacagtat atcatatttt gcttctcgca
     7321 ttttctggac tttttccgcc cgctgattgt ctcttgaaga aacatctgtg atctcatatc
     7381 tcggaagagc ctcttgaagt gtggctttca aacgatgcat catcgtgata cttggacaaa
     7441 ataaaaggat atggttttct ttaattaatt gatgaatgat ccgcaccaaa gattttatct
     7501 tcttcttgtt cttgcatttt tgtcgccaat tattcatgaa aaccaagacg ggttcaggaa
     7561 gcaaacgccg atgaaagcgt aaagcaagtt tgtggatgtc aaacgtctgc ttgatttcct
     7621 ttagtaactt tttatcaggt gtggcactta agtagatgaa tcgtccgtct ggtttaatcg
     7681 ctgtttgcac agcatgagct aatcctgcat ccccgttata aggaaacgca tcgatctcat
     7741 cgatcactat taaatcaaag gaccgataaa aacggtatag ctgatgaatc gtacagataa
     7801 caaaagaagt aaaggtataa gccgttgctg tattcccatg gagcaacgtg attttttctt
     7861 ctggaaaaac ataacgatag cgtaaaaaca attcattaca aacatcgact cgcggtgtac
     7921 aaatcgctac tcttcctcca gcttttaaag tttccactaa aataggataa agcatctctg
     7981 ttttccctgc acctgttaca gcccaaataa gatgatgtct tttttctgaa tggcaaagta
     8041 ttctctttga gatttccgtt tgtttttcag taagctgtcc tgtccaaaaa aagacgactt
     8101 ctcgaggttt tgcttcaggc tgatcaaaca aatataaatc ttgttgagtg tcgcatcgcc
     8161 ctaaatatag acaatgtgta caaaaataac cgtaatctgc aagctgtcca tcaatgacgg
     8221 acgtcctcat tccacagcga tgacaaagaa tccagttgcc caaacgttcc actgctgcga
     8281 tcttctcata atttggtaaa tttacctcct cacagtctat tgcttcaata tctctcgtca
     8341 gaagccttct accccaaaaa tgattcatat tcttcgccct aaaaaaatat acgaaaaaac
     8401 aagcgatcta ttttttggat cgcttgttcg atagttatac tgttctattt attcacctat
     8461 gagcgtgatt gcttgatcta acactttttc gccattcatc ataccataat cagccatatt
     8521 gatgacatct aaaggaatgc cttttggttc cagacgtttc tcaaaatcag ctttcataaa
     8581 gcgaacttgt gggcctaata acaacacatc cacgtcttta tttgcaaggt tgttatctgc
     8641 gtcagaagcg gacacagcga aaatatccga gtcgattcct tttgcttcag cagctttttg
     8701 catttttgtt actagtaaac ttgtgctcat accagccgaa catacaagca tgattgtttt
     8761 tttagccata cttttctctc ctttttaaaa caaactatgc aaacgttttc ttcgcgtctg
     8821 ttactctatt atataaaaag aaagaaattt gtcaacgttt tctaaacgcg gtttatttaa
     8881 tcaaatagcc aactagattt cactaattta ttgaacacga catattcttt gtccaaattg
     8941 atgacaacta cttcatagcc tgcatttatc cagccatagg cacctttttc agggtgttta
     9001 taatcatttg cccaccaatc tgtactttcc cttgctgttt tgggaagtcc gttactggga
     9061 aacagcagtt cgtcaaattg ggtaaaggtc aacgttactt gagagccgcc attgttcttc
     9121 aaataatgcg taattgcatc gtactttttc attcgttcct gcatccccag atccacctgc
     9181 ctttgtcttt attatcgcat actacagcaa ttgcgcaacc gttacagtgt tgaaacaacc
     9241 tagaacttta tttatactga ataaagaaaa tgaaaaaaag aggtgacaag atgatcgagc
     9301 gctatcgtac aataaaagaa aatggacaaa gtgagattga gatcaaaaaa tcacgcttta
     9361 tctgttctct tagacgaata gaatcagagg accaagcaaa aagtttcatt caagaaataa
     9421 aaaaagaaca ttggaaagcg aaccataact gcagcgcctt catcattgga gaaaaaaatg
     9481 aaatccagcg aagcagcgat gatggagaac caagtggaac ggcgggcgta ccgatgttgg
     9541 aagtcctcaa aaaaaatcaa ctgatcaatg tagctgcagt agtcactcga tattttggag
     9601 gaacgaaact cggcacaggc ggattaatac gagcatacac tcatgcggtc tctcaagcat
     9661 tgtctgacat tggaatcgta gaaggtacct tgcaacagga gatctctgtg atgatcacgt
     9721 atccgcaact aggaaaatta cagaattatc ttgaactcca acattttttt ataaaagaaa
     9781 tccaatatac tgatcaagtt agcgtcatct gtgctgtaga tgaaaaaagt gctgaccact
     9841 ttaaagaatc catcattgag ctgctgaacg gacaagtcca atttgaagag ggttctgttt
     9901 cttatcacga gcaactgata actgatcata tataaaaaca ggtgattctg attctctttt
     9961 atttctaata aggaatcagg gatcacctgt tttttatttt tccaaataaa actcaaaacg
    10021 atttcctacg tattgcgtac gcacatactc aaacggcgta ccgtcttcaa aataagaaat
    10081 ctggcgcaaa cgaagaactg catctcctcg ttttacctct aaatagtcag caattttctc
    10141 agaagccaac acagcagtaa tcgtttgatt cgctggaccg atctttttcc cgcctttttc
    10201 ttctagcgtg cgatacaacg attcagtaat ctctgctttg ctgtagccat cgatcagctt
    10261 ttgtggaaca ctggcaactt caaaacaaat agggacatca tctgcgaaac gaatacgttc
    10321 cattcgcaag atcgtgtcat ctttactcaa gtttagtttt tccatttcac ttgagcttgg
    10381 cgaagtcaag aaataagaga ttgtccgact agacggtact cgattttgtg attccataat
    10441 ttctgtaaaa cttgttgcac cagacatttt ttcttgtact ttttctcgcg caacatacgt
    10501 acccgaaccg atctttcttt ctaagattcc ttcatctgcc aagttttgaa ttgcttgtct
    10561 caacgtcata cggctgacat tgaatttcac tgctaattct ctttctgaag gaagacggtc
    10621 tccgactttc catataccct gttcgatttc attttttaat tggtcatgaa tctgtatata
    10681 tacaggtaat tgattcgaca taacgtacac ccgtcctttc gtttcttcta ttataactgg
    10741 tatatacctg attcacaact gaaaaagata aaggtttcta tccatcaaca gaggatttag
    10801 acacacatcg ttctccctac tcctattgtc agaaacaatt ttttagatat gttcaaccta
    10861 gaaaataaca aaagacccta aaatcgatga aaatgccgac ttatggatct tttgttatta
    10921 ttagcggttt ttagccatcc atttttttgt atgattcgtt gggcgttttg attttacctg
    10981 gaccgctttt ccgccttttt tcggaatatt cacttcaata ttgtattttt ctgatttgac
    11041 caaatcatat aacttttcag ctgcttcgtc tactttttct ttccatttca tgtggatccc
    11101 tccaattctt gtagttaact taactctatc ttaattcagc agaatcttca aggaacaagg
    11161 tagcaaagca aaaaaaagag ctctcgaact aac
//
LOCUS       DAZKOM010000096        11052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000096 DAZKOM010000000
VERSION     DAZKOM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.096"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8NF_002311"
     tRNA            12..84
                     /locus_tag="ACG8NF_002311"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8NF_002312"
     tRNA            88..160
                     /locus_tag="ACG8NF_002312"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8NF_002313"
     tRNA            168..249
                     /locus_tag="ACG8NF_002313"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8NF_002314"
     tRNA            267..339
                     /locus_tag="ACG8NF_002314"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8NF_002315"
     tRNA            351..422
                     /locus_tag="ACG8NF_002315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8NF_002316"
     tRNA            439..524
                     /locus_tag="ACG8NF_002316"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8NF_002317"
     tRNA            535..608
                     /locus_tag="ACG8NF_002317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8NF_002318"
     tRNA            618..691
                     /locus_tag="ACG8NF_002318"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8NF_002319"
     tRNA            711..783
                     /locus_tag="ACG8NF_002319"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8NF_002320"
     tRNA            811..884
                     /locus_tag="ACG8NF_002320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8NF_002321"
     tRNA            898..971
                     /locus_tag="ACG8NF_002321"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8NF_002322"
     tRNA            995..1084
                     /locus_tag="ACG8NF_002322"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8NF_002323"
     tRNA            1097..1170
                     /locus_tag="ACG8NF_002323"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8NF_002324"
     tRNA            1189..1264
                     /locus_tag="ACG8NF_002324"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8NF_002325"
     tRNA            1266..1336
                     /locus_tag="ACG8NF_002325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8NF_002326"
     tRNA            1350..1423
                     /locus_tag="ACG8NF_002326"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8NF_002327"
     tRNA            1448..1535
                     /locus_tag="ACG8NF_002327"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8NF_002328"
     tRNA            1557..1628
                     /locus_tag="ACG8NF_002328"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8NF_002329"
     tRNA            1651..1740
                     /locus_tag="ACG8NF_002329"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8NF_002330"
     tRNA            1753..1826
                     /locus_tag="ACG8NF_002330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8NF_002331"
     tRNA            1845..1917
                     /locus_tag="ACG8NF_002331"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8NF_002332"
     tRNA            1921..1993
                     /locus_tag="ACG8NF_002332"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8NF_002333"
     tRNA            1998..2068
                     /locus_tag="ACG8NF_002333"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8NF_002334"
     tRNA            2081..2154
                     /locus_tag="ACG8NF_002334"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8NF_002335"
     tRNA            2166..2237
                     /locus_tag="ACG8NF_002335"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8NF_002336"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8NF_002336"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0472771.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8NF_002337"
     CDS             3826..5298
                     /locus_tag="ACG8NF_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0472772.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8NF_002338"
     CDS             5381..6169
                     /locus_tag="ACG8NF_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0472773.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8NF_002339"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8NF_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0472774.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8NF_002340"
     CDS             7019..8731
                     /locus_tag="ACG8NF_002340"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472775.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG8NF_002341"
     CDS             8734..10506
                     /locus_tag="ACG8NF_002341"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472776.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11052)
                     /locus_tag="ACG8NF_002342"
     CDS             complement(10588..>11052)
                     /locus_tag="ACG8NF_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0472777.1"
                     /translation="LTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKE
                     IIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRH
                     SHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3175 a         1985 c         2523 g         3369 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aa
//
LOCUS       DAZKOM010000097        10824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000097 DAZKOM010000000
VERSION     DAZKOM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.097"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="ACG8NF_002343"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="ACG8NF_002343"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0472778.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="ACG8NF_002344"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="ACG8NF_002344"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0472779.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /gene="dapA"
                     /locus_tag="ACG8NF_002345"
     CDS             2139..3020
                     /gene="dapA"
                     /locus_tag="ACG8NF_002345"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0472780.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /gene="dapB"
                     /locus_tag="ACG8NF_002346"
     CDS             3017..3733
                     /gene="dapB"
                     /locus_tag="ACG8NF_002346"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0472781.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="ACG8NF_002347"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="ACG8NF_002347"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0472782.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="ACG8NF_002348"
     CDS             4450..5451
                     /locus_tag="ACG8NF_002348"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0472783.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="ACG8NF_002349"
     CDS             5448..6809
                     /locus_tag="ACG8NF_002349"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0472784.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="ACG8NF_002350"
     CDS             6769..7920
                     /locus_tag="ACG8NF_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0472785.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="ACG8NF_002351"
     CDS             7895..9064
                     /locus_tag="ACG8NF_002351"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0472786.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="ACG8NF_002352"
     CDS             9239..10618
                     /locus_tag="ACG8NF_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0472787.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3594 a         1900 c         2426 g         2904 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taac
//
LOCUS       DAZKOM010000098        10710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000098 DAZKOM010000000
VERSION     DAZKOM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.099"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..957)
                     /locus_tag="ACG8NF_002353"
                     /pseudo
     CDS             complement(1..957)
                     /locus_tag="ACG8NF_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1448..2386
                     /locus_tag="ACG8NF_002354"
     CDS             1448..2386
                     /locus_tag="ACG8NF_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0472788.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2516..3163
                     /locus_tag="ACG8NF_002355"
     CDS             2516..3163
                     /locus_tag="ACG8NF_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0472789.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3856..4794)
                     /locus_tag="ACG8NF_002356"
                     /pseudo
     CDS             complement(3856..4794)
                     /locus_tag="ACG8NF_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4794..5411)
                     /locus_tag="ACG8NF_002357"
     CDS             complement(4794..5411)
                     /locus_tag="ACG8NF_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0472790.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5425..5595)
                     /locus_tag="ACG8NF_002358"
     CDS             complement(5425..5595)
                     /locus_tag="ACG8NF_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472791.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5943..7433)
                     /locus_tag="ACG8NF_002359"
     CDS             complement(5943..7433)
                     /locus_tag="ACG8NF_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0472792.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7476..7775)
                     /locus_tag="ACG8NF_002360"
     CDS             complement(7476..7775)
                     /locus_tag="ACG8NF_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472793.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8141..8425)
                     /locus_tag="ACG8NF_002361"
     CDS             complement(8141..8425)
                     /locus_tag="ACG8NF_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0472794.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8944..9429)
                     /gene="vanZ-A"
                     /locus_tag="ACG8NF_002362"
     CDS             complement(8944..9429)
                     /gene="vanZ-A"
                     /locus_tag="ACG8NF_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0472795.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9582..10493)
                     /locus_tag="ACG8NF_002363"
     CDS             complement(9582..10493)
                     /locus_tag="ACG8NF_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0472796.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3308 a         1864 c         1657 g         3881 t
ORIGIN      
        1 acttttttct gcttcttttt ctttctgttc ctggtaatca gaaaaattta gtttttctat
       61 atcgttaggt acagcttcca gtaaatgaac aatgaatttt ttgatattcg taataaagtt
      121 ctcttgattg ccttctcttt taccgatttt ttttaaatac gtttcccatt tagccgtcat
      181 ttcagcactc gttaaaaggt gctgactttc aactgcctgg cacaataatt ttcctttttc
      241 agttacaaca agcttattct taatcacttg gatatattct ttttgtttta acgcttcaat
      301 aatacttgct cttgtcgctt ctgttccaat cccctcaact tcttgtaaaa tcttgattgc
      361 ttcttcatca tcaaccgttt tattcgccgt tttcatagca gttaataatg taccctctgt
      421 aaaagctttt ggcggttgcg tttctttttc aacactctta acaccaactt cggcacgttc
      481 gccaatgata acgagtggta acgtttgaac gtcctctttt tcttctttgg tttgttgttt
      541 gaaaagaatt ttccaccctt cttgctttgg tgtctttccg atcgattgaa acagcaaatc
      601 agctactttc gtttgtatct tggtttcttc atacaagtaa tcaggtaaaa acatggcaac
      661 ggtcgtttta acgactaaag cataaatttt tcgttgtaaa tcgtccattt tcgctaatgc
      721 agattcggta ggtacttgtt ttgttgggat aatggcgtgg tgttcctgta ccttactacc
      781 gtccacataa cgctttctag gctctgtttg aaccatttca agatcaagtc ctaaaaaacc
      841 gctatatttg ccaaaattcg cttttaaata agcaaattcg ttctctgtaa taaatggtgt
      901 atctgttctt ggataactca ataattttgc ttcatacagt ccttgcatag cttttaaacg
      961 taaaattttg cgttctaaga cgttttaaat atatctgcgt atatttttat gtgttttatc
     1021 ttttcgtcgg caaatgcgtt aaaatttgcg aaataaaata gctcaagggc gaacgcagtg
     1081 agtgcattta cccttgatct atttttttga gtaaattagc ttttgaaacc gacggaaagg
     1141 aaacacaaaa cttttcagag gcaaggggtt tttaaggggc gcttttagcc ccttgaattt
     1201 atttgcttta agatatattt agttttaaaa tagttttaac gaaaaaaaga cataaaaaaa
     1261 gacgagcaac gctaccaacg ttctcgcccg gaataacaaa ccacacttgt tattcattcg
     1321 atgattagta tatcaatttt tgattttgaa tacaaaacaa aatggacgaa tggatacagg
     1381 cgaaatttat tttgcgattc cactcgtcct tttttcgttc caaaaattat tttaggagcg
     1441 ataaaaaatg aagaaaattg atgaaaaatt agctgcagaa atctcactaa aaaaaccggc
     1501 cgtctctaat agccggttaa gtaatagccg gttaagtggt caaactttgg gaaaatctca
     1561 acccgcatta agttttgatg ccatgacaat cgttggaaat ttgaacaaaa ctaatgctaa
     1621 aaagctatct gactttatga gtgtagagcc acaaatacga ctttgggata tacttcaaac
     1681 aaagtttaaa gctaaggcac ttcaagaaaa agtttatatc gaatatgaca aagtaaaagc
     1741 agatacttgg gatagacgta atatgcgtgt tgaatttaat cccaataaac tcacacatga
     1801 agaaatgatt tggttaaaac aaaatattat cgactacatg gaagatgacg gttttacaag
     1861 attagactta gcttttgatt ttgaagatga tttgagcgat tactatgcaa tgactgataa
     1921 agcagttaag aaaactgttt tttatggtcg taatggcaag ccagaaacaa aatattttgg
     1981 tgtccgtgat agtgatagat ttattagaat ttataataaa aaacaagaac gtaaagataa
     2041 cgcagatgtt gaagttgtgt ctgaacattt atggcgtgta gaagttgaat taaaaagaga
     2101 tatggttgat tactggaatg attgttttaa tgatttacac atcttgaaac ctgcgtgggc
     2161 tactttagaa aaaattaatg agcaagctat ggtttatact ttgttgcatg aagaaagtat
     2221 gtggggaaag ctaagtaaga atactaagac taaatttaaa aaattgatta gagaaatatc
     2281 tccaattgat ttaacggaat taatgaaatc gactttaaaa gcgaacgaaa aacaattgca
     2341 aaagcagatt gatttttggc aacgtgaatt taggttttgg aagtaaaata agttttattt
     2401 gataaaaatt gctaattcag tataattaat atttacgagg tgacataacg tatgaaaaaa
     2461 tcagaggatt attcctccta aatataaaaa tttaaaattt aggaggaagt tatatatgac
     2521 ttttaatatt attgaattag aaaattggga tagaaaagaa tattttgaac actattttaa
     2581 tcagcaaact acttatagca ttactaaaga aattgatatt actttgttta aagatatgat
     2641 aaaaaagaaa ggatatgaaa tttatccctc tttaatttat gcaattatgg aagttgtaaa
     2701 taaaaataaa gtgtttagaa caggaattaa tagtgagaat aaattaggtt attgggataa
     2761 gttaaatcct ttgtatacag tttttaataa gcaaactgaa aaatttacta acatttggac
     2821 tgaatctgat aaaaacttca tttcttttta taataattat aaaaatgact tgcttgaata
     2881 taaagataaa gaagaaatgt ttcctaaaaa accgatacct gaaaacacca taccgatttc
     2941 aatgattcct tggattgatt ttagttcatt taatttaaat attggtaaca atagcagctt
     3001 tttattgcct attattacga taggtaaatt ttatagtgag aataataaaa tttatatacc
     3061 agttgctctg caacttcatc attctgtatg tgatggttac catgcttcac tatttatgaa
     3121 tgaatttcaa gatataattc atagggtaga tgattggatt tagtttttag attttgaaag
     3181 tgaatttaat tttatacacg taagtgatca taaaatttat gaacgtataa caaccacatt
     3241 ttttggttgc ttgtggtttt gattttgaat ttggttttga acttatggac tgatttattc
     3301 agtccatttt ttgtgcttgc acaaaaacta gcctcgcaga gcacacgcat taatgactta
     3361 tgaaacgtag taaataagtc tagtgtgtta tactttactt ggaagatgca ccgaataaaa
     3421 aatattgaag aacaactagc aaaagatttt aaagagttat tttattttaa gtctttataa
     3481 catgagtgaa gcgaattttt aaatttcgat agaaattttt acatcaaaaa gccccctgtc
     3541 aaaattgacg aagggggttt tttggcgcac gcttttcgtt agaaatatac aagattgaaa
     3601 atcgtgtata agtgcgccct ttgttttgaa cttagcacgt tacatcaatt ttttaaaatg
     3661 atgtataagt gcgccctttt aaattttgag tgattatatt ttttgagtta gaaaaaggga
     3721 ttgggaaaat ttcccaaaat aatttaaaaa ataagcaaaa attttcgata gagaatgtgc
     3781 tattttttgt caaaggtgta taccttgact gtgcttgctg ttacattaag tttattttta
     3841 agttattaaa aaagaaatag cttttaaagt ttggctcgct gtcgctttat aaagctgatt
     3901 gacttttgat tgcaaactac ttaaagaaaa caaactcgga ctattcgttt tcttctcttt
     3961 ggtttgaaca tcagcaatta tcccctcttg attgcctatt ttagcttgtt tagaagaaac
     4021 aaaagctaaa agctcctctt gggttttaaa acgctgtgtg gggcttagaa cgcccttaaa
     4081 cgacccttgg tttactttta tactagcttc cacctcgaaa aaaggttctt ttttaaaatt
     4141 ctctatggct tcctggcgct gaaaaataag gtataaggtg ggcgtttgaa cacgtcctag
     4201 tgaaaatgta ccttgtacgc ccttctgttg taaatttaac gtatacaaag ggcttgcgtt
     4261 catgccgatc aaccaatcgg caatttggcg tgtttgcgct tcttgataaa agggatagta
     4321 attcattcca ggttgcaaat tttgaaaacc gcttcggatt acatcttttt ctaagctatt
     4381 gatccatagt cttttaaatg ttttatcttt tgaaaaggca tttgctttat ggataatcga
     4441 ccaggcgata ttttcacctt ctctgtcgct atctgttgca acaataattg tatttgcctt
     4501 tttgagaagt tctgcaacaa ttttaaactg ctttccctta tcttttgcaa cttcaaaatc
     4561 gtatcgatca ggaaaaatcg gcaaagattc aagtttccaa ttttgccact tttcgtcata
     4621 atgacctggt tctgctaatt ccactaaatg cccaaaacca aaggtgataa acgtttcatc
     4681 tgtaaatagt gggtctttga tctcaaaata accgtctttt ttggtgcttt gttttaaagc
     4741 acttgcgtag gctaatgcct ggcttggttt ttcagctaaa ataaccgtac tcattaacta
     4801 tccctctttt cattgttttt tctttgatcg actgtcacgt tatatcttgc tcgatacctt
     4861 ctaaacgttc ggcgattgat tccagtttgt tcttcaactt ctttatcgga taaaccattc
     4921 aaaaacaaat cgaaagcatg ctttaaacgt ggatcatttt ctttaaattt atgctgacga
     4981 cctttgaatt tgcctttttt cttagcaatt tcgattcctt gtgcctgacg ttccttaatt
     5041 ttttttcgtt ctgattctgc ttgatacttg tacaattcaa tgacaaggct attaatcaaa
     5101 cgccttaaat tttcatcttc aataccattc attgagggta aatttaagac ttccagggtt
     5161 gcccccttaa tttgaatttg attcatcaat tctgttaatt ctttattatt tcgtcctaat
     5221 cgatctaatt cagtaacaat aacaatatcc ccttcacgaa tatagttaag catagcttgt
     5281 aattgtgggc gttcgaccga ttgaccgctt aatttgtctg aaaagacctt agaaacgccc
     5341 tgtaacgctt gtaattgccg atctaagttc tgttctttgc tactgacacg tgcataacca
     5401 attttagcca ttttcaacca acctctaaaa ttctctcggt tgcaataacc aatcagcaat
     5461 atctactttt tcaatttcaa attgcttatc agaaattgtc ttttcgtaag cgataaaatc
     5521 ttgcgcatat tgttgctcat taaaaatagc caccacttcg tcattttcta aaactcgata
     5581 aataaatttt ttcattttac tcctcctatt atgcccaact taaatgacct attcaccaag
     5641 tcaattatac tgctaaaatc atattaggac aaataggtat actctaatga cctataaaag
     5701 atagaaaatt aaaaaatcaa gtgttcgctt cgctctcact gcccctcgac gttttagtag
     5761 cctttccctc acttcgttca gtccaagcca actaaaagtt ttcgggctac tctctccttc
     5821 tccccctaat aattaattaa aatcttactc tgtatatttc tgctaatcat tcgctaaaca
     5881 gcaaagaaaa aacaaacacg tatcatagat ataaatgtaa tggcatagtg cgggttttat
     5941 tttcagcctg tatcatagct aaacaaatcg agttgtgtgt ccgttttagg gcgttctgct
     6001 agcttgttta aagtctcttg aatgaatgta tgctctaagt caaaagaatt tgtcagcgcc
     6061 tttatatagc tttctttttc ttcttttttt actttaatga tcgatagcaa caatgattta
     6121 acactagcaa gttgaatgcc accatttctt cctggtttaa tcttaaagaa aatttcctga
     6181 ttcgccttca gtaccttcag caatttatct aatgtccgtt caggaatgcc tagcacttct
     6241 ctaatctctt ttttggtcgt cactaaataa ggcttgtata catcgctttt ttcgctaata
     6301 taagccatta aatcttcttt ccattctgac aaatgaacac gttgacgttc gcttcttttt
     6361 ttcttgaatt taaaccaccc ttgacggaca aataaatctt tactggttaa atcacttgat
     6421 acccaagctt tgcaaagaat ggtaatgtat tccctattag ccccttgata gttttctgaa
     6481 taggcacttc taacaatttt gattacttct ttttcttcta agggttgatc taatcgatta
     6541 ttaaactcaa acatattata ttcgcacgtt tcgattgaat agcctgaact aaagtaggct
     6601 aaagagaggg taaacatgac gttattacgc cctattaaac ccttttctcc tgaaaatttc
     6661 gtttcgtgca ataagagatt aaaccagggt tcatctactt gttttttgcc ttctgtaccg
     6721 cttaaaaccg ttagacttga acgagtaaag cccttattat ctgtttgttt gaaagaccaa
     6781 tcttgccatt ctttgaaaga ataacggtaa ttaggatcaa aaaattctac attgtccgtt
     6841 cttggtatgc gagcaatacc aaaatgatta cacgttagat caactggcaa agactttcca
     6901 aaatattctc ggatattttg cgaaattatt ttggctgctt tgacagattt aaattctgat
     6961 tttgaagtca catagactgg cgtttctaaa acaaaatatg cttgataacc tttatcagat
     7021 ttgataatca tagtaggcat aaaacctaaa tcaatagcgg ttgttaaaat atcgcttgct
     7081 gaaatagttt cttttgccgt gtgaatatca aaatcaataa agaaggtatt gatttgtctt
     7141 aaattgtttt cagaatgtcc tttcgtgtat gaacggtttt cgtctgcata cgttccataa
     7201 cgataaacgt tgggtgtcca atgtgtaaat gtatcttgat tttcttgaat cgcttcctcg
     7261 gaagtcagaa caacaccacg accgccaatc atgcttgatt ttgagcgata cgcaaaaata
     7321 gcccctttgc ttttacctgg cttggtagtg attgagcgaa ttttactatt tttaaatttg
     7381 tactttaaca agccgtcatg aagcacagtt tctacaacaa aagggatatt cattcagctg
     7441 ttctcctttc ctataaatcc tataaaatag gttgtttaat taacttggtt tgctttttca
     7501 ttcaactgtt tcaatattgc atgttttgaa aaagattttt ttcctttata agtcaatttt
     7561 tttccactaa tcgaataaat tattttgtta ttttctatta acttatatat ataatcttcc
     7621 ccctccgaag aaaaatactt atctgatttt gtttctaagt agatatttct cttttctaac
     7681 tctttcttaa acgtttctag tgtatagata tttgctaatt ttcttatctc caataaacta
     7741 ttttttatat aagttttaca ttcatcatga ttcatacaaa ctccaccttc tataaatgaa
     7801 tacaaaaaaa gcaatcaaac gatttccgat tgattgctta acaattctta aattcagtag
     7861 cttagatact tgaaaactct ctgatttccc tatataatga tagtacggtt atataccgtc
     7921 ttcaaacaaa gttaattaaa taacttctta cgagggaaga gttcatctga ctaactgata
     7981 agcgttggtt tggcaatctt atcgggctat gcatttataa aatgtcgtca aacattttat
     8041 aaatgtgtca tggctctttt ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat
     8101 accgctttta aaaaaaataa gcaacgattt cgtgcattat tcacacgaag tcattgcttt
     8161 tttcttcttc catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt
     8221 ctgttggctc atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt
     8281 gttctagtgt ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc
     8341 tactcatttc taattcttgg gagaattttt cttttgattt ggtacctctc atctttttta
     8401 agctttctct aaatgctagt tccaagtttc tcccccctct ttttgtgtaa taaaattcac
     8461 gctttattta aattatagct tttaatatct taataaacaa gtgattaatc acttgtgatt
     8521 tgtgattatt ccaaaagtag gtaatttcac caaccttttt caaaagtttt tatcgacgat
     8581 tattggactg gatcgtgttt cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg
     8641 taattctctt taaaacgtcc tacaaagatt caaaaaaagc atggctgcta gtgggggtac
     8701 cgtcaggaaa atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag
     8761 cactaaaatt gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt
     8821 atcaccggtt ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca
     8881 tctaatccaa ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac
     8941 atcttacacg taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag
     9001 tatacctaca aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa
     9061 accatataat ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc
     9121 tgtcgctcca atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac
     9181 aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa
     9241 tggaataaag attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg
     9301 agtcaagtta agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt
     9361 gaataacact aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat
     9421 ttttcccaat ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg
     9481 tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa
     9541 taatgctgtt cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta
     9601 tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca
     9661 taacgaagat tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc
     9721 tcatattttt ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata
     9781 tattcctcga gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag
     9841 cgaatatgcc atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt
     9901 aaaatgaacc cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc
     9961 attttcgtca agcttgatcc tacatctagt gataaacctg aattatgctc actataacct
    10021 gctggtaagg catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct
    10081 cgatagccac tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc
    10141 tctgaaaatt tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat
    10201 ccatttatta attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg
    10261 cgaacaggat atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta
    10321 attgttttct cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt
    10381 tgatcataat tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca
    10441 taacctaagt atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt
    10501 ctcctcctta actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg
    10561 cacaatctcg tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc
    10621 tactggattt actattatca ccaatgtaga tgaattttca taagtgatac ccatgctgaa
    10681 tttaacgagg ctgaactgcc tgttgacaat
//
LOCUS       DAZKOM010000099        10517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000099 DAZKOM010000000
VERSION     DAZKOM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.100"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8NF_002364"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8NF_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8NF_002365"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8NF_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8NF_002366"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8NF_002366"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0472797.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8NF_002367"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8NF_002367"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0472798.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8NF_002368"
     CDS             5704..6489
                     /locus_tag="ACG8NF_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0472799.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8NF_002369"
     CDS             6502..7353
                     /locus_tag="ACG8NF_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0472800.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8NF_002370"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8NF_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472801.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG8NF_002371"
     CDS             complement(8394..8762)
                     /locus_tag="ACG8NF_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0472802.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG8NF_002372"
     CDS             8912..9394
                     /locus_tag="ACG8NF_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0472803.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG8NF_002373"
     CDS             9431..10441
                     /locus_tag="ACG8NF_002373"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0472804.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAZKOM010000100        10293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000100 DAZKOM010000000
VERSION     DAZKOM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.101"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..1595)
                     /locus_tag="ACG8NF_002374"
     CDS             complement(87..1595)
                     /locus_tag="ACG8NF_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0472805.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1754..1945
                     /locus_tag="ACG8NF_002375"
     CDS             1754..1945
                     /locus_tag="ACG8NF_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472806.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2148..3065
                     /locus_tag="ACG8NF_002376"
     CDS             2148..3065
                     /locus_tag="ACG8NF_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0472807.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3058..4131
                     /locus_tag="ACG8NF_002377"
     CDS             3058..4131
                     /locus_tag="ACG8NF_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472808.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4279..4797
                     /locus_tag="ACG8NF_002378"
     CDS             4279..4797
                     /locus_tag="ACG8NF_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472809.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4778..5170
                     /locus_tag="ACG8NF_002379"
     CDS             4778..5170
                     /locus_tag="ACG8NF_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0472810.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5246..6181
                     /locus_tag="ACG8NF_002380"
     CDS             5246..6181
                     /locus_tag="ACG8NF_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0472811.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6314..7093
                     /locus_tag="ACG8NF_002381"
     CDS             6314..7093
                     /locus_tag="ACG8NF_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0472812.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7086..7403
                     /locus_tag="ACG8NF_002382"
     CDS             7086..7403
                     /locus_tag="ACG8NF_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0472813.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7647..9257
                     /locus_tag="ACG8NF_002383"
     CDS             7647..9257
                     /locus_tag="ACG8NF_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0472814.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9414..10262
                     /locus_tag="ACG8NF_002384"
     CDS             9414..10262
                     /locus_tag="ACG8NF_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0472815.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3241 a         1803 c         2075 g         3174 t
ORIGIN      
        1 tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc gattaagcta atagtttcgt
       61 tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga agggtagaca
      121 ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt tttattgacc
      181 atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata agcaacttga
      241 gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt gatgcttagt
      301 gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc aagaactaat
      361 ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc atttaagata
      421 actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac ttcatctggt
      481 aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg aatcttatct
      541 gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc gacttcttcc
      601 ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt gaagtcgcat
      661 tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac aacggtatca
      721 tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg ataaacagat
      781 tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc aacatcccga
      841 ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat catattgaca
      901 tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt caacggaaca
      961 ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt ttggctttca
     1021 tgatacagtt ggttcaagca agtagcgagc tttccacttc ctgggccagg agctgtcact
     1081 acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc ttctgagacg
     1141 attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac cttgatgttt
     1201 cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata gcgagtgata
     1261 acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg caagatatcc
     1321 atatcataag taatcccata atcaccacgg atcttattac gttcaatatc tccagcatag
     1381 acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat tttcgcatct
     1441 tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactaactt cccaccaaat
     1501 tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga ctgctcttca
     1561 atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta agtactaaag
     1621 cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa
     1681 agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata gtttttgata
     1741 aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac actatatccg
     1801 ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac aattgacgtt
     1861 tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca ttcatttcac
     1921 aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt cgattctgtc
     1981 ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct
     2041 gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa tggtactatt
     2101 gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg aaagagccag
     2161 atacaaatgc aaattatgag ccagacgaac tagaggagac gaacgcggat caggatacag
     2221 gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat ctttcccaat
     2281 tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc cctgagatct
     2341 accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg
     2401 agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa tttcctttta
     2461 tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc acttattacc
     2521 atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt ttgcctaaga
     2581 tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac aaaaaaatca
     2641 atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta gatgaacaaa
     2701 taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc ttgaatcgta
     2761 aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta tctaatgaaa
     2821 aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa aaagaagcat
     2881 tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc
     2941 aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat gaattcttga
     3001 tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt tccgagtatg
     3061 aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga tcatgaagaa
     3121 acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga agaaatccct
     3181 atgctagagg aaacaaaaat acatgaaaaa gaaaaagtaa ctccgacgaa tacgaatact
     3241 gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga aggaaaagag
     3301 caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt agcggattta
     3361 cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc atatgatcgt
     3421 tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat cgaatacttc
     3481 acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa ttcagcttat
     3541 gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca aaacgaatta
     3601 gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc gatgagtctc
     3661 ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt gctttctttg
     3721 atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat attcaatcac
     3781 aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa tcagacatta
     3841 ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa
     3901 gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat ggaaaagatc
     3961 aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat aacaacaaac
     4021 gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc tgtcattgat
     4081 ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg attggataag
     4141 ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc agttatccag
     4201 aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact tgctgatgaa
     4261 ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt attgtctgct
     4321 gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc aatgactgct
     4381 atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt catgagtctt
     4441 tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg ccaatggatc
     4501 agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac agtgcaaagg
     4561 aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg aataggtatg
     4621 accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta ccttcaaggg
     4681 gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt ttttctgcca
     4741 gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa tttatagttt
     4801 gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct atacacaatc
     4861 ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga
     4921 aaatggtcaa aacttttcgg aaaaagaagt ggtcttcaaa aaaggcgatg atttactgag
     4981 tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt cgcttgaagg
     5041 gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg ggaaaagtgc
     5101 tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc
     5161 taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat tcagtgagtt
     5221 cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa tgttttccgt
     5281 aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg gctatgcaat
     5341 cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt gcagcaatca
     5401 gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt tgcatcaatt
     5461 tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc ttttcttcgg
     5521 cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat cccatgaagt
     5581 gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg aaggaagttc
     5641 gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt ccacgatgcg
     5701 ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag acttgtgtgc
     5761 tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa ccgggattgc
     5821 tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag ccgccatttt
     5881 cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc
     5941 aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata taaaaacagt
     6001 tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg gtaacaaaga
     6061 gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga
     6121 caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt ctcgtaaata
     6181 aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt tttaaataaa
     6241 acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg ttgtaattaa
     6301 aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt
     6361 gatgccactt tgtataagct atataccagt aggattagct tgcggagtat tgttgcaaaa
     6421 agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt ccggtggcgc
     6481 gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga cattattgat
     6541 ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg gttttatgaa
     6601 aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg aaaactatgc
     6661 ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg ctttatatgt
     6721 caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt ttttcggctc
     6781 ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt tcattttcat
     6841 gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct caggcgtatt
     6901 aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag caacagtcat
     6961 tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact
     7021 aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac aggaggccaa
     7081 tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc gtggcatata
     7141 tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc tggttcagtg
     7201 actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa gatgttttca
     7261 ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg attctagtag
     7321 caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg
     7381 tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga ttgcgacata
     7441 agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg atgcaagttt
     7501 attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt ttattttagg
     7561 aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga tgtttatttt
     7621 atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga acattttggc
     7681 taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt ccctggctaa
     7741 gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat gttggctatc
     7801 ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg attgcaaaaa
     7861 aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt tttagaagta
     7921 cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat ttatttgctc
     7981 ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta gctatcagaa
     8041 gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga tatttccaag
     8101 gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct gtgcgagtga
     8161 tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc atattagaag
     8221 ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt atccatatgt
     8281 atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt tccagccgtt
     8341 atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca ctaccgttta
     8401 tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg atgaaaccac
     8461 tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta ttcagccgtg
     8521 cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga acagtagcta
     8581 taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa
     8641 cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg actggaatgt
     8701 ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa agtgtcggat
     8761 atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc ctatctacga
     8821 tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa gcaattatcc
     8881 tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg atgagcttat
     8941 caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg tgcaaagaat
     9001 accgaatagc acctttagca aagatcagca attttttctt aaaaactttc cgctccaccc
     9061 tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga ttgtcaatgg
     9121 aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc ggcttagtat
     9181 ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata
     9241 gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa acggcaactc
     9301 gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct aatcttactt
     9361 atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac
     9421 ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac catcccgact
     9481 tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa ggaatcgata
     9541 tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct actacttcac
     9601 aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa gggtatgaca
     9661 cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca ttatttgcga
     9721 tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc actagtatgc
     9781 cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag tcccttgatg
     9841 aaatacttgc acatatcgat cgaatccgag ataataccta tgcttatctg attgtagatg
     9901 atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg attggcagct
     9961 tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa
    10021 tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga agaaataacg
    10081 aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa gtagcgattg
    10141 cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg
    10201 gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat
    10261 aattcctttt attcaaaaaa cataccaaaa ata
//
LOCUS       DAZKOM010000101        10152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000101 DAZKOM010000000
VERSION     DAZKOM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.102"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG8NF_002385"
     CDS             complement(46..366)
                     /locus_tag="ACG8NF_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0472816.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG8NF_002386"
     CDS             complement(514..2748)
                     /locus_tag="ACG8NF_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0472817.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG8NF_002387"
     CDS             3227..3493
                     /locus_tag="ACG8NF_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0472818.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8NF_002388"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8NF_002388"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0472819.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG8NF_002389"
     CDS             5384..6430
                     /locus_tag="ACG8NF_002389"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0472820.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="ACG8NF_002390"
     CDS             6447..7670
                     /locus_tag="ACG8NF_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0472821.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="ACG8NF_002391"
     CDS             7759..8646
                     /locus_tag="ACG8NF_002391"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0472822.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="ACG8NF_002392"
     CDS             8732..8962
                     /locus_tag="ACG8NF_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0472823.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="ACG8NF_002393"
     CDS             complement(9019..9984)
                     /locus_tag="ACG8NF_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0472824.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3237 a         1788 c         2047 g         3080 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aaataatgga caagttttag ggaattctat agttctgttc acagaaaata
    10081 ctttgataat caaaatatgt gagatagatg atttttctaa taaaagaaat tataaatgct
    10141 gatttatcag tt
//
LOCUS       DAZKOM010000102        10052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000102 DAZKOM010000000
VERSION     DAZKOM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.103"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..734
                     /locus_tag="ACG8NF_002394"
     CDS             255..734
                     /locus_tag="ACG8NF_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0472825.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            767..2995
                     /locus_tag="ACG8NF_002395"
     CDS             767..2995
                     /locus_tag="ACG8NF_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0472826.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3124..4767
                     /locus_tag="ACG8NF_002396"
     CDS             3124..4767
                     /locus_tag="ACG8NF_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0472827.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4840..5385)
                     /locus_tag="ACG8NF_002397"
     CDS             complement(4840..5385)
                     /locus_tag="ACG8NF_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0472828.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5408..5893)
                     /locus_tag="ACG8NF_002398"
     CDS             complement(5408..5893)
                     /locus_tag="ACG8NF_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0472829.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5883..6695)
                     /locus_tag="ACG8NF_002399"
     CDS             complement(5883..6695)
                     /locus_tag="ACG8NF_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0472830.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6834..8366)
                     /locus_tag="ACG8NF_002400"
     CDS             complement(6834..8366)
                     /locus_tag="ACG8NF_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0472831.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8475..9506)
                     /locus_tag="ACG8NF_002401"
     CDS             complement(8475..9506)
                     /locus_tag="ACG8NF_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0472832.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3355 a         1852 c         1929 g         2916 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca
       61 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
      121 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
      181 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
      241 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
      301 aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac
      361 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
      421 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
      481 tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
      541 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
      601 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
      661 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
      721 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
      781 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
      841 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
      901 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
      961 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
     1021 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
     1081 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
     1141 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
     1201 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
     1261 tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat
     1321 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
     1381 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
     1441 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
     1501 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
     1561 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
     1621 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
     1681 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
     1741 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
     1801 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
     1861 agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
     1921 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
     1981 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
     2041 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
     2101 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
     2161 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
     2221 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
     2281 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
     2341 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
     2401 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
     2461 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
     2521 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
     2581 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
     2641 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
     2701 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
     2761 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
     2821 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
     2881 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
     2941 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
     3001 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
     3061 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
     3121 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
     3181 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
     3241 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
     3301 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
     3361 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
     3421 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
     3481 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
     3541 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
     3601 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
     3661 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
     3721 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
     3781 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
     3841 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
     3901 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
     3961 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc
     4021 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
     4081 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
     4141 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
     4201 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
     4261 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
     4321 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
     4381 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
     4441 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
     4501 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
     4561 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
     4621 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
     4681 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
     4741 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
     4801 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
     4861 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
     4921 acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat
     4981 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
     5041 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
     5101 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
     5161 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
     5221 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
     5281 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
     5341 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
     5401 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
     5461 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
     5521 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
     5581 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
     5641 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
     5701 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
     5761 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
     5821 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
     5881 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
     5941 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
     6001 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
     6061 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
     6121 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
     6181 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
     6241 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
     6301 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
     6361 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
     6421 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
     6481 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
     6541 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
     6601 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
     6661 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
     6721 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
     6781 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
     6841 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
     6901 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
     6961 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
     7021 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
     7081 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
     7141 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
     7201 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
     7261 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
     7321 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
     7381 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
     7441 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
     7501 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
     7561 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
     7621 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
     7681 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
     7741 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
     7801 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
     7861 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
     7921 tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc
     7981 actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat
     8041 acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa
     8101 gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg
     8161 tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag
     8221 cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag
     8281 ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa
     8341 ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa gtaatataca
     8401 aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag
     8461 atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa
     8521 aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca
     8581 cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt
     8641 tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga
     8701 atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc
     8761 gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt
     8821 ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat
     8881 acatctttac gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg
     8941 gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt
     9001 tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata
     9061 tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct
     9121 tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt
     9181 gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg
     9241 aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa
     9301 tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac
     9361 agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct
     9421 tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc
     9481 agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc attttctata
     9541 ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt
     9601 ttaactaagt actagctaaa agatatgaag taagaagata catttttagt tattttatta
     9661 gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt
     9721 catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc
     9781 gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt
     9841 gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt
     9901 actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta atcctttttt
     9961 atataagttg tttgcttatt gatggattgg ataaattaaa ccatcacaac gagtggaaat
    10021 tatatgaaat ttttcataaa aattcatata ga
//
LOCUS       DAZKOM010000103         9811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000103 DAZKOM010000000
VERSION     DAZKOM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.105"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG8NF_002402"
     CDS             complement(34..2154)
                     /locus_tag="ACG8NF_002402"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0472833.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG8NF_002403"
     CDS             2384..3562
                     /locus_tag="ACG8NF_002403"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0472834.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8NF_002404"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8NF_002404"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0472835.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8NF_002405"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8NF_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0472836.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG8NF_002406"
     CDS             complement(4915..6168)
                     /locus_tag="ACG8NF_002406"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0472837.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8NF_002407"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8NF_002407"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0472838.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG8NF_002408"
     CDS             7746..9092
                     /locus_tag="ACG8NF_002408"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0472839.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG8NF_002409"
     CDS             complement(9400..9786)
                     /locus_tag="ACG8NF_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0472840.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAZKOM010000104         9594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000104 DAZKOM010000000
VERSION     DAZKOM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.106"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG8NF_002410"
     CDS             complement(<1..641)
                     /locus_tag="ACG8NF_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0472841.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG8NF_002411"
     CDS             complement(643..1407)
                     /locus_tag="ACG8NF_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0472842.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG8NF_002412"
     CDS             complement(1415..2092)
                     /locus_tag="ACG8NF_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0472843.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG8NF_002413"
     CDS             complement(2129..3514)
                     /locus_tag="ACG8NF_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0472844.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG8NF_002414"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8NF_002414"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8NF_002415"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8NF_002415"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0472845.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG8NF_002416"
     CDS             4453..5310
                     /locus_tag="ACG8NF_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472846.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG8NF_002417"
     CDS             complement(5391..5726)
                     /locus_tag="ACG8NF_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472847.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG8NF_002418"
     CDS             complement(5793..7163)
                     /locus_tag="ACG8NF_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0472848.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG8NF_002419"
     CDS             complement(7241..7777)
                     /locus_tag="ACG8NF_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0472849.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG8NF_002420"
     CDS             8086..9567
                     /locus_tag="ACG8NF_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472850.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1970 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt attc
//
LOCUS       DAZKOM010000105         9570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000105 DAZKOM010000000
VERSION     DAZKOM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.107"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..818
                     /locus_tag="ACG8NF_002421"
     CDS             <1..818
                     /locus_tag="ACG8NF_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0472851.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="ACG8NF_002422"
     CDS             938..1138
                     /locus_tag="ACG8NF_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0472852.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="ACG8NF_002423"
     CDS             1214..1411
                     /locus_tag="ACG8NF_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472853.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="ACG8NF_002424"
     CDS             complement(1708..3255)
                     /locus_tag="ACG8NF_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0472854.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="ACG8NF_002425"
     CDS             complement(3339..4142)
                     /locus_tag="ACG8NF_002425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0472855.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8NF_002426"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8NF_002426"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0472856.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8NF_002427"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8NF_002427"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0472857.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8NF_002428"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8NF_002428"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472858.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="ACG8NF_002429"
     CDS             complement(6527..7456)
                     /locus_tag="ACG8NF_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0472859.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="ACG8NF_002430"
     CDS             7651..8334
                     /locus_tag="ACG8NF_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0472860.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..9538
                     /locus_tag="ACG8NF_002431"
     CDS             8495..9538
                     /locus_tag="ACG8NF_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0472861.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2931 a         1956 c         1900 g         2783 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
     9541 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DAZKOM010000106         9082 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000106 DAZKOM010000000
VERSION     DAZKOM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.109"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8NF_002432"
     CDS             168..362
                     /locus_tag="ACG8NF_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472862.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8NF_002433"
     CDS             631..930
                     /locus_tag="ACG8NF_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472863.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8NF_002434"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8NF_002434"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472864.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8NF_002435"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8NF_002435"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0472865.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8NF_002436"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8NF_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0472866.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8NF_002437"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8NF_002437"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0472867.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8NF_002438"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8NF_002438"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0472868.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8NF_002439"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8NF_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0472869.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8NF_002440"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8NF_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0472870.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8NF_002441"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8NF_002441"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0472871.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8NF_002442"
     CDS             7521..8291
                     /locus_tag="ACG8NF_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0472872.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..>9082)
                     /locus_tag="ACG8NF_002443"
     CDS             complement(8564..>9082)
                     /locus_tag="ACG8NF_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0472873.1"
                     /translation="NSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHH
                     LQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDY
                     DKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGN
                     DYAVKVIENDLK"
BASE COUNT         2843 a         1865 c         1437 g         2937 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa tt
//
LOCUS       DAZKOM010000107         9018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000107 DAZKOM010000000
VERSION     DAZKOM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.110"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACG8NF_002444"
     CDS             <1..1101
                     /locus_tag="ACG8NF_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0472874.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACG8NF_002445"
     CDS             complement(1280..1615)
                     /locus_tag="ACG8NF_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0472875.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACG8NF_002446"
     CDS             complement(1599..1862)
                     /locus_tag="ACG8NF_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0472876.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACG8NF_002447"
     CDS             complement(2322..3125)
                     /locus_tag="ACG8NF_002447"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0472877.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACG8NF_002448"
     CDS             complement(3103..4227)
                     /locus_tag="ACG8NF_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472878.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACG8NF_002449"
     CDS             complement(4243..5058)
                     /locus_tag="ACG8NF_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472879.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACG8NF_002450"
     CDS             complement(5048..5920)
                     /locus_tag="ACG8NF_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472880.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACG8NF_002451"
     CDS             complement(6005..7294)
                     /locus_tag="ACG8NF_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0472881.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACG8NF_002452"
     CDS             complement(7438..7950)
                     /locus_tag="ACG8NF_002452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472882.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACG8NF_002453"
     CDS             complement(8017..8160)
                     /locus_tag="ACG8NF_002453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0472883.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8643..>9018)
                     /locus_tag="ACG8NF_002454"
     CDS             complement(8643..>9018)
                     /locus_tag="ACG8NF_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0472884.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DAZKOM010000108         8864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000108 DAZKOM010000000
VERSION     DAZKOM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.111"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..267
                     /locus_tag="ACG8NF_002455"
     CDS             82..267
                     /locus_tag="ACG8NF_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0472885.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            280..528
                     /locus_tag="ACG8NF_002456"
     CDS             280..528
                     /locus_tag="ACG8NF_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472886.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            559..729
                     /locus_tag="ACG8NF_002457"
     CDS             559..729
                     /locus_tag="ACG8NF_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472887.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(831..1298)
                     /locus_tag="ACG8NF_002458"
     CDS             complement(831..1298)
                     /locus_tag="ACG8NF_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472888.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            1457..1774
                     /locus_tag="ACG8NF_002459"
     CDS             1457..1774
                     /locus_tag="ACG8NF_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472889.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            1707..2186
                     /locus_tag="ACG8NF_002460"
     CDS             1707..2186
                     /locus_tag="ACG8NF_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0472890.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            2203..2379
                     /locus_tag="ACG8NF_002461"
     CDS             2203..2379
                     /locus_tag="ACG8NF_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472891.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            2636..3067
                     /locus_tag="ACG8NF_002462"
     CDS             2636..3067
                     /locus_tag="ACG8NF_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472892.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            3073..3390
                     /locus_tag="ACG8NF_002463"
     CDS             3073..3390
                     /locus_tag="ACG8NF_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0472893.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            3509..4195
                     /locus_tag="ACG8NF_002464"
     CDS             3509..4195
                     /locus_tag="ACG8NF_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0472894.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            4170..5519
                     /locus_tag="ACG8NF_002465"
     CDS             4170..5519
                     /locus_tag="ACG8NF_002465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0472895.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            5510..5983
                     /locus_tag="ACG8NF_002466"
     CDS             5510..5983
                     /locus_tag="ACG8NF_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0472896.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5985..6485
                     /locus_tag="ACG8NF_002467"
     CDS             5985..6485
                     /locus_tag="ACG8NF_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0472897.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            6500..8812
                     /locus_tag="ACG8NF_002468"
     CDS             6500..8812
                     /locus_tag="ACG8NF_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0472898.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         3149 a         1467 c         1951 g         2297 t
ORIGIN      
        1 atagcttaaa agctaatagg tgtcaacgat ttagctaatt ttttagctta caaattattt
       61 gtttagaaag gagctatttt tatgtctgag aatttagact taaaaattcg agcggagatg
      121 agaaaaagaa gaatgacttt caaagaacta gctgcgctta tcggtatttc aggagcttat
      181 ttatcagata ttctaaacgg caatcgtgat ggaaagaaag cacaacagca tatcgaaaca
      241 gtgaaaaaaa tattggacat ccgatagggg gggtaggcga tgacaaaact aaaaaaacaa
      301 gattttgtaa aaaaatataa ttattctcca tctacttatc aacgtcgaat gtcggaacta
      361 aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga agtttggatc
      421 aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt acgcacaaga
      481 aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa aacagaacat
      541 tttgagaggt gaaggttaat gggtaaattc aacagagcat tagtattcag cgcaccgcta
      601 atcgtctatg ctttagggct ttggggaagt agacaagcgt tgataggaac gattgtgtac
      661 atggtttgga tttttatagg gctggatgaa gctgagtaca aaacaaaaaa gccagtcggg
      721 agggactgac taatgagtaa ggacgatatt gtatttctgt taattatgtt ttgtgggatt
      781 ggatcaataa taattaattt gtatttgatt attaaagaaa aatttttcaa ttaaaatgag
      841 tcttttagct ttaacttaaa ttttttttga taattagctg ttcttatgac cattacaagt
      901 ttttcaccag gtaatatatc tttcatatct aaaacttctg aaaaagcgac tgagtagcca
      961 ggagagattg cttgtgccaa cggtatagga gataaaaggc tacttttatg aatgcttccc
     1021 atatcaaaac cattggcgtg ataatgtgaa caattaattt tcttaccgtt gtaaagatac
     1081 gtacattcga taattgagtt tggcattttt gatgggtttg caattacgcc agtaagaatc
     1141 attcgattac ttctatcatg cttaactaaa aaataggaga gcgtaatttt tcttttatct
     1201 ttaaataagt tatagaggga taaaaaaaat cctgaaatag aaatgattaa agtaatagcg
     1261 acttggtaat cgctcaaaat ttttatcaag taattcacca cctttttact caaattatat
     1321 caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa aaaagacacg
     1381 aacgccggca agcatgatcg tgtcagggaa ataaactatc tttctatatt ttaccacaga
     1441 aaggaatgaa ccgtaaatgc taattgcaac ggatacacta gaccgcatct ttttaaaaga
     1501 cgaatacaaa ctgcgcaata tagatgcgtc aggaatttta gtatttgatc tttatgacaa
     1561 tggaaaaatt ggtatctatc aagcaagtga tattgaagaa acaaaccttg ctttcgaaca
     1621 aattgatgat tctgtggaat tggatttaga tgaggcaatc ctagcttttg aacaaattgc
     1681 aaaattatta aaggaggcac aaaagaatgg caactcttta ccaactcagc gagtcatata
     1741 tcaaagtcct ggaactggca gaacaattgg atgaagaaat tcttcgcgat actctcgatt
     1801 cgattaatga agcgatcgag tataaggcag aaaacttagc aaaaatagtt aaagaagtag
     1861 aagggaaagc tgagttaatc gatagtgaaa ttaaacgtct gcaggaacgt aagacatcgc
     1921 ttttgaacaa tgctaagagt atcaagcact atttacaaga ggaaatggaa aagactggta
     1981 aaacgaagat taaaggtgaa ttattcaaca ttggaattca aaataatcca gtatcggtca
     2041 atgtaatcaa tgagaactta attccaaaag gattctttac ccctgtgcct cccaaattgg
     2101 ataaaaagca attgaaggag gaactgaagc acggagatat tcctggtgct gaactcgtcc
     2161 aaacaaaagg tttgaggata agatgatgaa ggaggagaaa taatggaaca cgaaaacgta
     2221 aaagacgctt tgaaagctgc tatcgaaatc gcggaagcta aaggaataaa ggttgacggc
     2281 aaaccagcaa ctgtccatga cattcaaaat ttgacgaaag aacatcttta ttttatcgcc
     2341 gacttgcttg gtttgtcgga attgtattta gacaaataac cccacacgga aggagtggct
     2401 agggtggttg tattacaagt aatagtctgc gtaacattca tttggtcgtg cataccggtt
     2461 tgtatatata tcatgaagaa gcttcgccct gagtcttaca aattatacgc aatttacagc
     2521 ataacagtag ctcttgcatt tacattgttt gcaatttcta ggcttccgtt ttaatcgtgg
     2581 gaatgaattt ttaatgaact aaattatcta taaagcaaaa aaaggagaaa caacattgaa
     2641 aaacaaacta gcaaaaacaa cagcaatcat cggactagca ctaggtagcg gcgttatcgg
     2701 ctacgcagca agcaatgcgt ttcaagattt ggacacgatc aaagcaaatt tcaacacagt
     2761 cctacaatac ggtcaaacga aatcgcaacg tgtgtcagaa cttgaatcac agctatccaa
     2821 caacactcgc acacaggagc agctgaaagc cgaaattgag caaatcaaat cggacaagca
     2881 gaaggaaatt gaagccaagc aacgagaagt cgaacaaaag caacaggaga tcgctacaaa
     2941 gcaacaggaa gccgatagct tgcgccaaca actatccaca gtgcaaaacg acaaggagca
     3001 gttagaacag cgcgtgagcg agttacggca gtatacggat caaaaggtag gggagttggg
     3061 gaaatgaaag agttgactat ttatgaccca aatatttcat gggctaaaca cacaattaaa
     3121 gtttctttta tgatttgggg ttacaaagga tatgttactt acaaagtggg cggtaacact
     3181 aagggtttat cattaatagc tattgattca gatgatctat atgatgctaa tttcgaggat
     3241 aacccagtta atttccgtga ccttgatgaa gattggttct ctatggaact tactaacgat
     3301 aaaggcgaca gtacacttgt agaagatgaa ttcgacagac taggtgatta tattgtcggt
     3361 gttgaaatta ttgcgcacga accagagtag gagagtgaat acaaaatgat taaaagaatc
     3421 actatatcag ttgaagaaga tagtggatca acaatgcagc ataccgacaa tcgaaaaact
     3481 tttttaacca atagaaagga gaattttgat gcagattaag aaggcgacgg atatcgaaca
     3541 aaccaaggga acatatttga tctacggtgc tccaggaaaa ggtaaaactt caacggctaa
     3601 atattttcca gggaaaacac tgatccttga tattgatcgg acttcaaaag tccttagagg
     3661 tgagaaaaat gtcgatatcg tatatatcga caacgaagat acctggaacg actggggcaa
     3721 cacgttggct gatttaacta ccaattatgt aggtgtttat gacaacgtgg ttgtcgataa
     3781 tgtcagcgaa ttggaacggt gtattctgtc cagtttaggc gcagaaggaa aaaacaacgg
     3841 cgttccttcg caaggcgatt atcaatatat gcaatttaga atggtgaaca gcctacgcta
     3901 tatgaaaaat ttagacagca accttatctg gaccgcatgg gaagaaatcg atttgtggac
     3961 ggattcggat ggatcttctt acaacatcgc tttgccgcag atcaatcgaa agattcgaaa
     4021 caatattttg ggattatgtg acgttgttgg tcgattgatg gtaaaggaag acgatgaacg
     4081 cggatttatt ttacaagcca cagattcaac ctatgccaaa aatcaattgg ataatagatc
     4141 tggctgtaag caaagtgagc tgattttaga tggaattgta cgactaccag aatgatttag
     4201 tcgatcgtgc aagacaagcc tatgttgatg gttacaaggc accatgtatc gtatcaccgt
     4261 gcggttctgg aaaatcagtc atggttgcag agatcgctaa acgaaccaca cgaaaaggca
     4321 acagagtgtt gttcttggtc catcgtcgtg aattgattga tcagatcaaa gatacgtttc
     4381 aaaagatggg cgtagataat cagcttgtaa atttcggcat ggttcaaaca gtcgtacgac
     4441 atcttaaaac catcaagaaa ccacaactga tcatcactga cgaaaaccat cacgggttag
     4501 cagcgtccta cagaaaaatc tatgagtatt attcggacgt tccgcgatta ggttttaccg
     4561 cgacacccat ccggttaaac ggtagtgggt tgggggatgt gaacgacttg ctaattgaag
     4621 gagtatcggc taaatggttg attgagaatc atagattagc tccttatgaa tattatgcac
     4681 ctaaattgat cgatacagcc gaattaaaga aagcttcgac tggagatttt acaaagaaat
     4741 caatggataa ggcagtgaag aatactattt atggcgacgt gttgaaacat tataaaacgt
     4801 tagcagaagg tgaacaggca atagcatact gtcacagtat cgatgcaagt aaacacactg
     4861 ctgaaatatt caacgatgct ggttataaag caacacatat cgatgcaaaa actccaaaag
     4921 atgaacgtgc agatattatc gaagcgttca gaaatcatga aattaaaatc ctatgcaatg
     4981 ttgatttgat cggagaaggg tttgatgttc ctgattgttc gactgtaatt atgttgagac
     5041 ctactcaatc gttatcattg tacattcagc aatcgatgag ggggatgcgt taccgaccag
     5101 ataaaacgtc aatcatcatt gaccatgtgg gtaatgtgaa ccgttttgga ttgcctgata
     5161 tggatcgtga atggagttta gatacaaaaa agaaaacaaa aactgatagt gatttatcta
     5221 tcgttcaatg cgcgttttgt tttggtgctt acgaacggcc aaaaggcgat aatatttgtc
     5281 cgtattgcgg agagttgcag ccgattgaag aacgaaagtc agagttagaa atcgatgaat
     5341 cggctgaatt aatgaaagtc ggagaaacga aaattacttt gaattttgag aacaataaat
     5401 attacaacat gacggaagat gaagcagaaa gtatagagga cttatatgcc atagcaagag
     5461 ctaaaggatt taaacctggt tgggcttata tggctgctaa gaggaagggg tggttgtagg
     5521 aatgggcggt atttttggta aaaaagcaga agatgttcaa ggttcaataa taaatggcgt
     5581 aacaataata ggtgatactg gaaaaagaga tagttcaggc aatcaaattg taatcgctag
     5641 aaataaaaat aacgaagttt ttgaatcttc tctatctaat ataaaaagag gtgttgttaa
     5701 cgggacaggc gaagagagtg gctacgttaa tttaaaaaaa gctaatgaaa aataccatgt
     5761 tgaaggaact tacgttaagg ctttgtctgt cggtggaaca aaaggcgtta attgggataa
     5821 acgaagaaat aaatggaaag cacgattata tttcaaagga gaatatgtct tacaaaagga
     5881 tttcaacacc aaagaagaag caatccaagc acgacttgca gcagaagaaa aatatttcaa
     5941 acctatctta gaaaaatatg aaaataaaaa ggagaatgat taacatgaca ggatttaact
     6001 tagacttttc aaaagcgcaa caagggaacg aaatcaaaga cggtacgtac gaagtggtcg
     6061 taaacaaagc tgtagaaaat gcaactaaat caggcgcaga atttatcgat atcgatttga
     6121 tcgttcgtaa cgatgtggat caaccgttcc aaaataaaca cattttcgca aaaatctgga
     6181 aagccaaagc taccggcaaa tataacgaag gtatgatcat ggcaattgca caagcgctac
     6241 agttggaaga cggaaaatca tataacgggt ttgatgaatt gctggcggac tttgttttga
     6301 aaaccgcctc agtacgcgtg aaaactgaag agtcaaatgg atataaaaac gtcaatgtga
     6361 agtcttggga taaaacgaac acacggggcg taatgaatca ccaattcaaa aatggtgatg
     6421 aaccatcctt cggaccggaa cgttcaagag cgacaacggt tagaaatgac aatcttccat
     6481 tttaattgag gtgtaatcaa tgtatgaaaa aattccaaaa gagcttaaaa atctcaaaca
     6541 atggtgtgtt tataagctag tttgggatga aaagcggcag aagcacacga agatccctta
     6601 taacgccaac aacgggcata aagccaaatc caatgacgag agtacctggt ctgattttca
     6661 gaccgctctc gctgctattg atcactacgg aatgagtgga ctggggttct ttttcaaacc
     6721 accttatttc ggcattgata tcgataatgc tgaaggtgaa gttgagcgat ttaaatcagg
     6781 tgatatagaa gaaaatatta tctatgaatt tatcgaaagc atgagatcct atgctgagta
     6841 ttctcaatca ggtactggca tccacattat tgctcgcgga gaattgcccg gcggaagacg
     6901 caggaaaggt gacgtagaaa tgtaccagaa cggccgattc ttcgttatga ctggaaatgc
     6961 cgcatcaaaa tatttagaaa taactgagcc gaatccaaag gatatcaaac gcctatatga
     7021 tcgctacgtt ggagatgaaa aaatcatcca attcaaagaa gaaaatccgc tgatgaatac
     7081 cgttgattta ccggttgaag aaattatcaa acgtgctgaa tcgtcttccc aaggggcaag
     7141 attcaaagtc tttatgaacg gcggttggga agctttttat tcctcgcaat ccgaagcgga
     7201 tatggcgttc gccaacgact tggctttttg gaccggtaga gactttgaaa aaatggatga
     7261 gattttcaga gcgtctgcaa tgatccgtag caaatatgac caaaaaaggg gatcggcaac
     7321 ttacggagaa tctctactaa ataaggcaat tgctgataca aattccgtct acaaccccaa
     7381 acggaaatct gattttaaga tttttatcaa agaccaggaa cagccaaaag aggaaaaata
     7441 ctattcgtac gacgacaccg gcaacgcgga tcggtttact gatatctacg gaacgttagt
     7501 caaatattcg tacattgata aatcttggta ttactacgat gggaaagttt ggctacagga
     7561 caataccggc gaagttcgga aaatgatcga tacgacagta gacatcatgg gaaaagaacc
     7621 gttaacgatc cctgaaggcg cagacgatga aaccaaagaa gctttgatga aagcgaaaga
     7681 aaaacacgtc aagcgttctc gcagcaatgc cggaaaaaac gctatgatgg acgaattgaa
     7741 acatcgacta tccgtattgc cggaagagtt cgacaaagat aaaacgctgt tcaacacaca
     7801 aagcggctat ctgtctttgc atgacggtct tctacacgag catgagatag acaaaatgtt
     7861 tacacgtgtt tcaaatgtcg agtacacaga atcggtcgat tgtcctatgt gggaagaatt
     7921 tattaatcag atttttgaca atgatcaaga attaatcaga tatatccaga aatgtgtcgg
     7981 atattccctg accggatcta ccagagagca atgtatgttc atcctctacg gtcatgggtc
     8041 aaatggaaag tcggtatttt tggaaattat ttctgaattg atgggaaact atgcgatgac
     8101 tatgcaggca caaacgatca tggtcaagca aagccaatca tcagcaaaca gtgatattgc
     8161 acgattgaaa ggtgcgcggt tggtcacttc gtcagaaccg aatgaaggcg tccgactgga
     8221 cgaaggtctg gtcaaacaat tgactggtgg tgataaagtc acggctcggc acctttatgg
     8281 aaaagaattc gagttcgaac ctgaattcaa gttgtggctg gcaacgaacc acaagcccat
     8341 catccgtggg accgatgacg gcatctggcg acggttaaat ttaattccat tcactgtcca
     8401 aatcccggat cacaaaaagg ataaaaatct gaaatttaaa ttgcagacgg agttgcaagg
     8461 gatccttaaa tgggcaatcg atggttgcct gttgtggcag agagagggct tggagaaacc
     8521 aaagtcggtt gttgcagcga gtcaggatta tcgaaacgaa atggatcaaa taggtacatt
     8581 tatcgaaacg tgttgcgaaa caggtccagg actaaaaata tcaggcggag aattatacaa
     8641 agtttatcgt gaatgggcat cagataatgg cgagcatact ttcactaaca cgaagtttgg
     8701 cagagagatt tcaaagaaat atagcaaaga aaaagctggt ggatttatgg tttataaagg
     8761 aatcacatta aaaccaagaa aatacgataa cgttagagaa ctttttaaat aaatctggag
     8821 ggttctggag ggttgagaac gaaaccctcc aacccttaga aacg
//
LOCUS       DAZKOM010000109         8679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000109 DAZKOM010000000
VERSION     DAZKOM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.112"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..404
                     /locus_tag="ACG8NF_002469"
                     /pseudo
     CDS             204..404
                     /locus_tag="ACG8NF_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1116..1721)
                     /locus_tag="ACG8NF_002470"
     CDS             complement(1116..1721)
                     /locus_tag="ACG8NF_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0472899.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            1903..2238
                     /locus_tag="ACG8NF_002471"
                     /pseudo
     CDS             1903..2238
                     /locus_tag="ACG8NF_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2403..2618
                     /locus_tag="ACG8NF_002472"
                     /pseudo
     CDS             2403..2618
                     /locus_tag="ACG8NF_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2756..3727
                     /locus_tag="ACG8NF_002473"
     CDS             2756..3727
                     /locus_tag="ACG8NF_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0472900.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            3867..5180
                     /locus_tag="ACG8NF_002474"
     CDS             3867..5180
                     /locus_tag="ACG8NF_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0472901.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5223..6089
                     /locus_tag="ACG8NF_002475"
     CDS             5223..6089
                     /locus_tag="ACG8NF_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472902.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6086..6934
                     /locus_tag="ACG8NF_002476"
     CDS             6086..6934
                     /locus_tag="ACG8NF_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472903.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            6948..8420
                     /locus_tag="ACG8NF_002477"
     CDS             6948..8420
                     /locus_tag="ACG8NF_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0472904.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8437..>8679
                     /locus_tag="ACG8NF_002478"
     CDS             8437..>8679
                     /locus_tag="ACG8NF_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0472905.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2736 a         1489 c         1785 g         2669 t
ORIGIN      
        1 gagaacacca cgcatagttt ttattttgag atattctaga atgaaacaca ttaagaacga
       61 aagcaaagca aaagacgcca gaacttctac taccttctaa gttttttcat atttgtttgt
      121 tatgctatac atagatctgc ataattagac ttgcgctgcc cgttattgaa gtttcaggca
      181 gcgctctttt tgggggatcg ataatggaag aagaaaaaat atttgagaaa cgctggcagc
      241 tagcatcaag tgaacaaaga gcaagataca ataatttgat cacctcctac cccactgttg
      301 actagacata taaggaaaag aaatacttac tttggctgtg ccagttagat atagatacct
      361 ttgaaacatt tgaagtgatc ctagataaaa ttaaacagaa ttgatcagaa acaagcgaag
      421 ccttctctcg gttcgcttgt tttgctattt attagttggc tcagggcaac tctaagctcg
      481 tttgaaagaa aaccgatgga taatcccctt gtttgtacca ctttagaata catggatgta
      541 cgtattgcca ttgactccta ttagttactg aaaaaaaatt taaacaagtt gagtattgca
      601 gataaatagc tccagtgagg tgaataactg ctccactcaa ggcaatagat agctccatcc
      661 agggcaacaa actgctccac tttcatattt aatagatgaa taaccttctg tataatagaa
      721 aggcatacct ttcggtatga caacttatac agaatgttat tttattggtt cactatcgaa
      781 aaattttgga gctttatttt tccggtattt ctcaacgaac gattagttta actgtgggaa
      841 gctccagaca tacgattcga gaagttactt ctcgagcaga caggaagggg gtgactgaac
      901 taaccgctga tatgagcgat acttggctgt aggaatatct ttttccggaa aaacaaacaa
      961 cagcacgagg ttaccttcct gtagactggg aatatgttca taaagaacta atgaaaaaga
     1021 atatgacatt gaaactcctt catagagaat atggggtaca tggaacatag aaacgaaaat
     1081 gcacggtaga aaagcgaatg aatatagtat aaattctaca aatcattcgc tttctttatt
     1141 ttatatatgg ttggtcgaga gagaccattt tctttggcaa tttctgcaac tggaactcct
     1201 tgatttaaca tgtccaccac agttttaaag actaatcgtt tttgcggatc tgttgagttc
     1261 tcagaatatt caattggtcg ccccttatat cgaccagctt ttttggccaa taatactcct
     1321 tgccgctgac gttcacggat tttctcacgt tcgttttgtg caatataact tagcaatgat
     1381 aaaaacatat cgaacatagc agtatctagg agttcatttc cagtattaaa atctaaaaac
     1441 tgtgcatcta gtatttttaa tgctactttt ttctgtttca taaaggtaac tgtatttttt
     1501 atgtcttcat aatctctgcc taggcgatcc agtgatgcaa caatgacaca atcaccttct
     1561 cggatataat ttagtaattt ttgaaactca ggtcgctgga tatctttacc actggctttt
     1621 tcttggaata atttatcaat gttaaggtct tccatcattg ccaattgtcg atctaaattt
     1681 tgatcttgtg aagacactct aatatatcca atttttgcca taaaaactcc ttttaaaaaa
     1741 tgatataatt tacatgtaaa atttacttgt agttttactg ttatcatagc atttttaaaa
     1801 ataaatttta agtctactta taatttactt tggaggtttt gaatgaatga aattaagcgc
     1861 aataaaaatt cttttggtgt ttatttagga gagcctagct cggaacagtt aaataaaggt
     1921 gaagctcgtc atgctctatg tcgaaatata ttttatggta gaaaaggcaa actttatcaa
     1981 acttattacg acggtatgga ggaacagctc aacgcattag gattagtaac taatgctatc
     2041 atatattgga attcactata tttagaaaaa gtctttgaac aaattaaaac ggaagggttc
     2101 gactgttccg aagaagtgtt acgcaagcaa tttccattaa ttagtgatca cattaatttt
     2161 gttggcaaat atagtttccg ctatgaaaaa gaattatcga atggaatact acgtccgtta
     2221 aatatagaga attattgaga atgttcgtct tttggttcct accgtgcatt ttcgcacgga
     2281 tgttccagat actatttttg aatgtataaa attgaaaaca caagaatact aataaatcaa
     2341 cgattcttgt gtttttaatt tttcactacc gtgcattttc gtttctatgt tccatgtacc
     2401 ccaatcttga aaaattaatt aaagacttag ataaagccat tgacgactta gtcgtggtgc
     2461 taccggagta tcaatgttta accagtatcc caggtattgg tcctgtttag gctgctggaa
     2521 tactcgctga aattggtcca attgaacgtt tttctgatga aataaaattg acaaagtatg
     2581 ccggcctata ttggcgacag agtcaatccg gtaaatctga atacgaaaat ttttttacga
     2641 aaagcttcac gtctaatatc tattttaaaa taaaaatatt cctgctaatt tggaatcgtt
     2701 tccttctttt ggtataataa tacctgaaat tcttttgaga ggagttgttc gtgaagtgaa
     2761 aactactatg aaggatgttg ctgcgttagc aggagttggt gtgggtacgg tatctagagt
     2821 aataaatgga gtaaaggtga aagaggttac caaagaaaaa gtaatggctg caattagtga
     2881 gcttaattat gagcctgatg aatatgccag aggtatgaaa acgaatagta gcaatacaat
     2941 agctcttatt ttacctacga tttggcatcc cttcttttcc gagtttgcct actatgtaga
     3001 ggaagctctc agccaaaaaa attataagct ttttttgtgt aatgcgaacg gcagtgcgga
     3061 aaaagaaaaa gaatatatcc aaatggtcaa gcagagtaaa gtagacggaa ttataggtat
     3121 tacctattca gacattgata aatatgtatc atcaaattta ccatttgtca gtatagaccg
     3181 ccatttttcg gaacaggttt cttatgtaac ggcagataat tatttaggtg ggcaaattgc
     3241 cgctcaagaa ctacttactc gtaactgtaa aaacttgtgc tatgttgggg gtgtttcacc
     3301 ttaccctaac gaaacaaaca atcgtaaagc tggtttttac aatatttgtg aggaaaataa
     3361 aatagctgtg aaggtattgg atatgcctga accaattagc aatttagagg aacaattgct
     3421 cgcttttttt tcggataatc ctgaaattga tagcattttt accatcaatg atttcatggc
     3481 tttggatgtg ataaaagccc aaaaaaaact agggaaggaa gcacctaagg attttcaaat
     3541 tattggtttt gatggagtca agctatcgtt gcaaaaagac tatttactat caacgattgt
     3601 acagccgata gaggaaatgg ccaaggtttc agtagaaaca ctcatggcga tgattttaaa
     3661 tgaagaaccg cctaagcgtg tggtagtacc ggttttcttt cacgctggag agacgactaa
     3721 agagtaaaaa accggtttat aacgattttt ttgagatagg ttgacagaaa gtgatttcaa
     3781 tgatatctta aaaccaatta attggaaacg ttaccaatta attgaaaaat aaaacggaaa
     3841 cgtttcaaaa aataggagga acatcgatga aaaagagaat gttagcaatt gcagcaattt
     3901 tagcattaac agctggagga cttgcaggat gtggcagtaa ggagaaaagt tcaggtagca
     3961 gtgatccagg agaaattaag atttgggttc agttttctga tgaaacgcct gaaggaaaag
     4021 cttgggcaga gattgtgaac aattttaatg atgaatacga gggtgaatat aaagctgtta
     4081 ctgagtatat tcctcgtagc ggaagcggcg gtggatatga ggataaagta aatgctgcaa
     4141 ttacaaccaa tacccttcct gatgttatta cgttagatgg accgaatact gcagcttatg
     4201 ctcattcagg agtcattgct ccattagatg attatttgaa agatgcggac atggatgatg
     4261 tattagacag cattattcag caaggaacct acgatggcaa aatgtatgcg tttggtttct
     4321 cagagtccag tgtgggtgtc tattacaata agtcgctgtt caaagatgct ggaattacag
     4381 aagatcaatt gccgacgtta gaagatcctt ggacttggac ggaatttcaa gcaatttgcc
     4441 aaaagctgac tgataaatac aaaacgcctg caattgatat gcaaatggcg tcgggagacg
     4501 aaatgctgac ctacgcatat acaccattta tatggtcgaa tggcggcgat gtcgttaatg
     4561 ctgatggaac caaagccgaa gactatttta acggtaaaaa atccgttgaa gcgttggaat
     4621 ttattcaaga attggttgaa aaaggttaca ccacaatcag tcctattgaa aaaggatttg
     4681 aaactggcaa atatgccatg atgctgagcg gttcttggac aatggcagat ttagaggaaa
     4741 actatcaaga gattgattat ggtattttgc cttaccccgt ttcagatgca actggggagt
     4801 tagtttcacc aaccggaagc tggcagttag caatgactga gaaaacagag aaaaaggatg
     4861 cttctgcggc ctttattaag tttgcaacca gtacagaatc tagtcgtatt atgagtttag
     4921 ggaacagtgt tctacctatc agaaattcaa cagttgattt aattaaagat gaagtgtcgg
     4981 aaggaatgca ggtattaatt aagcaaaacc aactttctgg acatgcccgc ccagtggttg
     5041 tagcttatcc gcaagtatct agagcattcc aacaagcagt tcaagatatt agctacttcg
     5101 aggaaaaccc agatgtacaa aaagtagcag atagtcgcgc tgctgaaatg caacaggcaa
     5161 ttgatacttc actaaaatag aagtttctaa agggggccaa tcaagtgtta aaaaaaccga
     5221 caatgaaaga caatttgata ggttacggct ttctagcgcc agctttgctg ctgctgacga
     5281 tttttctgat catacccgta atcatggtaa tttattatgc ctttactgat tattatttgt
     5341 taacgccaga tgctagacaa tttatcggct tagaaaattt taagcgactg ttcgctgatc
     5401 caatttttgt aaaaagtatt tggaacacgg ctaaatttgt tgtttggatt attccggtcc
     5461 aaataggggc agctttagga atggcactta ttatcaacaa aaatcgtaaa ggaaatatct
     5521 tttttaaagt agcctttttt gcgccagtcg tgatgtcctt agtcgttatt tcgattttat
     5581 ggttgtactt gttaaatcct aatgaaggtc tggttaatac gctatttgca aagattggaa
     5641 ttgcttcgca gccttttttg actagtccca aacaagcaat gtatacgctt gttttcgtat
     5701 cggcatggca aggtgcagga tatcagatgc tgctgttttt agggggaatg caaaatattc
     5761 cgcaagacgt ttatgaggca gccgaaattg atggtttttc taagtttcag caatttcgct
     5821 atatcaccat gccattatta aagcccacgg cgatttttat tttacttacc acattgattt
     5881 cggcctttaa actaatcatt cagccaatgg taatgactca aggtggacca atgaattcta
     5941 ctatgacaat ggtttattac atttaccaaa ctggatttac agaccgtatg gtaggctact
     6001 ccagctccat tgcgttaatc tttaccacta tgattggttt aattacaatt gctcaaagaa
     6061 aattggtaaa ggaggatgat ccagcatgac caagaaattt ggaccgctaa tgatcgtaga
     6121 atattgtttg ctgttccttt tagcggcatt attcattttc ccgatgctgt ggatggtagt
     6181 ttcctctatg aagcctgaag cagatgtcta tcaaaattta gcttccttca aagctttttt
     6241 acctagtttg aatccagcta attggtttaa aacttatcaa gaggttattt cccggttcag
     6301 tgtgggaact tatttattaa acagtatttt ttatggttta acctttgcct gtgggtcaat
     6361 cattgtaaac tctttggcgg gttttgcctt tgcgaaggtt aatttttcag gcaagaaaat
     6421 tatctttgga gctttgttag ctctgctgat tgtcccgatg gagacggttt taattccgca
     6481 atttaccatc gttaattcac taggcttagt gaatactcgc ttggcagtga ttttaccagc
     6541 gatggccagc gcgtttaata tctatctgtt tagaaatttc tttatcgcga ttccagaaga
     6601 aatcattgaa tcagcgaaat tagatggtgc aaatattgtt aaaattttct ttcacatcat
     6661 gctgccaatg tctaaaccag ccatagccac agtaggtgtc ttatccttta tctccagctg
     6721 gaatgattat atttggccgt taatggtctt aactgacagc agcaaatttt caatgcaagt
     6781 ggcaattact acgatcaata ctacacagcc agtttatatc aatcaagtga tggctgtttt
     6841 aaccattgca acgattccac tgattattgt ttatatcgtt gcacaaaaat atattctgca
     6901 gggacttggc ggttctggaa cgggtataaa ataggagtga atagtgaatg ttgcaaaaac
     6961 ttttaaaggc caatgagttt attgagaaaa atgctcaaac agtcaatcaa gaataccgga
     7021 ataaatttca tttgatggct ccgattggct ggatgaatga tccaaatggt tttgtttatt
     7081 atcaagagga gtatcacttg ttttatcaat attatcctta cgacagcatc tggggaccaa
     7141 tgcattgggg ccatgctaaa agcaaggatc taattcactg ggagcatttg ccagtagcac
     7201 tggcaccagg tgaaagctat gacaaggaag gctgtttttc cggcagtgcc atagaaaagg
     7261 atggcaagct gtatttgatt tatacaggac acaatgttgt ggaagggcaa gttcgacaag
     7321 ttcagtgttt ggcagtttca gaagatggca ttcactttga aaaatatgcc ggaaatccaa
     7381 tcatcgcaga agatcactta gcttctgttg caacgaccga agatttccgc gatcccaaag
     7441 tgtttcagcg aaaagaagac tattttactg tagtcgctac taaaactgca gatgctcgtg
     7501 gacaaattgt gatgttccat tccaaagatt tactaacttg ggagttttat tccgtattgc
     7561 tggagggaac tccagaacaa ggcattgtat gggaatgccc agacttgttt gaactagatg
     7621 gcaaagaagt cttgattctt tctcctattc aaatgactcc acaaggcaat gcttaccaaa
     7681 atacgaactc aactgttgct ttcatcggta ctgtcgattg gcaaacaggc aaatttctag
     7741 tagagaattt tcatgaaatc gatggcggtt tagattttta tgcaccccag acctgtgaaa
     7801 acgaagcagg ccaaagaata atgattgctt ggatgcaaat gtggcagcgt aaaataccta
     7861 cgaatgatct gggccatggt tggtcaggtt ccatgacctt gcctagagag ctgagggtgg
     7921 ctgataatca attgttgcaa cggccagttt catcaattta tcaagtaatt gatccgcagg
     7981 aaaggattga atgggcgaag actgaagaga atcgcttctt actgaaggac ttagtatatg
     8041 accagcacta tttacgcttg gaaatcaatt taaaacaggc aacgaaacta gaacttcact
     8101 atgcgaaggg acccgaccat acattactgc ttaactatga tgttgaacgg gagtgcttta
     8161 ctgcttccag ggaacacatg ccgtatgatt tatcaggagc tgaaaaggaa gtgttgaaaa
     8221 agcgcagtgt gacgattcct ttggaaaata attgtctaac catcgaaatt ttccgtgata
     8281 cttcagctat tgagcttttt ctaaatgatc gtctgactat gagtacaact ttttatgaga
     8341 cagaaaaagg acaggatttt cttttgctgg cagacagtga agtaaccttt tcattggaaa
     8401 gcggcttaat taatctatag aagggagagc tcagtgatga tttatggttc aatcgaagca
     8461 ggcggcacaa aatttgtgtg tgccattagc gatgaagaaa tgacgatcaa ggaaagagtg
     8521 agctttccga caacgacacc tgaggaaaca atacctttag tcattgactt cttcaaacaa
     8581 taccaagcag atttagcagg gattgggatt ggctcatttg ggcctatcga tatccatcgc
     8641 gattccgcca cttatgggta cattacctcg accccgaaa
//
LOCUS       DAZKOM010000110         8673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000110 DAZKOM010000000
VERSION     DAZKOM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.113"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(271..2652)
                     /locus_tag="ACG8NF_002479"
     CDS             complement(271..2652)
                     /locus_tag="ACG8NF_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0472906.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2642..3289)
                     /gene="recU"
                     /locus_tag="ACG8NF_002480"
     CDS             complement(2642..3289)
                     /gene="recU"
                     /locus_tag="ACG8NF_002480"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0472907.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3353..3892
                     /locus_tag="ACG8NF_002481"
     CDS             3353..3892
                     /locus_tag="ACG8NF_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0472908.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3982..4401
                     /gene="gpsB"
                     /locus_tag="ACG8NF_002482"
     CDS             3982..4401
                     /gene="gpsB"
                     /locus_tag="ACG8NF_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0472909.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4423..4790
                     /gene="rnpB"
                     /locus_tag="ACG8NF_002483"
     ncRNA           4423..4790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8NF_002483"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4999..6165
                     /locus_tag="ACG8NF_002484"
     CDS             4999..6165
                     /locus_tag="ACG8NF_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0472910.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6211..7707
                     /locus_tag="ACG8NF_002485"
     CDS             6211..7707
                     /locus_tag="ACG8NF_002485"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0472911.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7788..8471
                     /locus_tag="ACG8NF_002486"
     CDS             7788..8471
                     /locus_tag="ACG8NF_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0472912.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8480..>8673
                     /locus_tag="ACG8NF_002487"
     CDS             8480..>8673
                     /locus_tag="ACG8NF_002487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472913.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2728 a         1572 c         1843 g         2530 t
ORIGIN      
        1 caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa
       61 ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat
      121 ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt
      181 aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa
      241 accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct
      301 ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc
      361 aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt
      421 tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga
      481 ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg
      541 agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc
      601 tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc
      661 ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga
      721 ggctacgtgt gacgattctg aagtgacagg cgtcattttt ttattgtaac ctgtccacac
      781 ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga
      841 aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg
      901 atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat
      961 gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg gctcataagt
     1021 ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc
     1081 tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt
     1141 cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc
     1201 gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt
     1261 gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata
     1321 ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt
     1381 aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg
     1441 tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac
     1501 gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat
     1561 aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac
     1621 gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc
     1681 tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt
     1741 atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg
     1801 tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata
     1861 caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga
     1921 aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt
     1981 cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac
     2041 tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc
     2101 catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa
     2161 ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa
     2221 gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg
     2281 gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat
     2341 tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt
     2401 ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata
     2461 ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag
     2521 gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga
     2581 attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg
     2641 ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg
     2701 cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga
     2761 tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca
     2821 aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc
     2881 agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt
     2941 taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc
     3001 gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag
     3061 cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga
     3121 taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc
     3181 gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg
     3241 gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca
     3301 ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa
     3361 gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa
     3421 tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga
     3481 agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca
     3541 aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt
     3601 ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca
     3661 attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa
     3721 gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga
     3781 attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc
     3841 ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt
     3901 gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa
     3961 atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa
     4021 caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac
     4081 aatatcatca aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac
     4141 gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc
     4201 gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc
     4261 ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc
     4321 aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat
     4381 gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg
     4441 tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt
     4501 cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta
     4561 aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa
     4621 cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc
     4681 tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg
     4741 ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac
     4801 atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa
     4861 agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc
     4921 ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta
     4981 taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca
     5041 agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt
     5101 gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg
     5161 ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat
     5221 gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct
     5281 tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg
     5341 atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta
     5401 ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg
     5461 atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa
     5521 ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga
     5581 aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc
     5641 ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat
     5701 tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat
     5761 gatattgaat tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa
     5821 gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa
     5881 gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt
     5941 ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca
     6001 ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga
     6061 caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag
     6121 tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt
     6181 cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta
     6241 aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt
     6301 atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat
     6361 ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg
     6421 gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg
     6481 gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac
     6541 ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat
     6601 caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat
     6661 tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta
     6721 tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact
     6781 gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta
     6841 gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc
     6901 cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa
     6961 acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa
     7021 caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc
     7081 catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa
     7141 aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac
     7201 aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt
     7261 ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa
     7321 ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga
     7381 atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc
     7441 tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct
     7501 ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa
     7561 aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc
     7621 atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg
     7681 tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct
     7741 aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac
     7801 aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg
     7861 atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag
     7921 accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta
     7981 aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag
     8041 aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct
     8101 ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag
     8161 cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga
     8221 tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca
     8281 cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg
     8341 attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat
     8401 tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata
     8461 acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt
     8521 ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc
     8581 cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg
     8641 tgattcggaa ttggcaggag aacaccgttt att
//
LOCUS       DAZKOM010000111         8634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000111 DAZKOM010000000
VERSION     DAZKOM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.114"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..495)
                     /locus_tag="ACG8NF_002488"
     CDS             complement(49..495)
                     /locus_tag="ACG8NF_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0472914.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(522..698)
                     /gene="rpsU"
                     /locus_tag="ACG8NF_002489"
     CDS             complement(522..698)
                     /gene="rpsU"
                     /locus_tag="ACG8NF_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0472915.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(860..1288)
                     /locus_tag="ACG8NF_002490"
     CDS             complement(860..1288)
                     /locus_tag="ACG8NF_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0472916.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1380..2240
                     /locus_tag="ACG8NF_002491"
     CDS             1380..2240
                     /locus_tag="ACG8NF_002491"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0472917.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(2334..2684)
                     /locus_tag="ACG8NF_002492"
     CDS             complement(2334..2684)
                     /locus_tag="ACG8NF_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0472918.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2700..4427)
                     /locus_tag="ACG8NF_002493"
     CDS             complement(2700..4427)
                     /locus_tag="ACG8NF_002493"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0472919.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="ACG8NF_002494"
     CDS             complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="ACG8NF_002494"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0472920.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5711..7144)
                     /locus_tag="ACG8NF_002495"
     CDS             complement(5711..7144)
                     /locus_tag="ACG8NF_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0472921.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7144..8547)
                     /locus_tag="ACG8NF_002496"
     CDS             complement(7144..8547)
                     /locus_tag="ACG8NF_002496"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0472922.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         2329 a         1942 c         1485 g         2878 t
ORIGIN      
        1 gaatttcttc accaacactc attgacgaag agcctttaaa tgataaaatt aagattgtaa
       61 tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat
      121 gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc
      181 ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac
      241 tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc
      301 atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc
      361 tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat
      421 gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct
      481 acttagtagt gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt
      541 ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta
      601 cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg
      661 tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct
      721 taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat
      781 attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa
      841 tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat
      901 gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga
      961 tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt
     1021 gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga
     1081 tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct
     1141 gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag
     1201 ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg
     1261 attgttcaat caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa
     1321 gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca
     1381 tgtcttccga ttctacagaa atcattactt tttttgtcat ctctgactct gctggagaaa
     1441 ctgcgacaaa attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat
     1501 tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa
     1561 aagaaaaagc ccttgtccta cacacattga tcaatcaaga attgatcgag atgacgcgtt
     1621 ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa
     1681 tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg
     1741 agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag
     1801 atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa
     1861 cgcctttgag cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc
     1921 cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga
     1981 cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa
     2041 atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg
     2101 atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa
     2161 cagcttcact tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa
     2221 ccaccgaaga aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt
     2281 cagcgaagaa gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga
     2341 tgttccacat gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
     2401 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
     2461 agttgatgcg atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt
     2521 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
     2581 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
     2641 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
     2701 tattcttctg tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct
     2761 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
     2821 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
     2881 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
     2941 tggatgactt ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa
     3001 gcagagattt ttgcagagcc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
     3061 tagtagccat atgtttcaaa aatcgtttgc aagccgtcat acaatgtttt gccttgtttt
     3121 ttatagtaag ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga
     3181 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
     3241 tgatcttctt caaattgttg aattttttca gcaatgaatt tgaatcctgt caatacattg
     3301 aacatggtag ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat
     3361 ttcaataccg ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
     3421 tgagcaaaca gggctccgat ttggtttcct gtcaatactt cataggagcc atttggcaaa
     3481 cgaacggctg cgcctaaacg gtctgcatca ggatccgttg caatcagtaa gtcagcgcct
     3541 tctttctcgc caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat
     3601 ttgactgtag tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
     3661 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
     3721 tacacaagct tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct
     3781 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
     3841 tgttcagctt cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
     3901 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
     3961 ttatatccgt tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc
     4021 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa
     4081 gaagggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
     4141 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc
     4201 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
     4261 ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
     4321 tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa gttttcttct
     4381 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
     4441 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtatgag
     4501 gaccaaaaaa cgcttaaatc aagaaaagat tcttttatca agcacggtta caagtattcg
     4561 cataaatcgg attcagtaga tttcacgcaa aaaaaagagt aaaagaacaa ttgaaaccgt
     4621 tctttcactc tttttaaact tatgcagttg tttcttctaa agattccaag tatttataca
     4681 cttgttgtcc agcaatccca ccatcaccaa cagcagtagt gatttgtcgc aaatcttttt
     4741 cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg ttgtctgttg
     4801 gaatccagcc ggcttcgttc gtcaacccag cttctctaaa tggtgcagtt aatggatcca
     4861 atcctacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgcca gttttgacat
     4921 tacgtgcttt cacacctgtc actaccattt catttccaac gatttcgtca acgactgtat
     4981 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
     5041 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
     5101 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaatg
     5161 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
     5221 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
     5281 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
     5341 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
     5401 ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc atttggccgc
     5461 caggagcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
     5521 cagtcatccc agcaggccct gcgccaataa cgattacatc atacatatgt agattctcct
     5581 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
     5641 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
     5701 aaatcattga tcaatcgttc gaaagatttt ctccatttga atcaattact tttttatacc
     5761 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
     5821 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
     5881 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
     5941 cctggatatg tgctgccaga tagtcgattc gataatcatc ttccacataa ccattctcat
     6001 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
     6061 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
     6121 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
     6181 gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata aaatcaacag
     6241 tatgttcttt cagcaatgcg gcatccccag gttccatttt gatctgaatg ccatttcgtt
     6301 ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca atgaagaaat
     6361 aattttctcg atctgacttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
     6421 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcga
     6481 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
     6541 cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca tgaaggatca
     6601 tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga ttgaagatga
     6661 ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
     6721 tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg atcccgtact
     6781 tttgacattc tttaaagaca tcctcataaa atttcaaccc agattcattt ggctcttttt
     6841 catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc ttgaacccca
     6901 tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc gcaatttttg
     6961 ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta atgacaggaa
     7021 aacggtcttc tccgattggc acaacatcta cgttcgctaa cccacgtccg ccttcatcat
     7081 aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct tttctgaatc
     7141 ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca gttcatcttg
     7201 gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg gtaaatactg
     7261 ctgatgtgca ataaacaact catccatgat ccgttttgct gtctttcctg atcgtataag
     7321 cggattgatc gtaaacgctt gcaaagctgt tccataatca ccagtcacgg ctgcttcgat
     7381 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaaggga
     7441 accaaacgct acatttcgtg cgccttgtgc gccaacatag gcagtgactt ctactacaca
     7501 atctgccggc agatcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
     7561 tttatttcca tagatcgaag caatcgtttc acaggcagca tctgaataat aagcaccacc
     7621 tcgtttttgc aattgttcag gtttatgatc caattctgga tctttgtata attcaaacag
     7681 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
     7741 agcatgttcc agcatttctt cttcacgata gtaatagcgg tggtagccac aagggatcat
     7801 gttcatttgt tttaattgtt ctttgaaaaa cgggatgtca aagatatttt tcggcaatcc
     7861 attgtcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
     7921 tgctactttg tgccaatgga agtgatttag accggcaaac ttgtagatca gctcttcttt
     7981 tttcttgcca agcattgttg gttctgtcat catcgccatt actggcacat tgcataatcc
     8041 gatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cgcttgggtt
     8101 cgcgaaatta atcagccaag catttggaca taaacgcttc atgtcttcaa cgattgccaa
     8161 aaccactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
     8221 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
     8281 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
     8341 atagattttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactgacgat
     8401 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
     8461 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
     8521 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttac acttttatta
     8581 tcttttttgt aaacaaaaca aacaatagaa attcgtttct ttccagtaac attt
//
LOCUS       DAZKOM010000112         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000112 DAZKOM010000000
VERSION     DAZKOM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.115"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8NF_002497"
     CDS             complement(<1..149)
                     /locus_tag="ACG8NF_002497"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0472923.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8NF_002498"
     CDS             complement(593..1714)
                     /locus_tag="ACG8NF_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0472924.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8NF_002499"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8NF_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0472925.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8NF_002500"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8NF_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0472926.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8NF_002501"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8NF_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0472927.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8NF_002502"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8NF_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0472928.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8NF_002503"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8NF_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0472929.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8NF_002504"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8NF_002504"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472930.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKOM010000113         8079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000113 DAZKOM010000000
VERSION     DAZKOM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.116"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(510..1076)
                     /locus_tag="ACG8NF_002505"
     CDS             complement(510..1076)
                     /locus_tag="ACG8NF_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0472931.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="ACG8NF_002506"
     CDS             complement(1076..2032)
                     /locus_tag="ACG8NF_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0472932.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="ACG8NF_002507"
     CDS             complement(2032..2688)
                     /locus_tag="ACG8NF_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0472933.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="ACG8NF_002508"
     CDS             3058..5076
                     /locus_tag="ACG8NF_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0472934.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /gene="leuS"
                     /locus_tag="ACG8NF_002509"
     CDS             5503..7917
                     /gene="leuS"
                     /locus_tag="ACG8NF_002509"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0472935.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         2685 a         1527 c         1619 g         2248 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttctt
//
LOCUS       DAZKOM010000114         8023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000114 DAZKOM010000000
VERSION     DAZKOM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.118"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1797
                     /locus_tag="ACG8NF_002510"
     CDS             259..1797
                     /locus_tag="ACG8NF_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0472936.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1794..2759
                     /locus_tag="ACG8NF_002511"
     CDS             1794..2759
                     /locus_tag="ACG8NF_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472937.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2746..4068
                     /locus_tag="ACG8NF_002512"
     CDS             2746..4068
                     /locus_tag="ACG8NF_002512"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0472938.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4131..4235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4309..5052
                     /locus_tag="ACG8NF_002513"
     CDS             4309..5052
                     /locus_tag="ACG8NF_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0472939.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5067..6059
                     /locus_tag="ACG8NF_002514"
     CDS             5067..6059
                     /locus_tag="ACG8NF_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0472940.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6071..6808
                     /locus_tag="ACG8NF_002515"
     CDS             6071..6808
                     /locus_tag="ACG8NF_002515"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0472941.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6798..7520
                     /locus_tag="ACG8NF_002516"
     CDS             6798..7520
                     /locus_tag="ACG8NF_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0472942.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7604..>8023)
                     /locus_tag="ACG8NF_002517"
     CDS             complement(7604..>8023)
                     /locus_tag="ACG8NF_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0472943.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2570 a         1389 c         1751 g         2313 t
ORIGIN      
        1 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
       61 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      121 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      181 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      241 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      301 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      361 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      421 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      481 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      541 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      601 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      661 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      721 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      781 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      841 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
      901 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
      961 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1021 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1081 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1141 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1201 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1261 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1321 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1381 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1441 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1501 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1561 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1621 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1681 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1741 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1801 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1861 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     1921 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     1981 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2041 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2101 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2161 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2221 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2281 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2341 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2401 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2461 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2521 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2581 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2641 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2701 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2761 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2821 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     2881 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     2941 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3001 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3061 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3121 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3181 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3241 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3301 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3361 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3421 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3481 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3541 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3601 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3661 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3721 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3781 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3841 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     3901 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     3961 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4021 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4081 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4141 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4201 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4261 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4321 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4381 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4441 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4501 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4561 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4621 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4681 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4741 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4801 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4861 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     4921 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     4981 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5041 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5101 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5161 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5221 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5281 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5341 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5401 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5461 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5521 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5581 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5641 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5701 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5761 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5821 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     5881 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     5941 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6001 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6061 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6121 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6181 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6241 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6301 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6361 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6421 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6481 cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc
     6541 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6601 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6661 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6721 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6781 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6841 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     6901 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     6961 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7021 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7081 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7141 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7201 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7261 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7321 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7381 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7441 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7501 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7561 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     7621 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     7681 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     7741 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     7801 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     7861 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     7921 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     7981 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZKOM010000115         7750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000115 DAZKOM010000000
VERSION     DAZKOM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.119"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..2758)
                     /locus_tag="ACG8NF_002518"
     CDS             complement(56..2758)
                     /locus_tag="ACG8NF_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0472944.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2771..4060)
                     /locus_tag="ACG8NF_002519"
     CDS             complement(2771..4060)
                     /locus_tag="ACG8NF_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0472945.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4210..5256
                     /locus_tag="ACG8NF_002520"
     CDS             4210..5256
                     /locus_tag="ACG8NF_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0472946.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5258..7411
                     /locus_tag="ACG8NF_002521"
     CDS             5258..7411
                     /locus_tag="ACG8NF_002521"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0472947.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2181 a         1579 c         1412 g         2578 t
ORIGIN      
        1 tctaaaattt ctccaccatc tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt
       61 ccgccataca ttcgttttcc tcctcgaatc cgattgtccg aatctcataa ggttggatta
      121 ttgggacaac cgcttgcttc gttggttctt caagcagatt caatatgaca gtttttccat
      181 tgtctttagt tatttccaat ttttctaaat gactagacat attgaaacct cttactacta
      241 ctttatcact aaacttgctg cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg
      301 ttaaatattg ttgttttgaa atcaatgttc cttcatgatg tttgatttgt tgagtggaaa
      361 aaggaatctg tgctgcgtat gcatgcaaat aagtagagaa tttttcttct ggtccatgta
      421 gttcaatgct gtaattgaac cgatgctcac ctagacactg tgcttctggt gttggaaaat
      481 atccccaatc gcctagttct cccacactgc gaagtagcgt cactgcgatt tgtccgtctt
      541 ctgttacttc atattcattc aaaccaaagt ttccgactgt tacaccgtac tcttcatcat
      601 gaatattgac aaatgcttgc tgatgttgcg gattcgtggg gttttcccaa cttttagaca
      661 catggtttgg gcgcttcacc acttcaaaaa tactatctgc ttcgtgatgt tccacatgta
      721 actttgtagg aaacaagaca cgtaagcgat gatctttcat ctgattgtcg agagtcgttt
      781 caaagtcgat ttttttacta tcttttctga ttgtcatgat tgtttttatt tgaaggattc
      841 gtttttcttt ggatcgttct gcttttcgat aacgaaattc cacaaccatc tgctgttctt
      901 tttctaaacg ctcgtctgca gaaacaggga tttccatttc ctgtagaata cttatttttg
      961 ctatttccgg tgtatctact aatactttta cttcagagtt ttctttgtta ctggatagga
     1021 ttggctgatc acagaaaggt tgtttaaaga tatactcatt tcctatatcg ccgatatctt
     1081 caaaaatatg cagattattc aactttcgtt tgtttttgcg atcttccatt gtaatggtac
     1141 catttttttc aatcgtcaaa tgtagagggc cgttttcgat gattcgtcca ctctgatgaa
     1201 tcattgtttc cttttcttct gctttcgctt ccccttctac aagcgcgaat gtttcccaag
     1261 aaaatgctgg catgttttct aatggtaaag tcactttgat cattcttgcc atatatggca
     1321 cacggaaacg gtctttcggc aaatcataac caaattggac agtttcttca gataaaatag
     1381 ctggtatagt ggccccactt tttgtttcta catgatactt acgtttaggc aaattttcta
     1441 actcttgata tagttgttca ggtattcctt cagcgaatct ttttctttct aattcgatgg
     1501 ttatttctgc ttcaccagtt ttatccattc ctgcagtatt tacaatgaca aatggaaagc
     1561 tgtcttttgg gaatccggtc gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg
     1621 caagatactt tccaacttca tctgcttttt caaaacgagg tatcatttcc cgatggacag
     1681 aatcgactga acaaccgcaa atactgtcgt gaggatgatt ttgcattaat gtcttccaag
     1741 cataatctaa ttgatcatgg ggatagtttc cactaacttc ataagccatc gtcgcaagtg
     1801 gttctgtaat attttctagc tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc
     1861 gagcagatgc tgtattggct aaagtatacc agccatccgt ttcttgactt gtcaattctc
     1921 cttctactga gcctaagtct tctggcacat ctttttgaac tgcttccaga tagtcagtaa
     1981 aattagaatg aataaactcg taatctggaa acagttcatt tgcgagatgg attgcttttg
     2041 atatatcttt ttgtaccggt tgatgatcaa caccattcat catcaataag tggttcgtag
     2101 aggcatattg ttctgcatcc gctaattttt gcttccaaaa ggcaagagct gcttcttttt
     2161 ctactggaat ctcattcccg ttgctatacc aattggcaaa tagcagacca aagattgctg
     2221 tttgatcagg tcctttccac cacatctctg agtattggga agaatagttt tctgcttcca
     2281 aaacttgatt atcaaagcca attggtttta caccacggcc aaacgctgct gctgaaatac
     2341 cagcttgttt cattaattga ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc
     2401 ctaacatgac aggggttccc catttacggc tttcttccat gccaatcagc atatttctca
     2461 cgttcgattc cgagctgata agaaaatcat cttgcaagat ataaaatggt ccaattctca
     2521 attttccttc attgattgct tgttggattg cctgtctttt ttctggcctt acttgaagat
     2581 agtcatctaa aataatcgtt tgcccatcca aatgaaaact gttgaaggac ggatcaactt
     2641 caaatagctc tagtagatcg tctatcaatt caatcagtcg catgtgatgc tgttcgtaag
     2701 ccatgtacca ttcacgatcc cagtggctat gagaaatgat atatactttc tttttcattt
     2761 acgatacctc ctacttttct actctaatat caaagtaatc catgactaat tcgcaaaaca
     2821 tcatatttgc ccaagaaaac cattctctcg tataattttc aggattgttc acatcgaatc
     2881 cttcgtgcat aaggtgtgtt cctgcgtcag ttgctacaag taaattcaat atccgctctt
     2941 tttcagattt gtctgacgtt gtcattcctt ccatagctaa tgcaatcggc caaacgtatt
     3001 tttctggcgt atgggaggat ccaattcctt ttgcgtactt tccttcatag aaatatggat
     3061 tttctttact caacaatgtt cttcttgttt gcagataatc ttcctcttgt tcagaacaat
     3121 atcccaagta aggtgcggcg atcagattgg gcacattact gtcatccatg actgtggcgt
     3181 ttcccattcc atctacttca tatgcataaa tcgtttgccc tttttgatta accgttcgtc
     3241 cgtgattctg aatcccctct tggatctctt tcttcaattt ctgtgtttgc tgtacaattt
     3301 ctttatcatc gagtatcgtt tgaaaaattt cttccacata acctaacacg accaccgcaa
     3361 acatattgga tggtaccaga tatccataac ggcaagtatc atcgctagga cggaaaccag
     3421 accatgtcat tcctgtaaaa gctgtttttg cacctcgccc gtcattgatc agcgtatctt
     3481 cttttcttgt cgtgtctcgt acaaatgtat aaggagagtg ttcatgatct tgctctgttt
     3541 tgaacacgtc caagattttt ttgatacctt caataaaaga ttgattgaat tgatcagttt
     3601 ttcctgtatt tttgtaaaga agataggcca actgtattgg gtagcataaa gaatcaattt
     3661 catattttct ttcccagatc caatcgttca tttttgtatg atctgtttgg tggccagcac
     3721 cgttagcttc ttcgttgaag gcattcgcat atggatcgat attgatataa aaaaattgct
     3781 ttttgactaa cccgctgatc atgtcagcta attcctcatc ttcttttgca atcactaaat
     3841 aaggacgaac ttgtgcagta gagtctctta accacatcgc tggaatgtct cccgtcaata
     3901 aaaaggtggt tccgtcattt tgctttttga ctgttgtcaa cagagtattg gcaaatgctg
     3961 ctgtaaaatt ctttgcccag tccgcatgga tatccccaca tttttgtgtg atatcttcca
     4021 taaatttctg caccgatttt ggtatatctt tataagccac taataaaacc ttctttctat
     4081 ttgatatatc tttaaagatt atacttcata cagccaatta tgtctacgct ttcaaaaaat
     4141 atctttttat ttactttttc atccattatg gtattatttg aatatgatat atcattaaga
     4201 ggtgtttata tgaatcaacc attgtaccag acaatattta atgatttgca aacggctatt
     4261 ttaactggtg aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc
     4321 tataaagtaa gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt
     4381 atttatcgcg ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt
     4441 gcttctccta caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct
     4501 gttggtgatt ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg
     4561 atgacgacgc ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac
     4621 ggattgattt attatgctct tgatacagaa caacatcttg acttactttt tgagctttca
     4681 ttgaaagaat tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc
     4741 gttctttccg ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga
     4801 cattcaaaaa tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt
     4861 taccttggtt accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat
     4921 gacaaaagcg cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct
     4981 tttgtttgtg aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt
     5041 tatcatatcc ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt
     5101 gttgacccgc cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga
     5161 gatgctttgg tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt
     5221 tcattcatca aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac
     5281 tagacatgca acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg
     5341 cgttcctttt gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg
     5401 gtttcatcct gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg
     5461 gatgggtgat ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac
     5521 ccttttccat gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct
     5581 gtcgatctgt caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg
     5641 aatcttatct attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg
     5701 ccatcagctc tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg
     5761 ttcagaagat cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat
     5821 tgatgagtta gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac
     5881 aaaaaaacaa actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa
     5941 cctggaacta gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg
     6001 gcagtcctat tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt
     6061 ttatcataat ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag
     6121 tgggcaaaaa atccactatg atacgactag tcgatctata aaacctggtg ttctctatac
     6181 aaataatggg ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt
     6241 agaaaaatac gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt
     6301 gccaaaatgg ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt
     6361 cattgcagat gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc
     6421 aatggaaaaa ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga
     6481 agattacttg acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt
     6541 agactatgct tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga
     6601 aatgacgaag aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc
     6661 aggatttatg cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg
     6721 ttggggacaa gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga
     6781 tttttcagga ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga
     6841 gctgtgcaat cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat
     6901 gagtgagatg gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca
     6961 ctatccttac ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca
     7021 gttgatgact cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg
     7081 aagtatggcc gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac
     7141 cggtgaatat gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg
     7201 aaaggaattg acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat
     7261 caaacggatg gacaaagaat atacgaaatt atattttacc catcaagatt tgctgaaagg
     7321 cggcacgatc tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga
     7381 tttgcctttt tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat
     7441 caagtaataa gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta
     7501 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg
     7561 acctgagtat tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc
     7621 taataccgag ggtcagctga ttgaacaccg tttagtggag aggtcgtgaa aaagctgttt
     7681 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
     7741 gcttaatacc
//
LOCUS       DAZKOM010000116         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000116 DAZKOM010000000
VERSION     DAZKOM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.120"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG8NF_002522"
     CDS             <1..848
                     /locus_tag="ACG8NF_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0472948.1"
                     /translation="TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEM
                     TDLWGIGSRMKKRLNQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAI
                     PPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTS
                     ILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQL
                     DLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAG
                     GLKND"
     gene            841..1191
                     /locus_tag="ACG8NF_002523"
     CDS             841..1191
                     /locus_tag="ACG8NF_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472949.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1173..1358
                     /locus_tag="ACG8NF_002524"
     CDS             1173..1358
                     /locus_tag="ACG8NF_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472950.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2262..2537)
                     /gene="prgO"
                     /locus_tag="ACG8NF_002525"
     CDS             complement(2262..2537)
                     /gene="prgO"
                     /locus_tag="ACG8NF_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0472951.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(2509..3462)
                     /locus_tag="ACG8NF_002526"
     CDS             complement(2509..3462)
                     /locus_tag="ACG8NF_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0472952.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4074..5567
                     /locus_tag="ACG8NF_002527"
     CDS             4074..5567
                     /locus_tag="ACG8NF_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0472953.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            5702..5998
                     /locus_tag="ACG8NF_002528"
     CDS             5702..5998
                     /locus_tag="ACG8NF_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0472954.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6099..6950
                     /locus_tag="ACG8NF_002529"
     CDS             6099..6950
                     /locus_tag="ACG8NF_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472955.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6971..7183
                     /locus_tag="ACG8NF_002530"
     CDS             6971..7183
                     /locus_tag="ACG8NF_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472956.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2612 a         1175 c         1419 g         2331 t
ORIGIN      
        1 ctacagtagg tgtcggagat aatcccttat tagctaaact tgctttagac aatgaagcaa
       61 aacataatga tggattcctt gctgaatgga cgtatcaaaa tgttcctgat aaagtatgga
      121 atattcctga aatgacagac ctttggggta ttggatctcg aatgaaaaaa agactcaacc
      181 aaattggtat catgagtatt aaagacttag ctaattggaa tccatacact attaagagcc
      241 atttaggtgt gattggctta cagctgtatt ttcatgcgaa cgggatcgat cgaacagata
      301 tagcgatccc accagaaccg acaaaggaaa aatcatatgg taattcacaa gtgttaccac
      361 gagattatac caggagaaat gaaatcgaac tggtggtaaa agaaatggcg gaacaagtag
      421 ctattcgtat caggcaacat aactgtaaaa caggttgtgt ccatttatac attggcacgt
      481 cgatcttaga aacgagacca ggtttttcac accagatgaa gatccctata acagataata
      541 caaaggaatt gcagaattac tgtcttttct tgtttgataa gtattatgaa ggacaagaag
      601 taaggcacgt tggaataacc tatagcaagc ttgtgtacac tgattcgctt cagctggatt
      661 tatttagtga tccacaaaag caaattgacg aagaaaattt agataaaatt attgacaaga
      721 ttcggcaaaa atatggtttt acgtcgattg tacatgcttc ttctatgtta gaaggcgctc
      781 gtagtattac tagaagtaca ttggttggtg gccatgcagg cggcgcagga gggcttaaaa
      841 atgattgatt ataatgatcg tggacgttta aagtgggcag gtttttattt atctgatcat
      901 actgaaaaaa ttgatgccga caatgaacaa cgagccaatc aaaatttggc aaaagaacaa
      961 atgacgactg aagaggttgc ggaagtattg aacgctgcag tcataaaaaa tagatcagta
     1021 tgtattcaaa aagaagaacg aaacgctgaa ggatactatc cagaagatat tgttagcaag
     1081 atcttaggta gtgatgagct ggggatctac gttggaaaaa acaaagttca ctacgacgaa
     1141 atacgtcata tcgaatttta ccaggataat aaatggggtc gattagaatg aacattatcc
     1201 agcaatatga gctaaagtat ataacctttg atcagctatc agaagagatt tggggttatg
     1261 gccagcgttt gataaatgag gttggtgttg aacgcttctc tttctatgta gaagctgcag
     1321 caggatacca taaatacgaa tactatattg gctattaaag tgcaataaaa ttatatttct
     1381 tttatgtttt acattttgta tcaattctga taaaattacc tataggtaat taaaaaatat
     1441 aaaaaaacta gttacaacct taggacgttg taactagctt gaagcatact agagctaact
     1501 aaaatagcac tagaaagcaa tgttaaaccc gtccaacaaa cttgatttaa cattgagacc
     1561 agcttattca gctaaatata gttgaataag cttcttttgt tttaaaaaaa caaaaggtgt
     1621 actacgaata acgcaagtaa aagaaatact attacaacca gtaggctaca agtaagtatt
     1681 actaaacatt gcgtagttag aacctttgct ctattgtcta aagccacaaa attagagatc
     1741 agctttatga tgctaagagc gagctgcatc catgcggaca taggttcttt tgacactatt
     1801 aattctcctt tttaggataa gggaaattgc tagtagtttt ttcattagca ccatccccct
     1861 tcatttgatt gagtctagtg ctgctgtgat agtttgctgt ttaatatgct tacattctta
     1921 ttataacata aatgaaacaa acacatggtt agaggtcgta tcaatcaaaa tagtgtctca
     1981 caaacgattt attacttact cctgctgact aacggactgt tcttcagtaa ctgtaactat
     2041 atgtttgtta aaaccaccat tacctgtaga taaaaaccaa tatatttact atactcacta
     2101 tattaaatat atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac
     2161 ccccaaacaa tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt
     2221 atactaaata tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta
     2281 ttatatataa tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg
     2341 actatttcat acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg
     2401 ggaggatcta cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg
     2461 acattaatat catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc
     2521 gttggtaata atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat
     2581 tgagtcagca tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta
     2641 ttcaattctt tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc
     2701 tacataatca tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt
     2761 aacaatgctt ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc
     2821 gtgttgagag gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg
     2881 agactcttca aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc
     2941 ttccattgcg taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa
     3001 tgcacgaacc acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt
     3061 tctcaattct tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt
     3121 ttcggacaag tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg
     3181 tggacttact tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga
     3241 tcgatcaaag ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg
     3301 gtcataatca attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa
     3361 atcattgtct gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc
     3421 tagtttatct gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct
     3481 atactaaata tatttagtat acttcatata cttaatatat tcgatatatc taatatactc
     3541 caaattgata agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct
     3601 atattcagta tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac
     3661 aatttagagt gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta
     3721 caaagtagca aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg
     3781 aaataattga ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca
     3841 cgctcgtctc aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata
     3901 ccttttccga ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaacgttt
     3961 atttagtgta cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg
     4021 agattaaagc taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga
     4081 gcgaaattta ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca
     4141 ccatgaaacc tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta
     4201 gcaagcaatt aatgaatgtt gggcgtggta tggtcattac ttcggaagaa gctattttag
     4261 agaacgaaaa gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg
     4321 atgattatcg aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat
     4381 ttgtagttga tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag
     4441 atcaagtggg ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt
     4501 ttgtactaaa gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag
     4561 caaaaaaaat ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc
     4621 taggatgtaa ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag
     4681 aaatgaacta tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag
     4741 atcaaaaaac aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa
     4801 ttcgtcaagt agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg
     4861 gagaaggacg tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt
     4921 caggttatgc attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg
     4981 aaccgctatc tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc
     5041 aagcagcaag cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa
     5101 aaaataatga tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc
     5161 aaagaaaata tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa
     5221 aaagtcttgc tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa
     5281 cgatgcccaa gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct
     5341 tctacaaggt aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt
     5401 tcgcgagggt tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag
     5461 aggtatttgg actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct
     5521 tgaaccctct taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg
     5581 caaattgccc tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc
     5641 tacgtgtagg tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg
     5701 tatgtcgtta aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt
     5761 gggattatct gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc
     5821 ttgggttacc agggaaaaaa cagttgcttc tttgccgatt gagtttcttc atgggttgtc
     5881 cttagcttct ggggagacta tggatcaggt atatgattgt ttgtgtgtgt tagagcagga
     5941 gtatattgag tataaaatag ctaacgagtt aagaaaaagg aagaagtata tccagtagta
     6001 gtttttattt acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag
     6061 gaaaacgtta aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg
     6121 aacacacctg gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat
     6181 tatggccatt aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt
     6241 actcgattgg agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag
     6301 catcagacat ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta
     6361 ctcaattagt catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc
     6421 tattcgattt aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat
     6481 ctggaagaac aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg
     6541 gacacatgta tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga
     6601 aacttattaa acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg
     6661 gttatgagta caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc
     6721 acacgagtat ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg
     6781 aaatgaaagc aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc
     6841 tacgatatgg catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat
     6901 acattttttg gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa
     6961 tcgtttgaaa atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac
     7021 cttaaagaag gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa
     7081 aacgggtacg atgaacattg aataccgaac agaatattta gaattaacga atcaaactga
     7141 ttttttgttg attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa
     7201 ttgattgatg aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct
     7261 ttatttgtaa agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg
     7321 ctgtgcctat tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag
     7381 tgaaaggata gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc
     7441 ttttcgctat cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga
     7501 tgagaaaatc gtaagagaaa ttgatcgaag atgtgac
//
LOCUS       DAZKOM010000117         7425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000117 DAZKOM010000000
VERSION     DAZKOM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.121"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACG8NF_002531"
     CDS             complement(<1..252)
                     /locus_tag="ACG8NF_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472957.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLT"
     gene            complement(370..630)
                     /locus_tag="ACG8NF_002532"
     CDS             complement(370..630)
                     /locus_tag="ACG8NF_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472958.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(767..1180)
                     /locus_tag="ACG8NF_002533"
     CDS             complement(767..1180)
                     /locus_tag="ACG8NF_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0472959.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1636..2121)
                     /locus_tag="ACG8NF_002534"
     CDS             complement(1636..2121)
                     /locus_tag="ACG8NF_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472960.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2260..2475)
                     /locus_tag="ACG8NF_002535"
     CDS             complement(2260..2475)
                     /locus_tag="ACG8NF_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0472961.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2699..3499
                     /locus_tag="ACG8NF_002536"
     CDS             2699..3499
                     /locus_tag="ACG8NF_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0472962.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3518..5386
                     /locus_tag="ACG8NF_002537"
     CDS             3518..5386
                     /locus_tag="ACG8NF_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0472963.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5379..5870
                     /locus_tag="ACG8NF_002538"
     CDS             5379..5870
                     /locus_tag="ACG8NF_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0472964.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5858..6412
                     /locus_tag="ACG8NF_002539"
     CDS             5858..6412
                     /locus_tag="ACG8NF_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0472965.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6430..7425
                     /locus_tag="ACG8NF_002540"
     CDS             6430..7425
                     /locus_tag="ACG8NF_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0472966.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2634 a         1194 c         1419 g         2178 t
ORIGIN      
        1 cgtaagagag atcataagcc ccaatgaatg actaaaaaga aaagcagaat cacatgccat
       61 atcatcccat atttcttcaa gaaaataaat aagcgggaaa aagggaagga atagtagaac
      121 atcgatccag caccattttt ttcttctttt gaaataacat aaaacaataa acatgaacgt
      181 gcagggaagt aaaaacaaag ctttactcat aactaacaaa ataccagctc cttttatttt
      241 tttctccccc acattcaaag agtaacaatg gtatgtactt aataatagaa aaatggatca
      301 acttgtatga ataacagctg aaaagatctg caccaaataa aaaacttcca gccaaaactg
      361 actggaagtt tatttttttc tgaaatattc taacacaaat atgaaaaaga tggccgaagc
      421 taataagccg atttcgtagc aaaacaatga gataccagag tcgtaataat aaaatcctct
      481 aatcttatgg ataaaataga tcatcggaca agcagataag agtaagtatt tgttaggcct
      541 tttgaataag taaaagaaag cactggtcat tatgataaat ggtatccaaa acaaaagttt
      601 acttaagaca atcaaatgat caactcccat attttttacc aagagagatc ataacatctt
      661 tatcgtaaaa acaaaatatt tttttgctca ttttgaaaat ttaaacatac tcgttcttat
      721 ccaaatatat aaaaatcgcc cctcattcat cgagaggcga taagttctaa tttaactcaa
      781 ttttttttgt ttcttttggc tgataagctg ttttaggtaa taccaatttc agcacaccgt
      841 ttttaaaggt tccagtgatt ttttcttcat caacattagg aagatagaat gaacgtttgt
      901 aagagaccga atgtctttct ttccgtacat agttgccatc ttctttatct tctgtatggg
      961 actcatgatt ggcggagatc gtcaaagtat tatctgaata ttcaactgtc gtgtcttctt
     1021 tgtcacagcc aggcatatca gcagttagtt tgtattcttt ttcattttcg actaaatcta
     1081 cttttggata acttgaaaca cctaaaaaat cattgaatgc tggactaaac acgtcattga
     1141 aatcagggaa catgtcgcga atgctaggta catttgccat ttttgatcat cctttcaata
     1201 agtatttttt atttatccct atttacgtaa taactctatt ttgaaaaaaa tgcaagtata
     1261 atggttagct ttgtgaatat ttcatctaat acgatacaaa ggctatcttt ctaatttgaa
     1321 aaagggatca ttaaaataat aagaagaaaa tatgtcaaaa ggtaaaaaag taaattcttc
     1381 tatccaaata tagaaaaatt gaatctagaa aaccgcccga caataaattt cattaactca
     1441 gttgaccgaa aatgaagtag ataccaaaaa tttatcaaga gagcaagtag agaagtgagt
     1501 atattcataa gagaaatagc tgtaacgaat tattaggtta atgcaaatgc ttatttatac
     1561 tccgtataac tttctcagaa aaaatgatac caataatgac ttgctagtac tgtaaatccg
     1621 tataatatca tctaatcaaa atggtgttcc atttgcatct gtcatcgtaa tcgatccttc
     1681 gggtgacact cgataaaaac caacgcttcc agatcctcct tgggaacgga tttgtttgct
     1741 ggaagctgtg aacaaataat ctcgatcaat tttttgataa aagctaataa caatatcgtc
     1801 actttgtaat tcggaacggt gggcaataag aaatagaagt gctttgtcag agttttctaa
     1861 tggaagagaa ggtacagtgc tttcttgcat ttcagatgtt gaagaaatgc taaacgactg
     1921 aatacttgat tggctatgtt gcgaatctac attaccagta gaagtattag taatggaagt
     1981 agtagaagta cttttagttg aaccaacctc agttgttgaa atggctgctg aagattcttt
     2041 aacagaattc tttgttttat atccacaggc agaaaaaagt attacagttc ccaacaagcc
     2101 tgcgtaaatc cctttatgca tgatagctcc tcctaaaata gattatcttt attcagtata
     2161 tcacttaaac aaagaaaagc agatggttta aaatgaaaaa accttcccaa taaagggaag
     2221 gttcatgaag ataataactt ttttaataat tatagctttc taatatcttt catcaacttt
     2281 tctagtaatc attgggaaaa atttcaagca gtagatcgca caaataccta cgacgatata
     2341 aatgatcttc gcaaaaatag ttgttgaacc accagcaatc atagctacta aatcaagttc
     2401 gaacaagcct accaacagcc agttcaaccc tcccacaatc aataaagtta atgcaatagc
     2461 atctaaagtt ttcattagaa ttctcctttc ttaaatgata aaacaattat atatcaaaaa
     2521 gagaaacatt gcttatataa cgctcaggaa ttttagcaat ctatgcaaaa agatgaaaat
     2581 tttttttgct cttccattag tacacatttt ttagctgaag ctgtaaattt catgtcttaa
     2641 ttttttttag tgagctttta tactggattt gtaagaaaaa agaaggagag gatcacatat
     2701 gtcggattgg ttggatattg caggaaaagt agtaattgta actggaggtt cctcaggaat
     2761 tggaagaagt atcgtcgaaa atttgttgaa gcagaatgca caagtagcga attttgatgt
     2821 aactgaatgc cgtatacagc atgaaaacct actatcttta aaagtcgatg tgtcttctaa
     2881 aacggacata gaagaaggga tatataaagt aatgaaacat ttcaagacga tcgatggtct
     2941 agtcaataat gcaggaataa atattccttc tcttctaata gatagaaatc atccaaaaag
     3001 caagtatgaa ttatcagaac aagtttttga taagatgatc gctgtcaatc aaaaaagtgt
     3061 atatttgatg agccaagcag ttggaagaat cttggtgcaa aagggatcag gagtgatcgt
     3121 gaatctttct tctgaatcag gattggaagg atcagaagga cagagctgtt atgctgctac
     3181 aaaagcagcg atgaacagtt ttactcgttc ttgggcaaaa gagttaggaa agaggaatgt
     3241 tcgtgtggta ggtgttgcac cagggatact ggaagaaacc ggtttgagaa cacaagaata
     3301 tgaagaagct ctttcttata cgagaggtat aagcgtcgaa cagctgagaa acggttacag
     3361 tcgaacaagt acgatccctc taggaaggag tggtaagctg caagaagtgg cggatctagt
     3421 ctgctattat ctttcagaaa gatcaagtta tatcactgga gtcacgacta atatttcagg
     3481 aggtaaaaca agaggataaa ggataggggg gacgcacgtg acgctagtaa acagatggta
     3541 ccaattattg aaattactag tcactcacaa gaaagtttca atccaggaag tgcaaaaaga
     3601 aatcaaaacc agtaatcaaa ccataaaaaa agatattggg gagttgaatg aagaaattgc
     3661 cggtattgca aaaatcatcc aaaaaaacaa acattttgaa cttgaaattt atgattttga
     3721 tttttttgat gagatcatgc aaggaaaatt gaaaacagcc agtgatttca attcggcatc
     3781 caaaagaatc gcttatttac taaataggct gatcgaaaca gaggatttct tacgtatgga
     3841 tgatttgtct gaagaagtcg gtgtcagcag aggaacagtg gtcaaggatc taaagacatt
     3901 aaaagagatg atcaatgatt ttgatgtaaa gattacaggc acaccaaata aaggactaag
     3961 aataatagga gatgagctag aattacgact acttttattt tattttgttt ctccctactt
     4021 ttcagagacg aaaacagaac agtttctaga agaaaaacaa gcaatcaaac attttcagaa
     4081 gaagacgaat gctagtaaac aagaaatcac ccttttaacg aaagttatcg gtatttcttt
     4141 gaatagaatc tctagtaact atctgttgaa gaagcccatc aatcgttaca gtgggtgttt
     4201 tgaatatatc caagaattca atgaactgat caatcagttg gaagaaatct atgaactaac
     4261 tttgagccaa tatgaaaaag attttatcag ttttcctttg aacatcatca acacaagtat
     4321 aacgatgata aggaaagcta actctgattt gcgcttatat ttcgacccca tgatgatgcg
     4381 tattcgacga ttagatctca ttgatctgga tgagtccttc ttatatcaag agatgaaaca
     4441 gcacctctct ttattgataa atcgggtaat ttttcgttat cgattgactg atttatttta
     4501 tggtgagata gaaaaaaagt atcctttttc ttaccaaatc gctagcgaat gcatacaagc
     4561 attagaagct atcttgcaac ataagatctc taaagtagag atcagctacc taacgttgta
     4621 tttggagtta gcatttcgat atcaaaaaga gcatttttcc tctaaaaaag tagccattgt
     4681 tcatacgaca ggaaaaggaa cagcattaat gatccaaaga caaatcaaaa ggatattggg
     4741 gagggatata caaataacac atgtttcaga agaacaatat gaaaaagtag atctaaacaa
     4801 atatttcgct gtttttacaa cgattccgtt aaaaaatgta aatgaagaga caccggtgat
     4861 ccatttgact aatctttttg atgactcttg gttaagggca caatggcaaa aagcaaaaaa
     4921 aaagcgatta gccgggtttc attctactgt ttttttctat caaatatgga aagaaaaaaa
     4981 gacgtatcaa gaatatttaa acaaattagt cgctcctttg atcagaggct attatgtgga
     5041 tgaagaattt gaaaaaagga tcttgaaaag agaatatcag caaagcacat ttgttaacaa
     5101 agaatttgct tttcctcaca caaccaacca gctatctcaa cacattgttt tatcagtagg
     5161 gctaataact gatcaaggtt tagcagaaac aggaggaaat ggagttattt ttttatttgc
     5221 catacctgaa gaaatgacgg ctgaagttga gaatgaatta tttgagctat acgatatcct
     5281 atttgaactc ataaaaatgg gaatagatga gtttattgaa ttagcaccga cacaagaatc
     5341 atttttaaaa ctaatcaaaa gtaaaggaga gagacttcat gaatgatatg tatctattca
     5401 tagttttcgc agcttcagct tatgtgctgc aagcagttat cagttggaag caactaaagc
     5461 atttcaatca aacatatagc gaactgcgaa gaaaaggaaa agtagcgatt ggtaaacgac
     5521 ctgggaaaat ccgttcaggc acgattgtta tgctagctat agacgatcat gatcaagttt
     5581 tagaagtcag aaaaatgcaa ggggttacat ctttggcaaa atttcattca ttacctgtct
     5641 atgcgggtga agatatccat tacatcgatc gttaccatcc ctcagttcga gcggaagatc
     5701 gtttgacaca agcagcttta gaaaatgcca gagaagtata cttacgaata gaggcaggaa
     5761 atgaaaaaag tgaacaacct tcattgcctt ttattgctat gctcaataat tttcggatat
     5821 ggaaaagtac gatcaaagaa aaaaggagtg acaaacgatg gatatcataa caaaatttgc
     5881 tgagggattt atgaggctgt tccaaacagg tgcagaaaca ttcataagct ggatgacagg
     5941 aatcgtacca gttgtcttga tgctgatggt tgcaatgaat acgctgattg cttttttagg
     6001 tgaagaccga gtaacgaaag tagccaaatt atcagcgaaa aatccgctta ctcgttacct
     6061 tgtgttaccg ttcttatctg cttttatgtt gggaaatcct atgtcattta caatggctcg
     6121 tttccttcca gaatactaca aaccaagcta ttatgctgct caagctcaat tttgtcatac
     6181 gagcaatggt gtattccccc atattaatcc tggagaattg tttgtatgga tggggattgc
     6241 tcaagggatc gaaaccttag ggctaaattc gatggagctg gctatccgat acttgcttgt
     6301 gggactgctg atgaacttta ttggtggttg gataactgat tttactactg gatttgtatg
     6361 cagacagcaa ggaatcgttc ttagcaaaaa agtagagctt tcagttgatt agagaaagga
     6421 gagttttaaa tgtatggtga tattaagatc gtaagaggaa acggcgggta tggaggtcct
     6481 ttgattatta ggccgacaaa agataaacac aagttcatct atgtcacagg tggaggtgag
     6541 aggccaatga tcgtagataa aatagaagaa ctgacaggta tggagtctat caatgggttc
     6601 aaaacatcaa tacctgatag tgaaatcgcg cttgcaatcg ttgactgtgg agggactctt
     6661 agatgtggaa tttatcctaa aaaagggata ccaaccatca atatcgtttc cacaggaaaa
     6721 agtggcccat tagcacaata tatcactgaa gatatttatg tttcagcagt agggttagaa
     6781 caaatacagc cactaatgga aactgatgat aacaaagaaa cagtaaatac tgcatctcca
     6841 gataaaaagt acaaatatga tacttctaaa aagataaccg agcaaaaagc agagcagcaa
     6901 aactggatag caaaaattgg aatgggcgcg ggaaaagtag ttgctacatt caatcaatca
     6961 gcaagagaag cagtagatac gatgatccgg acagtgatcc cttttatggc tttcgtttcc
     7021 cttttgattg gtgtgataca aggttctggg atcggtaatg tatttgctaa cattatggca
     7081 cctttagcag gaaatgcagt tggattaaca atcattggct ttatttgttc actgccattt
     7141 ttatctcctt tgcttggtcc aggggcagtt attagccaag tgattggaac attgatcggt
     7201 gtagagattg ggaaaggaaa tattcctccc caattagcat taccggcact atttgctatc
     7261 aatactcaaa atgcatgtga ttttattcca gtcggtctag gtttggaaga agcaaaagct
     7321 gagacagtag aagtaggtgt accatcagtt ctttattcaa gatttcttaa cggcatccca
     7381 cgagttatca ttgcttgggt agcaagtttt ggattatata gctga
//
LOCUS       DAZKOM010000118         7423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000118 DAZKOM010000000
VERSION     DAZKOM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.122"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(456..881)
                     /locus_tag="ACG8NF_002541"
     CDS             complement(456..881)
                     /locus_tag="ACG8NF_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472967.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="ACG8NF_002542"
     CDS             1255..2226
                     /locus_tag="ACG8NF_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0472968.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="ACG8NF_002543"
     CDS             2258..3655
                     /locus_tag="ACG8NF_002543"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0472969.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3814..4437
                     /locus_tag="ACG8NF_002544"
     CDS             3814..4437
                     /locus_tag="ACG8NF_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472970.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5186..7279
                     /locus_tag="ACG8NF_002545"
     CDS             5186..7279
                     /locus_tag="ACG8NF_002545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0472971.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         2520 a         1234 c         1498 g         2171 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctatt tcattttttg tatataatag
     3781 aatagtcaaa attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc
     3841 ttttggttat tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg
     3901 acagcagatc ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat
     3961 aatttcaaaa aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac
     4021 gaaaaaggcg aattggacat ttatttgcca aaaggaaaag aagaaaatca atttattaat
     4081 atcgatgacg tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg
     4141 accgttttgc ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg
     4201 atcaccgctt attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg
     4261 aaaaagacaa atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca
     4321 ggaacaagtc cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa
     4381 gcctcaatta ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa
     4441 acagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gcaaaatagt
     4501 tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgtgta
     4561 tcaagaggtc ttgagcctgc cgtttaactc tgagccacaa ggagcaattt caaaaaatag
     4621 ctccttatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     4681 ttaccaagag gttgtaaaat tagcgtcctg acataatcta ataccgaggg tcagctgatt
     4741 gaacaccgtg tatcaagagg tcttgagcct gccgtttaac tctgagccac aaggaacaat
     4801 ttcaaaaaac agcttctcat gttttataaa tattgtgact tgtggccgag gagttggcag
     4861 gagaacaccg tttaaggaag aagttgtgaa aaagctgtcc tgcatcaaga aataaggagc
     4921 tgcgacaaga attttgtcac agcctctttt tttgtacgaa taccgagaaa ctggcttttg
     4981 aataccgtgg agtgaagcgg tcaccacaag acttttatct cttcattagt ttaatcgctt
     5041 acatcgcagc ggttttatgc tgtgaaaagt ggacggaata atctttagaa agaataaaaa
     5101 aatcttaaga taacaacttt tttaggaaat tttcattttt caggagcaaa ataagaaaaa
     5161 atgtgaatag agagaagtga tatacgtgac acaacttaaa aaattgtgcc aaaatcgttt
     5221 agcaatcgtt ttgacagctg ttttcttttt ctggctgaag acgatcattg cctactatgc
     5281 agatttttca ttaggtgtgg aaggtacgat ccagtacttt atcttgtgga tcaacccaat
     5341 tgcgactacc ttattgtttt ttggcctttc tttgtatatt aaaaagccaa agccagcgtt
     5401 agctattctt ttgatcattg atattttaaa tacgcttttg ttgtacttga atattatttt
     5461 ttatcgggaa ttcacagatt tcattacagt caaatctgta ttaggctttt cgaaagtgtc
     5521 gcaaggattg tcaggaagct ccttttcttt gatgaagccc catgatgtca tctactggct
     5581 ggatatcgct gtgttcattg ggctactggt ttggttgaag gtcaagaaaa tacctatcaa
     5641 aagcaaccct gtcggcaaac cgatggctat cgctgttacc tgtctttcag ggcttatttt
     5701 ttcaggaaat ctagcgctga gcgaagccaa ccgtccacaa ttgctgcagc gaacatttga
     5761 ccgttcatat attgtcaaat atctaggaat cgatgcgtat actatttatg acggaatcaa
     5821 aacaggaatg actagcagtg ttcgcgcgca tgcaagcagt aatgggattg atgaagtttt
     5881 agattataca aagaaacatt atgcagagcc aaatcccgaa acatttggga tcgctaaagg
     5941 gaaaaatgtg atcgttctcc atttagaaag tttccagcag tttttgatta atatgaaggt
     6001 agacggacaa gaagtcacac cgtttttaaa tagtattttc cagaatcaag caacgatcag
     6061 ctttgacaat ttctttcatg aagtaggaca gggaaagaca agtgatgctg aaaatatgtt
     6121 ggaaactggc acatttggtt tacctcaagg ctcattattc acagaacttg ggtcagacaa
     6181 tgtctttcaa gccgcaccag cgatccttgg acaaaagcaa ggctatacaa gcgctgtttt
     6241 ccatgggaat gtagctagtt tttggaatag agatcatgta tataaaaact taggttatga
     6301 caatttcttt gatcgatctt attttgatga atccgacgaa acgttaggtt atgggatatt
     6361 agataaagat ttattcagag aatctgccca atatcttgag catctacaac agccgtttta
     6421 cacgaaattt ctttccgtaa ccaaccacac gccttattat acggatgaca agaatttcga
     6481 ctttccatct ttgaatactg gcaatagcac agtagataat tatgttcgaa cggcacatta
     6541 tctggatcaa tcattggaac aattttttac gtatttgaaa aaatctggta tttaccaaaa
     6601 ttcaatcttt gtgatttacg gggatcattt tgggatctcg aatacagata ataaagattt
     6661 agcaagcgct cttggtaaag atccagatac atgggatgaa tttgacaacg cacagatgca
     6721 acgagtgcca ttgatgatcc atatgcctgg ttatactaaa gggacagtca atcatgaata
     6781 cggtggagaa atcgatgttt tgccgacatt actacatcta ttgggaatcg atgataaaga
     6841 ttatcttcat tttggtacag acttattatc ttctcagcat gatcaagtag ttgcttttcg
     6901 aaatgggaac tttgtgacac ccaaatacac cgtcctagga ggaaaagctt acaataatca
     6961 gacaggagaa atcatcgata cgaaagcagc aggaatcgat gaggaaatct ccaaagatca
     7021 ggaaaaagtc aagactgcgt taagtctttc agataagctg aatcaggaaa atctcttgcg
     7081 gttttatgtg ccaaacggat ttgagacgat tgatccgaaa aaatatgact ataaaaatga
     7141 agcggaacgt aatgaagcaa tcgaaaagca aaaaggcgaa cattcgacaa gtgtcttttc
     7201 aaaaaatggt aataaaacaa caaccaactt atatccagta acaagaacag acgttgaaca
     7261 gcaaaaaaat aatcaataaa caatcaaacc aaccttcagt agttagacaa cattctaatg
     7321 cttgaaggtt ggtatttttt atgaaaaaaa cattttttta tttttttcgt gtatttttcc
     7381 tttttaacgt atctatttat tgaatgtgga aaaatttaaa tta
//
LOCUS       DAZKOM010000119         7248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000119 DAZKOM010000000
VERSION     DAZKOM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.123"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..581
                     /locus_tag="ACG8NF_002546"
     CDS             <1..581
                     /locus_tag="ACG8NF_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0472972.1"
                     /translation="MSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYN
                     EPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQ
                     LIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMT
                     AAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            593..1513
                     /locus_tag="ACG8NF_002547"
     CDS             593..1513
                     /locus_tag="ACG8NF_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0472973.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1554..3011
                     /locus_tag="ACG8NF_002548"
     CDS             1554..3011
                     /locus_tag="ACG8NF_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0472974.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3356..3970
                     /locus_tag="ACG8NF_002549"
     CDS             3356..3970
                     /locus_tag="ACG8NF_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0472975.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3963..5687
                     /locus_tag="ACG8NF_002550"
     CDS             3963..5687
                     /locus_tag="ACG8NF_002550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0472976.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5684..7135
                     /locus_tag="ACG8NF_002551"
     CDS             5684..7135
                     /locus_tag="ACG8NF_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0472977.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2477 a         1207 c         1450 g         2114 t
ORIGIN      
        1 cgatgtcctt actaccttat tttctatctt gggtcattat cagttacttt gtttacgctt
       61 ttttaagtcc tgacaaagga atattcaatc agtggatcac tggacatgga ggagaagctg
      121 tcaattggta taatgaaccg aaatattggc cgtttatctt ggtgttcatt ggtacatgga
      181 aagggattgg ttataacagc attatttact ttgcatctgt tatgggaatc gatccgactt
      241 attatgaagc tgctatggtt gatggagcta gcaaatggca gcagatcaaa catgtgacga
      301 taccacaact tattccacta atgacgattt taacgatttt agccgtagga aacattttcc
      361 gagctgattt tggcctgttt tataacattc ctaggaattc cggtgcatta tatgatgtga
      421 ctcaagtttt ggatacgtat atctacaatg gcctgacatc cacaggagat tttggtatga
      481 cggctgctgc aggactttac caatcagttg tcggctttgt cttgttgatg atcacaaata
      541 cgatcgcccg acgttttgat agcgattctg cattattcta aggaggaaaa aaatgaaaaa
      601 aaagaaagtg gagagagtcc aagtccgctc tttcaataag acggctaatt ttttctctaa
      661 tataatgatt gcactctttg ctatttcctg tattttgcca tttatatttg ttatcgccat
      721 ctcttttacc gatgagtcca tattgtcgac tcacgggtac agtttttggc cagaaaagtt
      781 cagtatattt ggttatacgt acttgttcga ccagatgcag gataaaattt tccaagcgct
      841 tttcgtcact gtacttgtta ctgttttagg tacactgatc aatagcacgg ctacttcttt
      901 atatgcttat gcgatttcac ggtcaaactt tccattccgt cgttttttta ccgttttttg
      961 tttgattacg atgttgttct caccagggat ggtggccaat tatcttgtta tgacaaatct
     1021 tcttcagtta aaagatacga tttgggcatt gatcttgcca atggctgtca gtcccttcaa
     1081 tattattgtg atgcgcacat tctttaaacg ttcggtctct gattcgatta ttgaatcggc
     1141 acggatcgat ggagctagcg aactgcgaat atttgttcag atcgttttac cgttagctgt
     1201 tccaggtatt gcgacaatca gtttatttgc cgcattgggt tactggaatg actggttcaa
     1261 tgctttacta tacatccaag atgataagct agttccacta cagtatcttt tgatgaaaat
     1321 ccaaagtaat attcaatatc tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg
     1381 attagcctca gttccaggag aatctgccag aatggcgatc gtagtcatct cgacgttgcc
     1441 tattgcgatc agctatccat ttttccagaa gcattttgtc aaaggtctaa caatcggcgg
     1501 agtaaaagaa taaagaaaca aaaatataaa tcataaaatt aggaggaaga aaaatgaagt
     1561 tatggaagaa actagccttt acaggtgttt cagcgttgtt gttgggaaca ttagcagcat
     1621 gcggaggttc tggttctaaa gaccaggcag aagctagcaa ttcggacact ttgcagatgt
     1681 atcaaatcgg agataaacca gataatttcg atcaattgat ggaagtagca aacaaacgaa
     1741 tcgaaaaaga gatcggtgta aaagtcaata tcaattacat tggctggggc gattacgaaa
     1801 agaaaatgaa cgtcatcatt tcttccgggg aaaattatga tatcgccttt gcgaacaact
     1861 atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg cctaagtacg
     1921 ctgaaaaagc ttataatgat ttagatgaag catatattaa aggaaatctg gtcaatggaa
     1981 aattatatgc tttcccagtc aatggaaatg tctttgctca acaagtactt acttttaaca
     2041 aagctttact agataaatac gatctttcaa ttgatggaat ccaatcttac gctgatgcag
     2101 aaaaagtctt gaaggaattc catgaaaaag aaccaaacac ggcagctttt gccattggac
     2161 aaggcttcaa agtccaaggg aactttgatt atcctttagg gaatacgtta ccatttgcta
     2221 ttgatctgga tggagatgat tcgaaaatca tcaaccaata cgataatgaa acgtatatcg
     2281 gactgttacg tacaatgcat agctggtaca aacaaggact cattccaagt gacgctgcaa
     2341 cgagcaataa ggattatcca ctagaaggaa atacttggct aatgcgtgga gaaacacaag
     2401 gaccgtatga ttatggcgat acaattttga caaatgcagc aaaacaagaa cttgtctcaa
     2461 aagcaattac tgttccacta aaatctacag ctcaagcaca aatggcaaat tttgtcgtat
     2521 caaatacttc aaaaaataaa gaaaaatcag tagaacttct agggctgctt aacagtgatc
     2581 cagaattatt gaatgggctt gtttggggga tcgagggaga agcatgggaa aaagtcgatg
     2641 gttcagatga taaaatcaaa ttgctagatg gttatcagcc aaatacacat atgtctgctt
     2701 ggaatacagg aaacaacaag attctatata cacaagagtc tattacggat gacatgattg
     2761 ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc gattttagga ttcagcttca
     2821 acactgacag tgtcaaaaca gaattaagta atatttctaa tgtcatgaat caatatttag
     2881 atggattgaa tacagggact gttgatccag atgaaacatt accgaaattg aaagacgctt
     2941 tggacaaggc tggctatgat aaggtattga aagaaatgca aaaacaatat gatgagttcc
     3001 gacaagaata ataatgaata aagacaaagg ggttgtgaca aaagacgtgt cacaacctct
     3061 tgttccgaat aaacggtgtt caaaagccag tttctcggta ttaagctaaa tatatgtaaa
     3121 aatgtggaaa gccatattca caaaattttt cttactactc gaaactaagc ggctatttca
     3181 caaactcttg gtaaacggtg ttcaatcagc taaccctcgg tattcgctca caaaacgaca
     3241 aaatatgagg aactattttt ccgtttgttt ctctaataat caggtcaagg tgctgattgc
     3301 ccaacctttt tttgtataat gagaaagtat aaacttaatg tttggaggag aagatatgtt
     3361 agggaaggtt ttggaagagt tatttatccg gatctgggtc attataaaac taaccttgta
     3421 tttctggata tatacatttg ccggcgggat catctttgga ttaggtgcgg catggaaaac
     3481 agtcaatgaa ctattttatt tgtacggatt cgagtataaa gaaatcacaa taaaaagagg
     3541 ctggaatata tataaaagaa attttttaag aggcaatctg ctatttagtt tatttttatc
     3601 tggtactgct ttattgagtt acaatttata tttatctgtt caaatcaaag ggctcatttt
     3661 tttagctatt gattttattt tactttttgc cttgctctgc ctatttgcta cttatcaata
     3721 cagtatgatt ttagatagtg aatatacgat ctctgttcct aatctgctaa agctaagttt
     3781 tatttcggtt ttttccagct tttcgtcttt tctaaaaata attattggta gcggtataat
     3841 tttaggcgtg acttggcaat tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac
     3901 ggtttggaat ggtacgatga caacacattg gcgtgaagaa ttagataaac agttggaaag
     3961 ttatgaataa gtttcggatt ctattttcaa aaggaacctt actaaatcgt ctgttgcgga
     4021 aatacgcggt tatcatgatc ggtgtaacga tgactgctac cgttattttt agtatccata
     4081 cttgggaaca aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt caaagcacaa
     4141 gcagaatgct gaatgataaa acaaccttga gtaggatcat caaaaaccag cttgtaggga
     4201 attccgaaaa gatcgaaaat gtgacgacct acttaacaaa acccatagat cagtatctga
     4261 tgtatgtata cgaacagcaa aacagtaccg atgaattagt ttctttccca aatcaaatca
     4321 aagatctcta cgcaaattat gaagaactaa gtgcgatata tatcgtgttg aatcaactac
     4381 cggagtatta tgaatcttct agagaaaaca aaggtgggga aataaaaaag gggacaccaa
     4441 aattactaga cgccttttat atcaagttgc ctatcacaca aggaggagct gagtcaggaa
     4501 gtatttttat tggttttcaa aagaaagaac tagatatgat tttagaaaat ctaacgtcat
     4561 tcaatggact ctctttatac atgatttctg gaacgacaaa tcgactatac acattccatg
     4621 ataataaaat agctaaagaa acgtttgaaa gacaagaaat gatcatcaca gagagtctga
     4681 agaaaaattc acaattgccg attgacgatc tggaaaaaaa caattttgtt caacaccaag
     4741 aactgtctga tgattatcgt atattagctg tactagataa aaaaagtgta aaaatcgaaa
     4801 cgggaaaaaa tcttgccccg ctgatcattg gtatcattat tttagacaat atactgctta
     4861 tctttcttta taaaacattc aaacgatatt cacagcaagt atctatcgtc atgcaggcca
     4921 tggatcaaac agcagcaggg aatttagaga cgcggatcga cataacaaac acggaatatg
     4981 aactgaagga actttcaatc ggtatcaacg aaatgttgga tagcatcaat cagtttgtag
     5041 aagacatcta caaattagaa atcaaacagc aagacgcaca tatgcgggcg cttcaagcac
     5101 agatcaatcc acacttttta tataatacat tagaatatat ccgaatgtat gcaatcagcg
     5161 aaggaagtga agaattagca gatgttgtgt atgcgttttc cgctttgctt cgaaacaata
     5221 cgaatcaaga gaaaacgatc actttaaaag aagaactgga cttttgcgaa aaatatgtct
     5281 atttgtatca aatgcgttat cctaatcgag tagcctatca ttttatgatc gatcctgatt
     5341 tggaaaagat cgaagtacct aaatttgtga tccagccatt agttgaaaat tattttaaac
     5401 acggtattga ttttactcga ttcgataatg cgctaagtgt caaagtcttg caggaaggca
     5461 aacgggtaag gatcatcatc aaagataatg gaaaagggat gactgaaaaa cggctaaaac
     5521 agattgaaga aaaactgtct catcctaaag tggaacttca cggttctatc ggtctgcaaa
     5581 acgtgaatga acgtttacgt gccagcttcg gcagcagtta ttatatgtcg cttgagaata
     5641 atgagacagg tggactgacc gtttctatta cattcaagga gggatgagcg tgtatcaagt
     5701 gttattggta gatgatgaat acatgatttt gaatgggtta aaaaagataa ttgattggca
     5761 aagtttaggc tttcaaatag tagcaacagc agagaatgcc aaagaaggac tagctgtact
     5821 tgaacagcga cagattgatt tagtagtaac ggatgtgaca atgccagaaa taaatggact
     5881 ggaatttatt gaagcggctc aaaaagaacg tcataatttt gaatttatga ttttatcagg
     5941 ataccaagaa ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga attatttgat
     6001 gaaacccgtg aataagttcg aattggttga aagcctcaag aaaatcaaaa cacggttaga
     6061 tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa gaactgttat tcagtcaatg
     6121 gttgaatgaa gaattggatg aaataagtga agaagaaata ataggacaaa taagtgagaa
     6181 gcagcatagg atagtattga ttcaactggc aagaactcat gatactctta tcaatagatg
     6241 gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat tacggagacc gtgtattgat
     6301 cacgttgttg ttggaggcag atgaagttga tggcttctgt tgttttgttc aagaaaactt
     6361 tcctgatcag gaatggctga tcagtatcgg ggaagagaca gattctttgg acaaaatccc
     6421 tgaaacattc caacaagcga aagacaatct acagttgcat caattttatg gagataaaga
     6481 cgagcatgtt ttttacgcag tacaagcaga aacaaaagat caaaccatcg atttttcaag
     6541 tttcagacgc gtgctgcaaa ataaacaatt agaagtcgct caaaaaatga tcactgattt
     6601 ttttgaacag tttcagttag cagccatgat gccggaagat atacgatatt ccagtttttt
     6661 actgtttatg gagattcaac gggaattgat tgatctggaa gatgaggaat atttacaagg
     6721 aatcgaaaaa atccatcagg caaaaacggt gcaagaactt cagcagctac ttctgtcatt
     6781 catcaaaaag catcagagac agaaaaagta cagtgacaat gtggaaaaag taatcgtaat
     6841 cctgcaccag cattatcaag aaccacttac actcaaagaa gtgtcagaaa acctgcattt
     6901 aaatgtcatg tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta
     6961 tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta atcaaaacat
     7021 caatgagata gcaagtgaga ttggctacaa caacaccacg tatttctcta aattattcaa
     7081 gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac agtggagaat cataaaagaa
     7141 aaagccaatg taatgaaata acttataaaa aattgtctag aaatctaatt ttgggctgtt
     7201 cttattactt gaggcaaggc agctttttca tgacctctcg ataaacgg
//
LOCUS       DAZKOM010000120         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000120 DAZKOM010000000
VERSION     DAZKOM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.124"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8NF_002552"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8NF_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0472978.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8NF_002553"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8NF_002553"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0472979.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8NF_002554"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8NF_002554"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0472980.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8NF_002555"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8NF_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0472981.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8NF_002556"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8NF_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0472982.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG8NF_002557"
     CDS             complement(4433..5290)
                     /locus_tag="ACG8NF_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0472983.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8NF_002558"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8NF_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0472984.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAZKOM010000121         6967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000121 DAZKOM010000000
VERSION     DAZKOM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.125"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..203)
                     /locus_tag="ACG8NF_002559"
     CDS             complement(<1..203)
                     /locus_tag="ACG8NF_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0472985.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(640..900)
                     /locus_tag="ACG8NF_002560"
     CDS             complement(640..900)
                     /locus_tag="ACG8NF_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472986.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            1074..1178
                     /locus_tag="ACG8NF_002561"
     CDS             1074..1178
                     /locus_tag="ACG8NF_002561"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFQ0472987.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="ACG8NF_002562"
     CDS             complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="ACG8NF_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0472988.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1807..2706)
                     /locus_tag="ACG8NF_002563"
     CDS             complement(1807..2706)
                     /locus_tag="ACG8NF_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0472989.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2719..3312)
                     /locus_tag="ACG8NF_002564"
     CDS             complement(2719..3312)
                     /locus_tag="ACG8NF_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0472990.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(3388..3537)
                     /locus_tag="ACG8NF_002565"
     CDS             complement(3388..3537)
                     /locus_tag="ACG8NF_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472991.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(3553..5757)
                     /locus_tag="ACG8NF_002566"
     CDS             complement(3553..5757)
                     /locus_tag="ACG8NF_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFQ0472992.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(5768..>6967)
                     /locus_tag="ACG8NF_002567"
     CDS             complement(5768..>6967)
                     /locus_tag="ACG8NF_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0472993.1"
                     /translation="DFILKNEDKTERVENLHFETEKYKDHPVDYQPLTEKGRNYLKEE
                     RKLPEWLIDYAEKEGLIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQ
                     GTFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQL
                     NDTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMG
                     AVDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTN
                     QLVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKG
                     DINAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQ
                     GRQQERE"
BASE COUNT         1763 a         1425 c         1212 g         2567 t
ORIGIN      
        1 cgtttcttta ctttctcatc aatcaaaaca taatcgccat aagacaaggt atattttttc
       61 ccgcatgttg gacattgata caaacgatag cttttgatac atttcgctct ttctccacaa
      121 tcgcattcga cagtatgtgg tgcatgatag tttgggaata attcgctgaa gggaacaccc
      181 caataatcaa atttctttcg catgattgat tctgcctcct cttaggaaag gttttatttc
      241 ctttcgttag ttctttcttt attgatttgt tggccagaat gaatcaacga aaagcgacaa
      301 ggacaagggc gcgcagcgtg ccatttaccc ttgtccttgt cgctttttgg tgataaaagg
      361 caacttgttg ccggtctggc aaatcaataa agagatagcg aacaggaacg aaccttttgg
      421 tgagaggaag ttttttctat tttgtcttgc tttgcaagac accatatctt agtgaacgtg
      481 caacgttcac ttcctttctt ctcgaacggc ggtggcggtt ggagtgagag aaaagttttt
      541 caattgagaa ggcacttgtg tcttcctaaa aaggtctttt cctgtttcta tgtaaaacaa
      601 aaagagctgc aatcctcatt taggactgca gctcttcttt tatatgccca tcgatcgcgt
      661 aaaatgatcg gggtttctaa tttgcatcga ttcaatgact aattgattcc gtttattgaa
      721 atgacctgtg acggcaatat tgtattttcc atcatccata attaacaatt tatcgataat
      781 ttcaatatct gcaactacac aattaatcga ttcatgaatt gtttgtaaag taaagcgaac
      841 caataatggt tgtgttttta ttactcggat tttatcaagc aaaccaacat atttgttcat
      901 cacgatcacc tcgctaaaat tatagcaaaa acagctaaaa ttgagaattt ttattcgatt
      961 ttagatatgc tataatgatc aagacagtaa agtttgtgac ggtggctatc cgattgaaag
     1021 tgaggtggtg cgtatgtgcg tacttccaaa atcttacaga aaggagaagc cttttgcctg
     1081 ttgaaacggc gctaggatta atgattagtt tcggttcttt gatcgcaaca atcatttttg
     1141 gaatcttagc agttgtaaac aacaatcaaa aaaaataacc gtcagcttta gccggtcgac
     1201 gattattttt caaaaataat attaagctgg tcaccgtctt aaacggtgct gttaggggag
     1261 ttgttagcgc aactcctctt ttcgtatcca ttataacaac tttattgatc ttttacaatc
     1321 agaatggtaa atcatcatca ctaatatcga ttgattgtcc gccaaatgga ttttgtgaag
     1381 cgttcaatcc attgttgtta ggcgtagcta catcccgttg ttcaaaggtg gtagggcttg
     1441 gactgctgga cgattgatca gcgtgcgcac ggctttctgt cgctgcttta ctttctaata
     1501 gttggaaatt atcggccaca acttctgtca catacacacg ttgtccttgt tggttatcgt
     1561 aggttcgggt ttgaatccgt ccaacaacgc cgatcaaact gcccttttta gctaatgtcg
     1621 ccatcgtttc tgctggtttg cgccaaatca cacagttaat aaaatcagct tctcgattgc
     1681 cgttttgatt cgtgaaattt cggttgacag ctagggtaaa agtcgctact gcagtaccac
     1741 tcgcggtata acgtaaatct ggatctttgg ttaagcgtcc tactaaagtt acattattga
     1801 tcatctttat ctctccattt cttgattcat ttgttgtgat cttttaaact gccgttttct
     1861 ttcttgattt tcaattttga tcacactttt cattaggtga tcaatttcgt tgactgtatc
     1921 tgtaaatgaa atggaattcg caaacgtgtt aactgagcga tatttttccc actgcttttc
     1981 caaatagtca tttcctctga tcttatccat ttctctttca taataaaagg acaacggttt
     2041 tgtatcccca cgttttgtaa atgtatttcg aacacttttg tataactctt tcgtatcgat
     2101 attttctagt ttcggaatgg taaataagtc gtagtaatct tttgctctcg aattatctgt
     2161 gccaaagctg aacgtagcac ttagcttttc tgcaataatt tgttctgttg gataagctgg
     2221 aatctccact tttttatctt caaacattaa ttggtgatag tgtgtatcta cttcaggata
     2281 aatagaatct cctgttgtca cgtccatttt aaacggtaca cgtactttct ctaaattagc
     2341 caccaattta atttgaaatc caggataaaa atcggcttct cttgtttcct tcattccttg
     2401 aatagagaaa acaatgcctt cttttgtggg ttttctagtt aaatcattaa aaatgctctt
     2461 caatttttct tcagttaatt tgctatttct aaatgtcgta tcgatatcaa ttgttgtcct
     2521 tctatcgatt ccatattttg atcctaataa aaagccgcct ttcaaaatga agtcattttt
     2581 gtactttgat tcagaaattt gttcaattag acgatccatc ataaaatgcc gttgcactaa
     2641 ctgtgcattt aatcctgtac tctttgctaa atttcttgtt tttgctgcaa attgtgtcgg
     2701 tgtcatcttt ttcctccatc aatataagct ttgcatgtgg tatttcattt ctttttctac
     2761 atgtagtttt gtcatgtaat ctcttaattt acttggatct ctcaatgggt cttccatgta
     2821 ctcttttaat gcttctaatt tcatttcata ttcaaattca tatctcggat tccaaatatc
     2881 acaaagtgta cgctctttat catacatagt aataggattt ccttgaaagg aagttccctc
     2941 tacttttcct agttcataaa cttctttctt cacgaaatga aattgtaact gctgctcttt
     3001 tgtaattgaa cgatcaacgt tatagccatg gggaaaagtt aagtgaatga atttcggcat
     3061 attcgtagaa agctggtaaa tatctaaagt cgtttcatga gagaagattc ccctggaaaa
     3121 tcgttgctga agccaaaata aatcatctat tccatgacca tcctctacat aataaccacg
     3181 atcaactttg atcaattttc cttgattcac caatctttgt acagtggttg ctgcgattcc
     3241 aaatttcctg gcttccttca tagataaatt tccatcgcga atagccaaca attctttcag
     3301 tttatcttgc atttacttcc cctcccttcg ttacaaatac actacatcaa atatatcata
     3361 gtagtttatt tgtaacaaga ttaacgtcta tcgatctaat tcgatttctt tttgaatgcg
     3421 ctgctggttc tttaattgtt gcagtcttct tttatttgca cgtaatttct cttgttctaa
     3481 acgaattttt gctttttgat tttcgatttc ttgttgtttg tgtgttgtat ttcgcatagg
     3541 ataacccctt cattattttg tagtgcggcg tgttcttttc ttatagttcg tttttttctt
     3601 ttcagaaaat tcaaagcctg ttgtaaagtc ttcttttaaa acgatcgttg cttcaaacga
     3661 acctttctga ccttttagtc ctctgactga ttggcttgtt cggccgtttt gaatgatctc
     3721 tttcagttgt gtggctgtga gtttcttttt ggcaatcgtt gtccataatt taaaatcaca
     3781 ggcttcagac gtacatgccg ccacattttg ataaagcaag acttcttgtt cccgacattt
     3841 tggacaaaca cctaaatgac gcgttgcttc ctgtttttca ctggctgctt tcacctcttg
     3901 taacgttgat ccaaaatctg ttgactggat ttgtcccggt acgacttcca acaagtgaga
     3961 gacaaatttt tggatattag ctaagaaagt agcttgtgtt cctttctgct caccaatctt
     4021 taataagtaa ctttcccatt tggcggtcat ttcggcactt gttagcagcg gctctttttc
     4081 cacggcttgg caaagtaaaa tccctttatt tgtcaccaca agcttatttt tttctacctt
     4141 gatatattcc ttttgtttca aagtttcaat aatatttgct cgtgttgctt ctgtaccgat
     4201 tccttctact tctttcaata tggattgtgc ttcttcgcta tccacggttt ttccagctgt
     4261 tttcatcgct gtgatcaacg taccttcggt ataaggttgt ggtggcttcg tttctttttc
     4321 aaggacttgt agatctaccg tcaccgcttc tccttttgtc actttaggaa gtagcggttc
     4381 ttcttccttt tcttttctgt cgctctcttt gccaaacagc tttttccaac ctagatctac
     4441 tggtactttt ccgactgtct ttagctgaag ctgctgaact tgcgtcaaga tcgttgtttc
     4501 ttcataggta tacttttccg caaacatggc caccgttgtt tttaacactt gttcataaat
     4561 agcctgctgc agtggactta attgttcaaa agccgctgcc gtcatgaccg tctttgtagg
     4621 aatgatcgcg taatgttcct ggactttctt attattcaca tatcgactat tcggtgtatg
     4681 aataggagta ggaatggctt cggcctttaa aaagtgtttg tattcgtcta agtgatccaa
     4741 cagatacgca tactcccctt ctgtaatata aggggtatct gttcgtgggt agcttagata
     4801 tttcccttca tacaaccctt gcatggcttc caaagtatct ttggcactag ctttcatgag
     4861 ctgattcatt ttagattgaa ggctggatag agaaaaaaga cgtggacttg ccgcactttt
     4921 tttctcggtt tcaacttggt ggatcgtacc tggctgtttt cccaattgaa cccccttttc
     4981 ttttaaataa tcttcaaatg cttcttgcgt cgcttgggtt tcattcggat caagcttcgc
     5041 atcaaatgcg ccattttgag cgattacttg ggcttttcct tcaaaatatg gttctttttg
     5101 gaagtttcta atcttttcct gtaattgata aatcatgtaa agcgttgggg tttggactct
     5161 gcctaaggaa aaacttcctg gtatgccttt ttgctgcaag agtaaactat acagcgggct
     5221 accgttcatg ccgatcaacc aatcggcgat ctggcgtgct tgtgcttctt caaattttgg
     5281 gtaggtctct tctgcatctc gtaactgctg gaatccctga taaatggctt ctttttctaa
     5341 ggagttgatc caaagtcttt taaatatctt tccatgatcg gcgcctgctt gttgaatgat
     5401 cgtccaagct atcagttccc cttcacgatc gctatctgtc gcaataataa tggtgtcggc
     5461 cgattgaagt tgctgtttca ccaccttgaa ttgctttttc ttatctttag gaacatgata
     5521 gtgataatgt gtagggaaaa taggcaaatg ctctaatgac cattgtttcc aattttcatc
     5581 gtacatatcg ggcgaatcta actcgactaa atgaccaaaa ccatacgtga tcacagtttc
     5641 tcctgaaaac aatcgatctt ggatctcaaa atacccatct tttcgggtag ctttagaaaa
     5701 gctatcggca taagccttcg cttgtgacgg tttttctgct aaaataacgg ttttcatcat
     5761 gacgatttca ctccctttct tgctgtctac cttgttgaaa caacatggca cgtttttgct
     5821 ttctgatctc ttgttggtag atttcatgct tcaattgatc attccaatcc ttttttactt
     5881 caggtacaaa ttcatgttca tgatttttat agcctttgta gtattgttct gctttataag
     5941 agaaaagaat ctgagcatta atatcccctt tttcttgata aaaacgatca aaatcgtacg
     6001 taccatccac gcgttcacaa gcttttaatt cttttgcgac ttctctacaa atatctttca
     6061 aattaatagc ttctattgaa tcgttaacag aaagctgctg accaaaagac gcatttactt
     6121 tgtgagcgga aaccaattgg ttcgttaatt gcagattgtt ttctgtcttg tgtatggcca
     6181 taatagcttc tggtgtgatc ttttcttctt ttgcgacttc ttcataaatg actttgtatt
     6241 cttttggcac ttgccaatca ttagcgattc ctcgttcaca acgtactttc tgattcgtaa
     6301 atggatcaac tgcgcccatt aattgttctt tttcgtagaa aatatgtcct gcacggtcat
     6361 tatccacaca aatctcgatg gattgaggaa aggcttgttt ctttctcaat tcagatactg
     6421 cctcaccaaa ataatgactg atcacatggt gttttaaccc ttccatcgaa actaaccacg
     6481 tatcgtttaa ctgatcacga tttagtgcag catagctaag taaatcaata ctactctcaa
     6541 aaaattttaa ctgcttaggg tcaccgatct ttaggttaaa gccatgattc gctgtcgaat
     6601 ttttatctat atgcttatat gtgcctctct caccaaaacg ttcataatca atgaacgttc
     6661 cttgataact cgcccctact gtctcatttg tctgtggatc tttccataaa aaggcgaccg
     6721 catgatctaa acgatcgtct cgaactaaga aattttgccg ttcatgtttt ggctttagtt
     6781 cggcaatcaa cccttctttt tctgcataat caatcagcca ctctggtagt tttcgttctt
     6841 ccttcagata attccttcct ttttctgtga gtggttgata atcaacgggg tgatctttgt
     6901 acttttctgt ctcaaaatga agattttcta ctcgttctgt tttgtcttca ttctttagaa
     6961 taaaatc
//
LOCUS       DAZKOM010000122         6919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000122 DAZKOM010000000
VERSION     DAZKOM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.126"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1200
                     /locus_tag="ACG8NF_002568"
     CDS             <1..1200
                     /locus_tag="ACG8NF_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0472994.1"
                     /translation="DFILKNEDKTERVENLHFETEKYKDHPVDYQPLTEKGRNYLKEE
                     RKLPEWLIDYAEKEGLIAELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQ
                     GTFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQL
                     NDTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMG
                     AVDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTN
                     QLVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKG
                     DINAQILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQ
                     GRQQERE"
     gene            1211..3415
                     /gene="topB"
                     /locus_tag="ACG8NF_002569"
     CDS             1211..3415
                     /gene="topB"
                     /locus_tag="ACG8NF_002569"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0472995.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            3431..3580
                     /locus_tag="ACG8NF_002570"
     CDS             3431..3580
                     /locus_tag="ACG8NF_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0472996.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3654..4247
                     /locus_tag="ACG8NF_002571"
     CDS             3654..4247
                     /locus_tag="ACG8NF_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0472997.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            4263..5162
                     /locus_tag="ACG8NF_002572"
     CDS             4263..5162
                     /locus_tag="ACG8NF_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0472998.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            5165..5650
                     /gene="ssb"
                     /locus_tag="ACG8NF_002573"
     CDS             5165..5650
                     /gene="ssb"
                     /locus_tag="ACG8NF_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0472999.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            6016..6276
                     /locus_tag="ACG8NF_002574"
     CDS             6016..6276
                     /locus_tag="ACG8NF_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473000.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            6717..>6919
                     /locus_tag="ACG8NF_002575"
     CDS             6717..>6919
                     /locus_tag="ACG8NF_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0473001.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT         2513 a         1207 c         1431 g         1768 t
ORIGIN      
        1 gattttattc taaagaatga agacaaaaca gaacgagtgg aaaatcttca ttttgaaaca
       61 gaaaagtaca aagatcaccc cgttgattat caaccactca cagaaaaagg aaggaattat
      121 ctgaaggaag aacgaaaact accagagtgg ctgattgatt atgcagaaaa agaagggttg
      181 attgccgaac taaagccaaa atatgaacgg caaaatttct tagttcgaga cgatcgttta
      241 gatcatgcgg tcgccttttt atggaaagat ccacagacaa aagagacagt aggggcgagt
      301 tatcaaggaa cgttcattga ttatgaacgt tttggtgaga gaggcacata taagcatata
      361 gataaaaatt cgacagcgaa tcatggcttt aacctaaaga tcggtgaccc taagcagtta
      421 aaattttttg agagtagtat tgatttactt agctatgctg cactgaatcg tgatcagtta
      481 aacgatacgt ggttagtctc tatggaaggg ttaaaacacc atgtgatcag tcattatttt
      541 ggtgaggcag tatctgaatt gagaaagaaa caagcctttc ctcaatccat cgagatttgt
      601 gtggataatg accgtgcagg acatattttc tacgaaaaag aacaattaat gggcgcagtc
      661 gatccattta cgaatcagaa agtacgttgt gaacgaggaa tcgctaatga ttggcaagtg
      721 ccaaaagaat acaaaatcat ttatgaagaa gtcgcaaaag aagaaaaggt cacaccagaa
      781 gctattatgg ccatacacaa gacagaaaac aatctgcaat taacgaacca actggtttcc
      841 gctcacaaag taaatgcgtt ttttggccag cagctttctg ttaacgattc aatagaagcc
      901 attaatttga aagatatttg tagagaagtc gcaaaagaat taaaagtttg tgaacgcgtg
      961 gatggtacgt acgattttga tcgtttttat caagaaaaag gggatattaa tgcgcagatt
     1021 cttttctctt ataaagcaga acaatactac aaaggctata aaaatcatga acatgaattt
     1081 atacctgaag taaaaaagga ctggaacgat caattgaagc atgagatcca gcaacaagaa
     1141 atcagaaagc aaaaacgtgc catgttgttt caacaaggca gacaacaaga aagggagtga
     1201 aatcgtcatg atgaaaaccg ttattttagc agaaaagccg tcacaagcga aagcttatgc
     1261 cgatagcttt tctaaagcta cccgaaaaga tgggtatttt gagatccaag atcgattgtt
     1321 tacaggagaa actgtgatca cgtatggttt tggtcattta gtcgagttag attcgcccga
     1381 tatgtacgat gaaaaatgga aacaatgggc attagaacat ttgcctattt tccccaatca
     1441 ataccactat catgttccta aagataagaa aaagcagttt agtgtcgtca aacgacagct
     1501 gcagtcagct gatacgattg tgatcgcgac tgatagcgat cgtgaagggg agctgattgc
     1561 ctggtcaatc attcaacaag caggcgccaa tcaaggaaaa acctttaaaa gactttggat
     1621 caactcctta gaaaaagaag ccatttatca gggattccaa cagttacgag acgcagaaga
     1681 gacctatcca aaatttgaag aagtacaagc acgtcagatc gccgattggt taattgggat
     1741 gaacggtagc ccactgtata gtttactctt gcagcaaaaa ggcataccag gaagtttttc
     1801 tttaggtaga gtccaaaccc caacactcta tatgatttat caattgcagg aaaagattag
     1861 aaacttccaa aaagaaccat attttgaagg aaaagcccaa gtaatcgctc aaaatggcgc
     1921 atttgatgcg aagcttgatc caaatgaaac ccaagcgaca caagaagcat ttgaagccta
     1981 tttaaaagaa aaaggtgttc aattaggaaa acagccaggt acgatccttc aagtcgaaac
     2041 agagaaaaaa agtgcggcta gcccacgtct tttttcttta tcgagccttc agtctaaaat
     2101 gaatcaactc atgaaagcta gtgccaaaga tactttggaa gccatgcaag ggttgtatga
     2161 aggaaaatat ctaagctatc cgcgaacgga taccccttat attacggaag gggaatatgc
     2221 gtatctgttg gatcacttag acgaatacaa gcatttttta aaagccgaag cgattccgac
     2281 tcctattcat acaccgaata gtcggtatgt gaataataag aaagtccagg aacattacgc
     2341 gatcattcct acaaaaacgg ttatgacagc agcggctttt gaacaactaa gtccactgca
     2401 gcaggctatt tatgaacaag tgttaaaaac aacggtggcc atgtttgcgg aaaagtatac
     2461 ctatgaagaa acaacgatct tgacgcaagt tcagcagctt cagttaaagg caatcgaaaa
     2521 ggtgccgcta gatctaggtt ggaagaagtt atttggaaaa gagagcgacg gaaaagagaa
     2581 ggaagaagag ccgctacttc ctaaagtgac aaaaggagaa atggtgacgg tggatctaca
     2641 agttcttgaa aaagaaacca agccaccaca gccttatacc gaaggtacgt tgatcacggc
     2701 gatgaaaaca gctgggaaaa ccgttgatag tgaagaagcg caatccatat tgaaagaagt
     2761 ggaaggaatc ggtacagaag cgacacgggc gaatattatt gaaaccttga aacaaaagga
     2821 atatatcaag gtagagaaaa ataagctcgt ggtgacgaat aaagggattt tgctttgcca
     2881 agccgtggaa aaagagccgc tgcttacgag tgccgaaatg accgccaaat gggaaagcta
     2941 cttgttaaag atcggtgaac gaaaaggaac acaaaccact tttctagcca atatccaaaa
     3001 atttgtctct cacttattgg aagtcgtacc agggcaaatc caatccactg attttggatc
     3061 gacgttacaa gaagtgaaag cagccagtga aaagcaagaa gccgcgcgtc atttaggaat
     3121 ttgtccaaaa tgccaggaac aagaagtctt actctatcaa aaggtggcag catgtacgtc
     3181 tgaagcctgt gatttcaaac tatggacaac gattgccaaa aagaaactta cagcgacaca
     3241 gctgaaagag atcattcaaa atagccgaac aagccaacca gtgaaagggt taaaaggtca
     3301 gaagggttcg tttgaagcaa cgattgtttt aaaagaagac ttcactacag gctttgaatt
     3361 ttctgaaaag aaaaaaacga acttcaagaa aagaacacga cgcactacaa aataatggag
     3421 ggttaatctt atgcgtaata caacacacaa acaacaagaa atcgaaaatc aaaaagccaa
     3481 aattcgttca gaacaggaaa aaatgcgtgc cagcaaacga agactgcagc aattgaagaa
     3541 ccagcagcgg attcaaaaag aaatcgaatt agatcgataa attcctttct tgttaatata
     3601 aaactaacat gttatagttg tgttagtttt atattaactt taggggaaga gtaatgaaag
     3661 acaaaatcca atgtattttt gataaacata atggcaacct atccttgaag gatgctagaa
     3721 gtgaaggatt atcgcctatg acgttagatc gattagccga aaaggataaa atagaaaagg
     3781 tagcaccagg tttctatacc ttaaaggatg cgttcgtgga tgaattgtat ctcgctcaga
     3841 gtatctattc cagagggatt ttttctcatg agactgcgct cgatcttttt caagtaggaa
     3901 catatatccc taaaaagatc aatatgatgt ttcctaaagg atacaatgta tcaaaggaga
     3961 aattagatca gtatgccatt caaccgcatt acactaataa agaaaatttt gacctgggag
     4021 tcacagaaac agaaagcttt tacggcaata aaattttagt ctatgattta gagcgaacgt
     4081 tatgtgacat gtggaatcca agatacaaag caagcgtaga ggtaaaacaa gaagcattaa
     4141 aagaatatat gacaagtacc agtagaaata caaataagct aagaagatac atggatattt
     4201 taaaatcacc aaaagaaatg actttgtata tgttgccact ttattgagag gagtaaagat
     4261 gaatgacacc gacacaattt aaagagaaaa caagaaattt cgcagcagag aaacatttaa
     4321 acccacaaat tgtccaaaga tattatatga tggaaaaatt attagaatgt attgttgaat
     4381 cgccttatca tgatgatttt gtattaaaag ggggatttct gattggatca aaatacgggt
     4441 tagaaaaccg ttcaaccgtt gatattgata cgacttatcg aaattcgaaa ctaacaaaag
     4501 aaaagttaga aactgttttt tctgaattaa cagctaatcc tacaaaagat ggaattcgtt
     4561 ttgaattaaa gggattaaca gagacgaggg aagccgatta ttatccaggt tttcaagcaa
     4621 agtttattgc ttatcttgaa aatgcacgtg taccttttaa attagatatt acttcaggtg
     4681 attctattat tccagcagca ttgatgtatg aacacaaatt aatgtttgaa gataaaacga
     4741 ttcatattcc agcttatcct acggaacaaa ttctagctga aaaattacat gccacgttta
     4801 gttttggaaa agataattcg cgcatgaaag atttttatga cctgtatatg attccgaaat
     4861 tagaacgtat cgatgagaaa aatttgtata agagtgttca aggaacattc aaggaaagaa
     4921 ataatcaatc ggtgtttaag catttgtatg atcaggaaat gcctcaattg gtgaaagatg
     4981 agggcttgag agaaaagtgg aaaagatacc aacaagaaaa ctattttgcc aaagacattt
     5041 ctttcgaatc tacactaact tccgttgatt ccctgatgca atcaattgta gagcaagaga
     5101 aggcagaacg aaaacaaaat tttaagagat cacaacaaat gaatcaagaa atggagagat
     5161 aaagatgatc aataatgtaa ctttagtagg gcgattaact aaagatccag atttacgtta
     5221 taccgcaagt ggcacagcag tggcgacttt ttccctagcc gttaatcgta atttcacgaa
     5281 tcaaaacggc aatcgagaag ctgattttat caattgcgtg atttggcgca aaccagcaga
     5341 aacgatggcg acattagcta aaaaaggcag cttgatcggc gttgtcggac gaattcaaac
     5401 acggacttat gacaaccagc aaggacaacg tgtgtatgtg acagaagttg tggccgataa
     5461 cttccaacta ttagaaagta aagcagcgac agaaagccgt atgcacgctg atcaatcgtc
     5521 cagcagtcca agccctacca cctttgaaca acgggataca gcgacgccta acaacaatgg
     5581 attgaacgct tcacagaatc catttggtgg acaatcaatc gatattagcg atgatgatct
     5641 accgttctaa gtagcatttt ctattggttg catttgctac ggtttatgtt ataatttaga
     5701 caacaaaaag aattttcaat ctataaccaa aaaccactag ctggcgggct agtggctttc
     5761 ttttacgttt ctattataag aattaacggg taggcttact gtctttcttg gcaaatttac
     5821 aatcaagcca tttgcagata tagtgcgcgg ctacttctgc catgattgca aaaacaaaat
     5881 caaaaagaat tttcaaacct ataacctcct ccccttgcca gattaggccg gggtagcaga
     5941 cagcaagact attatagcat aattgaaata tccaaatgat tggatttttg ctataatttt
     6001 agcgaggtga ttgtgatgaa caaatatgtt ggtttgcttg ataaaatccg agtaataaaa
     6061 acgcaaccgt tattggttcg ctttacttta caaacgattc atgaatcgat taattgtgta
     6121 gttgcagata ttgaaattat cgataaattg ctaattatgg atgatggaaa atacaatatt
     6181 gccgtcacag gtcacttcaa taaacgaaat cagctagtta ttgctagcat gtatgttaga
     6241 aatcccgatc attttacgcg atcgatgggc atataaataa aagaagagtt gtagtcctaa
     6301 atgaggattg cagctctttt tgttttacat agaaacagga aaagaccttt ttaggaagac
     6361 acaagtgcct tctcaattga aaaacctttc tctcactcca accgccaccg ccgttcgaga
     6421 agaaaggaag tgaacgctgc acgttcacta agatatggtg tcttgcaaag caagacaaaa
     6481 tagacaaaac ttcctctcac caaaaggttc gttcctgttc gttatctctt tattgatttg
     6541 ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc
     6601 acgctgcgcg cccttgtcct tgtcgctttt cgttgattta ttctggccaa caaatcaata
     6661 aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaaacatgc
     6721 gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac tatcatgcac
     6781 cacatactgt tgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tatcgtttgt
     6841 atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattac attttgattg
     6901 atgagaaagg aaagaaacg
//
LOCUS       DAZKOM010000123         6896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000123 DAZKOM010000000
VERSION     DAZKOM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.127"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8NF_002576"
     CDS             complement(<1..182)
                     /locus_tag="ACG8NF_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0473002.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8NF_002577"
     CDS             517..951
                     /locus_tag="ACG8NF_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0473003.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8NF_002578"
     CDS             974..1294
                     /locus_tag="ACG8NF_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473004.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8NF_002579"
     CDS             1435..1626
                     /locus_tag="ACG8NF_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473005.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8NF_002580"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8NF_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0473006.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8NF_002581"
     CDS             2112..2375
                     /locus_tag="ACG8NF_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473007.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8NF_002582"
     CDS             2399..2587
                     /locus_tag="ACG8NF_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473008.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8NF_002583"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8NF_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473009.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8NF_002584"
     CDS             3070..3441
                     /locus_tag="ACG8NF_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0473010.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8NF_002585"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8NF_002585"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8NF_002586"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8NF_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473011.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8NF_002587"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8NF_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0473012.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8NF_002588"
     CDS             5250..5447
                     /locus_tag="ACG8NF_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473013.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8NF_002589"
     CDS             5553..6035
                     /locus_tag="ACG8NF_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473014.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8NF_002590"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8NF_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0473015.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2333 a         1059 c         1355 g         2149 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttga
//
LOCUS       DAZKOM010000124         6786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000124 DAZKOM010000000
VERSION     DAZKOM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.128"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..653)
                     /locus_tag="ACG8NF_002591"
     CDS             complement(195..653)
                     /locus_tag="ACG8NF_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0473016.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(666..1997)
                     /locus_tag="ACG8NF_002592"
     CDS             complement(666..1997)
                     /locus_tag="ACG8NF_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0473017.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(2008..3450)
                     /locus_tag="ACG8NF_002593"
     CDS             complement(2008..3450)
                     /locus_tag="ACG8NF_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0473018.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3437..4201)
                     /locus_tag="ACG8NF_002594"
     CDS             complement(3437..4201)
                     /locus_tag="ACG8NF_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0473019.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4305..5180)
                     /locus_tag="ACG8NF_002595"
     CDS             complement(4305..5180)
                     /locus_tag="ACG8NF_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0473020.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5435..5519)
                     /locus_tag="ACG8NF_002596"
     tRNA            complement(5435..5519)
                     /locus_tag="ACG8NF_002596"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5483..5485),aa:Leu,seq:caa)
     gene            complement(5537..5608)
                     /locus_tag="ACG8NF_002597"
     tRNA            complement(5537..5608)
                     /locus_tag="ACG8NF_002597"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5574..5576),aa:Gln,seq:ttg)
     gene            complement(5618..5690)
                     /locus_tag="ACG8NF_002598"
     tRNA            complement(5618..5690)
                     /locus_tag="ACG8NF_002598"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5655..5657),aa:His,seq:gtg)
     gene            complement(5752..5836)
                     /locus_tag="ACG8NF_002599"
     tRNA            complement(5752..5836)
                     /locus_tag="ACG8NF_002599"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5800..5802),aa:Leu,seq:caa)
     gene            complement(5857..5927)
                     /locus_tag="ACG8NF_002600"
     tRNA            complement(5857..5927)
                     /locus_tag="ACG8NF_002600"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5893..5895),aa:Cys,seq:gca)
     gene            complement(5973..6044)
                     /locus_tag="ACG8NF_002601"
     tRNA            complement(5973..6044)
                     /locus_tag="ACG8NF_002601"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6010..6012),aa:Gln,seq:ttg)
     gene            complement(6056..6128)
                     /locus_tag="ACG8NF_002602"
     tRNA            complement(6056..6128)
                     /locus_tag="ACG8NF_002602"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6093..6095),aa:His,seq:gtg)
     gene            complement(6131..6204)
                     /locus_tag="ACG8NF_002603"
     tRNA            complement(6131..6204)
                     /locus_tag="ACG8NF_002603"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6170..6172),aa:Trp,seq:cca)
     gene            complement(6211..6291)
                     /locus_tag="ACG8NF_002604"
     tRNA            complement(6211..6291)
                     /locus_tag="ACG8NF_002604"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6255..6257),aa:Tyr,seq:gta)
     gene            complement(6293..6368)
                     /locus_tag="ACG8NF_002605"
     tRNA            complement(6293..6368)
                     /locus_tag="ACG8NF_002605"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6333..6335),aa:Phe,seq:gaa)
     gene            complement(6404..6476)
                     /locus_tag="ACG8NF_002606"
     tRNA            complement(6404..6476)
                     /locus_tag="ACG8NF_002606"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6441..6443),aa:Asp,seq:gtc)
     gene            complement(6486..6558)
                     /locus_tag="ACG8NF_002607"
     tRNA            complement(6486..6558)
                     /locus_tag="ACG8NF_002607"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6523..6525),aa:Val,seq:tac)
     gene            complement(6574..6645)
                     /locus_tag="ACG8NF_002608"
     tRNA            complement(6574..6645)
                     /locus_tag="ACG8NF_002608"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6610..6612),aa:Glu,seq:ttc)
     gene            complement(6660..6749)
                     /locus_tag="ACG8NF_002609"
     tRNA            complement(6660..6749)
                     /locus_tag="ACG8NF_002609"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6713..6715),aa:Ser,seq:gga)
BASE COUNT         2062 a         1485 c         1093 g         2146 t
ORIGIN      
        1 tatattctct taaataatca aatttatttt taagaatcct gaaaataagt tttctaaatc
       61 ataggagcaa caaaaaaatt cgataattaa caaaaatcaa aatgattaaa tctagtgatt
      121 gaaacatatc atttcaaaaa aattttgtaa attcacccat aaatatcacc ctatttaact
      181 tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc ttggttttgt
      241 tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt atctttttgt
      301 gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt tacctcctgg
      361 ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc taaacccata
      421 tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg ggtaggaaat
      481 atattatcga ttattccttt aactggcgca tggatatatc catcatgatt cgttatagcg
      541 tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt taatggaata
      601 atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg cactttttat
      661 cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc atctagtatt
      721 ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg taaagctttt
      781 tgataagaac cttcaaagta agcatctact agtaacttct ctactgcaac ttgtgcctcc
      841 atcaatcctt tatgaaaatc actgattggt tttcttagtg taattggctc cacaccagtt
      901 gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg aatcgcccct
      961 tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga aatagctata
     1021 tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc cacaataccg
     1081 cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat gaccatatta
     1141 gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa gtaatactct
     1201 aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat catttgaaag
     1261 gttttttgcc aagttttatc tcctatatta aaatcggcga cttgcatttc ttttgttatt
     1321 agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata aatcgatgta
     1381 taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg atcataaccg
     1441 taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt aatcattttt
     1501 acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt tgtataattc
     1561 aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa taaacctttg
     1621 attgagcgca tcccataagc aaatcctccg agtccacacg tttcttgtcc tactaatcca
     1681 tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac acgaatttgt
     1741 gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc acgaatgcga
     1801 atatttgaaa aaccttttct tttcagcata aatttaacaa tcaagtacat atccatattt
     1861 ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt ttcattgttc
     1921 aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat agtgataatc
     1981 tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga attctgccat
     2041 aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg gtttaatgat
     2101 cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat ccactacctc
     2161 tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc ctccaaacgc
     2221 ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta actctgtttc
     2281 ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg tctgaatgtt
     2341 tctcttattg atcaaataat aaaaagtaac gtaatacaaa gcaaaaaaga aaattccgat
     2401 tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat caacaataca
     2461 tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc
     2521 ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac taaattcaac
     2581 aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga ttcctttgac
     2641 catctcttta ttttctttat aagctgcttt atagatagct agacatatag ctggtatacc
     2701 aaacatcatt actacaaaaa aaactgataa aaacagtcca gcctgaggat ctccgttgat
     2761 aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc ttcctaaatc
     2821 gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca aacgattcaa
     2881 aaacataaat atccccacac ctaatgctcc catatcaacg agcagttttg caaaattatc
     2941 aatcccaatt tggatgagcg gccagataaa gcccaaaaaa acagctgcga ttgtttgtag
     3001 aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa
     3061 tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa taattccacc
     3121 aaaaatcccc atattcactt ctgggtccat aatttctaag ccttttttca aggtagcaat
     3181 cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa aaccgattgc
     3241 aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag ctgcaccaat
     3301 tactgggatg ttcagcatat cgtcagctga taaacgcatg atcaatcccg ctaatggcat
     3361 cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt taacattcat
     3421 ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa ggatcatttt
     3481 tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgggta cgaggataca
     3541 caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac atgacttcga
     3601 tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc acactcgtat
     3661 tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc tcaaaataat
     3721 cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa tcatattttt
     3781 ttgctaatgt aacaactacc tcctgatttt catccaaagt atgagtatga tgatgactgt
     3841 ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca ttccattcct
     3901 gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag gatctgaact
     3961 ctccgctttc gataagagta tcgacagttt tgaggagtgg tttgccacac gtcaatgtta
     4021 aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagca tgctcgaaac
     4081 ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa gcatccataa
     4141 tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc acttttccca
     4201 tttcattctc cttctttttt ctactaaaat aggcgcaacc aaataatcat agtccattta
     4261 ctatgtgtca tttggttgcg cctatagata gttacgccct tattttaata gattcttgct
     4321 agatgaattg ccaaatagat gatttcattt tcttgaatgg caagttgaaa ttgctgttcg
     4381 atgatatctt taatctttag tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta
     4441 aaaaaaccaa tattttctac tggcgtttct tcctgataaa tcaagcgttt aaccaggaat
     4501 ttgatatgaa ccattaagcg atgataagtc agtgattctt tatcataaac gtttgaatag
     4561 gatttttcta gatgggacaa aatcgtggtc gtcatttcta ctatttttaa gctctttaaa
     4621 ctattaggct ctcctagctc agcattaatg atatgcatag tgatgaatcc tacttcattt
     4681 gaccctaact cggtttgata tcgatgattg attcgctcga ccacttctac tgctatatca
     4741 tattcatttg gataaaggaa tctcatttct ggcaacataa ttgctgtgac atctctatct
     4801 tctctttttc gttgaatagc aaaataaata tgatctgtca atgtaatata gatatttgaa
     4861 tctaatggag aggagatcct agctgttatc agttcaatca cttcttccgc aaaatccata
     4921 aattcgattg gaatctgcga gactatctca ctgaatcgtt tgatcttttc tttagaatct
     4981 aatacaaata atttgtcagc atcctcaaca tcaaatggct tactcacttt agcagaaaag
     5041 ccaatccctt tccccatcgc aattttttct attccatcgt catcgatcaa tacagcatta
     5101 tgattcagct ttttcatcac atacaccgtt aattcacctc ctctatatga taaaacaaaa
     5161 caacttgcag actccttcat acatattttt gtctccaaac aatttttaaa gacaaaaaac
     5221 gacctaagaa agaacccttt cttaggtcgt gcctaattga atagtagcac cctacataat
     5281 ttacaaaaat agtttaacac ataataaaag cgttatcaat atctttttta ctcgttttat
     5341 gataatactt gtatcgaatt gatttggagg taattacata aaaacaaaaa aaatatagca
     5401 actctctata agaaagttgc tatattgata attataccgg cggccggggt cgaaccggca
     5461 cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca
     5521 aatttaaaaa taataactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc
     5581 ccttgcctta ccgcttggct ataccccaag aataaaaggc gaatgatggg aatcgaaccc
     5641 acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catcataaaa
     5701 gatgttattt aatttctcaa taaaaaacta agatgaagac atcttagtca ataccggcgg
     5761 ccggggtcga accggcacgc cctcaacggg cactggattt tgagtccagc gcgtctgcca
     5821 attccgccac gccggcaagt tgatttatta gtactaaggc ggtaaccgga tttgaaccgg
     5881 tgatgaaggt tttgcagacc tctgccttac cacttggcta taccgccatt atactattat
     5941 caatctataa aataaagaat aaattataga aactggggta gctggattcg aaccaacgca
     6001 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga
     6061 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
     6121 acattcgcca tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct
     6181 gttctacctt taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc
     6241 ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg
     6301 cgggacagaa tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct
     6361 accgcgccat attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc
     6421 gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt
     6481 cattatggag gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc
     6541 cagctgagct aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt
     6601 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac
     6661 ggagagtaag ggattcgaac ccttgagaca gtttggcacc atctacatga tttccaatca
     6721 tgctccttcg gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa
     6781 aatgag
//
LOCUS       DAZKOM010000125         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000125 DAZKOM010000000
VERSION     DAZKOM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.129"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8NF_002610"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8NF_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8NF_002611"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8NF_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0473021.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8NF_002612"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8NF_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8NF_002613"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8NF_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0473022.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8NF_002614"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8NF_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0473023.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8NF_002615"
     CDS             3582..4271
                     /locus_tag="ACG8NF_002615"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0473024.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8NF_002616"
     CDS             4285..5787
                     /locus_tag="ACG8NF_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0473025.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8NF_002617"
     CDS             5800..6276
                     /locus_tag="ACG8NF_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0473026.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8NF_002618"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8NF_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKOM010000126         6592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000126 DAZKOM010000000
VERSION     DAZKOM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.130"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..1694
                     /locus_tag="ACG8NF_002619"
     CDS             141..1694
                     /locus_tag="ACG8NF_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0473027.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2075..3859)
                     /locus_tag="ACG8NF_002620"
     CDS             complement(2075..3859)
                     /locus_tag="ACG8NF_002620"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0473028.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3874..5595)
                     /locus_tag="ACG8NF_002621"
     CDS             complement(3874..5595)
                     /locus_tag="ACG8NF_002621"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0473029.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5695..6456
                     /locus_tag="ACG8NF_002622"
     CDS             5695..6456
                     /locus_tag="ACG8NF_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0473030.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2077 a         1191 c         1285 g         2039 t
ORIGIN      
        1 tattaatctt agtatttttt gatgtataaa ctacctgaaa ttgagagtac ataccaaatt
       61 cataaatttt ttattagtgg gactagtgat tgtatggatt ttgacgcata atgaatgaag
      121 tatgagaaaa ggagagacaa atggatcaag aactatttat tatcgaagta ttatcagcta
      181 aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag aataatgttc aagcttttaa
      241 gcttagtatt tttctctggt ttgctttttt tcggagctgt atcgttccta tatcgccata
      301 tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt aaataatttg tatcactttt
      361 cgtggggagt tggcggaata acaattgttg tgcttattgt ttttcttcta cttatttggc
      421 tccgttacca tcaattgtcg cgtcatttcg atcaatggct tagtcaaaga gcacaacagt
      481 ttttacataa taaaccaact tctcaagatg atgactatct ttattttgag aagacttcga
      541 cgagtcaagc ttttcggata caaaaaagtg attgccagtt attgctgaga tcacttgatg
      601 atttacctat ctacttaggc gaaagcaaat caaaattcgg aatgaacaca ttccatattt
      661 ttttactaga aaacgaaccc agatgggaaa aaagccagtc ttcgcctggt ggatacctga
      721 caactaagat gagtacatta gtcactggtt ttttcctcat tcttttagct ggaacaatct
      781 attatgggag atacactcag ccaaaaatgt cttctcatat aaatgctgct tctcacatga
      841 caatgaactc aactaacgat acgcatatac aaaagacaaa tgaagggttg ttagctgatc
      901 ctaagcaaaa ggtaaatttg acaacacaaa agataaatga tctcaaactt gatacagaga
      961 cgcactctct ctatatgaca acagatagtg gtgctaattg ggtatttgtg ccgattgatt
     1021 cgcaatggtt gcgttttggc gaatacaccc tgacaacggg gctgattcct gaaggttatt
     1081 ggatggacaa cacatatgat atttccgccg atttctcatg gtttatctat tcttctgggg
     1141 atgatgtacg tctgctgact tcaagagata atggtaaaac atggcaaacg tctattatca
     1201 cccaatcagt tcagccgata cgttatcgaa aagcggtatt ttatggcaac gatggtctat
     1261 ttatctgtac atcagccagt gggatgtctg cagaaacttt gtacatttac actactatgg
     1321 atggcggaat cacttggata aaaagtggaa gtacaatgat caatcagcca gtacaaaatg
     1381 tcagcttcgt tacaacttct ttaggttttg tttcgacacg tgaaagtttg ttttatacaa
     1441 acaacggagg acgctcattc aaagaatcag ttctgtcgat tccggaaaac tatcagacag
     1501 gggggataga tctttttcaa tcaccaaatg aagttactca aataagcgcg aatacgttag
     1561 agacaaaatt ttatttgttg aaaaaaggac caatcgatgc agggaaaatg tttgcctgcc
     1621 tcttccgttc aactgataac ggggaaacat ggcaattcgt tcagcaacta tcgcaagtag
     1681 aacaaggaga ttaaagaaga gagattgtga gcctgacgtt cagcaccgag tcacaagaaa
     1741 caaattcaaa aaacagcttc tcatatttgt tgaatagtgt aactcgtgat tcggagttga
     1801 caagagcaac aagaagcgat tttgaagtat agcttctcgt attttacaag atcgtgtctt
     1861 attgccgata ggttgtctct tgaacaccgt ttattcgtaa aaagggattg tgacaagtct
     1921 tttgtcacta cttaccgaac gaaactgaag gagctatcca aaaagtatca ttagtattat
     1981 tctttgtgtt tctagtggca ccactagtac atttaaaaaa gctatcgatc atggactaat
     2041 catacatgat cgatagcttt ttagataatg tttgttacgc tatctgaaat tgactattat
     2101 aaagactggc ataaagcgtt ggtgattgga gtagttcaga atgtgttcct ttttcgacga
     2161 tatcgccatt cttcattaca aggatcatgt ctgcattttc aatcgttgac aaacggtgtg
     2221 caatcacaaa acttgtccta tcttttgtca cagcttccat tgcttgctga atcaatgctt
     2281 ctgttcgtgt atctacgcta gaagttgctt catctaaaat aacgacagga ggattcgcta
     2341 gaatgactcg agcaattgtc aataattgtt gctgtccttg agataacgtg ccgttttcgc
     2401 tagaaatgat cgtatcatat ccgtctggca gtgttcgaat aaagtgatca cattgtgcga
     2461 tttcagccgc ttcgataatt tgttctcttg tggcatccat tttcccgtag gcgatattat
     2521 cagctaccgt tccttcaaac agccaggtat tttgtaaaac catcccaaaa gaacgacgta
     2581 gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa ttcgatcttt cctttattca
     2641 gttcataaaa acgcatcagc agattgacta aagtcgtttt tcctgcacca gttggaccaa
     2701 cgatcgctac cgtttgtttt ggctttactg aaaaatcgac ttgattcatc aatattttat
     2761 ctgatgtgta accaaactgt acatttttga aagctatttt tcctttcggt tgttctaaaa
     2821 gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc taatatctca aatatcctat
     2881 cgattgctgc cattgcggat tggatcgagt tgatcacata agaagcggta gaaattggtt
     2941 cagaaatctg attgatgtac tgaagataag cttgtaagaa gccgatcgtc acaccacctg
     3001 atagaacaag catagcaccg aataccgcac ttatgatgaa ggcgagttga ttaatgaaac
     3061 gaatggccgg atagatagca cagttcataa attgagcacg tttaaaagcg ttgtattgct
     3121 ttttatttac ttcgtgtata gcagcttctg catgagcttg ctgattgaat gttttgacca
     3181 ctagattacc agctaagatt tcttccattc gattatttaa aacacccagt tcatactgat
     3241 tctgttcaga aatctgtttg tttttgttcg caatcttagt tgtaacaatt gaactgccaa
     3301 cgatcagcaa taagacgagc aaagtaagct tgctttcgat ataaaacagc atcagaccag
     3361 cagcagctat tgttacgaca gaagtaaaaa attgattgat acctgtcaat aacacttgag
     3421 aaagttgatt gagaccggta gttgtccgac taatgatgtc acctacttga tgattgtcaa
     3481 aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc ttttcgcacc ctcaatgtga
     3541 tcttttcgct taacgctgcc attgtccgtt cctgaataaa tgaagtgatg ctgcttaaga
     3601 ctgaacagcc gatcagcaat aagacaggga acaacaatgc ttgtttgata tcactgaaag
     3661 aaagctgtcc aatacctgtt tttcttattt ctgataagag atcatcgatc ccgattccca
     3721 tgatcaaggg catggctacg actaataggt tcccgaataa actgcataga aacaatccgt
     3781 aaaaatattt atgttcaggt ttgatcattt tccaaaaacg tatcaaagca cgcgtgttca
     3841 acgttttttt cttttccatc gtatcccttc tttctatatt ccttgtgatt ttgcaaactc
     3901 ctgataagtt tggcaattag acagtagttc ttcatgagta ccttgtccaa cgatttttcc
     3961 ttcatctaaa acaatgatgt tatctgcttg ttggattgta ctgaggcgtt gagcaacaat
     4021 cacgagagtt ttctccgaca tgtgtttatg aagggctgct cttaatgctg catctgtttt
     4081 atagtctaaa gcggagaaac tatcatcgaa tacatagatc tctgctggtt tgatcaacgc
     4141 tcgagcaata catatccgct gtttctggcc ccctgaataa ttagaaccgc cttgagcaac
     4201 aaagctgtcc aatccgtcag gaagggtgtc cactagttct tctaattgag cgatcttgat
     4261 cgctttagac aactcttctg ttgaggcata ttcatttccc atctgcaagt tgcttaaaat
     4321 tgtcccgctg aataagaaag ccttctgagg gacataactg atgtgggagc ggatctgttc
     4381 ttgagtcatt tgagtaagag gaataccacc aagggttact tttcctatcg tagggtcact
     4441 aagtctcaag agaagtttcg ccaaactact ttttccagat ccggtcccgc caacgatcgc
     4501 tgttgttttt cctttaggta ttgtgaaatc aatatcttct agaactggat cagagatttc
     4561 agtatattta aatgtgacat gttcaaaagc agctgcattt gtctgatcaa aggttggttc
     4621 agctactgtt ttgatcgtct cttcctcaat ttcgatctct gcttgcagta tttcttctat
     4681 ccgtcgtaat gaggcaaacg atttagggat aatgaccaat accatagctg cgatcatcaa
     4741 gtaagcaagt gtcagcatag agtattcgac tacagcagtc acgacaccga tttgtaatgc
     4801 tccttctgct acaagatttc cgccaaacca caaaatagaa gcataaacga ttcccatgag
     4861 caaaaaagca attggagtga cccaagcaaa tattcggttc actttgatca tttgattagc
     4921 atagtcagca aagctcttat tcgttcgctt ttcttcatat gattgattat caaaggcacg
     4981 gatcatatta atcccagtaa agaattctct caatgtgatg gtgatccgat ccatttttgg
     5041 ctggatactc aaagataaag gtgcccctcg tttcatcagt cgataaacaa tccatacata
     5101 aaaaacaaca gagatcaatg taacccatgt tagtaataga gaataacgaa acatcatgag
     5161 aatggtgaaa acgcaaataa ccggtgctgg taagatcatt tggcaaaata aaacaatcat
     5221 ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa agagatccag aactaaaacg
     5281 actgatatcc ttcattgaca gctgttggaa tttacggaaa tatttctccc gcgtttggaa
     5341 tccaaaccta gcggaagcca atgctgaaaa gtaactagag gcaactgcag cgattgttcc
     5401 tagaaacgct acaagcagca tttcaactcc aatttttttt ataactgagg cttcgccact
     5461 tgctatacct gtgtcgatca gctttgctac taattttggt acacctaatg taccggcgat
     5521 ttctaaacag atcatcacaa aggtaaggag agcgagtcct ttgtttttct gtaagaatgt
     5581 ttttaagata gacatattat tcctcctttt ttcctgatac ttttcgattc tataccttca
     5641 agtaacttga aggtcaatag aaaaaagaat aaactgaaaa agaggtgaga aaaaatgaaa
     5701 aaaatgtata cgattggcca agtatcggaa ctatttgatt tgccaaaatc aacgatccgc
     5761 tattgggatg aacaaggatt aatccgttca agtagacaag aagagaatga ttatcgattg
     5821 tttgatatcg atgatatttt tatgctttat gatatcgttt tttatagaaa attagatata
     5881 ccgatcaaac agatgaagaa tttatatgga aaaacattga cggagttata tgagacactt
     5941 gatgaaacag aaagaagaat ccataaagaa ctggtggtga tgaaacaaaa acaaaaagag
     6001 ataagggagc aaaaaaaaca gttgaaattg atgatcgata caaacgagga agaatttcct
     6061 gtagaagaga tcccttttga ctgtatgatc tcaacggagt ttgaagatat tgtggaaatc
     6121 aaaaaatttc tacctaacta ttcaagcttt gggatgatga gtatgccagc ttccagttca
     6181 cagacgatgt atggattttt tatcgaccct agtgaagtac atttatttac tcaagacgtg
     6241 atttgggaga aaaaagatac ggctgtttat cgtcgattct tattgaaatc cgagatgaac
     6301 catgcggaac gtaataatat tttagaaatc agagaaagaa tgtatgaaaa aggatggaaa
     6361 acaggagaag tgatcggcca gtatctattg acgaatacgg atgaaaacaa tatcagaact
     6421 gaatattatc atgcatggat agaaatgaaa aaataggaaa tatgtgttta agataactcg
     6481 aaaatgatgg ttgttttagt tataaagaaa aattttctgg ttcgattacg ggtttaatgt
     6541 gggggattat tagtcgtgga tagatagaat tttagttttt tagaggaaat at
//
LOCUS       DAZKOM010000127         6387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000127 DAZKOM010000000
VERSION     DAZKOM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.131"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8NF_002623"
     CDS             complement(99..1229)
                     /locus_tag="ACG8NF_002623"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0473031.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8NF_002624"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8NF_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0473032.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8NF_002625"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8NF_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0473033.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8NF_002626"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8NF_002626"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0473034.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8NF_002627"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8NF_002627"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473035.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8NF_002628"
     CDS             4880..5692
                     /locus_tag="ACG8NF_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473036.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8NF_002629"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8NF_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473037.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1916 a         1306 c         1006 g         2159 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataata
//
LOCUS       DAZKOM010000128         6295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000128 DAZKOM010000000
VERSION     DAZKOM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.132"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..263)
                     /gene="rpmB"
                     /locus_tag="ACG8NF_002630"
     CDS             complement(75..263)
                     /gene="rpmB"
                     /locus_tag="ACG8NF_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0473038.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            533..895
                     /locus_tag="ACG8NF_002631"
     CDS             533..895
                     /locus_tag="ACG8NF_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0473039.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            947..2629
                     /locus_tag="ACG8NF_002632"
     CDS             947..2629
                     /locus_tag="ACG8NF_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0473040.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2746..4782
                     /gene="recG"
                     /locus_tag="ACG8NF_002633"
     CDS             2746..4782
                     /gene="recG"
                     /locus_tag="ACG8NF_002633"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0473041.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4831..5832
                     /gene="plsX"
                     /locus_tag="ACG8NF_002634"
     CDS             4831..5832
                     /gene="plsX"
                     /locus_tag="ACG8NF_002634"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0473042.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5954..6196
                     /gene="acpP"
                     /locus_tag="ACG8NF_002635"
     CDS             5954..6196
                     /gene="acpP"
                     /locus_tag="ACG8NF_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0473043.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2094 a         1097 c         1428 g         1676 t
ORIGIN      
        1 caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc
       61 caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc
      121 caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca
      181 gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct
      241 gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga
      301 ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt
      361 catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa
      421 ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata
      481 aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt
      541 aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt
      601 tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa
      661 agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca
      721 agaagataat ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc
      781 tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt
      841 aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg
      901 agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga
      961 aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa
     1021 tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa
     1081 tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt
     1141 aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg
     1201 cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt
     1261 aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt
     1321 aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga
     1381 aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc
     1441 aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt
     1501 agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg
     1561 agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa
     1621 tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta
     1681 ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta
     1741 cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga
     1801 cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca
     1861 aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat
     1921 tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta
     1981 tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc
     2041 taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc
     2101 gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat
     2161 ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac
     2221 gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa
     2281 caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat
     2341 cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga
     2401 tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca
     2461 aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga
     2521 acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat
     2581 ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca
     2641 gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga
     2701 tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat
     2761 gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg
     2821 ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag
     2881 gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc
     2941 tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg
     3001 caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc
     3061 attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac
     3121 gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac
     3181 aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc
     3241 acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga
     3301 gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa
     3361 cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa
     3421 gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa
     3481 tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc
     3541 tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc
     3601 ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt
     3661 gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg
     3721 tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga
     3781 cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca
     3841 ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac
     3901 cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta
     3961 tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat
     4021 gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga
     4081 acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa
     4141 atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg
     4201 gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat
     4261 ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg
     4321 cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc
     4381 atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt
     4441 gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta
     4501 ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa
     4561 gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa
     4621 tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct
     4681 gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa
     4741 agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt
     4801 atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga
     4861 gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa
     4921 gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa
     4981 aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca
     5041 attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa
     5101 gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc
     5161 gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg
     5221 aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa
     5281 catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga
     5341 cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg
     5401 aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa
     5461 gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac
     5521 gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct
     5581 attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt
     5641 tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa
     5701 gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc
     5761 caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa
     5821 agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat
     5881 tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt
     5941 ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa
     6001 tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa
     6061 agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga
     6121 aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag
     6181 cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt
     6241 ttatccttct ttttttaatc tttttgacac gtaaaagtca tttttctgat aattc
//
LOCUS       DAZKOM010000129         6181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000129 DAZKOM010000000
VERSION     DAZKOM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.133"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8NF_002636"
     CDS             127..579
                     /locus_tag="ACG8NF_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473044.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8NF_002637"
     CDS             593..1282
                     /locus_tag="ACG8NF_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473045.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8NF_002638"
     CDS             1407..3758
                     /locus_tag="ACG8NF_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0473046.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8NF_002639"
     CDS             3838..4233
                     /locus_tag="ACG8NF_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473047.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8NF_002640"
     CDS             4558..5175
                     /locus_tag="ACG8NF_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473048.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8NF_002641"
     CDS             5216..5515
                     /locus_tag="ACG8NF_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0473049.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8NF_002642"
     CDS             5549..5716
                     /locus_tag="ACG8NF_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0473050.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..>6181)
                     /locus_tag="ACG8NF_002643"
     CDS             complement(5761..>6181)
                     /locus_tag="ACG8NF_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473051.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2129 a          850 c         1232 g         1970 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 t
//
LOCUS       DAZKOM010000130         6143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000130 DAZKOM010000000
VERSION     DAZKOM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.134"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..722
                     /locus_tag="ACG8NF_002644"
     CDS             <1..722
                     /locus_tag="ACG8NF_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0473052.1"
                     /translation="FLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLP
                     VLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVG
                     YSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEI
                     FLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQY
                     IGKKVKRPISADSVLLEEDVE"
     gene            719..1867
                     /gene="neuC"
                     /locus_tag="ACG8NF_002645"
     CDS             719..1867
                     /gene="neuC"
                     /locus_tag="ACG8NF_002645"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0473053.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1840..2517
                     /locus_tag="ACG8NF_002646"
     CDS             1840..2517
                     /locus_tag="ACG8NF_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0473054.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2468..3649
                     /locus_tag="ACG8NF_002647"
     CDS             2468..3649
                     /locus_tag="ACG8NF_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0473055.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            3662..4639
                     /locus_tag="ACG8NF_002648"
     CDS             3662..4639
                     /locus_tag="ACG8NF_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0473056.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            4639..5547
                     /locus_tag="ACG8NF_002649"
     CDS             4639..5547
                     /locus_tag="ACG8NF_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFQ0473057.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            5548..>6143
                     /locus_tag="ACG8NF_002650"
     CDS             5548..>6143
                     /locus_tag="ACG8NF_002650"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0473058.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNY"
BASE COUNT         2272 a          760 c         1162 g         1949 t
ORIGIN      
        1 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
       61 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
      121 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
      181 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
      241 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
      301 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
      361 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
      421 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
      481 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
      541 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
      601 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
      661 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
      721 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
      781 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
      841 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
      901 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
      961 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     1021 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     1081 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     1141 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     1201 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
     1261 taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa
     1321 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
     1381 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
     1441 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
     1501 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
     1561 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
     1621 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
     1681 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
     1741 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
     1801 aacattgaat caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc
     1861 ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc
     1921 attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa
     1981 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
     2041 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
     2101 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
     2161 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
     2221 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
     2281 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
     2341 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
     2401 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
     2461 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
     2521 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
     2581 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
     2641 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
     2701 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
     2761 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
     2821 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
     2881 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
     2941 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
     3001 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
     3061 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     3121 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     3181 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     3241 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     3301 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     3361 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     3421 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     3481 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     3541 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     3601 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     3661 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     3721 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     3781 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
     3841 tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga
     3901 ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga
     3961 tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt
     4021 gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta
     4081 tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt
     4141 ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg
     4201 gattatagat acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa
     4261 tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt
     4321 tggaacttta agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg
     4381 tgaaaagatt ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga
     4441 aacttctagt atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc
     4501 acatactatt attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga
     4561 aaagaaaatg gaaccccaat atacttttga aaaagataag tatattatta acttaattaa
     4621 cgacattgag gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag
     4681 agacatctaa aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca
     4741 gcttatagta gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac
     4801 gatttggata cacattatga tatagctttt attacaaatc cgacttttaa gcatctggaa
     4861 acactaaatg taattgttga aaaagttgat tacatatttt tagaaaaacc tgctttttct
     4921 gagagtattg atcttactca atataattat gattacaaaa atatttatgt tgctgctcca
     4981 ctgaggtata aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat
     5041 tctgtaagag ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga
     5101 aataattatt ctgcctttaa atctatgggg ggaggagtag aattagactg tatacatgaa
     5161 ttggattata ttataaacct atttggtttc cctaaatcag taaaaaaaca gtttggtaaa
     5221 tattcgaatc ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat
     5281 aaaattatag aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg
     5341 tttactttag atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa
     5401 aacattgtta aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat
     5461 ttctttgaaa acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa
     5521 gtattgaaaa tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct
     5581 gggtctaagg ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat
     5641 ttcgctttag caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact
     5701 tgtttgaata cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg
     5761 aaaattgtag aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata
     5821 cgtgattcat tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta
     5881 gatttgactt cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta
     5941 aaaaatttag ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt
     6001 aatatggtaa aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct
     6061 aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa
     6121 tttctaaaaa aaagtaatta tat
//
LOCUS       DAZKOM010000131         6132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000131 DAZKOM010000000
VERSION     DAZKOM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.135"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..955
                     /locus_tag="ACG8NF_002651"
     CDS             110..955
                     /locus_tag="ACG8NF_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0473059.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1236..2456
                     /locus_tag="ACG8NF_002652"
     CDS             1236..2456
                     /locus_tag="ACG8NF_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0473060.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2453..5173
                     /locus_tag="ACG8NF_002653"
     CDS             2453..5173
                     /locus_tag="ACG8NF_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0473061.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5157..6101
                     /locus_tag="ACG8NF_002654"
     CDS             5157..6101
                     /locus_tag="ACG8NF_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0473062.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2293 a          958 c         1196 g         1685 t
ORIGIN      
        1 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
       61 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      121 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      181 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      241 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      301 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      361 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      421 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      481 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      541 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      601 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      661 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      721 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      781 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
      841 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
      901 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
      961 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1021 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1081 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1141 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1201 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1261 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1321 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1381 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1441 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1501 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1561 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1621 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1681 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1741 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1801 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     1861 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     1921 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     1981 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2041 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2101 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2161 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2221 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2281 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2341 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2401 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2461 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2521 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2581 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2641 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2701 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2761 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     2821 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     2881 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     2941 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3001 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3061 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3121 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3181 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3241 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3301 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3361 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3421 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3481 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3541 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3601 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3661 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3721 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3781 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     3841 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     3901 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     3961 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4021 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4081 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4141 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4201 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4261 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4321 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4381 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4441 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4501 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4561 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4621 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4681 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4741 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4801 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     4861 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     4921 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     4981 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5041 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5101 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5161 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5221 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5281 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5341 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5401 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5461 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5521 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5581 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5641 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5701 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5761 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     5821 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     5881 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     5941 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6001 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6061 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6121 ttcaaaacaa aa
//
LOCUS       DAZKOM010000132         6049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000132 DAZKOM010000000
VERSION     DAZKOM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.136"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8NF_002655"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8NF_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0473063.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACG8NF_002656"
     CDS             complement(2944..3378)
                     /locus_tag="ACG8NF_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473064.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="ACG8NF_002657"
     CDS             complement(3395..4600)
                     /locus_tag="ACG8NF_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0473065.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="ACG8NF_002658"
     CDS             5294..5680
                     /locus_tag="ACG8NF_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0473066.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="ACG8NF_002659"
     CDS             5774..5965
                     /locus_tag="ACG8NF_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473067.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         1974 a         1068 c          831 g         2176 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataatta
//
LOCUS       DAZKOM010000133         5960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000133 DAZKOM010000000
VERSION     DAZKOM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.137"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..684)
                     /locus_tag="ACG8NF_002660"
     CDS             complement(37..684)
                     /locus_tag="ACG8NF_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0473068.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="ACG8NF_002661"
     CDS             complement(692..1378)
                     /locus_tag="ACG8NF_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0473069.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="ACG8NF_002662"
     CDS             complement(1384..2457)
                     /locus_tag="ACG8NF_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0473070.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="ACG8NF_002663"
     CDS             2679..3353
                     /locus_tag="ACG8NF_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0473071.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="ACG8NF_002664"
     CDS             3358..4698
                     /locus_tag="ACG8NF_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0473072.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="ACG8NF_002665"
     CDS             complement(4847..5833)
                     /locus_tag="ACG8NF_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0473073.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1805 a         1129 c         1049 g         1977 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     5881 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
     5941 aaacaattta attgaaattt
//
LOCUS       DAZKOM010000134         5732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000134 DAZKOM010000000
VERSION     DAZKOM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.138"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACG8NF_002666"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACG8NF_002666"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0473074.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1884..3038)
                     /locus_tag="ACG8NF_002667"
     CDS             complement(1884..3038)
                     /locus_tag="ACG8NF_002667"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0473075.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            3223..5664
                     /locus_tag="ACG8NF_002668"
     CDS             3223..5664
                     /locus_tag="ACG8NF_002668"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0473076.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1888 a         1072 c         1287 g         1485 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagttg
     1861 gctttcccct ctactcgatt ttattatatt ttgtagtaac gaatatcatt ttcgatcttt
     1921 gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc
     1981 tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct
     2041 tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc tgtaaaaaat
     2101 tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg ctgtagtgat
     2161 ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta aagattaccg
     2221 attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc ctgattttct
     2281 tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt atacggaata
     2341 tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt atgtcgattc
     2401 caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc aacaacagga
     2461 aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg
     2521 tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc accgacacct
     2581 tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc tatgactagt
     2641 acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca
     2701 ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg
     2761 tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc gacgatgacc
     2821 atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt tgctcctagt
     2881 gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat ccctatatgg
     2941 tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa atataaatta
     3001 ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct ttacttatga
     3061 taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa aaaagagaat
     3121 atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga gctatttgtt
     3181 tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga agtcgttatg
     3241 atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc atttactgca
     3301 gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact gaaaaaagat
     3361 aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc
     3421 agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac gatcaatgaa
     3481 gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca attaggtgaa
     3541 gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat gctgaaacag
     3601 tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa cgacggattg
     3661 actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc aacacaattt
     3721 tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa agcagcacat
     3781 gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc tgatgaagac
     3841 gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact gggcacgtta
     3901 cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc gttgaatgac
     3961 ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa tctgccttat
     4021 ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat gggaatcgct
     4081 ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc aacaatcgat
     4141 ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca agaactgcag
     4201 ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca tccaattggt
     4261 gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga acagaagaga
     4321 tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt gttagaagcg
     4381 aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct
     4441 gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc gttgattttt
     4501 caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca agtgagtgaa
     4561 gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa aaaatggatt
     4621 ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt
     4681 ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga
     4741 aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga gctgattctt
     4801 tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca agatatctct
     4861 actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt agtggatgta
     4921 aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc aaacaagtta
     4981 cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt tgctacagaa
     5041 tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa caaagaaatc
     5101 ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct tgacccttac
     5161 cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt ggcagctaca
     5221 ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag aaatggatta
     5281 taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa actaacggtg
     5341 cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc aaaagcatct
     5401 ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc tgcagtaggg
     5461 ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca aaaaggccac
     5521 atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga ggaaatcgag
     5581 caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc tatacaaata
     5641 ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa gcaagattta
     5701 cagttatttt atttactgca gatcttgctt tt
//
LOCUS       DAZKOM010000135         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000135 DAZKOM010000000
VERSION     DAZKOM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.140"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8NF_002669"
     CDS             complement(488..1297)
                     /locus_tag="ACG8NF_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0473077.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8NF_002670"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8NF_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0473078.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8NF_002671"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8NF_002671"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0473079.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8NF_002672"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8NF_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0473080.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8NF_002673"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8NF_002673"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0473081.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8NF_002674"
     CDS             4179..4913
                     /locus_tag="ACG8NF_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0473082.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8NF_002675"
     CDS             4897..5607
                     /locus_tag="ACG8NF_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0473083.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKOM010000136         5663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000136 DAZKOM010000000
VERSION     DAZKOM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.141"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8NF_002676"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8NF_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8NF_002677"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8NF_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="ACG8NF_002678"
     CDS             2092..3132
                     /locus_tag="ACG8NF_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0473084.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /gene="traE"
                     /locus_tag="ACG8NF_002679"
     CDS             3956..4288
                     /gene="traE"
                     /locus_tag="ACG8NF_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0473085.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="ACG8NF_002680"
     CDS             4907..5248
                     /locus_tag="ACG8NF_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0473086.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(5485..>5663)
                     /locus_tag="ACG8NF_002681"
     CDS             complement(5485..>5663)
                     /locus_tag="ACG8NF_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0473087.1"
                     /translation="VKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLR
                     KHQLYTPPRSVIEK"
BASE COUNT         2031 a          775 c          967 g         1890 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 tttatttagt ctaatggtga taagtcaaga tgaaatttaa gattttcttt attgagtaat
     5401 atgttttgaa gcgcacgaaa ctagacccga tcaaaatcaa atttttttcg ggttgattca
     5461 aggatttgcg tcaataacgg attgttattt ctctatcaca ctccttggtg gcgtatagag
     5521 ttggtgttta cgtagtagcg tatccaccag acgcgtaaat tttcttgcgg ttaggacgag
     5581 tgcacgtttg tgttgatgct ttggaacctc cttatattta ctttgataaa aagctttgta
     5641 ttcaggaaga taatttttta cag
//
LOCUS       DAZKOM010000137         5554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000137 DAZKOM010000000
VERSION     DAZKOM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.142"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..682)
                     /locus_tag="ACG8NF_002682"
     CDS             complement(<1..682)
                     /locus_tag="ACG8NF_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFQ0473088.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
     gene            complement(654..1853)
                     /locus_tag="ACG8NF_002683"
     CDS             complement(654..1853)
                     /locus_tag="ACG8NF_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0473089.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1983..3359)
                     /locus_tag="ACG8NF_002684"
     CDS             complement(1983..3359)
                     /locus_tag="ACG8NF_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0473090.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3359..4015)
                     /locus_tag="ACG8NF_002685"
     CDS             complement(3359..4015)
                     /locus_tag="ACG8NF_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0473091.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(4025..5302)
                     /locus_tag="ACG8NF_002686"
     CDS             complement(4025..5302)
                     /locus_tag="ACG8NF_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0473092.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(5321..5482)
                     /locus_tag="ACG8NF_002687"
     CDS             complement(5321..5482)
                     /locus_tag="ACG8NF_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0473093.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         1727 a         1026 c          717 g         2084 t
ORIGIN      
        1 ctcgttcgcc tttgacgtag tagccgcttt ccggatcagc tttacttatt tttctttctt
       61 tcagcttcac ttccttttct aagtccaaag taaaagggcg ttttccagcc tctattcgtt
      121 gacgattgat ctcatcttct aattcatcct gataggcttt cgcttcatca tgggtcattt
      181 ctttcgtaaa cttatgttta ttggcgttag ctttgatgtg ggtagaatcc agatacaaat
      241 tatcctctgt tacaaaacct gctttaactg cttgttctaa aatataggca aaaatatcct
      301 caaacaccgt cgtttctcta aatcggcgaa cataattctt tccaaaagtt gagaaatggg
      361 ggattttctc ctcgaaggag tagcctaaaa accagcggta agcgacattg gtatcgactt
      421 ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac accaatttga
      481 tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct tcgacgattg
      541 gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga tcttgaggaa
      601 ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg tttttagaca
      661 tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa aagactgtag
      721 acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga gaacactccc
      781 ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt agtgtacatg
      841 atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa gtttcgtttg
      901 catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc gaaggcagcg
      961 tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca gacttcctta
     1021 taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat acagcgctgc
     1081 acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac accgtccatc
     1141 gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac ttgcatccag
     1201 cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt tcctttaaga
     1261 tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc gcgtaaagtg
     1321 acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg agcttgccat
     1381 tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg ggaaatatca
     1441 aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc tcgtgcatac
     1501 atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg aatcaactca
     1561 ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc aaagctcgtt
     1621 ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg ctctttagaa
     1681 tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc aaataaggga
     1741 ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg ataagtattc
     1801 aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc catcgtgtaa
     1861 tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc gcctaggatt
     1921 gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa gcaccgaaaa
     1981 ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt gaatgaagaa
     2041 ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat catgaattta
     2101 ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc ttctgcccca
     2161 ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc accagtaacc
     2221 ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa ataggaacca
     2281 acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa aatttctaat
     2341 tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt catccataag
     2401 aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa taatttattt
     2461 gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga tgaactttta
     2521 atcaaattat attctctcca atttatatct aatttcccaa gatcttttat tacactatca
     2581 agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt atcttcggta
     2641 ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc gtatagttct
     2701 tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat ttctacttct
     2761 actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt tatttttatc
     2821 ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa gattttattt
     2881 ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga tacaaatttt
     2941 gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa attctttgct
     3001 ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata atcaactaaa
     3061 tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg agatatcttt
     3121 ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt tacttgtcta
     3181 tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga agctctgtcc
     3241 gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa aataactaat
     3301 atgataatta atgattttaa actttttcta gttacataag caaatgcatt ttttatcatt
     3361 attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt agttctaaaa
     3421 tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca catttatttc
     3481 tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca ctgtctagat
     3541 taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct ctagcaatag
     3601 ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt tgttttttat
     3661 ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat ctaatatttt
     3721 caattggtga taaataatct attaaattat agttttgaaa tactaaagat atattatttt
     3781 ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat atttttcctg
     3841 tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct gttcctgact
     3901 ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct gacaaaactt
     3961 tttctttaga atttgcataa ctatatgcta cattctttat ttctaatata tccattattt
     4021 tctcctaact tatttttgat aagatttctt taggcttctt gattaacatt aatgaagaag
     4081 caaatacaac tgataaaaca ataatactta ttaatattaa ataactttgt ccaagtgttg
     4141 ttatatttga cataaaacta ctattattta ttaaacttcc accggaaatc attgaatcct
     4201 ctgagttaat gaatccatct acaattactt ttagtaatac atttcctaaa aataaggaag
     4261 atattatact tggtatcgat atgaatatta actcgaatat aaattgcatt ataatttgta
     4321 tcttagatgt tccaatagat aagaatatac ctatttcata aattctttcc cttaaccata
     4381 gaatcaagat taatgaaaga acaaccatcc cacctaacat aatagaataa gtcattactt
     4441 ttattatata ttttattcca ctcactgact ctaaagactc ttcaaacgca ttagaatctt
     4501 tttcaacaaa atactttgac tcatcaattt taagctcttt caatttgttt aaggctaagt
     4561 ctgtagattc tgcactacca gaatacatta aaattttatt tgcaatttca ttattttctg
     4621 aattatttaa tatctcttgg cttgttgaat aatctacaaa caccatattt tcactaaaat
     4681 cagaagataa tcctgtatat gtttcctgtt ttttaccaga aaagatccct ataattttaa
     4741 atttatgact ttttattttt ccactttttt caatatctag taattcaaga tcaacttcat
     4801 cacctaattt tagattgttt tgtttagcaa attcttcatg aacaataatt gaattcttat
     4861 cattttcttc tatattttta ccttctttaa ttgtaaatac tccactacta aataaaatat
     4921 ttcttttagt attatttgta gcttcgagtg aaacaacatt cttaaattcg tcagataaat
     4981 cttctctatt tattctttgt tctccgctaa ctactttagc atcttttagt tttgctaatc
     5041 catcatattg aattattttt tcttcaattt cttttaattt ttcaatatct ttaaattgat
     5101 taacattaaa atatttacca tctttttttg ttattgatat tgaagaatta gaactttcat
     5161 ataaagcctt ttctatttca ttgcttgatt tcattattgt taaacaagaa tacaagcaag
     5221 aaagaactat agttaatatg ataaaaatta ttaaagttct atttttcttt cttgtaatat
     5281 atgctatagc attttttatc actttcctaa ctactcctct ctattttctt actccataac
     5341 taattgcatc cactggacaa gcatttttac atcgtccaca tctgatacac tccagatgat
     5401 tgcaattctc aactgaatct ttattcattt ggcaaacttt tttacatttt ccacaattaa
     5461 tacacttatc cttatcctga atcggctaca ctagactaga cagaaaaaat aaggtgtgta
     5521 gaataataaa aaacacactg gaggattttt catg
//
LOCUS       DAZKOM010000138         5509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000138 DAZKOM010000000
VERSION     DAZKOM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.143"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..846
                     /gene="purR"
                     /locus_tag="ACG8NF_002688"
     CDS             28..846
                     /gene="purR"
                     /locus_tag="ACG8NF_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0473094.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1349..2722
                     /gene="glmU"
                     /locus_tag="ACG8NF_002689"
     CDS             1349..2722
                     /gene="glmU"
                     /locus_tag="ACG8NF_002689"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0473095.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2816..3787
                     /locus_tag="ACG8NF_002690"
     CDS             2816..3787
                     /locus_tag="ACG8NF_002690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0473096.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3793..3895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3961..4530
                     /gene="thiT"
                     /locus_tag="ACG8NF_002691"
     CDS             3961..4530
                     /gene="thiT"
                     /locus_tag="ACG8NF_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0473097.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4611..5384)
                     /locus_tag="ACG8NF_002692"
     CDS             complement(4611..5384)
                     /locus_tag="ACG8NF_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473098.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1801 a          978 c         1160 g         1570 t
ORIGIN      
        1 aaaatttctt aaaaggagtt ggaataggtg aaagtacgtc gcagtgaacg tctagttgac
       61 atgacacagt atttattgaa tcacccgcat gaattgattt ccttaacaag ctttgccgaa
      121 cgttatgaat ctgctaaatc ttctatcagt gaagatttgg gaatcatcaa gaaaacattt
      181 aaagaacgag ggtatggaac cttggaaaca atcccgggtg ctgcaggtgg tgtattgttc
      241 atccctgaaa tttcatatga agaggcgaaa aaatatattg aatctctagc agaacgtcta
      301 tcagaacaag accgtctgct tccaggagga tatgtttatc tctctgattt gttaggagaa
      361 cctgaactgc tgcgtcaagt tggaagaatc attgcttcta aataccttgg tgaaaaaatc
      421 gatgctgtaa tgactgtagc cacaaaaggc gtacctattg cccaagctgt gtcctattat
      481 ctgaatgcgc cattcgttat tgtacgccgg gactcaaaaa taacagaagg atcaacagta
      541 agtgtcaact atgtgtctgg atcttctgaa agaattgaaa agatggaact gtctaagaga
      601 agtttgaaac gtggttcacg tgtccttgta gtggatgatt tcatgaaagg cggcggaaca
      661 gtcaacggaa tgaagagctt gattgaagaa ttcgaatcag aacttgttgg catcactgta
      721 tttgccgaat caaaattcaa cggtcatcgc gccatcgatg attatacgtc actgctttat
      781 gtcaaagatg tagatactca aacgaaaaat attacggttg tgccaggaaa ttatttttca
      841 gaataagagg ttgtgaaaaa gccgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat
      901 ggctttccac attttttcat aaatttggct taataccgag aaactggctt ttgaacaccg
      961 tttatctaga ggttgtgaaa tcagcgtcct gtcctgagta ttagctcaat aaacggaaaa
     1021 attgctcctc atattttgcc gtttattgag ctaataccga aggtcagctg attgaaaaac
     1081 gtttaatgaa gaggttgtaa gcctaccgtt taactctaag tcacaagaaa cgatttcaaa
     1141 aaacagcttt tcatgtttta tgagtagtgt gacttgtgac cgaggagttg gcaggagagc
     1201 accggttacc aaatagatag gcccgttatg gtctatctat ttttttatcc agaaaacgct
     1261 gggataaaag aaaatcactt gttttgttga aaaaatgggt atactgggat aaactgaaat
     1321 cagcaaacta tatttaggag tgacgcagtt ggacgcacgt tatgcaatta ttttggcggc
     1381 agggaaaggt acccgcatga agtcaaaatt atataaagtt ttacatcccg tttctggtaa
     1441 acctatggta gaacatatca ttaatcgggt cagtgaaacg aaaccagatg aagtgattac
     1501 gatcgttgga cacggagcag aacaagttaa agcacaacta ggagaacgca gtaaatatgc
     1561 gttacaagca gaacaattgg gaacaggaca tgctgttttg caagctgctt ctttcttgga
     1621 aggaaaaaaa ggaacaacgt tagttatcag tggcgataca ccattattga ctactgaaac
     1681 gctgaacaat ctttttgaat atcaccaagg aaaaaatgca agtgcaacga tcttgacggc
     1741 acaagcagaa aacccaacag gatatggacg aatcatccgt gaccatattg ggatcgtaga
     1801 aaaaatcgtg gaacaaaaag atgcaacacc agaagaagct cttgtacaag aaatcaatac
     1861 aggaacttac tgttttgaca acgaagcttt gttcgatgcg ttaagcaaag ttggaacaaa
     1921 caatgctcaa ggtgaatact atctaacaga tatcatcgaa atcttaaaag aagaaggcca
     1981 tacagttgct gcttatcaaa cggatgattt tgaagaatca atgggagtca atgatcggat
     2041 cgcgttagct aaagcaaatg agatcatgag aaagcgaatt aaccagatgc atatggtcaa
     2101 tggcgtaagc tttgtcgatt cagcaacgac atacatcgat gcaggcgtag aaatcggtcc
     2161 agacacattg atcgaagcag gtgtacaaat ccaaggaaat acagtcattg gttctgattg
     2221 tgtcatcggg tcacattcaa aaatcgtaga cagtaggatt gaggatcacg tcgtcattga
     2281 aaactcagtc attgaaagca gccatgtcaa gaaacatgca gatgtgggac cttatgcaca
     2341 tttacgacca aaagctgaaa ttggcgaaaa cgtccatatc ggaaactttg tggaagtgaa
     2401 aaatgctcaa atcggcaaag gaactaaagt cggtcacttg acttatgttg gtgatgctac
     2461 gctgggagaa gaaatcaatg tcggatgtgg tgttgtcttc gtcaactatg atggaaagaa
     2521 caaacatcat acgactgtcg gcgaccacag ctttattggt tctagcacga atatcatcgg
     2581 acctgtcgag gtagctaaaa acagttcgat tgctgccggg tcaacaatta cagacaatat
     2641 tccagaatat gcgctggcaa ttgcgagagc aagacaagtc aataaagaag gatatgcaaa
     2701 aaaactccct tatctaaact aagacttttg caaaatcact tgatttattt aaaaaatatg
     2761 actactattt attaggtaga attctagagt tgtgaaaaag aaagtggagg tccccatgtc
     2821 aaaacattac tttgacccaa gattaaaaat ttttgcgtta aattccaatc gcccattagc
     2881 agagaaaatt gcagcagcag tcggcgtcga gttaggaaaa tcatctgtta ctcaatttag
     2941 tgatggagaa attcaagtaa acattgaaga aagtattcgt ggatcgcatg tctatgttat
     3001 tcaatcaacg agcagcccag tcaatgataa cttgatggag ttgctgatca tgattgacgc
     3061 actgaaacga gcaagtgcaa aaacaatcaa cgtagtcatg ccttattatg gctatgcgcg
     3121 tcaagaccgt aaagcacgtg cacgggaacc aatcacagcg aaattagtag caaacatgat
     3181 tcaaaaagct ggtgcaacaa gaatgctgac tttggacctt catgcagtcc aaatccaagg
     3241 tttctttgat attccagtcg atcatttgat gggtgcgcca cttattgcca attatttctt
     3301 agaaaaagga atcaaaggcg atgatgttgt cgttgtttca ccagaccatg gtggtgtcac
     3361 acgggcacgt aaattagcgg aatttttgaa atctccgatt gcgatcatag acaaacgccg
     3421 tccaaaagca aatgttgcag aagtaatgaa tatcattgga caagtcaaag gcaaaacttg
     3481 tgtactgatc gatgatatga tcgatacagc aggtacgatc acattggctg ccaatgcttt
     3541 gaaagaagct ggtgcaacta gtgtgtatgc ttcatgtaca catccagtat tatccggccc
     3601 tgctttacaa agaatagaag attcagctat cgagcgtcta gttgttacag actcgatcta
     3661 tcttccagaa gatcgtaaaa tcgacaaaat cgatgaagta agtgttggcg gactaatggg
     3721 agatgcaatc aaacgtatcc atgaaaacaa accagttagt ccattgtttg aaacaaaaaa
     3781 taaataataa ataaactcac aaaggggagt ccaattgggc tgagattgaa tctatttcta
     3841 aacccttcgt acctgtatcg gttatgcgag cgtaggaatt gtgaataaac agctgtttga
     3901 ctattttcga aagctgcttc ctcctttgtg acttttttat tcaaaggagg aagttttttt
     3961 atgggaaaaa atagagtttg ggtagaagga acgattgttg cagcgttagc gatggtgttg
     4021 tcgttgattc cgatccagat cggcagcagt ttttctattt cattagggca gattccattg
     4081 actctttttg cgttaagaag agggacaaag gcagggatgc tggcaggttt catttggggc
     4141 atcctacatt ttccgttggg acaagtttat tatttaagtg tcgttcaagt gttgattgag
     4201 tacccgattg cctatacttt tgcaggcgca gcagggctca tggcttcaaa tgtccaaaaa
     4261 ggctatgtag acgataatac gaaacaaatt agaaaaagca tcgtgtttgg tgcattgatt
     4321 ggcgcacttt ccagatattt ttggcatttt attgcaggag tggttttctg gggagcgtat
     4381 gcactatggg ggatgaatcc ttggctcttt tctttcgtaa tgaatggtgc cagcgggttg
     4441 agtacagcta ttgtaacgat catcgtttta ttagggatcc aacaaacgac tccttcgatt
     4501 tttgtgccag ataaacgaca ggagctgtaa ataaaaaaga agcaagaaac ttgagacaga
     4561 atcatgtcct agtttcttgt ttccttttta ttttttgata agaaatagat ctacttcaat
     4621 ggcagataag aaagatcggc atcttctacc aattgccagt gtcgatcggt gacagagtag
     4681 attaaagtag cgatgatttc ctcttctcga taccgggtgc cttttaattt ccaaatataa
     4741 tcttcaatca catagattgg gatacgatat ttaccatcga tcaaaccaac ttgatcaaaa
     4801 gaaaaaatga tcccttcctg catcaaatta cgcatttctt tcaataattc cacactgatt
     4861 ttttcaacag gtgtttcttt tttgaaaaaa gattttttta cattgatctt atctgacaag
     4921 atcgaactta atttactagc aaaaatccta taaaattcat ctaaatagcg ataataaatc
     4981 cgattcagct gatcattaag atcgaagtat acaaaattat tctgtagttt ataaaaaaac
     5041 ggcgaatgaa gatgtgtttt catatgacca aaatataata attctgaaat ttctaaaggt
     5101 gtcaattctt tcagcatcgt taaatcagta aagtcgatcc acttcaattc atcgaatcca
     5161 cgcctgttac gagaagagga aaagaaacgc tgaatatttt ctgtaccacg gattactttc
     5221 agccctgtat gggattcata ttctccagca gaagcagatg gatcaagcaa gagcaagttt
     5281 tgaggaaaat gcacgatact gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca
     5341 gcattacttg tggtatcaat atgtacataa actgactcag tcatcatgtt cctcctctta
     5401 tttcctctta atctctttta ttttacaaca tttgcattaa aaaagcttgt ttcttttatt
     5461 ttaaaattcc attacaaaat cacgttttca ttgaaaacac tctcatttt
//
LOCUS       DAZKOM010000139         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000139 DAZKOM010000000
VERSION     DAZKOM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.144"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8NF_002693"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8NF_002693"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG8NF_002694"
     CDS             complement(580..1353)
                     /locus_tag="ACG8NF_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0473099.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG8NF_002695"
     CDS             1497..1847
                     /locus_tag="ACG8NF_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0473100.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG8NF_002696"
     CDS             1828..2544
                     /locus_tag="ACG8NF_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0473101.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG8NF_002697"
     CDS             2544..3992
                     /locus_tag="ACG8NF_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0473102.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG8NF_002698"
     CDS             complement(4062..4571)
                     /locus_tag="ACG8NF_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473103.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG8NF_002699"
     CDS             complement(4561..5286)
                     /locus_tag="ACG8NF_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473104.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKOM010000140         5350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000140 DAZKOM010000000
VERSION     DAZKOM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.145"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..574)
                     /locus_tag="ACG8NF_002700"
     CDS             complement(32..574)
                     /locus_tag="ACG8NF_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0473105.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            709..1098
                     /locus_tag="ACG8NF_002701"
     CDS             709..1098
                     /locus_tag="ACG8NF_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473106.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1102..1866
                     /locus_tag="ACG8NF_002702"
     CDS             1102..1866
                     /locus_tag="ACG8NF_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0473107.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1995..2942
                     /locus_tag="ACG8NF_002703"
     CDS             1995..2942
                     /locus_tag="ACG8NF_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0473108.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3072..3812
                     /locus_tag="ACG8NF_002704"
     CDS             3072..3812
                     /locus_tag="ACG8NF_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473109.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4103..4771)
                     /locus_tag="ACG8NF_002705"
     CDS             complement(4103..4771)
                     /locus_tag="ACG8NF_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0473110.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4905..5192
                     /locus_tag="ACG8NF_002706"
     CDS             4905..5192
                     /locus_tag="ACG8NF_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473111.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT         1830 a          902 c         1034 g         1584 t
ORIGIN      
        1 ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt ctcacttttt
       61 tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa gcacgctctt
      121 cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa agtatttcga
      181 gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca taaccgtgtt
      241 ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa atcgttgtga
      301 ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg atacctactg
      361 cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct aaaacaaggg
      421 ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca atcagtcgat
      481 agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca aaattaatga
      541 ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc atttatataa
      601 caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca tacaaaaaaa
      661 tgataaaatg attataatag aattataaag ataggttagg tgattaaaat gaaaacggtc
      721 aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata cttaggtgtc
      781 tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc gctgacagat
      841 ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat tttcggtagc
      901 gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt aggagtggtg
      961 ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat attatttggt
     1021 acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc aggaaattta
     1081 atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt ccatcatatt
     1141 ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt tgtcatctat
     1201 tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa aattgcgcag
     1261 cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa acagccggtt
     1321 tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt tgaatttgac
     1381 acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc agttggcggg
     1441 gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca tatagtagcg
     1501 ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc
     1561 gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc atgggttaag
     1621 agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt cttttggcat
     1681 ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga aaaaaatcgg
     1741 cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg acatatggta
     1801 caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat aaaaaagtat
     1861 aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt ttattttggg
     1921 ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga caacatgtta
     1981 gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat gtttcaaaaa
     2041 tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg aaagagttag
     2101 ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa gcacttgtca
     2161 cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca actgagccct
     2221 attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat tattctctcc
     2281 aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc atcacaggtg
     2341 tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc ttgtttgggg
     2401 aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca gcaaaagcaa
     2461 cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc gaagtggacg
     2521 agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa gttgatcttc
     2581 ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat atgacagaaa
     2641 actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag
     2701 gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt ggtgtgattg
     2761 gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg atcaagcaag
     2821 atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag
     2881 gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag acaacgaatt
     2941 aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa aataatcagc
     3001 gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa
     3061 ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg
     3121 agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc
     3181 ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca
     3241 cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc
     3301 gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat
     3361 cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac
     3421 aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg
     3481 tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta
     3541 gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt
     3601 atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg
     3661 tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat
     3721 cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa
     3781 actctttggg agaaaatagc tattttaaat aagtgatata caaatttata ttcggctaca
     3841 gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa gtttatatcg
     3901 tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa gaaaataatg
     3961 gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt atttttttat
     4021 aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc tgtaaattaa
     4081 ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca agtggttcaa
     4141 atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt tccagatact
     4201 tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac agaaaatgtg
     4261 tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag aaaagacttt
     4321 tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct gttccgcctg
     4381 tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata cctgtcatca
     4441 aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc cataaatgta
     4501 aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa
     4561 gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga
     4621 atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct gccagcttag
     4681 atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa atgattccgc
     4741 acaattctgt aaataaattt aattttttca tttctagcct cttttctttt gaaaattttc
     4801 agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg aaaaaaaatt
     4861 atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga gcagcagact
     4921 tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt tttttggctc
     4981 aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat caatttatct
     5041 ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt tttttgatga
     5101 agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct ctttatggag
     5161 tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg
     5221 atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac agtataatat
     5281 tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta ttggctgttt
     5341 ctttttatta
//
LOCUS       DAZKOM010000141         5312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000141 DAZKOM010000000
VERSION     DAZKOM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.146"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8NF_002707"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8NF_002707"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0473112.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACG8NF_002708"
     CDS             complement(1298..2134)
                     /locus_tag="ACG8NF_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473113.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACG8NF_002709"
     CDS             complement(2318..3535)
                     /locus_tag="ACG8NF_002709"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0473114.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3845)
                     /locus_tag="ACG8NF_002710"
     CDS             complement(3684..3845)
                     /locus_tag="ACG8NF_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473115.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACG8NF_002711"
     tRNA            3918..4001
                     /locus_tag="ACG8NF_002711"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5312)
                     /locus_tag="ACG8NF_002712"
                     /pseudo
     CDS             complement(4241..5312)
                     /locus_tag="ACG8NF_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1083 c          838 g         1738 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaacactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DAZKOM010000142         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000142 DAZKOM010000000
VERSION     DAZKOM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.147"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8NF_002713"
     CDS             137..748
                     /locus_tag="ACG8NF_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0473116.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8NF_002714"
     CDS             821..1750
                     /locus_tag="ACG8NF_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0473117.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8NF_002715"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8NF_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG8NF_002716"
     CDS             complement(2693..3895)
                     /locus_tag="ACG8NF_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0473118.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG8NF_002717"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG8NF_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG8NF_002718"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG8NF_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0473119.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKOM010000143         5176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000143 DAZKOM010000000
VERSION     DAZKOM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.148"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG8NF_002719"
     CDS             complement(136..1098)
                     /locus_tag="ACG8NF_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0473120.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG8NF_002720"
     CDS             complement(1100..2539)
                     /locus_tag="ACG8NF_002720"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0473121.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG8NF_002721"
     CDS             2724..4670
                     /locus_tag="ACG8NF_002721"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0473122.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="ACG8NF_002722"
     CDS             4934..>5176
                     /locus_tag="ACG8NF_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0473123.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DAZKOM010000144         5088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000144 DAZKOM010000000
VERSION     DAZKOM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.149"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACG8NF_002723"
     CDS             complement(205..660)
                     /locus_tag="ACG8NF_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFQ0473124.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="ACG8NF_002724"
     CDS             complement(1269..1685)
                     /locus_tag="ACG8NF_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473125.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="ACG8NF_002725"
     CDS             complement(1687..1968)
                     /locus_tag="ACG8NF_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473126.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="ACG8NF_002726"
     CDS             complement(2415..3383)
                     /locus_tag="ACG8NF_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0473127.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4550..4705)
                     /locus_tag="ACG8NF_002727"
     CDS             complement(4550..4705)
                     /locus_tag="ACG8NF_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473128.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4724..>5088)
                     /locus_tag="ACG8NF_002728"
     CDS             complement(4724..>5088)
                     /locus_tag="ACG8NF_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFQ0473129.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1595 a          839 c          839 g         1815 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DAZKOM010000145         4913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000145 DAZKOM010000000
VERSION     DAZKOM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.150"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..670)
                     /locus_tag="ACG8NF_002729"
     CDS             complement(308..670)
                     /locus_tag="ACG8NF_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473130.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="ACG8NF_002730"
     CDS             complement(744..1124)
                     /locus_tag="ACG8NF_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0473131.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1206..2381
                     /locus_tag="ACG8NF_002731"
     CDS             1206..2381
                     /locus_tag="ACG8NF_002731"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0473132.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2458..3042)
                     /locus_tag="ACG8NF_002732"
     CDS             complement(2458..3042)
                     /locus_tag="ACG8NF_002732"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0473133.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="ACG8NF_002733"
     CDS             3243..4241
                     /locus_tag="ACG8NF_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0473134.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="ACG8NF_002734"
     CDS             4353..4862
                     /locus_tag="ACG8NF_002734"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0473135.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1562 a          917 c          853 g         1581 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt ttt
//
LOCUS       DAZKOM010000146         4757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000146 DAZKOM010000000
VERSION     DAZKOM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.152"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8NF_002735"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8NF_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0473136.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8NF_002736"
     CDS             724..1878
                     /locus_tag="ACG8NF_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0473137.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8NF_002737"
     CDS             2093..3061
                     /locus_tag="ACG8NF_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0473138.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8NF_002738"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8NF_002738"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0473139.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8NF_002739"
     CDS             4091..4699
                     /locus_tag="ACG8NF_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0473140.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAZKOM010000147         4747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000147 DAZKOM010000000
VERSION     DAZKOM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.153"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8NF_002740"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8NF_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0473141.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG8NF_002741"
     CDS             complement(1164..1874)
                     /locus_tag="ACG8NF_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0473142.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2802)
                     /locus_tag="ACG8NF_002742"
     CDS             complement(1942..2802)
                     /locus_tag="ACG8NF_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0473143.1"
                     /translation="MFVAIWGKWSTRRIKMKVLGLGDNVVDKYENLGIMYPGGNSLNF
                     AVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGD
                     RIFLGSNSGGVTREYPIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYD
                     FSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIG
                     YDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVD
                     GSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG8NF_002743"
     CDS             complement(2768..3736)
                     /locus_tag="ACG8NF_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0473144.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>4747
                     /locus_tag="ACG8NF_002744"
     CDS             4499..>4747
                     /locus_tag="ACG8NF_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0473145.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLE"
BASE COUNT         1460 a          980 c          721 g         1586 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggag
//
LOCUS       DAZKOM010000148         4702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000148 DAZKOM010000000
VERSION     DAZKOM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.154"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..266)
                     /locus_tag="ACG8NF_002745"
     CDS             complement(<1..266)
                     /locus_tag="ACG8NF_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473146.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQ"
     gene            834..2069
                     /locus_tag="ACG8NF_002746"
     CDS             834..2069
                     /locus_tag="ACG8NF_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0473147.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(3068..3328)
                     /locus_tag="ACG8NF_002747"
     CDS             complement(3068..3328)
                     /locus_tag="ACG8NF_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0473148.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(3355..4656)
                     /locus_tag="ACG8NF_002748"
     CDS             complement(3355..4656)
                     /locus_tag="ACG8NF_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0473149.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         1591 a          959 c          734 g         1418 t
ORIGIN      
        1 tcttggtatt ttgatacaga attcaatgat tgaatctcat ttattttttt cctgattttg
       61 tcttcttctg tttctactaa tatatcggag atctcgacat tcagcttttt gcaaagcgca
      121 cttaagatat tgatgcctgg cgacatattc ttgttttcga tattgctgac agttgcttgt
      181 tcacaaatgc cttctgctaa ttgtacttgt gttaacccct ttttctttcg gtattctttt
      241 agtaatgctc catctacttt aatcaaaaca atttccttcc ctttaaaaaa ttatttctcc
      301 tttatatcat acacaaaaaa aagttctaaa caaccaaaaa tgtttagaac agaataaaaa
      361 ttttaatttt aaaataatat tcaaattaaa caactggcgt atctgctcct tgatttcttt
      421 ttgtgttatc tgaataatta tcgtttacag ttataacaca tagtgaaaat tgtcatggcg
      481 agaaagtcta agaatatttt taaacataac tatttctttc ttacgtgtgt ataatgacga
      541 cagcacacct atcaattaat ataattattt tattttttta tgttctcatg tttattttta
      601 atgacacaac ttctcgtatt tatctggcac aacatataaa tcgatcaaaa aattgttgcg
      661 tgtttttttg aaaattggag tttcaacttg attacaaaat ttaaatttag aagttgtgaa
      721 aacagcgaga gacagtttaa tattcaaaat atacataggc tactcgaacg ctgtgttttt
      781 tagtatacta gtaagaaacg aagggataga ctcattccaa taaggaggat ttcatgaaga
      841 caattctttt tgctcctgca gtattcaatt tagctgagac aacacgaatg attgaggttg
      901 ccaaagctat gaaagaccgt tattactgtg tattctttgg cttttctaaa acttttgctc
      961 ctttgatcac agaaagtggc tttgagtatc atttactagc gccgatcctt acgaaaaaac
     1021 aagaagaaca aatcataaaa tttgaccagt tgcgtacact taaaaaccct tttaccaaag
     1081 aaatggttgc ctctcgtatc actagtgaac agagtctgat tgctaagttt gagcctcgac
     1141 tgattgtgac aggttcgaat gtgacaattt ttttatcggc ccgcaataaa aagattcctt
     1201 tagtatatgt taaaccctat gctttgagtg aacctttttt tcaatcagag catgcgctga
     1261 tgccccacga gttacgtttc ttcggccctt taagcaatcc cctttggcac tggacaaaaa
     1321 agcgtatagt aaatacccga tggatcccca atgccttcaa acacgctgca caaaaagaaa
     1381 tgcttccttt accaaaaaca agtttacaaa tgatggatgc tgatctaaat ctcatcacaa
     1441 cgcctaaact atttttggaa gaagaacagc tgccggaaaa ttatcgaatc gttggtccaa
     1501 tttttgctaa gctcagtact tccttgcctg aagaagtgct aacttttata cagcgaaagc
     1561 aacaagaaag aaaacgtatt atctatttcg caatgggtag ttcaggcaat ccgcgtatgt
     1621 tacgcaaaat tttgttcttc ttacaaaaac aaccggatct agcggttatt gcacctattg
     1681 actatctgct tgatgacatt caatctgact cagaagatct tttaataaca gaatatctgt
     1741 ctagtcatct tttgcatgaa ttcattgatt tttcttttat ccacggcggg gaagggaccg
     1801 tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt ggggatgcaa tttgagcaaa
     1861 gctgtaatat tgactattgt gtaaaatacg gaaacgcaat cgccctcaca aaaccagtga
     1921 ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc acccaagatt cgtcagcagg
     1981 cacgcacgct gcaagccgtt tttccaataa atggtccaca acaagcaatt caagaaatcg
     2041 aaaccttcct gagcaaccac ccaatatagt ttcaagtaga aaaagctttt ttaatagaga
     2101 ctcatcagta atttcgctat tcgagaccca gacagtgaaa atacaaaaaa agtaaatagt
     2161 ggattgaaaa gttagagtta cttaaattac ggtgtctaaa aaacacaaaa tcatccttaa
     2221 aataaaagat ctgacgctat ccaaatgagc ataggtagcg tctgccattt attcttagtc
     2281 aaattctttg acacctttct ctggttggat catctcaacg tttcttcaaa agaaagacca
     2341 taataattag gcaaatacat tttcttaaaa tgtgtcatat agatctagaa gcatcgcttt
     2401 gtagagattt gtgccgctag tcccctcaac aattactaaa tcaaatgtag gatttaccac
     2461 tttatctttg ataatcaacc tccctcttct gttgagacaa tgatctcgca atccgatgca
     2521 tactcctata taaactgcca gcaatcccca catattgagt agatcatgtc agaatgttct
     2581 atgtcgtcag caacgataca ctaaaagtca cgttcaccat gactgattgc tattcccagc
     2641 acaattattt ctatacacgt ttcatgattt gaataaacac tcacatcata catattacca
     2701 ttcatacacc atactgcagc ccctactata tggttgtaag ctcccaaatc tatatttgac
     2761 ttgatcactt tggtagtaga tctactaatt ctaaatccat tgctgataaa atttccattt
     2821 tttcctcctt ctttctgcca aaacagttaa aacaataata atctttatgt aaaaaagggc
     2881 tgtgacaaaa gccttgtcac agtcccatat tccgaataaa cggtggtcaa aagcttgcat
     2941 ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca tttcacaaaa atttaagaag
     3001 caattttcgt aaatttcttc ttatttcttg aagctaacca cttctgttac aaccactttt
     3061 agaacattta aagcttcgat gtgacaaacc atctatgctg ctcgatcata aaattgatag
     3121 caaaaattcc acaaagtaca tatgtccccc atgaaaacaa aacattcata tgcggtacaa
     3181 tgatctttgg aagaatcacg atcaaaaatg atgccagtgc ccaaatccat cgattgattt
     3241 gaatatgata tgtagctagc atattcaata taaggtgact agccgttaat aaaaagataa
     3301 aaacaaaaaa taaaatcagc caatacattg tttcccacct tctaaaaacc aaacttagac
     3361 agtctggact tcgttttctc cacgactttg ttcatctgac agcatctttt tatcataagc
     3421 ttttacaaat ggataataga taataaatgc tatcaatgtg ttgatcatga tcaagataac
     3481 aacacgccag tcatttcctg ctgaaagata accgccgatt ggagctggta atgtccatgt
     3541 tggcaaaaca gtaaatctat tgaccatttc taatttgact gcaaaataac tgataatagt
     3601 caaaactaaa ggaactaagt tgaacgggat gaacatgtaa ggattcaaca tcataggagc
     3661 tccaaaaatt atgggctcat tgatattgaa gataccagga ataatagaaa aacgaccaac
     3721 ctctttcaag aattttgatt tacagaagaa tataaacaaa acagatacca cgatcgttgc
     3781 tcctgcacca ccaattgtta ccgtccattg ataaaactgt tcaggagcga tatagggtaa
     3841 ttgggtggca gctgtaccat ctgccaatgc ctttgcattc tcatccaaca tgatcagcca
     3901 cattggacga acaacagccc ctacaatact catgccatgg ataccaaatg accacaacaa
     3961 atggatcaaa aacattggta gtaaaacacc aagcaaatta ttccctaaaa tattggttaa
     4021 aggctcgaat aacgataata aggcagcgtt tacatcaaag cccgcgacgc ttcgaatcag
     4081 ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc tcaaaactac gggcaacagc
     4141 aggtggcact tgttctggca ttttgatagt aatatttcga tttttaaaga aacgatatac
     4201 ttccactgca aaaatcatag ataagatccc cgcaaacatc ccagatgctc ccaaagaact
     4261 cataggtaag acccagccaa gaggtgcctc ttcaggagtt aaaacgttta caggaacttg
     4321 taacataaag aacgttccta aagataatat accacctgtc acactatcta atccatatga
     4381 tttagctaga tccgaaccca taacaaaagt tgcataaata gccatcaatc ccatcgtcat
     4441 cctaaagggt aaaacaatcg ttgccgaata aggggcaata aattcattcc atccaggaaa
     4501 cggtaaattg ataaagacca taaaaaatgt cccaatcaaa atcaatgaca atgttgaaac
     4561 aaccccatta cgaattgcta ataaatgacg ctgtgtacca atttttgcca ttggtgggac
     4621 aagtttctct tcgacccatt tcatcagctt ttccactaaa accactcctt ttcaataaaa
     4681 tatgaaccat tcattgcatg ac
//
LOCUS       DAZKOM010000149         4700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000149 DAZKOM010000000
VERSION     DAZKOM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.155"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8NF_002749"
     CDS             complement(543..2048)
                     /locus_tag="ACG8NF_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0473150.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8NF_002750"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8NF_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0473151.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8NF_002751"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8NF_002751"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0473152.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1330 a          964 c          776 g         1630 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttggctc
     4441 tataatttat gggactgatg aatcagaatt gattcttcag ccccattttt attttaggta
     4501 ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca actgagagga
     4561 cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag aaagatttga
     4621 tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc gtcgatgctg
     4681 ttttctcacc tgctccccat
//
LOCUS       DAZKOM010000150         4569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000150 DAZKOM010000000
VERSION     DAZKOM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.156"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            361..975
                     /locus_tag="ACG8NF_002752"
     CDS             361..975
                     /locus_tag="ACG8NF_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473153.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1140..1964)
                     /locus_tag="ACG8NF_002753"
     CDS             complement(1140..1964)
                     /locus_tag="ACG8NF_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473154.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2602..2859)
                     /locus_tag="ACG8NF_002754"
     CDS             complement(2602..2859)
                     /locus_tag="ACG8NF_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0473155.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2852..3121)
                     /locus_tag="ACG8NF_002755"
     CDS             complement(2852..3121)
                     /locus_tag="ACG8NF_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0473156.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(3252..3431)
                     /locus_tag="ACG8NF_002756"
     CDS             complement(3252..3431)
                     /locus_tag="ACG8NF_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473157.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(3447..3647)
                     /locus_tag="ACG8NF_002757"
     CDS             complement(3447..3647)
                     /locus_tag="ACG8NF_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473158.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(3812..4519)
                     /locus_tag="ACG8NF_002758"
     CDS             complement(3812..4519)
                     /locus_tag="ACG8NF_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473159.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
BASE COUNT         1444 a          840 c          629 g         1656 t
ORIGIN      
        1 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
       61 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
      121 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
      181 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
      241 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
      301 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
      361 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
      421 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
      481 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
      541 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
      601 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
      661 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
      721 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
      781 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
      841 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
      901 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
      961 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     1021 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     1081 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     1141 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     1201 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     1261 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     1321 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     1381 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     1441 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     1501 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     1561 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     1621 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     1681 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     1741 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     1801 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     1861 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     1921 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     1981 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
     2041 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     2101 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     2161 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     2221 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     2281 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     2341 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     2401 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     2461 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     2521 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     2581 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     2641 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     2701 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     2761 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     2821 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     2881 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     2941 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     3001 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     3061 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     3121 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     3181 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
     3241 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
     3301 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
     3361 tgctgcaatt ctttttgtct cttttcatcc aacgtcacgg gtaaccgttt atttcctatt
     3421 gcaattggca ttagtactct ctcctttcat cgaaataacc ctattaaaaa tatggctgca
     3481 atcaacttga atattagact cacaagggtt aacacaaagc gccaaaagat caaacaaaat
     3541 accagtattc ctaagaatac tacgaagttt aaatgtagag ctgcaccatg acgcataagt
     3601 atctcgccaa caaacagtac gaacaataga ggaatgattt tattcatatg gatctccttc
     3661 tttctcttag gttatcgggc tttgcccgct tatttcgcac cacctaagcg gcgggggcgg
     3721 ttggatcgat tggtgcgaaa taagaaccat tcccttaatt tcaaggtttt ttgttcttat
     3781 ttcacacatc ctcacttttc ggtataaatg gttattgacc ttttttcctt tcaaatcgtt
     3841 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtgt
     3901 aggtgtctaa aaaatcagtt aaagtcgtgt accagtcgtc gcctgggtta tctacttctt
     3961 ctacatattc cctcaaaaat gcttcgggta cagtgagttc ataatccatt cccattaaca
     4021 cgatagattc cgtcccacac gtcaaaaatc tagtaaatac aaggtaataa tcgtctaccg
     4081 tcgcatcttt gctgtttagc tcttccattt cttcttccat taattccatg ctctcaaaaa
     4141 ttgaataata ttctgattct gagtaatagt gtttcctgca aatcgtgtag ctgacaaagt
     4201 tttcgaccag ctcactcctt tctaggtaaa cattcatgat atatttttct gtttctagtc
     4261 gtagaagctc cgctcctttt tttaatgtgt caggacgacg tttatacacg tctaaacgat
     4321 tatggctgac tgtaaccttc gtttgactcg tttcactttc catatacaac tgttcttcca
     4381 ctctttgaat aaagtacttc atttcttcca tacaactaaa gtctttttga atccgtctga
     4441 ttacttcatt caagtgttct ttgtttgtta aaaattgcga aacatcttct gttgttagct
     4501 gataaaccac cttattcatt atctttctcc tccgattcaa tcgtataaaa tgcaccattt
     4561 ttttcgata
//
LOCUS       DAZKOM010000151         4475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000151 DAZKOM010000000
VERSION     DAZKOM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.157"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1439
                     /locus_tag="ACG8NF_002759"
     CDS             105..1439
                     /locus_tag="ACG8NF_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0473160.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1480..2391
                     /locus_tag="ACG8NF_002760"
     CDS             1480..2391
                     /locus_tag="ACG8NF_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0473161.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2453..3241
                     /locus_tag="ACG8NF_002761"
     CDS             2453..3241
                     /locus_tag="ACG8NF_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0473162.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            3238..3854
                     /locus_tag="ACG8NF_002762"
                     /pseudo
     CDS             3238..3854
                     /locus_tag="ACG8NF_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            3856..4374
                     /locus_tag="ACG8NF_002763"
     CDS             3856..4374
                     /locus_tag="ACG8NF_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0473163.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1343 a          763 c          902 g         1467 t
ORIGIN      
        1 aataattttt ttagaatcat accttataaa ttgttatttt cagaacaaaa tgatatcgtt
       61 atattaaaac cggcatcggt tacattaaaa tttggaggta aaatatgaaa aaattattgt
      121 ttacagggtt gctggcaagt tcagcattgt ttttattagg aggatgcgga tcaagtaatg
      181 caagcgatgc cagttcaagt gcagcaaatt cttctgaaaa aacaagtgat ggaagaaaaa
      241 atattgattt ctggtccttt tggggatccg gtgcacgaaa agaagctatc gaagaaatca
      301 ttgaggattt caatcaatca caaaacgaga tccatgtaaa ttataagtat cagccatggg
      361 gtgacatctg gactaaatct ttatctgcaa taacagctgg aaatgcaccc gatgttattg
      421 tacaggacat aaattccgtt gcccaaagag cagaagcaca gcaagcaact aatctctcca
      481 aatatgtaga aaaagacact tctaaggatt tttatccgca gctttgggat acagtagaat
      541 atgaaggtga tccttatgcg atcccattca atacagatac acaagtcatt ttttataata
      601 aaaagatatt taaagaagct ggaatcagtg aacaagatct accgacaact tgggatgaat
      661 tagaagaaac agcacgtaaa ctagataaaa aagaaggaaa agaatttaca cgcatcggtt
      721 tttatccatt atggaattta ggggctgatg tatgggcgct aaacgcagat aacgggacta
      781 gttggtttga caaagatgaa aaagtcaaga ttgatactaa aaataaagtg gaagccttgg
      841 agtggatcca agactggcaa gattattatg ggaaaaatac gatcaataaa ttagaagccg
      901 aatttgggtc tggtgtatct gatccattta tttccggatt agtagccatg cgtgcccaaa
      961 atatcaatta ttactctagt ttgatggaaa atgcaccgga tgattttgat tttggcgtga
     1021 tgcagctccc agaaaaagaa gaaggttcag gccattggtc gtggggtggc ggattcgtct
     1081 tggaagtacc atacggagca aaagatcctg aggcctctta tgaattcatc aaatatcttt
     1141 cttctccaga agtacaagaa aaattcggcg aaaaaagttt tgatatcatg gcgagcaaga
     1201 cagccaatga gaatttagtg aataatgaca acctaacgga taaaggaaaa atgatctatc
     1261 aaatggcgga cgaaaacttt aaaaatacgg tgatcacacc agttccttta gatgctccgg
     1321 attacacgac attagtgaat gaacagattg accagattct tttaggaaca aaatcaccaa
     1381 aagaaggatt ggcagatgca caaaaagcag tagaaaactt agtcaagcaa aatcaataat
     1441 ccatttttga ctgtctaggt acagaggagg caagaaaatt tgaagcgttt atctatccga
     1501 aagaaaaaag aagcaagatg ggcctattta tttatctcgc cttttattat cgggttcttg
     1561 gtatttatgc tagggcctat gcttttttca gttattggta gtttcaccga ttataatttg
     1621 acatccaaga tgaatttcat tggtctagca aatttcaaaa gaatgttcgt tcacgatgat
     1681 ttattttgga aatcgttata caacaccgtt tatttcgtca tcttcaatgt accgctgacg
     1741 acgattttca gtgtgttttt agccacagta ctcaatcaaa aaatcaaagg aatcagtgta
     1801 ttccgtacag ctttctattt accggcagtc ttatcaggag tagcagtcta catcttatgg
     1861 atgcagctgc tttctccatc tgcaggattg atcaacacat ttctgggctg gttccatatc
     1921 cctggaccag cttggctgtt tgatccacaa tggacgaaac ctgcgttgat tttgatgaaa
     1981 atatggagtt ctggaggagc aatgctactt tacttagcta cattacaaaa tgttcctaga
     2041 tctttatatg aatcagcaga aatcgatgga gcaggcgttt ggggaaagtt caaaagtatt
     2101 actttgccat taatcactcc tattattttt tacgatgtga tcacctctac gatcggttct
     2161 ttccaaattt ttcaagaagc ttatgtcatg acgaaaaatg gaacaggcgg acctgcaaat
     2221 tctttgcttt tttataatct gcatatgtgg aacaaggcgt tcgtagcttt tgatatgggt
     2281 tatgcgatgg ctatgtcaat gatcctgttt ctgatcgtgt tggttctgac gtttgtcaat
     2341 ttgaagcttg cacctaagtg ggtctattat tctggaggtg acggcaaatg atggattatt
     2401 tcaaaaagac cagtgtgaat cggcaaacaa aaaaatacta agttatgtcc tcatgacagt
     2461 aataggaatc attttgatca ttccattgct ttggatggtg tttacttcat tgaaaccaat
     2521 ggaagaaatc gttcgttatc ctccgacctt tttcccagaa aaaatcgtat gggaaaacta
     2581 tctggatacg attgccgcct ttcctttttg gcgctatgca agaaatacat tattcatcac
     2641 tgtgctagta gtgatcggta atgtcctttc aaattctttt attgcttatg gatttgcgaa
     2701 gcttgatttt cctgggaaaa agctgatgtt tgctttggtt ctttctacga tgatgatccc
     2761 ggggtttgta acgatgatcc ctcaatatgt attattctcc aagatcggct gggtaggtac
     2821 ttatttgccc ctgatcgttc catcgttctt cggcaatgcg ttcaatatat ttctgatgcg
     2881 tcaattttac ttatcgatca ataatgaatt gatcgaagct gctgaaatcg acggagcgaa
     2941 tcatttgtat atttggagtc atttgatgct tccattgacg aaacctgcct tgattaccat
     3001 tgcgatcaat tccttcaacg cagcttggaa tgatttctta ggtcctctgt tatatatcca
     3061 agatcaagaa aaatacacgt tacagattgg cttgcaagtt tttcagaacc aagcaacgac
     3121 acaatggaat tatctgatgg caggagcaac acttgtcttg atcccgacga ttttgctgtt
     3181 cttttttgca caacgttatt tcattgaagg gatggatttg acaggaggtt caaaaggatg
     3241 atgaatcgat cgattggtaa aaaagccaat gattatttct tagaaatcat tgaatggatt
     3301 ccacgtctta tccatttaca gctactatgg ttcctgtgcg tcttgcctgt tttcacatta
     3361 ggaactgcaa gtcgaacagt tctgtatatg atttatcatt acaaaaaaat gaagagaaaa
     3421 agctcggttc actattctgg aaaaagtttc gtgagaactt ctctctttat ggaaagcaag
     3481 atagtttagc aagtgtttat tttcttcttc tattgatcga ttatcagatt ttccgctatt
     3541 ggggtggggg aattggctat acattgatgt atactacgtt gtttttgctt ctattaagcg
     3601 cgcttctctt tagttacaaa atcttgcttc aactggaaca aaaagaagct tcttggatta
     3661 ctgcattttt cttgtttttt aatgatttca aaagcgcgct tttctatttg gcaggcacgt
     3721 tgatccttct tgtcgcattc tggtttgcag gaccaatcta ttttgcttta ttaggtttca
     3781 gtttcttgtt ttattttcaa gtcctgcttt ttatccatcg tacgcaagaa aaggttctta
     3841 aaaaggagga ataacatgtt gcgtttgatt gctaaaatcg atgatagtat tatcaacaag
     3901 ttcgccaagc aacggaaaac ggatagtcga aaaatcaatt ttttgaaatt tatcagtaat
     3961 atctctaatg gtggttttat ttggttgctc cttgctgctg ttttagtctt gatacctgat
     4021 actagagaat atggtctagc cattcttatc ggcagtttga tctatttttt tgttggtttg
     4081 ctttttatca aaaaaacgac aagacggaaa agaccttatg aaaaaaatcg tcggcttgtc
     4141 ctttacacaa gtaaaccgaa gacttcttct tttccttcag gacatacagc gctagccttt
     4201 ttatcagtag ctgcgttgtt cgaattaagt ccagttgcag gaatattagc ctttttattt
     4261 gcttgtttga tcgcttattc gaggattttt ttacttttac attatcctac tgacatttta
     4321 ggaggagcta ttttaggaag tgttattggc atcctatgcg ctgccgttat ctgaattaat
     4381 tgtacgaata aagcatgagt gcgtaaatgc gcatttgtgc tttatttttt tgtttcgagg
     4441 aaagcgtagt tgtttattca tttgtcttaa taact
//
LOCUS       DAZKOM010000152         4461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000152 DAZKOM010000000
VERSION     DAZKOM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.158"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACG8NF_002764"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACG8NF_002764"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFQ0473164.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACG8NF_002765"
     CDS             2298..3221
                     /locus_tag="ACG8NF_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0473165.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACG8NF_002766"
     CDS             3237..3776
                     /locus_tag="ACG8NF_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0473166.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAZKOM010000153         4297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000153 DAZKOM010000000
VERSION     DAZKOM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.159"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8NF_002767"
     CDS             <1..792
                     /locus_tag="ACG8NF_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0473167.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG8NF_002768"
     CDS             889..3138
                     /locus_tag="ACG8NF_002768"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0473168.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4297
                     /locus_tag="ACG8NF_002769"
     CDS             3141..>4297
                     /locus_tag="ACG8NF_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0473169.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1501 a          610 c          791 g         1395 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagt
//
LOCUS       DAZKOM010000154         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000154 DAZKOM010000000
VERSION     DAZKOM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.160"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8NF_002770"
     CDS             complement(37..1257)
                     /locus_tag="ACG8NF_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0473170.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8NF_002771"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8NF_002771"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0473171.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8NF_002772"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8NF_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0473172.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8NF_002773"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8NF_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0473173.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKOM010000155         4240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000155 DAZKOM010000000
VERSION     DAZKOM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.161"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..477
                     /locus_tag="ACG8NF_002774"
     CDS             40..477
                     /locus_tag="ACG8NF_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473174.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            572..1456
                     /locus_tag="ACG8NF_002775"
     CDS             572..1456
                     /locus_tag="ACG8NF_002775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0473175.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1678..>4240
                     /locus_tag="ACG8NF_002776"
     CDS             1678..>4240
                     /locus_tag="ACG8NF_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0473176.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1387 a          712 c          959 g         1182 t
ORIGIN      
        1 aagaaaacga tattaaaaaa tgcacgtaga agtagataaa tggagtggtt ttgcaaattt
       61 cttttatttc tagaagttat tcacgtatac ggatttgttt ttctcacaaa taaaaaggca
      121 ggaagtacaa tggatcattt gtatataaaa tatattcatc aattttccgt tttggtatta
      181 ggcacactaa ttatagcggg gattgttgca tttattattc aaggtcgtga catattaaca
      241 gtacatcaag agtttttcag ttatgcattt ggtattcctc tgatcgctat aaggtctgat
      301 aacaaaaagt ggaagaaacg ctattacatt ctcttgctat ttttgtgttt ttatctgcca
      361 cctaagattt ttactttatt aggtatacaa tgcctatcat tttcttttat ttcctatatc
      421 attgttttaa ttttcctcag tttcatagta tgggtatttt atcttgtcaa aaaataaatg
      481 aatgtttcat gaacagtact tcatcacagc ctaatttttt tctttatgct atcgttatat
      541 taaagaaatc aatcagaagg gaaagaaagt gatgaaaaaa gttttattag tggtaaaccc
      601 cagttcaggc ggtgaacaag caaaggaatt tgagcaatta gcgatagcaa aattggaatc
      661 ggtgtttgat gaagtggtcg tcttgcatac aaaaaaagca ggggatgcaa aaaactttac
      721 tcgcgaagcg gctacggaag ggtatcatag tgtgtttgtc atgggcggag atggaacagt
      781 caacgaagga atcagtggaa tcgctgaaca agaacatcgg ccaaatttcg gatttttccc
      841 attgggtacg gtgaatgact tggctcgagc attagggata ccgctagaac ctgaggaagc
      901 gatcaatcat ttttcgattg aatcagtcaa gccattggat attggaaaaa taaatgacga
      961 ctattttatg aatgtcgtcg caattggctc tatccctgaa gcaatcaatg atgtagatgc
     1021 ggagaagaag acgaaatttg gaaaactagc ttatttcatg tcaggaatca aacagttagc
     1081 ttccacacaa tcctactcgt ttcacgtaga agttgatgga aagaaggaag aaattgagag
     1141 cagtatcttg ctgataggac taactaactc agtaggcggt tttgaaacat tacttccaaa
     1201 tgcaaaagtg gatgatggta agctccattt ggtttattta aaagattctt ctttgatcga
     1261 tacgcttaaa acattgccag acctattaaa aggagtagat gaatctacga aaaatctagc
     1321 ctatcttact tgtgagcaat tagttgtgtc attagctgat gaggaagaat tgacgacgaa
     1381 tgttgatgga gatgaagggg ataaattacc tgtgacaatc aaagtgttac cctcacattt
     1441 acaggtttat tgttaaaatg gaggttgtga ccgaagagtt gtcaggagga catcggttgt
     1501 tcggaataag ggactgtgac aagacttttg tcacagcctt tttttgtaca aaaaagaaat
     1561 tttcacctaa cagaaaaatt tgccataaaa atacatattt taaatatgaa agtacaatta
     1621 caaataaatt aaaacgttaa aataaaattg tcaaaacgtc agagaggatg gtggagaatg
     1681 aatcaatatg taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg
     1741 acatccgttt ttgccgatgt agagaaaaca agcgaatcaa cgcaatcaga gaatcaaagt
     1801 tttgacaaga ctactttttc agaagaatca attacttctg aaactacaga atcccaaagt
     1861 gaggcgccta cttctaatac aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     1921 gctgaacaaa cgtctgatag cagtgaagag gtacaggtta gagttgtttc agcggatggg
     1981 tacagcgaag ctgccacaac agaagaactg acgcagcttc tcgcagacga atcagtagct
     2041 aagattcgtt taactcagcc tttgacttta gacagggaat tggagatcaa gcgagatatc
     2101 gttatcgatt ttggcggact tgctcataat ttcggcacac atcacatcta tatcaatgag
     2161 acacctcagt cgatcgagtt tcagaatttt aaaggaacgg cagctcatcc aggtgggttg
     2221 ataccaaata tcgatggaaa tgccattatc attgcttata tgtccgattt ttggggaaac
     2281 agatatcatt ttacgggaga aataaaattt accgggacac ttgacttata tgatggttct
     2341 aaattaggcc tgatttacgc ccctcgtgca actgtcacat tagatggtgt ttccggtgta
     2401 ttggatgtcc aaccagtgaa agaagggatg aatgccgctc cgggtaaagc ttatttttgt
     2461 agaagctatc agctaaatgt aatcaatggt agtcagcttt atggaccata tctaggaaaa
     2521 ttctatggtt ttctagattc aggtgatgag acgggcagtg gaaaaacagc accaggactc
     2581 catatcttga gtggttcaaa ggtctctctt gactatgatc gggcagatgg cgtcagcgat
     2641 gaaggagagg ctgtcgatac gctacccggg aatgtcacat ttaaagtcag tggctcagga
     2701 agtgaatttt cggtaaatac aaagatcaat attaccaacg acaacaatcg cggaattatc
     2761 caaatgagag gctcaggatc aagagtcgct gtgtcgaatg atgggaaaat cacaatcaat
     2821 acagcgacta ctggcggatt tcgcctgcaa ggagattcta gtcaaatcca tgtatcaagt
     2881 ggtggacaaa tctcggtcaa aatggctgga gatggcgacc aacctggaaa caatgggatg
     2941 cgttttgtag ggaaaggact ttctttgatc gtagaaggag ccggatcagt catcgatatt
     3001 gataagcaaa gtggtcgttc tagcgcagtt cgttttgaga acggtacgca aaaactaaca
     3061 gtaactaatg gaggctcatt gaaggtaaga aatgcagggg atggcaaatc ttatgtgaac
     3121 agactcaatc aaggaaacca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3181 ccagggtcag ctgattttac ggttactgga gaaaaatcaa atatcgatat acgcgcagat
     3241 tccggagcta ccgtagatac aactggagat attgatttga actttatggc tggcgaaaaa
     3301 acttatttag tcatggtcgg gaaaactggg gaagataaga ctggcatcat cagtggggat
     3361 gtgttgaatg tagacttaga atctcctacc tatttcgatt tccaaaacaa acggatcgga
     3421 agcgagacta atggcatagg tggctgggta tttcaaggaa atggcgattc ttctttgaag
     3481 atcgctcatt cagaaattgc tttatggcga aaggcaggag taacgttcga taactttgaa
     3541 aaagatccgg attatttttc tgatatgaca gacttatcga tcaatggaac cgacttaggt
     3601 aattttgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3661 atgaaaaatt ataatcgaat cagtgccaat aaccagcttc cgatgattga atccttaaga
     3721 gtaccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3781 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttatgca
     3841 gatacgacga aatcacctgt agaaattact gctctcacac aaggacaaac agatgaaaat
     3901 gaagggacag gatataatcc gtggggagaa ggcgaacaag gcggtttgtt tgaggttcaa
     3961 gtacccaacg aagaaagact aacaacgggg gatcaagtgc aaatagttgc tgctaaaaaa
     4021 gtaagaggcg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4081 cctcctgagc ctgctaaatt aagtaccaca ttgatcaatg cagcttctcg ctcaatcaca
     4141 ggtacaggcg aagcaggagc tttggtttca ttgaaaaaaa atcaagctgt tttgtccgaa
     4201 acaactattg atgatacagg taaatttgtt ctttctatcc
//
LOCUS       DAZKOM010000156         4228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000156 DAZKOM010000000
VERSION     DAZKOM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.162"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG8NF_002777"
     CDS             75..503
                     /locus_tag="ACG8NF_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0473177.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG8NF_002778"
     CDS             490..732
                     /locus_tag="ACG8NF_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473178.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG8NF_002779"
     CDS             complement(1032..1247)
                     /locus_tag="ACG8NF_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473179.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG8NF_002780"
     CDS             1354..1668
                     /locus_tag="ACG8NF_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473180.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG8NF_002781"
     CDS             1772..2950
                     /locus_tag="ACG8NF_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0473181.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACG8NF_002782"
     CDS             complement(3131..3316)
                     /locus_tag="ACG8NF_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473182.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACG8NF_002783"
     CDS             3353..3646
                     /locus_tag="ACG8NF_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0473183.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3880)
                     /locus_tag="ACG8NF_002784"
                     /pseudo
     CDS             complement(3734..3880)
                     /locus_tag="ACG8NF_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1448 a          633 c          821 g         1326 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaac
//
LOCUS       DAZKOM010000157         4069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000157 DAZKOM010000000
VERSION     DAZKOM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.163"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..2814
                     /locus_tag="ACG8NF_002785"
     CDS             145..2814
                     /locus_tag="ACG8NF_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0473184.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(3269..4015)
                     /gene="truA"
                     /locus_tag="ACG8NF_002786"
     CDS             complement(3269..4015)
                     /gene="truA"
                     /locus_tag="ACG8NF_002786"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0473185.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         1157 a          801 c          768 g         1343 t
ORIGIN      
        1 gtttattttt gttatacgaa caaattatga agttatttat tctaaatttt atttttttgt
       61 gaaaactaga ctctttttaa gcagagaaaa acaaggatat gataaactga atacaacttc
      121 atagaaattt ggtggaaaat aaatatgaaa aaaaatttaa agtcattctt tagagaaaac
      181 ggacgctacg cagcagctag tttcctgatt ccgtttttga ttttagcact tgtttatcta
      241 agtatcggaa tctatcctgg aagcagcaga agtattttgg caagtgatgc cttttcacag
      301 ttttctaatt ttcatgccag cttccgaaat gttcttcttg gaaagcaaag tattttttat
      361 acatggaatg cttctctagg attgaattat ttatctttaa tttcttacta tctaggcggg
      421 atcttcacgc cactggtgtt cttttttcca aaccagatga tgccagatgc actgtatgtt
      481 ctgactttgc ttaaggtcgg ctgtgccgga ttggcttttt ggtttcttgc acgtacgttc
      541 aagattcctc gctgggggca tgttgcactt agcgtcagtt acgcattgat ctcatttatc
      601 actgcacatt cggagatcat catgtggctg gatgcatttt tttatcttcc attagtgatt
      661 ttggggattc atcgtgttat ggatcttaaa cggccgaaac ttttatttat cagttattta
      721 ctcttgtttt tatccagttt ttatatgggt tttatgattg gcgtattttc tttcttatat
      781 tttatcgccc gtctattgac aaactggtca gaatataaaa aaacgattgt tccttatggt
      841 atcacttctt tactggcagg cggcgcttca atgatcattg tattgccagc agtgctggat
      901 cttcggtcaa atggcgaggc actgagtcag atcactacgt tgaaaacaga agcgacttct
      961 tatctggatc taatcatgaa aaatatgatt ggtgtttatg acacgacaaa atacggatcg
     1021 attccgttcg tttacgcagg tctgctacca ttagtcctct gtctgtttta ttttgccagt
     1081 cggaaagtac cgttgaaaaa taaatttttg tacggcagtc ttttcgccat acttatcgca
     1141 agtttttatc ttgttccatt gaatttgttc tggcatggta tgcatgcacc gaatatgttt
     1201 ttattccgtt atgcctatct gttctctttc ttagtgctcc tgcttgcagg aaaaggatgg
     1261 gaaaagatag aacaagacga tcgtgggctg ttgttaggaa tcattttggt cgtagctgct
     1321 ctctttgcgg tggcatgggg catcaaacct gaaggaagct acacctatgt gacgatgact
     1381 tcctttgtat tgacagtggt tttcttaggg atttatgctt ttgctgtggg attttatcag
     1441 cataaaaaac tttcattcaa tcatctgagt atattgttat tattgatcat gtctgctgaa
     1501 gcactggtca atacgaacag tatgatccat ggcattcttg atgattggaa ttatgcttcc
     1561 cgcagtttat atactgatcc ttatcctgac ttgaaagctc ttgtaaatga aacaaaaaaa
     1621 gattccgact cgttttaccg cttggaaaat ctggatccag tatctgcaaa cgattcgatc
     1681 aactatggtt acagtggtat cagtttgttt tcttctataa gaaatcgtaa ttcttcctct
     1741 tacctggata aattaggttt ccgttcaaga gggacaaatt tgaatatccg ttataacaat
     1801 aatactttgc tgatggatgg gtttacagga atcaaatata acatttctaa aaacgaaagc
     1861 acgttcagta aatatggctt ccgccaagct ggtcaaagcg gcgactatac actgtatcag
     1921 aatgtcaatg cattgccact agcatttact gcaccgttgt ccgttaatga tgttgagcag
     1981 ccagtgacag ataatttgac cagtcagaca aacttgatca atacattgtc tggtctaaat
     2041 gaacagtatt ttacattcta tacacctact ttgaagaatc agttgaatac gacgatcaca
     2101 aagacaacga ctggcgtcac ttactcagaa acagcagata accagccaaa agagctcact
     2161 tgggaagtcc atgtgcctgc aaatacacaa gcttacttaa gtttgttccc aaccaatttt
     2221 gcgcaattag aaagttctag tgcgacgatc actgtcaatg gtacatcgcg aaaaacgcaa
     2281 atcaacattt ctggtcaata ctatgatctt ggctattatc ctcaagagac aacagtgaca
     2341 tttactgcaa gtttttatgg gacgaaagaa ataagtttca tggagcctaa agtcgttggt
     2401 ctagatgttg tagcctatca agccgcaatg aatcagatca aagaaaaagg tgtcgaaatg
     2461 gaagccactg gcagaaaagc ttctgggact gtaaataccg ctgaagattc catgttggtg
     2521 acaactattc cttatgacaa aggatggaaa gctaaaattg atgggaaacc agtcaaggtc
     2581 agcaatttca aagacgcatt cttgatgata aaagttccag caggtaaaca tactgtcact
     2641 ttcagttact tgcctgaagg atttacgata ggtgcaatcc tttttgtcct atgtattgca
     2701 ttgtttattc tttatgtctg gtaccttgac cgcaatcgcc ccttttatgg cacgattgat
     2761 ccagcaatag aagaacaacc acgaaacaca cacagaagaa gacgtagaaa ataaagaaac
     2821 gatggaacct gttcagactg tccgcaccaa gggcaatcaa aacaacgttc catcgttttt
     2881 tggttacaga aaaaataggt aacaaaaaga tgattcactt atcaacgcct cgtccacaag
     2941 tcacgatatt catcaaagat gagaagctgt tttttgaaat tgctctttgt gactcagagt
     3001 taaatgacag gctcacgacc tctttcttaa acggtgttca atcagctgac cttcggtatt
     3061 agctcaacaa acggcaaaat atgaggagct attttcccgt ttgtttctct aatactcagg
     3121 tcaaaacgct gatttcacga cctcttcctt aaacggtgtt ccagaagtag cttcttcgaa
     3181 aataagccga aattttgcaa aattttgaga agtaattttc gtaaatacct tcttatttct
     3241 tgaagctgac cacttctatc ataacctctt attcatattt cacttcatac aaataaagtc
     3301 cttcagggtg tgctgtaggt cctgctgcat tacgatcttt tttcgcaata atatctggga
     3361 tactatcttc aggcaatcgg ccattgccaa ttttcaaaag cgttcctact aagatccgta
     3421 tcatcttata caagaagcca tttcccctaa atgtaaacaa caattcgtct tttgtttcat
     3481 ttacctccat cttagcctcg tagatcgtac gtattttatc ctctatcgag cttcctgatg
     3541 cacaaaaaga ggtgaaatcg tgagtcccta ataaatcagg aagcgcccgc tgaatttttt
     3601 ccaaatcaag tgggtaagga aaataacttg cataatggcg tcgaaaagga ctgcgtggtt
     3661 tccctatatc cactcgaaat tgatacgtct tttctttgac caaatatctt gcgtggaagt
     3721 cttctgaaac aatttctact tgtcttacag caatatcctc tggtgactgg gtatctagcg
     3781 cgaaacgcat tcgttctaac ggacgctcct ctggataatc aaaatggatt acttgcccga
     3841 tcgcatgtac tccggcatct gtccttcccg aaccaaagac cgtgatttct tttccatttg
     3901 ccattttttg taatgttttt tctacttctt cttgaacagt acgcccattt ggctgttttt
     3961 ggaatccatt aaaatttgtt ccgtcgtacg caatgattgc tttgtaacgt accatgtttt
     4021 tctctccttt gttcctaaaa ggttatctgg aataatgatt tttctataa
//
LOCUS       DAZKOM010000158         3989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000158 DAZKOM010000000
VERSION     DAZKOM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.164"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACG8NF_002787"
     CDS             complement(<1..302)
                     /locus_tag="ACG8NF_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0473186.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            395..744
                     /locus_tag="ACG8NF_002788"
                     /pseudo
     CDS             395..744
                     /locus_tag="ACG8NF_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1016..1306)
                     /locus_tag="ACG8NF_002789"
     CDS             complement(1016..1306)
                     /locus_tag="ACG8NF_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473187.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="ACG8NF_002790"
     CDS             1708..1944
                     /locus_tag="ACG8NF_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473188.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="ACG8NF_002791"
     CDS             1951..3903
                     /locus_tag="ACG8NF_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0473189.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1388 a          680 c          695 g         1226 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagtt
//
LOCUS       DAZKOM010000159         3935 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000159 DAZKOM010000000
VERSION     DAZKOM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.166"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACG8NF_002792"
     CDS             complement(242..391)
                     /locus_tag="ACG8NF_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473190.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ACG8NF_002793"
     CDS             853..1065
                     /locus_tag="ACG8NF_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473191.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ACG8NF_002794"
     CDS             complement(1343..3142)
                     /locus_tag="ACG8NF_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0473192.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ACG8NF_002795"
     CDS             complement(3266..3862)
                     /locus_tag="ACG8NF_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0473193.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1404 a          698 c          680 g         1153 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttg
//
LOCUS       DAZKOM010000160         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000160 DAZKOM010000000
VERSION     DAZKOM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.167"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG8NF_002796"
     CDS             complement(245..466)
                     /locus_tag="ACG8NF_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473194.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG8NF_002797"
     CDS             complement(564..809)
                     /locus_tag="ACG8NF_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473195.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG8NF_002798"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG8NF_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG8NF_002799"
     CDS             complement(2033..3034)
                     /locus_tag="ACG8NF_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0473196.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG8NF_002800"
     CDS             complement(3193..3459)
                     /locus_tag="ACG8NF_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0473197.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG8NF_002801"
     CDS             complement(3449..3805)
                     /locus_tag="ACG8NF_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0473198.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKOM010000161         3859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000161 DAZKOM010000000
VERSION     DAZKOM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.168"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG8NF_002802"
     CDS             <1..350
                     /locus_tag="ACG8NF_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473199.1"
                     /translation="FIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLK
                     PPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFP
                     NKNNNFYFYRNKL"
     gene            complement(476..1153)
                     /gene="nth"
                     /locus_tag="ACG8NF_002803"
     CDS             complement(476..1153)
                     /gene="nth"
                     /locus_tag="ACG8NF_002803"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0473200.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1160..1858)
                     /locus_tag="ACG8NF_002804"
     CDS             complement(1160..1858)
                     /locus_tag="ACG8NF_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0473201.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2126..3310
                     /locus_tag="ACG8NF_002805"
     CDS             2126..3310
                     /locus_tag="ACG8NF_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0473202.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3316..3759)
                     /locus_tag="ACG8NF_002806"
     CDS             complement(3316..3759)
                     /locus_tag="ACG8NF_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0473203.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1004 a          761 c          636 g         1458 t
ORIGIN      
        1 catttattat tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa
       61 gctctcatgc aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc
      121 tgatgagcct gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt
      181 tattatcact tatcattttt attacacctt tagatagata cgtcacttat ttatataatc
      241 ctttggaata ctggacttat tttataggta tattggaagg cattattttc tacatcgggt
      301 attttcccaa taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca
      361 tcctagctac tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt
      421 ttctccataa taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca
      481 ggcgtggttt cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac
      541 atattgatag caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac
      601 caaaaaaaat cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca
      661 gtgtttcttc tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga
      721 cacgttccac gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga
      781 catttgctgt ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca
      841 cttgcccatc gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat
      901 ttcgatacaa accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta
      961 acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat
     1021 ctgtcgcctg ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga
     1081 gttctccatg tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt
     1141 gtttgcttaa catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt
     1201 cttcttgttg agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt
     1261 ggtcttcttc cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc
     1321 ggtcaacgta attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca
     1381 aaatttccgg ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg
     1441 aagaaagcgg gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt
     1501 cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat
     1561 agatggggta aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct
     1621 tgataaatcc atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc
     1681 ccatatccat agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat
     1741 gcaactgtag aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa
     1801 gtaaaagatt agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga
     1861 tttctcctcc gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc
     1921 ttttctattt acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg
     1981 ttcatgtttt tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg
     2041 ttccttagaa tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa
     2101 cattattgca taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt
     2161 tggatacgtc tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc
     2221 gtttttaacg ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct
     2281 catgtctgtt tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga
     2341 tcattttggg cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt
     2401 gctttttggt ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt
     2461 gacggcagga gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga
     2521 aagaaccaaa gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc
     2581 tattataggg ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact
     2641 ctttactttc ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa
     2701 aaaacgttca gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt
     2761 attcaagatt tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc
     2821 tggctctctt caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg
     2881 gttggttggt ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat
     2941 tatgcctcga ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag
     3001 tgaaatcctc gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt
     3061 tttatttggg attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg
     3121 ccaactatcc aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag
     3181 tatacaatct ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc
     3241 accatttctt ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa
     3301 taaaaaatag tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg
     3361 aaatactgtg tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac
     3421 ccatttttga tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg
     3481 aaaatagatt tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat
     3541 cagttttttt gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt
     3601 tgtcacgtta ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt
     3661 aaatcctgca aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa
     3721 atccagatat tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt
     3781 cattatattg tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc
     3841 aatctgaaac caagaaaaa
//
LOCUS       DAZKOM010000162         3665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000162 DAZKOM010000000
VERSION     DAZKOM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.169"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..278
                     /locus_tag="ACG8NF_002807"
     CDS             <1..278
                     /locus_tag="ACG8NF_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0473204.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="ACG8NF_002808"
     CDS             421..642
                     /locus_tag="ACG8NF_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473205.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="ACG8NF_002809"
     CDS             658..1500
                     /locus_tag="ACG8NF_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473206.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="ACG8NF_002810"
     CDS             1563..3365
                     /locus_tag="ACG8NF_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0473207.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3424..>3665)
                     /locus_tag="ACG8NF_002811"
     CDS             complement(3424..>3665)
                     /locus_tag="ACG8NF_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0473208.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1308 a          519 c          746 g         1092 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaa
//
LOCUS       DAZKOM010000163         3652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000163 DAZKOM010000000
VERSION     DAZKOM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.170"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..782
                     /locus_tag="ACG8NF_002812"
     CDS             30..782
                     /locus_tag="ACG8NF_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0473209.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            779..1693
                     /gene="pfkB"
                     /locus_tag="ACG8NF_002813"
     CDS             779..1693
                     /gene="pfkB"
                     /locus_tag="ACG8NF_002813"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0473210.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1711..3642
                     /locus_tag="ACG8NF_002814"
     CDS             1711..3642
                     /locus_tag="ACG8NF_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0473211.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1174 a          659 c          835 g          984 t
ORIGIN      
        1 ctattatttg tattgaggtg atcgtgaaaa tgcttacaga ggaacgcaga caaaaaatcc
       61 tagacttgct tgaacagcag aaaatcgtga aatcacaaga tcttgtaaac cttttgaatg
      121 cttctgaatc aacgataaga cgtgatttac aagagctgga gaatagtggg tttctagaaa
      181 gagttcatgg cggagctaag aaagagcaat tattgaattt tgagcagaat atgagtgaaa
      241 aatcactcaa aaacgttcac gaaaaacagc aaatcgccaa atcggcagct gaaaccatta
      301 atgacgaaga tgtcatttat ttagacgctg gctctactac gttagaaatg attccttttc
      361 tgcaaggaaa acagatcaca gtagtgacaa attccgttca acatgcggca aatttagttg
      421 atttggagct tgctaccatt attttaggtg gaacgataaa gctggcaact aatgctgttt
      481 taggatctaa tgcgatagaa caactaaaaa attttcattt taataaagca tttatgggaa
      541 tgaacggtgc ccatcttgaa caaggattca cgactccgga tccagaagag gcagctgtta
      601 aacgtttagc catgaaaaat tccgaagaga catttgtatt gattgatcat accaaattca
      661 accagttgtc cttcacctct gttgcacctt tggaagcagc cacgatcata accgagcgtt
      721 gtccattaga attcgttaat gaattccgtg aaaagacaac aattaaggag gcaatacaat
      781 gatttacaca gtaacactta atccatcaat tgattatatc gtacgcgtag atgggttaaa
      841 actaggcgat ttgaaccgaa tgaaggaaga cttcaagtta cctggaggaa aagggatcaa
      901 tgtttcccgt attttgaaac ggatcgattc tcaatctaca gcactagggt ttttaggagg
      961 gttcactggc gaatttattt cgaagtggct tgaaaaagaa caaatccagt ctttgttcac
     1021 aaccgtaaaa gaagatacaa gaatcaatat caaactaaaa tcagacactg aaactgagat
     1081 caatggctta ggcccatcta tttcagatga agaaatcgat gagttgaagc aagcattagg
     1141 aacagtagga caaggagata tcgtcgtatt gtcaggaagc gcgccagcta gcctgcgaaa
     1201 aggattttat caagaactga ttgatatcat tcgagaaaaa ggtgcagatt tcgtgattga
     1261 tacaacaggt gatgacctaa aaaatgcact tgttaagaaa ccgttgttga tcaaaccaaa
     1321 caatcacgaa ttagctgagc tttacaacac aaccttccaa tctgtagaag aaatcattcc
     1381 gtatgggaaa aaattactag ctgaaggggc acagaatgtg ctgatctcaa tggctggaga
     1441 cggtgctttg ctcttcacgg aacaaggaac ttataaaacg aatgtattag ttcgcccatt
     1501 gaaaaattca gtaggtgcag gggattcaat gattgctggc tttattgggg cttacagccg
     1561 tacgaaagat ccaatcgaag cctttaaatg gggagttgct tgcggaagcg caacagcttt
     1621 ttctgatgac ttggcagctc gcgaattgat tgatgagttg ctgccagaag ttgaaatcac
     1681 aaaaattgat tagtttcaag gaggataatc atgaatatca aggatttaat ggtcaaagaa
     1741 gcgatgatca tggatttgca agcgactgat aaaaaaggcg caatcgatga aatggtccaa
     1801 aaaatgtttg atgccggccg gatttccgat atcgaaacat ataaagaagg aattctagcc
     1861 cgcgaagcac aaacttctac aggtcttggt gatggaatcg ccatgcctca cgcgaaaaac
     1921 agtgcagtta aagaagcaac agttttattc gcaaaaagcc aaaaaggagt agattatgaa
     1981 gccttagacg gacagccaac ttatctgttt ttcatgatcg cagctccaga aggcgcaaac
     2041 gacacacatt tgcaagcatt agcagcacta tctcgattgc tgattgatgc agatttcgta
     2101 ggaaaattaa aagaagcaaa tacaccagaa gaagtccaag acttgttcca aacagctgaa
     2161 gagcaaaaag aagcagaaga aaaagcagaa caagaaacac aagctgcaga agcttctgct
     2221 catccagaaa gaaaattcgt tgttgccgtt acagcttgtc caacagggat cgctcacact
     2281 tacatggcag aagatgcttt gaagaaaaaa gcgaaagaaa tgggtgtgga gatcaaagtc
     2341 gaaacgaacg gttcagaagg aatcaaaaac cgattgactg ctgaagacat cgcccgtgca
     2401 gaaggtgtaa tcgttgcagc agacaaaaaa gtagagatga atcgctttga cggaaaagaa
     2461 ctgatcaatc gtcctgtcag tgatggtatc cgtaaaccag aagagttgat caatctagcc
     2521 cttagtggta ctgcaccagt attccatggt gatggaaaag aaagcagttc agaagaagga
     2581 aatgcggatg gtactatcgg acaaagaatt tacaaagatt tgatgaacgg cgtatcacac
     2641 atgttacctt tcgttattgg tggaggaatc gctattgcgc tttctttcat gatcgaccaa
     2701 tttattggcg tgccacatga tcaattatca aatctaggga attacaatca agctgctagc
     2761 tggttcaacc aaatcggaca agctgcattt ggtttcatgc tgccggtatt agcaggattt
     2821 attgcttcaa gtatcggtga ccgccctggt ttgatcgttg gatttgctgc tggtgcatta
     2881 gctaatacag gcggtgcagg attcttaggt gcactgattg gtggattctt agctggttat
     2941 gtcatcgtat ttttacgtaa attattcaag aacttaccaa aatcattgga tggaattaaa
     3001 acgatcctct tttatccagt gtttggctta ctgattactg gtttcttgat gttgctggtc
     3061 aatgtcccta tgaaagcaat caatgatgca ttgaacggtt tcttgacaag tatgagtggt
     3121 tcgaatgctg ctttacttgg tgcattatta gctggaatga tggcggcaga tttaggcggt
     3181 ccaatcaaca aagccgctta tgtgtttggt acagcaactt tagctacgac tgttgcaact
     3241 ggcggaagtg tcgtgatggc ttctgttatg gctggaggaa tggtacctcc attggcaatt
     3301 ttcgttgcta cacgtttgtt taagaataaa ttcagcaaaa cagatcaaga tgctggtttg
     3361 acgaacatcg tgatgggact atcgtttgtc acggaaggat cgattccatt tgcagcagct
     3421 gacccgattc gtgccattcc aagttttatt attggttctg ctttaactgg cggattagtc
     3481 ggagctttcg gaatcaaatt actggctcct cacggcggga ttttcgttgt attcttactg
     3541 agtcacccgg tcatgtattt ggtgtttatt gctatcggag cagtcgtatc tggtgtgatt
     3601 tatggtttat tgagaaaagc tcctgcagta gcagaagcat agaaataaaa aa
//
LOCUS       DAZKOM010000164         3604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000164 DAZKOM010000000
VERSION     DAZKOM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.171"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..188
                     /locus_tag="ACG8NF_002815"
     CDS             <1..188
                     /locus_tag="ACG8NF_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473212.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            331..555
                     /locus_tag="ACG8NF_002816"
     CDS             331..555
                     /locus_tag="ACG8NF_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473213.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            570..1439
                     /locus_tag="ACG8NF_002817"
     CDS             570..1439
                     /locus_tag="ACG8NF_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0473214.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1420..2154
                     /locus_tag="ACG8NF_002818"
     CDS             1420..2154
                     /locus_tag="ACG8NF_002818"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0473215.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2187..3095
                     /locus_tag="ACG8NF_002819"
     CDS             2187..3095
                     /locus_tag="ACG8NF_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0473216.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3092..3572
                     /locus_tag="ACG8NF_002820"
                     /pseudo
     CDS             3092..3572
                     /locus_tag="ACG8NF_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1252 a          521 c          789 g         1042 t
ORIGIN      
        1 tagtagaaga tgcctccctc agtggcgaga acgagcgaat caatgagctg attgaattta
       61 tccgcaagaa caaattccgc accttagaat atgatgataa gcttgtaagg aagataatcc
      121 agagcgttac agtctatgaa ggccacttcg tcatagcctt caaatctggc atcgaaatgg
      181 aaatatgaaa accagaagta aatagcccat gactctacag tagagttgtg ggttttcttt
      241 tgcttgctga ttataataat attttaataa ctattgtgtt tatcaaccaa atggtttata
      301 atattcttag cgtaaacttg gaggtgctgt atgactacgg cagaaatgat taaagaactg
      361 tgtgagcaaa tgaatataag tgtttccgaa cttgctagac gtattggcca gactccacag
      421 aatttcaata aaaaattaca acgagaaaca gtaaccttgg atgagttgaa ggccattgct
      481 gatgtgctag gtgtcaagtt tgtgcaggca tttattttac cagatggaga agaaataaaa
      541 atatctaacg aataaaggag gatgtttata tggtaggtaa tgttattcaa atagtaacag
      601 agaaattatc atctttgcct tttatagaag gcatcgtatt agggggctca cgtgcaagag
      661 gcacccatac agaaaattct gatatagata tcggaatcta ttacaattca gactcatttg
      721 acctgacagc gattaatcaa attgctacag aattggatga taaaaataga aacaaccttg
      781 ttgtgcctcc cggagcgtgg ggtgattgga ttaatggcgg cggatggtta tttataaatg
      841 ggtatcatgt tgacttgatt ttacgtgata taaaacgggt agaacaaata atcaaagata
      901 cggagcaagg aattgttact gccaattatc agaccgggca tccccatggt tatataagtg
      961 caatgtatcg tggagaatta gcgattagta aaatactata tgctaagaac gaaagcttat
     1021 gcgaattaaa aaagcaggca gaaatttacc ctactgctct aaagaaaagc ttgatgaact
     1081 tttttatatt tgaagctgaa ttctctttaa tgtttgtaaa agcaaatgcg ggggtagagg
     1141 ataaatatta tattgcaggc catgtttttc gtattatttc atgcttaaat caagtactat
     1201 ttgcatgtaa taatgcttat tgcattaacg aaaagaaagc tataaaactg cttgaaactt
     1261 ttgaatataa acctgaaaaa tatgccgaaa gagttaatca tatttttgaa gtactcggtc
     1321 tttctctttt tgaatgctac gatatgaccg agaaacttta taaagaagtg aatgaaattg
     1381 tatcggagat aaataacttt ttaaacgagg agagttcaga tgaaagaaaa caaatatgat
     1441 gataatatat tttttcaaaa atacagtcaa atgagtcgct cgcagaaagg actggctggt
     1501 gcgggagaat gggagacttt gaaaaagatg ctacctgatt ttaagggtaa gcgtgtgctt
     1561 gatttaggat gcggctatgg atggcactgt atatatgcga tggaaaacgg tgcttcctct
     1621 gtagtaggtg ttgatatttc tcataaaatg ctcgaagtag caaaaggaaa aacccatttt
     1681 ccacagattg aatatgaatg ctgtgccata gaagatgtgg atttcccaga ggagagcttt
     1741 gatgtaatac taagttcgct tgcgtttcat tatgtagcag actatgagaa tttaataaaa
     1801 aagatatata ggatgctgaa ggctggtggc aatttagttt ttacagttga acatcctgtt
     1861 tttactgctc atggaacaca agactggtat tataacgaaa aaggagaaat actgcatttc
     1921 ccggtggaca attattatta tgagggcaaa cggacagcta tgtttttgga agaaaaggtt
     1981 acaaaatatc atagaacact gaccacatat ctaaatacac tgctttcaaa tagttttata
     2041 ataaatcaga ttgtggagcc acagccgcca gagaacatga tggatattcc ggggatggcg
     2101 gatgaaatgc gacgcccaat gatgctgatt gtatcggcaa aaaagaagat gtaataatat
     2161 agaaaaaata aacgaggagt atgtaaatga gatcagaaaa agaaatgatg gatttagtac
     2221 tttctttagc agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
     2281 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
     2341 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
     2401 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
     2461 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
     2521 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
     2581 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
     2641 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
     2701 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
     2761 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
     2821 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
     2881 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
     2941 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
     3001 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
     3061 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
     3121 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     3181 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     3241 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     3301 tgataagata atatatcttt tatatagaag atatcgccgt atgtaaggat ttcagggggc
     3361 aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc
     3421 atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt
     3481 gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     3541 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     3601 tctt
//
LOCUS       DAZKOM010000165         3540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000165 DAZKOM010000000
VERSION     DAZKOM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.172"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..970)
                     /locus_tag="ACG8NF_002821"
     CDS             complement(266..970)
                     /locus_tag="ACG8NF_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473217.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1157..1402)
                     /locus_tag="ACG8NF_002822"
     CDS             complement(1157..1402)
                     /locus_tag="ACG8NF_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473218.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1419..2270)
                     /locus_tag="ACG8NF_002823"
     CDS             complement(1419..2270)
                     /locus_tag="ACG8NF_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0473219.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2436..3440
                     /locus_tag="ACG8NF_002824"
     CDS             2436..3440
                     /locus_tag="ACG8NF_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0473220.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          603 c          687 g         1152 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata
     1141 atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat
     1201 aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca
     1261 gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg
     1321 catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat
     1381 tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg
     1441 tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt
     1501 aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga
     1561 agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca
     1621 tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga
     1681 ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat
     1741 tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt
     1801 gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca
     1861 tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa
     1921 gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc
     1981 aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt
     2041 gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt
     2101 tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct
     2161 ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc
     2221 gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct
     2281 ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg
     2341 tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt
     2401 tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa
     2461 ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc
     2521 tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc
     2581 aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc
     2641 aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag
     2701 aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga
     2761 tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag
     2821 acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac
     2881 aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa
     2941 aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg
     3001 ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg
     3061 gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg
     3121 atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg
     3181 aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag
     3241 aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat
     3301 tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt
     3361 tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg
     3421 aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag
     3481 ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg
//
LOCUS       DAZKOM010000166         3529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000166 DAZKOM010000000
VERSION     DAZKOM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.173"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACG8NF_002825"
     CDS             complement(<1..2303)
                     /locus_tag="ACG8NF_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0473221.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACG8NF_002826"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACG8NF_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          983 a          783 c          560 g         1203 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacat ttttataaaa cgacgaaaaa taaatcact
//
LOCUS       DAZKOM010000167         3502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000167 DAZKOM010000000
VERSION     DAZKOM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.174"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..437
                     /locus_tag="ACG8NF_002827"
     CDS             120..437
                     /locus_tag="ACG8NF_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0473222.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            434..595
                     /locus_tag="ACG8NF_002828"
     CDS             434..595
                     /locus_tag="ACG8NF_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0473223.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            598..897
                     /locus_tag="ACG8NF_002829"
     CDS             598..897
                     /locus_tag="ACG8NF_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473224.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            897..1211
                     /locus_tag="ACG8NF_002830"
     CDS             897..1211
                     /locus_tag="ACG8NF_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0473225.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1204..1734
                     /locus_tag="ACG8NF_002831"
     CDS             1204..1734
                     /locus_tag="ACG8NF_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0473226.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1731..2000
                     /locus_tag="ACG8NF_002832"
     CDS             1731..2000
                     /locus_tag="ACG8NF_002832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473227.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1930..2133
                     /locus_tag="ACG8NF_002833"
     CDS             1930..2133
                     /locus_tag="ACG8NF_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0473228.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2130..2417
                     /locus_tag="ACG8NF_002834"
     CDS             2130..2417
                     /locus_tag="ACG8NF_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473229.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(2450..2701)
                     /locus_tag="ACG8NF_002835"
     CDS             complement(2450..2701)
                     /locus_tag="ACG8NF_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473230.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            2904..3317
                     /locus_tag="ACG8NF_002836"
     CDS             2904..3317
                     /locus_tag="ACG8NF_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0473231.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         1304 a          517 c          784 g          897 t
ORIGIN      
        1 aaaaataaaa agtaaattat ataaaaaaga ataggttttt gggtctgacc ctccaaactc
       61 tccaccaaaa tcttcctaaa tgctttgata ccaacgatta aacagtctgg agggttacat
      121 tgaaatcaga acatcaaata caagacgaga tcagaattgc cttatcaaaa catggttgca
      181 cgatttttcg atccaatgcg ggacaagtga caacgatcga agggcggcaa ttttacggga
      241 tgccaaaagg gttcccagat ctatgtggac atcgtaattc tgatgggaaa gcaatttata
      301 tagaagtaaa aaatgaacgc ggtaagttgc gtgaagatca aaaaaggttt gctgaatttt
      361 taaacggtca acctgtacta tacggtgttg cacgatcagt ggaagaagca ttgaaaattg
      421 tggaggaatc atcatgacaa cagaagaagt gattcaaatg cgtattcgaa gccttcagcg
      481 tgagattgac gatctggaac ggacaaaggc agtgatggtc aatgaaacgg cgaggaaggc
      541 aatcgatttg cacatagaga atttaagaag ggaaatccat cgattggagg aatgagcgtg
      601 gataagaaag cagcaatgaa acgaatcatc gaactgacac attctgagaa ttggcaagaa
      661 gacaaagaaa tagttgcaga agtccaaaag ctcggtaaat caatgtggag tgaaaaaacc
      721 aaacggagaa caccgagaaa aattgcaatc tggcatggtg accgaattct agtaacaggt
      781 actgctgaac agttatctga aattactgga ttaagcaaaa acattatctg ggatagagct
      841 aaacgcgaga acgttgattc gaaaggtcgt caatttaaac attgggagaa gaaataatgg
      901 aggaactaat cacaaaagta gagcagtggg caaaagataa gggattggat caagctgatt
      961 ccagcaagca aatgttaaaa acgatcgaag agattgggga agttgccgct tctctagcta
     1021 gaaaagatga atatggttta agagacggaa ttggagacgt agtagtaacc ttgattattt
     1081 tagctatgca aaatgatatg gatttgtacg agtgtctgaa ccaagcatac aacgaaatca
     1141 aaggacgcac aggaaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggaggaca
     1201 gcgatgaata aaaaagtatt aattgataaa caagcattga ttgatgaatt aatgaaaata
     1261 cctggtgtgg gatctaatag tgacgcttta gaaacgatta aacgtttccc atcgtatgaa
     1321 ccgcagaagc cagttgtgcc taagtttgtg gcggaactac tcgactatta tcgtcagtca
     1381 acagatgttg atttattagc tttattgata actttccatg attggtatta tcgcaaaact
     1441 aaagacggtg agcatgaaga agcaattgat tggctagttg atcatcctga aatttatatg
     1501 cgtgcatggc tgtttggcta cgagctcgag aaagagccgt tgtattgcgt aataatcaat
     1561 ggtagatatc ttgtcaaagt attcagcaat acaaacgttg tcatattagt tccagcagat
     1621 gaattttcac agtacgttac tcaaaaattt caattaactg aaaaacaaat caaagcaatt
     1681 gatgaaagat actggccgtt tgctgtgcca gtggatgagg tggtagaagg atgagcgacc
     1741 catacattac taaattagaa aagttgtccg aagattttga gaagaataaa aataaactaa
     1801 ctaaagataa acttatagaa ctggttaggt tggttatcag aggagctatt acaagagaaa
     1861 aggcgcagca aaacatagaa atttttgctc gtagtgtccg agatggatta gagaagaaag
     1921 ttaaacaaaa tgaacaacag acaccgcaga ataacaaaac taagaaaacg ggaactgaat
     1981 gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg
     2041 gcaagtcagt gtgttgctga tgcgagcggc gctattcgta agtttggaat ttcgatatta
     2101 agtgatgatc gtaaatggga ggaaaaagaa tgaaactaaa agacggattt tactccagca
     2161 gtcatggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa acacgtgaga
     2221 aaccaaaatt caaagtcggt gacatggttc gctgtgaagc agaaggattc atctatccat
     2281 tttgtggata tgtagaacat ctctataatc actcagcaat cattcgtatt gaaaacacga
     2341 tggaatgtga caagtggtta gcgaaaagca aagagaattt agcagtagcg agattggtgg
     2401 atatggaggt tatataaaca gcgaaaaaat gtacttctaa gagaaaaatt tattttttat
     2461 aaaaatagga aagtatcatt atgaaaaaga ccacagagat aaataaacct accacataaa
     2521 aaatgaatat ttcttgcgat tcaatatagg tatatttaac aatttgtaca ataaaaaata
     2581 ctataggtaa aaaagataac atgaggagct tatttgttct agagtgtttg acaaagtaac
     2641 aaaatgcgac gataccaata caaacgggaa tccaaaataa cagcatgctc cacatagtca
     2701 ctcaaccatc tcctatacgc tttattatca atagataata tcatttttcg taataaagat
     2761 aaaaaaataa gtaaggcttc tcattaaaat ataaaaaaga cagccgacca ctgactgcct
     2821 atataagagt attgaaataa aaagctgctg atataataaa ttccacaagt ttattatatc
     2881 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     2941 gatgtaatgc gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct
     3001 ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcgccg aagcacggca
     3061 ataaggcaga agacgcaatc attcagatga tggatataga agcggagaga gacgcgattc
     3121 tagcagcctt gatggctctt agtctgatta gtcgtcagat actctactat agcttttgtg
     3181 tgccagatag cttctcaaac tacagaatta gccgtgaagt gggttattca gaaagaagta
     3241 tacaacggat gaagtcggaa gctctaatag agtttgcaga agcatataaa cacggaagaa
     3301 taattgctta taaataattt ggcggctttt tggcggaatg atggcggttt ttagctattt
     3361 accagtgata ttatggtagt gtcgaaagat tagtgatagg tctaagacaa aataataata
     3421 aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata
     3481 ttaagaataa ggatgtgaat tt
//
LOCUS       DAZKOM010000168         3351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000168 DAZKOM010000000
VERSION     DAZKOM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.175"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8NF_002837"
     CDS             278..787
                     /locus_tag="ACG8NF_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0473232.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8NF_002838"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8NF_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0473233.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8NF_002839"
     CDS             2322..3302
                     /locus_tag="ACG8NF_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0473234.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
BASE COUNT         1198 a          536 c          659 g          958 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga a
//
LOCUS       DAZKOM010000169         3225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000169 DAZKOM010000000
VERSION     DAZKOM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.177"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..391
                     /locus_tag="ACG8NF_002840"
     CDS             188..391
                     /locus_tag="ACG8NF_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473235.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="ACG8NF_002841"
     CDS             602..1186
                     /locus_tag="ACG8NF_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473236.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1557..2882
                     /locus_tag="ACG8NF_002842"
     CDS             1557..2882
                     /locus_tag="ACG8NF_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0473237.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2875..3225
                     /locus_tag="ACG8NF_002843"
     CDS             2875..3225
                     /locus_tag="ACG8NF_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473238.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1150 a          472 c          650 g          953 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca ctttcttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttatta tcaaatgttg taaggatttt attcatttgt taatattaaa
     1321 gaacggaact ccaaaaaagt ccgtactcta atttgcagtc tctcactctt ttagagtgag
     1381 agtttttatt agattttcaa attaattagt aaagtgatag aataaattga tcatacaaat
     1441 cataagaaga agcagggtga tagcatacct atgcttcttt ttttatgtat ttagctattt
     1501 cttctttaca acatcgaaca tatgtttgta taatgtggaa gaatggagtg ttatttatga
     1561 tggattattc aaaagagcca gtaaatgatt actttttaat cgatatgaaa agcttttacg
     1621 caagtgtcga atgtatagaa agaaatttag atccattaac aacagaactt gttgttatga
     1681 gtcgatctga taatactggt tcaggattga tattagcttc ttctcctgaa gcaaaaaagc
     1741 ggtatggtat tacaaatgtg agtagaccac gtgacttacc acaaccattt cctaaaacac
     1801 tacacgttgt tccaccacgt atgaacctat atatcaagcg aaatatgcag gtaaataata
     1861 ttttcagaag atatgtggct gatgaagatc tactgattta ctcgatcgat gaatcgattt
     1921 taaaagtgac aaaatcactg aatcttttta cgactgaaga aacacgaagt cagcgaagga
     1981 agaagctcgc tcaaatgatc caagaacgta ttaaagaaga gttaggtttg attgctacgg
     2041 taggcgtcgg cgataatccc cttttggcta aattagcctt agacaatgaa gcaaagcata
     2101 atgaaggatt tattgcggaa tggacgtacg aaaacgttcc tgagaaagta tggaatattc
     2161 ccgaaatgac ggacttttgg ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg
     2221 gaattttgag cattagagac cttgctaact ggaatcctta tacgattaag aatagattag
     2281 gtgtgattgg attacagctg tacttccatg ctaacggaat cgatcgaaca gatatagcaa
     2341 tcccgccaga accgacaaag gaaaaatctt atggtaattc acaagtgtta ccacgagatt
     2401 atactagaag aaatgaaatc gagctggtgg taaaagaaat ggcggaacaa gtagccattc
     2461 gtatcaggca gcataattgt aaaacaggat gtgttcattt aaacattggt acgtcgatct
     2521 tagaaacgag accaggattt tcacaccaga tgaaaatacc tatcacagat aatacaaagg
     2581 aattgcagaa ttattgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc
     2641 acgttggaat aacctatagc aagcttgtgt acaccgattc gcttcagctg gatttattta
     2701 gtgatccaca aaagcaaatt aacgaagaaa atttagataa aattattgac aagattcggc
     2761 aaaaatatgg ttttacgtcg attgtacatg cttcttcaat gttagaaagc gctagaagca
     2821 ttactagaag cacacttgtt ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt
     2881 gattacaatg atcgtggacg tttaaagtgg gcagggtttt atttatctga tcatactgaa
     2941 gaaattgatg ccgacaatga acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg
     3001 actgaagaga ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatctatt
     3061 caaaaagaag aacgaaacgc tgaaggatac tatccagaag atattgttgg caagatctta
     3121 ggtagtgatg agctggggat ctacgttgga aaagacaaaa ttcactacga cgaaatacgt
     3181 catatcgaat tttaccagga taataaatgg agtcgattag aatga
//
LOCUS       DAZKOM010000170         3202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000170 DAZKOM010000000
VERSION     DAZKOM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.178"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8NF_002844"
     CDS             <1..1412
                     /locus_tag="ACG8NF_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0473239.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACG8NF_002845"
     CDS             1546..2889
                     /locus_tag="ACG8NF_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0473240.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..>3202
                     /locus_tag="ACG8NF_002846"
     CDS             3001..>3202
                     /locus_tag="ACG8NF_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0473241.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESST"
BASE COUNT         1270 a          443 c          612 g          877 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac ga
//
LOCUS       DAZKOM010000171         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000171 DAZKOM010000000
VERSION     DAZKOM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.179"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8NF_002847"
     CDS             301..585
                     /locus_tag="ACG8NF_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473242.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8NF_002848"
     CDS             588..1004
                     /locus_tag="ACG8NF_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473243.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8NF_002849"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8NF_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0473244.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG8NF_002850"
     CDS             2522..2608
                     /locus_tag="ACG8NF_002850"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0473245.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG8NF_002851"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG8NF_002851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473246.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKOM010000172         3041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000172 DAZKOM010000000
VERSION     DAZKOM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.180"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..752
                     /locus_tag="ACG8NF_002852"
     CDS             138..752
                     /locus_tag="ACG8NF_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473247.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="ACG8NF_002853"
     CDS             complement(776..1126)
                     /locus_tag="ACG8NF_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0473248.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="ACG8NF_002854"
     CDS             complement(1120..1383)
                     /locus_tag="ACG8NF_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0473249.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(1724..1903)
                     /locus_tag="ACG8NF_002855"
     CDS             complement(1724..1903)
                     /locus_tag="ACG8NF_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473250.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(1919..2119)
                     /locus_tag="ACG8NF_002856"
     CDS             complement(1919..2119)
                     /locus_tag="ACG8NF_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473251.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            complement(2284..2991)
                     /locus_tag="ACG8NF_002857"
     CDS             complement(2284..2991)
                     /locus_tag="ACG8NF_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473252.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
BASE COUNT          925 a          589 c          469 g         1058 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacttg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaaat tgatctaaaa ataatctctt ctggatactt taaagatttc
     1621 atagttattg atacaaaatg gaataaaaag gacatagaaa atattctaaa tagtaggaac
     1681 aattaagtgc tatcactttt gtttcttcaa atgaaaagtg acgtcacttt tttagtgctg
     1741 gtatatcaaa tcctgctttt tcttgcgcga tcaatagatc taacgcaata cacataatct
     1801 tagattcact ttttccgtac ttctgcttta gttgctgcaa ttctttttgt ctcttttcat
     1861 ctaatgtcac gggtaaccgt ttatttccta ttgcaattgg cattagtact ctctcctttc
     1921 atcgaaataa ccctatgaaa aatatggctg caatcatctt gaatagtaga ctcacaaggg
     1981 ctaatacaaa tcgccaaaag atcaaacaaa ataccagtat tcctaagaat actacgaagt
     2041 ttaaatgtac agctgcagca tgacgcataa gtatctcgcc aacaaacagt acgaacaata
     2101 gaggaatgat tttattcata tggatctcct tctttctctt aggttatcgg gctttgcccg
     2161 cttatttcgc accacctaag cggcgggggc ggttggagcg attggtgcga aataagaacc
     2221 attcccttaa tttcaaggtt ttttgttctt atttcacaca tcctcacttt tcggtataaa
     2281 tggttattta ccttttttcc tttcaaatcg ttgatatggc attcgtttac ggtcaagttc
     2341 attttggatt tcttctgtat cgtcgtaagt gtaggtgtct aaaaaatcag ttaaagtcgt
     2401 gtaccagtcg tcgcctgggt tatctacttc ttctacatat tccctcaaaa atgcttcggg
     2461 tacagcgagt tcataatcca ttcccattaa cacgatagat tccgtcccac acgtcaaaaa
     2521 cctagtaaat acaaggtaat aatcgtctac cgtcgcattt ttgctgttta gctcttccat
     2581 ttcttcttcc attaattcca tgctctcgaa aattgaataa tattctgatt ctgagtaata
     2641 gtgtttccta caaatcgtgt agctgacaaa gttttcgacc agctcactcc tttctaggta
     2701 cacattcatg atgtattttt ctgtttctag tcgtagaagc tctgttcctt tttttaccgt
     2761 ttcaggacga cgtttataca cgtctaaatg attatggctg actgtgacct tcgtttgact
     2821 tgtttcgctt tccatataca actgttcttc cactctttga ataaagtact tcatttcttc
     2881 catacaacta aagtcttttt gaatccgtct gattacttca ttcaagtgtt ctttgcttgt
     2941 taaaaattgc gaaacatctt ctgttgttag ctgataaacc accttattca ttatctttct
     3001 cctccgattc aatcgtataa aatgcaccat ttttttcgat a
//
LOCUS       DAZKOM010000173         3036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000173 DAZKOM010000000
VERSION     DAZKOM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.181"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="ACG8NF_002858"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="ACG8NF_002858"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0473253.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="ACG8NF_002859"
     CDS             complement(1032..1952)
                     /locus_tag="ACG8NF_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0473254.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /locus_tag="ACG8NF_002860"
     CDS             2065..2817
                     /locus_tag="ACG8NF_002860"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0473255.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          855 a          612 c          526 g         1043 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagat
//
LOCUS       DAZKOM010000174         2891 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000174 DAZKOM010000000
VERSION     DAZKOM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.182"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            325..669
                     /locus_tag="ACG8NF_002861"
     CDS             325..669
                     /locus_tag="ACG8NF_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0473256.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            670..1089
                     /locus_tag="ACG8NF_002862"
                     /pseudo
     CDS             670..1089
                     /locus_tag="ACG8NF_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1181..1531)
                     /locus_tag="ACG8NF_002863"
     CDS             complement(1181..1531)
                     /locus_tag="ACG8NF_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473257.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1497..2828)
                     /locus_tag="ACG8NF_002864"
     CDS             complement(1497..2828)
                     /locus_tag="ACG8NF_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0473258.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1058 a          517 c          554 g          762 t
ORIGIN      
        1 attttgaaat aagactctat tattacaagt tcatccgttg taagatgggt ataggtcatt
       61 tgtactcact ccttataatt ctttggtcgg aactattttg agtgtagcac aaatgacttt
      121 tttagttgtc tagcttaatt ttacaatcgg cgaataaaaa aagcgttagc aactatgctt
      181 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      241 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      301 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
      361 ggagattttg caccaaaatt tgcagaattg aatgatgatg ttttatttgg acaagtatgg
      421 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
      481 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
      541 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtaggatg gccaaaagct
      601 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
      661 gaggcgtaaa tgacgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
      721 gaaaaaaacg aagcttacgc tcaagtattt accgggtaaa gctatctgaa tatgttagtg
      781 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
      841 cactggcatg tccataaaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
      901 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
      961 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1021 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1081 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1141 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1201 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1261 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1321 gataatagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     1381 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     1441 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     1501 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     1561 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     1621 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagtaca
     1681 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcgag acctgtccca
     1741 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     1801 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     1861 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     1921 aaaacataag aagacagcat cagaccaact aagcctatgt tgatcaaagt agcaccaata
     1981 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2041 cccaaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2101 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2161 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2221 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2281 agaagtagac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     2341 aaacgcatac caaaagtgat ataactaacg accggtgata atgcgctacc aaacagtccg
     2401 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     2461 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     2521 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     2581 agtaaggtca aaatccctac atttatgaat gcgcttccga aaccgcctaa agcaaaataa
     2641 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     2701 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     2761 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     2821 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     2881 gacaactatc a
//
LOCUS       DAZKOM010000175         2716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000175 DAZKOM010000000
VERSION     DAZKOM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.183"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..503
                     /locus_tag="ACG8NF_002865"
     CDS             57..503
                     /locus_tag="ACG8NF_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0473259.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            776..2128
                     /locus_tag="ACG8NF_002866"
     CDS             776..2128
                     /locus_tag="ACG8NF_002866"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0473260.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2299..2586
                     /locus_tag="ACG8NF_002867"
     CDS             2299..2586
                     /locus_tag="ACG8NF_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0473261.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          957 a          447 c          540 g          772 t
ORIGIN      
        1 attttaaaat gtaaaaatag tatgataaga cgagaataac aacgtggggt gtttacatgg
       61 aaaataagtt agtcgaagag gttatcgaaa agttaaaaaa agagaacatt cgaatcacac
      121 ctcaacggta cgcggtttta gaatatttga ttgaacatca ttcccatcca acggctgatg
      181 agatttataa agcacttgaa catcggtttc caaatatgag tgttgcgact gtttacaata
      241 accttcgctt atttacttcg attggatttg ttcaagaaat gaagtacggc gatgcttcta
      301 gccgatttga tttcagttca aagcgtcatt atcatgtaat ttgtgaaaac tgcggtaaga
      361 ttgttgattt tcattaccca gggcttgaag atatagaaat ggctgcaagc agactgactg
      421 attttgagat taatgaacat cggttggaat tgtatggact ctgtccggat tgtaaagagt
      481 cagcaaaaaa ggcgcaaatg taaaaacagt ttctacaagt gttataccta aaaaaagcta
      541 gctgtgtaaa aaaaacgcta gcttttttat ttttttgtct tttattcaca atcttagata
      601 taaaagtgaa tattattaaa ataagaatct tgtagatatt gggttcttat gcttgaaaag
      661 gaatgaaact acaaacaaac ctttgtgaat tcacaagcat taaactgttg tatccgtttt
      721 atttctgtta tagtagtttt gtcgaaggac aaaatgttaa tcggagggac cataaatgaa
      781 agtagtagta attggctgta ctcatgcagg tactgcagct gtaaaaagta ttttaaaaaa
      841 tcatccagaa gcagaagtga cagtatatga aagaaatgac aatatttctt tcttatcttg
      901 cggtatcgca ctttatgtcg gcggagtagt aaaggaccca gcaggcttgt tctactcaaa
      961 tcctgaagaa ttatcttcat taggagcaac agttaaaatg gaacacgatg taacagacgt
     1021 cgatacagaa aataaaacag taacggcaaa agatctgaaa acaggagaaa cagaaacggt
     1081 ttcttatgat aaattagtga tgacaactgg ttcatggcct attatcccac cgattagtgg
     1141 aatcgatgcg aaaaatgtac tcctatgcaa aaactacaat caagcaaatg aaattattgc
     1201 acaagcgaaa gaagcaaaac gtgtagtaat tgttggtggt ggatatatcg gtattgaatt
     1261 agtagaagct tttgtcgaat ctggaaaaca agtgacattg attgatggat tagatcgcat
     1321 cttgaataaa tatctagata aaccattcac agatatctta gaaaaagaat tgacagaccg
     1381 cggtgttact cttgcgctag gcgaaaatgt tactgaattt atcacagatg aagaaggaaa
     1441 agtgaaacaa gtagctacac caacagatac atttgataca gatatggtca ttttatgtgt
     1501 gggcttccgt ccaaatacaa aactgatcga aggaaaagta gatacactgc caaacggggc
     1561 aatcaaagtg aatgaataca tgcagacaag taatccagac atttttgcag ctggagactc
     1621 tgcagtagtc aactacaatc caagtggcac gcaaaactat attcctttgg caacaaatgc
     1681 cgttcgtcaa ggtttgcttg tgggaaataa cttgacagat cataaaatgg cgtatcgtgg
     1741 tactcaagga acttcaggac tttacctatt tggctggacg atcggttcga caggtgtgac
     1801 caaagaaagc gcaactttga acgatctaga agtccaagcg actgtatttg aagataatta
     1861 tcgtccagag ttcatgccaa ctactgaaaa agtaatgatg gagctagtct atgaaaaagg
     1921 aacgaatcgt atcgtaggtg ctcagttcat gtccaaatac gacattacac aatcagcaaa
     1981 taccatgtct ttagctgttc aaaataaaat gactgtagaa gatctcgcac tttcagattt
     2041 cttcttccaa ccacattttg accgcccatg gaattacttg aacctacttg cacaagcagc
     2101 gctaggcgaa atcgaaactg cacaataaag aaaaagggtt gtgacacaat tttgtcacga
     2161 ccctctgtct ttttttgtga aaagtacaat tagttgtctt tcttcgaatt ttttgtcaca
     2221 ataagcagca aagataaagc tgttacataa aagaacaagc agctttattt gattttgaaa
     2281 agaaaggtga gaaagaagat gaataaagaa tattttgtcc aacaagatgc atttttagaa
     2341 gattataaaa gtgcttcgaa gaaattaacg atcaatattg atgagttggc agatcaagta
     2401 ctctctcagt tgaatgaaac gcaagaggaa tattacacga tgccagcaaa tgacgccaaa
     2461 gataatgttt ctcatcgttt tcctttcagt agacgcatct actcagatga aaatgacggc
     2521 acattgaata cagagtacac atatgaaggt cagccatatg tgctagaaac tgatgatgat
     2581 ctttaaagac agtttttgaa aaggccgctc atccttaagt gatgacaaaa aatcatcaca
     2641 attaccgatg actgtctctt tactatcttt taatcagaaa aaggggttat gacaagcttt
     2701 gtcaacccct tttttt
//
LOCUS       DAZKOM010000176         2461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000176 DAZKOM010000000
VERSION     DAZKOM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.184"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8NF_002868"
     CDS             <1..189
                     /locus_tag="ACG8NF_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0473262.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1480)
                     /locus_tag="ACG8NF_002869"
     CDS             complement(1055..1480)
                     /locus_tag="ACG8NF_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0473263.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="ACG8NF_002870"
     CDS             complement(1582..2199)
                     /locus_tag="ACG8NF_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0473264.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          810 a          469 c          274 g          908 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 t
//
LOCUS       DAZKOM010000177         2458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000177 DAZKOM010000000
VERSION     DAZKOM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.185"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8NF_002871"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8NF_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8NF_002872"
     CDS             1320..1778
                     /locus_tag="ACG8NF_002872"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0473265.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG8NF_002873"
     CDS             1937..2407
                     /locus_tag="ACG8NF_002873"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0473266.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DAZKOM010000178         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000178 DAZKOM010000000
VERSION     DAZKOM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.186"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8NF_002874"
     CDS             207..2069
                     /locus_tag="ACG8NF_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0473267.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG8NF_002875"
     CDS             2069..>2400
                     /locus_tag="ACG8NF_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0473268.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZKOM010000179         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000179 DAZKOM010000000
VERSION     DAZKOM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.187"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8NF_002876"
     CDS             316..570
                     /locus_tag="ACG8NF_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0473269.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8NF_002877"
     CDS             571..888
                     /locus_tag="ACG8NF_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0473270.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8NF_002878"
     CDS             1447..2382
                     /locus_tag="ACG8NF_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473271.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKOM010000180         2311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000180 DAZKOM010000000
VERSION     DAZKOM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.188"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..721
                     /locus_tag="ACG8NF_002879"
     CDS             527..721
                     /locus_tag="ACG8NF_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473272.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            912..1161
                     /locus_tag="ACG8NF_002880"
                     /pseudo
     CDS             912..1161
                     /locus_tag="ACG8NF_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1405..2235)
                     /locus_tag="ACG8NF_002881"
     CDS             complement(1405..2235)
                     /locus_tag="ACG8NF_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0473273.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          692 a          420 c          461 g          738 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcgctc g
//
LOCUS       DAZKOM010000181         2282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000181 DAZKOM010000000
VERSION     DAZKOM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.189"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1561
                     /locus_tag="ACG8NF_002882"
     CDS             <1..1561
                     /locus_tag="ACG8NF_002882"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0473274.1"
                     /translation="KIESLEPYDMIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMI
                     YQMGMEYAGLNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKY
                     FSIIGWIVFTLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLI
                     YAHQRNDVINVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQK
                     MYPDYTPVGELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQN
                     YYFIISAIMSIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFI
                     TLFQPFMIIWVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLIT
                     AMINIVLNIILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLI
                     SLCVIAFVASYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYI
                     LKVLTSVLVKR"
     gene            1739..1960
                     /locus_tag="ACG8NF_002883"
     CDS             1739..1960
                     /locus_tag="ACG8NF_002883"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0473275.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          772 a          239 c          435 g          836 t
ORIGIN      
        1 aaaaatagaa agtttggagc catatgatat gataattaat cgtaaacaaa atgctaaaag
       61 gaatataatt actggaacaa tttttaagtt atatcagatt atcattccct tttttatgcg
      121 tacacttatg atttatcaga tgggaatgga gtatgctgga ttgaacaacc tgttcacatc
      181 tatatttcaa atgttaaatt tagcggaatt gggaataggt gctgcactaa ctgtaagtat
      241 gtataaacct atagcagaag acaatagaaa agagatttgt gctttactga acgcatataa
      301 aaagtacttt tcaattattg gatggatagt gtttacatta ggattgttat gcattccttt
      361 tttgagattt ttagttaaag gttcgattcc tggaaattta aatatgacag ttttgtattt
      421 aatgtatttg ataaatactg ttttgtcgta ttggttattt tcatatagaa agagtctaat
      481 atatgcacat caaagaaatg atgtaattaa cgtgtctatg ctaattactt ttacattcca
      541 atttgtaatg caggcatatg ttctgatttt cttaaaaaat tactatttgt atttattggc
      601 ttctattttg ggacaaatag ttcttaactt aatgtgtgct gttgtttctc aaaagatgta
      661 tcctgattat actcctgttg gtgagttaga gaaagaaact attgacgata ttataaaaaa
      721 agtaaaagga ttagttacaa acaaggtagg cggtacaata ttacgatctt cagattcaat
      781 tattatttca tctttcttag gtctttcagt acttgcgatt tatcagaatt attactttat
      841 tatttctgcc ataatgagta tatttgcaat aatttatgaa tcatgtatag caggtatagg
      901 taacagtctt attgtagaaa gtcaagaaaa aaattatata gatttttgtg atattacaac
      961 aattataggt tggtttctgg caattagttg tgcatgcttt attacattat ttcagccgtt
     1021 catgattatc tgggtgggaa gtgataatct tatgagtatt tcatttgtta tatttatgat
     1081 agttcatttt ttcttatatg aaattgatca gttaattggg ttatacaaag atgctgctgg
     1141 aatatggtac gcagacaggt ttagaccatt aattacagca atgataaaca ttgtattaaa
     1201 tatcatcctc gttaattggt tcggattata tggagttttg gcggcaacca ttgtttcagt
     1261 attgttggtt gatttaccct ggttattata taatgttttc caaaatatat ttaaatttga
     1321 atcggcaggt aaatatgttt ggaagctcat aagtttgtgt gtaatagctt ttgttgcttc
     1381 ttatttaagt tattttgctt gttcgtatgt taatatcgga aatttcaaag tgataagttt
     1441 agtgataaat ttagttatat cggttgtcat atcgatttta gttattcttc tgtttaatat
     1501 ccgtaataag aagtttaagt atattttgaa agttcttacg tcggtattgg taaaaagatg
     1561 agaagagaca tcagaatgat atataaagta actacaattt ttaggctgcc gaataggcga
     1621 acgatgtata gtttttgttc caacgaagac tcttgttgaa attggagatg atatgaagga
     1681 gttacaattt tgactcatgt ttatgattga tcggttttaa aagtagtatg gagaagcttt
     1741 gggatcatcg gagaggatca aaataggtaa taatgttttt ataggaatga agtctagaat
     1801 actaaagggt gtacatatag gaaataatgt agttataggt gcaaatagtt tagtaaacaa
     1861 ggacgtatca gacgattgtg ttgttgcagg aaatctagca aaagtaatta tgtccctaga
     1921 tgaatattat aaaaagataa acaatatgaa gaatccgtaa aacttgtgaa actataccga
     1981 gaataatatg ggaaaaatat tgatgaaaaa gcattatatg agtttttagg gctgtttaca
     2041 gatggagatg acaatttgtc gaagagttgg gagcaatgca aaatttagta ggggattata
     2101 aacagactga caaggtattt aaagaacatg cacaagtatt tcataatatt gaagaatttt
     2161 tgaatactgt gagataggag ttgctacatt gagaaaagta tgtatttttc caatgtaaaa
     2221 gattacgagt ttgaaaacga caccataata ttatttttta agaagatatt atagagctta
     2281 gg
//
LOCUS       DAZKOM010000182         2272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000182 DAZKOM010000000
VERSION     DAZKOM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.190"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..>2272
                     /gene="acm"
                     /locus_tag="ACG8NF_002884"
     CDS             129..>2272
                     /gene="acm"
                     /locus_tag="ACG8NF_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0473276.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIF"
BASE COUNT          888 a          349 c          448 g          587 t
ORIGIN      
        1 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
       61 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
      121 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
      181 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
      241 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
      301 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
      361 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
      421 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
      481 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
      541 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
      601 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
      661 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
      721 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
      781 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
      841 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
      901 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
      961 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
     1021 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
     1081 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
     1141 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
     1201 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
     1261 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
     1321 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
     1381 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
     1441 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
     1501 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
     1561 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
     1621 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
     1681 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
     1741 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
     1801 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
     1861 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
     1921 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
     1981 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
     2041 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
     2101 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
     2161 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
     2221 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc tt
//
LOCUS       DAZKOM010000183         2266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000183 DAZKOM010000000
VERSION     DAZKOM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.191"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8NF_002885"
     CDS             <1..1125
                     /locus_tag="ACG8NF_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0473277.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACG8NF_002886"
     CDS             1474..2106
                     /locus_tag="ACG8NF_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0473278.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          845 a          308 c          454 g          659 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaaca
//
LOCUS       DAZKOM010000184         2254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000184 DAZKOM010000000
VERSION     DAZKOM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.192"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..>2254)
                     /locus_tag="ACG8NF_002887"
     CDS             complement(148..>2254)
                     /locus_tag="ACG8NF_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0473279.1"
                     /translation="KIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLGYL
                     QIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTAKD
                     LLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQYSA
                     MVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASELP
                     GVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYLEK
                     QYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKRNY
                     QTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDPGS
                     VVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELSSN
                     TYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESPGI
                     SRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQPHL
                     VKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAKPL
                     ASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQDHI
                     NLTIAKEIMDAYYDMFMAK"
BASE COUNT          572 a          504 c          411 g          767 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttt
//
LOCUS       DAZKOM010000185         2227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000185 DAZKOM010000000
VERSION     DAZKOM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.193"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..280)
                     /locus_tag="ACG8NF_002888"
     CDS             complement(<1..280)
                     /locus_tag="ACG8NF_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0473280.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="ACG8NF_002889"
                     /pseudo
     CDS             742..2124
                     /locus_tag="ACG8NF_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DAZKOM010000186         2218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000186 DAZKOM010000000
VERSION     DAZKOM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.194"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG8NF_002890"
     CDS             complement(51..1598)
                     /locus_tag="ACG8NF_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0473281.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8NF_002891"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8NF_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0473282.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          622 a          464 c          397 g          735 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaa
//
LOCUS       DAZKOM010000187         2132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000187 DAZKOM010000000
VERSION     DAZKOM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.195"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8NF_002892"
     CDS             complement(<1..914)
                     /locus_tag="ACG8NF_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0473283.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8NF_002893"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8NF_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0473284.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          723 a          376 c          412 g          621 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aa
//
LOCUS       DAZKOM010000188         2121 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000188 DAZKOM010000000
VERSION     DAZKOM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.196"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..1012)
                     /gene="cysK"
                     /locus_tag="ACG8NF_002894"
     CDS             complement(86..1012)
                     /gene="cysK"
                     /locus_tag="ACG8NF_002894"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0473285.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1152..2039)
                     /locus_tag="ACG8NF_002895"
     CDS             complement(1152..2039)
                     /locus_tag="ACG8NF_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0473286.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          586 a          474 c          356 g          705 t
ORIGIN      
        1 aatttttctc atcataaatt tttgtgtgtt cttttcatct ttaaaagttt tttacataaa
       61 cctatctgga gaaaaaccaa gtgccttata tctcttggta aagggctgta gataaataac
      121 gttcaccatt gtcgggaacc acagctaata ccttcttgcc ttttcctaat tcttttgcga
      181 cttttagcgc ggcagcaatt gcagcgccag atgagatgcc tactaaaatc ccttcttttc
      241 gcccaacttc tctagctgtt tccatcgctt cgtctccact aatagaaagg actttatcat
      301 acacagaagt atccagtgtt ttaggaacaa aacctgtacc gattccttgg attttgtgag
      361 gtcccgcttc tttcccttct aaaatagcag attctgctgg ttctaccccg attaattcga
      421 ttttcggata cacacgtttt aattctcggc cagcgccagt gatcgttcct cctgtaccga
      481 tacctgcaac aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc tcaggaccag
      541 tcgtcttctc atgcacaaga ggatttgctt cattttcaaa ttgtaatggt aaaaagtagc
      601 cgttttcttt tgccaaacgt tctgcttctc tgattgatcc agaaatacca tccgcaccag
      661 gagtcaaaat caactctgcg ccgtaccctt tcatcaataa acgtctttcg atactcatcg
      721 tctcaggcat cacgatgatt actttatatc cttttgcaac tcctaccatc gacaatccga
      781 ttcctgtatt tccagaagta ggttcaatga tagtatcacc tggtttcagt agaccatcgt
      841 gttctgcttt ttctatcata cttaaagcaa tacgatcttt tacacttcct cctggattaa
      901 aaaactcaag ctttacaaaa acatctgctg aatcttctgg tacgatttta tttaatttga
      961 cgattggcgt tttgccaatc aattcggtga ctgagttata gatagctgtc atcttcaatt
     1021 cctccttgtc tttgctcttc ttacttatca taatacaatc tgttcattta tggcgagtga
     1081 ttcgtctttg actagcaaaa aaaagacaaa gatataccat atatggtaat ttctttgcct
     1141 ttttatctaa atcaaacgtt tagttcttga ccaactaata aagaataact ggacatagac
     1201 atgccatttt tcgccattag atcatcaaca gacatgccaa atttttgtgc aatatcccat
     1261 agcgtatcac ctgacacgac tgtatatgtc tgaccattat ttgtatttac atctgttgtt
     1321 gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt ggttgttgta
     1381 gccgaacctg ttgatggcgt atcaaaacgg gttaaattat aagtattaat caatgagttc
     1441 aatttggccc cataatttgg atcggtagca taacgacctg tcaagtacgc tgttgcatca
     1501 taaaaacttg ttgtattgct tttccacacg ccagcataat gataatcgcc cgtggaaaaa
     1561 ctggttgaac gaagaacatt cgcatggtct tggaaggatt cccaataaga agaatatttt
     1621 cggaatggct cattcatcgt tacccattgt ccgtttacat attctttggt tgccatataa
     1681 acgacctgtc cgttatagct tcctttcaca ccgaatagat tataatttgg cgcactagct
     1741 aatccggaag tgccatatgc actttccaac aacgcttgag cgatcatgac tgaagcatac
     1801 aagtcattac ttgcagcaac ttctgacgca gaccagccga tttgatcgat aaacgcttga
     1861 ggatcagcga ctgtttgaga tccttgttca tctgcatgaa catgttgcgg taccgctgta
     1921 accatcatcg gcccaactaa aagactcgtt ccaattaaag gagcaatcgc ttttttattg
     1981 atcaccattt ttcttttagg tctatggtgc cgggcactgc gggtcttcag ctcttgcatt
     2041 caaattcctc ctcaaaataa aaaaacttaa taaaaagttc tcttcttaaa acaaacttaa
     2101 ttaattaaat tatactttta a
//
LOCUS       DAZKOM010000189         2094 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000189 DAZKOM010000000
VERSION     DAZKOM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.197"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8NF_002896"
     CDS             278..820
                     /locus_tag="ACG8NF_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473287.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACG8NF_002897"
     CDS             976..1425
                     /locus_tag="ACG8NF_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0473288.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2094)
                     /locus_tag="ACG8NF_002898"
     CDS             complement(1455..>2094)
                     /locus_tag="ACG8NF_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0473289.1"
                     /translation="RRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVT
                     DLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHT
                     DRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQ
                     LRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          748 a          331 c          395 g          620 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgt
//
LOCUS       DAZKOM010000190         2044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000190 DAZKOM010000000
VERSION     DAZKOM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.198"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1056
                     /locus_tag="ACG8NF_002899"
     CDS             148..1056
                     /locus_tag="ACG8NF_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0473290.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1056..1673
                     /locus_tag="ACG8NF_002900"
     CDS             1056..1673
                     /locus_tag="ACG8NF_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473291.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1677..>2044
                     /locus_tag="ACG8NF_002901"
     CDS             1677..>2044
                     /locus_tag="ACG8NF_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473292.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          739 a          294 c          438 g          573 t
ORIGIN      
        1 gttatccgag gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt
       61 atgatttgtg gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc
      121 ataccaccaa aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca
      181 gaaccctata attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt
      241 atggaagcta caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat
      301 tttgaatcag ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct
      361 aaaaaaggga aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa
      421 acagccaatt tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc
      481 tattttgtca tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca
      541 attgaagatt taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg
      601 gactggatga atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt
      661 ttccaagatt ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag
      721 gatattttga agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt
      781 gtagatcttc aaggtgtacg ccatgaatcg atttctgaac gtttcctatc cgatctaaat
      841 tatttgtatc aaaaaatgaa agcagcactt ttcacgattg aatacggtga aattgaagtg
      901 actgatattc ttcaagacga tctcttttca gataatcacg aagtcgaaaa aattgaaaca
      961 gtagaatttc caattgaaga aggagctttg attattgcaa ctgttgatga tccaaaacag
     1021 aatgttttca ctcttgagaa agttggggtg atttgatggt taaagtaaag agaatgatgg
     1081 agactgatga atctggtgtt aaacgtcaat ttatgccaat tacacatgtt tctgcagttt
     1141 taggcttaga aaaaatgata tcaggacaat caaaagttct ttcggttaat ggtaaatatg
     1201 gtgcagtcat tttaacgaaa gctgatttag gattagagaa tgcaattact gaactacctt
     1261 atgcaagcga gacaagcgat ggtattctta ctgctgaaat gtttcaaaag atcgtaaatg
     1321 gagaaggtgg aacatatatt ttgccgattg ctactcctga acaattagga ggtataaaag
     1381 ttggggaact cttagaagtt actgaagaag gtgttttatc tgcaactaag caaacagact
     1441 tcaatttctc tgaagaatta aagggaaaat tagaatctct aaaaatatta aaagcaggtg
     1501 caaatatatc aattgcagaa gatggaacaa ttagttctac aggtggtagt gggactggtg
     1561 gtgttaatca gtcatatgta gatcaaaaat tccaagaagc tgtaaatcaa gcagaaaatt
     1621 acacaaacga aagaattcca aattttactt ttgaaaaaat cggggaggta tagaaaatga
     1681 cagatatcgt aaaaataaaa caaagcggtg tacaggttta tccacaaact cattggaatg
     1741 ctatagaagg caaaccaaca acggtgaagg gagataaagg cgatcctggc caagcagcaa
     1801 caattaccat aggaaccgtt tctagtggtt caaccgcttc tgttacgaat gtaggaacat
     1861 catctgcagc tagatttaat tttgttttac caaagggaga taaaggtgat cctggaataa
     1921 atgccacaac tacagctgta gctacaacta cagcaaatgg gttgatgtcg tccacagata
     1981 aaactaagtt ggatggaatt gcagctggag cacagaaaaa tccaggtaat gctacaacta
     2041 cgac
//
LOCUS       DAZKOM010000191         2020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000191 DAZKOM010000000
VERSION     DAZKOM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.199"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..571
                     /locus_tag="ACG8NF_002902"
     CDS             101..571
                     /locus_tag="ACG8NF_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0473293.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="ACG8NF_002903"
     CDS             793..1896
                     /locus_tag="ACG8NF_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0473294.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          328 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac
//
LOCUS       DAZKOM010000192         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000192 DAZKOM010000000
VERSION     DAZKOM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.200"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8NF_002904"
     CDS             <1..378
                     /locus_tag="ACG8NF_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0473295.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8NF_002905"
     CDS             426..1826
                     /locus_tag="ACG8NF_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0473296.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKOM010000193         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000193 DAZKOM010000000
VERSION     DAZKOM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.201"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8NF_002906"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8NF_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8NF_002907"
     CDS             complement(728..1744)
                     /locus_tag="ACG8NF_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0473297.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKOM010000194         1974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000194 DAZKOM010000000
VERSION     DAZKOM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.202"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1245
                     /locus_tag="ACG8NF_002908"
     CDS             76..1245
                     /locus_tag="ACG8NF_002908"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0473298.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1512..>1974)
                     /locus_tag="ACG8NF_002909"
     CDS             complement(1512..>1974)
                     /locus_tag="ACG8NF_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473299.1"
                     /translation="MVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQ
                     VTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGP
                     FVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          697 a          336 c          301 g          640 t
ORIGIN      
        1 ataaatgtta tcaagataca agctatttta gctttattct ttctgtaaaa aagaaaggag
       61 actaaataaa ctattatgaa aaaaggaaaa catgctaaaa aaagaaaata ttcattttta
      121 atgatcgttg tcgtgttgtt tagccttagt gcgttctcta caggaactat tcttggatgg
      181 caattttaca ataatgaaca ctctcagata gttgcccatg ctcaatcagc tcaaaaaaag
      241 ttgatactga ctaccaatga acaacagctc gttgaaaaaa tcgatcaact attaagaaaa
      301 aatgattata ttggctcaat ttacgtaaag aaaaataatc gcatcatcat ggaaaaaggc
      361 tatggctatg cgaataaact cacgcaacaa ccaaatgatc cttctcttta ttatcagatt
      421 ggttcggttc aaaaagcatt gactactctt ttgattttaa aacaagtcga gcgtgggcac
      481 ctttctttag atactaagct ggctcaattc tatccacaaa ttgctggcag ccagcaaatt
      541 actatccgtg acttgctgta tatgcgctca ggcttgcacc gaactgcttc tccaaccata
      601 ccgatgactg atgaagaagt cgttcaattt gcgattcagc acttaaaatt tacagattat
      661 cagacctctc attatgattc attgaacttt accatattga ctggaatttt gatcaaatta
      721 acaaatcaac cgtatgaaaa attattgacc gatgatatca tcgaaccttt aggattacag
      781 catacaaaat tttatgacca agtcaaaaat tcatccaatc atgcactatc ctatcaaatg
      841 tctaaagagg atgattactt taaaccactg acagaatctg aaacagagat ccataacgaa
      901 ttagggactg gtaatatcag tatgtccgtc tatgatttaa atacgctgtt tacacaagtt
      961 ttatctggaa aactagtttc caaagactta ctcttctcat tatggcgtga aaatgcgaat
     1021 ggacatccat atagtggtgg ggtatatagc ggagataatt atatcctgtc gcaaggaaat
     1081 atcgactctt ttgatacatc tgctatattt agcaaagatt cacaggatgc tatcatcatg
     1141 gaaagcaata taaaagctga caaaactatc aaactgccta ctgctgatct aagaaaccaa
     1201 atctatgacc tgatggaagg aactactaag ttggcaaata gctgatatga tgaaaagata
     1261 gcaaatacta cttctttgaa tagcatttgc ttaaaaaaag tactcgacta aacgtaggaa
     1321 agagtgtggg acaaatctaa aaatcaattt ttgctacaga ctttaattcc aaataaacag
     1381 aacagaaaga ggtgttcttc tttctgttct gtccaaatta ttctaagatt tctctttacc
     1441 aacattattt gacaaagggc ctattaaatc taaaatagta tctttagatt gctaaaatat
     1501 gtgctagcaa atcattcatg gcttgttctt atttttaatt ttatgcaata aaaaagaaga
     1561 acaattagcg tgattataga aataaataat gagattttag aaaaaaatgt gtatgaatac
     1621 ctgattgtta ctttatttat tccactttca ctttttactg taacaaaagg acctccttgt
     1681 tttagtactg tagcattttt tgaattaaca gtaaccgtta atcctttata ccggaataca
     1741 ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa atgtaatttc atctttattt
     1801 ggagaaaagc tactataaat aacttgacca tcttctgtaa cttgatggtt tttcaaaagc
     1861 aattgatctt ttttatagcc tataggtaca taatcaaaat tctcaaattc ttcaagttta
     1921 ttttccattt gttcattaga ataagttcct tgaaaattca tttctttcac catg
//
LOCUS       DAZKOM010000195         1859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000195 DAZKOM010000000
VERSION     DAZKOM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.203"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..735)
                     /locus_tag="ACG8NF_002910"
     CDS             complement(34..735)
                     /locus_tag="ACG8NF_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0473300.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="ACG8NF_002911"
     CDS             complement(751..1191)
                     /locus_tag="ACG8NF_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0473301.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="ACG8NF_002912"
     CDS             complement(1191..1766)
                     /locus_tag="ACG8NF_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0473302.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          521 a          400 c          288 g          650 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatat
//
LOCUS       DAZKOM010000196         1817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000196 DAZKOM010000000
VERSION     DAZKOM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.204"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACG8NF_002913"
     CDS             <1..233
                     /locus_tag="ACG8NF_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473303.1"
                     /translation="GDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIFMIVIWFLNE
                     RSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(485..1057)
                     /locus_tag="ACG8NF_002914"
     CDS             complement(485..1057)
                     /locus_tag="ACG8NF_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473304.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACG8NF_002915"
     CDS             complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACG8NF_002915"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0473305.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          535 a          367 c          270 g          645 t
ORIGIN      
        1 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
       61 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
      121 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
      181 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
      241 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
      301 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
      361 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
      421 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
      481 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
      541 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
      601 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
      661 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
      721 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
      781 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
      841 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
      901 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
      961 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
     1021 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
     1081 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
     1141 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
     1201 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
     1261 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
     1321 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
     1381 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
     1441 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
     1501 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
     1561 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
     1621 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
     1681 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
     1741 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
     1801 tctattttgc acagtct
//
LOCUS       DAZKOM010000197         1733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000197 DAZKOM010000000
VERSION     DAZKOM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.205"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..557
                     /locus_tag="ACG8NF_002916"
     CDS             201..557
                     /locus_tag="ACG8NF_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0473306.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            567..1466
                     /locus_tag="ACG8NF_002917"
     CDS             567..1466
                     /locus_tag="ACG8NF_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0473307.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          585 a          297 c          353 g          498 t
ORIGIN      
        1 aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag
       61 ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat
      121 aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc
      181 tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga
      241 agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat
      301 ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa
      361 aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa
      421 tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt gctttagaac
      481 caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag
      541 ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg
      601 tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc
      661 acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag
      721 ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg
      781 agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa
      841 atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg
      901 actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt
      961 ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg
     1021 cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca
     1081 ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga
     1141 acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa
     1201 atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc
     1261 acacgattta cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt
     1321 ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc
     1381 gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac
     1441 aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt
     1501 gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt
     1561 tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta
     1621 gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt
     1681 tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcgag agt
//
LOCUS       DAZKOM010000198         1726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000198 DAZKOM010000000
VERSION     DAZKOM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.206"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1119)
                     /locus_tag="ACG8NF_002918"
     CDS             complement(100..1119)
                     /locus_tag="ACG8NF_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0473308.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="ACG8NF_002919"
     CDS             complement(1116..1340)
                     /locus_tag="ACG8NF_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0473309.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="ACG8NF_002920"
     CDS             complement(1337..1630)
                     /locus_tag="ACG8NF_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473310.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          484 a          354 c          291 g          597 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataa
//
LOCUS       DAZKOM010000199         1711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000199 DAZKOM010000000
VERSION     DAZKOM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.207"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..427)
                     /locus_tag="ACG8NF_002921"
     CDS             complement(<1..427)
                     /locus_tag="ACG8NF_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0473311.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLK"
     gene            complement(424..1077)
                     /locus_tag="ACG8NF_002922"
     CDS             complement(424..1077)
                     /locus_tag="ACG8NF_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0473312.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1365..>1711
                     /locus_tag="ACG8NF_002923"
     CDS             1365..>1711
                     /locus_tag="ACG8NF_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0473313.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
BASE COUNT          546 a          294 c          197 g          674 t
ORIGIN      
        1 atttaaggct taaatctttc gcatcttttt gcatatcagc tgtagaaaca aattgtagat
       61 ttgttttttt accatcatta agctttattt ctctttcttc aattatcaaa gaattactat
      121 cactttctaa attaacattt atattatctt tgatttgtat ttcattctta ttattttttt
      181 cttttatgaa tacttttatt tcactactct tggaataaaa ctccaaagtt tgttctatat
      241 attttatttc ttttccattc atattactag aaatttcatt tgccatatta tgaatctttt
      301 cttttctcgt ctccaaatat gttttaggaa agataaaaaa aattaatgaa tgaactaata
      361 taattattat tccaagaaca gaaaatattt gtatgaacat ttttggaaat atctttaatt
      421 ttttcatttt ctctccaatt tatatcctac atttcttata gtagttatac agtctaattg
      481 taattttttt ctaagttctt ttatatatac atctattact cgatcataag gagtttcttc
      541 gctatctttc catacataat caataatttg cattcttgtt aacacttgtc cattattatc
      601 caataaacat tttaatactt ctagttcttt tgcatttaca tctatttctt caccatttat
      661 tttagctgta tagctattaa aattaaccga tagatcctta tattcaaact tctctaaatg
      721 tccataattc ttcttaatta aagaatctat tctagctttt aagaccggca atgaaaatgg
      781 cttttctaca tatccgtcta ctaaattagt aaatgcatca attttatatt cttcatcact
      841 aaatgcagtc aagattagaa ttggtaaatt gcttttcttt ctaatttctt tcaacacttc
      901 taaaccattt ataaaaggta tctgaatgtc taagataacc aaatttatat cgctattaaa
      961 ttttgacaag gcttctcttc catctttagc ttgaataaca gtatatccaa attctgaaag
     1021 atattcactt attccctctc ttatcatatt atcatcttca acagttaata ttttcatata
     1081 tacctccact taagcctata cactagatat tatacagcat attatggctc tataatatct
     1141 agtgttggtg acaccttctg ttgccaatac tttttctttt ttctaataaa tgcaaacaga
     1201 caccttgaaa tcctttagaa taaagggttc tataatattt tgtgttgttg gaaaatcgaa
     1261 ggcgattttc tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta
     1321 aaatttagtc gaccaaaaca tcaccctacc aaaggagtcg ctctatggat aatcatacta
     1381 gaaaattact cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa
     1441 aagaatataa aggcgttcaa gcgcagttta ttaaagggag attggatgac tcaccttccc
     1501 attgtgaaca ctgtggatcg attcgtatta tccgcaacgg ttcctatacg acacgtactc
     1561 agatattgaa agtcaaagaa aagttgacta ttttagaatt aaaacgtacc cgttttctat
     1621 gctatgattg tgggcagacc ttttctgcga aaacggactt agttgatgaa catcatcaac
     1681 taacaaagga actgaagcag gcgattctca t
//
LOCUS       DAZKOM010000200         1596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000200 DAZKOM010000000
VERSION     DAZKOM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.208"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1552
                     /locus_tag="ACG8NF_002924"
     CDS             86..1552
                     /locus_tag="ACG8NF_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0473314.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          444 a          292 c          332 g          528 t
ORIGIN      
        1 tatttcttaa ataagttttt tagttaaatt tttcggtaaa gatgtataat attttctgaa
       61 aatttaaaga attggggtat agattatgga aaaacagcaa gacaagggct tcttcgggca
      121 gccgaaggga ttgtcaactt tgttcttcac tgagatgtgg gaacgattta gttattacgg
      181 tatgcgtgca ttacttatct attatatgta cgatacagtt gccaacgggg gacttggatt
      241 accacgagca acagcattag ctatcatgtc gatctacgga tctttgatct acatgtctag
      301 tatcgtcggc ggatgggtat ctgaccgagt gttaggagca aaacgcactg tattcttcgg
      361 tggagttttc attatgcttg gccacatcgt cttggcttta ccatttggtg taagtacatt
      421 gtttgtttcg atggcattga ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg
      481 catggtcgga catctttatt caaagactga cttgcgaaga gatgcaggat tttcgatttt
      541 ttacatgggt gtcaacttag gggcattatt agcaccacta atcgttggta caatcggtca
      601 agaatataac tatcatttag ggttctcttt agctgctatt gggatgttct ttggattatt
      661 acaatacatc atccaaggac gcaagacatt agacggagtg ggtacaacac cgccgaatcc
      721 tttgacaact gaagaacgta aaggattcat ccgtaacttg atcatcgcac ttgttgttat
      781 cgctgcagta ttcggcgggg cacaactaac tggtcatctg acaattgact tttttgtcaa
      841 tacaatcagt gtattgggga ttatcttacc gttgtattac tttatcaaaa tgctgacttc
      901 aaaagatgta actgctgaag aaaaaccaag agtatgggct tatatcccat tattccttgc
      961 agcaatcatt ttctggtcat tacaagaaca aggttcatct atcttagctt tatttgctag
     1021 tgaacgtaca caatctacgc tatttggtgt aacgatccaa gcaagctggt atcaatcatt
     1081 gaatccaatt ttcatcttta ttctgacacc agtattcgta acgatgtgga cgaaacttgg
     1141 tgaacgtcaa ccatctacag tagtgaaatt ctctcttgga cttttatttg caggattatc
     1201 gtacttgcta ttgatgttgc cgggtatgct ttatggtaca agtggaaaag taagtccatt
     1261 gtggttgttg ggaagtttct tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg
     1321 tttgtctgtg acaacaaaat tagcaccacg agcatttgaa tcacaaacaa tcgctatttg
     1381 gttcttagct gatgcttctt cacaagcaat caatgcacaa attgcacgct tctacacacc
     1441 gggaacagaa tctgcttact tcggtattat cggtgcagta gcaatcgtcg gtggtatctt
     1501 gctatttact gtgaaagaac caatcaaaaa actaatgggc tctattcatt aatcaatgaa
     1561 cataaaaaca ggaattgctt atttgcagca gttcct
//
LOCUS       DAZKOM010000201         1558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000201 DAZKOM010000000
VERSION     DAZKOM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.209"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8NF_002925"
     CDS             132..344
                     /locus_tag="ACG8NF_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473315.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG8NF_002926"
     CDS             506..1024
                     /locus_tag="ACG8NF_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0473316.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG8NF_002927"
     CDS             1031..1543
                     /locus_tag="ACG8NF_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473317.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          613 a          181 c          242 g          522 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaa
//
LOCUS       DAZKOM010000202         1541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000202 DAZKOM010000000
VERSION     DAZKOM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.210"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG8NF_002928"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG8NF_002928"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0473318.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          533 a          198 c          250 g          560 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc a
//
LOCUS       DAZKOM010000203         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000203 DAZKOM010000000
VERSION     DAZKOM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.211"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8NF_002929"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8NF_002929"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKOM010000204         1495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000204 DAZKOM010000000
VERSION     DAZKOM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.212"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..569)
                     /locus_tag="ACG8NF_002930"
     CDS             complement(270..569)
                     /locus_tag="ACG8NF_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0473319.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            920..>1495
                     /locus_tag="ACG8NF_002931"
     CDS             920..>1495
                     /locus_tag="ACG8NF_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0473320.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTL"
BASE COUNT          509 a          236 c          200 g          550 t
ORIGIN      
        1 tttcattttt tcaatctata tttccaattt ttaacttaca aaaatgcaac attaataaat
       61 aacgatagtt tcataaaata cacttttaat taacattatt agtttatact agctgttttt
      121 gtgttaatta aaggataaaa tacaatataa agaaacatct gtttctttat ttagaataaa
      181 aaaatacaga ctctgttcaa ataaatgaac ctagcctgca ttatgagatt gactatactt
      241 tttaattcat tgagatgaat aactcgtttt tataaatgcc caaaagtaat cggtgccgaa
      301 gccattcctc gacctaattc tttttcaaat tttcctattg ttgatatctc catataaaac
      361 ggtgatactg gtttagacaa ttctcccctg attgctaacg gacgtaaact acgttccaaa
      421 ttatttatga ctttaggata atactgacct ttttctaagc ctgtctttgc gtctatcaaa
      481 agtatacgtt ccttttcttt gatctctgga tttaaaacga gatcatataa ttttagtacc
      541 atgttttcta tttcttcact tttagacatc acgatttcct cccaaaaata tatttcttcc
      601 cactacattc tataaacaaa gtatacgcta catctagatt tttttaaatc attactggat
      661 tgttaaaacc tttcaataca ctgttatggc aacgattttt attttaaaaa cattctaaat
      721 taaatcagta aaaattttta ttctaagatt tttattagaa ttacgcctaa tttgtgtctc
      781 tcagggcaat tctaagaacg tttcaagaga tagcgacact atcgttatcg aaaaaggcga
      841 aaacctatct cattttctgc taatgtaagt tggtttagct tttgttaata tgactttatg
      901 aaaatggagg taattaaata tgatttttcc taaagttaat gaagcaagct tgaataaagg
      961 atcctttttt catatctatg aaaacgaaga ttttgaactg tataatatcc tttcacaaaa
     1021 agaggtcatc gaaaaccaaa taatctgcca tccaattatt tacaaatcta ttttcaagta
     1081 tgcatttttt gcttccaatg attttcaaag aatggagatt accgactgta tattcgaaaa
     1141 ctgtgatttt tcaggctgta tattgacaaa aagcatgttg catcgcgtga aatttgtcaa
     1201 ttgtaaatta acaggtacta aattgatgga aagttatatt gggaacacgt tattcgagaa
     1261 ttgtaaaatg gactatgtaa atttatctgg ttcaaacata aaagaaagta cctttgagca
     1321 ctctgtctta tcaagcgcag attttgtaga ctgttccctc actaaaataa tgttttctac
     1381 taatgattta gaaaatgcta gcttttttga tactgattta aaaaatatcg atttaagtag
     1441 caactccttt gaaatgattg aagtacgctc ggaacatcta aaaaattgta cgctt
//
LOCUS       DAZKOM010000205         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000205 DAZKOM010000000
VERSION     DAZKOM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.213"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8NF_002932"
     CDS             <1..828
                     /locus_tag="ACG8NF_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0473321.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="ACG8NF_002933"
     CDS             828..1448
                     /locus_tag="ACG8NF_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473322.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DAZKOM010000206         1458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000206 DAZKOM010000000
VERSION     DAZKOM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.214"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG8NF_002934"
     CDS             <1..354
                     /locus_tag="ACG8NF_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0473323.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="ACG8NF_002935"
     CDS             355..903
                     /locus_tag="ACG8NF_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473324.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1163..1458
                     /locus_tag="ACG8NF_002936"
                     /pseudo
     CDS             1163..1458
                     /locus_tag="ACG8NF_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          575 a          182 c          311 g          390 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg
      961 atttgcatga aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat
     1021 gagcatagga acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa
     1081 caagtgtatg ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata
     1141 aaactattag gaggagatgt aggtgaaaaa gtttaaaaag aaaatatatt taggattggt
     1201 tgttggggtg agtatgtttg ggttcatggc tcatactaaa caagtaactg cagatactaa
     1261 ccaaaaagaa aggattatga gccaattaga tgactctcaa ataagtatgt acccaatgag
     1321 ctaattgtca gtttaaaggc tacacaaaga atttcaacag agaaaaataa accgcaaaat
     1381 ttattgggtg taaaagttat ggtggaaaaa gatttgactc ttaggacaaa tgaaagcgat
     1441 tgtaataagc gagatcaa
//
LOCUS       DAZKOM010000207         1456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000207 DAZKOM010000000
VERSION     DAZKOM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.215"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1353
                     /locus_tag="ACG8NF_002937"
     CDS             121..1353
                     /locus_tag="ACG8NF_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0473325.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          478 a          276 c          312 g          390 t
ORIGIN      
        1 tgaaacttaa ccttaaaaaa ataacctcct ttttttattt aaagcctcat tgtttgaatc
       61 ataattaaat ggtatcatgc taatagttac cagaaaaaat ggtgaggagg aatttttatt
      121 atgttatctg attttaatga acttctaaaa aaatatgcac gcttaattgc agaaactggc
      181 gtagccacag aaaaaggaca cacagttgtt ttacagatca gcgtggaaca ggcacccctt
      241 gctcgattga tcacacaaga agcttataaa ctaggagccg ctgaggttat cgttcaatgg
      301 actgacgacc agatccaaag agaatttctt ttacatgctg caacggatcg tattgagaat
      361 gtaccacaat ctaaaatcga tcaagcggat gaatggcttg aaaaaggagc aagcaggatc
      421 agcgttgttt ctgccgatcc cgatgcattt gctggtgtag atagccatcg agtagcgtct
      481 tatcaatcag cgaatggcaa agcactaatg aatttacgca aggcaactca agcaaataaa
      541 gtcagttgga ctgtcgtggc tgcggctggt aaacaatggg cagcaaaagt cttcccagaa
      601 ctgccagaag aaaaacaagt tgaggcatta tgggatcaga ttttcaaaac gactcgtgtc
      661 tatgaagaag accctgtctt agcatggaag aagcatgatg aaaaattagc aaaaaaagca
      721 gaagaattga atcgagaaca attttctgcc cttcactata ctgctcccgg tacagatatc
      781 attatcggat tgcctaaaaa ccatctttgg gaaggcgctg gaagctataa tgctcgtgga
      841 gaaaaattca tggcaaatat gccgacggaa gaagtgttca cagcacctga cagtcatcga
      901 gtagatggtt acatttcaag tacaaagcca ctgagttatg ctggcaccat catctcgggt
      961 atgaaattta cttttaaaga tggaaaagtt gttgattttt ctgccgaaca aggagaagat
     1021 gtcttagcaa aactgcttga tacagatgaa ggtgcgcgtc gtttaggaga agtcgcactt
     1081 gtacctgacc cttcacctat ttctcaatct ggcatcatct ttttcaatac attatttgat
     1141 gaaaacgcat cgaatcactt agcactaggt tctgcttatg ccttcagtgt gaaaggcggt
     1201 acagaaatgt ccgatgaaga attagcagaa gctggattga atagaagtca aacccacgta
     1261 gatttcatgg taggatctga caagatggat atcgacggaa tccgtgaaga tggctctaca
     1321 gtaccgattt tccgaaatgg cgattgggct tgagtatact ttcgcacaca tgcaaaaaat
     1381 aatataaagc atagaataaa ggatctaagc tattggctta gatcctttat ttcgctaaac
     1441 aaccccaccg tttttt
//
LOCUS       DAZKOM010000208         1455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000208 DAZKOM010000000
VERSION     DAZKOM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.216"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG8NF_002938"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG8NF_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="ACG8NF_002939"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="ACG8NF_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0473326.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          426 a          259 c          237 g          533 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaa
//
LOCUS       DAZKOM010000209         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000209 DAZKOM010000000
VERSION     DAZKOM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.217"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8NF_002940"
     CDS             complement(150..1337)
                     /locus_tag="ACG8NF_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0473327.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKOM010000210         1400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000210 DAZKOM010000000
VERSION     DAZKOM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.218"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..425
                     /locus_tag="ACG8NF_002941"
     CDS             15..425
                     /locus_tag="ACG8NF_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0473328.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            439..795
                     /locus_tag="ACG8NF_002942"
     CDS             439..795
                     /locus_tag="ACG8NF_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0473329.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          514 a          209 c          263 g          414 t
ORIGIN      
        1 aaaggagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc tgtagaaaag
       61 caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac
      121 cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata
      181 ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt
      241 taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga
      301 ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc
      361 gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt
      421 catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt
      481 ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa
      541 gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca
      601 gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt
      661 gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct
      721 ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt
      781 atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt
      841 aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta
      901 ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt
      961 aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt tttaagtagc
     1021 aaagcaataa aatagtattt taaaaaacat ataaaatagt tgttgacatg ttagaaatga
     1081 catgataaga taactgagtt gttaaaaaag aaccacttgt gaagcttgat tctatcgaaa
     1141 aaaacttcaa aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg
     1201 taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct
     1261 ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcggggaa cactcctgat
     1321 gaagtagacc tttgaaaact gaacaaagta agacaaacca aatgtgtagg gcgtcttgat
     1381 tcaattcaag acaacaaaca
//
LOCUS       DAZKOM010000211         1388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000211 DAZKOM010000000
VERSION     DAZKOM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.219"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8NF_002943"
     CDS             154..417
                     /locus_tag="ACG8NF_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0473330.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="ACG8NF_002944"
     CDS             417..689
                     /locus_tag="ACG8NF_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0473331.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="ACG8NF_002945"
     CDS             766..1095
                     /locus_tag="ACG8NF_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0473332.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1085..1371
                     /locus_tag="ACG8NF_002946"
                     /pseudo
     CDS             1085..1371
                     /locus_tag="ACG8NF_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          525 a          169 c          277 g          417 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa acatgtcatt aagtatttaa aaagctaaaa
     1141 gagaagccat tgaaagaaaa atttttgaat ttaatttatg atgaaattgc gattgatcca
     1201 tataagggtg aacaaaaaac tggggattta tcaggtattt ggtctagtgg gttcaaatat
     1261 gcgggcacaa catatagagt agcttacgag attatagaaa acaaggttat tccagtattg
     1321 ttatgtggaa cgcatgaaaa cttttatgaa cagttgaaaa aattaatcta gtgatttgtt
     1381 gtcgttct
//
LOCUS       DAZKOM010000212         1386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000212 DAZKOM010000000
VERSION     DAZKOM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.220"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            379..711
                     /locus_tag="ACG8NF_002947"
     CDS             379..711
                     /locus_tag="ACG8NF_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0473333.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            621..815
                     /locus_tag="ACG8NF_002948"
     CDS             621..815
                     /locus_tag="ACG8NF_002948"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0473334.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            854..1000
                     /locus_tag="ACG8NF_002949"
     CDS             854..1000
                     /locus_tag="ACG8NF_002949"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473335.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1007..1162
                     /locus_tag="ACG8NF_002950"
     CDS             1007..1162
                     /locus_tag="ACG8NF_002950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0473336.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          487 a          245 c          244 g          410 t
ORIGIN      
        1 atgattttaa ttatcaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca
       61 gtctcactcc taaaattact ttatttacgt atgaagtggg caggaggtcg aatccagaac
      121 tgggctatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct
      181 tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa
      241 aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt
      301 gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac
      361 cggaaggaat gaactctatt gagaaaaaca tatgatcgta aatttaaact aaagatttca
      421 caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct
      481 cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct
      541 ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa
      601 cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga
      661 tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta
      721 tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag
      781 aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga taatttgaac agaaccaaat
      841 tgatgctgtt tgggtgatgg atattactta cattccatgt aatgacggtc gcctctattt
      901 gtccacctat attgacttag caactcgtat tctacgttac tatatggtag attcacacat
      961 gaaaaaagag attgtcgtcc gtccattaca aatttattaa ggagaattgc ctacggtaat
     1021 tcattccgat agaggtagcc agcatcggtc ttttatttat caagagctgt tgaacgacca
     1081 ccacatcaca cattcagtga gccaaactgg gactcctgtt gacaatgcag taattgaagc
     1141 gtttcatcga tcaatcaaat gagaattaat cgactccaac aaatataaaa gtaaaataga
     1201 gatgaaggtg ttaatccaag attatttaga agactactac ccaaacaaaa gaatccatac
     1261 aaaatttatg atgactcctt gacaatttga agcgaagaaa ataagggatc tataatatct
     1321 agtgttggtg acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga
     1381 cacctt
//
LOCUS       DAZKOM010000213         1363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000213 DAZKOM010000000
VERSION     DAZKOM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.221"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG8NF_002951"
     CDS             5..403
                     /locus_tag="ACG8NF_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0473337.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG8NF_002952"
     CDS             387..1082
                     /locus_tag="ACG8NF_002952"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0473338.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          520 a          203 c          273 g          367 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat att
//
LOCUS       DAZKOM010000214         1342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000214 DAZKOM010000000
VERSION     DAZKOM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.222"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8NF_002953"
     CDS             <1..459
                     /locus_tag="ACG8NF_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473339.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8NF_002954"
     CDS             473..904
                     /locus_tag="ACG8NF_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473340.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG8NF_002955"
     CDS             906..1043
                     /locus_tag="ACG8NF_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0473341.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            1081..>1342
                     /locus_tag="ACG8NF_002956"
     CDS             1081..>1342
                     /locus_tag="ACG8NF_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473342.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQ"
BASE COUNT          502 a          195 c          278 g          367 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa atcagatcga
     1261 ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca tacagaaaaa
     1321 ttggtggaac acacgcaaca aa
//
LOCUS       DAZKOM010000215         1340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000215 DAZKOM010000000
VERSION     DAZKOM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.223"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1101
                     /locus_tag="ACG8NF_002957"
     CDS             94..1101
                     /locus_tag="ACG8NF_002957"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0473343.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1113..>1340
                     /locus_tag="ACG8NF_002958"
     CDS             1113..>1340
                     /locus_tag="ACG8NF_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0473344.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLE"
BASE COUNT          531 a          166 c          250 g          393 t
ORIGIN      
        1 ttgacatcat tattgataat tagtttagtt atatattttt taaagattaa gaatcgaatt
       61 atagatgact ataattcata ggaggagata taagtgttta aaggaaaaac aatattagta
      121 actggcggaa ctggttccat tggaagtgaa attgttcgtc agcttttaaa atatgaacca
      181 aaagctatca gaatttatag tagaggtgaa gaaaaacaat tttatatgca acaagagctc
      241 tcaaatcaca ctaatttgag atttttaatt ggtgatatca gagactctca aaggattgat
      301 tatgcatgtc gtgatgtgga tattattttt catgctgccg caatgaaaca cgttccagca
      361 agtgaatata atcctatgga agctgtaaaa acaaatgtga tgggaactca aaatgtaata
      421 gatgcagcga tcaaaaataa tgtaaaacat tttgtaggta tcagtacaga taaagtagtg
      481 agtcctacta acaccatggg tgcgacaaag ttattgagtg aaaaattaat aatagctgcc
      541 aatcagtata aaggaaaaac agaaacaata ttttcttgtg taaggtttgg aaatgtcatg
      601 gggtcaagtg gatctgttat tcctcttttt agtaaacaaa taaaaaacaa taaagatatt
      661 actttgacag atgttgatat gacacgattt atgatgtcaa taccggatgc tgcaagatta
      721 gttctgtctg ctgctatgaa ttcaaaattt ggagaaacat atgtcttaaa aatgccggca
      781 atcaatattt taactttagc tcaatcatta ataaaaatat ataatgatac acatgatgaa
      841 aaatatagag gtgaaataaa agttgtaggt atcaggccag gagaaaaatt atatgaagag
      901 ctaatgacta cagacgaaat tgaacgctca tatgaaaatg ataaaatgta tatcattcca
      961 ccacactatc atgctactga cgaactgtac aaagattatg aaaaaattaa aatcactgca
     1021 tactcttcag atgcaacaga attaatgagt ggacaggaat ttattagaat cgcaaaagag
     1081 aataatgtcg tgacttggta aggagtttta tgatgaaatc agaagtatat gtaattgcag
     1141 aaattggtgt aaaccataat ggaagtctag atttagcttt agaaagtatt gataaagcta
     1201 aagaatgtgg agctaacgcg gttaaatttc aaacatttaa aacgaataag ctagtttcca
     1261 aaaaggctcc aatggcaaaa tatcagaagg ataatcttaa agaggacagt tctcaatttg
     1321 aaatgctaaa aaaattagaa
//
LOCUS       DAZKOM010000216         1323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000216 DAZKOM010000000
VERSION     DAZKOM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.224"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG8NF_002959"
     CDS             283..522
                     /locus_tag="ACG8NF_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473345.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG8NF_002960"
     CDS             complement(884..1144)
                     /locus_tag="ACG8NF_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473346.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          432 a          241 c          178 g          472 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 aga
//
LOCUS       DAZKOM010000217         1284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000217 DAZKOM010000000
VERSION     DAZKOM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.225"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>1284
                     /locus_tag="ACG8NF_002961"
     CDS             4..>1284
                     /locus_tag="ACG8NF_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0473347.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          431 a          249 c          246 g          358 t
ORIGIN      
        1 tatatgaatg attctatcaa aaaaatgctg agaataacag agaaagattt gatgattaca
       61 gaggtctctt acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg
      121 cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa cgggaaagca
      181 attatagtga aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata caatcatatg
      241 cctttggtta tgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg cacccattgg
      301 acggctcaaa gttattttgt ccaacccaga cattcaatcg caaatcatgt tagatataaa
      361 attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaag ctgtcaggta
      421 tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc aaagcaatct
      481 aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc ttccatagaa
      541 gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga tgttttgcct
      601 acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc agaagaagta
      661 gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg tgttctgcca
      721 aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca agcgttcaat
      781 gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag ccaggcagaa
      841 aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa
      901 tacaaaacat ggaaaagttt ccgagcacca aaatacccat ttcttactga agcaatgatg
      961 attgatcgat tgcttgagtt ttctccgccc ctaaaggagg cgtatccctt ttttcatgaa
     1021 ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt ggcagaactt
     1081 cccgaaacgt tagatgacag ctttcgggaa aagcttcaaa accttctgac ctatgaagaa
     1141 ggcatcacca acgcaatgat ctatccttat tccaatggaa aaatagaagc gaagaatacc
     1201 cacataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa catgagaatt
     1261 agaatctttt tgatcaatca atta
//
LOCUS       DAZKOM010000218         1280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000218 DAZKOM010000000
VERSION     DAZKOM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.226"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..885
                     /locus_tag="ACG8NF_002962"
     CDS             <1..885
                     /locus_tag="ACG8NF_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0473348.1"
                     /translation="VPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKI
                     IKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEE
                     KVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELE
                     VIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEF
                     DVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTF
                     TKLAKAEQYRRSLELSKD"
     gene            957..>1280
                     /locus_tag="ACG8NF_002963"
     CDS             957..>1280
                     /locus_tag="ACG8NF_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473349.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNV"
BASE COUNT          563 a          150 c          207 g          360 t
ORIGIN      
        1 gtccctctta ttttaaataa acgagagaaa aataatttaa agaaaaaaat gctcattcca
       61 tttgattcag aagtgaaagg attactcaat aaactaacta tttctaatat agactcgact
      121 agggaaaaaa tcataaaaat atttgataaa attaataatt tattcaagaa tgacaatgct
      181 gaattaagga agggtctttt agagtttaaa cttcaagaac tagaacttca ttactcttat
      241 ttagtgaaaa ttaccgaaga aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa
      301 gaggaaaaag ttcgtagaga aattgatcgt gaaaagaaac gtattgataa agaacaacgt
      361 caattcaatt ctgaaatttc caaattaatc gcatatatgc aaaaagctaa tgcagatgtg
      421 gaaaaagaat tatatgcaaa taaaattcaa gaactggaag aaaaattaaa agaattagaa
      481 gttatacgtg aaaatgtatt acaaagagaa ttaaatacta gagccggtta tgtgtatgta
      541 attagtaata ttgggtcttt tggtgaagac atatataaaa ttggtatgac tagaagatta
      601 gaacctatgg atagaataaa agaattgagt agtgcatcgg ttccatttga atttgacgtt
      661 catgcgatga ttttttcaga ggacgctccg tctttggaaa ctaaattgca taatcatttt
      721 agaaaacaag aagttaataa aattaatcaa agaaaagaat ttttcaaagt ttcactagac
      781 gaaatagaga aagttgtttt agaaaattat aatggaacag taacatttac aaaattagct
      841 aaagctgaac aatatagacg atctttagaa ttgtcaaaag attaaatata taaaaagcct
      901 tcgggctttt tcttttaaca attaatagga acgtatattc gaaaggaata gaaataatgt
      961 ggttagaaaa aataaatgac aaaaaattta agtacaccga acgttatacg gatcccttaa
     1021 ctgaaaaaaa gagaaaagtg tccgttactt tatctagcaa ttcacgccaa gcttggaatc
     1081 aagctaactt acttttgaat gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac
     1141 cactaacttt tggagaatta aaaactaaat gggatgaaaa atacaagcca actgtaaaag
     1201 aaagttccta tcgaacaact caggtttatc tatcattaat ttcaaaatat ataaaagatg
     1261 atgtacttgt aaaaaacgtt
//
LOCUS       DAZKOM010000219         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000219 DAZKOM010000000
VERSION     DAZKOM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.227"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8NF_002964"
     CDS             complement(31..1203)
                     /locus_tag="ACG8NF_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0473350.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DAZKOM010000220         1267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000220 DAZKOM010000000
VERSION     DAZKOM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.228"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..841
                     /locus_tag="ACG8NF_002965"
     CDS             <1..841
                     /locus_tag="ACG8NF_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0473351.1"
                     /translation="ESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNI
                     VMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVN
                     KRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLK
                     AKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLAD
                     TISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYS
                     RDH"
     gene            874..>1267
                     /locus_tag="ACG8NF_002966"
     CDS             874..>1267
                     /locus_tag="ACG8NF_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0473352.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKE"
BASE COUNT          493 a          136 c          231 g          407 t
ORIGIN      
        1 agaatctttt tctatagttc acaatcaggt ttattttggc gggggaatta atttatggtt
       61 agccaaaaag aatggaatca gacagagaat tgctcatagc catgcaactg aagatggaaa
      121 atctcaaaat attgtaatga atgtattgcg taaattttta accaaattgt tgttacaaaa
      181 tgcaacagac taccttgccg ttagtcaaga agctggtgaa agtctgtttc aaaaccaccc
      241 ctttgagata gttcataatg gtattgattt agagctttat agtaaaaatt cagaagctaa
      301 ggttaataag agaaaagaat tggatatctc aatgagtact tttgttgtgg gaaacattgg
      361 acgattagag aaacaaaaaa atcaatctta tttgttagag atttttttag aaatactaaa
      421 aatgaattct gattcttact tgattttagt aggtgaaggg tcacttagaa ataaactgaa
      481 agccaaagca gtagaattgg atatagagaa aagagtaaaa tttttaggag aaagaaacga
      541 tattcccgag ttattatctg tgatggatgt ttttgtaatg ccttctcttt atgaaggtct
      601 gccaatggtt ggattagaag cacaagcagc aaaattaaaa ttagtcttag cagatactat
      661 ttccaaagat acaaagttaa caaataatgt ggattttgta tcattagatg aaacaccaca
      721 agaatgggca aaaaaagtaa taaaagagcc gtatgctaat aaaataacac aagaactaaa
      781 gagttatgat gtttcgtaca ctactgaaca aatggagagt atatatagta gagatcatta
      841 aaaatgttca agatatttta ggagtgatta tttttgaaaa tatgttattt gatattggca
      901 tacaatgata ttggcaactt aaagagattg gtcaatcgat tgaacgaaaa tgcagttttt
      961 attattcata tagataaaaa aactgatata gaaccattta ttgaagcatt taaatcatat
     1021 gataatgttg aatttttatg taataaaaac cgtgttaaaa tttattgggg tggtttttcc
     1081 attgtacaag cagaaatcaa tttagtaaag agagcattac aaaatgagaa atatttaaaa
     1141 tatgtattat tatctggtgc tgattatcca atcaaagata atgagtatat ttataattat
     1201 tttaaaaaga atagttcagt ggagtttatt cgtggaatag atctagatca aattaaacat
     1261 aaagaat
//
LOCUS       DAZKOM010000221         1253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000221 DAZKOM010000000
VERSION     DAZKOM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.229"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            285..1100
                     /locus_tag="ACG8NF_002967"
     CDS             285..1100
                     /locus_tag="ACG8NF_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473353.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          482 a          172 c          203 g          396 t
ORIGIN      
        1 ataagaaatt caaaaaagaa aaaactttcc ttaaaaacaa aaaataggag tattcagttt
       61 cttaatagtt ttgtaatagt tgtatgtaga agttttctta atagtttttg taataattta
      121 ttaatcgtag tttatataac ttatttgtaa gtgactagag aattaaataa aaaataatta
      181 ctatctagta cgaaattaat tacataggat aatatattaa ggtgctggac tacaagtaaa
      241 aaatactgta tgatcatgac agattcaaga aaggagcaat aagattggtt aagatagcag
      301 gactttctaa aggtggaaaa accgtttcac aactttataa acaaaactac agagactgga
      361 gacagttcag aaaaaacatt aaggattctt actttattct accaacagaa cttgaaaaat
      421 atttaccgaa taacaaagct attaatttat atctgtatta ctgttttaag gcaaagaatg
      481 aaggcgggga ttcctggcat tcggtcaata ccatagcaga gtctctagga gtgacaacta
      541 aaactataaa cacctggaat aaaaccttag ttgatttagg tatgattgca cgtatagacg
      601 ataaccatct cagtaaatcg acaattttac ttcctctctc tagttactat aaattagccc
      661 ttgatagttc tttaaaggat gttaccgaga gcactgtcca tgaaattgat ggcaacttgg
      721 ttgcagttta tcatttgttt gaatggagaa aaaatgaggc tgatgaaaat tataacgttc
      781 cttataacac actctgttgt gtttttaaaa gaaaaacaca aaaagatact atctataaat
      841 ttatcttggt ccaagatgag aaaataagta atttcgaatt aagcactcct tctagtaaga
      901 tttatgacga tgcatataga tttgaatgtc aagatctaga agccctcctt tccgcaaata
      961 atctaaacaa tatagaacat gaaaactttg ttgtaaacac tagatttaat ttagtttctg
     1021 aaaaggactc agatttgtta gatttgctga tagagcttac taaaaatcta gataagaaag
     1081 aaaacattac tgtactataa ctagcatcgg tccatcatta agtttggact attagaacac
     1141 ttgctattac agaaaaaggt atgctagcca cgcttactat ttattggtat caaacaagta
     1201 tcttaatttg aaaggaagat gatttatggc taatttatat atagagaaca aag
//
LOCUS       DAZKOM010000222         1252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000222 DAZKOM010000000
VERSION     DAZKOM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.230"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>1252
                     /locus_tag="ACG8NF_002968"
     CDS             6..>1252
                     /locus_tag="ACG8NF_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473354.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRA"
BASE COUNT          514 a          203 c          277 g          258 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gc
//
LOCUS       DAZKOM010000223         1250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000223 DAZKOM010000000
VERSION     DAZKOM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.231"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..483)
                     /locus_tag="ACG8NF_002969"
     CDS             complement(112..483)
                     /locus_tag="ACG8NF_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473355.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(485..886)
                     /locus_tag="ACG8NF_002970"
     CDS             complement(485..886)
                     /locus_tag="ACG8NF_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473356.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(900..>1250)
                     /locus_tag="ACG8NF_002971"
     CDS             complement(900..>1250)
                     /locus_tag="ACG8NF_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473357.1"
                     /translation="TNVINKVINPALSINAPLIPTALSFSVTAVVSEIEDTEKIKIVE
                     IEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVKFNGTEIGR
                     QIFDIKVNKPVEKN"
BASE COUNT          400 a          223 c          166 g          461 t
ORIGIN      
        1 ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
      121 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
      181 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
      241 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
      301 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
      361 ttttttaaaa gcactgtagt atgctcttga aacagcagtt cttctataag attctttaga
      421 attatattta tcatctgatt caatttgttt ggaaatagat aaatattcat taaagtcaaa
      481 cattttattc ccccaaagtt gctactacag atttccccat atatttatag tcagataata
      541 cattatcgag agtatccaaa acatcgtaat ctgaggaaaa caattcagat gttgttttga
      601 taacaaagaa atacaagttt aaatcttcat catattttat attgatttca gcttttcttt
      661 ttaaaaccgt atttatcaca tcaaaaattt tgccatcatt acttgaaatt gtgttaatct
      721 cattttttgt agtaattaca agttgattat catttttagt tttcggagta gaacttgcat
      781 ccacaacaac tggagatgct agaattaccg atgcgcaagc aaaaatcgca gcaaaattta
      841 ttaagctacc actccgatgt gtatttgatg gtactatatc aagcatccgt atcgtctcct
      901 taattttttt ccactggttt gttgaccttg atatcaaaga tttgccttcc aatctcagtt
      961 ccattaaatt taacaacagc ataatgctct cctgcttctt caaccaagac atttctggcg
     1021 tttatattaa agtttatatc gttaacttga ggaggcaagc ttaccgatac ttcacccgta
     1081 gaaaaaattt gtttttcatt cttgtttaaa acttcaattt ctactatttt tatcttttct
     1141 gtatcctcta tctcagacac cacagctgtt acagaaaatg ataaagcagt aggaattaaa
     1201 ggcgcattaa tagaaagagc tggattaatt actttattaa taacattagt
//
LOCUS       DAZKOM010000224         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000224 DAZKOM010000000
VERSION     DAZKOM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.232"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8NF_002972"
     CDS             414..947
                     /locus_tag="ACG8NF_002972"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0473358.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKOM010000225         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000225 DAZKOM010000000
VERSION     DAZKOM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.233"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..300)
                     /locus_tag="ACG8NF_002973"
     CDS             complement(1..300)
                     /locus_tag="ACG8NF_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0473359.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(306..536)
                     /locus_tag="ACG8NF_002974"
     CDS             complement(306..536)
                     /locus_tag="ACG8NF_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473360.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(795..908)
                     /locus_tag="ACG8NF_002975"
                     /pseudo
     CDS             complement(795..908)
                     /locus_tag="ACG8NF_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          421 a          196 c          174 g          402 t
ORIGIN      
        1 ttaatctttg tatctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg
       61 atcgcttcga gcgtctagca catcagtcac gatatcaaac aaaaaatata cgtctttctt
      121 cattctagtt ttcgcagcag ggattttaaa gtagttctta ttagaatagt agagattaat
      181 taataagcta tcttcgattg ctaaaaagaa aacttctgaa tcccacacct tataaaaatc
      241 tttgacaaat ctattcgaag gatcaaattt aaaccataat tgcgtctcat taaaaagcat
      301 aaccattact ccgatctatt tttaaactta gtctctacct ctaatatata tcgagttttt
      361 attttgcctt cagagaatac tgtttctttt tttgcagtta caggttgttt attttcggaa
      421 aaagctaata tagctaaaat tgaaacatcc atctggaatt tgtctttttc gttgctttgc
      481 tcataaaaat ctgcatattc atcactaata ttttttctaa taaattcttc catcataaag
      541 atcacctcga aaagagtata cgaacaaacg ttctttttgt aaaggtggaa tttattatac
      601 taaataaata ggtgaacaag tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa
      661 taaaacacaa acaaaaagaa atcctattat gataacattt cttataataa caaacacaac
      721 agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca
      781 gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc
      841 gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt
      901 ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact
      961 aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg
     1021 gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg
     1081 aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat
     1141 agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa aaa
//
LOCUS       DAZKOM010000226         1184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000226 DAZKOM010000000
VERSION     DAZKOM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.234"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..494
                     /locus_tag="ACG8NF_002976"
                     /pseudo
     CDS             180..494
                     /locus_tag="ACG8NF_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(799..>1184)
                     /locus_tag="ACG8NF_002977"
     CDS             complement(799..>1184)
                     /locus_tag="ACG8NF_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0473361.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          428 a          164 c          214 g          378 t
ORIGIN      
        1 ataataatga agcttaatct tctttataga gaattgggct atttttaatg gcaaagaggg
       61 gaagaagatg tttcaaaaca aaggacaacg ctatgttgta aaaggagtaa aggaatctta
      121 ctggaatcaa aaatctttat tgagaaaatt atctttgaat ttaagcacaa agaaatgaag
      181 gtacactaga tagcaatgaa gatatctttg tcattaaacg tcgctatttt acacaagtaa
      241 gtgatgattt gattcatgtc ttttccaatg agtggtttgt ttctgagaaa aaattacatg
      301 cttctaatct gcaatatatg ccaggtgaag atccaattcc taatatgaaa gcaattatca
      361 acagtaagga ttacgaagga tataaagcca aacatccaga agcgaaacca tttaaatacc
      421 cacaagaaat gaagcgagca tggcgtaaaa tgttagatga tgagctgatt ccccttgaaa
      481 atgaattgag gtaaacgaga agaattcaat aaactggatg ataaaaatat aatatgacta
      541 taaattggtt acagcttcaa cctataagtt gtaataaatt agcctaaaag taatttatta
      601 atgatgaggt gaatttgatt ggtacaacaa gatatatcac ttagagaggg agaagcagca
      661 ctacaaacta cttttcaata agtaggaatt atagaaattt tactcaaaaa atcttcagta
      721 aacaatttgg ctaaataaga ttcagttgtt atacgagaag gttacggttc ttcttaacta
      781 agcttagtat gtttttattc aaggcatgat tttctctatg tgatcattca gtttctgtaa
      841 gagttctaat aattcttttt tggagtactt gtaattgctt tctacccacc gattgagaac
      901 ggatagacaa ccttggacgt ggtacgtagt aaagaatctc atttcttctg tgataatcgt
      961 tttcttgtct tcatatagcc atatgttcag atagttttct tcaataactt gtttaacttt
     1021 cttctgaaac gaaatatcac catggttgag gataaataag cgatataaaa aagtatttgc
     1081 gtaaacatag tcaatcaagc gagtgtatag ttcctcatag ctatgggctg gatcataaga
     1141 gagaatttca tttagatccc gaaaaaagtt gctttctaat tgat
//
LOCUS       DAZKOM010000227         1160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000227 DAZKOM010000000
VERSION     DAZKOM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.235"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..124
                     /locus_tag="ACG8NF_002978"
     CDS             <1..124
                     /locus_tag="ACG8NF_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0473362.1"
                     /translation="DYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            129..1076
                     /locus_tag="ACG8NF_002979"
     CDS             129..1076
                     /locus_tag="ACG8NF_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0473363.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          373 a          171 c          245 g          371 t
ORIGIN      
        1 agattatcta attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt
       61 tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag
      121 gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag
      181 ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag
      241 tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat
      301 ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag
      361 ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa
      421 gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt
      481 ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg
      541 tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag
      601 attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc
      661 caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata
      721 agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta
      781 gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag
      841 ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga
      901 ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt
      961 cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac
     1021 cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca
     1081 tactaagata agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt
     1141 gtaaaatcaa atctgggata
//
LOCUS       DAZKOM010000228         1133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000228 DAZKOM010000000
VERSION     DAZKOM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.237"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1005
                     /locus_tag="ACG8NF_002980"
                     /pseudo
     CDS             370..1005
                     /locus_tag="ACG8NF_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1020..>1133)
                     /locus_tag="ACG8NF_002981"
     CDS             complement(1020..>1133)
                     /locus_tag="ACG8NF_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0473364.1"
                     /translation="FISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          384 a          198 c          209 g          342 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta ccttatatta tttgtcaaat
     1021 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1081 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaa
//
LOCUS       DAZKOM010000229         1101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000229 DAZKOM010000000
VERSION     DAZKOM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.238"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>1101
                     /locus_tag="ACG8NF_002982"
     CDS             4..>1101
                     /locus_tag="ACG8NF_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473365.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFS"
BASE COUNT          348 a          151 c          149 g          453 t
ORIGIN      
        1 aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt tatagtttat
       61 gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat atttggcttt
      121 tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt tagctcccct
      181 attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta cccatggtta
      241 actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg ctataacctt
      301 tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt
      361 accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg
      421 agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct
      481 cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg
      541 gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat gactatggta
      601 atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga aatcttctta
      661 attattaaat taataaaagc tgctatagtt actttacttc tttcactagc gtttataggt
      721 cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga tatattacaa
      781 aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc agaattacta
      841 tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt taactttaaa
      901 aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat tctactttgt
      961 acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat tcaagctcct
     1021 tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct atttatttct
     1081 ccacagttgg aaaaattttc t
//
LOCUS       DAZKOM010000230         1101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000230 DAZKOM010000000
VERSION     DAZKOM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.239"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..224)
                     /locus_tag="ACG8NF_002983"
     tRNA            complement(151..224)
                     /locus_tag="ACG8NF_002983"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Ile,seq:cat)
     gene            complement(779..1093)
                     /locus_tag="ACG8NF_002984"
     CDS             complement(779..1093)
                     /locus_tag="ACG8NF_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473366.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          413 a          173 c          170 g          345 t
ORIGIN      
        1 agaagtataa ctatgtgaaa catcctttca ttaatccata aaaggataca taaaaaagcc
       61 actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaattct
      121 aacacagaac gattcaattg gctacattaa tgtaccctgt aggactcgaa cctacgaccg
      181 gacggttatg agccgtctgc tctgaccaac tgagctaagg gtagtggttg ttgccacata
      241 aagccataaa caatcaacca gtagaatgcg tggcaacaaa cctgttatcg catatcttgg
      301 agtgtgacta tttatgagtg atagtgaaga tatgcgtctc catatatgta tggttgagac
      361 agataaacat gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg tcaaggagag
      421 aaaagtagag aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa cattcaaaca
      481 tttagaaatc cccaatacgt attcaggaaa atctatttta aggtttacaa aaagaacttt
      541 tgttcgtaca ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa cacaattatg
      601 gtttaagcat gttccttcaa ataagttttt taattataaa ctctagagaa attaattcat
      661 cctaacataa aataattgtt cactcaaaag tttttctaga acaattaaaa cagcaggctt
      721 cacattcgct atacatttgt aagaagtaat aattcctgta ctaaaaggca taattgcttt
      781 aattattttt tgacttctct tcaaacacaa gaagtagttg tgtaataatt gcagaaataa
      841 ggattccaat aatagatata aaatttgaca tggaaacgtc tataacatta aaagaataaa
      901 gtgttgaaga aagtaaactg attgaaataa cccctccgta aaaaatagtc tgtgaatata
      961 cccacttttt ttcagaagac gtacttcgtt tggttctaaa acctatccct gcatttctac
     1021 tcttatcaaa aaaccaaata tttagaagta ataatattgc tattattaag ataaaattac
     1081 tgtaaaaatc caaaagctat c
//
LOCUS       DAZKOM010000231         1079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000231 DAZKOM010000000
VERSION     DAZKOM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.240"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG8NF_002985"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG8NF_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          378 a          196 c          219 g          286 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgca
//
LOCUS       DAZKOM010000232         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000232 DAZKOM010000000
VERSION     DAZKOM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.241"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8NF_002986"
     rRNA            <1..>1073
                     /locus_tag="ACG8NF_002986"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKOM010000233         1069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000233 DAZKOM010000000
VERSION     DAZKOM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.242"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8NF_002987"
     CDS             <1..969
                     /locus_tag="ACG8NF_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0473367.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZKOM010000234         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000234 DAZKOM010000000
VERSION     DAZKOM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.243"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8NF_002988"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8NF_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0473368.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DAZKOM010000235         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000235 DAZKOM010000000
VERSION     DAZKOM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.244"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8NF_002989"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8NF_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0473369.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZKOM010000236         1040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000236 DAZKOM010000000
VERSION     DAZKOM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.245"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..778
                     /locus_tag="ACG8NF_002990"
     CDS             86..778
                     /locus_tag="ACG8NF_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0473370.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          451 a          153 c          175 g          261 t
ORIGIN      
        1 catatgtaat ttaaaaacgc ttgacattgt gtttgagatc acttactttt agcatgtaaa
       61 agtattggat taaggagcta tgcaaatgaa aaagaaattt attactggtg ggattgtcgt
      121 tgcatttcta tgtctaattg gctacacagg cacgcggtac gcaaatgaaa ttattttgtg
      181 gggtggagag acagatattg atacaatcaa tgagaatctg acacagcttg atcatacatt
      241 gacagataaa gagcaagcga ttacagatct taccgctcga ctagcacaaa aagaacagac
      301 attgcaagat tcaaaggcag atgtccaaga ataccaaaca aaagtaaatg aattagaaaa
      361 ccaaaaaaat caatcggcag cagaaaaaca aaacttagaa agtcaattag tcagcaagga
      421 tgcacaacta caaagcaaac aaaatgaaat caatgtcaaa ctagatgaga tcaaccaaaa
      481 aaatgatgaa atcaatcatc tgaatgcaga ttggacagca agactacaag aagcacaagc
      541 aaaactttca gaattacaaa atactgtaaa tggtttgaat caacaaatcg caacactgac
      601 acaagaaaaa acaaatgtag aaactcagtt aacagataca caaaagaaat tagacgaagc
      661 aactgctgaa aactcaagct tgaaacaata tattcaaaag ttagaaaaag caaaagatga
      721 agtggaagat acagctgata aatctcagca aattgttgaa gaacatttgc gtaaataatc
      781 tttcttaaca agtaaaccta aattagtaaa gaagcaacag taatctatac taagtagttc
      841 agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac
      901 ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat
      961 gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca
     1021 actaacgggc aggataatga
//
LOCUS       DAZKOM010000237         1039 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000237 DAZKOM010000000
VERSION     DAZKOM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.246"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..254)
                     /locus_tag="ACG8NF_002991"
     CDS             complement(<1..254)
                     /locus_tag="ACG8NF_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473371.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSD"
     gene            complement(256..>1039)
                     /locus_tag="ACG8NF_002992"
     CDS             complement(256..>1039)
                     /locus_tag="ACG8NF_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0473372.1"
                     /translation="VQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPV
                     KIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVIL
                     QHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNE
                     LKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIF
                     ILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          395 a          178 c          127 g          339 t
ORIGIN      
        1 tcatccgaga aaaattgatt atggttagtt aacaagttaa ttttacgttt aaaggaagaa
       61 ccgattttcc ggtgattcac aaaaacaaca tcattaaata gccctaattc ttttatacgc
      121 tcactatatc ttctagcgtt ttcaaattca gaataaataa ataaatctcc ttttgattct
      181 cggtaaaaat tttgataaac atttaatata ttaatcaaat gtaaaggagt ccaagcaatg
      241 aaagctgtct tcatattaat tctccttttt taaatacaag taagaaatgc ttcccaaata
      301 ataccagaaa acaattgaaa tgacatcgcg cttattgaaa agtacatgag cctccaccat
      361 ttcatctata aaaatagata gtataaaaat taaaacaata ccaagaaacg cataattcac
      421 tttacttcta aaaaatgttt tgagtaaact aaagatattt aacacataga aagcatagat
      481 acatataaaa gcaattatac cacttgatac aagtacttga agaaatccat tatgcatgtt
      541 tccatgtgct ctttttagtt cattcacatc tgtagtatca agatctactt ttttcaaaaa
      601 ttttgcatcg ctattcccat agaaatcggt ctctccataa ccaaataaca aagttggtct
      661 tataacttta agcgcagctt tccaaatcgt aaaacgacca gctgaaattt cggagttatc
      721 actagaatgc tgtaatatta cttctttttc tggaagtgga gttttatctg attcatttga
      781 ctctataact tctacttttc tatcaatcga tacaggaatt gttgctaaaa aatccttagc
      841 ataattagta ccaaaagaaa aatataccaa tagaactaat aatatgctta ccatccattt
      901 gatttttaca ggtagtactt ctctaatgct ctttctaaat tttgaattgg caaacaacaa
      961 aagaaataat actaaaaaag aacaaattac tacctgggca cctctagacg aagaaagtgt
     1021 aatataaaat atttgtacg
//
LOCUS       DAZKOM010000238         1007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000238 DAZKOM010000000
VERSION     DAZKOM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.247"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..708
                     /locus_tag="ACG8NF_002993"
     CDS             <1..708
                     /locus_tag="ACG8NF_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473373.1"
                     /translation="FTGEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKEL
                     QAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFI
                     QTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDT
                     GRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAK
                     RKNEIYYEQQLSQKERV"
     gene            769..>1007
                     /locus_tag="ACG8NF_002994"
     CDS             769..>1007
                     /locus_tag="ACG8NF_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473374.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDS"
BASE COUNT          380 a          156 c          216 g          255 t
ORIGIN      
        1 tttactggcg aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct
       61 acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca
      121 actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac
      181 acttcggaga aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat
      241 gataaggaag cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa
      301 ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa
      361 ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagagaa taagaatatt
      421 gtgcccgttg gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg
      481 ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg
      541 attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt
      601 gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa
      661 aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa
      721 agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa
      781 aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat
      841 ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt
      901 tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt
      961 gaattagcta aacttttttt catagacaaa gagatccaag actctaa
//
LOCUS       DAZKOM010000239          916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000239 DAZKOM010000000
VERSION     DAZKOM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.248"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..526
                     /locus_tag="ACG8NF_002995"
     CDS             <1..526
                     /locus_tag="ACG8NF_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473375.1"
                     /translation="NSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFA
                     KVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTI
                     SFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPE
                     NWAELRKLVEYGII"
     gene            513..>916
                     /locus_tag="ACG8NF_002996"
     CDS             513..>916
                     /locus_tag="ACG8NF_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0473376.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFV"
BASE COUNT          311 a          121 c          136 g          348 t
ORIGIN      
        1 aaacagtaaa ttgatgaaaa acatacaatc aatctttggg atagaagaag tttttgtcgg
       61 agataaatgt atcttttttg atcaaccttt attatctgat ggttatggca tagatgaaac
      121 agatattttc gctaaagtaa atagtatatt ttctggaaaa aaagaaactt taattaagct
      181 tcatcctaga gcaaggacaa atcgttatgg tgaaaaaaac atattgaatt ccagtttacc
      241 gtttgaagtc ttgctacttg aaaatgactt ctcttcaaca cttttgatta gtcctatatc
      301 tacaatttct ttttcaccat ctatgatgtt tgaaaagacg atcaactcta ttatgttggc
      361 taaattaatt ttaaatgaat atacagcatt atctgaagat aaaaaagaaa cattgacaaa
      421 tattgttctt ttctgtgaac attataaccg tctatcagaa gaaaaaattt tactcccaga
      481 aaactgggca gaattaagaa agttggtaga gtatgggatt atttaacaca ctttccaagt
      541 caaaattagt taagtccagt ttctggtata ctatagggaa tttttttatt aagggaattt
      601 cattcataac tattcctatt tttgtaaata ttactactca agcagaatat ggtttgatta
      661 ataatttcac ttcatttgta tcaatttttt cgattcttgt tgggctaagc ctaaattcgg
      721 caattaataa tgcaagtttt gactataaag atgacataaa tgaatttatg tcatcggtac
      781 tttctctagc tacactttcg ttaattgtgt tcttattaat agggaacata tattttttgt
      841 tttcaaatgc tttttttgat atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt
      901 ttgctagttt tgtaat
//
LOCUS       DAZKOM010000240          909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000240 DAZKOM010000000
VERSION     DAZKOM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.249"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8NF_002997"
     CDS             <1..878
                     /locus_tag="ACG8NF_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0473377.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          291 a          202 c          184 g          232 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaat
//
LOCUS       DAZKOM010000241          904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000241 DAZKOM010000000
VERSION     DAZKOM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.250"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>904
                     /locus_tag="ACG8NF_002998"
     CDS             <1..>904
                     /locus_tag="ACG8NF_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0473378.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGP"
BASE COUNT          272 a          184 c          181 g          267 t
ORIGIN      
        1 gtaagtcttg ccaaagaaca ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca
       61 ttagtcaaaa agttcgttgg tctgtcattg ctcgcttaaa agagaatact tccatgacgg
      121 aaatcgctcg tcagaaaaat atttccactt cttcggtcta tcgtgtcatg aagcgattct
      181 accgcccatt gaatcctttc aaacagactt tgccaaaggt cttatgcttt gatgaattca
      241 aatcggttcg aagtgttgcc agtgcaatga gttttatcat gatggacgga caaagccatg
      301 cattattgga tattgtcgaa aatcgacgct tgccttattt agaacgctat ttttctcggt
      361 tttcattagc gactcgtgaa gccgttcaat tgattgttat tgatatgtat gcgccttatg
      421 tttcgttggt gaaaaagctt ttcccgaatg ctcagttgat catcgatcgt ttccacatcg
      481 tccaacatat tggcagaact ttcttgaatc atcgagtgaa agaaaccacg gtacgactta
      541 aaggaaattc ccactctcaa cgagggttag ggaaaaaatt gaagcgctat tggaaactcc
      601 tacaaaaaga agaagcaaag ttaaactatg aaaaacgggt ttggcgtgcc agttttaaag
      661 attatttgac tgaaagtgag attgccgatc gactgttagc tgcttgtccc aacttacgcc
      721 aaggctatca gttataccaa gacattcttt atgcggtaaa gaaaagagat cagtcactat
      781 ttgaggattg tttaactaga gacattagcg gtctgccaga aacctataca accactttac
      841 gaacgtttaa aaagtttctg ccacagatcc aaaatgcgct tcactacagc tattcaaacg
      901 gacc
//
LOCUS       DAZKOM010000242          902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000242 DAZKOM010000000
VERSION     DAZKOM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.251"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..858
                     /locus_tag="ACG8NF_002999"
     CDS             <1..858
                     /locus_tag="ACG8NF_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFQ0473379.1"
                     /translation="SAIISLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVG
                     ELKQQINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILEN
                     MKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDIL
                     KKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVND
                     WLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEF
                     FIFKIKIFS"
BASE COUNT          404 a           63 c          123 g          312 t
ORIGIN      
        1 tctgctatta tttctttaat ttatgcaaaa tcaataaaaa ataatataca agaaataaaa
       61 attgttactg ataaaatgat gaaacttgat aaaaaaatga gtttaaaagt tagctctaat
      121 gatgaagttg gagaattaaa acaacaaatt aatgatttgt attctacttt attaagaaca
      181 attgacgatt tggaatttaa aaataaagaa attttaaaat tagaaaagtt aaaatatgac
      241 ttttttaaag gtgcatccca tgaacttaaa acccctcttg ctagtcttaa aataatactg
      301 gaaaacatga aatacaatat tggaaaatat aaagaaagag atatttatat taatgaatgt
      361 attgaaatcg ttgatagttt aacaaaaaat atttctcaaa tattatcagt tcattctata
      421 gaaaatttga ataatgatga agaatatttg aaaataaatg atacattaga agacatatta
      481 aaaaagtatg aaatattagc aaatcaaaag aaaataactg tcaataatta tatattagac
      541 gagaatgttt atataggaaa aacagcttta aagattattc tatctaattt gattagtaat
      601 gcggtaaaat atactgatgt aaatggggta attaatattg ggatagttaa tgattggtta
      661 tatatagaga attcatacgg taacaataaa atttcgaata tggataaaat atttgatgtt
      721 aaatttgatt taaataagga aaatagtaat ggattaggtt tatatattgt aagtaatatt
      781 ctgaataatt acaatataga atataaagta ttgcaggatg aagaattttt tatctttaaa
      841 ataaaaatat tttcttaatt aagatataat tttgcaatag ttattgaaaa atagagactt
      901 at
//
LOCUS       DAZKOM010000243          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000243 DAZKOM010000000
VERSION     DAZKOM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.252"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..875
                     /locus_tag="ACG8NF_003000"
     CDS             252..875
                     /locus_tag="ACG8NF_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473380.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aaga
//
LOCUS       DAZKOM010000244          888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000244 DAZKOM010000000
VERSION     DAZKOM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.253"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8NF_003001"
     CDS             136..309
                     /locus_tag="ACG8NF_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473381.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          298 a          144 c          107 g          339 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaata
//
LOCUS       DAZKOM010000245          875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000245 DAZKOM010000000
VERSION     DAZKOM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.254"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>875
                     /locus_tag="ACG8NF_003002"
     CDS             <1..>875
                     /locus_tag="ACG8NF_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0473382.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRY"
BASE COUNT          303 a          167 c          161 g          244 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatac
//
LOCUS       DAZKOM010000246          870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000246 DAZKOM010000000
VERSION     DAZKOM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.255"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..781
                     /locus_tag="ACG8NF_003003"
     CDS             143..781
                     /locus_tag="ACG8NF_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0473383.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          289 a          137 c          132 g          312 t
ORIGIN      
        1 tacaaataaa agtagtataa tcaaaatgaa tatataagat aaatttttag caatacaaat
       61 atccataaaa aaacattgcc tattttaact cttcatgtat actgactcta tcttcaaaaa
      121 ctgaatagta ggagttatta tcatgaataa cattttagga aaaaaaatag aacttaaaaa
      181 agggatattg ggcactattg tcgttgtact cggtttgtta gggttgctat ttgcgccatc
      241 cattattagc attttttctt taactatcac tggatttata gcatttacac ttgtttttgg
      301 taaaaaagat acgaaaaaaa tgttctcgaa acctgtcgct cctgtcaaaa atatagccaa
      361 atattttttt atcaatgtag taatcagtgc cgcagtctcg gtatttttgc aacaaatttt
      421 gaaatggggt ttaacaggca atccaataaa tgaggctttc agcccgttat tatttattat
      481 tttgcctatc atgatacttg gagaagaatt gttctcagtg tattttctcg ctattttttc
      541 tagtaagttc agcattcctg tcgctagttt cttgtcagca atcatctttg gatttatcca
      601 ttacagtact tatgataatg gaaatatttt acacactgta gcacacatcc ttttgataca
      661 aggggtggca agattgttat tcaaccaagc tgccattaaa agcaactcga tcattacaag
      721 ttgggcagtt catgtgatat ttgatttcac agctatattg ctagtcgttt tattctccta
      781 aatttttata gaaataatac atgctctatt gctaagaaag catactttat ttcctttaaa
      841 caaaaagaca gatgtatcaa tggattaaaa
//
LOCUS       DAZKOM010000247          845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000247 DAZKOM010000000
VERSION     DAZKOM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.256"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..795
                     /locus_tag="ACG8NF_003004"
     CDS             <1..795
                     /locus_tag="ACG8NF_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0473384.1"
                     /translation="HQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER
                     PVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFN
                     SEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKN
                     FFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMD
                     FRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          306 a          154 c          146 g          239 t
ORIGIN      
        1 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
       61 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      121 cgaaaagaaa ggcctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      181 atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
      241 aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa ttatcctgat
      301 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      361 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      421 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      481 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      541 aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca acgcccatta
      601 aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat ggattttaga
      661 aaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa
      721 tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga agcattttat
      781 tctagcttaa tttgacaaat cataacctat aaaaaaacaa aataaaaaag cagaaataac
      841 tggct
//
LOCUS       DAZKOM010000248          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000248 DAZKOM010000000
VERSION     DAZKOM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.257"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8NF_003005"
     CDS             <1..672
                     /locus_tag="ACG8NF_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0473385.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKOM010000249          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000249 DAZKOM010000000
VERSION     DAZKOM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.258"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACG8NF_003006"
     CDS             complement(<1..209)
                     /locus_tag="ACG8NF_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473386.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNE"
     gene            complement(216..764)
                     /locus_tag="ACG8NF_003007"
     CDS             complement(216..764)
                     /locus_tag="ACG8NF_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0473387.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          272 a          172 c           82 g          285 t
ORIGIN      
        1 ttttcattga ctataactag ttcatctggt aaaaaaatta accaacaaac atcgacaaac
       61 agtaaatgat ataatccatt tccacctcca agcatattca atactggtga acatgcctta
      121 gaaacacgaa tatagttatg gtttaaatag atattttctt taaacattgc ttgtttaaaa
      181 gccatatgct cttgttctaa acttttcata aacttttact ctaatgcaca gctagcagca
      241 gctgctgcaa ttcctgctac aatatttacc ttagctaact ctttagctgc agcttttcct
      301 aattgttttt ctgcaatttt taataataaa tccgcagctt tttcaaaatt aaaagctttt
      361 aatgcttcaa taaaatctcc ctgcgtacct atagcccaag ccactcctcc tccaggaaga
      421 gaattaatgg ctacacatcc agcatatcgt ttccaatcaa ctttctccat tctaggaaca
      481 ataatatgtt cataaaagac tcttgctgct cttccttgtt cgttatcttg actgattcgt
      541 tcacgaatag caccaaaatc ctttatatgg tatacaccat gttcgtctaa aaaaccaact
      601 gtatcaaaat aaaaagttaa ttcatctact aatttattct ttatttctaa atcaactgta
      661 ttcacttttt ctgtggcata cacacttgtt ccagtactaa caaaagtact taaacctaat
      721 aagaaaacac ctaaaacacc aaatatttta attattcttt tcataatttc tcctccataa
      781 aaaataagtt actttttaaa tatcttgttt a
//
LOCUS       DAZKOM010000250          809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000250 DAZKOM010000000
VERSION     DAZKOM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.259"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..574)
                     /locus_tag="ACG8NF_003008"
     CDS             complement(<1..574)
                     /locus_tag="ACG8NF_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFQ0473388.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSII"
     gene            complement(567..>809)
                     /locus_tag="ACG8NF_003009"
     CDS             complement(567..>809)
                     /locus_tag="ACG8NF_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFQ0473389.1"
                     /translation="IGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQ
                     IKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          308 a          132 c           88 g          281 t
ORIGIN      
        1 aaataataga agtatatcca aacatagctc caacatttgg acttgtatag acaccaaata
       61 accgatgttc aataaaacct tgtcttgcaa gtaatcctgg cgttcctgtt gggacatgat
      121 aagaaatcaa aaaaataaat tgatagatag atattagcgc agctaaaaaa attatgataa
      181 tgaaaacatt attaaaatac accatttttt tctttttttc ctcttcactt tgtgaaaagt
      241 ctgcaggaaa aattactatc aaagtaatca gcaaatatcc aaaattataa atagatggaa
      301 ttaaaaaatt ttctctgtta atgattatag ttaagataaa actaatcatt gctccaatca
      361 gtagccaaga aaaacgcatt ttaaaaaaat ttttctttat aaaaacctca tacaaaacta
      421 taattccacc ccaagcaaaa gctattttca tcgggatatt agttacatta aaagtactag
      481 gcataaaata aagcagacaa atgattaaat ttattactcg aaacgcatct cgacttaata
      541 aactcttctc taaaacaggt actattctat tcatctatat ttccaacttt caaaatttct
      601 ttatcaattc tatttaatag tttatcagag taatccgatt taacttttct tacaaataat
      661 ttttcacttt cttttatttg atctatatcc ctttcatcaa accatttgtt catggaagga
      721 tcaataatat gaatatttgc taatttcgaa gtttgtaaag tagtttcttc aatatttttc
      781 aattcaagtg gtaaatctgg tcctccaat
//
LOCUS       DAZKOM010000251          794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000251 DAZKOM010000000
VERSION     DAZKOM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.260"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8NF_003010"
     CDS             85..624
                     /locus_tag="ACG8NF_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473390.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          309 a           97 c          146 g          242 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aatt
//
LOCUS       DAZKOM010000252          792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000252 DAZKOM010000000
VERSION     DAZKOM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.261"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>792
                     /locus_tag="ACG8NF_003011"
     CDS             <1..>792
                     /locus_tag="ACG8NF_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0473391.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT          269 a          137 c          153 g          233 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata at
//
LOCUS       DAZKOM010000253          765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000253 DAZKOM010000000
VERSION     DAZKOM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.262"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8NF_003012"
     CDS             complement(<1..339)
                     /locus_tag="ACG8NF_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473392.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="ACG8NF_003013"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="ACG8NF_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0473393.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          229 a          124 c          124 g          288 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttat
//
LOCUS       DAZKOM010000254          759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000254 DAZKOM010000000
VERSION     DAZKOM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.263"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG8NF_003014"
     CDS             complement(<1..613)
                     /locus_tag="ACG8NF_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0473394.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          230 a          146 c          144 g          239 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatca
//
LOCUS       DAZKOM010000255          733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000255 DAZKOM010000000
VERSION     DAZKOM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.264"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            308..706
                     /gene="spxA"
                     /locus_tag="ACG8NF_003015"
     CDS             308..706
                     /gene="spxA"
                     /locus_tag="ACG8NF_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0473395.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          264 a          104 c          134 g          231 t
ORIGIN      
        1 ataaaaaatc ttaagaaatc ggtttcattt agcttttgag ataaaataat agtacagttt
       61 tatacgctgt ttcatatgtt tagaaaattt gaattagatt tgaaaactct aatttttaaa
      121 tgataaaaaa agcgagaatg ctggttttac tgtatattaa ccttagataa ctttcatttt
      181 tttaaaaaaa ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat aatagaaaca
      241 gatgggagat tattatgatc ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg
      301 gtgcacgttg ttaacattat atacttcccc aagttgtact tcctgcagaa aggctcgtgc
      361 gtggttgcaa gaacatcaga ttccatttgt tgaacgtaat attttttcag aaccattgaa
      421 cagttctgaa ttaaaagcga ttttacaaat gacagaagac ggaacagaag aaatcatctc
      481 gacacgttcc aaagtattcc aaaaactgaa catggattta gatgatctgc cgttacaaga
      541 attattggaa cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca tgattgatga
      601 taaacgtctg caagttggtt ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt
      661 acgacaatta gaattacgtc aagcacaatt gatggctgga ttatagaagg ataatacgaa
      721 acttggaaaa gac
//
LOCUS       DAZKOM010000256          730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000256 DAZKOM010000000
VERSION     DAZKOM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.265"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>730
                     /locus_tag="ACG8NF_003016"
     CDS             <1..>730
                     /locus_tag="ACG8NF_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0473396.1"
                     /translation="FISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYL
                     RIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYI
                     SNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSID
                     LAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNI
                     LLNMIFIPRLGIIAAGYTTVFTYFL"
BASE COUNT          226 a           93 c          135 g          276 t
ORIGIN      
        1 ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt
       61 tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt
      121 agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag
      181 atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc
      241 acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc
      301 ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg
      361 ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag
      421 taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga
      481 tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat
      541 aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg aattttatga
      601 gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt
      661 aaacatgatt ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac
      721 gtatttttta
//
LOCUS       DAZKOM010000257          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000257 DAZKOM010000000
VERSION     DAZKOM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.266"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8NF_003017"
     CDS             <1..>725
                     /locus_tag="ACG8NF_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0473397.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKOM010000258          713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000258 DAZKOM010000000
VERSION     DAZKOM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.267"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..239
                     /locus_tag="ACG8NF_003018"
     CDS             <1..239
                     /locus_tag="ACG8NF_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFQ0473398.1"
                     /translation="GNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGK
                     ETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            373..612
                     /locus_tag="ACG8NF_003019"
     CDS             373..612
                     /locus_tag="ACG8NF_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0473399.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          302 a           83 c          135 g          193 t
ORIGIN      
        1 gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat cctgaatgta
       61 agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat gtaaaagaat
      121 tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca
      181 atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc tcaaaataaa
      241 cataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta tcacaaatca
      301 cttatcacaa atcacaagtg atttgtgatt gttgatgata aaataagaat aagaagaaat
      361 agaaagaagt gagtgattgt gggaaattta ggcgcacaaa aagaaaaacg aaatgataca
      421 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
      481 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
      541 ataagactta gaagcaaact taagagtgtg ttgatagtgc attatcttaa aattttgtat
      601 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc
      661 atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa aca
//
LOCUS       DAZKOM010000259          712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000259 DAZKOM010000000
VERSION     DAZKOM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.268"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..479)
                     /locus_tag="ACG8NF_003020"
     CDS             complement(<1..479)
                     /locus_tag="ACG8NF_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0473400.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     "
BASE COUNT          219 a          133 c          109 g          251 t
ORIGIN      
        1 gcaagcaatc ctagctcttc tttaatattc tcctggatca tttgagccag cttctttctc
       61 cgttgacttc ttgttccttc agtcgtaaaa agattcattg atctggtcac ttttaagatc
      121 gattcatcga tcgagtaaat caataaatct tcgtctgcta cataccttct gaaaatattg
      181 tttacctgca tatttcgctt aatatagaga ttcatgcgtg gtggaacgac atgtaacgtt
      241 ttaggaaaag gttgcggtag atctcgtggt cgactgacat tagaaatacc gtacttcttc
      301 tttgcagctg gtgaagaagc taaaatcaac cctgatcctg tattatccgc tcgactcatg
      361 acgactaatt cagtcgttaa tggatctaaa ttcctttcta tacattcgac acttgcgtaa
      421 aagcttttca tatcgatcaa aaagtaatca tttactggct cttttgaata atccatcata
      481 aataacactc cattcttcca cattatacaa acatacgttc gtttttgtaa agaagaatcg
      541 attaaatgta caaaaaaaga agcataggta agttatttcc ctacttcttc tgttgatttg
      601 taaaatcaac acattgtatc actttattaa atggatattt actattagtc ttgtgaaaat
      661 ttgaaaataa gtgtcgtatt atccagtttt gcttagttat attagaaaaa aa
//
LOCUS       DAZKOM010000260          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000260 DAZKOM010000000
VERSION     DAZKOM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.269"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACG8NF_003021"
     CDS             <1..600
                     /locus_tag="ACG8NF_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0473401.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAZKOM010000261          690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000261 DAZKOM010000000
VERSION     DAZKOM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.270"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8NF_003022"
     CDS             complement(<1..461)
                     /locus_tag="ACG8NF_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0473402.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          219 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAZKOM010000262          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000262 DAZKOM010000000
VERSION     DAZKOM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.271"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..347)
                     /locus_tag="ACG8NF_003023"
     CDS             complement(<1..347)
                     /locus_tag="ACG8NF_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0473403.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAIL"
BASE COUNT          232 a          115 c          120 g          203 t
ORIGIN      
        1 atgagaatcg cctgcttcag ttcctttgtt agttgatgat gttcatcaac taagtccgtt
       61 ttcgcagaaa aggtccgtcc acaatcatgg cagagaaaac gggtacgttt taattctaaa
      121 atagtcaact tttctttgac tttcaacatc tgagtgcgtg tcgtatagga accgttgcgg
      181 ataatacgaa tcgatccaca gtgttcacaa tgggaaggtg agtcatccaa tctcccttta
      241 ataaactgcg cttgaacgcc tttatattct ttttcaatca gccattcttc atcaaaaaat
      301 aaatgtttat ctgttaatcc gagtaatttt ctagtatgat tatccattga gagactcctt
      361 tggtagggtg acgttttggt cgactaaatt ttaccaaaag tgagtctctt tttgagttga
      421 aacgaagaaa aaagtgttag tagaaaatcg ctttcgattt tccaccaaca caaaatatta
      481 tagaaccgaa ttccttaggc agcttttgct gtgtagaaag gaataaataa taacgaaagt
      541 tcaaaagcta atgccgacta ttctagacat taaagaagac gaaaacgaac gataaagaaa
      601 gaaaagttat gaataatcgt ttattgaaaa aaacttgaaa aaatacaaaa aattcgttga
      661 ccaagctaaa
//
LOCUS       DAZKOM010000263          605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000263 DAZKOM010000000
VERSION     DAZKOM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.273"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..233
                     /locus_tag="ACG8NF_003024"
     CDS             3..233
                     /locus_tag="ACG8NF_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473404.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            248..>605
                     /locus_tag="ACG8NF_003025"
     CDS             248..>605
                     /locus_tag="ACG8NF_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473405.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMAS"
BASE COUNT          235 a          100 c          121 g          149 t
ORIGIN      
        1 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
       61 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
      121 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
      181 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
      241 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
      301 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
      361 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
      421 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
      481 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
      541 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
      601 tagcg
//
LOCUS       DAZKOM010000264          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000264 DAZKOM010000000
VERSION     DAZKOM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.274"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG8NF_003026"
     CDS             <1..580
                     /locus_tag="ACG8NF_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0473406.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAZKOM010000265          583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000265 DAZKOM010000000
VERSION     DAZKOM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.275"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACG8NF_003027"
     CDS             complement(38..>583)
                     /locus_tag="ACG8NF_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0473407.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAZKOM010000266          549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000266 DAZKOM010000000
VERSION     DAZKOM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.276"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACG8NF_003028"
     CDS             143..400
                     /locus_tag="ACG8NF_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473408.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          236 a           69 c           76 g          168 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa attagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgacaaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacat
//
LOCUS       DAZKOM010000267          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000267 DAZKOM010000000
VERSION     DAZKOM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.277"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8NF_003029"
     CDS             complement(<1..132)
                     /locus_tag="ACG8NF_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0473409.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKOM010000268          509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000268 DAZKOM010000000
VERSION     DAZKOM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.278"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..440)
                     /gene="dfr"
                     /locus_tag="ACG8NF_003030"
     CDS             complement(<1..440)
                     /gene="dfr"
                     /locus_tag="ACG8NF_003030"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DfrD/DfrG/DfrK family trimethoprim-resistant
                     dihydrofolate reductase"
                     /protein_id="HFQ0473410.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKN"
BASE COUNT          153 a          114 c           50 g          192 t
ORIGIN      
        1 tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga
       61 aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa
      121 gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta
      181 cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc
      241 ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg
      301 ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc
      361 ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca
      421 gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc
      481 ttatttctat caaaaaacct tttctcatt
//
LOCUS       DAZKOM010000269          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000269 DAZKOM010000000
VERSION     DAZKOM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.279"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..327
                     /locus_tag="ACG8NF_003031"
     CDS             <1..327
                     /locus_tag="ACG8NF_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473411.1"
                     /translation="KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQ
                     REGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRK
                     LSEVNN"
BASE COUNT          199 a           66 c           90 g          140 t
ORIGIN      
        1 aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga agataatcca
       61 tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg agatcttatt
      121 cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt taaaggtcga
      181 ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct atataaagaa
      241 ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc ttcattatac
      301 aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg caatattttt
      361 aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga aaagcccttt
      421 gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata
      481 aattcggccc tataa
//
LOCUS       DAZKOM010000270          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000270 DAZKOM010000000
VERSION     DAZKOM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.280"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..>483
                     /locus_tag="ACG8NF_003032"
     CDS             102..>483
                     /locus_tag="ACG8NF_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFQ0473412.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTL"
BASE COUNT          192 a           57 c           66 g          168 t
ORIGIN      
        1 ataaaacttt gataaaatac aaatgttttt tatttttatg tgtgattagt tagaacttac
       61 ttaaatccta ttataatatt tttgttaatg attttaataa aatgattgga gagaaaatga
      121 tggatcaaaa agcgtttatc gaatctattg attctacaat cgatagatta aaaaaagaaa
      181 ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc ttatcagcta
      241 gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg attgtttcaa
      301 ttgcttcagc aataattaca attatacaaa gtgggatgtc tgcatttaat tatcaagata
      361 aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg ttatatataa
      421 cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc gttacaaccc
      481 ttc
//
LOCUS       DAZKOM010000271          476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000271 DAZKOM010000000
VERSION     DAZKOM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.281"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>476
                     /locus_tag="ACG8NF_003033"
     CDS             22..>476
                     /locus_tag="ACG8NF_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0473413.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DAZKOM010000272          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000272 DAZKOM010000000
VERSION     DAZKOM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.282"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          153 a           91 c           56 g          171 t
ORIGIN      
        1 acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag atgaaagatc
       61 aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact actttttcct
      121 ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct ttcaatgctt
      181 gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc tagaacatat
      241 tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa tttcgatagg
      301 ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc aagataggaa
      361 tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt cacgctccac
      421 aaaattgttt tatacactac tattcagccc attcaacgac caatgaattt t
//
LOCUS       DAZKOM010000273          440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000273 DAZKOM010000000
VERSION     DAZKOM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.283"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..205)
                     /locus_tag="ACG8NF_003034"
     CDS             complement(74..205)
                     /locus_tag="ACG8NF_003034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473414.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            complement(178..300)
                     /locus_tag="ACG8NF_003035"
     CDS             complement(178..300)
                     /locus_tag="ACG8NF_003035"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473415.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          145 a           82 c           62 g          151 t
ORIGIN      
        1 tggcaaaaat ttcaatcttt aaatggcgaa aaacaaagag cgtgagaccc tatccttatc
       61 tcacgctctt tgtctatttt tctaacaaat caagtatttc ttgaggagaa tcaataatta
      121 tatctacact ttgaaaattt tctatatttt tggctcccca tttagctaaa ccaaatgcta
      181 tcccacaagc tttcgcactt tgcatgtcat ataatgaatc accaatataa atcgtttctt
      241 attttttatg cccaattcat caattgcctt ttgtatgggg tccggaaaag gtttatgcaa
      301 cttagtatct gaagctgtaa tttgtatatc aaaatataga tttaacccgt ggtgctagtt
      361 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcatata
      421 gtagtctcaa aactcccaag
//
LOCUS       DAZKOM010000274          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000274 DAZKOM010000000
VERSION     DAZKOM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.284"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8NF_003036"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8NF_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKOM010000275          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000275 DAZKOM010000000
VERSION     DAZKOM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.285"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>418
                     /locus_tag="ACG8NF_003037"
     CDS             <1..>418
                     /locus_tag="ACG8NF_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0473416.1"
                     /translation="YRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTK
                     GELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPI
                     TYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          140 a           77 c           71 g          130 t
ORIGIN      
        1 tatagagcgg tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc
       61 actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag
      121 aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa
      181 ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca
      241 acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt
      301 ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta
      361 tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKOM010000276          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000276 DAZKOM010000000
VERSION     DAZKOM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.286"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8NF_003038"
     rRNA            <1..>411
                     /locus_tag="ACG8NF_003038"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKOM010000277          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000277 DAZKOM010000000
VERSION     DAZKOM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.287"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>404
                     /locus_tag="ACG8NF_003039"
     CDS             <1..>404
                     /locus_tag="ACG8NF_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0473417.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAZKOM010000278          376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000278 DAZKOM010000000
VERSION     DAZKOM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.288"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           52 c           54 g          136 t
ORIGIN      
        1 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttatatt
       61 gtgagataat aataaagaag agtttaaagc gttccccaaa aaccactgcc ccataagtgt
      121 gttacgcttt aaactctttt atatttgaag ccattaaaaa gcataccata tttttgaaaa
      181 aaagtgagaa aaaaggctta caattggagt ggtagttaat tagtgactta tttttgattt
      241 tatagcactg atactataaa atatagatat catcatatta cacaatctta atactaactt
      301 aaaaaatatc tcctttcaca agtatggtga taaaattcgt tccgggctac ctttttaggt
      361 agcctacttt aatctt
//
LOCUS       DAZKOM010000279          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000279 DAZKOM010000000
VERSION     DAZKOM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.289"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..>368
                     /locus_tag="ACG8NF_003040"
     CDS             161..>368
                     /locus_tag="ACG8NF_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473418.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
BASE COUNT          120 a           33 c           63 g          152 t
ORIGIN      
        1 tactttttct agttttaatt ttcagtttct attaaaaaat aatttttatg gtttattgaa
       61 ataaggttgc gaaggaagat gtagagttat ttgcactgtt gtgttggtat atctcttaat
      121 gctgtgtctt tttggggaga ttaattagtg gagtgattat atgagtatag atgattgttt
      181 agaagaacaa ataagattat ttttaaagtt tttaaaagtt aaagttcaaa ataactactt
      241 cacatgttat agttatgcac accctgtttt aaatagtctt ccttttaatt ctttagtgca
      301 aggattgatt acaaatattg ttttattagt ctgtacacca aatgaaatat ttgtaaaaaa
      361 gataggca
//
LOCUS       DAZKOM010000280          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000280 DAZKOM010000000
VERSION     DAZKOM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.290"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8NF_003041"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8NF_003041"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKOM010000281          353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000281 DAZKOM010000000
VERSION     DAZKOM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.291"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>353
                     /locus_tag="ACG8NF_003042"
     CDS             <1..>353
                     /locus_tag="ACG8NF_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473419.1"
                     /translation="YIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKRIESG
                     QIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVAN
                     NIGIQIRDMTESEVY"
BASE COUNT          145 a           57 c           72 g           79 t
ORIGIN      
        1 tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt caaaggattt
       61 tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga taaaaaacga
      121 atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact acacgattga aaaagtgttt
      181 gatatcacac aaacacagtt aaaacctgaa gactatccga aaatctttcc gaaccgtgtc
      241 tttgattttc aattggataa aaaaggacag tcagaacttc aagctgggat tcaagcggta
      301 gccaacaata ttggaataca aattcgagat atgacagaaa gtgaagtcta tca
//
LOCUS       DAZKOM010000282          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000282 DAZKOM010000000
VERSION     DAZKOM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.292"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8NF_003043"
     CDS             <1..>321
                     /locus_tag="ACG8NF_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0473420.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKOM010000283          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000283 DAZKOM010000000
VERSION     DAZKOM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.293"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..>314)
                     /locus_tag="ACG8NF_003044"
     CDS             complement(82..>314)
                     /locus_tag="ACG8NF_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0473421.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           99 a           46 c           57 g          112 t
ORIGIN      
        1 aaaaagatgt tggtgaagtt caccaacatc aaaaagtata gagccgatag aaggactgac
       61 cttctgtctc agattttttt gttatttcac ttgaatcaat tgattgatca aaaaaatcct
      121 gattttcatg ttctcaaatg atttaaatcc ataagataat cgtttcagtg tcttgatatg
      181 gggaatcttg gcctctattt tgccattaga atacgggtaa atcatagcat tgtaaatacc
      241 ttcttcatac gttaaaaggt tttgaagttt cgtgcgaagt tgtgcatcaa gtgtttcagg
      301 aagatttttt aata
//
LOCUS       DAZKOM010000284          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000284 DAZKOM010000000
VERSION     DAZKOM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.294"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..301
                     /locus_tag="ACG8NF_003045"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACG8NF_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAZKOM010000285          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000285 DAZKOM010000000
VERSION     DAZKOM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.295"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACG8NF_003046"
     CDS             <1..>299
                     /locus_tag="ACG8NF_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0473422.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZKOM010000286          269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000286 DAZKOM010000000
VERSION     DAZKOM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.296"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG8NF_003047"
     CDS             <1..260
                     /locus_tag="ACG8NF_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473423.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
BASE COUNT           97 a           27 c           48 g           97 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaac
//
LOCUS       DAZKOM010000287          237 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000287 DAZKOM010000000
VERSION     DAZKOM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.297"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>237
                     /locus_tag="ACG8NF_003048"
     CDS             <1..>237
                     /locus_tag="ACG8NF_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFQ0473424.1"
                     /translation="HDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHG
                     SQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAP"
BASE COUNT           89 a           38 c           36 g           74 t
ORIGIN      
        1 catgattatc ctagaattaa taggaataac acaactgcat ttaaaatttt tagagctata
       61 attaatcgtt gtctgaggat gattaaactt cccccaaaaa ttagacacca taaatttgat
      121 ttgtatcatg gttctcaatg gtgggctttg tcgaaagaat gcctaacaga attaatacaa
      181 atgtatgaac aaaatcaaca agactacctg aattttaaaa taggtatgtt tgcccca
//
LOCUS       DAZKOM010000288          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000288 DAZKOM010000000
VERSION     DAZKOM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.298"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>232)
                     /locus_tag="ACG8NF_003049"
     CDS             complement(<1..>232)
                     /locus_tag="ACG8NF_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473425.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DAZKOM010000289          231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000289 DAZKOM010000000
VERSION     DAZKOM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.299"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           89 a           20 c           44 g           78 t
ORIGIN      
        1 aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc
       61 tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg
      121 agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat
      181 aaattaaata ggtgcataaa agcattacac ttaaaataca ttgttgctta c
//
LOCUS       DAZKOM010000290          228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000290 DAZKOM010000000
VERSION     DAZKOM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.300"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..>228
                     /locus_tag="ACG8NF_003050"
     CDS             19..>228
                     /locus_tag="ACG8NF_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0473426.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
BASE COUNT           77 a           30 c           37 g           84 t
ORIGIN      
        1 tagatcggag taatggctat gctttttaat gagacacaat tatggtttaa atttgatcct
       61 tcgaatagat ttatcaaaga tttttataag gtgtgggatt cagaagtttt ctttctagca
      121 atcgaagata gcttattaat caatctctac tattctaata agaactactt taaaatccct
      181 gctgcaaaaa ctagaatgaa gaaagatgta tactttttgt ttgatatc
//
LOCUS       DAZKOM010000291          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000291 DAZKOM010000000
VERSION     DAZKOM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.301"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG8NF_003051"
     CDS             <1..>224
                     /locus_tag="ACG8NF_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0473427.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKOM010000292          220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000292 DAZKOM010000000
VERSION     DAZKOM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.302"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG8NF_003052"
     rRNA            complement(<1..175)
                     /locus_tag="ACG8NF_003052"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAZKOM010000293          213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000293 DAZKOM010000000
VERSION     DAZKOM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.303"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>213)
                     /locus_tag="ACG8NF_003053"
     CDS             complement(37..>213)
                     /locus_tag="ACG8NF_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0473428.1"
                     /translation="NLYYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYD
                     YLKYTFVDPERYKD"
BASE COUNT           60 a           44 c           35 g           74 t
ORIGIN      
        1 aatccgttcc gggctacctt ttttaggtag cctactttaa tctttatacc tttctggatc
       61 aacgaaagta tacttcaaat agtcataacg ccgatgatcg ctccgtgcgt ctggcacatc
      121 agtcacgata tcaaacaaaa aatacacatc tttcttcatt ctggtttttg ttgccggaat
      181 tttaaagtag ttcttattag agtaatagag att
//
LOCUS       DAZKOM010000294          207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000294 DAZKOM010000000
VERSION     DAZKOM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.304"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           77 a           29 c           26 g           75 t
ORIGIN      
        1 ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta tacgaactaa
       61 tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat atattaaaaa
      121 gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa aatctgcgaa
      181 aataaaaaat gctcaaaccc ttataaa
//
LOCUS       DAZKOM010000295          206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000295 DAZKOM010000000
VERSION     DAZKOM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.305"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>206)
                     /locus_tag="ACG8NF_003054"
     CDS             complement(<1..>206)
                     /locus_tag="ACG8NF_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0473429.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DAZKOM010000296          205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000296 DAZKOM010000000
VERSION     DAZKOM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.denovo.306"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>205
                     /locus_tag="ACG8NF_003055"
     CDS             <1..>205
                     /locus_tag="ACG8NF_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0473430.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DAZKOM010000297        10792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.098, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000297 DAZKOM010000000
VERSION     DAZKOM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.098"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1170
                     /locus_tag="ACG8NF_003056"
     CDS             259..1170
                     /locus_tag="ACG8NF_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0473431.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1323..1808
                     /gene="vanZ-A"
                     /locus_tag="ACG8NF_003057"
     CDS             1323..1808
                     /gene="vanZ-A"
                     /locus_tag="ACG8NF_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0473432.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2327..2611
                     /locus_tag="ACG8NF_003058"
     CDS             2327..2611
                     /locus_tag="ACG8NF_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0473433.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2977..3276
                     /locus_tag="ACG8NF_003059"
     CDS             2977..3276
                     /locus_tag="ACG8NF_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473434.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3319..4809
                     /locus_tag="ACG8NF_003060"
     CDS             3319..4809
                     /locus_tag="ACG8NF_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0473435.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5157..5327
                     /locus_tag="ACG8NF_003061"
     CDS             5157..5327
                     /locus_tag="ACG8NF_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473436.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5341..5958
                     /locus_tag="ACG8NF_003062"
     CDS             5341..5958
                     /locus_tag="ACG8NF_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0473437.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5958..6896
                     /locus_tag="ACG8NF_003063"
                     /pseudo
     CDS             5958..6896
                     /locus_tag="ACG8NF_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(7589..8236)
                     /locus_tag="ACG8NF_003064"
     CDS             complement(7589..8236)
                     /locus_tag="ACG8NF_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0473438.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(8366..9304)
                     /locus_tag="ACG8NF_003065"
     CDS             complement(8366..9304)
                     /locus_tag="ACG8NF_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0473439.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            9795..10792
                     /locus_tag="ACG8NF_003066"
                     /pseudo
     CDS             9795..10792
                     /locus_tag="ACG8NF_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         3909 a         1670 c         1878 g         3335 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat
     1201 gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac
     1261 gttaactagg acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta
     1321 tattgggaaa aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt
     1381 tagtgttatt caaattacaa tacaatattt tatcagtatt taattatcat caaagaagtc
     1441 ttaacttgac tccatttact gctactggga atttcagaga gatgatagat aatgttataa
     1501 tctttattcc atttggcttg cttttgaatg tcaattttaa agaaatcgga tttttaccta
     1561 agtttgcttt tgtactggtt ttaagtctta cttttgaaat aattcaattt atcttcgcta
     1621 ttggagcgac agacataaca gatgtaatta caaatactgt tggaggcttt cttggactga
     1681 aattatatgg tttaagcaat aagcatatga atcaaaaaaa attagacaga gttattattt
     1741 ttgtaggtat acttttgctc gtattattgc tcgtttaccg tacccattta agaataaatt
     1801 acgtgtaaga tgtctaaatc aagcaatctg atctttcata cacataaaga tattgaatga
     1861 attggattag atggaaaacg ggatgtgggg aaactcgccc gtaggtgtga agtgagggga
     1921 aaaccggtga taaagtaaaa agcttaccta acactatagt aacaaagaaa gcccaattat
     1981 caattttagt gctgaggaat tggtctcttt aataaatttc cttaacgttg taaatccgca
     2041 ttttcctgac ggtaccccca ctagcagcca tgcttttttt gaatctttgt aggacgtttt
     2101 aaagagaatt acccacttta aaattaaaca tgcgcttaga atcgctttag gaaacacgat
     2161 ccagtccaat aatcgtcgat aaaaactttt gaaaaaggtt ggtgaaatta cctacttttg
     2221 gaataatcac aaatcacaag tgattaatca cttgtttatt aagatattaa aagctataat
     2281 ttaaataaag cgtgaatttt attacacaaa aagagggggg agaaacttgg aactagcatt
     2341 tagagaaagc ttaaaaaaga tgagaggtac caaatcaaaa gaaaaattct cccaagaatt
     2401 agaaatgagt agatcaaatt attcacgaat agaatcagga aaatcagatc caaccataaa
     2461 aacactagaa caaattgcaa agttaactaa ctcaacgcta gtagtggatt taatcccaaa
     2521 tgagccaaca gaaccagaac cagaaacaga atcagaacaa gtaacattgg atttagaaat
     2581 ggaagaagaa aaaagcaatg acttcgtgtg aataatgcac gaaatcgttg cttatttttt
     2641 ttaaaagcgg tatactagat ataacgaaac aacgaactga atagaaacga aaaaagagcc
     2701 atgacacatt tataaaatgt ttgacgacat tttataaatg catagcccga taagattgcc
     2761 aaaccaacgc ttatcagtta gtcagatgaa ctcttccctc gtaagaagtt atttaattaa
     2821 ctttgtttga agacggtata taaccgtact atcattatat agggaaatca gagagttttc
     2881 aagtatctaa gctactgaat ttaagaattg ttaagcaatc aatcggaaat cgtttgattg
     2941 ctttttttgt attcatttat agaaggtgga gtttgtatga atcatgatga atgtaaaact
     3001 tatataaaaa atagtttatt ggagataaga aaattagcaa atatctatac actagaaacg
     3061 tttaagaaag agttagaaaa gagaaatatc tacttagaaa caaaatcaga taagtatttt
     3121 tcttcggagg gggaagatta tatatataag ttaatagaaa ataacaaaat aatttattcg
     3181 attagtggaa aaaaattgac ttataaagga aaaaaatctt tttcaaaaca tgcaatattg
     3241 aaacagttga atgaaaaagc aaaccaagtt aattaaacaa cctattttat aggatttata
     3301 ggaaaggaga acagctgaat gaatatccct tttgttgtag aaactgtgct tcatgacggc
     3361 ttgttaaagt acaaatttaa aaatagtaaa attcgctcaa tcactaccaa gccaggtaaa
     3421 agcaaagggg ctatttttgc gtatcgctca aaatcaagca tgattggcgg tcgtggtgtt
     3481 gttctgactt ccgaggaagc gattcaagaa aatcaagata catttacaca ttggacaccc
     3541 aacgtttatc gttatggaac gtatgcagac gaaaaccgtt catacacgaa aggacattct
     3601 gaaaacaatt taagacaaat caataccttc tttattgatt ttgatattca cacggcaaaa
     3661 gaaactattt cagcaagcga tattttaaca accgctattg atttaggttt tatgcctact
     3721 atgattatca aatctgataa aggttatcaa gcatattttg ttttagaaac gccagtctat
     3781 gtgacttcaa aatcagaatt taaatctgtc aaagcagcca aaataatttc gcaaaatatc
     3841 cgagaatatt ttggaaagtc tttgccagtt gatctaacgt gtaatcattt tggtattgct
     3901 cgcataccaa gaacggacaa tgtagaattt tttgatccta attaccgtta ttctttcaaa
     3961 gaatggcaag attggtcttt caaacaaaca gataataagg gctttactcg ttcaagtcta
     4021 acggttttaa gcggtacaga aggcaaaaaa caagtagatg aaccctggtt taatctctta
     4081 ttgcacgaaa cgaaattttc aggagaaaag ggtttaatag ggcgtaataa cgtcatgttt
     4141 accctctctt tagcctactt tagttcaggc tattcaatcg aaacgtgcga atataatatg
     4201 tttgagttta ataatcgatt agatcaaccc ttagaagaaa aagaagtaat caaaattgtt
     4261 agaagtgcct attcagaaaa ctatcaaggg gctaataggg aatacattac cattctttgc
     4321 aaagcttggg tatcaagtga tttaaccagt aaagatttat ttgtccgtca agggtggttt
     4381 aaattcaaga aaaaaagaag cgaacgtcaa cgtgttcatt tgtcagaatg gaaagaagat
     4441 ttaatggctt atattagcga aaaaagcgat gtatacaagc cttatttagt gacgaccaaa
     4501 aaagagatta gagaagtgct aggcattcct gaacggacat tagataaatt gctgaaggta
     4561 ctgaaggcga atcaggaaat tttctttaag attaaaccag gaagaaatgg tggcattcaa
     4621 cttgctagtg ttaaatcatt gttgctatcg atcattaaag taaaaaaaga agaaaaagaa
     4681 agctatataa aggcgctgac aaattctttt gacttagagc atacattcat tcaagagact
     4741 ttaaacaagc tagcagaacg ccctaaaacg gacacacaac tcgatttgtt tagctatgat
     4801 acaggctgaa aataaaaccc gcactatgcc attacattta tatctatgat acgtgtttgt
     4861 tttttctttg ctgtttagcg aatgattagc agaaatatac agagtaagat tttaattaat
     4921 tattaggggg agaaggagag agtagcccga aaacttttag ttggcttgga ctgaacgaag
     4981 tgagggaaag gctactaaaa cgtcgagggg cagtgagagc gaagcgaaca cttgattttt
     5041 taattttcta tcttttatag gtcattagag tatacctatt tgtcctaata tgattttagc
     5101 agtataattg acttggtgaa taggtcattt aagttgggca taataggagg agtaaaatga
     5161 aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt ggctattttt aatgagcaac
     5221 aatatgcgca agattttatc gcttacgaaa agacaatttc tgataagcaa tttgaaattg
     5281 aaaaagtaga tattgctgat tggttattgc aaccgagaga attttagagg ttggttgaaa
     5341 atggctaaaa ttggttatgc acgtgtcagt agcaaagaac agaacttaga tcggcaatta
     5401 caagcgttac agggcgtttc taaggtcttt tcagacaaat taagcggtca atcggtcgaa
     5461 cgcccacaat tacaagctat gcttaactat attcgtgaag gggatattgt tattgttact
     5521 gaattagatc gattaggacg aaataataaa gaattaacag aattgatgaa tcaaattcaa
     5581 attaaggggg caaccctgga agtcttaaat ttaccctcaa tgaatggtat tgaagatgaa
     5641 aatttaaggc gtttgattaa tagccttgtc attgaattgt acaagtatca agcagaatca
     5701 gaacgaaaaa aaattaagga acgtcaggca caaggaatcg aaattgctaa gaaaaaaggc
     5761 aaattcaaag gtcgtcagca taaatttaaa gaaaatgatc cacgtttaaa gcatgctttc
     5821 gatttgtttt tgaatggttt atccgataaa gaagttgaag aacaaactgg aatcaatcgc
     5881 cgaacgttta gaaggtatcg agcaagatat aacgtgacag tcgatcaaag aaaaaacaat
     5941 gaaaagaggg atagttaatg agtacggtta ttttagctga aaaaccaagc caggcattag
     6001 cctacgcaag tgctttaaaa caaagcacca aaaaagacgg ttattttgag atcaaagacc
     6061 cactatttac agatgaaacg tttatcacct ttggttttgg gcatttagtg gaattagcag
     6121 aaccaggtca ttatgacgaa aagtggcaaa attggaaact tgaatctttg ccgatttttc
     6181 ctgatcgata cgattttgaa gttgcaaaag ataagggaaa gcagtttaaa attgttgcag
     6241 aacttctcaa aaaggcaaat acaattattg ttgcaacaga tagcgacaga gaaggtgaaa
     6301 atatcgcctg gtcgattatc cataaagcaa atgccttttc aaaagataaa acatttaaaa
     6361 gactatggat caatagctta gaaaaagatg taatccgaag cggttttcaa aatttgcaac
     6421 ctggaatgaa ttactatccc ttttatcaag aagcgcaaac acgccaaatt gccgattggt
     6481 tgatcggcat gaacgcaagc cctttgtata cgttaaattt acaacagaag ggcgtacaag
     6541 gtacattttc actaggacgt gttcaaacgc ccaccttata ccttattttt cagcgccagg
     6601 aagccataga gaattttaaa aaagaacctt ttttcgaggt ggaagctagt ataaaagtaa
     6661 accaagggtc gtttaagggc gttctaagcc ccacacagcg ttttaaaacc caagaggagc
     6721 ttttagcttt tgtttcttct aaacaagcta aaataggcaa tcaagagggg ataattgctg
     6781 atgttcaaac caaagagaag aaaacgaata gtccgagttt gttttcttta agtagtttgc
     6841 aatcaaaagt caatcagctt tataaagcga cagcgagcca aactttaaaa gctatttctt
     6901 ttttaataac ttaaaaataa acttaatgta acagcaagca cagtcaaggt atacaccttt
     6961 gacaaaaaat agcacattct ctatcgaaaa tttttgctta ttttttaaat tattttggga
     7021 aattttccca atcccttttt ctaactcaaa aaatataatc actcaaaatt taaaagggcg
     7081 cacttataca tcattttaaa aaattgatgt aacgtgctaa gttcaaaaca aagggcgcac
     7141 ttatacacga ttttcaatct tgtatatttc taacgaaaag cgtgcgccaa aaaaccccct
     7201 tcgtcaattt tgacaggggg ctttttgatg taaaaatttc tatcgaaatt taaaaattcg
     7261 cttcactcat gttataaaga cttaaaataa aataactctt taaaatcttt tgctagttgt
     7321 tcttcaatat tttttattcg gtgcatcttc caagtaaagt ataacacact agacttattt
     7381 actacgtttc ataagtcatt aatgcgtgtg ctctgcgagg ctagtttttg tgcaagcaca
     7441 aaaaatggac tgaataaatc agtccataag ttcaaaacca aattcaaaat caaaaccaca
     7501 agcaaccaaa aaatgtggtt gttatacgtt cataaatttt atgatcactt acgtgtataa
     7561 aattaaattc actttcaaaa tctaaaaact aaatccaatc atctacccta tgaattatat
     7621 cttgaaattc attcataaat agtgaagcat ggtaaccatc acatacagaa tgatgaagtt
     7681 gcagagcaac tggtatataa attttattat tctcactata aaatttacct atcgtaataa
     7741 taggcaataa aaagctgcta ttgttaccaa tatttaaatt aaatgaacta aaatcaatcc
     7801 aaggaatcat tgaaatcggt atggtgtttt caggtatcgg ttttttagga aacatttctt
     7861 ctttatcttt atattcaagc aagtcatttt tataattatt ataaaaagaa atgaagtttt
     7921 tatcagattc agtccaaatg ttagtaaatt tttcagtttg cttattaaaa actgtataca
     7981 aaggatttaa cttatcccaa taacctaatt tattctcact attaattcct gttctaaaca
     8041 ctttattttt atttacaact tccataattg cataaattaa agagggataa atttcatatc
     8101 ctttcttttt tatcatatct ttaaacaaag taatatcaat ttctttagta atgctataag
     8161 tagtttgctg attaaaatag tgttcaaaat attcttttct atcccaattt tctaattcaa
     8221 taatattaaa agtcatatat aacttcctcc taaattttaa atttttatat ttaggaggaa
     8281 taatcctctg attttttcat acgttatgtc acctcgtaaa tattaattat actgaattag
     8341 caatttttat caaataaaac ttattttact tccaaaacct aaattcacgt tgccaaaaat
     8401 caatctgctt ttgcaattgt ttttcgttcg cttttaaagt cgatttcatt aattccgtta
     8461 aatcaattgg agatatttct ctaatcaatt ttttaaattt agtcttagta ttcttactta
     8521 gctttcccca catactttct tcatgcaaca aagtataaac catagcttgc tcattaattt
     8581 tttctaaagt agcccacgca ggtttcaaga tgtgtaaatc attaaaacaa tcattccagt
     8641 aatcaaccat atctcttttt aattcaactt ctacacgcca taaatgttca gacacaactt
     8701 caacatctgc gttatcttta cgttcttgtt ttttattata aattctaata aatctatcac
     8761 tatcacggac accaaaatat tttgtttctg gcttgccatt acgaccataa aaaacagttt
     8821 tcttaactgc tttatcagtc attgcatagt aatcgctcaa atcatcttca aaatcaaaag
     8881 ctaagtctaa tcttgtaaaa ccgtcatctt ccatgtagtc gataatattt tgttttaacc
     8941 aaatcatttc ttcatgtgtg agtttattgg gattaaattc aacacgcata ttacgtctat
     9001 cccaagtatc tgcttttact ttgtcatatt cgatataaac tttttcttga agtgccttag
     9061 ctttaaactt tgtttgaagt atatcccaaa gtcgtatttg tggctctaca ctcataaagt
     9121 cagatagctt tttagcatta gttttgttca aatttccaac gattgtcatg gcatcaaaac
     9181 ttaatgcggg ttgagatttt cccaaagttt gaccacttaa ccggctatta cttaaccggc
     9241 tattagagac ggccggtttt tttagtgaga tttctgcagc taatttttca tcaattttct
     9301 tcatttttta tcgctcctaa aataattttt ggaacgaaaa aaggacgagt ggaatcgcaa
     9361 aataaatttc gcctgtatcc attcgtccat tttgttttgt attcaaaatc aaaaattgat
     9421 atactaatca tcgaatgaat aacaagtgtg gtttgttatt ccgggcgaga acgttggtag
     9481 cgttgctcgt ctttttttat gtcttttttt cgttaaaact attttaaaac taaatatatc
     9541 ttaaagcaaa taaattcaag gggctaaaag cgccccttaa aaaccccttg cctctgaaaa
     9601 gttttgtgtt tcctttccgt cggtttcaaa agctaattta ctcaaaaaaa tagatcaagg
     9661 gtaaatgcac tcactgcgtt cgcccttgag ctattttatt tcgcaaattt taacgcattt
     9721 gccgacgaaa agataaaaca cataaaaata tacgcagata tatttaaaac gtcttagaac
     9781 gcaaaatttt acgtttaaaa gctatgcaag gactgtatga agcaaaatta ttgagttatc
     9841 caagaacaga tacaccattt attacagaga acgaatttgc ttatttaaaa gcgaattttg
     9901 gcaaatatag cggtttttta ggacttgatc ttgaaatggt tcaaacagag cctagaaagc
     9961 gttatgtgga cggtagtaag gtacaggaac accacgccat tatcccaaca aaacaagtac
    10021 ctaccgaatc tgcattagcg aaaatggacg atttacaacg aaaaatttat gctttagtcg
    10081 ttaaaacgac cgttgccatg tttttacctg attacttgta tgaagaaacc aagatacaaa
    10141 cgaaagtagc tgatttgctg tttcaatcga tcggaaagac accaaagcaa gaagggtgga
    10201 aaattctttt caaacaacaa accaaagaag aaaaagagga cgttcaaacg ttaccactcg
    10261 ttatcattgg cgaacgtgcc gaagttggtg ttaagagtgt tgaaaaagaa acgcaaccgc
    10321 caaaagcttt tacagagggt acattattaa ctgctatgaa aacggcgaat aaaacggttg
    10381 atgatgaaga agcaatcaag attttacaag aagttgaggg gattggaaca gaagcgacaa
    10441 gagcaagtat tattgaagcg ttaaaacaaa aagaatatat ccaagtgatt aagaataagc
    10501 ttgttgtaac tgaaaaagga aaattattgt gccaggcagt tgaaagtcag caccttttaa
    10561 cgagtgctga aatgacggct aaatgggaaa cgtatttaaa aaaaatcggt aaaagagaag
    10621 gcaatcaaga gaactttatt acgaatatca aaaaattcat tgttcattta ctggaagctg
    10681 tacctaacga tatagaaaaa ctaaattttt ctgattacca ggaacagaaa gaaaaagaag
    10741 cagaaaaaag tattgtagga aaatgtccta agtgtggcaa caatattgta tt
//
LOCUS       DAZKOM010000298         9868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.104, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000298 DAZKOM010000000
VERSION     DAZKOM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.104"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1646
                     /locus_tag="ACG8NF_003067"
     CDS             144..1646
                     /locus_tag="ACG8NF_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0473440.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            1648..2385
                     /locus_tag="ACG8NF_003068"
     CDS             1648..2385
                     /locus_tag="ACG8NF_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0473441.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(2556..3749)
                     /locus_tag="ACG8NF_003069"
     CDS             complement(2556..3749)
                     /locus_tag="ACG8NF_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0473442.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(3828..4034)
                     /locus_tag="ACG8NF_003070"
     CDS             complement(3828..4034)
                     /locus_tag="ACG8NF_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0473443.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(4027..5169)
                     /locus_tag="ACG8NF_003071"
     CDS             complement(4027..5169)
                     /locus_tag="ACG8NF_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0473444.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            5681..6316
                     /locus_tag="ACG8NF_003072"
     CDS             5681..6316
                     /locus_tag="ACG8NF_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0473445.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            6470..6547
                     /locus_tag="ACG8NF_003073"
                     /pseudo
     CDS             6470..6547
                     /locus_tag="ACG8NF_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            6861..6908
                     /locus_tag="ACG8NF_003074"
                     /pseudo
     CDS             6861..6908
                     /locus_tag="ACG8NF_003074"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6924..8843
                     /gene="tet(M)"
                     /locus_tag="ACG8NF_003075"
     CDS             6924..8843
                     /gene="tet(M)"
                     /locus_tag="ACG8NF_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0473446.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            8962..9129
                     /locus_tag="ACG8NF_003076"
     CDS             8962..9129
                     /locus_tag="ACG8NF_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0473447.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(9189..9542)
                     /locus_tag="ACG8NF_003077"
     CDS             complement(9189..9542)
                     /locus_tag="ACG8NF_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473448.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         3035 a         1769 c         1940 g         3124 t
ORIGIN      
        1 ttttatctta ttatacatgt tatgttttat aacataaaat gttagaaact cgcatttttt
       61 tcattttttc gagcttacag tcttgtctga ttcagcactt ttcgcctata ataataaaat
      121 attggaaatt taggaggttg attatgaaaa gaaagcattt atttcactca tttgttttct
      181 taatgacagg acttgtgaca cttgcgtttg gtcatgttgc acaagctgac aacactacaa
      241 cggcagagtc aagcacaagc gagacaatgc aaaaaataga gcccaaaaaa gacgtatacg
      301 taattgccag tgactctgct tttgctccat ttgaatttca aaacgcaaag ggcgagtacg
      361 aaggcatcga tgtaaaattg atggaaaaca tcgccaaact gcaaggattt aacatccgaa
      421 tggactaccg tggtttctct gctgctttgc aagctttgga atcaggacaa gcagacggaa
      481 tgattgccgg aatgactgta acagatgaac gaaagaaaag ctatgatttt tctgattctt
      541 actttgatag cgggatccaa ttagctgtca aaaaaggaga tacttcgatc aagtcttatg
      601 aagatttgaa aggaaaaaca gtcggagcaa aagttggtac cgaaagtgct gaatttttaa
      661 gcgaccatga atctgaatat ggctattcta tcaaatattt tgatgctgct gatcaattgt
      721 acgacgggtt aaaagttggc gcaatcgatg caatgatgga tgattatccg gtaatcggtt
      781 atgcgatcaa acaaggacaa gaattggaaa caccgatcaa aaaagaatct ggcggaaaat
      841 atgcatttgc cgttaaaaaa ggacaaaatc ctgagcttct taatatgttc aatgaaggat
      901 tgaaagaatt aaaaagaaca ggcgaatacg ataagattat taaagaatat gtagcggatg
      961 gttcagaaac ttccagtgag tcttccactg ctgatgaatc aacatttctt ggacttatcc
     1021 aaaacaacta tcaagcattg ttgaaaggac tttggagaac aattctccta actttgatct
     1081 cctttgcttt ggctatagta atcggtgtga tttttggatt attcagtgta gcaccaatac
     1141 gcgctcttcg cacaattgct agtatttatg tcgatatcat ccgaggaatt ccaatgatgg
     1201 ttcttgcgtt ctttattttc tttgggttgc ctggtatcgt tggatttacg attcctgact
     1261 tccttgctgg tgttatcacc ttgactctta acgcaagtgc ttatatcgca gagattgttc
     1321 gaggcgggat caacgccgtt cctgtcggac aaatggaagc ttccagaagt ttaggtcttt
     1381 cttataatcg gacaatgcaa aaaatcattt tgccgcaagc gatacgcatc atgatcccat
     1441 cattcgtcaa tcagttcgtt atctcattaa aagacacaac gatcatttcc gcaatcggtg
     1501 tagtcgaatt gctgcaaact ggtaagatca ttgttgcacg tacaacacaa agtacgtatg
     1561 tttacttgat cattgcgatc atgtacttga ttttgatcac agcattgaca aaattagcaa
     1621 aagttttaga aaagaaggtg aagtaagatg gcagaaaaaa tcttagttga gcatttagtc
     1681 aaaaaatacg gcgataacac agtattaaac gacatcaatg tatccatcaa cgaaggagat
     1741 gtcgtctgta tcattggccc ttcaggttca ggaaaaagta cattcttgcg ttgcttgaat
     1801 cgtttagaag aaccttctga cggtgaaatt attattgacg gcgcccactt gatggataaa
     1861 agcacagata tcaacttggt gagacaacat atcggaatgg tattccaaca ctttaaccta
     1921 ttccctcatc tttctatttt ggaaaacatc attttagcac cgatggacct gaagaaagaa
     1981 tctagagaag aagcagagaa aaaagcaatc aaattactgc agacagttgg tctagaagat
     2041 aaaaaagata tgtatcctga aatgctttct ggtggacaaa aacagcgtgt ggctattgct
     2101 cgtgctctag cgatgaaccc tgacatcatg ctttttgatg agcctacttc tgcactcgat
     2161 cctgaaatgg tcggtgatgt cttgaacgtc atgaaaaagt tagcagaaca aggtatgacg
     2221 atggttatcg ttacgcacga aatggggttc gctaaagaag tggccaatcg cgtcatgttc
     2281 attgacgacg gaaacttttt agaagatgga acgcctcaac aaatctttga aaatccgcaa
     2341 aatgaacgga caaaagattt cttgaataaa gtattaaata tttaaaataa attatatatg
     2401 tctttttgac tatcttttta tatcttctta aatgtccgca aagccttatt ttataggctc
     2461 tacgggcatt ttgcttttgt ggtaaacctc acatatctag gtctatcttc ttatattttc
     2521 actatcaagc gtggttaaaa tcgtggtaaa tacttctatg caattagacg ctgaacctct
     2581 gattttgcgg tatctatgga cgcatgagca taccagttca ttgtgatact gatatttgaa
     2641 tgccccatga tatactgtaa atcttttggg ttcatgttct tgcttgccag tcttgtgcag
     2701 aatgtatggc gtagcgtatg cggtgtgata tgtggcaatg gattgtcctt gtggtgcttg
     2761 ttgtatttct ttaccatacg gataaataag gcgttataat caatcgcaac tttgggcttg
     2821 cccttatgat tgacaaatag gaagttgccc cgtccgtcta tcacaaatgg ttctgccttt
     2881 ggacgttttt tcataacctg ttgaaatgcc tgtatcgttt ctttgcttaa tggtacttgc
     2941 cttattccgc tttttgtttt aggcgtttca atataatagc cctgttcctt gctctttagt
     3001 aactggtggt cgataatcac aacttcattc ttgaaatcaa tgtcggctac tgtcagtccg
     3061 cacagttccg aaatacgaag tcctgtcttt aacagtatca gcacatcatc ataatgcttg
     3121 tgatacacat tgtccgtctt gatgaatgag agtaaggctt gttcctgttc ctctgtcaat
     3181 gcgactttct ttttggtatc attttctagg acttcactca acttgaaatc aaagggattt
     3241 ttcctcacat aatcatcttg aatggctatg tagaatgacg cttttaacga gcgtttatgg
     3301 ttgttaatgg tattgtagga aaagcctttg tctttcatgc gtaacgccca ttccttagcg
     3361 tcagagggtt tgattgtatc aatgctccta gcacctaact tgtcctcttt caataaccgc
     3421 atgagttgtt cccgttgttt ctgtgtgctt ttcttcacat ttgccctctg tgcgttctgt
     3481 ttggcataaa gttggcaaag cgtcattttc ttgccagtgc tgtcgatacc gtcctcaata
     3541 tcccgtctta tctgctgttc cagttctcga agtgagggtt tttcccgttt tccctttggt
     3601 gtcgggtctg tgggtgtcaa tctccaagca tacacatatt ttgtgtttcc aaatgcgtct
     3661 acatatttat ataagtattt tctgtctgtt cgttggctct ctccagtatg caggatacga
     3721 cctttgttat cccgtctttt ttctttcatg gtgtctgctc ctttccttgt tggaaagagc
     3781 cttgatatga cttgtgttca tcataacaca tacaaggctc atttgcatta gatagcgtcc
     3841 aatttatcaa tgacctgttc aaactgtcgg cgtttaatct gtatgcggtt gccattcata
     3901 atgagccagc cagcgtcctt gttttcctct gccaatcgtc ttaacttgtt ttctccgata
     3961 cggaaatact ttgacgcttc ttcaatggta agggtgtatt tttcccatac gggaatatcc
     4021 tgtgcattac tcataaaaca ccccctcact ttcgattacg tctgctatgt ccgtacacat
     4081 ctgttccaca agcatttcct gcttttcaga gaggtgggta cattcatcaa tcattttcat
     4141 gtagtttgag cctgtcagcc tgtcgatttc ctctatcatc acgattgacg gcatgacctg
     4201 tctttccagc cattgcagag tgcgttccat attgacaggt acggggttca tttcaaaagg
     4261 taaactgtca tgggaaatga atatctccca cagcggatag tcgggaaatt gcacaaaatc
     4321 agtaataagg taaaacacca gcccatgagg gttatatgtc aataacaatt cctcaactgc
     4381 ctgtactgct tttgtaccac gcagacgaat ttcaaaacga ttgataatat ccgtatgttt
     4441 ctttttggtt gcctgttcct tttgtttttc atacaggcag aagtattttg tgcttgcctt
     4501 tgaaccgata taaagggtgc ttgctaaatt acggtttttt ccgcttagtt ttccaccctg
     4561 cacattttca taatttttac agcgacaatc cgcaccgcca tttctgtatt tttcagacaa
     4621 aacgggaata tccaacagac cacacatatc attgattgcc aagtcaaaac gtcggataac
     4681 accgtcacag tcaagacagc ggtttaaaaa tgaataccaa tctctgttct gtgcctgcaa
     4741 gacatattcc atgttgcgtg agcctgcccc ttttaattcc aagaatacgc ccatgtgttc
     4801 gtcatcagaa gccatgacct tgatttctcc gtaggcgtaa tgctctttat agccataata
     4861 cccatagtca taatggataa aataatctgc tttcatgcca agcacctttc ggataatttc
     4921 caatgcgtca gtcgttggat aacggacaga cagataatca acgagcagat aaaaaggctt
     4981 atcgcagata taataattca gacaacgcag gataatattt tgtgtttcct tgctggcgtt
     5041 ggcttttccg ttctcaaagc ggttgatact atatcttgat acatgggtaa gacttgcaag
     5101 ttctccttgt gaaagaccgc ttgcaagacg tttgttcttc atttcaagtg caaattctaa
     5161 attgttcatt gtattcctcc aatcccacaa tagccttgaa atacctgtaa acctctattc
     5221 aaaaagtgtg atatgcacta tcttcttaaa acttctcatt actactcaca actttgggat
     5281 tgtatggttt ttgacttgtc ttttgtgaaa tagtgccccc ctgttagata acgggggcac
     5341 agacaaggct cgcaggctcg ccagccgaca aagcatttct tccccttgac acaaggtgtc
     5401 atggcggtaa gcctgagagg cttccgccac cgtgtcagaa atactttgtc agcaagttct
     5461 gttttgcctt ttctaacaag ttccatttca accgatattc atatctacgg atttaatcag
     5521 ataggtattt ctttttgaat gtttttatat ttcattctat tgtgcttatt cgattacaag
     5581 aacatagtaa ttcttatatg cttatttgtc aatatctttt tttgccttta caaatcatat
     5641 taagcatgat agacttataa tagcgaagat ataacgagat atggagggtt atgcaatgaa
     5701 aataagccat ttaggaaata atatacagac cataagaaaa tttaggggaa tgaaacaaca
     5761 ggaacttgcg gacaaaatcg gtatcaatat gcagagcctt tccaagattg aaagagggtt
     5821 gaactatcct gcctatgaaa cgctggaaaa gataatggaa gtgctggacg ttacgcccaa
     5881 tgaattgttg tcgggagaat ggaagtatgt caatcaatct gaaaaggaag tctgtcagtt
     5941 tttaagagcc gaagaacgtc taaatgcaga actgaaacat ggacactatg ataacttctt
     6001 tgacagcgag gaagaatggc tggaatatga gttagaaaag ttacgggaat atattacaga
     6061 ctatatcaac gggaaaagca ttgaagcgtc cgacctttac ccaatcaaag aatttatcca
     6121 gcatttgaag tttcaaaaat tattagaccg ctatgatgat ttatacagta tggatatgtt
     6181 tggggaaagc atagagggac ataagtataa gacaccttat caagtcgtga aaatgataaa
     6241 cccgaactca aaagaagata tggaactgtt acgagaagta ttaaggaata acaattttga
     6301 tgatgaggac gagtaacttt tttcctcttg cgagaagata cagcaaacaa aaagcccgat
     6361 caagagtgca aagcaccacc aatggtggca aagctcttga cagggctttt tcttttctgt
     6421 tgtgggcaat caagaggaag aaagggataa aatctgattt gtggtaaatc aggcaaaggc
     6481 aacgcaggta tctcaatttg atttggtact tgaaaagaac gggagtaatt ggaagattat
     6541 agaataacaa atattggtac attattacag ctattttgta atcacgtact ctctttgata
     6601 aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag tatctattta
     6661 aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagtggg
     6721 aacaaataat tggatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat
     6781 acctttatca tgtgattcta aagtatccgg agaatatctg tatgctttgt atgcctatgg
     6841 ttatgcataa aaatcccagt gataaaagta tttatcactg ggatttttat gcccttttgg
     6901 gtttttgaat ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg
     6961 atgcaggaaa aactacctta acagaaagct tattatataa cagtggagcg attacagaat
     7021 taggaagcgt ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag
     7081 gaattacaat tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca
     7141 tagacacgcc aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag
     7201 atggggcaat tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat
     7261 ttcatgcact taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa
     7321 atggaattga tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg
     7381 taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg
     7441 aacaatggga tacggtaata gagggaaacg atgacctttt agagaaatat atgtccggta
     7501 aatcattaga agcattggaa ctcgaacaag aggaaagcat aagatttcag aattgttctc
     7561 tgttccctct ttatcatgga agtgcaaaaa gtaatatagg gattgataac cttatagaag
     7621 ttattactaa taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg
     7681 ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc atatatacgc ctttatagtg
     7741 gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag
     7801 aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa
     7861 ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat
     7921 tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga
     7981 gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc
     8041 cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga
     8101 aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac
     8161 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
     8221 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
     8281 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcgt
     8341 ttcaaaatgc agttatggag gggatacgct atggctgtga acaaggattg tatggttgga
     8401 atgtgacgga ctgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc
     8461 cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa
     8521 cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac
     8581 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
     8641 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
     8701 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
     8761 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
     8821 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
     8881 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggagatagtt
     8941 attataaact acaagtggat attgtgtcct gtatgtggaa ataaaacacg attaaagata
     9001 agggaagata ctgaattaaa aaaattcccc ctctattgtc cgaaatgcag acaagaaaat
     9061 ttaattgaaa taaagcagtt caaagtaact gtgattacag agccagacgc aaagacgcag
     9121 agccgataaa atgagattaa tacaatctca ttttatcggc tctttccgtt atgtatggat
     9181 tcttttaatt agtcttcgat gtttcttgct tcgttgatac cgctggctaa agattccatt
     9241 aaggatagtt ctttgtctgt aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg
     9301 ctttttacca agttattagc aggtaagaaa aattcatcaa cggaaacatg aagtaacgat
     9361 acaaggtcat aaagaacttg tatgctgggg tgttgccctt tattttcaat attagttaag
     9421 taccgtgggt caatttcaat caatgctccc acttgttcac gagttaaacc tcgtttcaat
     9481 cgagcttctt taatggctaa accaaaggct ctaaaatcat atttatcttc ttttttacgc
     9541 atagtagacc acctctatac attttattgt tcctactgaa ttaaaaacag gtatagaaaa
     9601 acgtgttata tggtttatag gtttatattt aataaaaagc actactaaac gccaataaaa
     9661 aaaaccgtta tatggtagtg ctatttacgc tgttaaaata ttgtatatta cttccaaatg
     9721 gcggtttgtt ggaggtcaac gtcgccatga agtacatcat atacaataaa tttccttaca
     9781 ttgggttctt gtcaaaaaaa gtcgtctatc tgcaatagat aagtacgtcc accaatgtgg
     9841 ttttataaat catatagata gaataaca
//
LOCUS       DAZKOM010000299         9229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000299 DAZKOM010000000
VERSION     DAZKOM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.108"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8NF_003078"
     CDS             complement(134..577)
                     /locus_tag="ACG8NF_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0473449.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8NF_003079"
     CDS             complement(795..2135)
                     /locus_tag="ACG8NF_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0473450.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8NF_003080"
     CDS             2404..3300
                     /locus_tag="ACG8NF_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0473451.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8NF_003081"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8NF_003081"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0473452.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG8NF_003082"
     CDS             4517..5638
                     /locus_tag="ACG8NF_003082"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0473453.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8NF_003083"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8NF_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0473454.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG8NF_003084"
     CDS             7465..7992
                     /locus_tag="ACG8NF_003084"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0473455.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..>9229
                     /locus_tag="ACG8NF_003085"
     CDS             8203..>9229
                     /locus_tag="ACG8NF_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0473456.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAG"
BASE COUNT         3080 a         1700 c         1843 g         2606 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggta
//
LOCUS       DAZKOM010000300         8062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.117, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000300 DAZKOM010000000
VERSION     DAZKOM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.117"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8NF_003086"
     CDS             128..1564
                     /locus_tag="ACG8NF_003086"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0473457.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8NF_003087"
     CDS             1753..4200
                     /locus_tag="ACG8NF_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0473458.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8NF_003088"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8NF_003088"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0473459.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8NF_003089"
     CDS             5487..5693
                     /locus_tag="ACG8NF_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473460.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8NF_003090"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8NF_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0473461.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8NF_003091"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8NF_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0473462.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAZKOM010000301         5725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.139, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000301 DAZKOM010000000
VERSION     DAZKOM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.139"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..708
                     /locus_tag="ACG8NF_003092"
     CDS             244..708
                     /locus_tag="ACG8NF_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0473463.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            692..1321
                     /locus_tag="ACG8NF_003093"
     CDS             692..1321
                     /locus_tag="ACG8NF_003093"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0473464.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1675..3153
                     /gene="msr(C)"
                     /locus_tag="ACG8NF_003094"
     CDS             1675..3153
                     /gene="msr(C)"
                     /locus_tag="ACG8NF_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0473465.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3200..3646
                     /locus_tag="ACG8NF_003095"
     CDS             3200..3646
                     /locus_tag="ACG8NF_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0473466.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3845..4360)
                     /locus_tag="ACG8NF_003096"
     CDS             complement(3845..4360)
                     /locus_tag="ACG8NF_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0473467.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4477..5679
                     /locus_tag="ACG8NF_003097"
     CDS             4477..5679
                     /locus_tag="ACG8NF_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0473468.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1776 a          954 c         1213 g         1782 t
ORIGIN      
        1 gtaaaattat ctactgcaga agagctatct tctatagttt gataagaatc aataatcttg
       61 gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa atttcttttc
      121 tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt gacttttttc
      181 tttttttatt ctattattga caagtcaatc gttgacgagt caattattag aaaggaggaa
      241 attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa
      301 atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga gatcatgatg
      361 agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt gcagattgat
      421 caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag
      481 agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa
      541 atcaagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc ggctttcagc
      601 gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca actttgcaat
      661 ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat
      721 tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga
      781 aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgaaaccatg
      841 gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt
      901 caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg
      961 ttacgaatat ggcgaagaaa tctacaacca agcttacgaa tcagggaaga tgccaaaaga
     1021 agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga
     1081 aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc
     1141 gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc agttggcaga
     1201 ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc
     1261 ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta tgttcgaata
     1321 aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta
     1381 ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat
     1441 aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc
     1501 attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata
     1561 gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg
     1621 ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa
     1681 aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct
     1741 attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc
     1801 ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc
     1861 caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac
     1921 ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa
     1981 tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa
     2041 gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt
     2101 gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta
     2161 gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg
     2221 aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca
     2281 gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa
     2341 gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag actggatcgg
     2401 ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca
     2461 ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc
     2521 cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat
     2581 gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta
     2641 ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat
     2701 atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag
     2761 caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca
     2821 gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact
     2881 ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg
     2941 ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg
     3001 gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact
     3061 tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag
     3121 aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa
     3181 tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat
     3241 gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct
     3301 tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga
     3361 tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga
     3421 aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg
     3481 tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta
     3541 tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg
     3601 cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat
     3661 ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat
     3721 agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta
     3781 ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag gttctgttta
     3841 tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct
     3901 gctgacaaag cataattctg cgctggatca caacataacg tccaaattct tgtttcttgt
     3961 ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc agctgttgtt
     4021 ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt
     4081 ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc cattcctcta
     4141 caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt
     4201 ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag atctgactgt
     4261 gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa cgtggtttgg
     4321 ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta cctctcgtct
     4381 tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata ttgacatgta
     4441 aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga gaatcaaata
     4501 gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct agttgtagca
     4561 gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt aaaaacgatt
     4621 gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag ttacatgtta
     4681 gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa
     4741 acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg
     4801 aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc
     4861 tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg
     4921 atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg
     4981 ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt
     5041 atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg
     5101 gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta
     5161 tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct
     5221 gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat tgcttcgatt
     5281 ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt agcagcattt
     5341 gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc agtccttgta
     5401 ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact gttgacgacg
     5461 attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta tagttttgtc
     5521 cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga acatattggt
     5581 gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt
     5641 tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa
     5701 gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAZKOM010000302         4800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.151, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000302 DAZKOM010000000
VERSION     DAZKOM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.151"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8NF_003098"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8NF_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0473469.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8NF_003099"
     CDS             763..1917
                     /locus_tag="ACG8NF_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0473470.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8NF_003100"
     CDS             2132..3100
                     /locus_tag="ACG8NF_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0473471.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8NF_003101"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8NF_003101"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0473472.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8NF_003102"
     CDS             4130..4738
                     /locus_tag="ACG8NF_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0473473.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          920 c         1119 g         1280 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
//
LOCUS       DAZKOM010000303         3961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000303 DAZKOM010000000
VERSION     DAZKOM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.165"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACG8NF_003103"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACG8NF_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACG8NF_003104"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACG8NF_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACG8NF_003105"
     CDS             complement(1055..1345)
                     /locus_tag="ACG8NF_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473474.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1747..1983
                     /locus_tag="ACG8NF_003106"
     CDS             1747..1983
                     /locus_tag="ACG8NF_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0473475.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1990..3942
                     /locus_tag="ACG8NF_003107"
     CDS             1990..3942
                     /locus_tag="ACG8NF_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0473476.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1386 a          668 c          690 g         1217 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt
     1681 gtaggaatgc aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat
     1741 tttcctatgc aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata
     1801 tcagagcaag taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc
     1861 gaagcagtgg atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt
     1921 gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt
     1981 tgatagacaa tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt
     2041 aaaattccga tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact
     2101 ccaaaacgat ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg
     2161 aacggttctg aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt
     2221 gggaaagaac agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact
     2281 ggtagttcca aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga
     2341 ggtgtatcgc aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt
     2401 aaacgcctta tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa
     2461 gaaggctgct cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg
     2521 gggaaaagta ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat
     2581 gttctaccaa aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca
     2641 atgttcgcta agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga
     2701 attggagttg gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt
     2761 ttagaacgat atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat
     2821 acggacaaga tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa
     2881 tatacggggt ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta
     2941 tcaagatatg tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta
     3001 aatgaagata caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat
     3061 aggttagatt ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat
     3121 gcttatcttc acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat
     3181 gttgatgctc cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc
     3241 gacgaatggc gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta
     3301 ttcacgaaag aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg
     3361 aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg
     3421 ggagatatca gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact
     3481 gaattactaa caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat
     3541 cgcaaatatg ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat
     3601 tcagttgatt gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta
     3661 ttaaatgtta atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca
     3721 tctcttgaaa gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac
     3781 ttcaatccga agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt
     3841 cgccctttta aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca
     3901 gataaacaat ttgattggaa agatataatt tatttcaaat gacaaaaaga gcttggagta
     3961 a
//
LOCUS       DAZKOM010000304         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.176, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000304 DAZKOM010000000
VERSION     DAZKOM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.176"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG8NF_003108"
     CDS             3..188
                     /locus_tag="ACG8NF_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0473477.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG8NF_003109"
     CDS             203..1072
                     /locus_tag="ACG8NF_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0473478.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG8NF_003110"
     CDS             1053..1787
                     /locus_tag="ACG8NF_003110"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0473479.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG8NF_003111"
     CDS             1820..2728
                     /locus_tag="ACG8NF_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0473480.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG8NF_003112"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG8NF_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKOM010000305         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.236, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000305 DAZKOM010000000
VERSION     DAZKOM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.236"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8NF_003113"
     CDS             26..76
                     /locus_tag="ACG8NF_003113"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0473481.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8NF_003114"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8NF_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0473482.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DAZKOM010000306          658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3438
            SAMEA115706716-rid22575693.guided.272, whole genome shotgun
            sequence.
ACCESSION   DAZKOM010000306 DAZKOM010000000
VERSION     DAZKOM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706716
            Sequence Read Archive: ERR13196451
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287744.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287744.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:00:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706716-rid22575693.guided.272"
                     /isolate="VRE3438"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..607
                     /gene="dfrG"
                     /locus_tag="ACG8NF_003115"
     CDS             110..607
                     /gene="dfrG"
                     /locus_tag="ACG8NF_003115"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0473483.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          248 a           69 c          140 g          201 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcat aatgagaaaa ggttttttga
       61 tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc
      121 tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag
      181 gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg
      241 taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac
      301 gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag aagatgtttt
      361 tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga tttataattt
      421 gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat tcgaaggaga
      481 tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc aaaaagggat
      541 aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa aaaacttatt
      601 gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag agcttggg
//
